CN102812956A - Bactericidal composition - Google Patents
Bactericidal composition Download PDFInfo
- Publication number
- CN102812956A CN102812956A CN2012103482416A CN201210348241A CN102812956A CN 102812956 A CN102812956 A CN 102812956A CN 2012103482416 A CN2012103482416 A CN 2012103482416A CN 201210348241 A CN201210348241 A CN 201210348241A CN 102812956 A CN102812956 A CN 102812956A
- Authority
- CN
- China
- Prior art keywords
- kasugarnycin
- bactericidal composition
- piperazine mycin
- rice
- agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a bactericidal composition. The bactericidal composition contains two active ingredients, namely shenqinmycin and kasugamycin. The bactericidal composition is low-toxin and efficient, has an enlarged bactericidal spectrum, and can be used for reducing the consumption, reducing the labor cost of peasants, delaying agent resistance, prolonging the lasting period, and reducing environmental pollution.
Description
Technical field
The invention belongs to technical field of pesticide, specifically a kind of bactericidal composition that contains Shen piperazine mycin and kasugarnycin.
Background technology
Shen piperazine mycin (phenazino-1-carboxylic acid) is by a kind of antibiotic of pseudomonas fluorescens through the biological culture secretion; Has the dual-use function that wide spectrum suppresses phytopathogen and promotes the plant growing effect simultaneously; This biological bactericide has wide spectrum, characteristics of high efficiency, can effectively prevent and treat fusarium wilt, climing rot, eqpidemic disease, banded sclerotial blight, false smut, rice blast, downy mildew, yellow rust, stalk break, head blight, anthracnose, gray mold, scab, leaf spot, bacterial wilt, canker, ginger plague and the soil-borne disease soil treatment on the crops such as paddy rice, wheat, vegetables.
Kasugarnycin (kasugamycin) is the metabolite that Actinomyces microaureus produces, and not only fungi is had activity, and bacteriosis is also had control efficiency, has stronger interior absorption.Its mechanism of action is to disturb the esterase system of metabolism of amino acid, thereby influences the synthetic of protein, suppresses the mycelia elongation and causes cell granulationsization.Kasugarnycin is the disinfectant use in agriculture of high-efficiency low-toxicity, and the rice blast on the paddy rice is had excellent preventive effect and therapeutic action.Control watermelon bacterial angular leaf spot, peach gummosis, shot hole, diseases such as shothole disease have special efficacy.
Along with a large amount of uses of agricultural chemicals, pesticide hazards problem and people become increasingly conspicuous to the concern of living environment.To the influence of present agricultural chemicals to environment, people begin more to be conceived to the more free of contamination biogenic pesticide of low toxic and environment-friendly, the new varieties and the complex preparation of exploitation biogenic pesticide.The field efficacy experiment shows, the existing medicament another kind of mixed pesticide different with mechanism of action can not only be postponed resistance and produced, and can also improve drug effect and residual period, reduces usage amount, reduces peasant's dispenser cost.Therefore we are composite with Shen piperazine mycin and kasugarnycin, in order to enlarge fungicidal spectrum, reduce peasant's labour cost.
Summary of the invention
The purpose of this invention is to provide a kind of bactericidal composition, realize reducing toxicity, reduce the dispenser cost that pollutes, reduces the peasant, increase economic efficiency with synergistic effect and safety and environmental protection.Concrete Shen piperazine mycin and the composite bactericidal composition of kasugarnycin of relating to.
Utilize the described bactericidal composition of the invention described above, be equipped with the known auxiliary agent of those skilled in that art, with those skilled in that art's known method, the formulation of its processing is suspending agent, wetting powder, water dispersible granules.
In the above-mentioned bactericidal composition, contain Shen piperazine mycin 0.1%～10%, kasugarnycin 0.1%～15%.
Advantage and excellent effect that Shen piperazine mycin and kasugarnycin bactericidal composition have: 1. bactericidal range is wide, and is good to paddy rice, wheat, oranges and tangerines, cotton, cucumber, banana, watermelon, capsicum control efficiency; 2. adhesive force improves, and penetrability strengthens; 3. under low-down dosage, have good effect, and two kinds of former medicines there is not cross resistance; 4. use compound formulation, consumption reduces during all than single use, and stability increases, and formulation is advanced, environmental protection, and is safe.
Embodiment
For confirming Shen piperazine mycin and two kinds of composite synergistic effects of active ingredient of kasugarnycin, the applicant at first uses these two kinds of compositions to carry out the indoor biometrics experiment, and experimental technique and result are following:
According to the characteristic of product active ingredient and the demand in market, the object that we will give birth to survey is decided to be Rhizoctonia solani Kuhn (Thanatephorus cucumeris).Rice sheath blight disease is claimed moire disease again, and seedling stage to the fringe phase all can fall ill, and is one of important disease of generally taking place of paddy rice producing region in the world.China has fallen ill in rising trend since the seventies in each rice district.According to statistics, present national onset area reaches more than 200,000,000 mu (account for the gross area 2/5), constitutes the three big diseases of paddy rice with rice blast, bacterial leaf-blight.Banded sclerotial blight can make that plants stems stalk, leaf sheath are withered to cause that to rotting ripening rate descends, and thousand kernel weight reduces, even plant lodging and having no harvest.In recent years because paddy rice continuous cropping area constantly enlarges and the increasing of planting density; Make the rice blast that is only second to of this disease; The loss that causes also increases year by year; Medicament commonly used is a jinggangmeisu on the market now, but the lasting period of jinggangmeisu is short, and the use of adding for many years descends the effect of jinggangmeisu.Therefore, be badly in need of on the market rice sheath blight disease special efficacy, long medicament of lasting period.
The test method of indoor biometrics: adopt growth rate method to measure the inhibitory action of medicament to the growth of rice sheath blight disease mycelia.On the basis of preliminary experiment; From each medicament the germ mycelial growth inhibition rate is reached in the 10%-90% scope 5 concentration of design, it is subsequent use earlier the 96% former medicine of Shen piperazine mycin and the former medicine of 95% kasugarnycin with methyl alcohol to be that solvent is made into the soup of series concentration, joins soup in the PDA medium that has melted in 1% ratio then; After fully shaking up; Pour that to process the band poison in the culture dish (Φ 90mm) of sterilization dull and stereotyped into, every processing repeats for four times, to add the blank that is treated to of 1% methyl alcohol; Card punch (Φ 5mm) picking Rhizoctonia solani Kuhn bacterium cake with sterilization; Mycelia faces down aseptic inoculation in the dull and stereotyped central authorities of band poison; Place 25 ℃ interior cultivation of constant incubator, adopt the right-angled intersection method to measure colony diameter behind the cultivation 6d, calculate the EC of each medicament pathogen
50, the virulence of more different medicaments and bacteriostasis, above-mentioned living survey used former medicine of experiment and reagent are all bought from other producers.
The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:
The theoretical toxicity index A=∑ of mixture preparation (toxicity index of certain medicine * percentage of this pharmaceutically active ingredient in mixture)
C was synergistic effect greater than 120 o'clock, less than 80 o'clock be antagonism, 80～120 o'clock is addition.
The indoor biometrics experimental data sees the following form:
Table 1 Shen piperazine mycin, kasugarnycin and different proportioning thereof are to the mensuration result of rice sheath blight disease co-toxicity coefficient
Analyze above-mentioned data, can obviously find out when Shen piperazine mycin and kasugarnycin different proportion are composite rice sheath blight disease is all had the synergistic effect of different proportion; The co-toxicity coefficient (CTC) of prescription is all greater than 120; Desirable synergistic effect is arranged, and wherein the co-toxicity coefficient of proportioning five is maximum, has reached 181.20; Secondly be proportioning four, co-toxicity coefficient reaches 175.31.Therefore, the composite desirable medicament of preventing and treating rice sheath blight disease that becomes of Shen piperazine mycin and kasugarnycin.
Confirm that through indoor biometrics experiment Shen piperazine mycin can have good synergistic effect with two kinds of active ingredients of kasugarnycin are composite, below pass through instantiation in order to further explain the present invention, but the present invention absolutely not only is limited to following these embodiment.Percentage all is weight percentage in the prescription, and employed former medicine and auxiliary agent are other producers and buy.
Formulation examples is following:
One: 2% Shen of instance piperazine mycin kasugarnycin suspending agent
According to the prescription requirement, be medium with water, with grinding in the adding sand mills such as active ingredient, wetting agent, dispersant, thickener, antifoaming agent, the back adds emulsification in the high-shear emulsifying still, makes suspending agent.
Two: 15.1% Shens of instance piperazine mycin kasugarnycin wetting powder
Active ingredient, wetting agent, dispersant, filler etc. are fully mixed in the ratio of prescription, and after ultrafine crusher was pulverized, fineness reached 98% through 325 mesh standard sieves, promptly gets wetting powder.
Three: 3% Shens of instance piperazine mycin kasugarnycin suspending agent
According to the prescription requirement, be medium with water, with grinding in the adding sand mills such as active ingredient, wetting agent, dispersant, thickener, antifoaming agent, the back adds emulsification in the high-shear emulsifying still, makes suspending agent.
Four: 8% Shens of instance piperazine mycin kasugamycin water-dispersion granule
Active ingredient, wetting agent, dispersant, adhesive, filler etc. are fully mixed in the ratio of prescription, after ultrafine crusher is pulverized, add an amount of purified water, make water dispersible granules through kneading, granulation, drying, screening.
Five: 10.1% Shens of instance piperazine mycin kasugarnycin wetting powder
Active ingredient, wetting agent, dispersant, filler etc. are fully mixed in the ratio of prescription, and after ultrafine crusher was pulverized, fineness reached 98% through 325 mesh standard sieves, promptly gets wetting powder.
Six: 12% Shens of instance piperazine mycin kasugarnycin wetting powder
Active ingredient, wetting agent, dispersant, filler etc. are fully mixed in the ratio of prescription, and after ultrafine crusher was pulverized, fineness reached 98% through 325 mesh standard sieves, promptly gets wetting powder.
Select above instance to prevent and treat the field trial of rice sheath blight disease, the prescription of confirming is prevented and treated the effect of rice sheath blight disease; Simultaneously through with the effect comparison of Shen piperazine mycin, the agent of kasugarnycin list, verify the synergistic effect after composite.Concrete test method data are following:
Test one: 2% Shen piperazine mycin kasugarnycin suspending agent and other preparation and do the test of pesticide effectiveness of field control rice sheath blight disease.The test place is Huaihua City Zhi Jiang county, Hunan Province, and the time is on June 15th, 2011, test totally five processing; Each handles three repetitions, and the sub-district randomized arrangement adopts the back-pack electric compression sprayer; There is shallow water 3-4cm in the field during dispenser, and dispenser was sunny calm the same day, and the planting habit of all sub-districts all planting habit with local is identical; Record dispenser same day and all synoptic data thereafter, specific as follows:
Table 2 duration of test meteorological data
Time: in June, 2011 place: Hunan Zhi Jiang
Investigation method is: fixed 2 points of each sub-district diagonal, fixed 25 clumps of every some straight ahead, after the dispenser the 7th day, 14 days,
Fixed point investigation in 21 days is investigated each sub-district disease and is referred to, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.
The grade scale of disease index:
0 grade of complete stool is anosis
1 grade of quaterfoil and following each leaf sheath thereof, blade morbidity (is first blade with sword-like leave)
3 grade of the 3rd blade and following each leaf sheath thereof, blade morbidity
5 grade of second blade and following each leaf sheath thereof, blade morbidity
7 grades of sword-like leave blades and following each leaf sheath thereof, blade morbidity
9 grades of complete stool morbidities, withered ahead of time
Table 3 2% Shen piperazine mycin kasugarnycin suspending agent and different medicament are to the preventive effect of rice sheath blight disease
Annotate: data are the mean value that repeats for 3 times in the table, and letter is all Duncan testing differentia remarkable time mutually: in June, 2011 place; Hunan Zhi Jiang
From above-mentioned data, can find out; Shen piperazine mycin and kasugarnycin complex preparation behind medicine 7 days preventive effects all above 60%; And 5% kasugarnycin list agent, 7 days preventive effects behind medicine are 27.66%; 5% Shen piperazine mycin list agent is 40.36%, compares Shen piperazine mycin and the agent of kasugarnycin list, and the preventive effect of complex preparation and single agent all have extremely significant difference.And behind medicine 21 days, preventive effect reaches 92.46%, far above single agent of Shen piperazine mycin list agent and kasugarnycin, good synergistic effect is arranged.Simultaneously the complex preparation of Shen piperazine mycin and kasugarnycin preventive effect of 14 days, 21 days behind medicine also is higher than single agent of Shen piperazine mycin and kasugarnycin far away.
Through above-mentioned field control effectiveness test, confirm that the sheath blight disease controlling effect of a pair of paddy rice of instance is the most desirable.Therefore, measuring and calculating sub-district real grain number, thousand kernel weight, actual production are calculated sterile grain rate and effect of increasing production when paddy rice harvesting, and also whether with the significance of Deng Kenshi duncan's new multiple range method check rice yield otherness, observing instance one has production-increasing function to rice yield.
Table 4 2% Shen piperazine mycin kasugarnycin suspending agent and different medicament are to the influence of rice yield
Annotate: data are the mean value that repeats for 3 times in the table, and letter is all Duncan testing differentia remarkable time mutually: in June, 2011 place: Hunan Zhi Jiang
Can find out that from above-mentioned data use-case one prescription has significant production-increasing function to paddy rice, sterile grain rate is starkly lower than other two medicaments, and thousand kernel weight also has tangible increase.The field efficacy experiment is observed and is found the plot of use-case one, strong, the blue or green bar yellow maturity of paddy rice individuality, full grains.Therefore, instance one has the effect of tangible raising the output and raising rice quality.
Can obviously find out from the field trial data, the effect the when preventive effect of complex preparation and production-increasing function all use greater than Shen piperazine mycin, the agent of kasugarnycin list, and also the lasting period after composite is about 30 days, also greater than lasting period of single agent.Two kinds of active ingredient is composite can to increase the lasting period, improves drug effect, and very high economic benefit is arranged.
Claims (2)
1. a bactericidal composition is characterized in that: contain two kinds of active components of Shen piperazine mycin and kasugarnycin.
2. bactericidal composition according to claim 1 is characterized in that: piperazine mould cellulose content weight in Shen is 0.1%-10%, and kasugarnycin content weight is 0.1%-15%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210348241 CN102812956B (en) | 2012-09-18 | 2012-09-18 | Bactericidal composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210348241 CN102812956B (en) | 2012-09-18 | 2012-09-18 | Bactericidal composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102812956A true CN102812956A (en) | 2012-12-12 |
| CN102812956B CN102812956B (en) | 2013-08-14 |
Family
ID=47297671
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210348241 Active CN102812956B (en) | 2012-09-18 | 2012-09-18 | Bactericidal composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102812956B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103271094A (en) * | 2013-06-19 | 2013-09-04 | 北京燕化永乐农药有限公司 | Compound bactericide |
| CN103444721A (en) * | 2013-09-05 | 2013-12-18 | 肇庆长龙生物科技有限公司 | Sterilization pesticide composition, pesticide preparation and application of pesticide preparation |
| CN103651430A (en) * | 2013-12-17 | 2014-03-26 | 北京燕化永乐生物科技股份有限公司 | Compound fungicide |
| CN103960245A (en) * | 2011-12-18 | 2014-08-06 | 深圳诺普信农化股份有限公司 | Sterilization composition containing phenazino-1-carboxylic acid |
| CN104041496A (en) * | 2014-06-18 | 2014-09-17 | 北京燕化永乐生物科技股份有限公司 | Compound bactericide |
| CN104285945A (en) * | 2014-09-26 | 2015-01-21 | 郑州大学 | Phenazine-1-carboxylic acid (PCA) water suspension and preparation method thereof |
| CN104782641A (en) * | 2015-03-26 | 2015-07-22 | 山东省果树研究所 | Zhongshengmycin-containing biological sterilization composition and application thereof |
| CN107306999A (en) * | 2016-04-26 | 2017-11-03 | 上海农乐生物制品股份有限公司 | A kind of bactericidal composition based on shenqinmycin and the bactericide containing said composition |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1802927A (en) * | 2006-01-19 | 2006-07-19 | 上海交通大学 | Method for preparing bactericidal agent using antagonizing bacteria M18 strain |
| CN101973946A (en) * | 2010-10-15 | 2011-02-16 | 上海交通大学 | Phenazine-1-carboxylic acids derivative and preparation method thereof |
-
2012
- 2012-09-18 CN CN 201210348241 patent/CN102812956B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1802927A (en) * | 2006-01-19 | 2006-07-19 | 上海交通大学 | Method for preparing bactericidal agent using antagonizing bacteria M18 strain |
| CN101973946A (en) * | 2010-10-15 | 2011-02-16 | 上海交通大学 | Phenazine-1-carboxylic acids derivative and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 许煜泉: "绿色微生物源抗菌剂申嗪霉素(M18)", 《精细与专用化学品》, vol. 12, no. 20, 6 August 2005 (2005-08-06), pages 26 - 27 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103960245A (en) * | 2011-12-18 | 2014-08-06 | 深圳诺普信农化股份有限公司 | Sterilization composition containing phenazino-1-carboxylic acid |
| CN103960245B (en) * | 2011-12-18 | 2015-06-24 | 深圳诺普信农化股份有限公司 | Sterilization composition containing phenazino-1-carboxylic acid |
| CN103271094A (en) * | 2013-06-19 | 2013-09-04 | 北京燕化永乐农药有限公司 | Compound bactericide |
| CN103444721A (en) * | 2013-09-05 | 2013-12-18 | 肇庆长龙生物科技有限公司 | Sterilization pesticide composition, pesticide preparation and application of pesticide preparation |
| CN103444721B (en) * | 2013-09-05 | 2017-06-13 | 惠州长龙生物技术有限公司 | A kind of bactericidal pesticide composition, pesticidal preparations and application thereof |
| CN103651430B (en) * | 2013-12-17 | 2015-01-28 | 北京燕化永乐生物科技股份有限公司 | Compound fungicide |
| CN103651430A (en) * | 2013-12-17 | 2014-03-26 | 北京燕化永乐生物科技股份有限公司 | Compound fungicide |
| CN104041496A (en) * | 2014-06-18 | 2014-09-17 | 北京燕化永乐生物科技股份有限公司 | Compound bactericide |
| CN104041496B (en) * | 2014-06-18 | 2015-11-18 | 北京燕化永乐生物科技股份有限公司 | A kind of composite bactericide |
| CN104285945A (en) * | 2014-09-26 | 2015-01-21 | 郑州大学 | Phenazine-1-carboxylic acid (PCA) water suspension and preparation method thereof |
| CN104285945B (en) * | 2014-09-26 | 2016-07-06 | 郑州大学 | A kind of shenqinmycin aqueous suspension agent and preparation method thereof |
| CN104782641A (en) * | 2015-03-26 | 2015-07-22 | 山东省果树研究所 | Zhongshengmycin-containing biological sterilization composition and application thereof |
| CN104782641B (en) * | 2015-03-26 | 2016-05-25 | 山东省果树研究所 | Containing biological antibiotic composition and the application thereof of Zhongshengmycin |
| CN107306999A (en) * | 2016-04-26 | 2017-11-03 | 上海农乐生物制品股份有限公司 | A kind of bactericidal composition based on shenqinmycin and the bactericide containing said composition |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102812956B (en) | 2013-08-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102812956B (en) | Bactericidal composition | |
| CN110122483A (en) | Bei Laisi bacillus soluble granule and preparation method thereof | |
| CN105475303B (en) | A kind of composition pesticide containing kasugarnycin and gold alloy hydride | |
| CN105707126B (en) | A kind of bactericidal composition, preparation and its application | |
| CN101828557A (en) | Pyrimidine nucleoside antibiotic and hexaconazole compound bactericide | |
| CN103271094A (en) | Compound bactericide | |
| CN103271050A (en) | Compound bactericide | |
| CN102669100B (en) | Oxine-copper and validamycin compound pesticide composition and preparation method and application thereof | |
| CN103300023A (en) | Pesticide composition | |
| CN107306999A (en) | A kind of bactericidal composition based on shenqinmycin and the bactericide containing said composition | |
| CN103598198A (en) | Triazole and nicotine compound seed treating agent and preparation method thereof | |
| CN102783488A (en) | Azoxystrobin and validamycin A composite bactericide | |
| CN104381266A (en) | Sterilization composition | |
| CN103636623A (en) | Azoxystrobin-containing bactericidal composition | |
| CN104041496B (en) | A kind of composite bactericide | |
| CN103070196A (en) | Ternary efficient bactericide for rice in seedling stage | |
| CN102057923A (en) | Germicide composition | |
| CN103314970A (en) | Bactericidal composition | |
| CN104585197A (en) | Compound bactericide | |
| CN104430390A (en) | Amino-oligosaccharin and ethaboxam compounded agricultural fungicide | |
| CN103636619A (en) | Compound bactericide | |
| CN104255801A (en) | Crop potassium supplement composition with sterilization function as well as preparation method and application of crop potassium supplement composition | |
| CN105309434A (en) | Method for improving crop growth | |
| CN104322499B (en) | A kind of composite bactericide | |
| CN104381267B (en) | A kind of bactericidal composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: BEIJING YANHUA YONGLE BIOTECHNOLOGY CO., LTD. Free format text: FORMER NAME: BEIJING YOLOO PESTICIDE CO., LTD. |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 101105 Beijing city Tongzhou District Yongle Town East Village Naruhito service Patentee after: Beijing Yanhua Yongle Biotechnology Co., Ltd. Address before: 101105 Beijing city Tongzhou District Yongle Town East Village Naruhito service Patentee before: Beijing Yoloo Pesticide Co., Ltd. |
|
| C56 | Change in the name or address of the patentee | ||
| CP02 | Change in the address of a patent holder |
Address after: 102488, Beijing, Fangshan District Star Street, No. 1 hospital green sail, No. 16, building 8 Patentee after: Beijing Yanhua Yongle Biotechnology Co., Ltd. Address before: 101105 Beijing city Tongzhou District Yongle Town East Village Naruhito service Patentee before: Beijing Yanhua Yongle Biotechnology Co., Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20171225 Address after: 063600 Leting County Economic Development Zone, Tangshan City, Hebei Patentee after: Yanhua Yongle (Leting) Biological Technology Co. Ltd. Address before: 102488, Beijing, Fangshan District Star Street, No. 1 hospital green sail, No. 16, building 8 Patentee before: Beijing Yanhua Yongle Biotechnology Co., Ltd. |