CN106234408A - Bactericidal composition and application thereof - Google Patents

Bactericidal composition and application thereof Download PDF

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Publication number
CN106234408A
CN106234408A CN201610630815.7A CN201610630815A CN106234408A CN 106234408 A CN106234408 A CN 106234408A CN 201610630815 A CN201610630815 A CN 201610630815A CN 106234408 A CN106234408 A CN 106234408A
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shenqinmycin
component
sodium
bactericidal composition
preparation
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CN106234408B (en
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古越
李翔
陈熙
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Sichuan Lier Crop Science Co Ltd
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Sichuan Lier Crop Science Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of bactericidal composition and application thereof, relate to technical field of pesticide combination.The effective ingredient of bactericidal composition of the present invention contains shenqinmycin A and remaining antibacterial B, and wherein remaining antibacterial B is selected from the cyazofamid of sulphaguanidine azoles fungicide, the mould spirit of azoles fungicide, the CGA-173506 of pyroles antibacterial or the phosethyl-Al of organophosphorus fungicide.A Yu B weight ratio is 1:100～100:1.Effective constituents A is compounding with B has obvious potentiation, can effectively reduce the amount of application of each single dose.To expanding fungicidal spectrum and delaying the Genes For Plant Tolerance property of medicine to have preferably effect, moreover it is possible to extend the lasting period, there is obvious promotional value.Said composition can be added the auxiliary element allowing to use in other pesticide and is processed into arbitrary dosage form.This bactericidal composition can effectively prevent and treat banded sclerotial blight, rice blast, false smut, rust, powdery mildew, root rot, sclerotiniose, smut, droop etc..

Description

Bactericidal composition and application thereof
Technical field
The present invention relates to a kind of bactericidal composition and application thereof, particularly relate to a kind of containing effective ingredient shenqinmycin and work The bactericidal composition of property antibacterial and application thereof, belong to technical field of pesticide combination.
Background technology
In agricultural production, crops can healthy growth, in addition to being affected by worm, crop smothering, the preventing and treating to disease is the heaviest Want.Along with reinforcement and the sustainable development implementation of environmental protection ideas, efficient, low toxicity, high activity, low-residual have become pesticide and have sent out The inexorable trend of exhibition.But, even if efficient germicide, it is used alone for a long time, the most easily makes disease develop immunity to drugs, cause using The problems such as dose increasing, preventive effect reduction, lasting period shortening, are unfavorable for environment sustainable development.Therefore, how Scientific Usage of Drugs, fall The consumption of low chemical pesticide, improves drug effect, becomes the problem that plant protection art is badly in need of solving.
Cyazofamid (CyazofaMid) goes out also known as match seat, 4-chloro-2-cyano group-N, N-dimethyl-5-p-methylphenyl imidazoles-1- Sulfonamide, cyanogen azoles flusulfamide, section are good, belong to sulphaguanidine azoles fungicide.Cyazofamid is mitochondrial respiratory inhibitor, to oomycetes institute Having growth stage all to have effect, with other antibacterial without cross tolerance, have certain interior suction and therapeutic activity, the lasting period is long, resistance to Rain drop erosion, to crop, the mankind, Environmental security, but long-term single use, can make pathogenic bacteria that it is developed immunity to drugs unavoidably.
Mould spirit (hymexazol), also known as hydroxyisoxazole, hymexazol, molecular formula is C4H5NO2, chemical name: 3-hydroxyl- 5-methyl isoxazole.Mould spirit is systemic fungicide, is again a kind of soil sterilants simultaneously.This medicine and the ferrum in soil, aluminum Ions binding, the germination of suppression spore.Hymexazol by the root absorption of plant and can move in root system, produces at plant intracellular metabolite Two kinds of glucosides, are improved the effect of physiologically active to crop, it is thus possible to promote plant strain growth, point evil of root, the increase of root hair and The activity raising of root.Mould spirit is that the plant disease that multiple pathogenic fungi causes is had higher preventing and treating to tie by broad-spectrum germicide Really, but long-term single use, can make pathogenic bacteria that it is developed immunity to drugs unavoidably.
CGA-173506 (fludioxonil), also known as fluorine CGA-173506, coughs up bacterium ester, 4-(2,2-bis-fluoro-1,3-benzo dioxy-4- Base) pyrroles's-3-nitrile, the bacterium that do not falls dislike, suitable happy time, CGA-173506 is new pyrrole class non-internal-absorting wide-spectrum bactericide, its mechanism of action It is the transferring enzyme relevant by suppression thalline glucose phosphorylated, the growth of fungal mycelium can be suppressed, cause pathogenic bacteria dead.Right The microbial diseases of cause of disease such as Alternaria, Ascochyta, aspergillus, Fusarium, Helminthosporium, Rhizoctonia and Penicillium There is well protection and therapeutical effect, be used for preventing and treating snow rotten Fusariumsp, Semen Tritici aestivi black rot, rhizoctonia solani etc..
Phosethyl-Al (phosethyl-Al), Methylpartricin Sodium Laurylsulfate clean also known as Yimeiling, the spirit of epidemic disease frost, AL-PHOSETHYL aluminum tris(ethyl phosphite), mould epidemic disease, mycete Spirit, three second aluminum phosphate, fosetyl aluminium, be a kind of organic phosphates efficiently, wide spectrum, interior absorption low toxicity antibacterial, there is treatment and protection Effect.It can be with upper and lower Bidirectional Conduction in plant.This aqueous solubility is good, and interior suction permeability is strong, and the lasting period is longer, uses Safety.
Shenqinmycin, its structural formula is as shown in (I), and shenqinmycin is to be secreted through biological culture by pseudomonas fluorescens M18 A kind of antibiotics, there is wide spectrum Suppressing phytopathogens simultaneously and promote the antibacterial of dual-use function of Effects on Plant Growth, There is wide spectrum, efficient feature.
Containing frequently using of single-activity component antibacterial, causing most germs to create Drug resistance, preventive effect is not Preferable.Therefore, it is badly in need of a kind of novel product and substitutes the sterilised products of single-activity component, increase drug effect and delay the Drug resistance of disease Risk, reduces economic loss.
Summary of the invention
It is reasonable that the technical problem to be solved in the present invention is to provide a kind of component, and potentiation is notable, and bactericidal effect is good, medication Low cost, is not likely to produce Drug resistance, to the bactericidal composition of crop safety and application thereof.
For solving above-mentioned technical problem, the present invention provides technical scheme as follows:
The bactericidal composition of the present invention, its effective ingredient is shenqinmycin A and active bactericide B, shenqinmycin A and activity The weight ratio of antibacterial B be 1:100～100:1, preferably weight ratio be 1:50～50:1, more preferably weight ratio be 1:25～ 25:1.
In the bactericidal composition of the present invention, described active bactericide can be: the cyazofamid of sulphaguanidine azoles fungicide, Any one in the phosethyl-Al of the mould spirit of azoles fungicide, the CGA-173506 of pyroles antibacterial or organophosphorus fungicide etc. Kind.
Bactericidal composition of the present invention can also contain pesticidal preparations auxiliary element, be suitable for agriculturally using to make Dosage form.Described pesticidal preparations auxiliary element is the one in solvent, emulsifying agent, dispersant, stabilizer, antifreezing agent or wetting agent Or it is several.
The bactericidal composition of the present invention can be prepared as being suitable for any one dosage form of agricultural use by known method, than Preferably dosage form is dry suspending agent (DF), wettable powder (WP), water dispersible granules (WDG), suspending agent (SC), seed treatment Or suspension seed-coating agent etc..
A wettable powder (WP), is can homodisperse solid powder preparation in water.Wettable powder of the present invention except Add as outside the diluent such as white carbon, precipitated calcium carbonate, potter's clay, kieselguhr, attapulgite or inert substance in former medicine, also have from Subtype or nonionic surfactant (wetting agent, dispersant), wetting agent such as, alkylbenzenesulfonate (DBS-Na), alkyl Naphthalene sulfonate (pulls open powder), alkylphenol-polyethenoxy base ether sulfate, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulfate, Aliphatic alcohol sulfate, fatty alcohol ethylene oxide adduct sulfonate, alkyl amide taurate, aliphatic alcohol polyethenoxy base ether (JFC), content 0.1～30%；Dispersant such as naphthalene sulfonate, naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), wood Quality and derivant sulfonate (M-9, POLYFON etc.) thereof, polyoxyethylene poly-oxygen propylene aether block copolymer, alkyl phenol polyoxy second Alkene ether formaldehyde condensation products sulfate (SOPA), PVA, CMC content 0.1～30%；Possibly together with penetrating agent JFC, azone, thiophene ketone, One or more materials among penetrant t, content 0.3～30%；Stabilizer, foam inhibitor, anticaking agent etc. can also be contained Other auxiliary agent.
B water dispersible granules (WDG), is can very fast disintegrate in water, the granule of dispersion formation higher suspension dispersion.By Active component, the key element such as auxiliary agent and carrier such as wetting agent, dispersant, interleaving agent, disintegrating agent, stabilizer, adhesive composition.It helps Agent and carrier, and the compound method of precursor (preformed objects before pelletize) is analogous to wettable powder and suspending agent substantially.
C suspending agent (SC), is not water soluble solid pesticide or immiscible liquids pesticide dispersion in water or oil.By activity Composition, auxiliary agent (wetting dispersing agent, thickening agent, stabilizer, pH adjusting agent, defoamer, antifreezing agent) prepare through soybean dietary fiber. Wetting dispersing agent can be naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), lignin and derivant sulfonate thereof (M-9, POLYFON etc.), alkylphenol-polyethenoxy base ether sulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, alkyl Phenol polyethenoxy base phosphate ester, carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP), Polyethylene Glycol (PEG), condensation phosphorus One or more in hydrochlorate etc., content 0.1～12%；Thickening agent and stabilizer can be Xanthan gum (XG), polyvinyl alcohol (PVA) one or more, in Magnesiumaluminumsilicate etc., content 0.05～0.5%；PH adjusting agent and defoamer can add the most in right amount Add.
D dry suspending agent (Dry Flowable is called for short DF), is can very fast disintegrate in water, dispersion formation higher suspension dispersion The granule of system.Preparation method: the preparation obtained by the dehydration of suspending agent.Its auxiliary agent and carrier, and precursor (dehydrate Before preformed objects) compound method consistent with suspending agent.
Bactericidal composition of the present invention is applicable to prepare the pesticidal preparations of controlling plant diseases.It is particularly useful for making Prevent and treat rice sheath blight disease, rice blast, false smut, wheat rust, powdery mildew, root rotof flax, wheat sharp eyespot, rice banded sclerotial blight The pesticidal preparations of disease, root rot, sclerotinia rot of colza, maize head smut, capsicum wilt, cucurbits fusarium wilt etc..
Bactericidal composition of the present invention can provide with finished product preparation form, and i.e. in bactericidal composition, each material has mixed Close, or the composition of bactericidal composition can also provide with unit dose form, directly directly mix in bucket or tank, so before using After be diluted to required concentration.
Bactericidal composition of the present invention is applied to the method for controlling disease, and active component A and component B are with 1:100- The weight ratio of 100:1 is used, and further dispenser is than for 1:50-50:1, and further dispenser is than for 1:50-50:1, and it can be pressed Use after aforementioned proportion mixing, use the most simultaneously or use the most successively.Concrete method of application can be powder injection process, spray Mist method, smog method, fumigation and steaming method and seed treatment and soil treatment, ultra-low volume spray, mix fertilizer, spread fertilizer over the fields, pouring root.
Compared with prior art, the beneficial effects of the present invention is:
1, compared with single dose, compounding have obvious synergistic function, improves prevention effect；
2, compositions is made up of the effective ingredient that two kinds of mechanism of action are different, and bactericidal effect is good, is conducive to overcoming and delaying The drug-fast generation of pathogenic bacteria；
3, medicament mixture decreases dosage, thus reduces cost and alleviate the pollution to environment.
Instant component is reasonable, and treatment adds protective effect, and drug cost is low, and its activity and bactericidal effect are not each components The simple superposition of activity, but have significant potentiation, good to crop safety, meet the security requirement of pesticidal preparations.
Detailed description of the invention
For making the technical problem to be solved in the present invention, technical scheme and advantage clearer, specific embodiment will be used below It is described in detail, but the present invention is limited to absolutely not these examples.The following stated is only the preferable embodiment of the present invention, only in order to Explain the present invention, therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that, it is all the present invention's Any amendment, equivalent and the improvement etc. made within spirit and principle, should be included within the scope of the present invention. Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
The combination of active principles of different pesticide is made pesticide, is to develop and develop novel pesticide at present and prevent and treat agriculturally A kind of effective and quick way of resistance pathogenic bacteria.After the pestsides synthesis of different cultivars, typically exhibit a large amount of three kinds of effect classes Type: summation action, potentiation and antagonism.The good formula of compound synergic, it is possible to significantly improve actual prevention effect, fall The usage amount of low pesticide, thus contribute to delaying pathogenic bacteria drug-fast generation speed, it is the important means of integrated control disease.
The present composition is with shenqinmycin (A) for a kind of effective ingredient, and active bactericide (B) is another effective ingredient, Combine between them and plant disease is had obvious synergistic function, and be not only the simple folded of two kinds of pharmacy effects Adding, this can be clearly understood that from indoor virulence test result.
In order to prevent and treat the tomato late blight in agricultural production, inventor is with shenqinmycin and the cyanogen of sulphaguanidine azoles fungicide Frost azoles carries out the potentiation research compounded, method particularly includes:
Test uses tomato late blight for test object.
Former medicine is configured to the test medicine needed, and test method (blade method) is with reference to " People's Republic of China's agricultural row Industry standard ".Experiment is cultivated with pathogen in suitable culture medium, after Sporangium to be generated, with spore under aseptic washing, uses Double gauze filters, and makes sporangia suspension, is placed under 4 DEG C of low temperature, dark processing 0.5h-3h, tries it and discharge travelling spore Son, and regulate spore concentration to 1*105Individual spore/L, standby；Or directly prepare 1105Individual Sporangium/ml, suspension is as inoculation Body.Former medicine is configured to the test medicine needed, medicament is uniformly sprayed in vacuum side of blade, after medicinal liquid natural air drying, will be each Process vacuum side of blade upward, be emitted in moisture preservation box by after processing labelling.Often process no less than 10 leaves, repeat, and set only for 4 times Containing solvent and surfactant, blank is done in the process without effective ingredient.Spray with Sporangium or zoospore suspension Inoculation.After protectiveness test generally chemicals treatment, about 24h inoculates；Curative tests typically 24h before chemicals treatment Inoculation.Replace at continuous illumination every day/dark each 12H after inoculation, temperature is 18 DEG C.And after inoculation, in 24H, keep blade face to have Moisture film, later relative humidity is to cultivate 7 days under conditions of more than 90%.When blank disease leaf rate reaches more than 50%, point Incidence is managed in level investigation everywhere.Stage division is: 0 grade: do not fall ill；1 grade: only having a small amount of little scab on blade, scab accounts for leaf Area less than 10%；3 grades: on blade, scab accounts for leaf area 10%-25%；5 grades: on blade, scab accounts for leaf area 25%-50%； 7 grades: on blade, scab accounts for leaf area more than 50%；9 grades: full leaf morbidity is withered.
According to blank incidence, investigate each process disease index, calculate preventive effect.By the probit value of suppression ratio and Linear regression analysis between the logarithm value of series concentration, obtains the EC50 value of each medicament, calculates mixture with co-toxicity coefficient table Co-toxicity coefficient (CTC), evaluate the reagent agent activity to pathogenic bacteria with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as potentiation；CTC≤80 show as antagonism；80 CTC 120 shows as summation action.
Table 1 shenqinmycin and the compounding Toxicity Determination to tomato late blight of cyazofamid
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shenqinmycin (A) —— Y=1.6974X+3.8406 4.8198 ——
Cyazofamid (B1) —— Y=1.1207X+3.0833 51.3205 ——
A:B1 1:100 Y=0.8133X+3.7145 38.0600 123.08
A:B1 1:50 Y=0.8392X+3.7158 33.9062 127.29
A:B1 1:25 Y=0.8094X+3.8296 27.9272 134.08
A:B1 1:1 Y=0.7258X+4.4771 5.2537 167.72
A:B1 25:1 Y=0.6666X+4.6275 3.6275 137.67
A:B1 50:1 Y=0.7039X+4.6006 3.6931 132.88
A:B1 100:1 Y=0.7284X+4.5749 3.83361 126.87
Toxicity Determination result table 1 shows, in weight ratio 1:100-100:1, in the range of particularly 1:50-50:1, Especially in the range of weight ratio 1:25-25:1, shenqinmycin is compounding with cyazofamid has obvious potentiation work to plant disease With, compounding synergistic effect becomes apparent from, and prevention effect is excellent and is better than single dose kind.
In order to prevent and treat the sclerotinia rot of colza in agricultural production, inventor is with shenqinmycin and the mould spirit of azoles fungicide Carry out the potentiation research compounded, method particularly includes:
Test uses sclerotinia rot of colza for test object.
Former medicine is configured to the test medicine needed, with reference to mycelial growth rate method, the preparation of strain: the most sterilized Culture medium is poured into about 10mLPDA culture medium.Aseptically, from the strain inclined plane of Sclerotinia sclerotiorum, a mycelia is taken Block, is placed in the middle of culture dish.Cultivate several generations continuously, the most before the test 3d, in inoculation once, train under the conditions of 24～26 DEG C Support, standby.The making of toxic flat board, after first the PDA culture medium prepared being dissolved, places cooling.Aseptically, will Medicinal liquid 1mL adds in sterilized culture dish, then adds in the culture dish of existing medicinal liquid by pipette, extract 9mL culture medium, Rocking gently shakes up (left 3 circles, right 3 circles), makes the uniform flat board of thin and thick.Connect bacterium: punched by off-the-shelf pathogen Bacterium cake made by device, then moves on the pastille flat board solidified with Inoculating needle picking bacterium cake, and mycelia one faces down.Labelling, puts 24 ～26 DEG C of incubators are cultivated.Treat matched group colony diameter length to about 6cm, decussation method investigate other process data also Record.
According to blank incidence, record lesion diameter, calculate each percentage rate processing suppression scab growth, pass through Linear regression analysis between probit value and the logarithm value of series concentration of suppression ratio, obtains the EC50 value of each medicament, with poison altogether Coefficient table calculates the co-toxicity coefficient (CTC) of mixture, evaluates the reagent agent activity to pathogenic bacteria with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as potentiation；CTC≤80 show as antagonism；80 CTC 120 shows as summation action.
Table 2 shenqinmycin and the compounding Toxicity Determination to sclerotinia rot of colza of mould spirit
Toxicity Determination result table 2 shows, in weight ratio 1:100-100:1, in the range of particularly 1:50-50:1, Especially in the range of weight ratio 1:25-25:1, shenqinmycin is compounding with mould spirit has obvious potentiation work to plant disease With, prevention effect is excellent and is better than single dose kind.
In order to prevent and treat the wheat sharp eyespot in agricultural production, inventor is with shenqinmycin and the CGA-173506 of pyroles antibacterial Carry out the potentiation research compounded, method particularly includes:
Test uses wheat sharp eyespot for test object.
Former medicine is configured to need test medicine, test method: fungicide bioassay pattern Broad Bean Leaves method, With reference to " People's Republic of China's agricultural industry criteria ".Choose Semen Viciae fabae susceptible variety potted plant, clip same section, growing way is consistent, Carrying petiolate blade, put in culture dish, moisturizing is standby, experimental pharmacy uses former medicinal water dissolution dilute, arranges 5 quality Concentration, fully infiltrates 5S by blade in pre-configured good medicinal liquid, drains unnecessary medicinal liquid, after natural air drying, after processing labelling Moisturizing is cultivated, and test sets process without medicament as blank.The one side of mycelia there is is to connect a diameter of 5mm bacterium cake with inoculator Plant in processing central vane, each process 30 leaves of inoculation.Protectiveness test 24h inoculation, curative tests after chemicals treatment 24h inoculation before chemicals treatment.In inoculation is placed on that growth cabinet or constant temperature are real and has illumination humidistat, Celsius in temperature 26 Spend-28 degrees Celsius, cultivate under conditions of relative humidity 80%-90%.According to blank incidence, use kind of calliper record Each vaccination scab length and width, represents lesion diameter record lesion diameter (unit mm) with length and width meansigma methods. according to tune Look into data, by preventing and treating result publicity: preventive effect=(blank diameter-process diameter)/blank diameter * 100, calculate each process and suppress disease The percentage rate of speckle growth, by the linear regression analysis between probit value and the logarithm value of series concentration of suppression ratio, obtains each The EC50 value of medicament, calculates the co-toxicity coefficient (CTC) of mixture, evaluates reagent agent to pathogenic bacteria with this with co-toxicity coefficient table Activity.
According to blank incidence, record lesion diameter, calculate each percentage rate processing suppression scab growth, pass through Linear regression analysis between probit value and the logarithm value of series concentration of suppression ratio, obtains the EC50 value of each medicament, with poison altogether Coefficient table calculates the co-toxicity coefficient (CTC) of mixture, evaluates the reagent agent activity to pathogenic bacteria with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as potentiation；CTC≤80 show as antagonism；80 CTC 120 shows as summation action.
Table 3 shenqinmycin and the compounding Toxicity Determination to wheat sharp eyespot of CGA-173506
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shenqinmycin (A) —— Y=1.9338X+2.0570 33.2586 ——
CGA-173506 (B3) —— Y=1.9057X+2.2920 26.3637 ——
A:B3 1:100 Y=1.2830X+3.2849 21.5031 121.62
A:B3 1:50 Y=1.0906X+3.6234 18.2905 144.72
A:B3 1:25 Y=0.9393X+3.8792 15.6047 170.30
A:B3 1:1 Y=0.9717X+3.8323 15.6883 187.48
A:B3 25:1 Y=1.4046X+3.2208 18.4823 178.15
A:B3 50:1 Y=15701X+2.8471 23.5048 140.77
A:B3 100:1 Y=17365X+2.5490 25.7910 128.61
Toxicity Determination result table 3 shows, in weight ratio 1:100-100:1, in the range of particularly 1:50-50:1, Especially in the range of weight ratio 1:25-25:1, shenqinmycin is compounding with CGA-173506 has obvious potentiation work to plant disease With, prevention effect is excellent and is better than single dose kind.
In order to prevent and treat the downy mildew of lettuce in agricultural production, inventor is with shenqinmycin and the triethylphosphine of organophosphorus fungicide Acid aluminum carries out the potentiation research compounded, method particularly includes:
Test uses downy mildew of lettuce for test object.
Former medicine is configured to the test medicine needed, and test method (plate blade method) is with reference to " People's Republic of China's agriculture Industry industry standard ".Choosing Caulis et Folium Lactucae sativae susceptible variety potted plant, clip same section, growing way is consistent, carries petiolate blade, puts cultivation In ware, moisturizing is standby.Single dose and each Mixed Pharmacy are arranged 5 variable concentrations gradients (on the basis of pilot study result, root Different with proportioning according to different agents combination, concentration arranges the most different, and bacteriostasis rate sets in the range of 5%～95%).Will Medicinal liquid uniformly sprays in vacuum side of blade, after medicinal liquid natural air drying, each process blade and blade is gone up dorsad, discharges by after processing labelling In moisture preservation box standby, test sets process without medicament as blank.Inoculate with ready Fresh spores drop 10uL In vacuum side of blade, every blade inoculation 4, often process no less than 5 leaves, protectiveness test is inoculated for 24 hours after chemicals treatment, Curative tests, in chemicals treatment inoculation in first 24 hours, then covers ware lid, and being placed in temperature is 17～22 DEG C of relative humiditys 90% Cultivating 5 7d in above constant temperature and humidity incubator, continuous illumination every day/night 12h is alternately；According to blank morbidity feelings Condition, records lesion diameter, calculates each percentage rate processing suppression scab growth, by the probit value of suppression ratio and series concentration Linear regression analysis between logarithm value, obtains the EC50 value of each medicament, calculates the co-toxicity coefficient of mixture with co-toxicity coefficient table (CTC), the reagent agent activity to pathogenic bacteria is evaluated with this.
According to blank incidence, record lesion diameter, calculate each percentage rate processing suppression scab growth, pass through Linear regression analysis between probit value and the logarithm value of series concentration of suppression ratio, obtains the EC50 value of each medicament, with poison altogether Coefficient table calculates the co-toxicity coefficient (CTC) of mixture, evaluates the reagent agent activity to pathogenic bacteria with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as potentiation；CTC≤80 show as antagonism；80 CTC 120 shows as summation action.
Table 4 shenqinmycin and the compounding Toxicity Determination to downy mildew of lettuce of phosethyl-Al
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shenqinmycin (A) —— Y=1.8664X+1.9415 43.5238 ——
Phosethyl-Al (B4) —— Y=1.8435X+1.2674 105.8708 ——
A:B4 1:100 Y=1.7797X+1.7255 69.1631 150.93
A:B4 1:50 Y=1.5902X+2.2177 56.1834 183.29
A:B4 1:25 Y=1.2482X+2.8734 50.5453 198.54
A:B4 1:1 Y=1.3733X+3.1105 23.7598 259.62
A:B4 25:1 Y=1.3500X+3.0151 29.5324 150.79
A:B4 50:1 Y=1.0815X+3.3718 32.0268 137.49
A:B4 100:1 Y=0.8909X+3.6372 33.8661 129.27
Toxicity Determination result table 4 shows, in weight ratio 1:100-100:1, in the range of particularly 1:50-50:1, Especially in the range of weight ratio 1:25-25:1, shenqinmycin is compounding with phosethyl-Al has obvious potentiation to plant disease Effect, prevention effect is excellent and is better than single dose kind.
Toxicity Determination table 1-table 4 result shows, in the scope of weight ratio 1:100-100:1, particularly 1:50-50:1 In, shenqinmycin is multiple with phosethyl-Al with CGA-173506, shenqinmycin with mould spirit, shenqinmycin with cyazofamid, shenqinmycin Pairing plant disease has obvious potentiation, and prevention effect is excellent and is better than single dose kind.Especially in weight ratio 1:25- In the range of 25:1, shenqinmycin and cyazofamid, shenqinmycin and mould spirit shenqinmycin and CGA-173506, shenqinmycin and three second The synergistic effect that aluminum phosphate compounds becomes apparent from.
For making the technical problem to be solved in the present invention, technical scheme and advantage clearer, in weight ratio 1:100～100: In the range of 1, build the embodiment that shenqinmycin is compounding with cyazofamid, mould spirit, CGA-173506 or phosethyl-Al respectively, carry out in detail Thin description, but the present invention is limited to absolutely not these examples.
It addition, inventor also by experiment find, shenqinmycin respectively with cyazofamid, mould spirit, CGA-173506 or triethylphosphine The compounding effect of acid aluminum, is better than the compounding of other antibiotics antibacterial and cyazofamid, mould spirit, CGA-173506 or phosethyl-Al Effect.In order to clearly demonstrate this problem, in the range of weight ratio 1:100～100:1, build other antibiotics antibacterial The comparative example compounding with cyazofamid, mould spirit, CGA-173506 or phosethyl-Al, is described in detail.But owing to length is limited, only Representative several comparative examples can be enumerated.It should be pointed out that, be not limited in limited comparative example.
The bactericidal composition of the present invention can be prepared as being suitable for any one dosage form of agricultural use by known method, than Preferably dosage form is wettable powder, aqueous suspension agent, oil-suspending agent, suspended emulsion, water dispersible granules.Percentage ratio in all formula All it is weight percentage.The processing technique of the various dosage form of the present composition is prior art, can have according to different situations Changed.
Below in conjunction with embodiment, present invention is described further.
One, shenqinmycin compounds with cyazofamid
Embodiment 1:20.2% shenqinmycin cyazofamid suspending agent (1:100)
Component: shenqinmycin 0.2%, cyazofamid 20%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinyl Ether 4%, 1,2-the third two Alcohol 6%, XG0.32%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method: each component mixed, through high speed shear mix homogeneously, then passes through sand mill sand milling 2-3 hour, makes to put down All particle diameters reach 1-5 micron, can be prepared by 20.2% shenqinmycin cyazofamid suspending agent.
Comparative example 1:20.2% polyoxin cyazofamid suspending agent (1:100)
Component: polyoxin 0.2%, cyazofamid 20%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinyl Ether 4%, 1,2-the third two Alcohol 6%, XG0.32%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.Preparation method: ibid.
Embodiment 2:16.8% shenqinmycin cyazofamid water dispersible granules (1:55)
Component: shenqinmycin 0.3%, cyazofamid 16.5%, lignin sodium naphthalene sulfonate 6%, polycarboxylic acids sodium 3%, anhydrous sulfur Acid sodium 10%, Kaolin complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 16.8% shenqinmycin cyazofamid water dispersible granules.
Embodiment 3:25.5% shenqinmycin cyazofamid wettable powder (1:50)
Component: shenqinmycin 0.5%, cyazofamid 25%, sodium lauryl sulphate 4%, sodium lignin sulfonate 6%, Linesless charcoal Black 6%, precipitated calcium carbonate complements to 100%.
Preparation method: by each component mix homogeneously, first pass through mechanical activation comminution, then after comminution by gas stream, mix homogeneously, i.e. 25.5% shenqinmycin cyazofamid wettable powder can be prepared.
Comparative example 2:25.5% kasugarnycin cyazofamid wettable powder (1:50)
Component: kasugarnycin 0.5%, cyazofamid 25%, sodium lauryl sulphate 4%, sodium lignin sulfonate 6%, Linesless charcoal Black 6%, precipitated calcium carbonate complements to 100%.Preparation method: ibid.
Embodiment 4:35% shenqinmycin cyazofamid dry suspending agent (1:34)
Component: shenqinmycin 1%, cyazofamid 34%, lignin sodium naphthalene sulfonate 10%, sodium lignin sulfonate 8%, white carbon 10%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, Kaolin complements to 100%.Soft water 150-200%.
Preparation method: add the water of 1.5 2.0 times of preparation amount of preparation, each component is mixed, through high speed shear mixing all Even, then pass through sand mill sand milling 2-3 hour, make mean diameter reach 1-5 micron, it is mould that dehydrate can be prepared by 35% Shen piperazine Element cyazofamid dry suspending agent.
Embodiment 5:26% shenqinmycin cyazofamid water dispersible granules (1:25)
Component: shenqinmycin 1%, cyazofamid 25%, sodium naphthalene sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8% is sliding Stone powder complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 26% shenqinmycin cyazofamid water dispersible granules.
Comparative example 3:26% Ningnanmycin cyazofamid water dispersible granules (1:25)
Component: Ningnanmycin 1%, cyazofamid 25%, sodium naphthalene sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8% is sliding Stone powder complements to 100%.Preparation method: ibid.
Embodiment 6:32% shenqinmycin cyazofamid suspending agent (1:15)
Component: shenqinmycin 2%, cyazofamid 30%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 4%, fatty alcohol polyoxy second Alkene ether 3%, ethylene glycol 6%, XG0.25%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method: each component mixed, through high speed shear mix homogeneously, then passes through sand mill sand milling 2-3 hour, makes to put down All particle diameters reach 1-5 micron, can be prepared by 16% shenqinmycin cyazofamid suspending agent.
Embodiment 7:20% shenqinmycin cyazofamid wettable powder (1:1)
Component: shenqinmycin 10%, cyazofamid 10%, dodecylbenzene sodium sulfonate 4%, dodecyl naphthalene sulphonate formaldehyde Condensation substance 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.
Preparation method: by each component mix homogeneously, first pass through mechanical activation comminution, then after comminution by gas stream, mix homogeneously, i.e. 20% shenqinmycin cyazofamid wettable powder can be prepared.
Comparative example 4:20% polyoxin cyazofamid wettable powder (1:1)
Component: polyoxin 10%, cyazofamid 10%, dodecylbenzene sodium sulfonate 4%, dodecyl naphthalene sulphonate formaldehyde Condensation substance 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.Preparation method: ibid.
Comparative example 5:20% kasugarnycin cyazofamid wettable powder (1:1)
Component: kasugarnycin 10%, cyazofamid 10%, dodecylbenzene sodium sulfonate 4%, dodecyl naphthalene sulphonate formaldehyde Condensation substance 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.Preparation method: ibid.
Embodiment 8:21% shenqinmycin cyazofamid water dispersible granules (19:2)
Component: shenqinmycin 19%, cyazofamid 2%, sodium lauryl sulphate 3%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 6%, Pulvis Talci complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 21% shenqinmycin cyazofamid water dispersible granules.
Embodiment 9:16% shenqinmycin cyazofamid dry suspending agent (15:1)
Component: shenqinmycin 15%, cyazofamid 1%, lignin sodium naphthalene sulfonate 8%, sodium lignin sulfonate 8%, white carbon 6%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, Kaolin complements to 100%.Soft water 150-200%.
Preparation method: add the water of 1.5 2.0 times of preparation amount of preparation, each component is mixed, through high speed shear mixing all Even, then pass through sand mill sand milling 2-3 hour, make mean diameter reach 1-5 micron, it is mould that dehydrate can be prepared by 16% Shen piperazine Element cyazofamid dry suspending agent.
Embodiment 10:26% shenqinmycin cyazofamid water dispersible granules (25:1)
Component: shenqinmycin 25%, cyazofamid 1%, sodium lauryl sulphate 3%, lignin sodium naphthalene sulfonate 6%, anhydrous Sodium sulfate 8%, Kaolin complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 26% shenqinmycin cyazofamid water dispersible granules.
Comparative example 6:26% Ningnanmycin cyazofamid water dispersible granules (25:1)
Component: Ningnanmycin 25%, cyazofamid 1%, sodium lauryl sulphate 3%, lignin sodium naphthalene sulfonate 6%, anhydrous Sodium sulfate 8%, Kaolin complements to 100%.Preparation method: ibid.
Embodiment 11:23% shenqinmycin cyazofamid suspending agent (45:1)
Component: shenqinmycin 22.5%, cyazofamid 0.5%, polycarboxylic acids sodium 4%, fatty alcohol oxygen vinyl Ether 3%, ethylene glycol 5%, XG0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method: each component mixed, through high speed shear mix homogeneously, then passes through sand mill sand milling 2-3 hour, makes to put down All particle diameters reach 1-5 micron, can be prepared by 23% shenqinmycin cyazofamid suspending agent.
Embodiment 12:20.4% shenqinmycin cyazofamid suspending agent (50:1)
Component: shenqinmycin 20%, cyazofamid 0.4%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinyl Ether 3%, 1,2-the third two Alcohol 5%, XG0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method: each component mixed, through high speed shear mix homogeneously, then passes through sand mill sand milling 2-3 hour, makes to put down All particle diameters reach 1-5 micron, can be prepared by 20.4% shenqinmycin cyazofamid suspending agent.
Comparative example 7:20.4% kasugarnycin cyazofamid suspending agent (50:1)
Component: kasugarnycin 20%, cyazofamid 0.4%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinyl Ether 3%, 1,2-the third two Alcohol 5%, XG0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.Preparation method: ibid.
Embodiment 13:20.2% shenqinmycin cyazofamid wettable powder (100:1)
Component: shenqinmycin 20%, cyazofamid 0.2%, dodecylbenzene sodium sulfonate 3%, lignin sodium naphthalene sulfonate 6%, White carbon 6%, Pulvis Talci complements to 100%.
Preparation method: by each component mix homogeneously, first pass through mechanical activation comminution, then after comminution by gas stream, mix homogeneously, i.e. 20.2% shenqinmycin cyazofamid wettable powder can be prepared.
Comparative example 8:20.2% polyoxin cyazofamid wettable powder (100:1)
Component: polyoxin 20%, cyazofamid 0.2%, dodecylbenzene sodium sulfonate 3%, lignin sodium naphthalene sulfonate 6%, White carbon 6%, Pulvis Talci complements to 100%.Preparation method: ibid.
Two, shenqinmycin compounds with mould spirit
Embodiment 14:20.2% shenqinmycin mould Lingshui Spring dispersible granule (1:100)
Component: shenqinmycin 0.2%, mould clever 20%, sodium lauryl sulphate 3%, polycarboxylic acids sodium 4%, anhydrous slufuric acid Sodium 8%, Kaolin complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 20.2% shenqinmycin mould Lingshui Spring dispersible granule.
Comparative example 9:20.2% polyoxin mould Lingshui Spring dispersible granule (1:100)
Component: polyoxin 0.2%, mould clever 20%, sodium lauryl sulphate 3%, polycarboxylic acids sodium 4%, anhydrous slufuric acid Sodium 8%, Kaolin complements to 100%.Preparation method: ibid.
Embodiment 15:16.8% shenqinmycin mould spirit suspending agent (1:55)
Component: shenqinmycin 0.3%, mould clever 16.5%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 3%, poly alkyl alcohol Oxygen vinyl Ether 3%, ethylene glycol 6%, XG 0.32%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method: each component mixed, through high speed shear mix homogeneously, then passes through sand mill sand milling 2-3 hour, makes to put down All particle diameters reach 1-5 micron, can be prepared by 16.8% shenqinmycin mould spirit suspending agent.
Embodiment 16:25.5% shenqinmycin mould spirit wettable powder (1:50)
Component: shenqinmycin 0.5%, mould clever 25%, sodium naphthalene sulfonate 5%, sodium lauryl sulphate 3%, white carbon 10%, Pulvis Talci complements to 100%.
Preparation method: by each component mix homogeneously, first pass through mechanical activation comminution, then after comminution by gas stream, mix homogeneously, i.e. 25.5% shenqinmycin mould spirit wettable powder can be prepared.
Comparative example 10:25.5% tetramycin mould spirit wettable powder (1:50)
Component: tetramycin 0.5%, mould clever 25%, sodium naphthalene sulfonate 5%, sodium lauryl sulphate 3%, white carbon 10%, Pulvis Talci complements to 100%.Preparation method: ibid.
Embodiment 17:35% shenqinmycin mould spirit dry suspending agent (1:34)
Component: shenqinmycin 1%, mould clever 34%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 8%, organosilicon Defoamer 0.4%, sodium benzoate 0.1%, white carbon 10%, Kaolin complements to 100%.Soft water 150-200%.
Preparation method: add the water of 1.5 2.0 times of preparation amount of preparation, each component is mixed, through high speed shear mixing all Even, then pass through sand mill sand milling 2-3 hour, make mean diameter reach 1-5 micron, it is mould that dehydrate can be prepared by 35% Shen piperazine Element mould spirit dry suspending agent.
Embodiment 18:26% shenqinmycin mould Lingshui Spring dispersible granule (1:25)
Component: shenqinmycin 1%, mould clever 25%, dodecylbenzene sodium sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous slufuric acid Sodium 8%, Pulvis Talci complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 26% shenqinmycin mould Lingshui Spring dispersible granule.
Comparative example 11:26% polyoxin mould Lingshui Spring dispersible granule (1:25)
Component: polyoxin 1%, mould clever 25%, dodecylbenzene sodium sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous slufuric acid Sodium 8%, Pulvis Talci complements to 100%.Preparation method: ibid.
Embodiment 19:32% shenqinmycin mould spirit suspending agent (1:15)
Component: shenqinmycin 2%, mould clever 30%, polycarboxylic acids sodium 4%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG 0.26%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method: each component mixed, through high speed shear mix homogeneously, then passes through sand mill sand milling 2-3 hour, makes to put down All particle diameters reach 1-5 micron, can be prepared by 32% shenqinmycin mould spirit suspending agent.
Embodiment 20:20% shenqinmycin mould spirit wettable powder (1:1)
Component: shenqinmycin 10%, mould clever 10%, lignin sodium naphthalene sulfonate 6%, dodecylbenzene sodium sulfonate 3%, in vain White carbon black 10%, Kaolin complements to 100%.
Preparation method: by each component mix homogeneously, first pass through mechanical activation comminution, then after comminution by gas stream, mix homogeneously, i.e. 20% shenqinmycin mould spirit wettable powder can be prepared.
Comparative example 12:20% polyoxin mould spirit wettable powder (1:1)
Component: polyoxin 10%, mould clever 10%, lignin sodium naphthalene sulfonate 6%, dodecylbenzene sodium sulfonate 3%, in vain White carbon black 10%, Kaolin complements to 100%.Preparation method: ibid.
Embodiment 21:21% shenqinmycin mould Lingshui Spring dispersible granule (19:2)
Component: shenqinmycin 19%, mould clever 2%, dodecyl sodium sulfate 4%, sodium lauryl sulphate 4%, anhydrous Sodium sulfate 10%, Pulvis Talci complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 21% shenqinmycin mould Lingshui Spring dispersible granule.
Embodiment 22:16% shenqinmycin mould spirit dry suspending agent (15:1)
Component: shenqinmycin 15%, mould clever 1%, polycarboxylic acids sodium 4%, lignin sodium naphthalene sulfonate 10%, organosilicon froth breaking Agent 0.4%, sodium benzoate 0.1%, white carbon 10%, Kaolin complements to 100%.Soft water 150-200%.
Preparation method: add the water of 1.5 2.0 times of preparation amount of preparation, each component is mixed, through high speed shear mixing all Even, then pass through sand mill sand milling 2-3 hour, make mean diameter reach 1-5 micron, it is mould that dehydrate can be prepared by 16% Shen piperazine Element mould spirit dry suspending agent.
Embodiment 23:26% shenqinmycin mould Lingshui Spring dispersible granule (25:1)
Component: shenqinmycin 25%, mould clever 1%, polycarboxylic acids sodium 4%, sodium lauryl sulphate 4%, anhydrous sodium sulfate 10%, Kaolin complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 26% shenqinmycin mould Lingshui Spring dispersible granule.
Comparative example 13:26% tetramycin mould Lingshui Spring dispersible granule (25:1)
Component: tetramycin 25%, mould clever 1%, polycarboxylic acids sodium 4%, sodium lauryl sulphate 4%, anhydrous sodium sulfate 10%, Kaolin complements to 100%.Preparation method: ibid.
Embodiment 24:23% shenqinmycin mould spirit suspending agent (45:1)
Component: shenqinmycin 22.5%, mould clever 0.5%, polycarboxylic acids sodium 3%, OPEO sodium sulfonate 3%, ethylene glycol 6%, XG 0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method: each component mixed, through high speed shear mix homogeneously, then passes through sand mill sand milling 2-3 hour, makes to put down All particle diameters reach 1-5 micron, can be prepared by 23% shenqinmycin mould spirit suspending agent.
Embodiment 25:20.4% shenqinmycin mould spirit dry suspending agent (50:1)
Component: shenqinmycin 20%, mould clever 0.4%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 10%, organic Silicon defoaming agent 0.4%, sodium benzoate 0.1%, white carbon 6%, Pulvis Talci complements to 100%.Soft water 150-200%.
Preparation method: add the water of 1.5 2.0 times of preparation amount of preparation, each component is mixed, through high speed shear mixing all Even, then pass through sand mill sand milling 2-3 hour, make mean diameter reach 1-5 micron, it is mould that dehydrate can be prepared by 20.4% Shen piperazine Element mould spirit dry suspending agent.
Comparative example 14:20.4% polyoxin mould spirit dry suspending agent (50:1)
Component: polyoxin 20%, mould clever 0.4%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 10%, organic Silicon defoaming agent 0.4%, sodium benzoate 0.1%, white carbon 6%, Pulvis Talci complements to 100%.Soft water 150-200%.Preparation side Method: ibid.
Embodiment 26:20.2% shenqinmycin mould spirit wettable powder (100:1)
Component: shenqinmycin 20%, mould clever 0.2%, dodecylbenzene sodium sulfonate 3%, lignin sodium naphthalene sulfonate 6%, White carbon 8%, Kaolin complements to 100%.
Preparation method: by each component mix homogeneously, first pass through mechanical activation comminution, then after comminution by gas stream, mix homogeneously, i.e. 20.2% shenqinmycin mould spirit wettable powder can be prepared.
Comparative example 15:20.2% tetramycin mould spirit wettable powder (100:1)
Component: tetramycin 20%, mould clever 0.2%, dodecylbenzene sodium sulfonate 3%, lignin sodium naphthalene sulfonate 6%, White carbon 8%, Kaolin complements to 100%.Preparation method: ibid.
Three, shenqinmycin compounds with CGA-173506
Embodiment 27:20.2% shenqinmycin CGA-173506 suspending agent (1:100)
Component: shenqinmycin 0.2%, CGA-173506 20%, polycarboxylic acids sodium 3%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG 0.32%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method: each component mixed, through high speed shear mix homogeneously, then passes through sand mill sand milling 2-3 hour, makes to put down All particle diameters reach 1-5 micron, can be prepared by 20.2% shenqinmycin CGA-173506 suspending agent.
Comparative example 16:20.2% polyoxin CGA-173506 suspending agent (1:100)
Component: polyoxin 0.2%, CGA-173506 20%, polycarboxylic acids sodium 3%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG 0.32%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.Preparation method: ibid.
Embodiment 28:16.8% shenqinmycin CGA-173506 water dispersible granules (1:55)
Component: shenqinmycin 0.3%, CGA-173506 16.5%, dodecylbenzene sodium sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous Sodium sulfate 8%, Pulvis Talci complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 16.8% shenqinmycin CGA-173506 water dispersible granules.
Comparative example 17:16.8% jingganmycin CGA-173506 water dispersible granules (1:55)
Component: jingganmycin 0.3%, CGA-173506 16.5%, dodecylbenzene sodium sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous Sodium sulfate 8%, Pulvis Talci complements to 100%.Preparation method: ibid.
Embodiment 29:25.5% shenqinmycin CGA-173506 wettable powder (1:50)
Component: shenqinmycin 0.5%, CGA-173506 25%, dodecylbenzene sodium sulfonate 4%, naphthalenesulfonic acid-formaldehyde condensate 8%, white carbon 6%, precipitated calcium carbonate complements to 100%.
Preparation method: by each component mix homogeneously, first pass through mechanical activation comminution, then after comminution by gas stream, mix homogeneously, i.e. 25.5% shenqinmycin CGA-173506 wettable powder can be prepared.
Comparative example 18:25.5% kasugarnycin CGA-173506 wettable powder (1:50)
Component: kasugarnycin 0.5%, CGA-173506 25%, dodecylbenzene sodium sulfonate 4%, naphthalenesulfonic acid-formaldehyde condensate 8%, white carbon 6%, precipitated calcium carbonate complements to 100%.Preparation method: ibid.
Embodiment 30:35% shenqinmycin CGA-173506 dry suspending agent (1:34)
Component: shenqinmycin 1%, CGA-173506 34%, lignin sodium naphthalene sulfonate 10%, polycarboxylic acids sodium 4%, white carbon 5%, Organic silicon defoamer 0.4%, sodium benzoate 0.1%, Pulvis Talci complements to 100%.Soft water 150-200%.
Preparation method: add the water of 1.5 2.0 times of preparation amount of preparation, each component is mixed, through high speed shear mixing all Even, then pass through sand mill sand milling 2-3 hour, make mean diameter reach 1-5 micron, it is mould that dehydrate can be prepared by 35% Shen piperazine Element CGA-173506 dry suspending agent.
Embodiment 31:26% shenqinmycin CGA-173506 water dispersible granules (1:25)
Component: shenqinmycin 1%, CGA-173506 25%, sodium naphthalene sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 10% is sliding Stone powder complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 26% shenqinmycin CGA-173506 water dispersible granules.
Comparative example 19:26% Ningnanmycin CGA-173506 water dispersible granules (1:25)
Component: Ningnanmycin 1%, CGA-173506 25%, sodium naphthalene sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 10% is sliding Stone powder complements to 100%.Preparation method: ibid.
Embodiment 32:32% shenqinmycin CGA-173506 suspending agent (1:15)
Component: shenqinmycin 2%, CGA-173506 30%, polycarboxylic acids sodium 3%, OPEO sodium sulfonate 3%, second Glycol 6%, XG 0.25%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method: each component mixed, through high speed shear mix homogeneously, then passes through sand mill sand milling 2-3 hour, makes to put down All particle diameters reach 1-5 micron, can be prepared by 32% shenqinmycin CGA-173506 suspending agent.
Comparative example 20:32% tetramycin CGA-173506 suspending agent (1:15)
Component: tetramycin 2%, CGA-173506 30%, polycarboxylic acids sodium 3%, OPEO sodium sulfonate 3%, second Glycol 6%, XG 0.25%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.Preparation method: with On.
Embodiment 33:20% shenqinmycin CGA-173506 wettable powder (1:1)
Component: shenqinmycin 10%, CGA-173506 10%, polycarboxylic acids sodium 5%, sodium lauryl sulphate 3%, white carbon 8%, Precipitated calcium carbonate complements to 100%.
Preparation method: by each component mix homogeneously, first pass through mechanical activation comminution, then after comminution by gas stream, mix homogeneously, i.e. 20% shenqinmycin CGA-173506 wettable powder can be prepared.
Comparative example 21:20% polyoxin CGA-173506 wettable powder (1:1)
Component: polyoxin 10%, CGA-173506 10%, polycarboxylic acids sodium 5%, sodium lauryl sulphate 3%, white carbon 8%, Precipitated calcium carbonate complements to 100%.Preparation method: ibid.
Comparative example 22:20% kasugarnycin CGA-173506 wettable powder (1:1)
Component: kasugarnycin 10%, CGA-173506 10%, polycarboxylic acids sodium 5%, sodium lauryl sulphate 3%, white carbon 8%, Precipitated calcium carbonate complements to 100%.Preparation method: ibid.
Embodiment 34:21% shenqinmycin CGA-173506 water dispersible granules (19:2)
Component: shenqinmycin 19%, CGA-173506 2%, dodecylbenzene sodium sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous slufuric acid Sodium 10%, Pulvis Talci complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 21% shenqinmycin CGA-173506 water dispersible granules.
Embodiment 35:16% shenqinmycin CGA-173506 dry suspending agent (15:1)
Component: shenqinmycin 15%, CGA-173506 1%, lignin sodium naphthalene sulfonate 8%, sodium lignin sulfonate 8%, white carbon 6%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, Kaolin complements to 100%.Soft water 150-200%.
Preparation method: add the water of 1.5 2.0 times of preparation amount of preparation, each component is mixed, through high speed shear mixing all Even, then pass through sand mill sand milling 2-3 hour, make mean diameter reach 1-5 micron, it is mould that dehydrate can be prepared by 16% Shen piperazine Element CGA-173506 dry suspending agent.
Comparative example 23:16% jingganmycin CGA-173506 dry suspending agent (15:1)
Component: jingganmycin 15%, CGA-173506 1%, lignin sodium naphthalene sulfonate 8%, sodium lignin sulfonate 8%, white carbon 6%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, Kaolin complements to 100%.Soft water 150-200%.Preparation method: Ibid.
Embodiment 36:26% shenqinmycin CGA-173506 water dispersible granules (25:1)
Component: shenqinmycin 25%, CGA-173506 1%, sodium lauryl sulphate 3%, lignin sodium naphthalene sulfonate 6%, anhydrous Sodium sulfate 8%, Kaolin complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 26% shenqinmycin CGA-173506 water dispersible granules.
Comparative example 24:26% Ningnanmycin CGA-173506 water dispersible granules (25:1)
Component: Ningnanmycin 25%, CGA-173506 1%, sodium lauryl sulphate 3%, lignin sodium naphthalene sulfonate 6%, anhydrous Sodium sulfate 8%, Kaolin complements to 100%.Preparation method: ibid.
Embodiment 37:23% shenqinmycin CGA-173506 suspending agent (45:1)
Component: shenqinmycin 22.5%, CGA-173506 0.5%, polycarboxylic acids sodium 4%, fatty alcohol oxygen vinyl Ether 3%, ethylene glycol 5%, XG0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method: each component mixed, through high speed shear mix homogeneously, then passes through sand mill sand milling 2-3 hour, makes to put down All particle diameters reach 1-5 micron, can be prepared by 23% shenqinmycin CGA-173506 suspending agent.
Embodiment 38:20.4% shenqinmycin CGA-173506 dry suspending agent (50:1)
Component: shenqinmycin 20%, CGA-173506 0.4%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 10%, organic Silicon defoaming agent 0.4%, sodium benzoate 0.1%, white carbon 6%, Pulvis Talci complements to 100%.Soft water 150-200%.
Preparation method: add the water of 1.5 2.0 times of preparation amount of preparation, each component is mixed, through high speed shear mixing all Even, then pass through sand mill sand milling 2-3 hour, make mean diameter reach 1-5 micron, it is mould that dehydrate can be prepared by 20.4% Shen piperazine Element CGA-173506 dry suspending agent.
Comparative example 25:20.4% kasugarnycin CGA-173506 dry suspending agent (50:1)
Component: kasugarnycin 20%, CGA-173506 0.4%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 10%, organic Silicon defoaming agent 0.4%, sodium benzoate 0.1%, white carbon 6%, Pulvis Talci complements to 100%.Soft water 150-200%.Preparation side Method: ibid.
Embodiment 39:20.2% shenqinmycin CGA-173506 wettable powder (100:1)
Component: shenqinmycin 20%, CGA-173506 0.2%, dodecylbenzene sodium sulfonate 3%, lignin sodium naphthalene sulfonate 6%, White carbon 6%, Pulvis Talci complements to 100%.
Preparation method: by each component mix homogeneously, first pass through mechanical activation comminution, then after comminution by gas stream, mix homogeneously, i.e. 20.2% shenqinmycin CGA-173506 wettable powder can be prepared.
Comparative example 26:20.2% polyoxin CGA-173506 wettable powder (100:1)
Component: polyoxin 20%, CGA-173506 0.2%, dodecylbenzene sodium sulfonate 3%, lignin sodium naphthalene sulfonate 6%, White carbon 6%, Pulvis Talci complements to 100%.
Preparation method: ibid.
Comparative example 27:20.2% tetramycin CGA-173506 wettable powder (100:1)
Component: tetramycin 20%, CGA-173506 0.2%, dodecylbenzene sodium sulfonate 3%, lignin sodium naphthalene sulfonate 6%, White carbon 6%, Pulvis Talci complements to 100%.Preparation method: ibid.
Four, shenqinmycin compounds with phosethyl-Al
Embodiment 40:20.2% shenqinmycin phosethyl-Al wettable powder (1:100)
Component: shenqinmycin 0.2%, phosethyl-Al 20%, dodecylbenzene sodium sulfonate 3%, lignin sodium naphthalene sulfonate 6%, white carbon 10%, Kaolin complements to 100%.
Preparation method: by each component mix homogeneously, first pass through mechanical activation comminution, then after comminution by gas stream, mix homogeneously, i.e. 20.2% shenqinmycin phosethyl-Al wettable powder can be prepared.
Comparative example 28:20.2% polyoxin phosethyl-Al wettable powder (1:100)
Component: polyoxin 0.2%, phosethyl-Al 20%, dodecylbenzene sodium sulfonate 3%, lignin sodium naphthalene sulfonate 6%, white carbon 10%, Kaolin complements to 100%.Preparation method: ibid.
Embodiment 41:16.8% shenqinmycin phosethyl-Al water dispersible granules (1:55)
Component: shenqinmycin 0.3%, phosethyl-Al 16.5%, sodium lauryl sulphate 4%, polycarboxylic acids sodium 4%, nothing Aqueous sodium persulfate 15%, Pulvis Talci complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 16.8% shenqinmycin phosethyl-Al water dispersible granules.
Embodiment 42:25.5% shenqinmycin phosethyl-Al wettable powder (1:50)
Component: shenqinmycin 0.5%, phosethyl-Al 25%, dodecylbenzene sodium sulfonate 5%, lignin sodium naphthalene sulfonate 8%, white carbon 10%, precipitated calcium carbonate complements to 100%.
Preparation method: by each component mix homogeneously, first pass through mechanical activation comminution, then after comminution by gas stream, mix homogeneously, i.e. 25.5% shenqinmycin phosethyl-Al wettable powder can be prepared.
Comparative example 29:25.5% kasugarnycin phosethyl-Al wettable powder (1:50)
Component: kasugarnycin 0.5%, phosethyl-Al 25%, dodecylbenzene sodium sulfonate 5%, lignin sodium naphthalene sulfonate 8%, white carbon 10%, precipitated calcium carbonate complements to 100%.Preparation method: ibid.
Embodiment 43:35% shenqinmycin phosethyl-Al dry suspending agent (1:34)
Component: shenqinmycin 1%, phosethyl-Al 34%, lignin sodium naphthalene sulfonate 10%, sodium lignin sulfonate 6%, in vain White carbon black 12%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, Pulvis Talci complements to 100%.Soft water 150-200%.
Preparation method: add the water of 1.5 2.0 times of preparation amount of preparation, each component is mixed, through high speed shear mixing all Even, then pass through sand mill sand milling 2-3 hour, make mean diameter reach 1-5 micron, it is mould that dehydrate can be prepared by 35% Shen piperazine Element phosethyl-Al dry suspending agent.
Embodiment 44:26% shenqinmycin phosethyl-Al water dispersible granules (1:25)
Component: shenqinmycin 1%, phosethyl-Al 25%, dodecylbenzene sodium sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous Sodium sulfate 15%, Pulvis Talci complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 26% shenqinmycin phosethyl-Al water dispersible granules.
Comparative example 30:26% jingganmycin phosethyl-Al water dispersible granules (1:25)
Component: jingganmycin 1%, phosethyl-Al 25%, dodecylbenzene sodium sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous Sodium sulfate 15%, Pulvis Talci complements to 100%.Preparation method: ibid.
Embodiment 45:32% shenqinmycin phosethyl-Al wettable powder (1:15)
Component: shenqinmycin 2%, phosethyl-Al 30%, dodecylbenzene sodium sulfonate 3%, polycarboxylic acids sodium 5%, Linesless charcoal Black 12%, Pulvis Talci complements to 100%.
Preparation method: by each component mix homogeneously, first pass through mechanical activation comminution, then after comminution by gas stream, mix homogeneously, i.e. 32% shenqinmycin phosethyl-Al wettable powder can be prepared.
Embodiment 46:20% shenqinmycin phosethyl-Al dry suspending agent (1:1)
Component: shenqinmycin 10%, phosethyl-Al 10%, polycarboxylic acids sodium 5%, sodium lignin sulfonate 10%, organosilicon Defoamer 0.4%, sodium benzoate 0.1%, white carbon 8%, Kaolin complements to 100%.Soft water 150-200%.
Preparation method: add the water of 1.5 2.0 times of preparation amount of preparation, each component is mixed, through high speed shear mixing all Even, then pass through sand mill sand milling 2-3 hour, make mean diameter reach 1-5 micron, it is mould that dehydrate can be prepared by 25% Shen piperazine Element phosethyl-Al dry suspending agent.
Comparative example 31:20% tetramycin phosethyl-Al dry suspending agent (1:1)
Component: tetramycin 10%, phosethyl-Al 10%, polycarboxylic acids sodium 5%, sodium lignin sulfonate 10%, organosilicon Defoamer 0.4%, sodium benzoate 0.1%, white carbon 8%, Kaolin complements to 100%.Soft water 150-200%.Preparation method: Ibid.
Comparative example 32:20% kasugarnycin phosethyl-Al dry suspending agent (1:1)
Component: kasugarnycin 10%, phosethyl-Al 10%, polycarboxylic acids sodium 5%, sodium lignin sulfonate 10%, organosilicon Defoamer 0.4%, sodium benzoate 0.1%, white carbon 8%, Kaolin complements to 100%.Soft water 150-200%.Preparation method: Ibid.
Embodiment 47:21% shenqinmycin phosethyl-Al water dispersible granules (19:2)
Component: shenqinmycin 19%, phosethyl-Al 2%, polycarboxylic acids sodium 4%, sodium lauryl sulphate 4%, anhydrous sulfur Acid sodium 10%, Pulvis Talci complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 21% shenqinmycin phosethyl-Al water dispersible granules.
Embodiment 48:16% shenqinmycin phosethyl-Al suspending agent (15:1)
Component: shenqinmycin 15%, phosethyl-Al 1%, polycarboxylic acids sodium 4%, fatty alcohol oxygen vinyl Ether 3%, ethylene glycol 5%, XG0.35%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method: each component mixed, through high speed shear mix homogeneously, then passes through sand mill sand milling 2-3 hour, makes to put down All particle diameters reach 1-5 micron, can be prepared by 16% shenqinmycin phosethyl-Al suspending agent.
Embodiment 49:26% shenqinmycin phosethyl-Al water dispersible granules (25:1)
Component: shenqinmycin 25%, phosethyl-Al 1%, dodecylbenzene sodium sulfonate 3%, lignin sodium naphthalene sulfonate 6%, anhydrous sodium sulfate 6%, Kaolin complements to 100%.
Preparation method: each component is mixed, through mechanical activation comminution, then through comminution by gas stream, mix homogeneously, then through wet granulation also Dry, can be prepared by 26% shenqinmycin phosethyl-Al water dispersible granules.
Comparative example 33:26% tetramycin phosethyl-Al water dispersible granules (25:1)
Component: shenqinmycin 25%, phosethyl-Al 1%, dodecylbenzene sodium sulfonate 3%, lignin sodium naphthalene sulfonate 6%, anhydrous sodium sulfate 6%, Kaolin complements to 100%.Preparation method: ibid.
Embodiment 50:23% shenqinmycin phosethyl-Al suspending agent (45:1)
Component: shenqinmycin 22.5%, phosethyl-Al 0.5%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 3%, fat Alcohol oxygen vinyl Ether 3%, ethylene glycol 5%, XG0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method: each component mixed, through high speed shear mix homogeneously, then passes through sand mill sand milling 2-3 hour, makes to put down All particle diameters reach 1-5 micron, can be prepared by 23% shenqinmycin phosethyl-Al suspending agent.
Embodiment 51:20.4% shenqinmycin phosethyl-Al dry suspending agent (50:1)
Component: shenqinmycin 20%, phosethyl-Al 0.4%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 8%, Organic silicon defoamer 0.4%, sodium benzoate 0.1%, white carbon 6%, Pulvis Talci complements to 100%.Soft water 150-200%.
Preparation method: add the water of 1.5 2.0 times of preparation amount of preparation, each component is mixed, through high speed shear mixing all Even, then pass through sand mill sand milling 2-3 hour, make mean diameter reach 1-5 micron, it is mould that dehydrate can be prepared by 20.4% Shen piperazine Element phosethyl-Al dry suspending agent.
Comparative example 34:20.4% kasugarnycin phosethyl-Al dry suspending agent (50:1)
Component: kasugarnycin 20%, phosethyl-Al 0.4%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 8%, Organic silicon defoamer 0.4%, sodium benzoate 0.1%, white carbon 6%, Pulvis Talci complements to 100%.Soft water 150-200%.System Preparation Method: ibid.
Embodiment 52:20.2% shenqinmycin phosethyl-Al wettable powder (100:1)
Component: shenqinmycin 20%, phosethyl-Al 0.2%, dodecylbenzene sodium sulfonate 3%, naphthalene sulfonate formaldehyde contracts Compound 6%, white carbon 6%, Kaolin complements to 100%.
Preparation method: by each component mix homogeneously, first pass through mechanical activation comminution, then after comminution by gas stream, mix homogeneously, i.e. 20.2% shenqinmycin phosethyl-Al wettable powder can be prepared.
Comparative example 35:20.2% polyoxin phosethyl-Al wettable powder (100:1)
Component: shenqinmycin 20%, phosethyl-Al 0.2%, dodecylbenzene sodium sulfonate 3%, naphthalene sulfonate formaldehyde contracts Compound 6%, white carbon 6%, Kaolin complements to 100%.Preparation method: ibid.
Finding through experiment, the cyazofamid in above example, mould spirit, CGA-173506 or phosethyl-Al can mutually replace In generation, to form new embodiment.No longer carry out this repeating to enumerate, it is noted that for the ordinary skill people of the art For Yuan, on the premise of without departing from principle of the present invention, it is also possible to make some improvements and modifications, these improvements and modifications Also should be regarded as protection scope of the present invention.
The pesticidal preparations prepared by above-described embodiment, comparative example prevents and treats cucumber downy mildew, tomato late blight test.
1, test processes: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination Testing dosing and set three concentration for the treatment of respectively according to the difference of each composition, comparison medicament is commercially available pesticide 20% cyanogen frost respectively Azole suspending agent, 1% shenqinmycin SC and blank clean water experiment.
2, test method: each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after investigating before dispenser and preventing and treating Method is: investigate with blank incidence classification, and every community diagonal samples, and often clicks 10 strains, records total strain number, diseased plant number With sick progression.
The cucumber downy mildew field investigation standard following stage division of employing:
0 grade: anosis；
1 grade: lesion area accounts for less than the 5% of whole leaf area；
3 grades: lesion area accounts for the 5%-10% of whole leaf area；
5 grades: lesion area accounts for the 11%-25% of whole leaf area；
7 grades: lesion area accounts for the 26%-50% of whole leaf area；
9 grades: lesion area accounts for more than the 50% of whole leaf area；
Result of the test see table 5,6:
Table 5 shenqinmycin (A) and the compounding prevention effect to cucumber downy mildew of cyazofamid (B)
Table 5 field efficacy result shows, in the range of weight ratio 1:100-100:1, shenqinmycin is compounding with cyazofamid to be increased Effect effect is obvious, in the range of particularly 1:50-50:1, in the range of especially 1:25-25:1, has cucumber downy mildew excellent Prevention effect, prevention effect is better than single dose kind.
The tomato late blight field investigation standard following stage division of employing:
0 grade: anosis；
1 grade: on indivedual blades, have indivedual scab；
3 grades: 1/3 with the ill speckle of lower blade；
5 grades: 1/3-1/2 blade has scab；
7 grades: nearly all blade has scab；
9 grades: all blade goes rotten almost without green portion；
Other statistics, experimental techniques are ibid.
The prevention effect of tomato late blight is compared by table 6 embodiment with comparative example
Inventor finds that other antibiotics antibacterial compounds with cyazofamid, in the range of weight ratio 1:100-100:1 also There is certain Complex synergism.Because length is limited, do not enumerate, only with the most representational kasugarnycin, many anti-mildews Element, Ningnanmycin construct comparative example and carry out preventive effect contrast, as table 6 field efficacy result shows, in weight ratio 1 with embodiment: In the range of 100-100:1, shenqinmycin is obvious with cyazofamid Complex synergism, is better than other antibiotics antibacterial and cyanogen frost The prevention effect that azoles is compounding.Particularly in the range of 1:50-50:1, in the range of especially 1:25-25:1, shenqinmycin and cyanogen frost Azoles Complex synergism is obvious, the prevention effect to plant disease, is better than other antibiotics antibacterial with cyazofamid compounding Prevention effect.
The pesticidal preparations prepared by above-described embodiment, comparative example prevents and treats root rotof flax, root rot test.
1, test processes: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination Testing dosing and set three concentration for the treatment of respectively according to the difference of each composition, comparison medicament is that commercially available pesticide 30% is mould respectively Lingshui Spring agent, 1% shenqinmycin SC and blank clean water experiment.
2, test method: each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after investigating before dispenser and preventing and treating Method is: investigate with blank incidence classification, and every community diagonal samples, and often clicks 10 strains, records total strain number, diseased plant number With sick progression.
Use following stage division:
0 grade: basal part of stem and master, fibrous root are all without scab；
1 grade: have a small amount of scab on basal part of stem and main root, lesion area accounts for stem and less than 1/4th of the root gross area；
3 grades: on basal part of stem and main root, have more scab, lesion area account for stem and 1/4th of the root gross area and two/ One；
5 grades: on basal part of stem and main root, have more scab, lesion area account for stem and 1/2nd of the root gross area and four/ Three；
7 grades: on basal part of stem and main root, scab is in flakes, but root system is the most dead；
9 grades: root system is downright bad, above-ground plant parts is wilted close to dead or dead；
Result of the test see table 7,8:
Table 7 shenqinmycin (A) and the compounding prevention effect to root rotof flax of mould spirit (B)
Table 7 field efficacy result shows, in the range of weight ratio 1:100-100:1, shenqinmycin is compounding with mould spirit to be increased Effect effect is obvious, in the range of particularly 1:50-50:1, in the range of especially 1:25-25:1, has root rotof flax excellent Prevention effect, prevention effect is better than single dose kind.
The prevention effect of root rot is compared by table 8 embodiment with comparative example
Inventor finds that other antibiotics antibacterial compounds with mould spirit, in the range of weight ratio 1:100-100:1 also There is certain Complex synergism.Because length is limited, do not enumerate, only the most mould with the most representational polyoxin, Chinese parasol tree Element constructs comparative example and carries out preventive effect contrast, as table 8 field efficacy result shows, in weight ratio 1:100-100:1 with embodiment In the range of, shenqinmycin is obvious with mould spirit Complex synergism, is better than other antibiotics antibacterial compounding with mould spirit Prevention effect.Particularly in the range of 1:50-50:1, in the range of especially 1:25-25:1, shenqinmycin is compounding with mould spirit to be increased Effect effect is obvious, the prevention effect to plant disease, is better than the prevention effect that other antibiotics antibacterial is compounding with mould spirit.
The pesticidal preparations prepared by above-described embodiment, comparative example prevents and treats grey mould fruit rot of strawberry, mango anthracnose test.
1, test processes: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination Testing dosing and set three concentration for the treatment of respectively according to the difference of each composition, comparison medicament is that commercially available pesticide 25g/l coughs up respectively Bacterium nitrile wettable powder, 1% shenqinmycin SC and blank clean water experiment.
2, test method: each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after investigating before dispenser and preventing and treating Method is: investigate with blank incidence classification, and every community diagonal samples, and often clicks 10 strains, records total strain number, diseased plant number With sick progression.
Use following stage division:
0 grade: anosis；
1 grade: lesion area accounts for less than the 5% of whole leaf area；
3 grades: lesion area accounts for the 6%-15% of whole leaf area；
5 grades: lesion area accounts for the 16%-25% of whole leaf area；
7 grades: lesion area accounts for below the 26%-50% of whole leaf area；
9 grades: lesion area accounts for more than the 50% of whole leaf area；
Result of the test see table 9,10:
Table 9 shenqinmycin (A) and the compounding prevention effect to grey mould fruit rot of strawberry of CGA-173506 (B)
Table 9 field efficacy result shows, in the range of weight ratio 1:100-100:1, shenqinmycin is compounding with CGA-173506 to be increased Effect effect is obvious, in the range of particularly 1:50-50:1, in the range of especially 1:25-25:1, has grey mould fruit rot of strawberry excellent Prevention effect, prevention effect is better than single dose kind.
The prevention effect of mango anthracnose is compared by table 10 embodiment with comparative example
Inventor finds that other antibiotics antibacterial compounds with CGA-173506, in the range of weight ratio 1:100-100:1 also There is certain Complex synergism.Because length is limited, do not enumerate, only with the most representational kasugarnycin, many anti-mildews Element, Ningnanmycin, jingganmycin, tetramycin construct comparative example and carry out preventive effect contrast, such as table 10 field efficacy with embodiment Result shows, in the range of weight ratio 1:100-100:1, shenqinmycin is obvious with CGA-173506 Complex synergism, is better than other The prevention effect that antibiotics antibacterial is compounding with CGA-173506.Particularly in the range of 1:50-50:1, especially 1:25-25:1 In the range of, shenqinmycin is obvious with CGA-173506 Complex synergism, and the prevention effect to plant disease is better than other antibiotics The prevention effect that antibacterial is compounding with CGA-173506.
The pesticidal preparations prepared by above-described embodiment, comparative example prevents and treats Chinese cabbage downy mildew, black shank test.
1, test processes: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination Testing dosing and set three concentration for the treatment of respectively according to the difference of each composition, comparison medicament is commercially available pesticide 40% 3 second respectively Phosphonic acids aluminum wettable powder, 1% shenqinmycin SC and blank clean water experiment.
2, test method: each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after investigating before dispenser and preventing and treating Method is: investigate with blank incidence classification, and every community diagonal samples, and often clicks 10 strains, records total strain number, diseased plant number With sick progression.
Use following stage division:
0 grade: anosis；
1 grade: lesion area accounts for less than the 5% of whole leaf area；
3 grades: lesion area accounts for the 5%-10% of whole leaf area；
5 grades: lesion area accounts for the 11%-25% of whole leaf area；
7 grades: lesion area accounts for the 26%-50% of whole leaf area；
9 grades: lesion area accounts for more than the 50% of whole leaf area；
Result of the test see table 11,12:
Table 11 shenqinmycin (A) and the compounding prevention effect to Chinese cabbage downy mildew of phosethyl-Al (B)
Table 11 field efficacy result shows, in the range of weight ratio 1:100-100:1, shenqinmycin is multiple with phosethyl-Al Join synergistic effect obvious, in the range of particularly 1:50-50:1, in the range of especially 1:25-25:1, Chinese cabbage downy mildew is had excellent Good prevention effect, prevention effect is better than single dose kind.
Black shank investigation standard:
0 grade: Herb is anosis；
1 grade: stem's scab is less than 1/2nd of stem girth, or half one lower blade is the most wilting, or bottom minority leaf There is scab in sheet；
3 grades: stem's scab exceedes 1/2nd of stem girth, or half one blade is the most wilting；
5 grades: stem's scab is around stem girth, or 2/3rds is wilting with blade；
7 grades: the whole blade of diseased plant is wilting or withered.
The prevention effect of black shank is compared by table 12 embodiment with comparative example
Inventor finds that other antibiotics antibacterial compounds with phosethyl-Al, in weight ratio 1:100-100:1 scope The most also there is certain Complex synergism.Because length is limited, do not enumerate, only with the most representational kasugarnycin, many Antimycin, jingganmycin, tetramycin construct comparative example and carry out preventive effect contrast, such as table 12 field efficacy result table with embodiment Bright, in the range of weight ratio 1:100-100:1, shenqinmycin is obvious with phosethyl-Al Complex synergism, is better than other and resists The prevention effect that raw element series bactericidal agent is compounding with phosethyl-Al.Particularly in the range of 1:50-50:1, especially 1:25-25: In the range of 1, shenqinmycin is obvious with phosethyl-Al Complex synergism, the prevention effect to plant disease, is better than other and resists The prevention effect that raw element series bactericidal agent is compounding with phosethyl-Al.
Inventor finds that shenqinmycin and cyazofamid, Shen shenqinmycin and mould spirit, shenqinmycin and CGA-173506, Shen piperazine are mould Element is compounding with three second aluminum phosphate has obvious potentiation to plant disease.In identical proportion 1:100-100:1, In the range of particularly 1:50-50:1, in the range of especially 1:25-25:1, find shenqinmycin and cyazofamid, shenqinmycin Compounding with mould spirit, shenqinmycin and CGA-173506, shenqinmycin and three second aluminum phosphate also have obvious synergistic effect.Due to a piece Limited, it is not repeated.
The above is the preferred embodiment of the present invention, it is noted that for those skilled in the art For, on the premise of without departing from principle of the present invention, it is also possible to make some improvements and modifications, these improvements and modifications are also Should be regarded as protection scope of the present invention.

Claims (10)

1. a bactericidal composition, it is characterised in that containing effective ingredient shenqinmycin and active bactericide, described shenqinmycin It is 1:100～100:1 with the weight ratio of active bactericide.
Bactericidal composition the most according to claim 1, it is characterised in that described shenqinmycin and the weight of active bactericide Ratio is 1:50～50:1.
Bactericidal composition the most according to claim 2, it is characterised in that described shenqinmycin and the weight of active bactericide Ratio is 1:25～25:1.
4. according to described bactericidal composition arbitrary in claims 1 to 3, it is characterised in that described active bactericide is cyanogen frost Azoles, CGA-173506 or phosethyl-Al.
5. according to described bactericidal composition arbitrary in Claims 1-4, it is characterised in that described bactericidal composition also contains There is pesticidal preparations auxiliary element, to make the dosage form being suitable for agriculturally using.
Bactericidal composition the most according to claim 5, it is characterised in that described pesticidal preparations auxiliary element is solvent, breast One or more in agent, dispersant, stabilizer, antifreezing agent or wetting agent.
Bactericidal composition the most according to claim 5, it is characterised in that the dosage form of described bactericidal composition be suspending agent, Dry suspending agent, water dispersible granules, wettable powder, seed treatment or suspension seed-coating agent.
8. claim 1 to 7 arbitrary described bactericidal composition application in the pesticidal preparations of preparation controlling plant diseases.
The application of bactericidal composition the most according to claim 8, it is characterised in that prevent and treat rice sheath blight disease, rice in preparation Pestilence, false smut, wheat rust, powdery mildew, root rotof flax, wheat sharp eyespot, rice sheath blight disease, root rot, Brassica campestris L Sclerotiniose, maize head smut, capsicum wilt, cucurbits fusarium wilt pesticidal preparations in application.
10. the method that the bactericidal composition described in claim 1 to 7 is applied to controlling disease, it is characterised in that active component Shen Piperazine mycin and active bactericide are used with above-mentioned weight ratio, it uses after being mixed in proportion, use the most simultaneously or by than Example is used successively.
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