CN102197819B - Bactericidal composition and application thereof - Google Patents
Bactericidal composition and application thereof Download PDFInfo
- Publication number
- CN102197819B CN102197819B CN 201110084964 CN201110084964A CN102197819B CN 102197819 B CN102197819 B CN 102197819B CN 201110084964 CN201110084964 CN 201110084964 CN 201110084964 A CN201110084964 A CN 201110084964A CN 102197819 B CN102197819 B CN 102197819B
- Authority
- CN
- China
- Prior art keywords
- bactericidal composition
- blight
- composition
- ingredient
- metalaxyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses a bactericidal composition and its application, the effective component comprises an ingredient A and an ingredient B. The ingredient A is metalaxyl-M, the ingredient B is selected from one of polyoxin, Ningnanmycin and validamycin. The invention has the advantages of high efficiency, safety and environmental protection. The bactericidal composition has synergistic effect which is characterized in that the product output can be raised, the cost can be reduced and the generation of drug resistance of germs can be delayed. The invention can be used for preventing and treating sheath blight, root rot, rice blast, corn northern and southern leaf blight, downy mildew, phytophthora blight, leaf spot, botrytis, alternaria leaf spot, anthracnose, ring spot, powdery mildew, black shank and late blight.
Description
Technical field
The present invention relates to a kind of bactericidal composition and uses thereof.
Background technology
At present, in the pest chemical control, pest pesticide resistance problem is very serious.The fungus-caused disease of Oomycete occurs generally often to cause heavy losses in agricultural production.Metalaxyl-M is widely used in the control of corps diseases as a kind of bactericide with significant protection, treatment and eradicant action, has good control efficiency.But existing research thinks that there is high resistance risk in Metalaxyl-M to greenhouse, greenhouse crops disease.There is the complex composition of synergistic effect to reduce to a certain extent resistance risk, avoids producing the speed of resistance and delaying drug resistance generation.
It is the metabolite that streptomyces aureus produces that polyoxin is produced in China, belongs to the broad-spectrum antibiotics series bactericidal agent.Absorption in it has is preferably disturbed the biosynthesis of somatic cells wall, can also suppress germ and produce spore and scab expansion.Different from the bactericidal mechanism of Metalaxyl-M, without cross resistance.Production practices through for many years prove, no matter are what crop to use many anti-mildews toxin, not only do not find people and animals' intoxication accident, also do not find the contaminated environment phenomenon, and the equal non agricultural chemical residuum of agricultural byproducts.
Summary of the invention
The object of the present invention is to provide bactericidal composition of a kind of efficient, safety, environmental protection and uses thereof.
Technical solution of the present invention is:
A kind of bactericidal composition is characterized in that: active ingredient is comprised of two kinds of A and B, and composition A is Metalaxyl-M, and composition B is selected from a kind of compound in polyoxin, Ningnanmycin, the jinggangmeisu.
The mass ratio of effective constituents A and B is 60: 1～1: 60.
The gross mass percentage composition of active ingredient in bactericidal composition is 1～80%.
The formulation of described microbicide compositions is water dispersible granules, aqua, wetting powder, suspending agent, missible oil, aqueous emulsion or granule.
The application of a kind of microbicide compositions in the medicine of preparation control crop banded sclerotial blight, root rot, rice blast, corn northern and southern leaf blight, downy mildew, epidemic disease, leaf spot, gray mold, spot defoliation, anthracnose, ring spot, powdery mildew, balck shank, late blight.
Described crop is paddy rice, wheat, corn, vegetables, fruit tree, melon and fruit, tobacco or potato.
The present invention is efficient, safety, environmental protection, and composition has synergistic effect, can improve crop yield, reduce cost and be of value to and delay the drug-fast generation of germ.
The invention will be further described below in conjunction with embodiment.
Embodiment
Embodiment 1:
With Metalaxyl-M 4g, polyoxin 8g, calcium dodecyl benzene sulfonate 6g, phenethyl phenol polyethenoxy ether 4g, dimethylbenzene adds to 100g, is mixed to homogeneous phase liquid, makes composition missible oil.
Embodiment 2:
With Metalaxyl-M 5g, polyoxin 10, sodium lignin sulfonate 8g, diffusant sodium methylene bis-naphthalene sulfonate 4g, White Carbon black adds to 100g, and mixture carries out air-flow crushing, makes wetting powder.
Embodiment 3:
With Metalaxyl-M 2g, polyoxin 4g, phenethyl phenol formaldehyde resin APEO 3g, basic phenol mela mine-formaldehyde resin APEO 2g, antifreeze ethylene glycol, glycerine 5g, dispersant fatty alcohol polyoxy ethyl ether 5g, antisettling agent xanthans 1g, suitable quantity of water, mix, through wet grinding, homogenizing makes aqueous suspension agent.
Embodiment 4
With Metalaxyl-M 2g, polyoxin 16g, phenethyl phenol formaldehyde resin APEO 3g, basic phenol mela mine-formaldehyde resin APEO 2g, antifreeze ethylene glycol, glycerine 5g, dispersant fatty alcohol polyoxy ethyl ether 5g, antisettling agent xanthans 1g, suitable quantity of water is mixed, through the homogenizer high speed shear, make aqueous emulsion.
Embodiment 5
With Metalaxyl-M 5g, polyoxin 10g, dispersant fatty alcohol polyoxy ethyl ether 5g, polyoxyethylene nonylphenol ether 3g, starch 1.2g, sodium bicarbonate 1g, bentonite 12g, kaolin adds to 100, mixes, and makes the dispersibility granula through pulverizing granulation.
Biological example:
The synergistic function of the present composition can illustrate by following example, but the present invention only limits to by no means this.
Take by weighing an amount of reactive compound acetone solution, water is mixed with each compound the solution of described concentration, mixes by described proportioning equal proportion, obtains described proportioning and concentration.
The EC of each medicament is obtained in linear regression analysis between probit value by inhibiting rate and the logarithm value of serial drug concentration
50, the interaction of calculating mixed agent according to the method for Wadley, A, B represent respectively two medicament components, and a|, b are the ratios of each component content in mixture.Analyze the effect be mixed with the SR value.Wherein: SR=0.5～1.5, two kinds of medicaments addition that has been mixed then, SR≤0.5, two kinds of medicaments antagonism that has been mixed then, SR 〉=1.5, then two kinds of medicaments synergistic effect that has been mixed.
The computational methods of SR value are:
EC
50(theoretical value)=(a+b)/(a/EC
50a+ b/EC
50b)
SR=EC
50(theoretical value)/EC
50(real value)
1, to the effect of apple tree spot defoliation.
The mycelial growth rate method is adopted in test.The previously prepared inoculated by hypha block of picking is on the EPA of different drug concentrations culture medium flat plate, 25 ℃ of lower cultivations 7 days, check colony diameter, calculate the percentage that each chemicals treatment suppresses mycelial growth, the EC of each medicament is obtained in linear regression analysis between probit value by inhibiting rate and the logarithm value of serial drug concentration
50, the interaction of calculating mixed agent according to the method for Wadley.
The reactive compound that table 1 is independent
| Embodiment | Reactive compound | EC 50μ g/ml (theoretical value) | EC 50μ g/ml (observed value) | SR 50 |
| 1 | The I Metalaxyl-M | 1.3056 | ||
| 2 | The II polyoxin | 0.7043 | ||
| 3 | The III Ningnanmycin | 1.1209 | ||
| 4 | The IV jinggangmeisu | 1.4532 |
Table 2 mixture of the present invention
SR 〉=1.5, showing has obvious synergistic effect after both are mixed.
2, to the effect of black shank.
The mycelial growth rate method is adopted in test.The previously prepared inoculated by hypha block of picking is on the EPA of different drug concentrations culture medium flat plate, 22 ℃ of lower cultivations 6 days, check colony diameter, calculate the percentage that each chemicals treatment suppresses mycelial growth, the EC of each medicament is obtained in linear regression analysis between probit value by inhibiting rate and the logarithm value of serial drug concentration
50, the interaction of calculating mixed agent according to the method for Wadley.
The reactive compound that table 3 is independent
| Embodiment | Reactive compound | EC 50μ g/ml (theoretical value) | EC 50μ g/ml (observed value) | SR 50 |
| 16 | The I Metalaxyl-M | 0.02 | ||
| 17 | The II polyoxin | 3.7681 | ||
| 18 | The III Ningnanmycin | 2.4765 | ||
| 19 | The IV jinggangmeisu | 4.981 |
Table 4 mixture of the present invention
SR 〉=1.5, showing has obvious synergistic effect after both are mixed.
3, to the effect of potato late blight.
Phytophthora infestans separation from Shandong Province's potato leaf, purifying, evaluation obtain.Before measuring on the rye culture medium flat plate 18 ℃ of lower precultures 10 days, beat cut-off footpath 5mm mycelia piece at colony edge and be used for mensuration.The previously prepared inoculated by hypha block of picking is on the culture medium flat plate of different drug concentrations, mycelia faces up, check colony diameter 18 ℃ of lower cultivations after 12 days, calculate the percentage that each chemicals treatment suppresses mycelial growth, the EC of each medicament is obtained in linear regression analysis between probit value by inhibiting rate and the logarithm value of serial drug concentration
50, the interaction of calculating mixed agent according to the method for Wadley.
The reactive compound that table 5 is independent
| Embodiment | Reactive compound | EC 50μ g/ml (theoretical value) | EC 50μ g/ml (observed value) | SR 50 |
| 31 | The I Metalaxyl-M | 0.0057 | ||
| 32 | The II polyoxin | 0.7890 | ||
| 33 | The III Ningnanmycin | 0.8673 | ||
| 34 | The IV jinggangmeisu | 5.3712 |
Table 6 mixture of the present invention
SR 〉=1.5, showing has obvious synergistic effect after both are mixed.
4, to the effect of capsicum epidemic disease
The previously prepared capsicum epidemic disease mycelia of picking piece is inoculated on the EPA culture medium flat plate of different drug concentrations, mycelia faces up, 22 ℃ of lower cultivations 5 days, check colony diameter, calculate the percentage that each chemicals treatment suppresses mycelial growth, the EC of each medicament is obtained in linear regression analysis between probit value by inhibiting rate and the logarithm value of serial drug concentration
50, the interaction of calculating mixed agent according to the method for Wadley.
The reactive compound that table 7 is independent
| Embodiment | Reactive compound | EC 50μ g/ml (theoretical value) | EC 50μ g/ml (observed value) | SR 50 |
| 46 | The I Metalaxyl-M | 0.032 | ||
| 47 | The II polyoxin | 2.1692 | ||
| 48 | The III Ningnanmycin | 1.5690 | ||
| 49 | The IV jinggangmeisu | 2.3580 |
Table 8 mixture of the present invention
SR 〉=1.5, showing has obvious synergistic effect after both are mixed.
5, to the cucumber downy mildew effect
The mycelial growth rate method is adopted in test.The previously prepared inoculated by hypha block of picking is on the culture medium flat plate of different drug concentrations, 25 ℃ of lower cultivations 3 days, check colony diameter, calculate the percentage that each chemicals treatment suppresses mycelial growth, the EC of each medicament is obtained in linear regression analysis between probit value by inhibiting rate and the logarithm value of serial drug concentration
50, the interaction of calculating mixed agent according to the method for Wadley.
The reactive compound that table 9 is independent
| Embodiment | Reactive compound | EC 50μ g/ml (theoretical value) | EC 50μ g/ml (observed value) | SR 50 |
| 61 | The I Metalaxyl-M | 3.6800 | ||
| 62 | The II polyoxin | 4.5690 | ||
| 63 | The III Ningnanmycin | 4.4894 | ||
| 64 | The IV jinggangmeisu | 6.7901 |
Table 10 mixture of the present invention
SR 〉=1.5, showing has obvious synergistic effect after both are mixed.
6, to the effect of phytophthora root rot of soybean
Phytophthora sojae kaufmann﹠gerdemann is provided by Nanjing agricultural university fungi laboratory, cultivates 8 days under 22 ℃ on the lima bean culture medium flat plate before measuring, and beats cut-off footpath 5mm mycelia piece at colony edge and is used for measuring.The mycelia piece that picking has prepared is dressed seed on the culture medium flat plate that contains different chemicals treatment, and mycelia faces up, and checks colony diameter 22 ℃ of lower cultivations after 8 days.The EC of each medicament is obtained in linear regression analysis between probit value by inhibiting rate and the logarithm value of serial drug concentration
50, the interaction of calculating mixed agent according to the method for Wadley.
The reactive compound that table 11 is independent
| Embodiment | Reactive compound | EC 50μ g/ml (theoretical value) | EC 50μ g/ml (observed value) | SR 50 |
| 76 | The I Metalaxyl-M | 0.0320 | ||
| 77 | The II polyoxin | 0.9086 | ||
| 78 | The III Ningnanmycin | 0.8730 | ||
| 79 | The IV jinggangmeisu | 1.6570 |
Table 12 mixture of the present invention
SR 〉=1.5, showing has obvious synergistic effect after both are mixed.
7, the effect of rice seedling blight
Rice wilt pathogens separation, purifying and carry out tieback and identify and obtain from the withered seedling of browning experimental field.Before measuring on the PSA culture medium flat plate 25 ℃ of lower preculture 30h, beat cut-off footpath 5mm mycelia piece at colony edge and be used for mensuration.The mycelia piece that picking has prepared is dressed seed on the culture medium flat plate that contains different chemicals treatment, and mycelia faces up, and checks colony diameter 25 ℃ of lower cultivations after 8 days.The EC of each medicament is obtained in linear regression analysis between probit value by inhibiting rate and the logarithm value of serial drug concentration
50, the interaction of calculating mixed agent according to the method for Wadley.
The reactive compound that table 3 is independent
| Embodiment | Reactive compound | EC 50μ g/ml (theoretical value) | EC 50μ g/ml (observed value) | SR 50 |
| 91 | The I Metalaxyl-M | 0.0383 | ||
| 92 | The II polyoxin | 1.9851 | ||
| 93 | The III Ningnanmycin | 2.0946 | ||
| 94 | The IV jinggangmeisu | 3.7649 |
Table 4 mixture of the present invention
SR 〉=1.5, showing has obvious synergistic effect after both are mixed.
Application method: foliar spray, seed treatment, the dispenser of pouring root.
Claims (4)
1. bactericidal composition is characterized in that: active ingredient is comprised of two kinds of A and B, and composition A is Metalaxyl-M, and composition B is polyoxin; The mass ratio of effective constituents A and B is 10:1～1:5.
2. bactericidal composition according to claim 1, it is characterized in that: the gross mass percentage composition of active ingredient in bactericidal composition is 1～80%.
3. bactericidal composition according to claim 1, it is characterized in that: the formulation of described microbicide compositions is water dispersible granules, aqua, wetting powder, suspending agent, missible oil, aqueous emulsion or granule.
4. the application of bactericidal composition claimed in claim 1 in the medicine of preparation control apple tree spot defoliation, black shank, potato late blight, capsicum epidemic disease, cucumber downy mildew, phytophthora root rot of soybean, rice seedling blight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110084964 CN102197819B (en) | 2011-04-06 | 2011-04-06 | Bactericidal composition and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110084964 CN102197819B (en) | 2011-04-06 | 2011-04-06 | Bactericidal composition and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102197819A CN102197819A (en) | 2011-09-28 |
| CN102197819B true CN102197819B (en) | 2013-01-02 |
Family
ID=44659009
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110084964 Active CN102197819B (en) | 2011-04-06 | 2011-04-06 | Bactericidal composition and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102197819B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103348978A (en) * | 2012-04-10 | 2013-10-16 | 江苏启和化工有限公司 | Metalaxyl-M and acetochlor composite missible oil |
| CN103371189B (en) * | 2012-04-17 | 2017-03-15 | 长江大学 | A kind of bactericidal composition containing antibiotic in pyrimidine nucleoside class |
| WO2015160619A1 (en) * | 2014-04-16 | 2015-10-22 | Bayer Cropscience Lp | Compositions comprising ningnanmycin and a fungicide |
| CN107462652B (en) * | 2016-06-06 | 2021-01-01 | 谱尼测试集团上海有限公司 | Method for determining residual quantity of ningnanmycin in vegetable food |
| CN109303054A (en) * | 2018-11-22 | 2019-02-05 | 青岛中达农业科技有限公司 | A kind of fungicide containing kasugarnycin and Metalaxyl-M |
| CN109769843A (en) * | 2019-03-11 | 2019-05-21 | 西华大学 | A kind of bactericidal composition containing metalaxyl, pesticide and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101536685A (en) * | 2008-03-18 | 2009-09-23 | 北京绿色农华植保科技有限责任公司 | Method for preparing polyoxin aqueous solution |
| CN101601392A (en) * | 2009-06-25 | 2009-12-16 | 车志忠 | Built disinfectant |
-
2011
- 2011-04-06 CN CN 201110084964 patent/CN102197819B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101536685A (en) * | 2008-03-18 | 2009-09-23 | 北京绿色农华植保科技有限责任公司 | Method for preparing polyoxin aqueous solution |
| CN101601392A (en) * | 2009-06-25 | 2009-12-16 | 车志忠 | Built disinfectant |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102197819A (en) | 2011-09-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102204550B (en) | Sterilization composite containing metalaxyl-M and application thereof | |
| CN102197819B (en) | Bactericidal composition and application thereof | |
| CN103155925A (en) | Sterilization composite containing phenazino-1-carboxylic acid | |
| CN103155936A (en) | Plant disease control composition containing phenazino-1-carboxylic acid | |
| CN103120166B (en) | Hymexazol-containing sterilizing composition | |
| CN102669100B (en) | Oxine-copper and validamycin compound pesticide composition and preparation method and application thereof | |
| CN104430370A (en) | Fungicidal composition | |
| CN102696624A (en) | Bactericidal composition containing difenoconazole and kresoxim-methyl | |
| CN102715179A (en) | Sterilization composition containing cyazofamid and pyraclostrobine and application thereof | |
| CN102669114B (en) | A kind of prothioconazoles that contains is with the composition pesticide of hymexazo | |
| CN103329905B (en) | Sterilization composition containing penflufen and validamycin | |
| CN103478153B (en) | A kind of bactericidal composition containing Boscalid and benzene metsulfovax | |
| CN102318641A (en) | Bactericidal compound containing fenoxanil | |
| CN101258854B (en) | Carbamate and protective fungicide composition and use | |
| CN104247709B (en) | A kind of bactericidal composition of synergy and application thereof | |
| CN104430390A (en) | Amino-oligosaccharin and ethaboxam compounded agricultural fungicide | |
| CN104430348A (en) | Sterilization composition containing kasugamycin and validamycin | |
| CN104396972A (en) | Compound bactericide | |
| CN103651440A (en) | Complex bactericide | |
| CN102388893B (en) | Pesticide composition containing ZJ0712 and tetraconazole | |
| CN103651398B (en) | Compound bactericide | |
| CN103651409A (en) | Bactericidal composition containing hymexazol and oligochitosan and application thereof | |
| CN102696647A (en) | Sterilizing composite containing pyraclostrobin and pyrimethanil and application | |
| CN110583669B (en) | Bactericidal composition for melon diseases | |
| CN107223661A (en) | A kind of composition pesticide containing DCPTA |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |