Technical field the invention belongs to technical field of pesticide, contains specifically the composite bactericide with synergistic effect of natamycin and Shen piperazine mycin.
Background technology
Natamycin also claims pimaricin, natamycin, a kind of two sixteen-ring polyene macrolide antifungal agent, mainly by streptomyces chatanoogensis, Natal streptomycete and brown yellow spore streptomycete etc. through fermentation, extract, the refining a kind of secondary metabolite forming, natamycin is extremely strong to the bacteriostasis of fungi.Can be efficiently, antifungi, saccharomycete, some protozoa and some algae agent of wide spectrum, can interact and have the compatibility of height with cutter alcoholic compound, fungi is had and suppresses active, its Antibacterial Mechanism is that it can react with the sterol compounds on cell membrane, trigger cell membrane structure changes and breaks thus, the seepage that makes cellular content, causes cell death.Natamycin is fine for the living cells effect that suppresses breeding, and for destroying rest cell, needs higher concentration, meanwhile, fungal spore is also had to certain inhibitory action.
Shen piperazine mycin (phenazino-1-carboxylic acid) is a kind of antibiotic through biological culture secretion by pseudomonas fluorescens, the dual-use function simultaneously with wide spectrum Suppressing phytopathogens Promoting plant growth effect, this biological bactericide has wide spectrum, efficient feature, can effectively prevent and treat paddy rice, wheat, fusarium wilt on the crops such as vegetables, climing rot, epidemic disease, banded sclerotial blight, false smut, rice blast, downy mildew, yellow rust, stalk break, head blight, anthracnose, gray mold, scab, leaf spot, bacterial wilt, canker, ginger plague and soil-borne disease soil treatment.
Rice sheath blight disease (Rhizoctorzia solani) claim again moire disease, is infected and fallen ill by Rhizoctonia solani Kuhn, how under high temperature, super-humid conditions, to fall ill.Banded sclerotial blight is serious in the harm of south rice region, is one of Major Diseases in current Rice Production.This disease can make paddy rice not ear, and particle weight declines, and has a strong impact on rice yield.
The agricultural chemicals using mostly at present is synthetic, and the intermediate that has a lot of high poison in synthetic overweight process produces, and the impact that the use of a large amount of chemical pesticides has increasingly sharpened on environment.For current present situation, people start to be more conceived to the more free of contamination biogenic pesticide of low toxic and environment-friendly, because biogenic pesticide is to utilize the antagonist properties between microorganism to prevent and treat pathogen, this is more efficient and environmental protection than existing chemosynthesis composition, new varieties and the complex preparation of exploitation biogenic pesticide are more and more subject to people's attention at present, and two kinds of active ingredients that relate in the present invention are biogenic pesticide.We are composite by natamycin and Shen piperazine mycin, to strengthening both drug effects, expand fungicidal spectrum, reduce peasant's labour cost.
Embodiment
For determining natamycin and two kinds of composite synergistic effects of active ingredient of Shen piperazine mycin, first applicant uses these two kinds of compositions to carry out indoor biometrics test, and test method and result are as follows:
First analyzed after the effect feature of two kinds of active ingredients, we are decided to be thanatephorus cucumeris(frank) donk (Thanatephorus cucumeris) by the raw object of surveying, this Pseudomonas Basidiomycotina fungi, and sclerotium is dark brown, it is light brown to taupe mildew that bacterium colony is, and this bacterium can cause the banded sclerotial blight of paddy rice.
The test method of indoor biometrics: adopt growth rate method to measure the inhibitory action of medicament to the growth of rice sheath blight disease mycelia.On the basis of preliminary experiment, from each medicament, germ mycelial growth inhibition rate is reached and within the scope of 10%-90%, designs 5 concentration, first the former medicine of 95% natamycin and the 95% Shen former medicine of piperazine mycin be take methyl alcohol as the liquid that solvent is made into series concentration standby, then by liquid in 1% ratio join melt and the PDA medium of cool to room temperature in, after fully shaking up, pour that in the culture dish (Φ 90mm) of sterilizing, to make band medicine dull and stereotyped into, every processing repeats for four times, to add the blank that is treated to of sterile water; Card punch (Φ 5mm) picking Rhizoctonia solani Kuhn bacterium cake with sterilizing, mycelia faces down aseptic inoculation in the central authorities with medicine flat board, be inverted in interior cultivation of constant incubator of 25 ℃, after cultivation 6d, adopt right-angled intersection method to measure colony diameter, calculate the EC of each medicament to pathogen
50, and calculate according to the following equation toxicity index, the virulence of more different medicaments and bacteriostasis.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.
The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:
The theoretical toxicity index TTI=∑ of mixture preparation (the toxicity index ATI of certain medicine * percentage of this pharmaceutically active ingredient in mixture).
It is synergistic effect that co-toxicity coefficient CTC is greater than at 120 o'clock, and being less than at 80 o'clock is antagonism, is addition in the time of between 80-120.
Indoor biometrics test data sees the following form:
Table 1 natamycin, Shen piperazine mycin and the measurement result of different proportioning to rice sheath blight disease co-toxicity coefficient thereof
Analyze above-mentioned data, can obviously find out when natamycin and Shen piperazine mycin are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 120, the prevention and control capability of rice sheath blight disease is had to the synergistic effect of different proportion, wherein natamycin and Shen piperazine mycin are being that proportioning five is visible to the synergy between proportioning seven between 1:5-5:1.
By indoor biometrics, test and determine natamycin and piperazine mycin two kinds of active ingredients in Shen are composite can good synergistic effect, below by instantiation in order to further describe the present invention, but the present invention absolutely not only only limits to following these embodiment.In formula, percentage is all weight percentage, and the former medicine using and auxiliary agent are other producers and buy.
Formulation examples is as follows:
Example one, 10.1% natamycin Shen piperazine mycin suspending agent
Example two, 21% natamycin Shen piperazine mycin water dispersible granules
Example three, 5.5% natamycin Shen piperazine mycin suspending agent
Example four, 25.5% natamycin Shen piperazine mycin water dispersible granules
Example five, 6% natamycin Shen piperazine mycin suspending agent
Example six, 4% natamycin Shen piperazine mycin water dispersible granules
Example seven, 6.6% natamycin Shen piperazine mycin suspending agent
Example eight, 30.6% natamycin Shen piperazine mycin water dispersible granules
Example nine, 20.2% natamycin Shen piperazine mycin suspending agent
Example ten, 15% natamycin Shen piperazine mycin water dispersible granules
Select above example to prevent and treat the field trial of rice sheath blight disease, the formula of determining is prevented and treated the effect of rice sheath blight disease; Simultaneously by the Contrast on effect with natamycin and Shen piperazine mycin single dose, verify the synergistic effect after composite.Concrete test method data are as follows:
6% natamycin Shen piperazine mycin suspending agent and other preparation are done the test of pesticide effectiveness of field control rice sheath blight disease.Test site is Hanchuan City, Hubei Province, and the time is on June 13rd, 2012, test each and process three repetitions, and community randomized arrangement, each community area is 70m
2, adopting back-pack electric compression sprayer, dispenser was sunny calm the same day, and the planting habit of all communities is all identical with local planting habit, records the dispenser same day and the synoptic data of a week thereafter, specific as follows:
Table 2 duration of test meteorological data
Investigation method is: fixed 2 points of each community diagonal, and every some straight ahead is determined 25 clumps, and after dispenser, fixed point investigation in the 7th day, 21 days, investigates each community disease and refers to, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.
The grade scale of disease index:
0 grade: without scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%~10% of whole leaf area;
5 grades: lesion area accounts for 11%~25% of whole leaf area;
7 grades: lesion area accounts for 26%~50% of whole leaf area;
9 grades: lesion area accounts for the more than 51% of whole leaf area.
Table 36% natamycin Shen piperazine mycin suspending agent and the preventive effect of different medicament to rice sheath blight disease
From above-mentioned data, can find out, natamycin and Shen piperazine mycin complex preparation after medicine 7 days preventive effects all over 75%, and natamycin single dose 7 days preventive effects after medicine are 46.21%, the preventive effect of Shen piperazine mycin single dose is 42.30%, compare natamycin and Shen piperazine mycin single dose, the preventive effect that has utmost point significant difference between complex preparation and single dose, visible two active ingredients are composite good synergistic effect.And after medicine 21 days, all complex preparations have all surpassed 85% to the preventive effect of rice sheath blight disease, and the lasting period of composite rear preparation extends, more lasting to the control efficiency of rice sheath blight disease.Complex preparation can well improve drug effect, extends the lasting period, alleviates peasant's the amount of labour, has very high practicality.