CN104542585A - Fungicide composition and application thereof - Google Patents
Fungicide composition and application thereof Download PDFInfo
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- CN104542585A CN104542585A CN201410768399.8A CN201410768399A CN104542585A CN 104542585 A CN104542585 A CN 104542585A CN 201410768399 A CN201410768399 A CN 201410768399A CN 104542585 A CN104542585 A CN 104542585A
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Abstract
The invention provides a fungicide composition and application thereof. Active components of the fungicide composition include meptyldinocap and one or at least two of azoxystrobin, trifloxystrobin, fluoxastrobin, dimoxystrobin, picoxystrobin, metominostrobin, orysastrobin, enestroburin, pyraoxystrobin and SYP-1620, wherein the weight ratio of the two components is (1 to 50)-(50 to 1). The invention further provides the application of the fungicide composition in the prevention and control of plant diseases caused by fungi. The fungicide composition has the beneficial effects that compared with the sterilizing effect of a single component, the sterilizing effect is remarkably improved, an excellent synergistic effect is achieved, the consumption of a medicament is reduced, the pesticide cost of farmers is lowered, and meanwhile, the environmental influence is alleviated; meanwhile, the bactericidal spectrum is expanded, and the generation and development of resistance of the single component can be effectively delayed.
Description
Technical field
The present invention relates to disinfectant use in agriculture field, specifically, relate to a kind of with nitre benzene bacterium ester be selected from one or more microbicide compositions being active component and application thereof in Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, ZEN 90160, SSF 126, orysastrobin, Enestroburin, azoles bacterium ester, alkene oxime amine.
Background technology
Nitre benzene bacterium ester belongs to Mitochondria uncoupler; there is the contact bactericide of certain osmotically active; by suppressing the respiration of spore germination and fungi and upsetting the metabolism of fungal cell, provide protective effect, therapeutic action and eradicant action.The chemical name of nitre benzene bacterium ester is 2-(1-methylheptyl)-4,6-dinitrophenyl (2E)-butenoate (2-(1-methylheptyl)-4,6-dinitrophenyl (2E)-2-butenoate), its molecular formula is C18H24N2O6, molecular weight 364.4, structural formula is:
Fluoxastrobin belongs to methoxy acrylic ester compounds; it is a kind of mitochondrial respiratory inhibitor; there is broad spectrum disease prevention and control capability; all have fine activity to nearly all Mycophytes (Ascomycetes, Basidiomycetes, Oomycete and Fungi Imperfecti) disease such as the tens of kinds of diseases such as powdery mildew, rust, glume blight, net blotch, scab, downy mildew, rice blast, and nitre benzene bacterium ester has protection, treats, roots out, permeates, systemic activity.The chemical name of Fluoxastrobin is (E)-[2-[6-(2-cyano-benzene oxygen) pyrimidine-4-yl oxygen] phenyl]-3-methoxy-methyl acrylate, and its molecular formula is C22H17N3O5, molecular weight 403.39, and structural formula is:
Oxime bacterium ester is fluorine bactericide; this compounds derives from natural products strobilurin; it is mitochondrial respiratory inhibitor; namely characteristics such as suppressing mitochondrial breathing by electro transfer between cytochrome b and C1, there is efficient, wide spectrum, protection, treat, root out, permeate, systemic activity, resistance of rainwater washing against, lasting period are long.To Isosorbide-5-Nitrae-demethylation enzyme inhibitor, benzamides, the bacterial strain that dicarboxyl amine and benzimidazole produce resistance is effective, with current existing bactericide without cross resistance.Good activity is all had to nearly all Mycophytes (Ascomycetes, Basidiomycetes, Oomycete and Fungi Imperfecti) disease such as powdery mildew, rust, Ying's rot, net blotch, downy mildew, rice blast etc.Except having special efficacy to powdery mildew, leaf spot, there is good activity to rust, downy mildew, damping off, apple apple scab, sclerotinia rot of colza.To crop safety, because it is at soil, capable of being fast degraded in water, thus environmentally safe.The chemical name of oxime bacterium ester is (2Z)-2-methoxyimino-2-[2-[[1-[3-(trifluoromethyl) phenyl] ethyleneimino] oxygen methyl] phenyl] methyl acetate, and its molecular formula is C
20h
19f
3n
2o
4, molecular weight 408.4, structural formula is:
The mechanism of action of fluoxastrobin is also mitochondrial respiratory inhibitor, namely suppresses mitochondrial breathing by electro transfer between cytochrome b and C1.There is the bactericidal activity of wide spectrum, to nearly all Mycophytes (Ascomycetes, Basidiomycetes, Oomycete and Fungi Imperfecti) disease as the tens of kinds of diseases such as rust, clever blessing disease, net blotch, powdery mildew, downy mildew all have good activity.The chemical name of fluoxastrobin is { 2-[6-(2-chlorophenoxy)-5-FU-4 base oxygen base] phenyl } (5,6-dihydro-Isosorbide-5-Nitrae, 2-dioxazine-3-base) ketone O-methyloxime, molecular formula is C21H16ClFN4O5, molecular weight 458.8, and structural formula is:
The mechanism of action of dimoxystrobin is also mitochondrial respiratory inhibitor, namely suppresses mitochondrial breathing by electro transfer between cytochrome b and C1.Prevent and treat the bacterial strain of 14-demethylation enzyme inhibitor, benzamides, tri carboxylic acid amide class and benzimidazole generation resistance effective.There is protection, treat, root out, permeate, systemic activity.There is the bactericidal activity of wide spectrum, be mainly used in preventing and treating powdery mildew, downy mildew, rice blast, banded sclerotial blight etc.The chemical name of dimoxystrobin is (E)-2-(methoxyimino)-N-methyl-2-[α-(2,5-dimethyl phenoxy) o-tolyl] acetamide, and molecular formula is C
19h
22n
2o
3, molecular weight 326.4, structural formula is:
The mechanism of action of ZEN 90160 is also mitochondrial respiratory inhibitor, namely suppresses mitochondrial breathing by electro transfer between cytochrome b and C1.Prevent and treat the bacterial strain of 14-demethylation enzyme inhibitor, benzamides, tri carboxylic acid amide class and benzimidazole generation resistance effective.ZEN 90160, once be absorbed by blade, will move, flow with current in transportation system in xylem; It also flows and flows in xylem again after absorption enters blade from gas phase in the gas phase of blade surface.There is the bactericidal activity of wide spectrum, for preventing and treating the foliage disease of wheat class as leaf blight, leaf rust, glume blight, brown spot, powdery mildew etc., compared with existing strobilurin series bactericidal agent, there is stronger result for the treatment of to wheat leaf blessing disease, net blotch and moire disease.The chemical name of ZEN 90160 is (E)-3-methoxyl group-2-{2-[6-(trifluoromethyl)-2-pyridine oxygen methyl] phenyl } methyl acrylate, molecular formula is C
18h
16f
3nO
4, molecular weight 367.3, structural formula is:
The mechanism of action of SSF 126 is also mitochondrial respiratory inhibitor, namely suppresses mitochondrial breathing by electro transfer between cytochrome b and C1.Be mainly used in paddy rice control rice blast at present.The chemical name of SSF 126 is (E)-2-methoxyimino-N-methyl-2-(2-phenoxy phenyl) acetamide.Molecular formula is C
16h
16n
2o
3, molecular weight 284.3, structural formula is:
The mechanism of action of orysastrobin is also mitochondrial respiratory inhibitor, namely suppresses mitochondrial breathing by electro transfer between cytochrome b and C1.Be mainly used in paddy rice control rice blast and banded sclerotial blight at present.The chemical name of orysastrobin is (2E)-2-(methoxyimino)-2-{2-[(3E, 5E, 6E)-S-(methoxyimino)-4,6-dimethyl-2,8-dioxa-3,7-dioxa diene-1-in the ninth of the ten Heavenly Stems-3,6-base] phenyl }-N-methyl second phthalein amine, molecular formula is C
18h
25n
5o
5, molecular weight 391.4, structural formula is:
Enestroburin is first methoxy acrylic bactericide of domestic-developed, for the respiration inhibitor of fungal mitochondria, its mechanism of action is by the combination with cytochrome b c1 complex, suppresses mitochondrial electron transmission, thus destroy the synthesis of germ energy, play bactericidal action.Enestroburin belongs to wide, the active high bactericide of methoxy acrylic fungicidal spectrum, has Prevention and Curation effect, has good control efficiency to by mastigomycetes, the plurality of plant diseases that causes in conjunction with bacterium, sac fungi, basidiomycetes and imperfect fungus.The chemical name of Enestroburin is α-[2-[[[[4-(4-chlorphenyl)-Ding-3-alkene-2-base] imido grpup] oxygen base] methyl] phenyl]-'beta '-methoxy acrylic acid methyl esters, and molecular formula is C
22h
27clNO
4, molecular weight 399.9, structural formula is:
Azoles bacterium ester is novel methoxy acrylic ester compounds, is mitochondrial respiratory inhibitor, the main activity suppressing compound III.High, the disease-resistant spectrum of this series bactericidal agent bactericidal activity is wide and good with Environmental compatibility, mechanism of action is unique, it is to ascomycetes, as Sclerotinia sclerotiorum, fusarium graminearum etc.), basidiomycetes, as wheat rust germ, Rhizoctonia solani Kuhn), oomycetes is as phytophthora infestans) and Fungi Imperfecti, as cucumber anthracnose, Alternaria brassicae etc.) the mycelial growth of more than 20 kind of encountered pathogenic fungi all there is inhibit activities.The chemical name of azoles bacterium ester is (E)-2-(2-((3-(4-chlorphenyl)-1-methyl isophthalic acid H-pyrazoles-5-base oxygen base) methyl) phenyl)-3-methoxy-methyl acrylate, molecular formula is C22H21ClN2O4, molecular weight 412.9, structural formula is:
Alkene oxime amine is the methoxy acrylic efficient agricultural bactericide that Shenyang Chemical Engineering Inst is lead compound recent development with natural antibiotics strobilurin, its mechanism of action suppresses mitochondrial respiration by stoping the electrical conductivity between cytochrome b and c1, effectively can prevent and treat the pathogen series other bactericide being produced to anti-row.Alkene oxime amine fungicidal spectrum is wide, bactericidal activity is high, have protection and therapeutic action; with environmental organism, there is good compatibility; to by mastigomycetes, the plurality of plant diseases that causes in conjunction with bacterium, sac fungi, basidiomycetes and imperfect fungus, there is good control efficiency; and can crop yield be improved; improve product quality, have excellent control efficiency to the crop powdery mildews such as melon, wheat, cucumber downy mildew, cladosporium leaf and fruit mould of tomato, tomato late blight, wheat rust, alternaria leaf spot of apple.The chemical name of alkene oxime amine is N-methyl-2-[((((1-methyl-3-(2,6-dichlorophenyl)-2-acrylic) imido grpup) oxygen base) methyl) phenyl]-2-methoxyimino acetic amide, molecular formula is C21H21Cl2N3O4, molecular weight 434.6, structural formula is:
Summary of the invention
The object of the present invention is to provide the microbicide compositions of a kind of fungicidal spectrum extensively, to control of plant disease Be very effective.
Another object of the present invention is to the application that described microbicide compositions is provided.
The present inventor finds through large quantity research, nitre benzene bacterium ester and appointment bactericide (Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, ZEN 90160, SSF 126, orysastrobin, Enestroburin, azoles bacterium ester, alkene oxime amine): composite use within the specific limits, there is obviously synergistic effect, the control efficiency of fungus-caused plant disease is significantly improved, and can fungicidal spectrum be expanded.In order to realize object of the present invention, in an aspect, the invention provides a kind of microbicide compositions, its active component be nitre benzene bacterium ester and be selected from Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, ZEN 90160, SSF 126, orysastrobin, Enestroburin, azoles bacterium ester, alkene oxime amine one or more, the weight ratio of the two is 1:50 ~ 50:1; Be preferably 1:20 ~ 20:1; More preferably 1:10 ~ 10:1; More preferably 1:9 ~ 9:1.
In other respects, the present invention also provides the application of above-mentioned microbicide compositions, and it is for preventing and treating crop pest.
In other aspects, the present invention also provides a kind of method of preventing and treating crop pest, and it comprises the step to the above-mentioned bactericidal composition of crop applying.
Microbicide compositions of the present invention at least has the following advantages:
1, nitre benzene bacterium ester shows fabulous synergistic effect with appointment bactericide (Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, ZEN 90160, SSF 126, orysastrobin, Enestroburin, azoles bacterium ester, alkene oxime amine) is composite in certain ratio range, bactericidal effect is significantly improved compared with single dose, thus reduce the usage amount of bactericide, while minimizing peasant drug cost, reduce the impact on environment;
2, expand fungicidal spectrum, make a kind of bactericide can prevent and treat plurality of plant diseases simultaneously, provide a kind of means of prevention easily and effectively;
3, said composition is the compounded combination of mitochondrial respiratory inhibitor series bactericidal agent and oxidative phosphorylation uncoupler series bactericidal agent, and different action characteristics makes to produce extremely strong mutual supplement with each other's advantages between two kinds of medicaments, effectively can delay generation and the development of single dose resistance.
Embodiment
In an aspect, the invention provides a kind of microbicide compositions, its active component be nitre benzene bacterium ester and be selected from Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, ZEN 90160, SSF 126, orysastrobin, Enestroburin, azoles bacterium ester, alkene oxime amine one or more, the weight ratio of the two is 1:50 ~ 50:1; Be preferably 1:20 ~ 20:1; More preferably 1:10 ~ 10:1; More preferably 1:9 ~ 9:1.
The amount of application when content of active component depends on that often kind of component is used alone in microbicide compositions, also the degree of ratio between two kinds of components and synergistic effect is depended on, in microbicide compositions of the present invention, the weight percentage of its active component is 0.5 ~ 90%; In addition, described microbicide compositions also can comprise surfactant and carrier.
Described surfactant comprises one or more in emulsifier, dispersant or wetting agent;
Wherein, described emulsifier is selected from sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene fatty acid ester, polyoxyethylene fatty acid diester, polyoxyethylene aliphatic alcohol ether, Polyoxyethylene fatty ammonia, ethoxy castor oil, fatty acid polyethylene glycol ester, alkylphenol polyoxyethylene, Crodaret, polyoxyethylene alkyl phenyl ether, polyoxyethylene dialkyl phenyl organic ether, polyoxyethylene alkyl phenyl ether/formaline condensates, polyoxyethylene/polyoxypropylene block polymer ether, alkyl polyoxyethylene/polyoxypropylene block polymer ether, alkyl phenyl polyethylene oxides/polyoxypropylene block polymer ether, polyoxyethylene diphenyl ether, polyoxyethylene benzyl phenyl ether, polyoxyethylene styryl phenyl ether, Polyoxyethylene Ether siloxanes, ester type siloxanes, one or more in lecithin and polyoxyethylene aliphatic alcohol ether,
Described dispersant is be selected from one or more in lignosulfonates, Sodium Polyacrylate, naphthalene sulfonate, alkylnaphthalene sulfonate, alkyl amide taurate, polycarboxylate, the sodium salt of alkyl naphthalene sulfonic acid condensation polymer and alkyl naphthalene sulfonic acid yuban;
One or more for being selected from lauryl sodium sulfate, Teepol, neopelex, in fatty alcohol polyglycol ether sulphate, alkylnaphthalene sulfonate and anion wetting agent of described wetting agent.
Described carrier is selected from one or more in white carbon, kaolin, diatomite, precipitated calcium carbonate, soluble starch, urea, amine fertilizer, glucose, maltose, sucrose, Anhydrous potassium carbonate, natrium carbonicum calcinatum, Carbon Dioxide hydrogen potassium and anhydrous sodium bicarbonate; As selected the mixture Anhydrous potassium carbonate of urea and amine fertilizer, the mixture of anhydrous sodium bicarbonate and Carbon Dioxide hydrogen potassium or the mixture of natrium carbonicum calcinatum and anhydrous sodium bicarbonate are as carrier.
In addition, described bactericidal composition also comprises one or more in solvent, cosolvent, antifreezing agent, thickener, protectant, capsule material, defoamer, disintegrant and binding agent.
Wherein, described solvent is selected from one or more in vegetable oil, mineral oil, solvent naphtha and water; By its dissolubility, water or oil can be divided into.
Wherein, described vegetable oil is epoxidized soybean oil, soybean oil, peanut oil, rapeseed oil, castor oil, corn oil, pine nut wet goods; Described mineral oil is liquid wax, machine oil, kerosene, lubrication wet goods; Described solvent naphtha can select No. 100, solvent naphtha, No. 150 or No. 200 etc.
Described cosolvent is be selected from one or more in 1-METHYLPYRROLIDONE, oxolane, dimethyl sulfoxide (DMSO), spectrasolv DMDA, DMF, tetrahydrofurfuryl alcohol, tributyl phosphate, Isosorbide-5-Nitrae-dioxane and cyclohexanone.
Described antifreezing agent is ethylene glycol, propane diols, glycerine or urea.
Described thickener is be selected from one or more in xanthans, polyvinyl alcohol, POLYPROPYLENE GLYCOL, polyethylene glycol, white carbon, sodium alginate, Magnesiumaluminumsilicate, carboxymethyl cellulose, carboxy-propyl cellulose sodium and organobentonite.
Described protectant is be selected from one or more in polyvinyl alcohol, polyethylene glycol etc.
Described capsule material is polyurethane, polyureas or Lauxite etc.
Described defoamer is organosiloxane, tributyl phosphate or silicone.
Described disintegrant for being selected from sodium phosphate trimer, calgon, Metallic stearates, cellulose powder, dextrin, the copolymer of methacrylate, one or more in polyvinylpyrrolidone etc.
Described binding agent is for being selected from carboxymethyl cellulose and salt thereof, dextrin, water soluble starch, sucrose, polyvinylpyrrolidone, gum Arabic, polyvinyl alcohol, polyvinyl acetate, Sodium Polyacrylate, polyethylene glycol, natural phospholipid, xanthan gum, one or more in guar gum etc.
The formulation of microbicide compositions of the present invention can be the conventional various formulations in this area, as emulsifiable concentrate, aqueous emulsion, aqueous suspension agent, oil-suspending agent, microcapsule suspending agent, wetting powder, water-dispersible granules, soluble powder, soluble granula, the microemulsion that above-mentioned active component and various auxiliary agent, surfactant and carrier can be mixed with, above-mentioned preparation can be prepared by the method that this area is general.
The composition of wherein said soluble granula, soluble powder, water dispersible granules and wetting powder is active component 0.5 ~ 90%, wetting agent 1 ~ 30%, dispersant 1 ~ 30%, carrier complement to 100%.
Described aqueous emulsion and microemulsion consist of active component 0.5 ~ 90%, emulsifier 1 ~ 30%, antifreezing agent 1 ~ 10%, water complements to 100%;
Described emulsifiable concentrate consist of active component 0.5 ~ 90%, cosolvent 1 ~ 30%, emulsifier 1 ~ 30%, solvent complement to 100%;
Described suspension emulsion consists of active component 0.5 ~ 90%, solvent 0.5 ~ 10%, surfactant 1 ~ 30%, antifreezing agent 1 ~ 10%, thickener 0.1 ~ 5%, water complement to 100%;
Described oil-suspending agent consist of active component 0.5 ~ 90%, surfactant 1 ~ 30%, thickener 0.1 ~ 5%, oil phase complement to 100%;
Described microcapsule suspending agent and microcapsule suspending agent-suspending agent preferably consist of active component 0.5 ~ 90%, solvent 1 ~ 20.0%, protectant 0.1 ~ 5.0%; capsule material 1 ~ 30%, dispersant 1 ~ 30%, wetting agent 1 ~ 30%, antifreezing agent 1 ~ 10%, thickener 0.1 ~ 5%, water complement to 100%.
Preferably, the preparation method of above-mentioned formulation is as follows:
The preparation of soluble granula or water dispersible granules: active component and carrier, wetting agent and binding agent etc. are carried out co-grinding, spray-dried or fluidized drying and get final product; Or add water kneading after co-grinding, adds in comminutor and carry out granulation, and then drying, screening, to obtain final product.
The preparation of soluble powder or wetting powder: by the fully mixing such as each active component, various wetting agent, dispersant and carrier, through ultra-fine pulverizer disintegrating and get final product.
The preparation of aqueous emulsion: by each active component, emulsifier mixing, make its uniformly oil phase; By water, antifreezing agent mixing, it is made to become homogeneous aqueous phase.Under high velocity agitation, aqueous phase joined oil phase or oil phase is joined aqueous phase, forming the aqueous emulsion of favorable dispersibility.
The preparation of microemulsion: by each active component, emulsifier mixing, make its uniformly oil phase; By water, antifreezing agent mixing, make it become homogeneous aqueous phase, under high velocity agitation, aqueous phase is joined oil phase or oil phase is joined aqueous phase, form microemulsion.
The preparation of emulsifiable concentrate: each active component, solvent, cosolvent and emulsifier are mixed, makes its property uniformly oil phase, to obtain final product.
The preparation of suspension emulsion: take water as medium, active component B, dispersant, thickener and antifreezing agent etc. are added in sand milling still, carries out porphyrize, make aqueous suspension agent; Active component A, solvent, emulsifier are fully mixed into oil phase; Oil phase is added aqueous suspension agent, forms suspension emulsion.
The preparation of oil-suspending agent: take oil as medium, each active component, dispersant, thickener and antifreezing agent etc. are added in sand milling still, carries out porphyrize, make oil-suspending agent.
Prepared by microcapsule suspending agent: active component, solvent and capsule material are mixed; Mixture adds emulsification in the aqueous phase containing protectant and defoamer, until particle reaches required size, adds alkaline aqueous solution and completes to polymerisation, then adds thickener and dispersant obtains stable microcapsule suspending agent.Microbicide compositions of the present invention can provide with finished product preparation form, and namely in composition, each material mixes; Also can provide with independent dosage form, mix voluntarily in bucket or tank before using, its concentrate usually and water be mixed to get the concentration of required active substance.
Microcapsule suspending agent-suspending agent preparation: by active components A or the mixing of B, solvent and capsule material; Be mixed into required size, add alkaline aqueous solution and complete to polymerisation, then add thickener and dispersant obtains stable microcapsule suspending agent.With oil or water for medium, active component A or B, dispersant, thickener and antifreezing agent etc. are added in sand milling still, carry out porphyrize, make oil-suspending agent or aqueous suspension agent.By microcapsule suspending agent and suspending agent mixing, water supplies surplus, makes microcapsule suspending agent-suspending agent.
Microbicide compositions of the present invention can provide with finished product preparation form, and namely in composition, each material mixes; Also can provide with independent dosage form, mix voluntarily in bucket or tank before using, its concentrate usually and water be mixed to get the concentration of required active substance.
In other respects, the present invention also provides the application of above-mentioned microbicide compositions, and it is for preventing and treating crop pest.In addition, the present invention also provides a kind of method of preventing and treating crop pest, and it comprises the step to the above-mentioned bactericidal composition of crop applying.
Microbicide compositions of the present invention can be used for preventing and treating fungus-caused plant disease.
Microbicide compositions of the present invention is applicable to various crop, the vegetables such as such as cucumber, pumpkin, custard squash, tomato, onion, shallot, beet, capsicum; The field crop such as wheat class, cereal, paddy rice; The economic crops such as mung bean, tobacco, flax; The fruit such as strawberry, grape, citrus; Flowers and other crops such as tree peony, chrysanthemum, Chinese rose, rose, rose.
Microbicide compositions of the present invention has obvious synergistic effect compared with single dose, all has good control efficiency for plant diseases such as powdery mildew, downy mildew, late blight, peronospora tabacina, blight dis-ease, white rot, early blight, rice blast, banded sclerotial blight, balck shank, damping off, black spot, spot defoliation, canker, root rot, mottled rot disease, gray mold, ring spot, scab, net blotch, anthracnose, fusarium wilt, rust, glume blight.
Composition of the present invention also can have sterilization with other, the compound of desinsection or herbicidal performance uses, also can be used in combination with nematocide, protective agent, growth regulator, nutrient for plants or soil conditioner.
The using method of microbicide compositions of the present invention is simple, before plant disease is sprouted or after sprouting, place to crop and plant growth is used according to a conventional method, and as mixed soil, spraying, injection, cast etc., its amount of application is determined according to weather conditions or crop condition.Microbicide compositions of the present invention, its bactericidal effect is usually relevant with the content of compound individualized in composition, also with extraneous factor as weather is relevant, but can climatic influences be slowed down by the suitable formulation of use.
Following examples are used for the present invention is described, but are not used for limiting the scope of the invention.As nothing specializes, the content in embodiment is percetage by weight, and medicament used is commercial.
Embodiment 1 is containing the missible oil of 10% active component
Nitre benzene bacterium ester 60g, fluoxastrobin 40g, alkylphenol polyoxyethylene 20g, fatty alcohol-polyoxyethylene ether 80g, No. 150, solvent naphtha are mended to 1kg, is fully mixed into homogeneous oil phase, namely obtain the emulsifiable concentrate containing 10% active component.
Embodiment 2 is containing the missible oil of 10% active component
Nitre benzene bacterium ester 50g, alkene oxime amine 50g, tetrahydrofurfuryl alcohol 100.0g, fatty alcohol-polyoxyethylene ether 50.0g, castor oil polyoxyethylene ether 50.0g, No. 150, solvent naphtha are mended to 1kg, is fully mixed into homogeneous oil phase, namely obtain the emulsifiable concentrate containing 20% active component.
Embodiment 3 is containing the emulsifiable concentrate of 30% active component
Nitre benzene bacterium ester 100g, Enestroburin 200g, 1-METHYLPYRROLIDONE 100g, sorbitan fatty acid ester 100g, polyoxyethylene fatty acid ester 50g, No. 150, solvent naphtha are mended to 1kg, fully be mixed into homogeneous oil phase, namely obtain the emulsifiable concentrate containing 30% active component.
Embodiment 4 is containing the emulsifiable concentrate of 60% active component
Nitre benzene bacterium ester 400g, Fluoxastrobin 200g, dimethyl formamide 10g, dodecyl sodium sulfonate calcium 50g, fatty alcohol-polyoxyethylene ether 50g, No. 100, solvent naphtha are mended to 1kg, is fully mixed into homogeneous oil phase, namely obtain the emulsifiable concentrate containing 60% active component.
Embodiment 5 is containing the microemulsion of 10% active component
Nitre benzene bacterium ester 5g, oxime bacterium ester 95g, solvent naphtha No. 150 200g, styryl phenyl APEO 300g, water complement to 1kg.
Above-mentioned nitre benzene bacterium ester, oxime bacterium ester, No. 150, solvent naphtha, styryl phenyl APEO are mixed into homogeneous oil phase; Under agitation, water is joined in oil phase, form the microemulsion containing 10% active component.
Embodiment 6 is containing the microemulsion of 30% active component
Nitre benzene bacterium ester 150g, azoles bacterium ester 150g, 1-METHYLPYRROLIDONE 100g, secondary octyl phenol polyethenoxy ether 100g, ethoxy castor oil 150g, ethylene glycol 50g, water complement to 1kg.
Above-mentioned nitre benzene bacterium ester, azoles bacterium ester, 1-METHYLPYRROLIDONE, secondary octyl phenol polyethenoxy ether, ethoxy castor oil are mixed into homogeneous oil phase; Under agitation, water is joined in oil phase, form the microemulsion containing 30% active component.
Embodiment 7 is containing the microemulsion of 45% active component
Nitre benzene bacterium ester 50g, fluoxastrobin 400g, solvent naphtha No. 150 200g, decyl alcohol polyoxyethylene ether sulfosuccinic acid sodium 100g, ethoxy castor oil APEO 150g, water complement to 1kg.
Above-mentioned nitre benzene bacterium ester, fluoxastrobin, No. 150, solvent naphtha, decyl alcohol polyoxyethylene ether sulfosuccinic acid sodium, ethoxy castor oil APEO are mixed into homogeneous oil phase; Under speed stirs, water is joined in oil phase, form the microemulsion containing 45% active component.
Embodiment 8 is containing the microemulsion of 60% active component
Nitre benzene bacterium ester 200g, SSF 126 400g, alkyl phenol polyoxyethylene polyoxypropylene ether 180g, sorbitan fatty acid ester APEO 20g, 1-METHYLPYRROLIDONE 100g, water complement to 1kg.
Above-mentioned nitre benzene bacterium ester, SSF 126, alkyl phenol polyoxyethylene polyoxypropylene ether, sorbitan fatty acid ester APEO, 1-METHYLPYRROLIDONE are mixed into homogeneous oil phase; Under agitation, aqueous phase is joined in oil phase, form the microemulsion containing 60% active component.
Embodiment 9 is containing the aqueous emulsion of 10% active component
Nitre benzene bacterium ester 5g, dimoxystrobin 95g, solvent naphtha No. 150 100g, polyoxyethylene styryl phenyl ether 50g, POLYPROPYLENE GLYCOL 80g, diatomite 30g, water are complemented to 1kg.
Above-mentioned nitre benzene bacterium ester, dimoxystrobin, No. 150, solvent naphtha, polyoxyethylene styryl phenyl ether are mixed into homogeneous oil phase; POLYPROPYLENE GLYCOL, diatomite and water are mixed into homogeneous aqueous phase; Under high velocity agitation, aqueous phase is joined in oil phase, form the aqueous emulsion containing 10% active component of favorable dispersibility
Embodiment 10 is containing the aqueous emulsion of 30% active component
Nitre benzene bacterium ester 150g, ZEN 90160 150g, 1-METHYLPYRROLIDONE 100g, alkylphenol polyoxyethylene 30g, fatty alcohol-polyoxyethylene ether 20g, ethylene glycol 50g, sodium alginate 10g, water complement to 1kg.
Above-mentioned nitre benzene bacterium ester, ZEN 90160,1-METHYLPYRROLIDONE, alkylphenol polyoxyethylene, fatty alcohol-polyoxyethylene ether are mixed into homogeneous oil phase; Ethylene glycol, sodium alginate and water are mixed into homogeneous aqueous phase; Under high velocity agitation, oil phase is joined in aqueous phase, form the aqueous emulsion containing 30% active component of favorable dispersibility
Embodiment 11 is containing the aqueous emulsion of 45% active component
Nitre benzene bacterium ester 150g, alkene oxime amine 300g, tributyl phosphate 150g, castor oil 10g, polyoxyethylene aliphatic alcohol ether 50g, ethoxy castor oil 50g, xanthans 3g, urea 50g, water complement to 1kg.
Above-mentioned nitre benzene bacterium ester, alkene oxime amine, castor oil, tributyl phosphate 150g, polyoxyethylene aliphatic alcohol ether, ethoxy castor oil are mixed into homogeneous oil phase; Xanthans, urea and water are mixed into homogeneous aqueous phase; Under high velocity agitation, oil phase is joined in aqueous phase, form the aqueous emulsion containing 45% active component of favorable dispersibility
Embodiment 12 is containing the aqueous emulsion of 60% active component
Nitre benzene bacterium ester 400g, azoles bacterium ester 200g, alkyl polyoxyethylene/polyoxypropylene block polymer ether 30g, polyoxyethylene sorbitan fatty acid ester 40g, tetrahydrofurfuryl alcohol 150g, carboxymethyl cellulose 10g, polyethylene glycol 50g, water complement to 1kg.
Above-mentioned nitre benzene bacterium ester, azoles bacterium ester, alkyl polyoxyethylene/polyoxypropylene block polymer ether, polyoxyethylene sorbitan fatty acid ester, 1-METHYLPYRROLIDONE are mixed into homogeneous oil phase; Propylene glycol alginate, polyethylene glycol and water are mixed into homogeneous aqueous phase; Under high velocity agitation, aqueous phase is joined in oil phase, form the aqueous emulsion containing 60% active component of favorable dispersibility
Embodiment 13 is containing the suspension emulsion of 10% active component
Nitre benzene bacterium ester 10g, alkene oxime amine 90g, ethoxy castor oil 10g, polyoxyethylene aliphatic alcohol ether 30g, diatomite 30g, propane diols 60g, water complement to 1kg.
Take water as medium, above-mentioned alkene oxime amine, ethoxy castor oil, fatty alcohol-polyoxyethylene ether, diatomite, propane diols are added porphyrize in sand milling still, namely obtain containing alkene oxime amine aqueous suspension agent; Nitre benzene bacterium ester is joined alkene oxime amine aqueous suspension agent, namely obtains the suspension emulsion containing 10% active component.
Embodiment 14 is containing the suspension emulsion of 20% active component
Nitre benzene bacterium ester 190g, Fluoxastrobin 10g, solvent naphtha No. 150 50g, polyoxyethylene sorbitan fatty acid ester 50g, fatty alcohol-polyoxyethylene ether 50g, white carbon 40g, propane diols 80g, water complement to 1kg.
Take water as medium, above-mentioned Fluoxastrobin, polyoxyethylene sorbitan fatty acid ester, fatty alcohol-polyoxyethylene ether, white carbon, propane diols are added porphyrize in sand milling still, namely obtains Fluoxastrobin aqueous suspension agent; Nitre benzene bacterium ester, No. 150, solvent naphtha, polyoxyethylene sorbitan fatty acid ester, fatty alcohol-polyoxyethylene ether are fully mixed into oil phase; Oil phase joins Fluoxastrobin aqueous suspension agent, namely obtains the suspension emulsion containing 20% active component.
Embodiment 15 is containing the suspension emulsion of 50% active component
Formula: nitre benzene bacterium ester 300g, orysastrobin 200g, tetrahydrofurfuryl alcohol 100g, polyoxyethylene alkyl phenyl ether/formaline condensates 30g, ethoxy castor oil, 20g, propane diols 30g, lagoriolite 10g, water complements to 1kg.
Take water as medium, above-mentioned nitre benzene bacterium ester, orysastrobin, polyoxyethylene alkyl phenyl ether/formaline condensates, urea, lagoriolite are added porphyrize in sand milling still, namely obtain containing orysastrobin aqueous suspension agent; Nitre benzene bacterium ester, tetrahydrofurfuryl alcohol are mixed into oil phase; Oil phase is joined orysastrobin aqueous suspension agent, namely obtain the suspension emulsion containing 50% active component.
Embodiment 16 is containing the suspension emulsion of 60% active component
Formula: nitre benzene bacterium ester 100g, dimoxystrobin 500g, solvent naphtha No. 100 50g, sodium methylene bis-naphthalene sulfonate (dispersing agent NNO) 30g, lauryl sodium sulfate 10g, fatty alcohol-polyoxyethylene ether 50g, urea 50g, xanthans 1g, water complement to 1kg.
Take water as medium, above-mentioned dimoxystrobin, dispersing agent NNO, lauryl sodium sulfate, urea, xanthans are added porphyrize in sand milling still, namely obtain containing Fluoxastrobin aqueous suspension agent; Nitre benzene bacterium ester, fatty alcohol-polyoxyethylene ether, No. 100, solvent naphtha are mixed into oil phase; Oil phase is joined Fluoxastrobin aqueous suspension agent, namely obtain the suspension emulsion containing 60% active component.
Embodiment 17 is containing the oil-suspending agent of 13.5% active component
Nitre benzene bacterium ester 85g, Fluoxastrobin 50g, polyoxyethylene styryl phenyl ether 100g, polyoxyethylene aliphatic alcohol ether 50g, diatomite 10g, corn oil subsidy are to 1kg.
Take corn oil as medium, above-mentioned nitre benzene bacterium ester, Fluoxastrobin, polyoxyethylene styryl phenyl ether, polyoxyethylene aliphatic alcohol ether, diatomite are added porphyrize in sand milling still, namely obtain the oil-suspending agent containing 13.5% active component.
Embodiment 18 is containing the oil-suspending agent of 45% active component
Nitre benzene bacterium ester 100g, fluoxastrobin 350g, ethoxy castor oil 50g, Polyoxyethylene fatty ammonia 50g, white carbon 30g, soybean oil subsidy are to 1kg.
Take soybean oil as medium, above-mentioned nitre benzene bacterium ester, fluoxastrobin, ethoxy castor oil, Polyoxyethylene fatty ammonia, silica are added porphyrize in sand milling still, namely obtain the oil-suspending agent containing 45% active component.
Embodiment 19 is containing the oil-suspending agent of 30% active component
Nitre benzene bacterium ester 30g, Enestroburin 270g, polyoxyethylene aliphatic alcohol ether 40g, polyoxyethylene sorbitan fatty acid ester 50g, organobentonite 30g, castor-oil plant oil subsidy are to 1kg.
Take castor oil as medium, above-mentioned nitre benzene bacterium ester, Enestroburin, polyoxyethylene aliphatic alcohol ether, polyoxyethylene sorbitan fatty acid ester, organobentonite are added porphyrize in sand milling still, namely obtain the oil-suspending agent containing 30% active component.
Embodiment 20 is containing the oil-suspending agent of 15% active component
Nitre benzene bacterium ester 60g, orysastrobin 90g, ethoxy castor oil 50g, alkylphenol polyoxyethylene 50g, organobentonite 40g, soybean oil subsidy are to 1kg.
Take soybean oil as medium, above-mentioned nitre benzene bacterium ester, orysastrobin, ethoxy castor oil, alkylphenol polyoxyethylene, organobentonite are added porphyrize in sand milling still, namely obtain the oil-suspending agent containing 15% active component.
Embodiment 21 is containing the oil-suspending agent of 4% active component
Formula: nitre benzene bacterium ester 7g, oxime bacterium ester 33g, alkylphenol polyoxyethylene 40g, alkyl phenyl polyethylene oxides/polyoxypropylene block polymer ether 30g, clay 30g, liquid wax are mended to 1kg.
With liquid wax for medium, above-mentioned nitre benzene bacterium ester, oxime bacterium ester, alkylphenol polyoxyethylene, alkyl phenyl polyethylene oxides/polyoxypropylene block polymer ether, clay are added porphyrize in sand milling still, namely obtain the oil-suspending agent containing 4% active component.
Embodiment 22 is containing the oil-suspending agent of 4% active component
Formula: nitre benzene bacterium ester 33g, Enestroburin 7g, polyoxyethylene fatty acid ester 30g, alkylphenol polyoxyethylene 10.0g, organobentonite 30g, paraffin oil subsidy are to 1kg.
Take paraffin oil as medium, by above-mentioned nitre benzene bacterium ester, Enestroburin, polyoxyethylene fatty acid ester, alkylphenol polyoxyethylene 10.0g, organobentonite adds porphyrize in sand milling still, namely obtains the oil-suspending agent containing 4% active component.
Embodiment 23 is containing the wetting powder of 0.6% active component
Nitre benzene bacterium ester 1g, SSF 126 5g, sodium lignin sulfonate 30g, sodium methylene bis-naphthalene sulfonate (dispersant NNO) 20g, starch 50g, kaolin complement to 1kg
Said components is fully mixed, after ultra-fine pulverizer disintegrating, namely obtains the wetting powder containing 0.06% active component.
Embodiment 24 is containing the wetting powder of 10% active component
Nitre benzene bacterium ester 10g, Fluoxastrobin 90g, lauryl sodium sulfate 10g, sodium methylene bis-naphthalene sulfonate (dispersant NNO) 30g, white carbon complement to 1kg.
Said components is fully mixed, after ultra-fine pulverizer disintegrating, namely obtains the wetting powder containing 10% active component.
Embodiment 25 is containing the wetting powder of 25% active component
Nitre benzene bacterium ester 190g, ZEN 90160 60g, sodium lignin sulfonate 20g, lauryl sodium sulfate 50g, diatomite are complemented to 1kg.
Said components is fully mixed, after ultra-fine pulverizer disintegrating, namely obtains the wetting powder containing 25% active component.
Embodiment 26 is containing the wetting powder of 80% active component
Nitre benzene bacterium ester 600g, alkene oxime amine 200g, sodium lignin sulfonate 30g, white carbon complement to 1kg.
Said components is fully mixed, after ultra-fine pulverizer disintegrating, namely obtains the wetting powder containing 80% active component.
Embodiment 27 is containing the wetting powder of 80% active component
Nitre benzene bacterium ester 200g, fluoxastrobin 600g, alkylnaphthalene sulfonate 50g, lauryl sodium sulfate 50g, diatomite complements to 1kg.
Said components is fully mixed, after ultra-fine pulverizer disintegrating, namely obtains the wetting powder containing 80% active component.
Embodiment 28 is containing the water-dispersible granules of 1.0% active component
Nitre benzene bacterium ester 6g, SSF 126 4g, HPMC 50g, lignin sulfonic acid 50g, soluble starch complement to 1kg.
By above-mentioned nitre benzene bacterium ester, SSF 126, HPMC, sodium lignin sulfonate, soluble starch co-grinding, make the water-dispersible granules containing 1.0% active component through mist projection granulating.
Embodiment 29 is containing the water-dispersible granules of 50% active component
Nitre benzene bacterium ester 100g, azoles bacterium ester 400g, sodium lignin sulfonate 150g, calcium lignosulfonate 50g, white carbon 100g, kaolin complement to 1kg.
By above-mentioned nitre benzene bacterium ester, azoles bacterium ester, alkylphenol polyoxyethylene succinate monoester sulfonate, calcium lignosulfonate, white carbon co-grinding, make the water-dispersible granules containing 50% active component through fluidized granulating.
Embodiment 30 is containing the water-dispersible granules of 80% active component
Nitre benzene bacterium ester 125g, ZEN 90160 675g, alkylnaphthalene sulfonate 20g, lauryl sodium sulfate 40g, white carbon complements to 1kg.
By above-mentioned nitre benzene bacterium ester, ZEN 90160, alkylnaphthalene sulfonate, lauryl sodium sulfate 40g, white carbon co-grinding; add water after mediating; add in the comminutor that diameter 1.0mm screen cloth is housed and carry out granulation; and then drying, 10 ~ 30 mesh sieves divide, make the water-dispersible granules containing 80% active component.
Embodiment 31 is containing the microcapsule suspending agent of 10% active component
Nitre benzene bacterium ester 2g, Fluoxastrobin 8g, solvent naphtha No. 150 21g, polyureas 3g, polyvinyl alcohol 1.0g, organosiloxane 0.05g, xanthans 0.15g, sodium lignin sulfonate 3.0g, water are mended to 100g.
By nitre benzene bacterium ester, Fluoxastrobin, mix with the mixture of No. 150, solvent naphtha and polyureas; Then this mixture is added emulsification in the mixture be made up of polyvinyl alcohol, silicone defoaming agent and water.Be uniformly mixed until polymerisation completes; Add xanthans and sodium lignin sulfonate again, obtain stable microcapsule suspending agent.This capsule diameter intermediate value is 5 ~ 8 microns.
Embodiment 32 is containing the micro-capsule dry suspending agent of 25% active component
Nitre benzene bacterium ester 10g, Fluoxastrobin 15g, solvent naphtha No. 150 5g, polyureas 5g, polyvinyl alcohol 1.0g, xanthans 0.15g, sodium lignin sulfonate 3.0g, fatty alcohol-polyoxyethylene ether 3g, water is mended to 100g.
The mixture of nitre benzene bacterium ester with No. 150, solvent naphtha and polyureas is mixed; Then this mixture is added emulsification in the mixture be made up of polyvinyl alcohol, silicone defoaming agent and water.Be uniformly mixed until polymerisation completes; Fluoxastrobin, fatty alcohol-polyoxyethylene ether, water are added porphyrize in sand milling still, obtains Fluoxastrobin suspending agent; By microcapsule suspending agent and suspending agent mixing, then xanthans and sodium lignin sulfonate will be added, obtain stable microcapsule suspending agent-suspending agent.
The present invention is further illustrated below by way of sterilization effect experiment.Percentage in embodiment is ratio of weight and number, but the present invention is not limited thereto;
Experimental technique: adopt indoor biological determination of activity, by composite to the determination of activity of target fungus indoor biological to active components A (A is: Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, alkene oxime amine, azoles bacterium ester) and nitre benzene bacterium ester.Test medicine is originated: Beijing Nutrichem Biotechnology Co., Ltd.'s preparation research room.Determine that after the effective inhibition concentration scope of each medicament, each medicament establishes 7 dosage process respectively according to active constituent content through preliminary experiment, if clear water contrast, carry out with reference to " farm-chemical indoor determination test rule bactericide ".
Active components A (A is: Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, alkene oxime amine, azoles bacterium ester) and nitre benzene bacterium ester and the composite indoor biological determination of activity to powdery mildew of cucumber thereof, this test adopts pot-culture method to measure.Cucumber seeds is seeded in the basin of 13 × 13 specifications, and 8 cucumber sowed by every basin, each process 2 basin.Cultivate in greenhouse, when cucumber seedling grew to for 3 leaf phase, every basin stays the well-grown cucumber seedling of 5 strain for subsequent use.
By preparation liquid pneumatic energy nozzle (shower nozzle model be Spraying System company produce Air Atom shower nozzle) 10 milliliters of even sprayings of liquid on 10 strain cucumber plants, naturally dry.Test is established and is not made blank containing the process of medicament.
24h after chemicals treatment, with the cucumber white powder spore suspension spray inoculation prepared, greenhouse compartment (the relative moisture 95% of temp. controllable humidity is moved to after inoculation, temperature 20 DEG C) cultivate 24h under dark condition, then at 20 DEG C ~ 25 DEG C, 12h alternation of light and darkness, relative moisture 90% cultivates 7d.
When the sick leaf rate of blank reaches more than 50%, classification investigation manages incidence everywhere, adopts following stage division:
0 grade: without scab;
1 grade: lesion area accounts for less than 5% of one-piece blade area;
3 grades: lesion area accounts for 5% ~ 15% of one-piece blade area;
5 grades: lesion area accounts for 15% ~ 25% of one-piece blade area;
7 grades: lesion area accounts for 25% ~ 50% of one-piece blade area;
9 grades: lesion area accounts for more than 50% of one-piece blade area;
Data statistic analysis
Computational methods: according to Disease investigation data, calculate disease index and the control efficiency of each process.
Adopt regression analysis to analyze test data, inhibiting rate is converted into probability value, with each drug concentration logarithm value for abscissa, calculates EC by linear regression analysis method
50and EC
90value, the virulence size of more each treatment agent.
Calculate two medicament different ratio synergies ratio (SR) according to Wadley method, SR<0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, and SR>1.5 is synergistic effect simultaneously.Computing formula is as follows:
Wherein: a, b are respectively active components A (A is: Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, ZEN 90160, alkene oxime amine, Enestroburin, SSF 126, orysastrobin, azoles bacterium ester) and nitre benzene bacterium ester proportion in the composition;
A is: Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, ZEN 90160, alkene oxime amine, Enestroburin, SSF 126, orysastrobin, azoles bacterium ester;
B is nitre benzene bacterium ester
It is as shown in the table for result of the test:
Table 1 Fluoxastrobin, nitre benzene bacterium ester and the composite indoor biological determination of activity result to powdery mildew of cucumber thereof
As shown in Table 1, Fluoxastrobin and nitre benzene bacterium ester mixed synergies within the scope of 50:1 ~ 1:50 ideal, within the scope of 9:1 ~ 1:9, mixed synergies is particularly outstanding.
Table 2 alkene oxime amine, nitre benzene bacterium ester and the composite indoor biological determination of activity result to powdery mildew of cucumber thereof
As shown in Table 2, alkene oxime amine and nitre benzene bacterium ester mixed synergies within the scope of 50:1 ~ 1:50 ideal, within the scope of 9:1 ~ 1:9, mixed synergies is particularly outstanding.
Active components A (A is: Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, alkene oxime amine, azoles bacterium ester) and nitre benzene bacterium ester and the composite indoor biological determination of activity to cucumber downy mildew thereof, this test adopts plate blade method to measure.
On the basis of preliminary experiment, with the aqueous solution containing 0.5% Tween 80 for solvent, dilution preparation, respectively containing the liquid process of following agents concentration, is respectively: 200mg/L, 100mg/L, 50mg/L, 25mg/L, 12.5mg/L, 6.25,3.125mg/L.
Never cucumber downy mildew leaf was gathered with using the cucumber cultivation of bactericide, moisturizing is in culture dish, be placed in 19 DEG C, relative moisture 90%, cultivate under 12h alternation of light and darkness condition, after incidence of leaf producing sporangium new in a large number, with 0.5% tween sterile water, spore is eluted, be mixed with the sporangia suspension that concentration is 105 sporangium/mL, for inoculation test.
Get from cucumber the 4 to 6 healthy leaves slightly from the top down, the cucumber leaves card punch of health being made diameter is that the leaf dish of 1.5cm is placed in the liquid of variable concentrations and soaks 0.5h, then leaf dish is taken out, remove liquid unnecessary on leaf dish, leaf back upward, adopt sessile drop method inoculating spores suspension 10 μ l in leaf dish central authorities, first dark infects 24h, then postvaccinal blade is placed in 19 DEG C, cultivates under the condition of 12h alternation of light and darkness.Each process 40 leaf dishes.
During contrast morbidity more than 50%, adopt the onset area on area reckoning method measurement leaf dish, calculate inhibiting rate compared with control treatment, calculate control efficiency by formula (1), represent with percentage (%), calculate and retain 2 significant digits.
P＝(D
0—D
1)*100/D
0(1)
In formula:
P-control efficiency;
D
0-blank lesion diameter;
D
1-chemicals treatment lesion diameter.
Adopt regression analysis to analyze test data, control efficiency is converted into probability value, with each drug concentration logarithm value for abscissa, calculates EC by linear regression analysis method
50and EC
90value, the virulence size of more each treatment agent.
Evaluate NC51 and NC401 according to Wadley method and use synergy type to cucumber downy mildew with, coefficient of synergism (SR) <0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR>1.5 is synergistic effect.Coefficient of synergism (SR) calculates by formula (2), formula (3):
Wherein: a, b are respectively active components A (A is: Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, ZEN 90160, alkene oxime amine, Enestroburin, SSF 126, orysastrobin, azoles bacterium ester) and nitre benzene bacterium ester proportion in the composition;
A is: Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, ZEN 90160, alkene oxime amine, Enestroburin, SSF 126, orysastrobin, azoles bacterium ester;
B is nitre benzene bacterium ester
It is as shown in the table for result of the test:
Table 3 Fluoxastrobin, nitre benzene bacterium ester and the composite indoor biological determination of activity result to cucumber downy mildew thereof
As shown in Table 3, Fluoxastrobin and nitre benzene bacterium ester mixed synergies within the scope of 50:1 ~ 1:50 ideal, within the scope of 9:1 ~ 1:9, mixed synergies is particularly outstanding.
Table 4 fluoxastrobin, nitre benzene bacterium ester and the composite indoor biological determination of activity result to cucumber downy mildew thereof
As shown in Table 4, fluoxastrobin and nitre benzene bacterium ester mixed synergies within the scope of 50:1 ~ 1:50 ideal, within the scope of 9:1 ~ 1:9, mixed synergies is particularly outstanding.
Active components A (A is: Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, alkene oxime amine, azoles bacterium ester) and nitre benzene bacterium ester and the composite indoor biological determination of activity to fruit white rot of grape and watermelon grafting thereof, adopt mycelial growth rate method to measure.
According to preliminary experiment situation, the composite toxic medium being mixed into variable concentrations of each ratio, test is established not containing the blank of chemicals treatment, each process repetition four times.By cultured grape white rot stem or Didymella bryoniae, use the sterilizing card punch of diameter 6mm under aseptic technique, select the bacterium colony that growing way is consistent, bacterium cake is cut from colony edge, with inoculator, pure culture biscuits involvng inoculation is dull and stereotyped central in pastille, mycelia, towards upper, covers ware lid, is placed in 25 DEG C of incubator dark culturing.
According to the growing state investigation pathogen mycelial growth situation of bacterium colony in blank culture dish, after the bacterium colony in blank fully grows, measure the colony diameter of each process with right-angled intersection method, adopt formulae discovery bacterium colony below to increase diameter, get its mean value.
Bacterium colony increases diameter=colony diameter-bacterium cake diameter (1)
The following method of measurement result calculates, and the bacterium colony increasing diameter and chemicals treatment with blank bacterium colony increases diameter and calculates each chemicals treatment to the mycelial growth inhibition rate of rice sheath blight disease pathogen (see formula below).
Adopt regression analysis to analyze test data, mycelial growth inhibition rate is converted into probability value, with each drug concentration logarithm value for abscissa, calculates EC by linear regression analysis method
50and EC
90value, the virulence size of more each treatment agent.
Evaluate NC51 and NC401 according to Wadley method and use synergy type to cucumber downy mildew with, coefficient of synergism (SR) <0.5 is antagonism, 0.5≤SR≤1.5 are summation action, and SR>1.5 is synergistic effect.Coefficient of synergism (SR) calculates by formula (2), formula (3):
Wherein: a, b are respectively active components A (A is: Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, ZEN 90160, alkene oxime amine, Enestroburin, SSF 126, orysastrobin, azoles bacterium ester) and nitre benzene bacterium ester proportion in the composition;
A is: Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, ZEN 90160, alkene oxime amine, Enestroburin, SSF 126, orysastrobin, azoles bacterium ester;
B is nitre benzene bacterium ester
It is as shown in the table for result of the test:
Table 5 Fluoxastrobin, nitre benzene bacterium ester and the composite indoor biological determination of activity result to fruit white rot of grape thereof
As shown in Table 5, Fluoxastrobin and nitre benzene bacterium ester mixed synergies within the scope of 50:1 ~ 1:50 ideal, within the scope of 9:1 ~ 1:9, mixed synergies is particularly outstanding.
Table 6 dimoxystrobin, nitre benzene bacterium ester and the composite indoor biological determination of activity result to fruit white rot of grape thereof
As shown in Table 6, dimoxystrobin and nitre benzene bacterium ester mixed synergies within the scope of 50:1 ~ 1:50 ideal, within the scope of 9:1 ~ 1:9, mixed synergies is particularly outstanding.
Table 7 Fluoxastrobin, nitre benzene bacterium ester and the composite indoor biological determination of activity result to watermelon grafting thereof
As shown in Table 7, azoles bacterium ester and nitre benzene bacterium ester mixed synergies within the scope of 50:1 ~ 1:50 ideal, within the scope of 9:1 ~ 1:9, mixed synergies is particularly outstanding.
Table 8 oxime bacterium ester, nitre benzene bacterium ester and the composite indoor biological determination of activity result to watermelon grafting thereof
As shown in Table 8, oxime bacterium ester and nitre benzene bacterium ester mixed synergies within the scope of 50:1 ~ 1:50 ideal, within the scope of 9:1 ~ 1:9, mixed synergies is particularly outstanding.
Wheat powdery mildew experimental technique: active components A (A is: ZEN 90160, Enestroburin) and nitre benzene bacterium ester and the composite indoor biological determination of activity to wheat powdery mildew thereof, this test adopts pot-culture method to measure.Wheat seed is seeded in the basin of 13 × 13 specifications, and 8 cucumber sowed by every basin, each process 2 basin.Cultivate in greenhouse, when wheat seedling grew to for 3 leaf phase, every basin stays the well-grown wheat seedling of 5 strain for subsequent use.
By preparation liquid pneumatic energy nozzle (shower nozzle model be Spraying System company produce Air Atom shower nozzle) 10 milliliters of even sprayings of liquid on 10 strain cucumber plants, naturally dry.Test is established and is not made blank containing the process of medicament.
24h after chemicals treatment, with the white wheat oidium suspension spray inoculation prepared, greenhouse compartment (the relative moisture 95% of temp. controllable humidity is moved to after inoculation, temperature 20 DEG C) cultivate 24h under dark condition, then at 20 DEG C ~ 25 DEG C, 12h alternation of light and darkness, relative moisture 90% cultivates 7d.
When the sick leaf rate of blank reaches more than 50%, classification investigation manages incidence everywhere, adopts following stage division:
0 grade: without scab;
1 grade: lesion area accounts for less than 5% of one-piece blade area;
3 grades: lesion area accounts for 5% ~ 15% of one-piece blade area;
5 grades: lesion area accounts for 15% ~ 25% of one-piece blade area;
7 grades: lesion area accounts for 25% ~ 50% of one-piece blade area;
9 grades: lesion area accounts for more than 50% of one-piece blade area;
Data statistic analysis
Computational methods: according to Disease investigation data, calculate disease index and the control efficiency of each process.
Adopt regression analysis to analyze test data, inhibiting rate is converted into probability value, with each drug concentration logarithm value for abscissa, calculates EC by linear regression analysis method
50and EC
90value, the virulence size of more each treatment agent.
Calculate two medicament different ratio synergies ratio (SR) according to Wadley method, SR<0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, and SR>1.5 is synergistic effect simultaneously.
Computing formula is as follows:
Wherein: a, b are respectively active components A (A is: ZEN 90160, Enestroburin) and nitre benzene bacterium ester proportion in the composition;
A is: ZEN 90160, Enestroburin;
B is nitre benzene bacterium ester
It is as shown in the table for result of the test:
Table 9 ZEN 90160, nitre benzene bacterium ester and the composite indoor biological determination of activity result to wheat powdery mildew thereof measure
As shown in Table 9, ZEN 90160 and nitre benzene bacterium ester mixed synergies within the scope of 50:1 ~ 1:50 ideal, within the scope of 9:1 ~ 1:9, mixed synergies is particularly outstanding.
Table 10 Enestroburin, nitre benzene bacterium ester and the composite indoor biological determination of activity result to wheat powdery mildew thereof measure
As shown in Table 10, Enestroburin and nitre benzene bacterium ester mixed synergies within the scope of 50:1 ~ 1:50 ideal, within the scope of 9:1 ~ 1:9, mixed synergies is particularly outstanding.
Rice blast experimental technique: active components A (A is: SSF 126, orysastrobin) and nitre benzene bacterium ester and the composite indoor biological determination of activity to rice blast thereof, this test adopts pot-culture method to measure.Wheat seed is seeded in the basin of 13 × 13 specifications, and 8 paddy rice sowed by every basin, each process 2 basin.Cultivate in greenhouse, when wheat seedling grew to for 3 leaf phase, every basin stays the well-grown wheat seedling of 5 strain for subsequent use.
By preparation liquid pneumatic energy nozzle (shower nozzle model be Spraying System company produce Air Atom shower nozzle) 10 milliliters of even sprayings of liquid on 10 strain cucumber plants, naturally dry.Test is established and is not made blank containing the process of medicament.
24h after chemicals treatment, with the white wheat oidium suspension spray inoculation prepared, greenhouse compartment (the relative moisture 95% of temp. controllable humidity is moved to after inoculation, temperature 20 DEG C) cultivate 24h under dark condition, then at 20 DEG C ~ 25 DEG C, 12h alternation of light and darkness, relative moisture 90% cultivates 7d.
When the sick leaf rate of blank reaches more than 50%, classification investigation manages incidence everywhere, adopts following stage division:
0 grade: without scab;
1 grade: lesion area accounts for less than 5% of one-piece blade area;
3 grades: lesion area accounts for 5% ~ 15% of one-piece blade area;
5 grades: lesion area accounts for 15% ~ 25% of one-piece blade area;
7 grades: lesion area accounts for 25% ~ 50% of one-piece blade area;
9 grades: lesion area accounts for more than 50% of one-piece blade area;
Data statistic analysis
Computational methods: according to Disease investigation data, calculate disease index and the control efficiency of each process.
Adopt regression analysis to analyze test data, inhibiting rate is converted into probability value, with each drug concentration logarithm value for abscissa, calculates EC by linear regression analysis method
50and EC
90value, the virulence size of more each treatment agent.
Calculate two medicament different ratio synergies ratio (SR) according to Wadley method, SR<0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, and SR>1.5 is synergistic effect simultaneously.Computing formula is as follows:
Wherein: a, b are respectively active components A (A is: SSF 126, orysastrobin) and nitre benzene bacterium ester proportion in the composition;
A is: SSF 126, orysastrobin;
B is nitre benzene bacterium ester
It is as shown in the table for result of the test:
Table 11 SSF 126, nitre benzene bacterium ester and the composite indoor biological determination of activity result to rice blast thereof measure
As shown in Table 11, SSF 126 and nitre benzene bacterium ester mixed synergies within the scope of 50:1 ~ 1:50 ideal, within the scope of 9:1 ~ 1:9, mixed synergies is particularly outstanding.
Table 12 orysastrobin, nitre benzene bacterium ester and the composite indoor biological determination of activity result to rice blast thereof measure
As shown in Table 12, orysastrobin and nitre benzene bacterium ester mixed synergies within the scope of 50:1 ~ 1:50 ideal, within the scope of 9:1 ~ 1:9, mixed synergies is particularly outstanding.
Application examples: field control effectiveness test
Test medicine is researched and developed by Beijing Nutrichem Biotechnology Co., Ltd., is provided, contrast medicament: 36% nitre benzene bacterium emulsifiable concentrate (commercially available), 250g/l Fluoxastrobin suspending agent (commercially available), 500g/l oxime bacterium ester suspending agent (self-control), 480g/l fluoxastrobin suspending agent (self-control), 12.5% dimoxystrobin missible oil (self-control), 22.5% ZEN 90160 suspending agent (commercially available), 30% SSF 126 wetting powder (self-control), 30% orysastrobin aqueous emulsion (self-control), 25% Enestroburin missible oil (commercially available), 20% azoles bacterium ester suspending agent (self-control), 5% alkene oxime amine missible oil (commercially available),
Application examples 1 nitre benzene bacterium ester and active components A prevent and treat the powdery mildew of cucumber test of pesticide effectiveness
This test arrangement is in suburb, Linyi, and the investigation powdery mildew of cucumber state of an illness before medicine, in first time at state of an illness initial stage spray pesticide, dispenser in every 7 days once, is total to dispenser 2 times.7 days, 14 days, 30 days disease indexs after second time dispenser also calculate preventive effect.Experimental result is as following table:
Table 13 nitre benzene bacterium ester and active components A and compositely prevent and treat the powdery mildew of cucumber test of pesticide effectiveness
(commercially available), (self-control), (self-control), (self-control), (commercially available), (self-control), (self-control), (commercially available), (self-control),
Application examples 2 nitre benzene bacterium ester and active components A prevent and treat the cucumber downy mildew test of pesticide effectiveness
This test arrangement is in Xingtai City suburb, Hebei province, and the investigation cucumber downy mildew state of an illness before medicine, in first time at state of an illness initial stage spray pesticide, dispenser in every 7 days once, is total to dispenser 2 times.7 days, 14 days, 30 days disease indexs after second time dispenser also calculate preventive effect.Experimental result is as following table:
Table 14 nitre benzene bacterium ester and active components A and the composite control cucumber downy mildew test of pesticide effectiveness thereof
Application examples 3 nitre benzene bacterium ester and active components A prevent and treat the grape powdery mildew test of pesticide effectiveness;
This test arrangement is in suburb, Changli County, Qinhuangdao, Hebei province, and the investigation grape powdery mildew state of an illness before medicine, in first time at state of an illness initial stage spray pesticide, dispenser in every 7 days once, is total to dispenser 2 times.7 days, 14 days, 30 days disease indexs after second time dispenser also calculate preventive effect.Experimental result is as following table:
Table 15 nitre benzene bacterium ester and active components A and the composite control grape powdery mildew test of pesticide effectiveness thereof
Application examples 4 nitre benzene bacterium ester and active components A prevent and treat the climing withered test of pesticide effectiveness of watermelon;
This test arrangement is in suburb, Daxing district, Beijing, China, and the investigation watermelon grafting state of an illness before medicine, in first time at state of an illness initial stage spray pesticide, dispenser in every 7 days once, is total to dispenser 2 times.7 days, 14 days, 30 days disease indexs after second time dispenser also calculate preventive effect.Experimental result is as following table:
Table 16 nitre benzene bacterium ester and active components A and the composite control watermelon grafting test of pesticide effectiveness thereof
Application examples 5 nitre benzene bacterium ester and active components A prevent and treat the wheat powdery mildew test of pesticide effectiveness;
This test arrangement is in suburb, Jining City in Shandong Province, and the investigation wheat powdery mildew state of an illness before medicine, in first time at state of an illness initial stage spray pesticide, dispenser in every 7 days once, is total to dispenser 2 times.7 days, 14 days, 30 days disease indexs after second time dispenser also calculate preventive effect.Experimental result is as following table:
Table 17 nitre benzene bacterium ester and active components A and the composite control wheat powdery mildew test of pesticide effectiveness thereof
Application examples 6 nitre benzene bacterium ester and active components A prevent and treat the rice blast test of pesticide effectiveness;
This test arrangement is in suburb, Anqing, Anhui Province, and the investigation rice blast state of an illness before medicine, in first time at state of an illness initial stage spray pesticide, dispenser in every 7 days once, is total to dispenser 2 times.7 days, 14 days, 30 days disease indexs after second time dispenser also calculate preventive effect.Experimental result is as following table:
Table 18 nitre benzene bacterium ester and active components A and the composite control rice blast test of pesticide effectiveness thereof
Also it should be noted that, can implement and not obvious run counter to the prerequisite of purport of the present invention under, in this manual as a certain technical scheme component part described by arbitrary technical characteristic or the combination of technical characteristic equally also go for other technical scheme; Further, can implement and not obvious run counter to the prerequisite of purport of the present invention under, as different technologies scheme component part described by technical characteristic between also can combine in any way, form other technical scheme.The technical scheme that the present invention obtains by combining under being also contained in above-mentioned situation, and these technical schemes are equivalent to record in this manual.
Below describe the present invention through the specific embodiment and the embodiment, but it will be understood by those skilled in the art that these are not intended to limit scope of the present invention, scope of the present invention should be determined by claims.
Industrial applicibility
Microbicide compositions of the present invention can be widely used in control of plant disease.
Claims (15)
1. the bactericidal composition containing nitre benzene bacterium ester, it is characterized in that containing active components A and active component B, the weight ratio of active components A and active component B is 1:50 ~ 50:1, described active components A be selected from Fluoxastrobin, oxime bacterium ester, fluoxastrobin, dimoxystrobin, ZEN 90160, SSF 126, orysastrobin, Enestroburin, azoles bacterium ester, alkene oxime amine one or more, active component B is nitre benzene bacterium ester.
2. the bactericidal composition containing nitre benzene bacterium ester according to claim 1, wherein, the weight ratio of described active components A and active component B is 1:30 ~ 30:1.
3. the bactericidal composition containing nitre benzene bacterium ester according to claim 1, wherein, the weight ratio of described active components A and active component B is 1:20 ~ 20:1.
4. the bactericidal composition containing nitre benzene bacterium ester according to claim 1, wherein, the weight ratio of described active components A and active component B is 1:9 ~ 9:1.
5. the bactericidal composition containing nitre benzene bacterium ester according to claim 1, wherein, described active components A is Fluoxastrobin.
6. the bactericidal composition containing nitre benzene bacterium ester according to claim 1, is characterized in that, the weight percentage that active components A described in microbicide compositions and active component B add up to is 0.5 ~ 90%; Described bactericidal composition also comprises surfactant and carrier.
7. the microbicide compositions containing nitre benzene bacterium ester according to claim 6, it is characterized in that, described surfactant comprises one or more in emulsifier, dispersant or wetting agent, wherein, described emulsifier is for being selected from sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene fatty acid ester, polyoxyethylene fatty acid diester, polyoxyethylene aliphatic alcohol ether, Polyoxyethylene fatty ammonia, ethoxy castor oil, fatty acid polyethylene glycol ester, alkylphenol polyoxyethylene, Crodaret, polyoxyethylene alkyl phenyl ether, polyoxyethylene dialkyl phenyl organic ether, polyoxyethylene alkyl phenyl ether/formaline condensates, polyoxyethylene/polyoxypropylene block polymer ether, alkyl polyoxyethylene/polyoxypropylene block polymer ether, alkyl phenyl polyethylene oxides/polyoxypropylene block polymer ether, polyoxyethylene diphenyl ether, polyoxyethylene benzyl phenyl ether, polyoxyethylene styryl phenyl ether, Polyoxyethylene Ether siloxanes, ester type siloxanes, one or more in lecithin and polyoxyethylene aliphatic alcohol ether,
Described dispersant is be selected from one or more in lignosulfonates, Sodium Polyacrylate, naphthalene sulfonate, alkylnaphthalene sulfonate, alkyl amide taurate, polycarboxylate, the sodium salt of alkyl naphthalene sulfonic acid condensation polymer and alkyl naphthalene sulfonic acid yuban;
One or more for being selected from lauryl sodium sulfate, Teepol, neopelex, in fatty alcohol polyglycol ether sulphate, alkylnaphthalene sulfonate and anion wetting agent of described wetting agent.
8. the bactericidal composition containing nitre benzene bacterium ester according to claim 6, is characterized in that, described bactericidal composition also comprise in solvent, cosolvent, antifreezing agent, thickener, protectant, capsule material, defoamer, disintegrant and binding agent one or more.
9. the bactericidal composition containing nitre benzene bacterium ester according to claim 6, it is characterized in that, described carrier is selected from one or more in white carbon, kaolin, diatomite, precipitated calcium carbonate, soluble starch, urea, amine fertilizer, glucose, maltose, sucrose, Anhydrous potassium carbonate, natrium carbonicum calcinatum, Carbon Dioxide hydrogen potassium and anhydrous sodium bicarbonate.
10. the bactericidal composition containing nitre benzene bacterium ester according to claim 6, it is characterized in that, the formulation of described microbicide compositions is emulsifiable concentrate, aqueous emulsion, suspension emulsion, oil-suspending agent, microcapsule suspending agent, micro-capsule dry suspending agent, wetting powder, water-dispersible granules, soluble powder, soluble granula or microemulsion.
11. bactericidal compositions containing nitre benzene bacterium ester according to claim 6, it is characterized in that, the composition of described soluble granula, soluble powder, water dispersible granules and wetting powder is active component 0.5 ~ 90%, wetting agent 1 ~ 30%, dispersant 1 ~ 30%, carrier complement to 100%.
Described aqueous emulsion and microemulsion consist of active component 0.5 ~ 90%, emulsifier 1 ~ 30%, antifreezing agent 1 ~ 10%, water complements to 100%;
Described emulsifiable concentrate consist of active component 0.5 ~ 90%, cosolvent 1 ~ 30%, emulsifier 1 ~ 30%, solvent complement to 100%;
Described suspension emulsion consists of active component 0.5 ~ 90%, solvent 0.5 ~ 10%, surfactant 1 ~ 30%, antifreezing agent 1 ~ 10%, thickener 0.1 ~ 5%, water complement to 100%;
Described oil-suspending agent consist of active component 0.5 ~ 90%, surfactant 1 ~ 30%, thickener 0.1 ~ 5%, oil phase complement to 100%;
Described microcapsule suspending agent and microcapsule suspending agent-suspending agent preferably consist of active component 0.5 ~ 90%, solvent 1 ~ 20.0%, protectant 0.1 ~ 5.0%; capsule material 1 ~ 30%, dispersant 1 ~ 30%, wetting agent 1 ~ 30%, antifreezing agent 1 ~ 10%, thickener 0.1 ~ 5%, water complement to 100%.
12. according to any one of claim 1 to 11 containing the bactericidal composition of nitre benzene bacterium ester for preventing and treating the application of crop pest.
13. application according to claim 12, wherein, described disease comprises powdery mildew, downy mildew, late blight, blight dis-ease, white rot, peronospora tabacina, early blight, rice blast, banded sclerotial blight, balck shank, damping off, black spot, spot defoliation, canker, root rot, mottled rot disease, gray mold, ring spot, scab, net blotch, anthracnose, fusarium wilt, rust, glume blight etc.
14. 1 kinds of methods of preventing and treating crop pest, it comprises the step to the bactericidal composition containing nitre benzene bacterium ester of crop applying according to any one of claim 1 to 11.
15. methods of preventing and treating crop pest according to claim 14, wherein, described disease comprises powdery mildew, downy mildew, late blight, blight dis-ease, white rot, peronospora tabacina, early blight, rice blast, banded sclerotial blight, balck shank, damping off, black spot, spot defoliation, canker, root rot, mottled rot disease, gray mold, ring spot, scab, net blotch, anthracnose, fusarium wilt, rust, glume blight.
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| CN105165828A (en) * | 2015-10-28 | 2015-12-23 | 陕西上格之路生物科学有限公司 | Bactericidal composition containing metrafenone and meptyldinocap |
| CN105613512A (en) * | 2016-03-31 | 2016-06-01 | 广东中迅农科股份有限公司 | Sterilization composition containing isopyrazam and meptyldinocap |
| CN105831117A (en) * | 2016-04-22 | 2016-08-10 | 广东中迅农科股份有限公司 | Sterilization composition containing meptyldinocap and 2-[3-(2,5-dimethyl-phenoxymethyl)-phenyl]-3-methoxy-acrylic acid methyl ester |
| CN112823628A (en) * | 2019-11-21 | 2021-05-21 | 沈阳中化农药化工研发有限公司 | Seed treatment agent containing active component of alkene oxime amine and application thereof |
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| CN112823628A (en) * | 2019-11-21 | 2021-05-21 | 沈阳中化农药化工研发有限公司 | Seed treatment agent containing active component of alkene oxime amine and application thereof |
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