CN105432620B - Bactericidal composition and its application - Google Patents

Bactericidal composition and its application Download PDF

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Publication number
CN105432620B
CN105432620B CN201510771957.0A CN201510771957A CN105432620B CN 105432620 B CN105432620 B CN 105432620B CN 201510771957 A CN201510771957 A CN 201510771957A CN 105432620 B CN105432620 B CN 105432620B
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shenqinmycin
agent
bactericidal composition
bactericidal
succinate dehydrogenase
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CN105432620A (en
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古越
李翔
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Sichuan Lier Crop Science Co Ltd
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Sichuan Lier Crop Science Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/601,4-Diazines; Hydrogenated 1,4-diazines
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/18Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
    • A01N37/22Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/18Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
    • A01N37/22Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides
    • A01N37/24Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides containing at least one oxygen or sulfur atom being directly attached to the same aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of bactericidal composition and its application, it is related to technical field of pesticide combination.The active ingredient of bactericidal composition of the present invention contains shenqinmycin A and succinate dehydrogenase inhibitors series bactericidal agent B, wherein succinate dehydrogenase inhibitors series bactericidal agent B is the one or more of fluopyram, bicyclic fluorine azoles bacterium amine or fluorine azoles bacterium aniline etc., and shenqinmycin A and succinate dehydrogenase inhibitors series bactericidal agent B weight ratio are 1:100~100:1.Effective constituents A has obvious synergistic effect with B compoundings, can effectively reduce the amount of application of each single dose.To expanding fungicidal spectrum and delaying the plant resistance to the action of a drug to have preferable effect, moreover it is possible to extend the lasting period, there is obvious promotional value.Said composition can add the auxiliary element for allowing to use in other agricultural chemicals and be processed into any formulation.

Description

Bactericidal composition and its application
Technical field
The present invention relates to a kind of bactericidal composition and its application, particularly relates to a kind of active ingredient and contains shenqinmycin and amber The bactericidal composition of amber dehydrogenase inhibitor series bactericidal agent and its application, belong to technical field of compounded pesticide.
Background technology
In agricultural production, crops can healthy growth, in addition to being influenceed by worm, crop smothering, the preventing and treating to disease is also very heavy Will.Reinforcement and sustainable development implementation with environmental protection ideas, efficient, low toxicity, high activity, low-residual have turned into agricultural chemicals and sent out The inexorable trend of exhibition.However, even efficient germicide, long-term to be used alone, also easily disease is developed immunity to drugs, cause to use The problems such as dose increases, preventive effect reduces, the lasting period shortens, is unfavorable for environment sustainable development.Therefore, how Scientific Usage of Drugs, drop The dosage of low chemical pesticide, drug effect is improved, turn into the problem that plant protection art is badly in need of solving.
Shenqinmycin, for its structural formula such as shown in (I), shenqinmycin is to be secreted by pseudomonas fluorescens M18 through biological culture A kind of antibiotic, while there is wide spectrum to suppress phytopathogen and promote the bactericide of the dual-use function of Effects on Plant Growth, With wide spectrum, it is efficient the characteristics of.
SDHI classes (succinate dehydrogenase inhibitors) bactericide is to be sterilized agent resistance action committee Member's meeting (Fungicide Resistance Action Committee, FRAC) is new to be marked off a kind of mechanism of action come and resists The property similar compound of mechanism.The mechanism of action of this series bactericidal agent mainly suppresses pathogen succinate dehydrogenase activity, so as to Its respiration is disturbed, it acts on special, strong drug action, persistent, obvious effect of increasing production.This kind of of exploitation that succeeded at present kills Microbial inoculum at least 15 kinds, the major product released in recent years have biphenyl pyrrole bacterium amine (bixafen), fluopyram (fluopyram), fluorine azoles bacterium aniline (penflufen), bicyclic fluorine azoles bacterium amine (isopyrazam), ring benzene pyrrole bacterium amine (sedaxane), fluxapyroxad (fluxapyroxad) and pyrrole metsulfovax (penthiopyrad).
Boscalid, English name:Cantus, boscalid, chemical name:The chloro- N- of 2- (4 '-chlorodiphenyl -2- bases) nicotinoyl Amine, chemical molecular formula:C18H12Cl2N2O, molecular weight:343.21, CAS:188425-85-6.Boscalid is by German BASF The Novel tobacco acid amide fungicides of company's exploitation, it is mainly used in preventing and treating powdery mildew, gray mold, various rots, brown rot and root Maize ear rot etc., Boscalid belong to butanedioic acid ubiquinone reductase inhibitor in mitochondrial respiratory chain, and the sprouting to spore has very strong Rejection ability, and with other bactericide no interactions resistances.Boscalid is permeated in plant by blade face to be shifted, and suppresses mitochondria Succinate dehydrogenase, tricarboxylic acid cycle is hindered, lack amino acid, sugar, energy reduction, the division and growth of interference cell, There is neural activity to disease, there is protection and therapeutic action.It is female to suppress spore germination, the extension of bacterium pipe, mycelial growth and spore Cell forms the Main Stage of fungi growth and breeding, and bactericidal action is directly caused by parent active agent, is not metabolized accordingly Activity.With the no interactions resistance such as carbendazim, Sukeling.
Mebenil, English name:Mepronil, also known as:Mebenil, mepronil, the third adjacent amine, line up to gram etc., chemical name: N- (3- isopropyl phenyls) -2- methyl benzamides, molecular formula:C17H19NO2, CAS:55814-41-0.Mebenil is a kind of Systemic fungicide, various crop Major Diseases can be effectively prevented and treated, and the term of validity is long, is not likely to produce poisoning, resistance of rainwater washing against, To having special efficacy by the fungus-caused crop disease of Basidiomycotina, germ can be prevented to invade host, play preventive and therapeutic action. Mebenil belongs to the succinate dehydrogenase for suppressing Complex II so as to hindering the breathing of pathogen.With suppression and prevent banded sclerotial blight Bacterium invades host, reaches preventive and therapeutic action, field control effectiveness test shows, has favorable effect to rice sheath blight disease.
Flutolanil, English name:Flutolanil, also known as:Line cumidine, Wang Jiaduo, fluorine line amine, fluorine mepronil, chemical name Claim:N- (3 '-isopropyl phenyl) -2- trifluoromethyl benzamides, molecular formula:C17H16F3NO2, molecular weight:323.31, CAS: 66332-96-5.Flutolanil category heterocyclic carbamate derivatives, there is the systemic fungicide of preventive and therapeutic action.Flutolanil belongs to Succinate dehydrogenase inhibitors, suppress the synthesis of aspartate and glutamate, and the medicament has protection and therapeutic action, energy Enough prevent the growth of pathogen and penetrate, disease caused by the main pathogen for preventing and treating Basidiomycota.Fertility to mycelia and invade The formation for entering mycelia block has very strong inhibition.The rice sheath blight disease as caused by preventing and treating rhizoctonia, with 50mg/L concentration stems Leaf processing, prevention effect 100%.
Banded sclerotial blight is also known as moire disease, and popular name spends sufficient stalk, rotten pin pest, features spot.It is to be fallen ill by Rhizoctonia solani Kuhn infection, exists more Occur under high temperature, super-humid conditions.Banded sclerotial blight causes harm seriously in South Rice Region of China, is one of the Major Diseases in current Rice Production. The disease prevents rice from earing, or the not plump paddy of heading is more, and grain declines again.
Powdery mildew, can be fallen ill from seedling to heading.Main crop leaf of causing harm, also cause harm stem and tassel.In blade On start produce yellow dot, then expand develop into circular or oval scab, there is the mould layer of white powder on surface.General feelings Condition lower blade is more than upper blade, and vacuum side of blade is more than front.Mildew early stage is individually scattered, after be unified into a big mildew, Full leaf even can be covered, photosynthesis is had a strong impact on, eubolism is interfered, cause early ageing, yield is damaged Lose
Frequent use containing single-activity component bactericide, most germs are caused to generate the resistance to the action of a drug, preventive effect is not It is preferable.Therefore, it is badly in need of the sterilised products that a kind of novel product substitutes single-activity component, increase drug effect delays the resistance to the action of a drug of disease Risk, reduce economic loss.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of component is reasonable, synergistic effect is notable, and bactericidal effect is good, medication Cost is low, is not likely to produce the resistance to the action of a drug, the bactericidal composition to crop safety and its application.
In order to solve the above technical problems, present invention offer technical scheme is as follows:
The bactericidal composition of the present invention, containing effective constituents A and active ingredient B, effective constituents A is shenqinmycin, effectively Composition B is succinate dehydrogenase inhibitors series bactericidal agent, effective constituents A shenqinmycin and the suppression of active ingredient B succinate dehydrogenases The weight ratio of preparation series bactericidal agent is 1:100~100:1, preferably weight ratio is 1:50~50:1, more preferably weight ratio is 1: 25~25:1.
In the bactericidal composition of the present invention, the active ingredient B succinate dehydrogenase inhibitors series bactericidal agent can be pyridine The one or more of acyl bacterium amine, mebenil or flutolanil.
Pesticidal preparations auxiliary element can also be contained in bactericidal composition of the present invention, be adapted to agriculturally use to be made Formulation.The pesticidal preparations auxiliary element is one kind in solvent, emulsifying agent, dispersant, stabilizer, antifreezing agent or wetting agent It is or several.
The bactericidal composition of the present invention can be prepared into any one formulation of suitable agricultural use with known method, than Preferable formulation be dry suspending agent (DF), wettable powder (WP), water dispersible granules (WDG), suspending agent (SC), oil-suspending agent, Solvable pulvis, granule, microemulsion, aqueous emulsion, microcapsule suspending agent etc..
A wettable powders (WP), be can be dispersed in water solid powder preparation.Wettable powder of the present invention except Into active compound plus such as outside white carbon, precipitated calcium carbonate, clay, diatomite, attapulgite diluent or inert substance, also from Subtype or nonionic surface active agent (wetting agent, dispersant), wetting agent is for example, alkylbenzenesulfonate (DBS-Na), alkyl Naphthalene sulfonate (pull open powder), alkylphenol-polyethenoxy base ether sulfate, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulfate, Aliphatic alcohol sulfate, fatty alcohol ethylene oxide adduct sulfonate, alkyl amide taurate, aliphatic alcohol polyethenoxy base ether (JFC), content 0.1~30%;Dispersant such as naphthalene sulfonate, naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), wood Quality and its derivative sulfonate (M-9, POLYFON etc.), polyoxyethylene poly-oxygen propylene aether block copolymer, alkyl phenol polyoxy second Alkene ether formaldehyde condensation products sulfate (SOPA), PVA, CMC content 0.1~30%;Also containing penetrating agent JFC, azone, thiophene ketone, One or more materials among penetrant t, content 0.3~30%;Stabilizer, foam inhibitor, anticaking agent etc. can also be contained Other auxiliary agents.
B water dispersible granules (WDG), be can be comparatively fast disintegrated in water, the scattered granule for forming higher suspension dispersion.By The key element such as the auxiliary agent such as active component, wetting agent, dispersant, interleaving agent, disintegrant, stabilizer, adhesive and carrier forms.It is helped Agent and carrier, and the compound method of precursor (preformed objects before granulation) are analogous to wettable powder and suspending agent substantially.
C suspending agents (SC), it is the not dispersion of water soluble solid agricultural chemicals or immiscible liquids agricultural chemicals in water or oil.By activity Composition, auxiliary agent (wetting dispersing agent, thickener, stabilizer, pH adjusting agent, defoamer, antifreezing agent) are made through soybean dietary fiber. Wetting dispersing agent can be naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), lignin and its derivative sulfonate (M-9, POLYFON etc.), alkylphenol-polyethenoxy base ether sulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, alkyl Phenol polyethenoxy base phosphate, carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), condensation phosphorus One or more in hydrochlorate etc., content 0.1~12%;Thickener and stabilizer can be xanthan gum (XG), polyvinyl alcohol (PVA), the one or more in Magnesiumaluminumsilicate etc., content 0.05~0.5%;PH adjusting agent and defoamer optionally can add in right amount Add.
D dry suspending agents (Dry Flowable, abbreviation DF), it is that can be comparatively fast disintegrated in water, scattered form higher suspension and disperse The granule of system.Preparation method:The preparation obtained by the dehydration of suspending agent.Its auxiliary agent and carrier, and precursor (dehydrate Preceding preformed objects) compound method it is consistent with suspending agent.
Bactericidal composition of the present invention is applied to prepare the pesticidal preparations of controlling plant diseases.Particularly useful for making Preventing and treating rice sheath blight disease, rice blast, false smut, wheat rust, powdery mildew, downy mildew, gray mold, epidemic disease, scab, spot fall Leaf disease, net blotch, glume blight, eyeprint disease, ring spot, droop, gray mold, anthracnose, canker, banded sclerotial blight, sclerotiniose Pesticidal preparations.
Bactericidal composition of the present invention can be provided in the form of finished product preparation, i.e., each component substances have been in bactericidal composition It is blended, or the composition of bactericidal composition can also provide with unit dose form, using it is preceding directly in bucket or tank it is directly mixed Close, be then diluted to required concentration.
Bactericidal composition of the present invention is applied to the method for controlling disease, and effective constituents A and composition B are with above-mentioned weight Than applying, it applies, is administered simultaneously in proportion or applied successively in proportion amount after can mixing in proportion.Specific method of application can be with It is powder injection process, spray-on process, smog method, fumigating system and seed treatment and soil treatment, ultra-low volume spray, mixes fertilizer, spread fertilizer over the fields, fill Root.
Compared with prior art, the beneficial effects of the present invention are:
1st, compared with single dose, compounding has obvious synergistic function, improves prevention effect;
2nd, composition is made up of two kinds of different active ingredients of mechanism of action, and bactericidal effect is good, is advantageous to overcome and delays The drug-fast generation of germ;
3rd, medicament mixture reduces dosage, so as to reduce cost and alleviate the pollution to environment.
Instant component is reasonable, and treatment plus protective effect, drug cost is low, and its activity and bactericidal effect are not each components The simple superposition of activity, but have significant synergistic effect, it is good to crop safety, meet the security requirement of pesticidal preparations.
Embodiment
To make the technical problem to be solved in the present invention, technical scheme and advantage clearer, specific embodiment will be used below It is described in detail, but the present invention is limited to absolutely not these examples.As described below is only the preferable embodiment of the present invention, only to The present invention is explained, therefore can not be interpreted as the limitation to the scope of the claims of the present invention.It is it should be pointed out that all the present invention's All any modification, equivalent and improvement done within spirit and principle etc., should be included in the scope of the protection. Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Recompounded pesticide is made in the combination of active principles of different agricultural chemicals, is current exploitation and develops novel pesticide and preventing and treating agriculture A kind of effective and quick way of resistance germ in industry.After the pestsides synthesis of different cultivars, a large amount of three kinds of works are typically exhibited Use type:Summation action, synergistic effect and antagonism.Compound synergic is formulated well, can significantly improve actual preventing and treating effect Fruit, the usage amount of agricultural chemicals is reduced, be the important hand of integrated control disease so as to help to delay the drug-fast generation speed of germ Section.
The present composition is by a kind of active ingredient (A), succinate dehydrogenase inhibitors series bactericidal agent of shenqinmycin Another active ingredient (B), between them combination there is obvious synergistic function to plant disease, rather than just two kinds of medicines The simple superposition of agent effect.
In order to prevent and treat the gray mold of cucumber in agricultural production, the increasing that inventor is compounded with shenqinmycin and Boscalid Effect research, specific method are:
Experiment uses gray mold of cucumber as test object.
The test medicine that active compound is configured to need, test method:Fungicide bioassay pattern -- cotyledon method, reference 《People's Republic of China's agricultural industry criteria》.Selection broad bean susceptible variety is potted plant, and clip same section, growing way is consistent, carries The blade of petiole, is put in culture dish, and moisturizing is standby, and experimental pharmacy is used into active compound water dissolved dilution, sets 5 quality dense Degree, by blade in pre-configured good decoction fully infiltration 5S, drain unnecessary decoction, after natural air drying, protected after being marked by processing Wet culture, experiment set the processing without medicament as blank control.The one side that a diameter of 5mm bacteria cakes have mycelia is inoculated with inoculator In processing central vane, 30 leaves of each processing inoculation.Protectiveness experiment 24h after chemicals treatment is inoculated with, and curative tests exist 24h is inoculated with before chemicals treatment.Growth cabinet or constant temperature are placed in after inoculation to be had in illumination humidistat in fact, 26 degrees Celsius of temperature- 28 degrees Celsius, cultivate under conditions of relative humidity 80%-90%.According to blank control incidence, recorded with kind of calliper every Individual vaccination scab length and width, represent that lesion diameter records lesion diameter (unit mm) according to investigation with length and width average value Data, by preventing and treating result publicity:Preventive effect=(blank diameter-processing diameter)/blank diameter * 100, calculate each processing and suppress scab The percentage of growth, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain each medicine The EC50 values of agent, the co-toxicity coefficient (CTC) of mixture is calculated with co-toxicity coefficient meter, work of the reagent agent to germ is evaluated with this Property.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect;CTC≤80 show as antagonism;80 ﹤ CTC ﹤ 120 show as summation action.
The shenqinmycin of table 1 and Boscalid compound the Toxicity Determination to gray mold of cucumber
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shenqinmycin (A) —— Y=4.8588+0.3241X 2.7261
Boscalid (B1) —— Y=3.8338+1.0540X 12.7753
A:B1 1:100 Y=4.2665+0.8040X 8.1689 150.88
A:B1 1:50 Y=4.3814+0.7464X 6.7383 177.18
A:B1 1:25 Y=4.5337+0.6124X 5.7710 193.91
A:B1 1:1 Y=4.6515+1.0223X 2.1921 204.98
A:B1 25:1 Y=4.6810+1.2596X 1.7913 156.94
A:B1 50:1 Y=4.6582+1.2264X 1.8994 145.786
A:B1 100:1 Y=4.5830+1.2780X 2.1194 129.64
Toxicity Determination result is shown, in weight than 1:100-100:In the range of 1, particularly 1:50-50:In the range of 1, Especially 1:25-25:In the range of 1, shenqinmycin has obvious synergistic effect to gray mold of cucumber with Boscalid compounding, prevents Control excellent effect and be better than single dose kind.
In order to prevent and treat the rice sheath blight disease in agricultural production, the synergy that inventor is compounded with shenqinmycin and mebenil Research, specific method are:
Experiment uses rice sheath blight disease as test object.
The test medicine that active compound is configured to need, test method (in vitro culture method) reference《People's Republic of China's agriculture Industry professional standard》.First by single dose and each Mixed Pharmacy set 5 various concentrations gradients (on the basis of pilot study result, Difference is combined and matched according to different agents, concentration setting is also different, and bacteriostasis rate is set in the range of 5%~95%). By medicament in the decoction prepared in advance fully infiltration 5s, unnecessary decoction is drained, by each processing blade and blade after natural air drying Upper backwards, standby in moisture preservation box by being emitted on after processing mark, experiment sets the processing without medicament as blank control.Use inoculator The one side that the sheath blight fungus prepared has mycelia is inoculated in rice leaf center, 30 leaves of often processing inoculation, protectiveness experiment 24h is inoculated with after chemicals treatment, and curative tests 24h before chemicals treatment is inoculated with, and temperature is placed in after inoculation as 26~28 DEG C, wet Spend in the growth cabinet for 80%~90% and cultivate;According to blank control incidence, lesion diameter is recorded, calculates each processing Suppress the percentage of scab growth, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, The EC50 values of each medicament are obtained, the co-toxicity coefficient (CTC) of mixture is calculated with co-toxicity coefficient meter, reagent agent pair is evaluated with this The activity of germ.The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect;CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
The shenqinmycin of table 2 and mebenil compound the Toxicity Determination to rice sheath blight disease
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shenqinmycin (A) —— Y=4.3775+0.6081X 10.5548 ——
Mebenil (B2) —— Y=3.5893+0.9992X 25.8087 ——
A:B2 1:100 Y=3.8688+0.8970X 18.2398 139.50
A:B2 1:50 Y=3.8864+0.9541X 14.6907 170.84
A:B2 1:25 Y=3.8458+1.0379X 12.9395 188.96
A:B2 1:1 Y=3.7448+1.5318X 6.5976 227.08
A:B2 25:1 Y=3.6606+1.5900X 6.9557 155.27
A:B2 50:1 Y=3.7041+1.5011X 7.2987 147.97
A:B2 100:1 Y=3.7977+1.3299X 8.0169 132.44
Inventor has found by test, in weight than 1:100-100:In the range of 1, particularly 1:50-50:In the range of 1, especially It is 1:25-25:In the range of 1, shenqinmycin compounds with mebenil, there is the similar effect of obvious synergistic effect to rice sheath blight disease Fruit, there is obvious Complex synergism to plant disease.
In order to prevent and treat the powdery mildew of cucumber in agricultural production, the synergy that inventor is compounded with shenqinmycin and flutolanil Research, specific method are:
Experiment uses powdery mildew of cucumber as test object.
The test medicine that active compound is configured to need, test method (pot-culture method) reference《People's Republic of China's agricultural row Industry standard》.First by single dose and each Mixed Pharmacy set 5 various concentrations gradients (on the basis of pilot study result, according to Different agents combine and proportioning is different, and concentration setting is also different, and bacteriostasis rate is set in the range of 5%~95%).With spray Mist method is by medicine liquid spray on standby wheat seedling, and standby after naturally dry, experiment sets the processing without medicament as blank control.Will The caused fresh sporozoite of Powdery Mildew uniformly tiltedly falls to be inoculated in the 2-3 leaf phases potted plant wheat of processing in 24h on morbidity wheat leaf blade Miao Shang, often handle 3 basins.Protectiveness experiment 24h after chemicals treatment, curative tests 24h before chemicals treatment are inoculated with, then put 28 degrees Celsius of constant temperature lighting box cultures.According to blank control incidence, lesion diameter is recorded, each processing is calculated and suppresses scab growth Percentage, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain each medicament EC50 values, the co-toxicity coefficient (CTC) of mixture is calculated with co-toxicity coefficient meter, activity of the reagent agent to germ is evaluated with this. The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect;CTC≤80 show as antagonism;The tables of 80 ﹤ CTC ﹤ 120 It is now summation action.
The shenqinmycin of table 3 and flutolanil compound the Toxicity Determination to powdery mildew of cucumber
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shenqinmycin (A) —— Y=3.5870+0.9381X 32.0653 ——
Flutolanil (B3) —— Y=3.5893+0.9992X 25.8087 ——
A:B3 1:100 Y=3.8754+0.8749X 19.2868 134.07
A:B3 1:50 Y=3.9176+0.9388X 14.2183 182.21
A:B3 1:25 Y=3.9476+0.9325X 13.4435 193.42
A:B3 1:1 Y=3.9632+0.9258X 13.1762 217.04
A:B3 25:1 Y=3.8590+0.9353X 16.5893 191.50
A:B3 50:1 Y=3.7756+0.9132X 21.9102 145.65
A:B3 100:1 Y=3.7386+0.9197X 23.5185 136.01
Toxicity Determination result is shown, in weight than 1:100-100:In the range of 1, particularly 1:50-50:In the range of 1, Especially 1:25-25:In the range of 1, shenqinmycin has obvious synergistic effect to powdery mildew of cucumber with flutolanil compounding, prevents and treats Excellent effect and it is better than single dose kind.
The bactericidal composition of the present invention can be prepared into any one formulation of suitable agricultural use with known method, than Preferable formulation is wettable powder, aqueous suspension agent, oil-suspending agent, suspended emulsion, water dispersible granules.Percentage in all formulas It is weight percentage.The processing technology of the various formulations of the present composition is prior art, can be had according to different situations Changed.
To make the technical problem to be solved in the present invention, technical scheme and advantage clearer, in weight than 1:100~100: In the range of 1, embodiment of the shenqinmycin respectively with Boscalid, mebenil or flutolanil compounding is built, is described in detail, but The present invention is limited to absolutely not these examples.
In addition, inventor is also found through experiments that, the shenqinmycin compounding with Boscalid, mebenil or flutolanil respectively Effect, better than shenqinmycin and the compounding effect of other succinate dehydrogenase inhibitors series bactericidal agents.In order to clearly demonstrate this Individual problem, in weight than 1:100~100:In the range of 1, structure shenqinmycin is sterilized with other succinate dehydrogenase inhibitors classes The comparative example of agent, is described in detail.But because length is limited, representative several comparative examples can only be enumerated.It should refer to Go out, the shenqinmycin compounding effect with Boscalid, mebenil or flutolanil respectively, better than shenqinmycin and other ambers The compounding effect of dehydrogenase inhibitor series bactericidal agent, it is not limited in limited comparative example.
Present invention is described further with reference to embodiment, comparative example.Embodiment comparative example
One:Shenqinmycin and Boscalid
Embodiment 1:30.3% shenqinmycin Boscalid suspending agent (1:100)
Component:Shenqinmycin 0.3%, Boscalid 30%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 5%, poly alkyl alcohol Oxygen vinethene 3%, ethylene glycol 6%, XG0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.6%, soft water complements to 100%.
Preparation method:Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down Equal particle diameter reaches 1-5 microns, you can 30.3% shenqinmycin Boscalid suspending agent is made.
Comparative example 1:30.3% shenqinmycin thifluzamide suspending agent (1:100)
Component:Shenqinmycin 0.3%, thifluzamide 30%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 5%, poly alkyl alcohol Oxygen vinethene 3%, ethylene glycol 6%, XG0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.6%, soft water complements to 100%.
Preparation method:Ibid.
Embodiment 2:33.8% shenqinmycin Boscalid water dispersible granules (1:83.5)
Component:Shenqinmycin 0.4%, Boscalid 33.4%, polycarboxylic acids sodium 3%, sodium lignin sulfonate 6%, anhydrous sulphur Sour sodium 8%, kaolin complements to 100%.
Preparation method:Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 33.8% shenqinmycin Boscalid water dispersible granules are made.
Comparative example 2:33.8% jinggangmeisu Boscalid water dispersible granules (1:83.5)
Component:Jinggangmeisu 0.4%, Boscalid 33.4%, polycarboxylic acids sodium 3%, sodium lignin sulfonate 6%, anhydrous sulphur Sour sodium 8%, kaolin complements to 100%.
Preparation method:Ibid.
Embodiment 3:30.6% shenqinmycin Boscalid dry suspending agent (1:50)
Component:Shenqinmycin 0.6%, Boscalid 30%, polycarboxylic acids sodium 4%, sodium lignin sulfonate 8%, white carbon 8%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method:The water of 1.5-2.0 times of preparation amount of preparation is added, each component is mixed, is mixed through high speed shear equal It is even, then 2-3 hours are sanded by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 30.6% Shen piperazine mould Plain Boscalid dry suspending agent.
Comparative example 3:30.6% shenqinmycin thifluzamide dry suspending agent (1:50)
Component:Shenqinmycin 0.6%, thifluzamide 30%, polycarboxylic acids sodium 4%, sodium lignin sulfonate 8%, white carbon 8%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method:Ibid.
Embodiment 4:35% shenqinmycin Boscalid dry suspending agent (1:34)
Component:Shenqinmycin 1%, Boscalid 34%, lignin sodium naphthalene sulfonate 8%, sodium lignin sulfonate 8%, hard charcoal Black 8%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method:The water of 1.5-2.0 times of preparation amount of preparation is added, each component is mixed, is mixed through high speed shear equal It is even, then 2-3 hours are sanded by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 34% Shen piperazine mould Plain Boscalid dry suspending agent.
Comparative example 4:35% polyoxin Boscalid dry suspending agent (1:34)
Component:Polyoxin 1%, Boscalid 34%, lignin sodium naphthalene sulfonate 8%, sodium lignin sulfonate 8%, hard charcoal Black 8%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method:Ibid.
Embodiment 5:26% shenqinmycin Boscalid water dispersible granules (1:25)
Component:Shenqinmycin 1%, Boscalid 25%, polycarboxylic acids sodium 3%, sodium naphthalene sulfonate 5%, soluble starch 6%, Kaolin complements to 100%.
Preparation method:Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 26% shenqinmycin Boscalid water dispersible granules are made.
Comparative example 5:26% shenqinmycin thifluzamide water dispersible granule (1:25)
Component:Shenqinmycin 1%, thifluzamide 25%, polycarboxylic acids sodium 3%, sodium naphthalene sulfonate 5%, soluble starch 6%, Kaolin complements to 100%.
Preparation method:Ibid.
Embodiment 6:20% shenqinmycin Boscalid dry suspending agent (1:1)
Component:Shenqinmycin 10%, Boscalid 10%, lignin sodium naphthalene sulfonate 6%, sodium lignin sulfonate 6%, hard charcoal Black 6%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method:The water of 1.5-2.0 times of preparation amount of preparation is added, each component is mixed, is mixed through high speed shear equal It is even, then 2-3 hours are sanded by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 20% Shen piperazine mould Plain Boscalid dry suspending agent.
Comparative example 6:20% shenqinmycin thifluzamide dry suspending agent (1:1)
Component:Shenqinmycin 10%, thifluzamide 10%, lignin sodium naphthalene sulfonate 6%, sodium lignin sulfonate 6%, hard charcoal Black 6%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method:Ibid.
Comparative example 7:20% kasugarnycin Boscalid dry suspending agent (1:1)
Component:Kasugarnycin 10%, Boscalid 10%, lignin sodium naphthalene sulfonate 6%, sodium lignin sulfonate 6%, hard charcoal Black 6%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method:Ibid.
Embodiment 7:26% shenqinmycin Boscalid suspending agent (25:1)
Component:Shenqinmycin 25%, Boscalid 1%, polycarboxylic acids sodium 2%, AEO 4%, 1,2- third Glycol 5%, XG0.33%, organic silicon defoamer 0.2%, sodium benzoate 0.6%, soft water complements to 100%.
Preparation method:Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down Equal particle diameter reaches 1-5 microns, you can 26% shenqinmycin Boscalid suspending agent is made.
Embodiment 8:25.6% shenqinmycin Boscalid wettable powder (31:1)
Component:Shenqinmycin 24.8%, Boscalid 0.8%, lauryl sodium sulfate 4%, sodium naphthalene sulfonate 6%, hard charcoal Black 10%, precipitated calcium carbonate complements to 100%.
Preparation method:Each component is well mixed, first passes through mechanical crushing, then after air-flow crushing, is well mixed, i.e., 25.6% shenqinmycin Boscalid wettable powder can be made.
Comparative example 8:25.6% polyoxin Boscalid wettable powder (31:1)
Component:Polyoxin 24.8%, Boscalid 0.8%, lauryl sodium sulfate 4%, sodium naphthalene sulfonate 6%, hard charcoal Black 10%, precipitated calcium carbonate complements to 100%.
Preparation method:Ibid.
Embodiment 9:32.4% shenqinmycin Boscalid suspending agent (80:1)
Component:Shenqinmycin 32%, Boscalid 0.4%, polycarboxylic acids sodium 2%, OPEO 4%, second two Alcohol 6%, XG 0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.6%, soft water complements to 100%.
Preparation method:Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down Equal particle diameter reaches 1-5 microns, you can 32.4% shenqinmycin Boscalid suspending agent is made.
Comparative example 9:32.4% shenqinmycin thifluzamide suspending agent (80:1)
Component:Shenqinmycin 32%, thifluzamide 0.4%, polycarboxylic acids sodium 2%, OPEO 4%, second two Alcohol 6%, XG 0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.6%, soft water complements to 100%.
Preparation method:Ibid.
2nd, shenqinmycin and mebenil
Embodiment 10:40.4% shenqinmycin mebenil suspending agent (1:100)
Component:Shenqinmycin 0.4%, mebenil 40%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 5%, octyl phenol polyoxy Vinethene sodium sulfonate 3%, 1,2-PD 5%, XG0.25%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water are mended Enough to 100%.
Preparation method:Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down Equal particle diameter reaches 1-5 microns, you can 40.4% shenqinmycin mebenil suspending agent is made.
Comparative example 10:40.4% tetramycin mebenil suspending agent (1:100)
Component:Tetramycin 0.4%, mebenil 40%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 5%, octyl phenol polyoxy Vinethene sodium sulfonate 3%, 1,2-PD 5%, XG0.25%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water are mended Enough to 100%.
Preparation method:Ibid.
Embodiment 11:33.3% shenqinmycin mebenil wettable powder (1:65.6)
Component:Shenqinmycin 0.5%, mebenil 32.8%, neopelex 3%, lignin sulfonic acid sodium formaldehyde Condensation product 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.
Preparation method:Each component is well mixed, first passes through mechanical crushing, then after air-flow crushing, is well mixed, i.e., 33.3% shenqinmycin mebenil wettable powder can be made.
Comparative example 11:33.3% shenqinmycin thifluzamide wettable powder (1:65.6)
Component:Shenqinmycin 0.5%, thifluzamide 32.8%, neopelex 3%, sodium lignin sulfonate first Aldehyde condensate 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.
Preparation method:Ibid.
Embodiment 12:36% shenqinmycin mebenil wettable powder (1:35)
Component:Shenqinmycin 1%, mebenil 35%, neopelex 4%, sodium naphthalene sulfonate 8%, white carbon 8%, kaolin complements to 100%.
Preparation method:Each component is well mixed, first passes through mechanical crushing, then after air-flow crushing, is well mixed, i.e., 36% shenqinmycin mebenil wettable powder can be made.
Comparative example 12:36% shenqinmycin thifluzamide wettable powder (1:35)
Component:Shenqinmycin 1%, thifluzamide 35%, neopelex 4%, sodium naphthalene sulfonate 8%, white carbon 8%, kaolin complements to 100%.
Preparation method:Ibid.
Embodiment 13:26% shenqinmycin mebenil suspending agent (25:1)
Component:Shenqinmycin 25%, mebenil 1%, polycarboxylic acids sodium 3%, AEO 3%, ethylene glycol 6%, XG0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.6%, soft water complements to 100%.
Preparation method:Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down Equal particle diameter reaches 1-5 microns, you can 26% shenqinmycin mebenil suspending agent is made.
Comparative example 13:26% shenqinmycin thifluzamide suspending agent (25:1)
Component:Shenqinmycin 25%, thifluzamide 1%, polycarboxylic acids sodium 3%, AEO 3%, ethylene glycol 6%, XG0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.6%, soft water complements to 100%.
Preparation method:Ibid.
Embodiment 14:25.5% shenqinmycin mebenil water dispersible granules (50:1)
Component:Shenqinmycin 25%, mebenil 0.5%, lignin sodium naphthalene sulfonate 3%, polycarboxylic acids sodium 4%, anhydrous slufuric acid Sodium 8%, kaolin complements to 100%.
Preparation method:Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 25.5% shenqinmycin mebenil water dispersible granules are made.
Comparative example 14:25.5% tetramycin mebenil water dispersible granules (50:1)
Component:Tetramycin 25%, mebenil 0.5%, lignin sodium naphthalene sulfonate 3%, polycarboxylic acids sodium 4%, anhydrous slufuric acid Sodium 8%, kaolin complements to 100%.
Preparation method:Ibid
Embodiment 15:20.2% shenqinmycin mebenil dry suspending agent (100:1)
Component:Shenqinmycin 20%, mebenil 0.2%, lignin sodium naphthalene sulfonate 6%, sodium lignin sulfonate 6%, hard charcoal Black 10%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method:The water of 1.5-2.0 times of preparation amount of preparation is added, each component is mixed, is mixed through high speed shear equal It is even, then 2-3 hours are sanded by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 20.2% Shen piperazine mould Plain mebenil dry suspending agent.
Comparative example 15:20.2% shenqinmycin thifluzamide microcapsule suspending agent (100:1)
Component:Shenqinmycin 20%, thifluzamide 0.2%, lignin sodium naphthalene sulfonate 6%, sodium lignin sulfonate 6%, in vain Carbon black 10%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method:Ibid.
3rd, shenqinmycin and flutolanil
Embodiment 16:40.4% shenqinmycin flutolanil suspending agent (1:100)
Component:Shenqinmycin 0.4%, flutolanil 40%, polycarboxylic acids sodium 4%, OPEO phosphate 2%, Ethylene glycol 6%, XG0.25%, organic silicon defoamer 0.2%, sodium benzoate 0.6%, soft water complements to 100%.
Preparation method:Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down Equal particle diameter reaches 1-5 microns, you can 40.4% shenqinmycin flutolanil suspending agent is made.
Comparative example 16:40.4% polyoxin flutolanil suspending agent (1:100)
Component:Polyoxin 0.4%, flutolanil 40%, polycarboxylic acids sodium 4%, OPEO phosphate 2%, Ethylene glycol 6%, XG0.25%, organic silicon defoamer 0.2%, sodium benzoate 0.6%, soft water complements to 100%.
Preparation method:Ibid.
Embodiment 17:46% shenqinmycin flutolanil wettable powder (1:45)
Component:Shenqinmycin 1%, flutolanil 45%, neopelex 4%, lignin sodium naphthalene sulfonate 8%, in vain Carbon black 8%, precipitated calcium carbonate complements to 100%.
Preparation method:Each component is well mixed, first passes through mechanical crushing, then after air-flow crushing, is well mixed, i.e., 46% shenqinmycin flutolanil wettable powder can be made.
Comparative example 17:46% shenqinmycin thifluzamide wettable powder (1:45)
Component:Shenqinmycin 1%, thifluzamide 45%, neopelex 4%, lignin sodium naphthalene sulfonate 8%, White carbon 8%, precipitated calcium carbonate complements to 100%.
Preparation method:Ibid.
Embodiment 18:20% shenqinmycin flutolanil dry suspending agent (1:1)
Component:Shenqinmycin 10%, flutolanil 10%, lignin sodium naphthalene sulfonate 6%, sodium lignin sulfonate 6%, white carbon 8%, organic silicon defoamer 0.2%, sodium benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method:The water of 1.5-2.0 times of preparation amount of preparation is added, each component is mixed, is mixed through high speed shear equal It is even, then 2-3 hours are sanded by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 20% Shen piperazine mould Plain flutolanil dry suspending agent.
Comparative example 18:20% jinggangmeisu flutolanil dry suspending agent (1:1)
Component:Jinggangmeisu 10%, flutolanil 10%, lignin sodium naphthalene sulfonate 6%, sodium lignin sulfonate 6%, white carbon 8%, organic silicon defoamer 0.2%, sodium benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method:Ibid.
Embodiment 19:25.5% shenqinmycin flutolanil water dispersible granules (50:1)
Component:Shenqinmycin 25%, flutolanil 0.5%, lignin sodium naphthalene sulfonate 6%, polycarboxylic acids sodium 4%, anhydrous slufuric acid Sodium 8%, kaolin complements to 100%.
Preparation method:Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 25.5% shenqinmycin flutolanil water dispersible granules are made.
Comparative example 19:25.5% shenqinmycin thifluzamide water dispersible granule (50:1)
Component:Shenqinmycin 25%, thifluzamide 0.5%, lignin sodium naphthalene sulfonate 6%, polycarboxylic acids sodium 4%, anhydrous sulphur Sour sodium 8%, kaolin complements to 100%.
Preparation method:Ibid.
Embodiment 20:20.2% shenqinmycin flutolanil suspending agent (100:1)
Component:Shenqinmycin 20%, flutolanil 0.2%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinethene 3%, ethylene glycol 5%, XG0.35%, organic silicon defoamer 0.3%, sodium benzoate 0.6%, soft water complements to 100%.
Preparation method:Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down Equal particle diameter reaches 1-5 microns, you can 20.2% shenqinmycin flutolanil suspending agent is made.
Comparative example 20:20.2% kasugarnycin flutolanil suspending agent (100:1)
Component:Kasugarnycin 20%, flutolanil 0.2%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinethene 3%, ethylene glycol 5%, XG0.35%, organic silicon defoamer 0.3%, sodium benzoate 0.6%, soft water complements to 100%.
Preparation method:Ibid.
Found by experiment, Boscalid, mebenil, the flutolanil in above example can be replaced arbitrarily, new to be formed Embodiment.This is no longer carried out to repeat to enumerate, it is noted that for those skilled in the art, not On the premise of departing from principle of the present invention, some improvements and modifications can also be made, these improvements and modifications also should be regarded as this The protection domain of invention.
Pesticidal preparations preventing and treating graw mold of tomato, sclerotinia sclerotiorum experiment made from above-described embodiment, comparative example.
1st, test process:Experiment is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination Test dosing and three concentration for the treatment of are set according to the difference of each composition respectively, comparison medicament is the commercially available pyridine acyl of agricultural chemicals 50% respectively Bacterium aqueous amine dispersible granule, 1% shenqinmycin SC and blank clean water experiment.
2nd, test method:Each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after investigating and preventing and treating before dispenser Method is:It is classified and is investigated with blank control incidence, per the sampling of cell diagonal, often clicks 10 plants, record total strain number, diseased plant number With sick series.
Using following stage division:
0 grade:It is disease-free;
1 grade:Lesion area accounts for less than the 5% of whole leaf area;
3 grades:Lesion area accounts for the 6%-10% of whole leaf area;
5 grades:Lesion area accounts for the 11%-20% of whole leaf area;
7 grades:Lesion area accounts for the 21%-40% of whole leaf area;
9 grades:Lesion area accounts for more than the 41% of whole leaf area;
Result of the test see the table below 4,5:
The shenqinmycin of table 4 (A) and Boscalid (B1) compound the prevention effect to graw mold of tomato
The field efficacy result of table 4 shows, in weight than 1:100-100:In the range of 1, shenqinmycin compounds with Boscalid Synergistic effect is obvious, and particularly 1:50-50:In the range of 1, especially 1:25-25:In the range of 1, have to graw mold of tomato excellent Prevention effect, prevention effect is better than single dose kind.
The embodiment of table 5 is compared with comparative example is to the prevention effect of sclerotinia sclerotiorum
Inventor has found that other antibioticses bactericide compound with Boscalid, in weight than 1:100-100:In the range of 1 Also there is certain Complex synergism.Because length is limited, do not enumerate, only with most representational jinggangmeisu, spring thunder Mycin, polyoxin construct comparative example and embodiment and carry out preventive effect contrast, as the field efficacy result of table 5 shows, in weight ratio 1:100-100:In the range of 1, shenqinmycin and Boscalid Complex synergism are obvious, particularly 1:50-50:In the range of 1, 1:25-25:In the range of 1, the prevention effect synergy to plant disease is more obvious, and shenqinmycin is imitated with Boscalid compound synergic Fruit is better than other antibioticses bactericide and the prevention effect of Boscalid compounding.
Other butanedioic acids suppress series bactericidal agent and compounded with shenqinmycin, in weight than 1:100-100:Also have in the range of 1 Certain Complex synergism.Because length is limited, do not enumerate, comparative example is only constructed with most representational thifluzamide Preventive effect contrast is carried out with embodiment, as the field efficacy result of table 5 shows, in weight than 1:100-100:In the range of 1, shenqinmycin It is obvious with Boscalid Complex synergism, particularly 1:50-50:In the range of 1,1:25-25:In the range of 1, to plant disease Preventing and treating synergy it is more obvious, shenqinmycin and Boscalid Complex synergism be better than other butanedioic acids suppress series bactericidal agents with The prevention effect of shenqinmycin compounding.
Pesticidal preparations preventing and treating rust of pear, root rotof flax experiment made from above-described embodiment, comparative example.
1st, test process:Experiment is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination Test dosing and three concentration for the treatment of are set according to the difference of each composition respectively, comparison medicament is that commercially available agricultural chemicals 75%% goes out respectively Become rusty amine wp, 1% shenqinmycin SC and blank clean water experiment.
2nd, test method:Each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after investigating and preventing and treating before dispenser Method is:It is classified and is investigated with blank control incidence, per the sampling of cell diagonal, often clicks 10 plants, record total strain number, diseased plant number With sick series.
Using following stage division:
0 grade:It is disease-free;
1 grade:Lesion area accounts for less than the 5% of whole leaf area;
3 grades:Lesion area accounts for the 6%-10% of whole leaf area;
5 grades:Lesion area accounts for the 11%-20% of whole leaf area;
7 grades:Lesion area accounts for the 21%-40% of whole leaf area;
9 grades:Lesion area accounts for more than the 41% of whole leaf area;
Result of the test see the table below 6,7:
The shenqinmycin of table 6 (A) and mebenil (B2) compound the prevention effect to rust of pear
The field efficacy result of table 6 shows, in weight than 1:100-100:In the range of 1, shenqinmycin increases with mebenil compounding Imitate positive effect, particularly 1:50-50:In the range of 1, especially 1:25-25:In the range of 1, there is excellent preventing and treating to rust of pear Effect, prevention effect are better than single dose kind.
The embodiment of table 7 is compared with comparative example is to the prevention effect of root rotof flax
Inventor has found that other antibioticses bactericide compound with mebenil, in weight than 1:100-100:In the range of 1 With certain Complex synergism.Because length is limited, do not enumerate, only constructed pair with most representational tetramycin Ratio and embodiment carry out preventive effect contrast, as the field efficacy result of table 7 shows, in weight than 1:100-100:In the range of 1, Shen piperazine Mycin and mebenil Complex synergism are obvious, particularly 1:50-50:In the range of 1,1:25-25:In the range of 1, to phytopathy Harmful prevention effect synergy is more obvious, shenqinmycin and mebenil Complex synergism be better than other antibioticses bactericide with The prevention effect of mebenil compounding.
Other butanedioic acids suppress series bactericidal agent and compounded with shenqinmycin, in weight than 1:100-100:Also have in the range of 1 Certain Complex synergism.Because length is limited, do not enumerate, comparative example is only constructed with most representational thifluzamide Preventive effect contrast is carried out with embodiment, as the field efficacy result of table 7 shows, in weight than 1:100-100:In the range of 1, shenqinmycin It is obvious with mebenil Complex synergism, particularly 1:50-50:In the range of 1,1:25-25:In the range of 1, to plant disease Prevention effect synergy is more obvious, shenqinmycin and mebenil Complex synergism be better than other butanedioic acids suppress series bactericidal agents with The prevention effect of shenqinmycin compounding.
Pesticidal preparations preventing and treating rice sheath blight disease, rice seedling blight experiment made from above-described embodiment, comparative example.
1st, test process:Experiment is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination Test dosing and three concentration for the treatment of are set according to the difference of each composition respectively, comparison medicament is the commercially available fluorine acyl of agricultural chemicals 20% respectively Amine WP, 1% shenqinmycin suspending agent and blank clean water experiment.
2nd, test method:Each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after investigating and preventing and treating before dispenser Method is:It is classified and is investigated with blank control incidence, per the sampling of cell diagonal, often clicks 10 plants, record total strain number, diseased plant number With sick series.
Using following stage division:
0 grade:It is disease-free;
1 grade:Lesion area accounts for less than the 5% of whole leaf area;
3 grades:Lesion area accounts for the 6%-10% of whole leaf area;
5 grades:Lesion area accounts for the 11%-20% of whole leaf area;
7 grades:Lesion area accounts for the 21%-40% of whole leaf area;
9 grades:Lesion area accounts for more than the 41% of whole leaf area;
Result of the test see the table below 8,9:
The shenqinmycin of table 8 (A) and flutolanil (B3) compound the prevention effect to rice sheath blight disease
The field efficacy result of table 8 shows, in weight than 1:100-100:In the range of 1, shenqinmycin increases with flutolanil compounding Imitate positive effect, particularly 1:50-50:In the range of 1, especially 1:25-25:In the range of 1, have to rice sheath blight disease excellent Prevention effect, prevention effect are better than single dose kind.
The embodiment of table 9 is compared with comparative example is to the prevention effect of rice seedling blight
Inventor has found that other antibioticses bactericide compound with flutolanil, in weight than 1:100-100:In the range of 1 With certain Complex synergism.Because length is limited, do not enumerate, only with most representational tetramycin, more anti-mildews Element, jinggangmeisu, kasugarnycin construct comparative example and embodiment and carry out preventive effect contrast, as the field efficacy result of table 9 shows, Weight is than 1:100-100:In the range of 1, shenqinmycin and flutolanil Complex synergism are obvious, particularly 1:50-50:1 model In enclosing, 1:25-25:More obvious to the prevention effect synergistic effect of plant disease in the range of 1, shenqinmycin is answered with flutolanil With the prevention effect for being better than other antibioticses bactericide and flutolanil compounding.
Other butanedioic acids suppress series bactericidal agent and compounded with shenqinmycin, in weight than 1:100-100:Also have in the range of 1 Certain Complex synergism.Because length is limited, do not enumerate, comparative example is only constructed with most representational thifluzamide Preventive effect contrast is carried out with embodiment, as the field efficacy result of table 9 shows, in weight than 1:100-100:In the range of 1, shenqinmycin It is obvious with flutolanil Complex synergism, particularly 1:50-50:In the range of 1,1:25-25:In the range of 1, to plant disease Prevention effect synergistic effect is more obvious, and shenqinmycin is better than other butanedioic acids with flutolanil compounding and suppresses series bactericidal agent and Shen piperazine The prevention effect of mycin compounding.
Inventor is had found using succinate dehydrogenase inhibitors series bactericidal agent as in Boscalid, mebenil, flutolanil Any one is compounded with shenqinmycin, in weight than 1:100-100:In the range of 1, particularly 1:50-50:In the range of 1, especially 1:25-25:In the range of 1, to downy mildew, gray mold, epidemic disease, late blight, powdery mildew, scab, spot defoliation, net blotch, The phytopathies such as glume blight, eyeprint disease, ring spot, brown spot, droop, rice blast, anthracnose, canker, banded sclerotial blight, sclerotiniose Evil also has obvious synergistic effect.Because length is limited, it is not repeated.
Described above is the preferred embodiment of the present invention, it is noted that for those skilled in the art For, on the premise of principle of the present invention is not departed from, some improvements and modifications can also be made, these improvements and modifications It should be regarded as protection scope of the present invention.

Claims (9)

1. a kind of bactericidal composition, it is characterised in that active ingredient contains shenqinmycin and succinate dehydrogenase inhibitors class is killed Microbial inoculum, the succinate dehydrogenase inhibitors series bactericidal agent are Boscalid, and the shenqinmycin and succinate dehydrogenase suppress The weight ratio of agent series bactericidal agent is 1:100~100:1.
2. bactericidal composition according to claim 1, it is characterised in that the shenqinmycin and succinate dehydrogenase suppress The weight ratio of agent series bactericidal agent is 1:50~50:1.
3. bactericidal composition according to claim 2, it is characterised in that the shenqinmycin and succinate dehydrogenase suppress The weight ratio of agent series bactericidal agent is 1:25~25:1.
4. according to any described bactericidal composition in claims 1 to 3, it is characterised in that also contain in the bactericidal composition There is pesticidal preparations auxiliary element, so that the formulation for being adapted to agriculturally use is made.
5. bactericidal composition according to claim 4, it is characterised in that the pesticidal preparations auxiliary element is solvent, breast One or more in agent, dispersant, stabilizer, antifreezing agent or wetting agent.
6. bactericidal composition according to claim 4, it is characterised in that the formulation of the bactericidal composition be suspending agent, Water dispersible granules, wettable powder, oil-suspending agent, solvable pulvis or granule.
7. application of any described bactericidal composition of claim 1 to 6 in the pesticidal preparations for preparing controlling plant diseases.
8. the application of bactericidal composition according to claim 7, it is characterised in that prevent and treat downy mildew, ash applied to preparing Mildew, epidemic disease, late blight, powdery mildew, scab, spot defoliation, net blotch, glume blight, eyeprint disease, ring spot, brown spot, Droop, rice blast, anthracnose, canker, banded sclerotial blight, the pesticidal preparations of sclerotiniose.
9. any described bactericidal composition of claim 1 to 6 is applied to the method for controlling disease, it is characterised in that effectively into Point shenqinmycin and succinate dehydrogenase inhibitors series bactericidal agent are applied with above-mentioned weight ratio, it applies after mixing in proportion, It is administered simultaneously or applies successively in proportion in proportion.
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Families Citing this family (7)

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EP3427682B1 (en) 2013-03-15 2020-09-23 Gyrus ACMI, Inc. (D.B.A. Olympus Surgical Technologies America) Combination electrosurgical device
CN107027766A (en) * 2017-04-28 2017-08-11 广西南宁益土生物科技有限责任公司 A kind of Synergistic bactericidal composition for preventing and treating sclerotinia sclerotiorum
CN107593719A (en) * 2017-10-17 2018-01-19 赖丰光 Argentiferous fruit and the composition pesticide of epoxiconazole
CN107915560A (en) * 2017-12-15 2018-04-17 安徽省农业科学院作物研究所 A kind of special fertilizer of the wheat increase yield suitable for Shajiang black soil
CN108207966A (en) * 2018-02-02 2018-06-29 深圳诺普信农化股份有限公司 Bactericidal composition
CN108378069A (en) * 2018-05-09 2018-08-10 惠州市梦芭迪工业产品设计中心(普通合伙) Bactericidal composition
CN110651793A (en) * 2019-10-23 2020-01-07 六夫丁作物保护有限公司 Pesticide sterilization composition and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101142190A (en) * 2005-03-16 2008-03-12 巴斯福股份公司 Nicotinanilides method for production thereof and agents comprising the same for prevention of fungal pests
CN102948424A (en) * 2011-08-28 2013-03-06 深圳诺普信农化股份有限公司 Boscalid-containing bactericidal composition
CN103947658A (en) * 2014-05-20 2014-07-30 深圳诺普信农化股份有限公司 Thifluzamide-containing bactericidal composition

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3963569B2 (en) * 1998-04-24 2007-08-22 三井化学株式会社 Plant disease control composition
EP1563733A1 (en) * 2004-02-12 2005-08-17 Bayer CropScience S.A. Fungicidal composition comprising a pyridylethylbenzamide derivative and a compound capable of inhibiting mitosis and cell division
EP1571143A1 (en) * 2004-02-12 2005-09-07 Bayer CropScience S.A. Fungicidal composition comprising a pyridylethylbenzamide derivative and a compound capable of inhibiting the transport of electrons of the respiratory chain in phytopathogenic fungal organisms
DE102005015677A1 (en) * 2005-04-06 2006-10-12 Bayer Cropscience Ag Synergistic fungicidal drug combinations
AU2010207788B2 (en) * 2009-01-30 2015-08-20 Bayer Cropscience Aktiengesellschaft Use of succinate dehydrogenase inhibitors for controlling powdery mildew primary infections
CA2806419C (en) * 2010-07-26 2018-08-21 Lorianne Fought Use of succinate dehydrogenase inhibitors and/or respiratory chain complex iii inhibitors for improving the ratio of harmful to beneficial microorganisms
CN107396929A (en) * 2010-12-01 2017-11-28 拜耳知识产权有限责任公司 Fluopyram is used to prevent and treat the nematode in crop and improves the purposes of yield
CN103155936B (en) * 2011-12-18 2014-08-20 深圳诺普信农化股份有限公司 Plant disease control composition containing phenazino-1-carboxylic acid

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101142190A (en) * 2005-03-16 2008-03-12 巴斯福股份公司 Nicotinanilides method for production thereof and agents comprising the same for prevention of fungal pests
CN102948424A (en) * 2011-08-28 2013-03-06 深圳诺普信农化股份有限公司 Boscalid-containing bactericidal composition
CN103947658A (en) * 2014-05-20 2014-07-30 深圳诺普信农化股份有限公司 Thifluzamide-containing bactericidal composition

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