Background technology
Natamycin also claims pimaricin, natamycin, a kind of two sixteen-ring polyene macrolide antifungal agent, primarily of streptomyces chatanoogensis, Streptomyces natalensis and brown yellow spore streptomycete etc. through fermentation, extraction, refining a kind of secondary metabolite, the bacteriostasis of natamycin to fungi is extremely strong.Can efficiently, the antifungi of wide spectrum, saccharomycete, some protozoa and some algae agent, can interact with cutter alcoholic compound and there is the compatibility of height, inhibit activities is had to fungi, its Antibacterial Mechanism is that it can react with the sterol compounds on cell membrane, trigger cell membrane structure changes and breaks thus, make the seepage of cellular content, cause cell death.Natamycin is fine for the living cells effect suppressing breeding, and then needs higher concentration, meanwhile, also have certain inhibitory action to fungal spore for destruction rest cell.
Shen piperazine mycin (phenazino-1-carboxylic acid) is a kind of antibiotic secreted through biological culture by pseudomonas fluorescens, there is wide spectrum Suppressing phytopathogens simultaneously and the dual-use function of Promoting plant growth effect, this biological bactericide has wide spectrum, efficient feature, effectively can prevent and treat paddy rice, wheat, fusarium wilt on the crops such as vegetables, blight dis-ease, epidemic disease, banded sclerotial blight, false smut, rice blast, downy mildew, yellow rust, stalk break, head blight, anthracnose, gray mold, scab, leaf spot, bacterial wilt, canker, ginger plague and soil-borne disease soil treatment.
Rice sheath blight disease (Rhizoctorzia solani), also known as moire disease, is infected by Rhizoctonia solani Kuhn and fall ill, and how to fall ill under high temperature, super-humid conditions.Banded sclerotial blight is serious in the harm of south rice region, is one of Major Diseases in current Rice Production.This disease can make paddy rice not ear, and particle weight declines, and has a strong impact on rice yield.
The agricultural chemicals of current use mostly is synthetic, and the intermediate having a lot of high poison in the overweight process of synthesis produces, and the use of a large amount of chemical pesticide has increasingly sharpened on the impact of environment.For current present situation, people start more to be conceived to the free of contamination biogenic pesticide of more low toxic and environment-friendly, because biogenic pesticide utilizes the antagonist properties between microorganism to prevent and treat pathogen, this is more efficient and environmental protection than existing chemosynthesis composition, new varieties and the complex preparation of current exploitation biogenic pesticide are more and more subject to people's attention, and relate in the present invention two kinds of active ingredients are biogenic pesticide.We by natamycin and Shen piperazine mycin composite, to the drug effect both strengthening, expand fungicidal spectrum, reduce the labour cost of peasant.
Embodiment
For determining natamycin and the composite synergistic effect of Shen piperazine mycin two kinds of active ingredients, first applicant uses these two kinds of compositions to carry out indoor biometrics test, test method and result as follows:
First after analyzing the action character of two kinds of active ingredients, the raw object surveyed is decided to be thanatephorus cucumeris(frank) donk (Thanatephorus cucumeris), this Pseudomonas Basidiomycotina fungi by us, and sclerotium is dark brown, bacterium colony is light brown to taupe mildew, and this bacterium can cause the banded sclerotial blight of paddy rice.
The test method of indoor biometrics: adopt the inhibitory action that growth rate method mensuration medicament grows rice sheath blight disease mycelia.On the basis of preliminary experiment, from each medicament, design 5 concentration within the scope of 10%-90% are reached to germ mycelial growth inhibition rate, it is for subsequent use by the former medicine of 95% natamycin and the 95% Shen former medicine of piperazine mycin with methyl alcohol to be first that solvent is made into the liquid of series concentration, then liquid is joined in 1% ratio in the PDA medium melting also cool to room temperature, after fully shaking up, pour in the culture dish (Φ 90mm) of sterilizing and make band medicine flat board, often process and repeat, to add the process of sterile water for blank for four times; With card punch (Φ 5mm) the picking Rhizoctonia solani Kuhn bacterium cake of sterilizing, mycelia faces down aseptic inoculation in the central authorities of band medicine flat board, be inverted in the interior cultivation of constant incubator of 25 DEG C, adopt right-angled intersection method to measure colony diameter after cultivating 6d, calculate the EC of each medicament to pathogen
50, and calculate toxicity index according to the following equation, compare virulence and the bacteriostasis of different agents.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.
The co-toxicity coefficient method representation that the co-toxicity of mixture preparation adopts Sun Yun abundant:
The theoretical toxicity index TTI=∑ of mixture preparation (percentage of this pharmaceutically active ingredient of the toxicity index ATI of certain medicine × in mixture).
Be synergistic effect when co-toxicity coefficient CTC is greater than 120, being antagonism when being less than 80, is addition time between 80-120.
Indoor biometrics test data sees the following form:
Table 1 natamycin, Shen piperazine mycin and different ratio thereof are to the measurement result of rice sheath blight disease co-toxicity coefficient
Analyze above-mentioned data, can obviously find out when natamycin and Shen piperazine mycin are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 120, the prevention and control capability of rice sheath blight disease is had to the synergistic effect of different proportion, wherein the synergy between 1:5-5:1 namely between proportioning five to proportioning seven of natamycin and Shen piperazine mycin is visible.
Determine natamycin by indoor biometrics test and piperazine mycin two kinds of active ingredients in Shen are composite can good synergistic effect, below by way of instantiation in order to further describe the present invention, but the present invention is absolutely not only only limitted to these embodiments following.In formula, percentage is all weight percentage, and the former medicine used and auxiliary agent are other producers and buy.
Formulation examples is as follows:
Example one, 10.1% natamycin Shen piperazine mycin suspending agent
Example two, 21% natamycin Shen piperazine mycin water dispersible granules
Example three, 5.5% natamycin Shen piperazine mycin suspending agent
Example four, 25.5% natamycin Shen piperazine mycin water dispersible granules
Example five, 6% natamycin Shen piperazine mycin suspending agent
Example six, 4% natamycin Shen piperazine mycin water dispersible granules
Example seven, 6.6% natamycin Shen piperazine mycin suspending agent
Example eight, 30.6% natamycin Shen piperazine mycin water dispersible granules
Example nine, 20.2% natamycin Shen piperazine mycin suspending agent
Example ten, 15% natamycin Shen piperazine mycin water dispersible granules
Select above example to carry out the field trial of the water prevention rice sheath blight, determine the effect of the formula water prevention rice sheath blight; Simultaneously by the Contrast on effect with natamycin and Shen piperazine mycin single dose, verify composite after synergistic effect.Concrete test method data are as follows:
6% natamycin Shen piperazine mycin suspending agent and other preparation do the test of pesticide effectiveness of field control rice sheath blight disease.Test site is Hanchuan City, Hubei Province, and the time is on June 13rd, 2012, and test each process three repetition, community randomized arrangement, each plot area is 70m
2, adopt back-pack electric compression sprayer, dispenser was sunny calm for the same day, and the planting habit of planting habit all with local of all communities is identical, records the synoptic data on the dispenser same day and a week thereafter, specific as follows:
Table 2 duration of test meteorological data
Investigation method is: fixed 2 points of each community diagonal, and often some straight ahead determines 25 clumps, and after dispenser, fixed point investigation in the 7th day, 21 days, investigates each community disease and refer to, calculate preventive effect, and carry out the new multipole difference analysis of Deng Kenshi.
The grade scale of disease index:
0 grade: without scab;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for 6% ~ 10% of whole leaf area;
5 grades: lesion area accounts for 11% ~ 25% of whole leaf area;
7 grades: lesion area accounts for 26% ~ 50% of whole leaf area;
9 grades: lesion area accounts for more than 51% of whole leaf area.
Table 36% natamycin Shen piperazine mycin suspending agent and different agents are to the preventive effect of rice sheath blight disease
As can be seen from above-mentioned data, natamycin and Shen piperazine mycin complex preparation after medicine 7 days preventive effects all more than 75%, and natamycin single dose 7 days preventive effects after medicine are 46.21%, the preventive effect of Shen piperazine mycin single dose is 42.30%, compare natamycin and Shen piperazine mycin single dose, have the preventive effect of pole significant difference between complex preparation and single dose, visible two active ingredients are composite good synergistic effect.And after medicine 21 days, all complex preparations have all exceeded 85% to the preventive effect of rice sheath blight disease, and the lasting period of composite rear preparation extends, more lasting to the control efficiency of rice sheath blight disease.Complex preparation can well improve drug effect, extends the lasting period, alleviates the amount of labour of peasant, have very high practicality.