CN103636619A

CN103636619A - Compound bactericide

Info

Publication number: CN103636619A
Application number: CN201310627295.0A
Authority: CN
Inventors: 杨会营; 刘晓娇; 王建华; 吴勇
Original assignee: Beijing Yanhua Yongle Biotechnology Co Ltd
Current assignee: Beijing Yanhua Yongle Biotechnology Co Ltd
Priority date: 2013-11-28
Filing date: 2013-11-28
Publication date: 2014-03-19

Abstract

The invention belongs to the technical field of pesticides, and relates to a compound bactericide. The compound bactericide is characterized by comprising the active components of tetramycin and fludioxonil in a ratio of 1:100 to 100:1. The composition can be prepared into water dispersible granules or a suspending agent, is used for preventing and treating take-all disease of wheat, has a remarkable synergetic effect, and has a long-lasting effect and an environment-friendly effect.

Description

A kind of composite bactericide

Technical field

The invention belongs to technical field of pesticide, contain specifically the composite bactericide with synergistic effect of tetramycin and fludioxonil.

Background technology

Tetramycin (Tetramycin) claim again tetramycin, is the not stimulation metabolite of streptomyces hygroscopicus Wuzhou subspecies (Streptomyces ahygroscopicn wuzhouensisn subsp.) of the strain that separates in the soil sample of Wuzhou, Guangxi.This product fungicidal spectrum is wide, and three main kind of 26 kinds of known disease funguses such as mastigomycetes, sac fungi and Deuteromycotina fungi are all had to extremely strong killing action.The control of the applicable multiple fungal disease of various crops.Especially to fruit tree putrefaction disease, spot defoliation; Cotton yellow (withered) disease of withering; Root rot; Rice sheath blight disease, seedling blight; Ginseng and pseudo-ginseng black spot; Tealeaves tea gall; Fruit white rot of grape, the climing rot of watermelon, root rot, the rotten special efficacy of stem foot.This composition can obviously promote callus healing simultaneously, promotes weak seedling well developed root system, the recovery of aging root system, improves crop disease-resistant ability and optimizes crop quality.

Fludioxonil (Fludioxonil) another name: fludioxonil; The bacterium that do not fall is disliked; 4-(2,2-bis-fluoro-1,3-benzo dioxy-4-yl) pyrroles-3-nitrile; 4-(2,2-bis-fluoro-1,3-benzo dioxolanes-4-yl) pyrroles-3-nitrile.This product is novel wide spectrum, non-interior suction pyroles bactericide, and the microbial kind biography of many cause of diseases such as sac fungi, basidiomycetes, imperfect fungus and soil-borne disease are had to extraordinary preventive effect.It suppresses mycelial growth by suppressing the relevant transhipment of glucose phosphorylated, finally causes germ dead.The mechanism of action that it is unique, does not have cross resistance with other known bactericide.Suitable crop wheat, barley, corn, pea, rape, paddy rice, ornamental crops, xylocarp, vegetables Lay, grape and lawn etc., be used for preventing and treating the rotten Fusariumsp of snow, wheat net bunt bacterium, rhizoctonia solani etc. as blade face bactericide, and gray mold is had to special efficacy; As seed treatment: be mainly used in cereal and fragrant-flowered garlic cereals crop that control kind passes and soil-borne disease bacterium as Alternaria, Ascochyta, aspergillus, Fusariumsp bits, length wriggle spore bits, Rhizoctonia and Penicillium bacterium etc.

The agricultural chemicals using mostly at present is synthetic, and the intermediate that has a lot of high poison in synthetic overweight process produces, and the impact that the use of a large amount of chemical pesticides has increasingly sharpened on environment.For current present situation, people start to be more conceived to the more free of contamination biogenic pesticide of low toxic and environment-friendly, because biogenic pesticide is to utilize the antagonist properties between microorganism to prevent and treat pathogen, this is more efficient and environmental protection than existing chemosynthesis composition, new varieties and the complex preparation of exploitation biogenic pesticide are more and more subject to people's attention at present, and in the present invention, relate to tetramycin effectively become biogenic pesticide, and fludioxonil is one of product of the whole world acquisition few in number EPA of EPA " low-risk " authentication, to crop and Environmental security.We are composite by tetramycin and fludioxonil, to strengthening both drug effects, expand fungicidal spectrum, reduce peasant's labour cost.

Summary of the invention

The object of this invention is to provide a kind of composite bactericide with synergistic effect and safety and environmental protection, realize the object that improves drug effect, reduces pollution, reduces costs.Be specifically related to the composite bactericide of tetramycin and fludioxonil.

Utilize above-mentioned composite bactericide, be equipped with the known auxiliary agent of those skilled in that art, with the known method of those skilled in that art, make formulation of the present invention, comprise suspending agent and water dispersible granules.

In above-mentioned composite bactericide, the weight ratio of tetramycin and fludioxonil is 1:100-100:1.

Above-mentioned composite bactericide has following characteristics: 1. tetramycin is biogenic pesticide, little to environmental influence; 2. fludioxonil mechanism of action uniqueness is difficult for making fungi to produce resistance; 3. formulation environmental protection, does not add organic principle; 4. two kinds of compositions are composite, have synergistic effect; 5. twenty percent divide between without cross resistance; 6. delay the generation of active ingredient resistance, reduce peasant's labour cost; 7. the lasting period long, reduce spraying times.

Embodiment

For checking tetramycin and two kinds of composite synergistic effects of active ingredient of fludioxonil, first applicant uses these two kinds of compositions to carry out indoor biometrics test, and test method and result are as follows:

First analyzed after the effect feature of two kinds of active ingredients, we are decided to be gaeumannomyce wheat variety (Gaeumannomyces graminis (Sacc.) Arx et Oliver var.tritici (Sacc.) Walker) by the raw object of surveying and belong to Ascomycotina Gaeumannomyces fungi, on PDA medium, bacterium colony is white just, after gray and black, aerial hyphae grey, short and intensive, this bacterium can cause the full rot of wheat.

The test method of indoor biometrics: adopt growth rate method to measure the inhibitory action of medicament to the growth of take-all mycelia.On the basis of preliminary experiment, from each medicament, germ mycelial growth inhibition rate is reached and within the scope of 10%-90%, designs 5 concentration, first the former medicine of 95% tetramycin and the former medicine of 70% fludioxonil be take methyl alcohol as the liquid that solvent is made into series concentration standby, then by liquid in 1% ratio join melt and the PDA medium of cool to room temperature in, after fully shaking up, pour that in the culture dish (Φ 90mm) of sterilizing, to make band medicine dull and stereotyped into, every processing repeats for four times, to add the blank that is treated to of sterile water; Card punch (Φ 5mm) picking gaeumannomyces graminis bacterium cake with sterilizing, mycelia faces down aseptic inoculation in the central authorities with medicine flat board, be inverted in interior cultivation of constant incubator of 25 ℃, after cultivation 6d, adopt right-angled intersection method to measure colony diameter, calculate the EC of each medicament to pathogen ₅₀, and calculate according to the following equation toxicity index, the virulence of more different medicaments and bacteriostasis.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.

The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:

The theoretical toxicity index TTI=∑ of mixture preparation (the toxicity index ATI of certain medicine * percentage of this pharmaceutically active ingredient in mixture).

It is synergistic effect that co-toxicity coefficient CTC is greater than at 120 o'clock, and being less than at 80 o'clock is antagonism, is addition in the time of between 80-120.

Indoor biometrics test data sees the following form:

Table 1 tetramycin, fludioxonil and the measurement result of different proportioning to take-all co-toxicity coefficient thereof

Analyze above-mentioned data, can obviously find out when tetramycin and fludioxonil are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 120, the prevention and control capability of take-all is had to the synergistic effect of different proportion, wherein tetramycin and fludioxonil are being that proportioning five is visible to the synergy between proportioning seven between 1:5-5:1, the composite desirable medicament of preventing and treating take-all that becomes of tetramycin and fludioxonil.

By indoor biometrics, test and determine tetramycin and two kinds of active ingredients of fludioxonil are composite can good synergistic effect, below by instantiation in order to further describe the present invention, but the present invention absolutely not only only limits to following these embodiment.In formula, percentage is all weight percentage, and the former medicine using and auxiliary agent are other producers and buy.

Formulation examples is as follows:

Example one, 10.1% tetramycin fludioxonil suspending agent

Example two, 22% tetramycin fludioxonil water dispersible granules

Example three, 24% tetramycin fludioxonil suspending agent

Example four, 36% tetramycin fludioxonil water dispersible granules

Example five, 8% tetramycin fludioxonil suspending agent

Example six, 15.3% tetramycin fludioxonil water dispersible granules

Example seven, 20.2% tetramycin fludioxonil suspending agent

Example eight, 15.5% tetramycin fludioxonil water dispersible granules

Example nine, 18.6% tetramycin fludioxonil suspending agent

Select above example to prevent and treat the field trial of take-all, the formula of determining is prevented and treated the effect of take-all; Simultaneously by the Contrast on effect with 10% tetramycin SC and 10% fludioxonil SC, verify the synergistic effect after composite.Concrete test method data are as follows:

8% tetramycin fludioxonil suspending agent and other preparation are done the test of pesticide effectiveness of field control take-all.Test site is Handan City, Hebei, and the time is on April 3rd, 2012, test each and process three repetitions, and community randomized arrangement, each community area is 60m ², adopting back-pack electric compression sprayer, dispenser was sunny calm the same day, and the planting habit of all communities is all identical with local planting habit, records the dispenser same day and the synoptic data of a week thereafter, specific as follows:

Table 2 duration of test meteorological data

Investigation method is: each community adopts five point samplings, gets one square metre at every, and after dispenser, fixed point investigation in the 7th day, 21 days, investigates each community disease and refer to, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.

The grade scale of disease index:

0 grade, anosis;

1 grade, blackening root area accounts for below 5% of the root gross area;

2 grades, blackening root area accounts for 6%～10% of the root gross area;

3 grades, blackening root area accounts for 11%～25% of the root gross area;

4 grades, blackening root area accounts for 26%～40% of the root gross area;

5 grades, blackening root area accounts for 41%～65% of the root gross area;

6 grades, blackening root area accounts for 66%～100% of the root gross area.

Table 3 8% tetramycin fludioxonil suspending agent and the preventive effect of different medicament to take-all

Note: in table, data are the mean value time repeating for 3 times: in April, 2012 place; Handan, Hebei

From above-mentioned data, can find out, tetramycin and fludioxonil complex preparation after medicine 7 days preventive effects all over 78%, and the preventive effect of 7 days is that after 47.68%, 10% fludioxonil SC medicine, the preventive effect of 7 days is 45.92% after 10% tetramycin SC medicine, visible complex preparation is compared synergistic effect with single dose extremely remarkable.And after medicine 21 days, all complex preparations have all surpassed 85% to the preventive effect of take-all.Complex preparation not only can improve drug effect, extends the lasting period, and alleviates peasant's labour cost, has very high economic benefit.

Claims

1. a composite bactericide, is characterized in that: active component is comprised of tetramycin and fludioxonil, and the weight ratio of described tetramycin and fludioxonil is 1:100～100:1.

2. composite bactericide according to claim 1, is characterized in that: by active ingredient and auxiliary material, make suspending agent, water dispersible granules.

3. according to claim 1 or composite bactericide claimed in claim 2, it is characterized in that: for preventing and treating take-all.