CN103960245A - Sterilization composition containing phenazino-1-carboxylic acid - Google Patents
Sterilization composition containing phenazino-1-carboxylic acid Download PDFInfo
- Publication number
- CN103960245A CN103960245A CN201410193082.6A CN201410193082A CN103960245A CN 103960245 A CN103960245 A CN 103960245A CN 201410193082 A CN201410193082 A CN 201410193082A CN 103960245 A CN103960245 A CN 103960245A
- Authority
- CN
- China
- Prior art keywords
- shen piperazine
- piperazine mycin
- composition
- mycin
- grams
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention relates to a sterilization composition containing phenazino-1-carboxylic acid. The sterilization composition comprises an effective component of one of phenazino-1-carboxylic acid (A) and trifloxystrobin (B), wherein the mass ratio of the effective component (A) and the effective component (B) is (50:1)-(1:50). The sterilization composition provided by the invention is mainly applied to prevention and treatment on fungous diseases of cucurbits and vegetables, apples and field crops.
Description
The application is dividing an application of original application " bactericidal composition that contains Shen piperazine mycin ", and the application number of original application is 201110437597.2, and on December 18 2011 applying date, patent of invention name is called " bactericidal composition that contains Shen piperazine mycin ".
Technical field
The present invention relates to a kind of bactericidal composition, active ingredient is a kind of of Shen piperazine mycin (A) and methoxy acrylic ester compounds (B).
Background technology
Shen piperazine mycin is the tunning by pseudomonas fluorescens strain M18, main component is phenazine-1-carboxylic acid (phenazine-1-carboxylic acid), and the various plants pathogens such as rice sheath blight disease, the climing rot of cucumber, watermelon blight are had to good inhibitory action.Shen piperazine mycin in Shanghai, the ground such as Hubei, Hainan, Henan, Gansu carried out large-area extend trial, and rice sheath blight disease, alternaria leaf spot of apple, watermelon blight, gummy stem blight of melon etc. are shown to good field efficacy.
Methoxy acrylic ester compounds is a kind of extremely outstanding disinfectant use in agriculture, mainly disturbs the mitochondrial respiration of pathogen, suppresses cellular energy circulation.This series bactericidal agent mainly comprises kresoxim-methyl, ZEN 90160, Fluoxastrobin etc., has protection, treatment, eradicant action, is widely used in the important disease control of fruit tree, Cereal, vegetable category etc.It is efficient.Low toxicity, environmentally safe, with other conventional bactericide without cross resistance.But this type of compound effects site is single, easily causes the resistance of pathogen.
Under high density, high-intensity farming pattern, chemical agent is one of important measures of administering day by day serious damage by disease and insect, and guarantee agricultural production has been brought into play to important meaning.When noval chemical compound is initial, having an outstanding effect, often there is decline to a certain degree in the activity after long-term, single use.As how kresoxim-methyl has the report that resistance occurs in the world, the effect of powdery mildew is not equal to before.It is the effective means of comprehensive regulation resistance that agriculture chemical compounding is used, and noval chemical compound and the composite use of other composition are convenient to applying of noval chemical compound, is beneficial to and controls developing of disease resistance.
Given this, the combination that exploitation contains Shen piperazine mycin and methoxy acrylic ester compounds has extremely important application value.
Summary of the invention
The object of the invention is to: a kind of bactericidal composition that contains Shen piperazine mycin and methoxy acrylic ester compounds is provided.Through test a large amount of, that repeat, unexpected discovery Shen piperazine mycin and methoxy acrylic ester compounds have excellent synergy, and both are composite both can improve control efficiency, increase the control spectrum of Shen piperazine mycin, can delay mutually resistance separately again.
Technical scheme of the present invention is:
A bactericidal composition, active ingredient is by a kind of composite composition of Shen piperazine mycin (A) and methoxy acrylic ester compounds (B).
Described bactericidal composition, is characterized in that active ingredient in composition (A) and mass ratio (B) are 50: 1～1: 50, and preferably mass percent is 30: 1～1: 30, and better mass percent is 20: 1～1: 20.
Wherein said methoxy acrylic ester compounds comprises:
The molecular formula of kresoxim-methyl (English general kresoxim-methyl by name) is C
18h
19nO
4, chemical name is methyl (E)-2-methoxyimino-2-[2-(0-toloxyl) phenyl] and acetate;
The molecular formula of Fluoxastrobin (English common name azoxystrobin) is C
22h
17n
3o
5, chemical name is (E)-2-{2-[6 (2-cyano-benzene oxygen) pyrimidine-4-yl oxygen] and phenyl }-3-methoxy acrylate;
The molecular formula of oxime mattress ester (English general trifloxystrobin by name) is C
20h
19f
3n
2o
4, chemical name is methyl (E)-methoxyl group imido grpup-{ (E)-α-[1-(α, the fluoro-m-tolyl of α-tri-)-ethyleneimino oxygen base]-o-tolyl } ethyl acetate;
The molecular formula of ZEN 90160 (English general picoxystrobin by name) is C
18h
16f
3nO
4, chemical name is (E)-3-methoxy-2-[2-[6-(trifluoromethyl)-2-pyridine oxygen methyl] and phenyl] methyl acrylate;
The molecular formula of Enestroburin (English general enostroburin by name) is C
22h
27c
1nO
4, chemical name is α-[[[[4-(4-chlorphenyl)-Ding-3-alkene-2-base-] imido grpup] methyl] phenyl-'beta '-methoxy acrylic acid methyl esters;
The molecular formula of alkene oxime amine is C
21h
21c
12n
3o
4, chemical name is N-methyl-2-[((((1-methyl-3-(2,6-dichlorophenyl)-2-acrylic) and imido grpup) oxygen base) methyl) phenyl]-2-methoxyimino acetic amide;
The molecular formula of pyraclostrobin (English general pyraclostrobin by name) is C
19h
18cIN
3o
4, chemical name is methyl (N)-[[[1-(4-chlorobenzene) pyrazoles-3 base)-oxygen]-0-methoxyl group] and-N-methoxymethyl carbamate.
Described bactericidal composition, is characterized in that active ingredient accounts for 1%～90% of composition total weight.
Described bactericidal composition, is characterized in that said composition can be made into suspending agent, wetting powder, seed coat agent, aqueous emulsion, microemulsion, missible oil, granule, water dispersible granules, and can need to use corresponding solvent, carrier and auxiliary agent according to exploitation.
Suitable solvent, auxiliary agent comprise: one or more combinations of toluene, dimethylbenzene, carrene, chloroform, paraffin, kerosene, diesel oil, methyl alcohol, ethanol, propyl alcohol, butanols, amylalcohol, cyclohexanol, phenmethylol, cyclohexanone, isophorone, pyrrolidones, ethyl acetate, butyl acetate, ethylene acetate, methyl-sulfoxide etc.
Carrier comprises one or more combinations of clay, bentonite, attapulgite, calcium sulphate, precipitated calcium carbonate, sucrose, industrial chitin, silica gel, talcum powder, white carbon, sericite in powder etc.;
Available auxiliary agent comprises phosphoric acid salt, hydrochloric acid salt, lignosulphonates, naphthalenesulfonate, phenolsulfonic acid salt, succinic acid Sulfonates, polystyrene-based phenol polyethenoxy ether sulfate, Pluronic PE 6800, molecular weight is the macromolecular grafted copolymer of 20000-30000, triphenyl vinyl phenol polyoxyethylene poly-oxygen propylene aether, polystyrene-based phenol polyethenoxy ethers, polyoxyethylene nonylphenol ether class, polystyrene-based polyethenoxy ether class, alkyl naphthalene sulfonic acid salt, fatty alcohol polyethenoxy ether class, dialkyl succinylsuccinate Sulfonates, branched fatty alcohol polyethenoxy ether class, alkyl polyoxyethylene ether class, carboxyl alkyl cellulose class, hydroxy alkyl cellulose class, Magnesiumaluminumsilicate, xanthans, poly-alkyl silicon ethylene oxide, phosphoric acid, glacial acetic acid, citric acid, maleic acid, butylated hydroxy-a alkane, butylated hydroxy anisole, ditert-butylhydro quinone, one or more combinations in n-propyl gallate etc.
Described bactericidal composition is mainly used in the fungal disease of control control melon dish crop, apple crop and field crop.The main controlling object of bactericidal composition comprises: the climing rot of cucumber (Mycosphaerella melonis (Pass.) Chiu et Walker), watermelon blight (Fusarium oxysporumf.sp.Niveum, FON), Fusarium solani (Fusarium solani (Mart.) App.et woll.)), capsicum epidemic disease (Phytophthora capsici Leonian), pear scab (Venturia pirina (Cooke) Adh.), alternaria leaf spot of apple (Alternaria alternata f.sp.mali), lawn fusarium wilt (Pythium spp.P.aultimum, P.aphanidermatum), rice sheath blight disease (Rhizoctonia solani), false smut (Ustilaginoidea oryzae (Patou.) Bref=U.virens (Cooke) Tak.), rice blast (Pyricularia oryzae Cav.) etc.
Embodiment
Different compounds are carried out to applied in any combination, are one of effective control measures of administering the disease-resistant problem of damage by disease and insect.Synergistic combination can improve the control efficiency of medicament significantly, reduces the usage amount of agricultural chemicals.By a large amount of concrete composite tests, unexpected discovery Shen piperazine mycin and a kind of of methoxy acrylic ester compounds have significant synergies.The embodiment providing is below for elaborating the present invention, and do not form limiting the scope of the invention.Embodiment experimental technique used, if without specified otherwise, is conventional method; Percentage composition in embodiment, if without specified otherwise, is weight percentage.
Biologicall test example 1: the composite Toxicity Determination to rice sheath blight disease of Shen piperazine mycin and kresoxim-methyl
Subjects: rice sheath blight disease (Rhizoctonia solani), is gathered by field, through indoor separation and purification.
Test method: with reference to the agricultural industry criteria NY/T1156.5-2006 > > of the < < People's Republic of China (PRC), adopt Broad Bean Leaves method.Select susceptible broad bean potted plant, clip same area, growing way be consistent, be with petiolate blade, and moisturizing is processed, and is placed in culture dish.With the former medicine of acetone solution, the Tween 80 water dilution with 0.1%, prepares 6 series mass concentration.Blade is fully infiltrated in pre-configured liquid to 5s, drain unnecessary liquid, after natural air drying, with inoculator, have the one side of mycelia to be inoculated in diameter 5mm bacterium cake and process blade central authorities, 30 leaves of every processing inoculation, after mark, moisturizing is cultivated.Test is not established and is made blank containing the processing of medicament.Depending on blank incidence, measure the length and width (mm) of scab diameter, calculate control efficiency.
Control efficiency (%)=(blank scab diameter-chemicals treatment scab diameter) * 100/ blank scab diameter
By method of least squares, calculate concentration EC in inhibition
50, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun.Work as CTC<80, composition performance, for antagonism, is worked as 80<CTC<120, and composition performance, for summation action, is worked as CTC>120, and composition performance is synergistic effect.
Actual measurement toxicity index (ATI)=(standard medicament EC
50/ reagent agent EC
50) * 100
The percentage composition of B in percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture
Co-toxicity coefficient (CTC)=[the theoretical toxicity index (TTI) of mixture actual measurement toxicity index (ATI)/mixture] * 100
Toxicity test the results are shown in Table 1.
The composite Toxicity Determination result to rice sheath blight disease of table 1 Shen piperazine mycin and kresoxim-methyl
| Process | EC 50(μg/mL) | ATI | TTI | Co-toxicity coefficient CTC |
| Shen piperazine mycin | 1.25 | 100.0 | / | / |
| Kresoxim-methyl | 0.69 | 181.2 | / | / |
| Shen piperazine mycin 50: kresoxim-methyl 1 | 0.98 | 127.6 | 101.6 | 125.6 |
| Shen piperazine mycin 30: kresoxim-methyl 1 | 0.73 | 171.2 | 102.6 | 166.9 |
| Shen piperazine mycin 20: kresoxim-methyl 1 | 0.6 | 208.3 | 103.9 | 200.6 |
| Shen piperazine mycin 10: kresoxim-methyl 1 | 0.33 | 378.8 | 107.4 | 352.8 |
| Shen piperazine mycin 1: kresoxim-methyl 1 | 0.27 | 463.0 | 140.6 | 329.3 |
| Shen piperazine mycin 1: kresoxim-methyl 10 | 0.31 | 403.2 | 173.8 | 232.0 |
| Shen piperazine mycin 1: kresoxim-methyl 20 | 0.37 | 337.8 | 177.3 | 190.6 |
| Shen piperazine mycin 1: kresoxim-methyl 30 | 0.43 | 290.7 | 178.5 | 162.8 |
| Shen piperazine mycin 1: kresoxim-methyl 50 | 0.57 | 219.3 | 179.6 | 122.1 |
Biologicall test example 2: Shen piperazine mycin and the Toxicity Determination of azoxystrobin compound to watermelon blight
Subjects: watermelon blight (Fusarium oxysporum f.sp.Niveum, FON), is gathered by field, through indoor separation and purification.
Test method: with reference to the agricultural industry criteria NY/T1156.2-2006 > > of the < < People's Republic of China (PRC), mycelial growth rate method.
Test method and statistical method, with biologicall test example 1, are calculated concentration EC in inhibition by method of least squares
50, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun.Work as CTC<80, composition performance, for antagonism, is worked as 80<CTC<120, and composition performance, for summation action, is worked as CTC>120, and composition performance is synergistic effect.
Toxicity test the results are shown in Table 2.
Table 2 Shen piperazine mycin and the azoxystrobin compound Toxicity Determination result to watermelon blight
| Process | EC 50(μg/mL) | ATI | TTI | Co-toxicity coefficient CTC |
| Shen piperazine mycin | 1.168 | 100.0 | / | / |
| Fluoxastrobin | 0.61 | 191.5 | / | / |
| Shen piperazine mycin 50: Fluoxastrobin 1 | 0.885 | 132.0 | 101.8 | 129.7 |
| Shen piperazine mycin 30: Fluoxastrobin 1 | 0.701 | 166.6 | 103.0 | 161.8 |
| Shen piperazine mycin 20: Fluoxastrobin 1 | 0.327 | 357.2 | 104.4 | 342.3 |
| Shen piperazine mycin 10: Fluoxastrobin 1 | 0.211 | 553.6 | 108.3 | 511.1 |
| Shen piperazine mycin 1: Fluoxastrobin 1 | 0.099 | 1179.8 | 145.7 | 809.5 |
| Shen piperazine mycin 1: Fluoxastrobin 10 | 0.145 | 805.5 | 183.2 | 439.8 |
| Shen piperazine mycin 1: Fluoxastrobin 20 | 0.274 | 426.3 | 187.1 | 227.8 |
| Shen piperazine mycin 1: Fluoxastrobin 30 | 0.382 | 305.8 | 188.5 | 162.2 |
| Shen piperazine mycin 1: Fluoxastrobin 50 | 0.501 | 233.1 | 189.7 | 122.9 |
Biologicall test example 3: the composite Toxicity Determination to the climing rot of cucumber of Shen piperazine mycin and oxime bacterium ester
Subjects: the climing rot of cucumber (Mycosphaerella melonis (Pass.) Chiu et Walker), is gathered by field, through indoor separation and purification.
Test method: with reference to the agricultural industry criteria NY/T1156.2-2006 > > of the < < People's Republic of China (PRC), mycelial growth rate method.With the former medicine of acetone solution, 0.1% Tween 80 water dilution, is prepared into 6 series mass concentration.Under aseptic condition, get the liquid 5mL configuring and mix with 75mL medium, make 4 pastille flat boards, with equivalent sterile water, mix in contrast with medium.Cultured cucumber didymella bryoniae sterilizing card punch with diameter 5mm under aseptic condition is cut to bacterium cake from edge.With inoculator, pure culture biscuits involvng inoculation is dull and stereotyped central in pastille, mycelia faces down, and is placed in 25 ℃ of incubators.Depending on blank growing state investigation result, each bacterium colony with right-angled intersection method vertical survey diameter respectively once, is got its mean value, calculates mycelial growth inhibition rate.Mycelial growth inhibition rate=(blank bacterium colony increases diameter-chemicals treatment bacterium colony and increases diameter) * 100/ blank bacterium colony increases diameter.
By method of least squares, calculate concentration EC in inhibition
50, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun.Statistical method is with biologicall test example 1.Work as CTC<80, composition performance, for antagonism, is worked as 80<CTC<120, and composition performance, for summation action, is worked as CTC>120, and composition performance is synergistic effect.
Toxicity test the results are shown in Table 3.
The composite Toxicity Determination result to the climing rot of cucumber of table 3 Shen piperazine mycin and oxime bacterium ester
| Process | EC 50(μg/mL) | ATI | TTI | Co-toxicity coefficient CTC |
| Shen piperazine mycin | 0.646 | 100.0 | / | / |
| Oxime bacterium ester | 0.228 | 283.3 | / | / |
| Shen piperazine mycin 50: oxime bacterium ester 1 | 0.494 | 130.8 | 103.6 | 126.2 |
| Shen piperazine mycin 30: oxime bacterium ester 1 | 0.365 | 177.0 | 105.9 | 167.1 |
| Shen piperazine mycin 20: oxime bacterium ester 1 | 0.228 | 283.3 | 108.7 | 260.6 |
| Shen piperazine mycin 10: oxime bacterium ester 1 | 0.098 | 659.2 | 116.7 | 565.0 |
| Shen piperazine mycin 1: oxime bacterium ester 1 | 0.038 | 1700.0 | 191.7 | 887.0 |
| Shen piperazine mycin 1: oxime bacterium ester 10 | 0.046 | 1404.3 | 266.7 | 526.6 |
| Shen piperazine mycin 1: oxime bacterium ester 20 | 0.076 | 850.0 | 274.6 | 309.5 |
| Shen piperazine mycin 1: oxime bacterium ester 30 | 0.145 | 445.5 | 277.4 | 160.6 |
| Shen piperazine mycin 1: oxime bacterium ester 50 | 0.189 | 341.8 | 279.7 | 122.2 |
Biologicall test example 4: the composite Toxicity Determination to the climing rot of cucumber of Shen piperazine mycin and ZEN 90160
Subjects: the climing rot of cucumber (Mycosphaerella melonis (Pass.) Chiu et Walker), is gathered by field, through indoor separation and purification.
Test method: with reference to the agricultural industry criteria NY/T1156.2-2006 > > of the < < People's Republic of China (PRC), mycelial growth rate method.Test method, statistical method are with biologicall test example 3.By method of least squares, calculate concentration EC in inhibition
50, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun.Work as CTC<80, composition performance, for antagonism, is worked as 80<CTC<120, and composition performance, for summation action, is worked as CTC>120, and composition performance is synergistic effect.
Toxicity test the results are shown in Table 4.
The composite Toxicity Determination result to the climing rot of cucumber of table 4 Shen piperazine mycin and ZEN 90160
| Process | EC 50(μg/mL) | ATI | TTI | Co-toxicity coefficient CTC |
| Shen piperazine mycin | 0.637 | 100.0 | / | / |
| ZEN 90160 | 0.177 | 359.9 | / | / |
| Shen piperazine mycin 50: ZEN 90160 1 | 0.496 | 128.4 | 105.1 | 122.2 |
| Shen piperazine mycin 30: ZEN 90160 1 | 0.283 | 225.1 | 108.4 | 207.7 |
| Shen piperazine mycin 20: ZEN 90160 1 | 0.177 | 359.9 | 112.4 | 320.3 |
| Shen piperazine mycin 10: ZEN 90160 1 | 0.029 | 2196.6 | 123.6 | 1776.8 |
| Shen piperazine mycin 1: ZEN 90160 1 | 0.035 | 1820.0 | 229.9 | 791.5 |
| Shen piperazine mycin 1: ZEN 90160 10 | 0.051 | 1249.0 | 336.3 | 371.4 |
| Shen piperazine mycin 1: ZEN 90160 20 | 0.066 | 965.2 | 347.5 | 277.7 |
| Shen piperazine mycin 1: ZEN 90160 30 | 0.106 | 600.9 | 351.5 | 171.0 |
| Shen piperazine mycin 1: ZEN 90160 50 | 0.142 | 448.6 | 354.8 | 126.4 |
Biologicall test example 5: the composite Toxicity Determination to rice sheath blight disease of Shen piperazine mycin and Enestroburin
Subjects: rice sheath blight disease (Rhizoctonia solani), is gathered by field, through indoor separation and purification.
Test method: with reference to the agricultural industry criteria NY/T1156.5-2006 > > of the < < People's Republic of China (PRC), adopt Broad Bean Leaves method, test method and statistical method are with biologicall test example 1.
By method of least squares, calculate concentration EC in inhibition
50, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun.Work as CTC<80, composition performance, for antagonism, is worked as 80<CTC<120, and composition performance, for summation action, is worked as CTC>120, and composition performance is synergistic effect.
Actual measurement toxicity index (ATI)=(standard medicament EC
50/ reagent agent EC
50) * 100
The percentage composition of B in percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture
Co-toxicity coefficient (CTC)=[the theoretical toxicity index (TTI) of mixture actual measurement toxicity index (ATI)/mixture] * 100
Toxicity test the results are shown in Table 5.
The composite Toxicity Determination result to rice sheath blight disease of table 5 Shen piperazine mycin and Enestroburin
| Process | EC 50(μg/mL) | ATI | TTI | Co-toxicity coefficient CTC |
| Shen piperazine mycin | 1.18 | 100.0 | / | / |
| Enestroburin | 0.76 | 155.3 | / | / |
| Shen piperazine mycin 50: Enestroburin 1 | 0.93 | 126.9 | 101.1 | 125.5 |
| Shen piperazine mycin 30: Enestroburin 1 | 0.68 | 173.5 | 101.8 | 170.5 |
| Shen piperazine mycin 20: Enestroburin 1 | 0.54 | 218.5 | 102.6 | 212.9 |
| Shen piperazine mycin 10: Enestroburin 1 | 0.38 | 310.5 | 105.0 | 295.7 |
| Shen piperazine mycin 1: Enestroburin 1 | 0.21 | 561.9 | 127.6 | 440.3 |
| Shen piperazine mycin 1: Enestroburin 10 | 0.33 | 357.6 | 150.2 | 238.0 |
| Shen piperazine mycin 1: Enestroburin 20 | 0.4 | 295.0 | 152.6 | 193.3 |
| Shen piperazine mycin 1: Enestroburin 30 | 0.47 | 251.1 | 153.5 | 163.6 |
| Shen piperazine mycin 1: Enestroburin 50 | 0.62 | 190.3 | 154.2 | 123.4 |
Biologicall test example 6: the composite Toxicity Determination to rice sheath blight disease of Shen piperazine mycin and alkene oxime amine
Subjects: rice sheath blight disease (Rhizoctonia solani), is gathered by field, through indoor separation and purification.
Test method: with reference to the agricultural industry criteria NY/T1156.5-2006 > > of the < < People's Republic of China (PRC), adopt Broad Bean Leaves method, test method and statistical method are with biologicall test example 1.
Toxicity test the results are shown in Table 6.
The composite Toxicity Determination result to rice sheath blight disease of table 6 Shen piperazine mycin and alkene oxime amine
| Process | EC 50(μg/mL) | ATI | TTI | Co-toxicity coefficient CTC |
| Shen piperazine mycin | 1.22 | 100.0 | / | / |
| Alkene oxime amine | 0.83 | 147.0 | / | / |
| Shen piperazine mycin 50: alkene oxime amine 1 | 0.96 | 127.1 | 100.9 | 125.9 |
| Shen piperazine mycin 30: alkene oxime amine 1 | 0.71 | 171.8 | 101.5 | 169.3 |
| Shen piperazine mycin 20: alkene oxime amine 1 | 0.56 | 217.9 | 102.2 | 213.1 |
| Shen piperazine mycin 10: alkene oxime amine 1 | 0.31 | 393.5 | 104.3 | 377.4 |
| Shen piperazine mycin 1: alkene oxime amine 1 | 0.21 | 581.0 | 123.5 | 470.4 |
| Shen piperazine mycin 1: alkene oxime amine 10 | 0.32 | 381.3 | 142.7 | 267.1 |
| Shen piperazine mycin 1: alkene oxime amine 20 | 0.41 | 297.6 | 144.8 | 205.6 |
| Shen piperazine mycin 1: alkene oxime amine 30 | 0.52 | 234.6 | 145.5 | 161.3 |
| Shen piperazine mycin 1: alkene oxime amine 50 | 0.69 | 176.8 | 146.1 | 121.0 |
Biologicall test example 7: the composite Toxicity Determination to watermelon blight of Shen piperazine mycin and pyraclostrobin
Subjects: watermelon blight (Fusarium oxysporum f.sp.Niveum, FON), is gathered by field, through indoor separation and purification.
Test method: with reference to the agricultural industry criteria NY/T1156.2-2006 > > of the < < People's Republic of China (PRC), mycelial growth rate method.
Test method and statistical method, with biologicall test example 4, are calculated concentration EC in inhibition by method of least squares
50, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun.Work as CTC<80, composition performance, for antagonism, is worked as 80<CTC<120, and composition performance, for summation action, is worked as CTC>120, and composition performance is synergistic effect.
Toxicity test the results are shown in Table 7.
The composite Toxicity Determination result to watermelon blight of table 7 Shen piperazine mycin and pyraclostrobin
Table 1 to table 7 points out, Shen piperazine mycin and methoxy acrylic ester compounds, proportioning 50: 1～1: 50, have good synergistic effect, especially, between 30: 1～1: 30, embody more significant synergistic effect, and co-toxicity coefficient is all more than 160.
Bactericidal composition of the present invention can be prepared into by known method suspending agent, wetting powder, seed coat agent, aqueous emulsion, microemulsion, missible oil, granule, water dispersible granules.With specific embodiment, describe below, in formula, percentage is mass percent.Active component Shen piperazine mycin and methoxy acrylic ester compounds compounded combination in varing proportions in present specification, methoxy acrylic ester compounds in single embodiment all can be substituted or replace by other methoxy acrylic ester compounds, forms new embodiment.
Embodiment 1:51% Shen piperazine mycin kresoxim-methyl suspending agent
Shen piperazine mycin 50%, kresoxim-methyl 1%, methyl naphthalene sulfonate formaldehyde condensate 8%, sodium lignosulfonate 15%, xanthans 3%, silicone oil 5%, glycerine 8%, all the other water complement to 100%.Active component, dispersant and thickener are added in sand mill and processed successively in the ratio of formula, then send in mixer for well-distribution, even through shear agitation, make 51% Shen piperazine mycin kresoxim-methyl suspending agent.
This embodiment is applied to prevent and treat rice sheath blight disease.By 51% Shen piperazine mycin kresoxim-methyl suspending agent, by 200 grams/ha of active ingredients, (effective dose of Shen piperazine mycin is about 196 grams/ha, the effective dose of kresoxim-methyl is about 4 grams/ha) thin up spraying, every dispenser in 7 days once, dispenser is three times continuously, and control efficiency is respectively 96.1%.1% Shen piperazine mycin suspending agent, 30% kresoxim-methyl suspending agent are processed in the same way by 300 grams/ha, 200 grams/ha of active ingredients respectively, and the preventive effect after medicine is respectively 83.7%, 82.6%.The composite rear synergistic effect of Shen piperazine mycin and kresoxim-methyl is obvious, and the consumption of the active ingredient in composition is lower than the consumption of single dose, the preventive effect to the preventive effect of rice sheath blight disease significantly better than single dose.
Embodiment 2:62% Shen piperazine mycin Enestroburin wetting powder
Shen piperazine mycin 60%, Enestroburin 2%, pulls open powder 0.5%, lauryl sodium sulfate 2%, white carbon 5%, attapulgite complements to 100%.Each components such as active component, dispersant, wetting agent are mixed in the ratio of formula, after air-flow shatters, obtain 62% Shen piperazine mycin Enestroburin wetting powder.
This embodiment is applied to prevent and treat capsicum epidemic disease.By 62% Shen piperazine mycin Enestroburin wetting powder, by 250 grams/ha of active ingredients, (effective dose of Shen piperazine mycin is about 242 grams/ha, the effective dose of Enestroburin is about 8 grams/ha) thin up spraying, every dispenser in 7 days once, dispenser is three times continuously, and the control efficiency after medicine is 98.6%.1% Shen piperazine mycin suspending agent, 40% Enestroburin suspending agent are in kind used by 300 grams/ha of active ingredients, 200 grams/ha respectively, and the preventive effect after medicine is respectively 74.1%, 81.2%.The composite rear synergistic effect of Shen piperazine mycin and Enestroburin is obvious, and the effective ingredient consumption in composition is lower than the consumption of single dose, the preventive effect to the preventive effect of capsicum epidemic disease significantly better than single dose.
Embodiment 3:42% Shen piperazine mycin alkene oxime amine seed coat agent
Shen piperazine mycin 40%, alkene oxime amine 2%, methyl naphthalene sulfonate formaldehyde condensate 8%, sodium lignosulfonate 10%, xanthans 3%, sodium carboxymethylcellulose 3%, glycerine 8%, polyvinylpyrrolidone 2%, acid scarlet 0.5%, all the other water complement to 100%.Active component, dispersant and thickener are added in sand mill and processed successively in the ratio of formula, then send in mixer for well-distribution, even through shear agitation, regulate pH value, make 42% Shen piperazine mycin alkene oxime amine seed coat agent.
This embodiment is applied to prevent and treat Fusarium solani.The ratio seed dressing by 3 grams of active ingredients in every double centner seed by 42% Shen piperazine mycin alkene oxime amine seed coat agent, the control efficiency after medicine is 97.5%.1% Shen piperazine mycin seed coat agent, the respectively every double centner seed of 40% alkene oxime amine seed coat agent are dressed seed by the ratio of 4 grams, 2 grams of active ingredients, and the preventive effect after medicine is respectively 76.2%, 71.8%.The composite rear synergistic effect of Shen piperazine mycin and alkene oxime amine is obvious, and the consumption of the active ingredient in composition is lower than the consumption of single dose, the preventive effect to the preventive effect of Fusarium solani significantly better than single dose.
Embodiment 4:11% Shen piperazine mycin trifloxystrobin water emulsion
Shen piperazine mycin 10%, oxime bacterium ester 1%, 1-METHYLPYRROLIDONE 10%, calcium dodecyl benzene sulfonate 5%, agriculture breast 600#5%, water complements to 100%.Former medicine, solvent, emulsifier are added together, make to be dissolved into even oil phase; Water-soluble component and water mixing are made to water; Under high-speed stirred, oil phase is mixed with water, make 11% Shen piperazine mycin trifloxystrobin water emulsion.
This embodiment is applied to prevent and treat alternaria leaf spot of apple.By 11% Shen piperazine mycin trifloxystrobin water emulsion, by 200 grams/ha of active ingredients, (effective dose of Shen piperazine mycin is about 182 grams/ha, the effective dose of oxime bacterium ester is about 18 grams/ha) thin up spraying, every dispenser in 7 days once, dispenser is three times continuously, and the control efficiency after medicine is 98.2%.1% Shen piperazine mycin suspending agent, 50% oxime bacterium ester suspending agent are in kind used by 300 grams/ha, 300 grams/ha respectively, and the preventive effect after medicine is respectively 86.7%, 81.1%.The composite rear synergistic effect of Shen piperazine mycin and oxime bacterium ester is obvious, and the consumption of the active ingredient in composition is lower than the consumption of single dose, the preventive effect to the preventive effect of alternaria leaf spot of apple significantly better than single dose.
Embodiment 5:2% Shen piperazine mycin ZEN 90160 microemulsion
Shen piperazine mycin 1%, ZEN 90160 1%, 1-METHYLPYRROLIDONE 10%, isopropyl alcohol 10%, agriculture breast 500#5%, agriculture breast 1601#5%, water complements to 100%.Former medicine, solvent, emulsifier are added together, make to be dissolved into even oil phase; Water-soluble component and water mixing are made to water; Under high-speed stirred, oil phase is mixed with water, make 2% Shen piperazine mycin ZEN 90160 microemulsion.
This embodiment is applied to prevent and treat potato black scurf.By 2% Shen piperazine mycin ZEN 90160 microemulsion, by 300 grams/ha of active ingredients, (effective dose of Shen piperazine mycin is 150 grams/ha, the effective dose of ZEN 90160 is 150 grams/ha) thin up spraying, every dispenser in 7 days once, dispenser is three times continuously, and the control efficiency after medicine is 97.5%.1% Shen piperazine mycin suspending agent, 50% ZEN 90160 suspending agent are in kind used by 300 grams/ha, 250 grams/ha respectively, and the preventive effect after medicine is respectively 76.3%, 80.1%.The composite rear synergistic effect of Shen piperazine mycin and ZEN 90160 is obvious, and the consumption of the active ingredient in composition is lower than the consumption of single dose, the preventive effect to the preventive effect of potato black scurf significantly better than single dose.
Embodiment 6:22% Shen piperazine mycin Fluoxastrobin missible oil
Shen piperazine mycin 2%, Fluoxastrobin 20%, 1-METHYLPYRROLIDONE 8%, agriculture breast 1601#5%, agriculture breast 500#5%, dimethylbenzene complements to 100%.Active component, emulsifier and auxiliary agent are added in mixing kettle successively in the ratio of formula, stir, make 22% Shen piperazine mycin Fluoxastrobin missible oil.
This embodiment is applied to prevent and treat the climing rot of cucumber.By 22% Shen piperazine mycin Fluoxastrobin missible oil, by 200 grams/ha of active ingredients, (effective dose of Shen piperazine mycin is about 18 grams/ha, the effective dose of Fluoxastrobin is about 182 grams/ha) thin up spraying, every dispenser in 7 days once, dispenser is three times continuously, and the control efficiency after medicine is 98.2%.1% Shen piperazine mycin suspending agent, 25% Fluoxastrobin suspending agent are in kind used by 300 grams/ha, 200 grams/ha of active ingredients respectively, and the preventive effect after medicine is respectively 75.1%, 83.6%.After Shen piperazine mycin and azoxystrobin compound, synergistic effect is obvious, and the consumption of the active ingredient in composition is lower than the consumption of single dose, the preventive effect to the preventive effect of the climing rot of cucumber significantly better than single dose.
Embodiment 7:2.1% Shen piperazine mycin pyraclostrobin aqueous emulsion
Shen piperazine mycin 0.1%, pyraclostrobin 2%, 1-METHYLPYRROLIDONE 3%, calcium dodecyl benzene sulfonate 5%, agriculture breast 600#3%, water complements to 100%.Former medicine, solvent, emulsifier are added together, make to be dissolved into even oil phase; Water-soluble component and water mixing are made to water; Under high-speed stirred, oil phase is mixed with water, make 2.1% Shen piperazine mycin pyraclostrobin aqueous emulsion.
This embodiment is applied to lawn fusarium wilt.By 2.1% Shen piperazine mycin pyraclostrobin aqueous emulsion, by 150 grams/ha of active ingredients, (effective dose of Shen piperazine mycin is about 7 grams/ha, the effective dose of pyraclostrobin is about 143 grams/ha) thin up spraying, every dispenser in 7 days once, dispenser is three times continuously, and the control efficiency after medicine is 98.4%.1% Shen piperazine mycin suspending agent, 30% pyraclostrobin suspending agent are in kind used by 300 grams/ha of active ingredients, 200 grams/ha respectively, and the preventive effect after medicine is respectively 80.1%, 86.9%.The composite rear synergistic effect of Shen piperazine mycin and pyraclostrobin is obvious, and the consumption of the active ingredient in composition is lower than the consumption of single dose, the preventive effect to the preventive effect of lawn fusarium wilt significantly better than single dose.
Embodiment 8:3.1% Shen piperazine mycin Fluoxastrobin granule
Shen piperazine mycin 0.1%, Fluoxastrobin 3%, starch 3%, xylem sodium sulfonate 3%, ammonium lauryl sulfate 0.5%, supplies 100% with slag.Active component, auxiliary agent are mixed in the ratio of formula, and mist projection granulating, with slag absorption, is drying to obtain 3.1% Shen piperazine mycin Fluoxastrobin granule.
This embodiment is applied to prevent and treat the watermelon blight in seedling stage.By 3.1% Shen piperazine mycin Fluoxastrobin granule, by 150 grams/ha of active ingredients, (effective dose of Shen piperazine mycin is about 5 grams/ha, the effective dose of Fluoxastrobin is about 145 grams/ha) spread fertilizer over the fields in plant root, every dispenser in 7 days once, dispenser is three times continuously, and control efficiency is respectively 98.6%.5% Shen piperazine mycin granule, 5% Fluoxastrobin granule are in kind processed by 300 grams/ha, 250 grams/ha respectively, and the preventive effect after medicine is respectively 76.2%, 83.5%.After Shen piperazine mycin and azoxystrobin compound, synergistic effect is obvious, and the consumption of the active ingredient in composition is lower than the consumption of single dose, the preventive effect to the preventive effect of watermelon blight significantly better than single dose.
Embodiment 9:51% Shen piperazine mycin water dispersion granules agent of Kresoxim
Shen piperazine mycin 1%, kresoxim-methyl 50%, sodium lignin sulfonate 6%, dodecyl sodium sulfate 1%, Magnesiumaluminumsilicate 3%, white carbon 3%, sodium alginate 1%, bentonite supplies 100%.Each components such as active component, dispersant, wetting agent are mixed in the ratio of formula, through air-flow crushing, add 10～30% water, then through kneading, granulation, the dry 51% Shen piperazine mycin water dispersion granules agent of Kresoxim that makes.
This embodiment is applied to prevent and treat wheat sharp eyespot.By 51% Shen piperazine mycin water dispersion granules agent of Kresoxim, (effective dose of Shen piperazine mycin is about 3 grams/ha by 150 grams/ha, the effective dose of kresoxim-methyl is about 147 grams/ha) thin up spraying, every dispenser in 7 days once, dispenser is three times continuously, and the control efficiency after medicine is 93.8%.1% Shen piperazine mycin suspending agent, 30% kresoxim-methyl suspending agent are in kind used by 300 grams/ha, 250 grams/ha respectively, and the preventive effect after medicine is respectively 81.7%, 81.3%.The composite rear synergistic effect of Shen piperazine mycin and kresoxim-methyl is obvious, and the consumption of the active ingredient in composition is lower than the consumption of single dose, the preventive effect to the preventive effect of wheat sharp eyespot significantly better than single dose.
Claims (5)
1. a bactericidal composition, active ingredient is a kind of of Shen piperazine mycin (A) and methoxy acrylic ester compounds (B), wherein active ingredient (A) is 50: 1～1: 50 with mass ratio (B), and described methoxy acrylic ester compounds is oxime bacterium ester.
2. bactericidal composition according to claim 1, is characterized in that active ingredient in composition (A) and mass ratio (B) are 30: 1～1: 30.
3. bactericidal composition according to claim 1, is characterized in that active ingredient in composition (A) and mass ratio (B) are 20: 1～1: 20.
4. according to the bactericidal composition described in claims 1 to 3 any one, it is characterized in that active ingredient accounts for 1%～90% of composition total weight.
5. bactericidal composition according to claim 4, is characterized in that said composition can be made into suspending agent, wetting powder, seed coat agent, aqueous emulsion, microemulsion, missible oil, granule, water dispersible granules.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410193082.6A CN103960245B (en) | 2011-12-18 | 2011-12-18 | Sterilization composition containing phenazino-1-carboxylic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410193082.6A CN103960245B (en) | 2011-12-18 | 2011-12-18 | Sterilization composition containing phenazino-1-carboxylic acid |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110437597.2A Division CN103155925B (en) | 2011-12-18 | 2011-12-18 | Sterilization composite containing phenazino-1-carboxylic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103960245A true CN103960245A (en) | 2014-08-06 |
| CN103960245B CN103960245B (en) | 2015-06-24 |
Family
ID=51230658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410193082.6A Active CN103960245B (en) | 2011-12-18 | 2011-12-18 | Sterilization composition containing phenazino-1-carboxylic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103960245B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106234408A (en) * | 2015-01-08 | 2016-12-21 | 四川利尔作物科学有限公司 | Bactericidal composition and application thereof |
| CN107517979A (en) * | 2016-11-22 | 2017-12-29 | 上海农乐生物制品股份有限公司 | A kind of microbicide compositions containing shenqinmycin and 2-cyano-3-amino-3-phenylancryic acetate |
| CN109090128A (en) * | 2018-09-29 | 2018-12-28 | 武汉友芹种苗技术有限公司 | A kind of bactericidal composition for preventing and treating downy mildew of lettuce |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001080640A1 (en) * | 2000-04-26 | 2001-11-01 | Bayer Aktiengesellschaft | Fungicidal mixture |
| CN1335748A (en) * | 1998-12-22 | 2002-02-13 | 阿方蒂农科股份有限公司 | Synergic fungicide composition comprising a compound analogue of strobilurin |
| CN102812956A (en) * | 2012-09-18 | 2012-12-12 | 北京燕化永乐农药有限公司 | Bactericidal composition |
-
2011
- 2011-12-18 CN CN201410193082.6A patent/CN103960245B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1335748A (en) * | 1998-12-22 | 2002-02-13 | 阿方蒂农科股份有限公司 | Synergic fungicide composition comprising a compound analogue of strobilurin |
| WO2001080640A1 (en) * | 2000-04-26 | 2001-11-01 | Bayer Aktiengesellschaft | Fungicidal mixture |
| CN102812956A (en) * | 2012-09-18 | 2012-12-12 | 北京燕化永乐农药有限公司 | Bactericidal composition |
Non-Patent Citations (1)
| Title |
|---|
| 许煜泉: "绿色微生物源抗菌剂申嗪霉素(M18)", 《精细与专用化学品》, vol. 12, no. 20, 21 October 2004 (2004-10-21) * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106234408A (en) * | 2015-01-08 | 2016-12-21 | 四川利尔作物科学有限公司 | Bactericidal composition and application thereof |
| CN107517979A (en) * | 2016-11-22 | 2017-12-29 | 上海农乐生物制品股份有限公司 | A kind of microbicide compositions containing shenqinmycin and 2-cyano-3-amino-3-phenylancryic acetate |
| CN109090128A (en) * | 2018-09-29 | 2018-12-28 | 武汉友芹种苗技术有限公司 | A kind of bactericidal composition for preventing and treating downy mildew of lettuce |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103960245B (en) | 2015-06-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103155925B (en) | Sterilization composite containing phenazino-1-carboxylic acid | |
| CN103155936B (en) | Plant disease control composition containing phenazino-1-carboxylic acid | |
| CN103155926B (en) | Sterilization composite based on phenazino-1-carboxylicacid | |
| CN101595882B (en) | Pesticide composition containing methoxyl acrylic ester bactericide | |
| CN105432620B (en) | Bactericidal composition and its application | |
| CN105961399B (en) | Bactericidal composition and its application | |
| CN101647454A (en) | Sterilizing composition containing cyprodinil | |
| CN109662096A (en) | A kind of composition pesticide containing amino-oligosaccharide and fluxapyroxad | |
| CN101653139A (en) | Compound bactericidal composition containing boscalid | |
| CN103947663A (en) | Bactericidal composition containing phenazino-1-carboxylic acid | |
| CN103120166B (en) | Hymexazol-containing sterilizing composition | |
| CN103960245B (en) | Sterilization composition containing phenazino-1-carboxylic acid | |
| CN101669524B (en) | Bactericide composition with tetraconazole | |
| CN104488896B (en) | Containing methylsulfonyl bacterium azoles and the complex composition of methoxy acrylic bactericide and preparation | |
| CN101755761B (en) | Fluquinconazole-based synergistic pesticide composition | |
| CN104106575B (en) | A kind of bactericidal composition containing pungent bacterium amine and olefin conversion | |
| CN103444721A (en) | Sterilization pesticide composition, pesticide preparation and application of pesticide preparation | |
| CN103210936B (en) | Bactericide and purpose thereof | |
| CN104430390A (en) | Amino-oligosaccharin and ethaboxam compounded agricultural fungicide | |
| CN113317319B (en) | Composite bactericide and application thereof | |
| CN107980777A (en) | A kind of bactericidal composition containing bromothalonil and eugenol | |
| CN101999379B (en) | Dithianon and mancozeb containing bactericidal composition and application thereof | |
| CN102057907B (en) | Sterilization composite containing dithianon and chlorothalonil and application thereof | |
| CN104488904B (en) | A kind of complex composition of the azoles of bacterium containing methylsulfonyl and benzimidazole germicide | |
| CN108849931A (en) | A kind of bactericidal composition and its application containing probenazole and mandipropamid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |