CN103300053B - Bactericidal composition containing pyraclostrobin - Google Patents
Bactericidal composition containing pyraclostrobin Download PDFInfo
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- CN103300053B CN103300053B CN201310233196.4A CN201310233196A CN103300053B CN 103300053 B CN103300053 B CN 103300053B CN 201310233196 A CN201310233196 A CN 201310233196A CN 103300053 B CN103300053 B CN 103300053B
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Abstract
The invention discloses a bactericidal composition containing pyraclostrobin. The bactericidal composition comprises the active ingredients, namely, pyraclostrobin and phosethyl-al, wherein the weight ratio of the pyraclostrobin to the phosethyl-al is (1:15) to (10:1). The composition can be prepared into agriculturally allowable water dispersible granules, wettable powder and suspending agent. The bactericidal composition is reasonable in components, good in sterilization effect, and low in drug cost; and the activity and the sterilization effect of the bactericidal composition are not simply overlapped by the activity of each component. Compared with an existing single preparation, the bactericidal composition has a significant synergism and good crop safety besides a significant sterilization effect, accords with the safety requirements of a pesticide preparation, and has significant prevention and treatment effects on downy mildew, epidemic diseases and leaf spot diseases of crops.
Description
Technical field
The bactericidal composition of patent application of the present invention to be invention and created name be a kind of pyraclostrobin-containing, application number is 201210084073.4, and the applying date is the divisional application of 20120328 patent applications.
The present invention relates to a kind of bactericidal composition of pyraclostrobin-containing, particularly a kind of pyraclostrobin is as the first active component, and cyazofamid or phosethyl-Al, as the bactericidal composition of the second active component, belong to technical field of compounded pesticide.
Background technology
Pyraclostrobin, has another name called pyraclostrobin, English common name: Pyraclostrobin, chemical name: N-{2-[1-(4-chlorphenyl)-1H-pyrazole-3-yl oxygen methyl] phenyl } (N-methoxyl group) carbamate.Pyraclostrobin belongs to methoxy acrylic bactericide, mainly acts on the cytochrome b el compound in the mitochondrial respiratory chain of fungi, stops electron transmission thus Antifungi growth.Can prevent and kill off all types of fungal pathogens, fungicidal spectrum is wide, to various crop, comprise wheat class, grape, fruit tree, vegetables multiple diseases effective, be mainly used in preventing and treating grape powdery mildew, downy mildew; Wheat powdery mildew, rust; Leaf rust of barley; The black leaf cecospora spot of banana; Tomato morning, late blight etc.Although this bactericide mechanism of action is unique, the same with other bactericide, pyraclostrobin is also unable to escape the misfortune of resistance, and use cost is high.
Oomycete belongs to Mycophyta, and be a guiding principle of Mastigomycotina, Oomycete mainly contains pythium spp, phytophthora, downy mildew etc., often causes the downy mildew of crop, epidemic disease, damping off etc.The medicament preventing and treating this kind of disease conventional has dimethomorph, cyazofamid, phosethyl-Al agricultural chemicals and mixture thereof.
Cyazofamid, the chloro-2-cyano group of English common name cyazofamid, chemical name: 4--N, N-dimethyl-5-p-methylphenyl imidazoles-1-sulfonamide.Cyazofamid is sulphaguanidine azoles fungicide.Super protective fungicide, also has certain interior suction and therapeutic activity.To the plasmodiophora brassica bacteria of Oomycete fungal as downy mildew, Pseudo peronospara, phytophthora, pythium spp and Plasmodiophoromycetes, there is very high biologically active.Controlling object downy mildew, epidemic disease are as cucumber downy mildew, downy mildew of garpe, tomato late blight, potato late blight etc.
Phosethyl-Al, has another name called Yimeiling, fosetyl aluminium, and mould epidemic disease is clean, Methylpartricin Sodium Laurylsulfate, mould spirit, English common name phosethyl-Al, chemical name: three-(ethylphosphonic acid) aluminium.Phosethyl-Al is systemic fungicide, to disease tool special efficacy caused by oomycetes, can conduct up and down in plant corpus, has protection and therapeutic action.
Above-mentioned cyazofamid, phosethyl-Al are for preventing and treating the special agricultural chemicals of Oomycete, the speed that pathogen produces resistance to it is also very fast, use separately dosage increasing, resistance is used to strengthen year by year for many years, and controlling object is single, poor to diseases control beyond Oomycete, also there is no the report of these two kinds of agricultural chemicals and pyraclostrobin mixture by literature search at present.Inventor is found by test; by pyraclostrobins different for the mechanism of action and cyazofamid or phosethyl-Al composite; there is obvious synergistic effect; treatment, the control efficiency of protection, expanding prevention spectrum can be significantly improved; when oomycetes diseases and the mixing of other fungal diseases occur, a dispenser can doublely be controlled, and reduces spraying times and dosage; reduce cost accounting, to crop safety.
Summary of the invention
The object of the present invention is to provide a kind of component reasonable, synergistic effect is remarkable, and bactericidal effect is good, and drug cost is low, not easily develops immunity to drugs, to the pyraclostrobin-containing of crop safety and the agricultural bactericidal composition of cyazofamid or phosethyl-Al.
Another object of the present invention is to provide the application of above-mentioned composition in control crops downy mildew, epidemic disease, leaf spot.
In order to overcome the defect of existing unitary agent, technical scheme of the present invention solves like this:
A) the first active component pyraclostrobin;
B) the second active component cyazofamid or phosethyl-Al;
The weight ratio of the first active component and the second active component is 1:15 ~ 10:1, is preferably 1:10 ~ 5:1, most preferably is 1:5 ~ 1:1.
The cumulant of the first active component and the second active component is 5% ~ 90% of described composition total weight, is preferably 10% ~ 70%.
Bactericidal composition of the present invention is water dispersible granules, wetting powder, suspending agent according to the preparation formulation that method known in those skilled in the art can be prepared.
For water dispersible granules, those skilled in the art are familiar with the corresponding auxiliary agent of use and complete the present invention very much.Dispersant as in polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate one or more; Wetting agent as in alkyl sulfate, alkylsulfonate, naphthalene sulfonate one or more; Disintegrant as in ammonium sulfate, urea, sucrose, glucose one or more; Binding agent is as one or more in diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose family; Filler as diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay one or more.
To wetting powder, spendable auxiliary agent has: dispersant as in polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate one or more; Wetting agent as in alkyl sulfate, alkylsulfonate, naphthalene sulfonate one or more; Filler as ammonium sulfate, urea, sucrose, glucose, diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay one or more.
To suspending agent, spendable auxiliary agent has: dispersant such as polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate (dispersing agent NNO), Huntsman Corporation of the TERSPERSE 2020(U.S. produce, alkyl naphthalene sulfonic acid salt) in one or more; Emulsifier is as Nongru-700 #(common name: alkyl phenol formaldehyde resin polyoxyethylene ether), agriculture breast 2201, this dish-60#(common name: Span60), Tween-60 #(common name: polyoxyethylene sorbitan stearate), agriculture breast 1601#(common name: triphenoethyl benzene phenol polyoxyethylene polyoxypropylene block polymer), Huntsman Corporation of the TERSPERSE 4894(U.S. produces) in one or more; Wetting agent such as alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, Huntsman Corporation of the TERSPERSE 2500(U.S. produce) in one or more; Thickener as in xanthans, polyvinyl alcohol, bentonite, aluminium-magnesium silicate one or more; Preservative as in formaldehyde, benzoic acid, Sodium Benzoate one or more; Stabilizing agent as in epoxidized soybean oil, epoxychloropropane, triphenyl phosphate one or more; Defoamer is as organic silicon defoamer; Antifreezing agent as ethylene glycol, propane diols, glycerine, urea, inorganic salts as in sodium chloride one or more; Water is deionized water.
Instant component is reasonable, and treatment adds protective effect, and bactericidal effect is good, and drug cost is low, and its activity and bactericidal effect are not the simple superposition of each composition activity, but has significant synergistic effect, good to crop safety, meets the security requirement of pesticidal preparations.The present invention has good control efficiency to control crops downy mildew, epidemic disease, leaf spot.
Embodiment
In order to prevent and treat vegetables in agricultural production, fruit tree downy mildew, inventor has carried out mutual composite synergy with pyraclostrobin and cyazofamid or phosethyl-Al and has studied, and concrete grammar is:
Test adopts bacterium of downy mildew of cucumber (Pseudoperonospora cubensis) to be tested object.First former medicine is mixed with the test medicine of needs, test method is with reference to " People's Republic of China's agricultural industry criteria NY/T1156.3-2006 ".Single dose and each Mixed Pharmacy are arranged 5 variable concentrations gradients, pyraclostrobin and cyazofamid combine, each single dose and Mixed Pharmacy concentration are respectively 0.625 mg/L, 1.25 mg/L, 2.5 mg/L, 5 mg/L, 10 mg/L, pyraclostrobin and phosethyl-Al combine, the concentration of pyraclostrobin single dose and Mixed Pharmacy is respectively 0.625 mg/L, 1.25 mg/L, 2.5 mg/L, 5 mg/L, 10 mg/L, and phosethyl-Al concentration is 2 mg/L, 4 mg/L, 8 mg/L, 16 mg/L, 32 mg/L.Test target bacterium is the bacterium of downy mildew of cucumber blade picking up from from up to down 4 leaf positions, leaf ~ 6, field, with the Pseudoperonospora cubensis sporangium at 4 DEG C of distillation washing lower blade back sides, be made into suspension (concentration controls at every milliliter of 1 × 105 ~ 1 × 107 sporangium), deposit for subsequent use at 4 DEG C; Liquid is evenly sprayed in the healthy leaves back side, after liquid natural air drying, each process blade and blade is gone up dorsad, is emitted in moisture preservation box by after marks for treatment.Test is established and is not made blank containing the process of medicament.Vacuum side of blade is inoculated in ready Fresh spores capsule suspension drop 10 μ L.Every blade inoculation 4, often process is no less than 5 leaves.Protectiveness test is 24h inoculation after chemicals treatment, curative tests 24h inoculation before treatment.Ware lid on inoculation bonnet, is placed in climatic cabinate or has the humidistat of illumination, and alternately, temperature is 17 DEG C ~ 22 DEG C to the continuous illumination in every day/dark 12h, cultivates under the condition of relative moisture more than 90%.Measure depending on process and blank incidence and record lesion diameter, unit is millimeter (mm), calculates control efficiency.By the linear regression analysis between the probit value of control efficiency and the logarithm value of series concentration, obtain the EC of each medicament
50value, calculates the co-toxicity coefficient (CTC) of mixture, evaluates the activity of reagent agent to germ with this by the abundant method of Sun Yun.
The co-toxicity coefficient (CTC value) of mixture is by following formulae discovery:
In formula:
ATI-----mixture actual measurement toxicity index;
S---standard antiseptic agent LC
50, unit is milligrams per liter (mg/L);
The LC of M---mixture
50, unit is milligrams per liter (mg/L);
TTI=TI
a× P
a+ TI
b× P
b, in formula:
The theoretical toxicity index of TTI---mixture;
TI
a---A medicament toxicity index;
P
a---the percentage composition of A medicament in mixture, unit is percentage (%);
TI
b---B medicament toxicity index;
P
b---the percentage composition of B medicament in mixture, unit is percentage (%);
, in formula:
CTC---co-toxicity coefficient;
ATI---mixture actual measurement toxicity index;
The theoretical toxicity index of TTI---mixture;
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 < CTC < 120 show as summation action.
Toxicity Determination result shows: pyraclostrobin is that 1:15 ~ 10:1 is mixed with cyazofamid or phosethyl-Al with weight ratio has good virulence to bacterium of downy mildew of cucumber, all has significant synergistic effect.
In order to prevent and treat the epidemic disease in agricultural production, invent and carried out mutual composite synergy with pyraclostrobin and cyazofamid or phosethyl-Al and study, concrete grammar is:
Subjects is tomato early blight bacterium (Alternaria solani).Former medicine is mixed with the test medicine of needs, test method is with reference to " People's Republic of China's agricultural industry criteria NY/T1156.2-2006 ".Single dose and each Mixed Pharmacy are arranged 5 variable concentrations gradients, pyraclostrobin and cyazofamid combine, each single dose and Mixed Pharmacy concentration are respectively 0.625 mg/L, 1.25 mg/L, 2.5 mg/L, 5 mg/L, 10 mg/L, pyraclostrobin and phosethyl-Al combine, the concentration of pyraclostrobin single dose and Mixed Pharmacy is respectively 0.625 mg/L, 1.25 mg/L, 2.5 mg/L, 5 mg/L, 10 mg/L, and phosethyl-Al concentration is 2 mg/L, 4 mg/L, 8 mg/L, 16 mg/L, 32 mg/L.If clear water contrasts, repeat 3 times.Adopt mycelial growth rate method, by cultured pathogen, be aseptically 5 mm card punch with diameter, cut bacterium cake from colony edge, with transfer needle, bacterium cake is connected on the culture medium flat plate of different agents concentration, put in 26 DEG C of incubators and cultivate 72h; Check colony diameter, calculate each chemicals treatment and suppress the percentage of mycelial growth, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC of each medicament
50value, calculate the co-toxicity coefficient (CTC) of mixture by the abundant method of Sun Yun, computing formula, with downy mildew toxicity test, evaluates the activity of reagent agent to germ with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 < CTC < 120 show as summation action.
Toxicity Determination result shows: pyraclostrobin is that 1:15 ~ 10:1 is mixed with cyazofamid or phosethyl-Al with weight ratio has good virulence to early blight of tomato, all has significant synergistic effect.
In order to prevent and treat the crop leaf spot in agricultural production, inventor has carried out mutual composite synergy with pyraclostrobin and cyazofamid or phosethyl-Al and has studied, and concrete grammar is:
Subjects is, in sigatoka (Sigatokadiseaseofbanana), the Cercospora leaf spot (Cercosproa musae) the most general, harm is the most serious occurs.Former medicine is mixed with the test medicine of needs.First single dose and each Mixed Pharmacy are arranged 5 variable concentrations gradients (on the basis of preliminary experiment result, different with proportioning according to different agents combination, concentration gradient arranges also different, bacteriostasis rate sets by geometric progression in the scope of 5% ~ 90%, pyraclostrobin single dose and and cyazofamid, phosethyl-Al Mixed Pharmacy concentration be respectively 1 mg/L, 2 mg/L, 4 mg/L, 8 mg/L, 16 mg/L, cyazofamid and phosethyl-Al concentration are respectively: 2 mg/L, 4 mg/L, 8 mg/L, 16 mg/L, 32 mg/L).If clear water contrasts, repeat 3 times.Within 2011, select the middle part any of several broadleaf plants leaf of initial morbidity at Nan Tang town, gaozhou,guangdong city morbidity Jiao Yuan, test with the new scab (about 5.0 mm × 1.0 mm) formed of ink pens mark, each repeating label 50 scabs, evenly spray the positive and negative at mark any of several broadleaf plants leaf by each treatment agent.Within 10 days, investigate respectively before spray medicine and after spray medicine and record scab length, calculating scab length increment and fungistatic effect, regression analysis is carried out to the fungistatic effect of different disposal, and calculates EC
50value.Calculate the co-toxicity coefficient (CTC) of mixture by the abundant method of Sun Yun, computing formula, with downy mildew toxicity test, evaluates the activity of reagent agent to germ with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 < CTC < 120 show as summation action.
Toxicity Determination result shows: pyraclostrobin is that 1:15 ~ 10:1 is mixed with cyazofamid or phosethyl-Al with weight ratio has good virulence to sigatoka, all has significant synergistic effect.
Below in conjunction with embodiment, content of the present invention is described further:
Example of formulations 1
Take 12% pyraclostrobin, 36% cyazofamid, 4% Morwet D-425(alkyl naphthalene sulfonic acid condensation polymer sodium salt, Akzo Nobel N.V. produces), 3% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 2%K-12(lauryl sodium sulfate), 3% corn starch, 5% sucrose, kaolin adds to 100% weight portion.Namely the method for above-mentioned raw materials frequent regulation water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step produce 48% pyraclostrobin cyazofamid water dispersible granules.
Example of formulations 2
Take 20% pyraclostrobin, 20% cyazofamid, 4% TERSPERSE 2700(polycarboxylate, Huntsman Corporation of the U.S. produces), 2% dispersing agent NNO, 2% Nekal BX, 2%K-12,3% corn starch, 5% sulphur ammonium, attapulgite add to 100% weight portion.Namely the method for above-mentioned raw materials frequent regulation water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step produce 40% pyraclostrobin cyazofamid water dispersible granules.
Example of formulations 3
Take 15% pyraclostrobin, 75% phosethyl-Al, 1% TERSPERSE 2700,1% dispersing agent NNO, 1% Nekal BX, 1%K-12,1% carboxymethyl cellulose, diatomite adds to 100% weight portion.Namely the method for above-mentioned raw materials frequent regulation water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step produce 90% pyraclostrobin phosethyl-Al water dispersible granules.
Example of formulations 4
Take 7% pyraclostrobin, 56% phosethyl-Al, 3% TERSPERSE 2700,3% dispersing agent NNO, 2% Nekal BX, 2%K-12,1% carboxymethyl cellulose, 5% urea, diatomite adds to 100% weight portion.Namely the method for above-mentioned raw materials frequent regulation water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step produce 63% pyraclostrobin phosethyl-Al water dispersible granules.
Example of formulations 5
Take 40% pyraclostrobin, 4% cyazofamid, 5% calcium lignosulfonate, 2% Nekal BX, 2%K-12,5% White Carbon black, kaolin adds to 100% weight portion.Above-mentioned raw materials, through mixing, obtains 44% pyraclostrobin cyazofamid wetting powder after air-flow crushing.
Example of formulations 6
Take 27% pyraclostrobin, 9% cyazofamid, 4% calcium lignosulfonate, 3% TERSPERSE 2700,2% Nekal BX, 2%K-12,3% White Carbon black, fine particle calcium carbonate add to 100% weight portion.Above-mentioned raw materials, through mixing, obtains 36% pyraclostrobin cyazofamid wetting powder after air-flow crushing.
Example of formulations 7
Take 5% pyraclostrobin, 75% phosethyl-Al, 3% calcium lignosulfonate, 3% dispersing agent NNO, 1% Nekal BX, 1%K-12, attapulgite add to 100% weight portion.Above-mentioned raw materials, through mixing, obtains 80% pyraclostrobin phosethyl-Al wetting powder after air-flow crushing.
Example of formulations 8
Take 13% pyraclostrobin, 52% phosethyl-Al, 4% calcium lignosulfonate, 3% TERSPERSE 2700,2% Nekal BX, 2%K-12,3% White Carbon black, fine particle calcium carbonate add to 100% weight portion.Above-mentioned raw materials, through mixing, obtains 65% pyraclostrobin phosethyl-Al wetting powder after air-flow crushing.
Example of formulations 9
Take 20% pyraclostrobin, 10% cyazofamid, Huntsman Corporation of the 4%TERSPERSE 4894(U.S. produce), Huntsman Corporation of the 1.5%TERSPERSE 2500(U.S. produces), Huntsman Corporation of the 2%TERSPERSE 2020(U.S. produces), 1% aluminium-magnesium silicate, 0.2% xanthans, 5% ethylene glycol, 0.5% formaldehyde, 0.5% silicone defoaming agent (trade name: s-29 Nanjing "four news" (new ideas applied chemistry product Company), 2% triphenyl phosphate, deionized water add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear dispersion 30min, with 30% pyraclostrobin cyazofamid suspending agent obtained after sand mill sand milling.
Example of formulations 10
Take 4% pyraclostrobin, 6% cyazofamid, 5%TERSPERSE 4894,2%TERSPERSE 2500,3%TERSPERSE 2020,0.2% xanthans, 5% propane diols, 0.5% benzoic acid, 1% epoxychloropropane, 0.5% silicone defoaming agent, 1% epoxychloropropane, deionized water add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear dispersion 30min, with 10% pyraclostrobin cyazofamid suspending agent obtained after sand mill sand milling.
Example of formulations 11
Take 13% pyraclostrobin, 26% phosethyl-Al, this dish of 2%TERSPERSE 2020,2%-60#, 2% Tween-60 #, 1%TERSPERSE 2500,0.5% white carbon, 0.3% xanthans, 5% ethylene glycol, 0.5% benzoic acid, 0.5% silicone defoaming agent, 1% epoxychloropropane, deionized water add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear dispersion 30min, with 39% pyraclostrobin phosethyl-Al suspending agent obtained after sand mill sand milling.
Example of formulations 12
Take 6% pyraclostrobin, 36% phosethyl-Al, 1%TERSPERSE 2020,3% agriculture breast 1601#, 2% agriculture breast 2201#, 1%TERSPERSE 2500,0.3% xanthans, 5% ethylene glycol, 0.5% formaldehyde, 0.5% silicone defoaming agent, 1% epoxychloropropane, deionized water add to 100% weight portion.High speed shear dispersion 30min, with 42% pyraclostrobin phosethyl-Al suspending agent obtained after sand mill sand milling.
Biological Examples 1: prevent and treat downy mildew of garpe field trial
Downy mildew is one of important disease of grape, blade of mainly causing harm, also cause harm inflorescence and young fruit.Within 2011, carried out example of formulations 9(30% pyraclostrobin cyazofamid suspending agent in Yantai, Shandong), example of formulations 8(65% pyraclostrobin phosethyl-Al wetting powder) prevent and treat downy mildew of garpe field trial, demonstrate this medicament to the control efficiency of downy mildew of garpe, effective dose, to the safety of grape and control effect to the double of other diseases of grape.Test method is with reference to " GB/T 17980.122-2004 pesticide field efficacy medicine test criterion (two) the bactericidal agent for preventing and treating downy mildew of garpe test of pesticide effectiveness ".Trial crops is grape, and controlling object is grape downy mildew mildew (Plasmopara viticola).Test kind is huge peak, and the age of tree 10 years, grows fine.Be experimental field level land, fertility is better, experimental field cultivation condition uniformity.
Test medicine and dosage are: 30% pyraclostrobin cyazofamid suspending agent 3000 times, 3500 times, 4000 times, 65% pyraclostrobin phosethyl-Al wetting powder 1000 times, 1500 times, 2000 times, 25% pyraclostrobin missible oil 1500 times, 10% cyazofamid suspending agent 1500 times, 80% phosethyl-Al wetting powder 300 times.Separately establish blank, often process 4 times and repeat, 10, every community tree, Gong40Ge community, random district group arranges.Use sprayer even spraying.The first time dispenser when blade begins to see morbidity, the dispenser 1 time again every 10 days, dispenser 2 times altogether.
Investigation and statistical method:
After second time medicine 7 days, every community random searching 10 then pumping was newly climing, investigates whole blade from top to bottom, by the following stage division record sick number of sheets at different levels and total number of sheets.
Blade stage division:
0 grade: without scab;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for 6% ~ 25% of whole leaf area;
5 grades: lesion area accounts for 26% ~ 50% of whole leaf area
7 grades: lesion area accounts for 51% ~ 75% of whole leaf area;
9 grades: lesion area accounts for more than 75% of whole leaf area.
Disease index and control efficiency computational methods:
Disease index=[∑ (the sick number of sheets × relative level numerical value at different levels)/(investigating the total number of sheets × 9)] × 100
Control efficiency (%)=[(contrast disease index-process disease index)/contrast disease index] × 100
Security survey and the impact on other biological: some skies after the 1st day and medicine after each spray medicine, observe medicament and respectively process grape growth and the impact on other diseases.
Field test results shows, 30% pyraclostrobin cyazofamid suspending agent, 65% pyraclostrobin phosethyl-Al wetting powder has good control efficiency to downy mildew of garpe, dilute 3000 times respectively, 3500 times, 4000 times and 1000 times, 1500 times, 2000 times of sprayings, investigation in 7 days after second time dispenser, sick leaf rate comparison illumination is aobvious to be reduced, preventive effect is respectively 91.28%, 86.89%, 81.19% and 87.32%, 81.85%, 81.10%, obviously be better than 25% pyraclostrobin missible oil 1500 times, 10% cyazofamid suspending agent 1500 times, the preventive effect of 80% phosethyl-Al wetting powder 300 times.
By carrying out biostatistics Epidemiological Analysis (see table 4) to each community preventive effect, significance of difference comparative result shows: consistent with the significance of difference in 0.01 level in 0.05 level, significant difference between 30% pyraclostrobin cyazofamid suspending agent 3000 times, 3500 times, 4000 times, significant difference between 65% pyraclostrobin phosethyl-Al wetting powder 1000 times and 1500 times, 2000 times, between 1500 times, 2000 times, difference is not remarkable.Three variable concentrations all 25% pyraclostrobin missible oil 1500 times of these two kinds of medicaments, 10% cyazofamid suspending agent 1500 times, between 80% phosethyl-Al wetting powder 300 times, difference is extremely remarkable.
Duration of test is observed, and each chemicals treatment does not produce poisoning phenomenon to grape leaf, the tender tip.
Experimental observation finds, 30% pyraclostrobin cyazofamid suspending agent, 65% pyraclostrobin phosethyl-Al wetting powder also have good preventive effect to bitter rot or anthracnose of grape, and 25% pyraclostrobin missible oil preventive effect is general, 10% cyazofamid suspending agent, 80% phosethyl-Al wettable preventive effect are very poor.
After result of the test shows pyraclostrobin and cyazofamid, phosethyl-Al mixture, significantly improve the control efficiency to downy mildew of garpe, also have anthracnose and well doublely control effect, reduce dosage and drug cost, to grape growth safety, it is the favourable agents preventing and treating downy mildew of garpe.
Biological Examples 2: prevent and treat leaf spot of peanut field trial
Leaf spot of peanut is always in increasing the weight of occurrence tendency in recent years, and inventor has carried out example of formulations 2(40% pyraclostrobin cyazofamid water dispersible granules in 2011 at Henan Zhumadian), example of formulations 11(39% pyraclostrobin phosethyl-Al suspending agent) prevent and treat leaf spot of peanut field trial.Test method is with reference to " GB/T 17980.85-2004 pesticide field efficacy medicine test criterion (two) the bactericidal agent for preventing and treating leaf spot of peanut test of pesticide effectiveness ".Trial crops is peanut, and kind is for spending 22, and controlling object is the cercospora brown spot of peanut (Cercospora arachidicola).Be experimental field level land, fertility is better, experimental field cultivation condition uniformity.
Test medicine and dosage are: 40% pyraclostrobin cyazofamid water dispersible granules 12g/667 ㎡, 10g/667 ㎡, 8g/667 ㎡, 39% pyraclostrobin phosethyl-Al suspending agent 50g/667 ㎡, 40g/667 ㎡, 30g/667 ㎡, 25% pyraclostrobin missible oil 30g/667 ㎡, 10% cyazofamid suspending agent 30g/667 ㎡, 80% phosethyl-Al wetting powder 180g/667 ㎡.Separately establish blank, often process 4 times and repeat, every community 20 ㎡, Gong40Ge community, random district group arranges.Use sprayer even spraying, every 667 ㎡ amount of liquid medicine 50kg.The first time dispenser when mid-August, peanut leaf began to see morbidity, the dispenser 1 time again every 14 days, dispenser 2 times altogether.
Investigation and statistical method:
After first time, second time medicine 14 days respectively, every community diagonal 5 sampling, often some investigation 4 strains, the whole blade of every strain investigation stem, total number of sheets investigated in record, the sick number of sheets.By the following stage division record sick number of sheets at different levels and total number of sheets.
Blade stage division:
0 grade: without scab;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for 6% ~ 25% of whole leaf area;
5 grades: lesion area accounts for 26% ~ 50% of whole leaf area
7 grades: lesion area accounts for 51% ~ 75% of whole leaf area;
9 grades: lesion area accounts for more than 75% of whole leaf area.
Disease index and control efficiency computational methods:
Disease index=[∑ (the sick number of sheets × relative level numerical value at different levels)/(investigating the total number of sheets × 9)] × 100
Control efficiency (%)=[(contrast disease index-process disease index)/contrast disease index] × 100
Security survey: some skies after the 1st day and medicine after each spray medicine, observes medicament and respectively processes impact on peanut growth.
Field test results shows: 40% pyraclostrobin cyazofamid water dispersible granules 12g/667 ㎡, 10g/667 ㎡, 8g/667 ㎡, 39% pyraclostrobin phosethyl-Al suspending agent 50g/667 ㎡, 40g/667 ㎡, 30g/667 ㎡, 80.30%, 76.32%, 70.55% respectively to the preventive effect of 14 days after leaf spot of peanut first time medicine, 79.42%, 74.29%, 72.05%, 89.24%, 74.32%, 79.58% is respectively, 88.98%, 87.47%, 78.51% to the preventive effect of 14 days after leaf spot of peanut second time medicine.Obviously be better than 25% pyraclostrobin missible oil 30g/667 ㎡, 10% cyazofamid suspending agent 30g/667 ㎡, 80% phosethyl-Al wetting powder 180g/667 ㎡, to the preventive effect (see table 5) of 14 days after 14 days and second time medicine after leaf spot of peanut first time medicine, shows good quick-acting and lasting effect.
Duration of test is observed, and each chemicals treatment does not produce poisoning phenomenon to peanut.
After result of the test shows pyraclostrobin and cyazofamid, phosethyl-Al mixture, significantly improving the control efficiency to leaf spot of peanut, reduce dosage and drug cost, to peanut growth safety, is the favourable agents preventing and treating leaf spot of peanut.
In sum, composition of the present invention is employing two kinds of active component compound schemes, its activity and bactericidal effect are not the simple superposition of each composition activity, compared with existing unitary agent, except there is significant bactericidal effect, and have significant synergistic effect, and good to crop safety, meet the security requirement of pesticidal preparations.
Claims (2)
1. the bactericidal composition of pyraclostrobin-containing is preventing and treating the application in downy mildew of garpe, described composition active component is 13% pyraclostrobin, 52% phosethyl-Al, 4% calcium lignosulfonate, 3% TERSPERSE 2700,2% Nekal BX, 2%K-12,3% White Carbon black, and fine particle calcium carbonate adds to 100% weight portion.
2. the bactericidal composition of pyraclostrobin-containing is preventing and treating the application in leaf spot of peanut, described composition active component is 13% pyraclostrobin, 26% phosethyl-Al, this dish of 2%TERSPERSE 2020,2%-60#, 2% Tween-60 #, 1%TERSPERSE 2500,0.5% white carbon, 0.3% xanthans, 5% ethylene glycol, 0.5% benzoic acid, 0.5% silicone defoaming agent, 1% epoxychloropropane, and deionized water adds to 100% weight portion.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1258194A (en) * | 1997-05-28 | 2000-06-28 | 巴斯福股份公司 | Fungicidal mixtures |
| CN101647456A (en) * | 2009-06-22 | 2010-02-17 | 陕西标正作物科学有限公司 | Antifungal composite with pyraclostrobin and cymoxanil |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1258194A (en) * | 1997-05-28 | 2000-06-28 | 巴斯福股份公司 | Fungicidal mixtures |
| CN101647456A (en) * | 2009-06-22 | 2010-02-17 | 陕西标正作物科学有限公司 | Antifungal composite with pyraclostrobin and cymoxanil |
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