CN103907644A - Sterilization composite - Google Patents
Sterilization composite Download PDFInfo
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- CN103907644A CN103907644A CN201410168470.9A CN201410168470A CN103907644A CN 103907644 A CN103907644 A CN 103907644A CN 201410168470 A CN201410168470 A CN 201410168470A CN 103907644 A CN103907644 A CN 103907644A
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- antibiotic
- pyrimidine nucleoside
- nucleoside class
- piperazine mycin
- composite
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Abstract
The invention belongs to the technical field of pesticides and relates to a sterilization composite. The sterilization composite is characterized in that the active ingredients of the sterilization composite include phenazino-1-carboxylic acid and pyrimidine nucleoside antibiotic at the weight ratio of (1:100)-(100:1). The composite can be prepared into a suspending agent and water dispersible granules, has significant sterilization effect and safe to crops.
Description
Technical field the invention belongs to technical field of pesticide, contains specifically the composite bactericide with synergistic effect of Shen piperazine mycin and antibiotic in pyrimidine nucleoside class.
Background technology
Shen piperazine mycin (phenazino-1-carboxylic acid) is a kind of antibiotic through biological culture secretion by pseudomonas fluorescens, there is the dual-use function of wide spectrum Suppressing phytopathogens Promoting plant growth effect simultaneously, this biological bactericide has wide spectrum, feature efficiently, can effectively prevent and treat paddy rice, wheat, fusarium wilt on the crops such as vegetables, climing rot, epidemic disease, banded sclerotial blight, false smut, rice blast, downy mildew, yellow rust, stalk break, head blight, anthracnose, gray mold, scab, leaf spot, bacterial wilt, canker, ginger plague and soil-borne disease soil treatment.
Antibiotic in pyrimidine nucleoside class claims again antimycoin 120, also be 120 agricultural antibiotics (TF120), its bacterial classification Streptomyces ahygroscopicus was separated and obtains from Bei Pu field, the court cotton field root by the Chinese Academy of Agricultural Sciences's clay fertilizer institute's nineteen fifty-seven, antibiotic in pyrimidine nucleoside class be China's independent development a kind of efficient, wide spectrum, strong, the slow resistance of interior suction, pollution-free, noresidue, nuisanceless, toxicity is low, good with Environmental compatibility, with the multi-purpose bactericide of harmony mutually naturally.The disease-resistant spectrum of this antibiotic is wide, after entering in plant corpus, can directly hinder the synthetic of pathogen protein, causes the death of germ, reaches disease-resistant object.It has protection and treatment double action to crop, improves resistance against diseases and the immunocompetence of crop, but its protection, prevention effect are better than treatment, killing action.To powdery mildew, the anthracnose of watermelon, vegetables fusarium wilt, powdery mildew, anthracnose and wheat, Asparagus Stem Blight, rice sheath blight disease, apple canker etc. all have control efficiency.
The agricultural chemicals using mostly at present is synthetic, and the intermediate that has a lot of high poison in synthetic overweight process produces, and the impact that the use of a large amount of chemical pesticides has increasingly sharpened on environment.For current present situation, people start to be more conceived to the more free of contamination biogenic pesticide of low toxic and environment-friendly, because biogenic pesticide is to utilize the antagonist properties between microorganism to prevent and treat pathogen, this is more efficient and environmental protection than existing chemosynthesis composition, new varieties and the complex preparation of exploitation biogenic pesticide are more and more subject to people's attention at present, and two kinds of active ingredients that relate in the present invention are biogenic pesticide.We to strengthening both drug effects, expand fungicidal spectrum by composite to Shen piperazine mycin and antibiotic in pyrimidine nucleoside class, reduce peasant's labour cost.
Summary of the invention
The object of this invention is to provide a kind of bactericidal composition with synergistic effect and safety and environmental protection, realize the object that improves drug effect, reduces pollution, reduces costs.Be specifically related to the bactericidal composition of Shen piperazine mycin and antibiotic in pyrimidine nucleoside class.
Utilize above-mentioned bactericidal composition, be equipped with the known auxiliary agent of those skilled in that art, make formulation of the present invention with the known method of those skilled in that art, comprise water dispersible granules, suspending agent.
In above-mentioned bactericidal composition, the proportion of Shen piperazine mycin and antibiotic in pyrimidine nucleoside class is 1:100-100:1.
Above-mentioned bactericidal composition has following characteristics: 1. biogenic pesticide is little to environmental influence; 2. utilize the antagonism between microorganism to carry out sterilization, effect is strong; 3. formulation environmental protection, does not add organic principle; 4. two kinds of compositions are composite, have synergistic effect; 5. twenty percent divide between without cross resistance; 6. delay the generation of active ingredient resistance, reduce peasant's labour cost.
Embodiment
For determining Shen piperazine mycin and two kinds of composite synergistic effects of active ingredient of antibiotic in pyrimidine nucleoside class, first applicant uses these two kinds of compositions to carry out indoor biometrics test, and test method and result are as follows:
First analyzed after the effect feature of two kinds of active ingredients, the raw object of surveying is decided to be sharp Fusariumsp cucumber specialized form (Fusarium oxysporum (Schl.) F.sp cucumerinum Owen) by we, belongs to Fungi Imperfecti fungi.Aerial hyphae white, fine hair shape, is faint yellow or lavender in PDA medium upper bottom portion.Germ produces two types of conidiums of size, macroconidium spindle or sickleshaped, and water white transparency, terminal cell taper shape, what have is micro-ly hook-shaped, and base portion inverted cone is cut shape or sertoli cell, 1～3, tool barrier film.
The test method of indoor biometrics: adopt growth rate method to measure the inhibitory action of medicament to the growth of rice sheath blight disease mycelia.On the basis of preliminary experiment, from each medicament, germ mycelial growth inhibition rate is reached and within the scope of 10%-90%, designs 5 concentration, the liquid that first the former medicine of 80% antibiotic in pyrimidine nucleoside class and the 70% Shen former medicine of piperazine mycin is made into series concentration take methyl alcohol as solvent is for subsequent use, then by liquid in 1% ratio join melt and the PDA medium of cool to room temperature in, after fully shaking up, pour into and in the culture dish (Φ 90mm) of sterilizing, make band medicine flat board, every processing repeats for four times, to add the blank that is treated to of sterile water; With card punch (Φ 5mm) the picking Rhizoctonia solani Kuhn bacterium cake of sterilizing, mycelia faces down aseptic inoculation in the central authorities with medicine flat board, be inverted in interior cultivation of constant incubator of 25 ℃, after cultivation 6d, adopt right-angled intersection method to measure colony diameter, calculate the EC of each medicament to pathogen
50, and calculate according to the following equation toxicity index, the virulence of more different medicaments and bacteriostasis.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.
The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:
The theoretical toxicity index TTI=∑ (the toxicity index ATI × percentage of this pharmaceutically active ingredient in mixture of certain medicine) of mixture preparation.
It is synergistic effect that co-toxicity coefficient CTC is greater than at 120 o'clock, and being less than at 80 o'clock is antagonism, is addition between 80-120 time.
Indoor biometrics test data sees the following form:
Table 1 Shen piperazine mycin, antibiotic in pyrimidine nucleoside class and the measurement result of different proportioning to cucumber fusarium axysporum co-toxicity coefficient thereof
Analyze above-mentioned data, can obviously find out in the time that Shen piperazine mycin and antibiotic in pyrimidine nucleoside class are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 130, the prevention and control capability of cucumber fusarium axysporum is had to the synergistic effect of different proportion, wherein the synergy between 1:10-10:1 of Shen piperazine mycin and antibiotic in pyrimidine nucleoside class is visible, the composite desirable medicament that becomes control cucumber fusarium axysporum of Shen piperazine mycin and antibiotic in pyrimidine nucleoside class.
Test and determine Shen piperazine mycin and two kinds of active ingredients of antibiotic in pyrimidine nucleoside class are composite can good synergistic effect by indoor biometrics, below by instantiation in order to further describe the present invention, but the present invention absolutely not only only limits to following these embodiment.In formula, percentage is all weight percentage, and the former medicine using and auxiliary agent are other producers and buy.
Formulation examples is as follows:
Example one, 10.1% Shen piperazine mycin antibiotic in pyrimidine nucleoside class suspending agent
Example two, 20.4% Shen piperazine mycin antibiotic in pyrimidine nucleoside class suspending agent
Example three, 15.5% Shen piperazine mycin antibiotic in pyrimidine nucleoside class suspending agent
Example four, 8.8% Shen piperazine mycin antibiotic in pyrimidine nucleoside class suspending agent
Example five, 6% Shen piperazine mycin antibiotic in pyrimidine nucleoside class suspending agent
Example six, 3% Shen piperazine mycin antibiotic in pyrimidine nucleoside class water dispersible granules
Example seven, 3.3% Shen piperazine mycin antibiotic in pyrimidine nucleoside class water dispersible granules
Example eight, 30.6% Shen piperazine mycin antibiotic in pyrimidine nucleoside class water dispersible granules
Example nine, 20.2% Shen piperazine mycin antibiotic in pyrimidine nucleoside class water dispersible granules
Select above example to prevent and treat the field trial of cucumber fusarium axysporum, determine the effect of formula control cucumber fusarium axysporum; Simultaneously by with the Contrast on effect of Shen piperazine mycin and antibiotic in pyrimidine nucleoside class single dose, verify the synergistic effect after composite.Concrete test method data are as follows:
6% Shen piperazine mycin antibiotic in pyrimidine nucleoside class suspending agent and other preparation do the test of pesticide effectiveness of field control cucumber fusarium axysporum.Test site is Weifang City of Shandong Province, time is on May 20th, 2013, test three repetitions of each processing, community randomized arrangement, each community area is 30m2, adopts back-pack electric compression sprayer, dispenser was sunny calm the same day, the planting habit of all communities is all identical with local planting habit, records the dispenser same day and the synoptic data of a week thereafter, specific as follows:
Table 2 duration of test meteorological data
Investigation method is: each community adopts five point samplings, and each point is got five strain cucumber seedlings, and after dispenser, fixed point investigation in the 7th day, 14 days, investigates each community disease and refer to, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.
Cucumber fusarium axysporum severity Scaling standard, with reference to methods such as Huang Yao armies:
0 grade: growth is normal
L level: blade or stem wilting area from bottom to top account for 1/4 of complete stool
Or less than 1/4
2 grades: blade or stem wilting area from bottom to top account for 1/2 of complete stool
3 grades: blade or stem wilting area from bottom to top account for 3/4 of complete stool
4 grades: the leaf of diseased plant is all wilted or whole strain death
Table 36% Shen piperazine mycin antibiotic in pyrimidine nucleoside class suspending agent and the preventive effect of different medicament to rice sheath blight disease
From above-mentioned data, can find out, Shen piperazine mycin and antibiotic in pyrimidine nucleoside class complex preparation 7 days preventive effects after medicine all exceed 75%, and piperazine mycin single dose 7 days preventive effects after medicine in Shen are 50.32%, the preventive effect of antibiotic in pyrimidine nucleoside class single dose is 47.11%, compare Shen piperazine mycin and antibiotic in pyrimidine nucleoside class single dose, between complex preparation and single dose, have the preventive effect of utmost point significant difference, visible two active ingredients are composite good synergistic effect.And after medicine 14 days, all complex preparations have all exceeded 85% to the preventive effect of cucumber fusarium axysporum, and the lasting period of composite rear preparation extends, more lasting to the control efficiency of cucumber fusarium axysporum.Complex preparation can well improve drug effect, extends the lasting period, alleviates peasant's the amount of labour, has very high practicality.
Claims (3)
1. a bactericidal composition, is characterized in that: active component is made up of Shen piperazine mycin and antibiotic in pyrimidine nucleoside class, and the weight ratio of described Shen piperazine mycin and antibiotic in pyrimidine nucleoside class is 1:100～100:1.
2. bactericidal composition according to claim 1, is characterized in that: make suspending agent, water dispersible granules by active ingredient and auxiliary material.
3. according to claim 1 or bactericidal composition claimed in claim 2, it is characterized in that: for preventing and treating cucumber fusarium axysporum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410168470.9A CN103907644A (en) | 2014-04-24 | 2014-04-24 | Sterilization composite |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410168470.9A CN103907644A (en) | 2014-04-24 | 2014-04-24 | Sterilization composite |
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| Publication Number | Publication Date |
|---|---|
| CN103907644A true CN103907644A (en) | 2014-07-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410168470.9A Pending CN103907644A (en) | 2014-04-24 | 2014-04-24 | Sterilization composite |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186551A (en) * | 2014-09-25 | 2014-12-10 | 江苏省绿盾植保农药实验有限公司 | Pyrimidine nucleoside antibiotics and shenqinmycin biological bactericidal composition and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08259409A (en) * | 1995-03-20 | 1996-10-08 | Sumitomo Forestry Co Ltd | Snow blight controlling material and controlling method |
| CN103155925A (en) * | 2011-12-18 | 2013-06-19 | 深圳诺普信农化股份有限公司 | Sterilization composite containing phenazino-1-carboxylic acid |
| CN103271094A (en) * | 2013-06-19 | 2013-09-04 | 北京燕化永乐农药有限公司 | Compound bactericide |
-
2014
- 2014-04-24 CN CN201410168470.9A patent/CN103907644A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08259409A (en) * | 1995-03-20 | 1996-10-08 | Sumitomo Forestry Co Ltd | Snow blight controlling material and controlling method |
| CN103155925A (en) * | 2011-12-18 | 2013-06-19 | 深圳诺普信农化股份有限公司 | Sterilization composite containing phenazino-1-carboxylic acid |
| CN103271094A (en) * | 2013-06-19 | 2013-09-04 | 北京燕化永乐农药有限公司 | Compound bactericide |
Non-Patent Citations (2)
| Title |
|---|
| 无: "茄子黄萎病害防治-图文识别技术", 《无》 * |
| 陈俊伟: "供应杀虫剂、杀菌剂", 《无》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186551A (en) * | 2014-09-25 | 2014-12-10 | 江苏省绿盾植保农药实验有限公司 | Pyrimidine nucleoside antibiotics and shenqinmycin biological bactericidal composition and application thereof |
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Application publication date: 20140709 |