CN106818769A - Bactericidal composition and its application - Google Patents

Bactericidal composition and its application Download PDF

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Publication number
CN106818769A
CN106818769A CN201710043527.6A CN201710043527A CN106818769A CN 106818769 A CN106818769 A CN 106818769A CN 201710043527 A CN201710043527 A CN 201710043527A CN 106818769 A CN106818769 A CN 106818769A
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shenqinmycin
component
bactericidal composition
preparation
fludioxonil
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CN106818769B (en
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古越
李翔
陈熙
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Sichuan Lier Crop Science Co Ltd
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Sichuan Lier Crop Science Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of bactericidal composition and its application, it is related to technical field of pesticide combination.The active ingredient of bactericidal composition of the present invention contains shenqinmycin A and remaining bactericide B, and wherein remaining bactericide B is selected from the phosethyl-Al of cyazofamid, the hymexazo of oxazole series bactericidal agent, the fludioxonil of pyrroles's series bactericidal agent or the organophosphorus fungicide of sulphaguanidine azoles fungicide.A is 1 with B weight ratio：100~100：1.Effective constituents A has obvious synergistic effect with B compoundings, can effectively reduce the amount of application of each single dose.Preferably act on expanding fungicidal spectrum and delaying the plant resistance to the action of a drug to have, moreover it is possible to extend the lasting period, with obvious promotional value.Said composition allows the auxiliary element for using to be processed into any formulation in can adding other agricultural chemicals.The bactericidal composition can effectively prevent and treat banded sclerotial blight, rice blast, false smut, rust, powdery mildew, root rot, sclerotiniose, smut, droop etc..

Description

Bactericidal composition and its application
Technical field
The present invention relates to a kind of bactericidal composition and its application, particularly relate to a kind of containing active ingredient shenqinmycin and work The bactericidal composition of property bactericide and its application, belong to technical field of pesticide combination.
Background technology
In agricultural production, crops can healthy growth, in addition to being influenceed by worm, crop smothering, the preventing and treating to disease is also very heavy Will.Reinforcement and sustainable development implementation with environmental protection ideas, efficient, low toxicity, high activity, low-residual are sent out as agricultural chemicals The inexorable trend of exhibition.However, even efficient germicide, long-term exclusive use, also easily makes disease develop immunity to drugs, cause to use The problems such as dose is increased, preventive effect is reduced, the lasting period shortens, is unfavorable for environment sustainable development.Therefore, how Scientific Usage of Drugs, drop The consumption of low chemical pesticide, improves drug effect, as the problem that plant protection art is badly in need of solving.
Cyazofamid (CyazofaMid) goes out also known as match seat, chloro- 2- cyano group-N, N- dimethyl -5- p-methylphenyl the imidazoles -1- of 4- Sulfonamide, cyanogen azoles flusulfamide, section are good, belong to sulphaguanidine azoles fungicide.Cyazofamid is mitochondrial respiratory inhibitor, to oomycetes institute Having growth phase has effect, and with other bactericide without cross tolerance, with certain interior suction and therapeutic activity, the lasting period is long, resistance to Rain drop erosion, to crop, the mankind, Environmental security, but it is long-term it is single use, germ can be made to develop immunity to drugs it unavoidably.
Hymexazo (hymexazol), also known as hydroxyisoxazole, hymexazol, molecular formula is C4H5NO2, chemical name：3- hydroxyls- 5- methyl-isoxazoles.Hymexazo is systemic fungicide, while being again a kind of soil sterilants.Iron, aluminium in the medicine and soil Ions binding, suppresses the germination of spore.Hymexazol can be moved by the absorption of the root of plant and in root system, be produced in plant intracellular metabolite Two kinds of glucosides, the effect of physiologically active is improved to crop, so as to promote plant strain growth, point evil of root, the increase of root hair and The activity raising of root.Hymexazo is that broad-spectrum germicide has preventing and treating knot higher to the plant disease that various disease funguses cause Really, but it is long-term it is single use, germ can be made to develop immunity to drugs it unavoidably.
Fludioxonil (fludioxonil), also known as fluorine fludioxonil, coughs up bacterium ester, 4- (2,2- bis- fluoro- 1,3- benzos dioxy -4- Base) pyrroles -3- nitriles, the bacterium that do not fall dislike, it is suitable happy when, fludioxonil is new pyrrole class non-internal-absorting wide-spectrum bactericide, its mechanism of action It is the transferase relevant by suppressing thalline glucose phosphorylated, the growth of fungal mycelium can be suppressed, causes germ dead.It is right The microbial disease of the cause of diseases such as Alternaria, Ascochyta, aspergillus, Fusarium, Helminthosporium, Rhizoctonia and Penicillium With protection and therapeutic action well, for preventing and treating the rotten Fusariumsp of snow, wheat black rot, rhizoctonia solani etc..
Phosethyl-Al (phosethyl-Al), also known as Yimeiling, the spirit of epidemic disease frost, aliette, net mould epidemic disease, Methylpartricin Sodium Laurylsulfate, mould Spirit, three second aluminum phosphates, fosetyl aluminium, be a kind of organic phosphates efficiently, wide spectrum, absorbability low toxicity bactericide, with treatment and protect Effect.It can be with upper and lower Bidirectional Conduction in plant.The aqueous solubility is good, and interior sorptivety permeability is strong, and the lasting period is more long, uses Safety.
Shenqinmycin, such as shown in (I), shenqinmycin is secreted through biological culture by pseudomonas fluorescens M18 to its structural formula A kind of antibiotic, while have wide spectrum suppress phytopathogen and promote Effects on Plant Growth dual-use function bactericide, With wide spectrum, it is efficient the characteristics of.
Frequently using containing single-activity component bactericide, causes most germs to generate the resistance to the action of a drug, and preventive effect is not It is preferable.Therefore, it is badly in need of the sterilised products that a kind of novel product substitutes single-activity component, increases the resistance to the action of a drug that drug effect delays disease Risk, reduces economic loss.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of component rationally, and significantly, bactericidal effect is good, medication for synergistic effect Low cost, is not likely to produce the resistance to the action of a drug, the bactericidal composition to crop safety and its application.
In order to solve the above technical problems, present invention offer technical scheme is as follows：
Bactericidal composition of the invention, its active ingredient is shenqinmycin A and active bactericide B, shenqinmycin A and activity The weight ratio of bactericide B is 1:100~100:1, preferably weight ratio is 1：50~50：1, more preferably weight ratio is 1：25~ 25：1.
In bactericidal composition of the invention, the active bactericide can be：The cyazofamid, Evil of sulphaguanidine azoles fungicide It is any one in phosethyl-Al of hymexazo, the fludioxonil of pyrroles's series bactericidal agent or organophosphorus fungicide of azoles fungicide etc. Kind.
Pesticidal preparations auxiliary element can also be contained in bactericidal composition of the present invention, be adapted to agriculturally use to be made Formulation.The pesticidal preparations auxiliary element is the one kind in solvent, emulsifying agent, dispersant, stabilizer, antifreezing agent or wetting agent Or it is several.
Bactericidal composition of the invention can be prepared into any one formulation of suitable agricultural use with known method, than Preferable formulation is dry suspending agent (DF), wettable powder (WP), water dispersible granules (WDG), suspending agent (SC), seed treatment Or suspension seed-coating agent etc..
A wettable powders (WP), be can be dispersed in water solid powder preparation.Wettable powder of the present invention except Outside in active compound plus such as white carbon, precipitated calcium carbonate, potter's clay, diatomite, attapulgite diluent or inert substance, also from Subtype or nonionic surface active agent (wetting agent, dispersant), wetting agent is for example, alkylbenzenesulfonate (DBS-Na), alkyl Naphthalene sulfonate (pull open powder), alkylphenol-polyethenoxy base ether sulfate, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulfate, Aliphatic alcohol sulfate, fatty alcohol ethylene oxide adduct sulfonate, alkyl amide taurate, aliphatic alcohol polyethenoxy base ether (JFC), content 0.1~30%；Dispersant such as naphthalene sulfonate, naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), wood Quality and its derivative sulfonate (M-9, POLYFON etc.), polyoxyethylene poly-oxygen propylene aether block copolymer, alkyl phenol polyoxy second Alkene ether formaldehyde condensation products sulfate (SOPA), PVA, CMC content 0.1~30%；Also containing penetrating agent JFC, azone, thiophene ketone, One or more material among penetrant t, content 0.3~30%；Stabilizer, foam inhibitor, anticaking agent etc. can also be contained Other auxiliary agents.
B water dispersible granules (WDG), are that can be comparatively fast disintegrated in water, disperse to form the granule of higher suspension dispersion.By The auxiliary agent and carrier etc. such as active component, wetting agent, dispersant, interleaving agent, disintegrant, stabilizer, adhesive key element are constituted.It is helped Agent and carrier, and the compound method of precursor (preformed objects before granulation) are analogous to wettable powder and suspending agent substantially.
C suspending agents (SC), are the not dispersion of water soluble solid agricultural chemicals or immiscible liquids agricultural chemicals in water or oil.By activity Composition, auxiliary agent (wetting dispersing agent, thickener, stabilizer, pH adjusting agent, defoamer, antifreezing agent) are obtained through soybean dietary fiber. Wetting dispersing agent can be naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), lignin and its derivative sulfonate (M-9, POLYFON etc.), alkylphenol-polyethenoxy base ether sulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, alkyl Phenol polyethenoxy base phosphate, carboxymethylcellulose calcium (CMC), polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), condensation phosphorus One or more in hydrochlorate etc., content 0.1~12%；Thickener and stabilizer can be xanthan gum (XG), polyvinyl alcohol (PVA), one or more in Magnesiumaluminumsilicate etc., content 0.05~0.5%；PH adjusting agent and defoamer optionally can add in right amount Plus.
D dry suspending agents (Dry Flowable, abbreviation DF), are that can be comparatively fast disintegrated in water, disperse to form higher suspension dispersion The granule of system.Preparation method：The preparation obtained by the dehydration of suspending agent.Its auxiliary agent and carrier, and precursor (are dehydrated Preceding preformed objects) compound method it is consistent with suspending agent.
Bactericidal composition of the present invention is applied to the pesticidal preparations for preparing controlling plant diseases.It is particularly useful for making Prevent and treat rice sheath blight disease, rice blast, false smut, wheat rust, powdery mildew, root rotof flax, wheat sharp eyespot, rice banded sclerotial blight The pesticidal preparations of disease, root rot, sclerotinia rot of colza, maize head smut, capsicum wilt, cucurbits fusarium wilt etc..
Bactericidal composition of the present invention can be provided in finished product preparation form, i.e., each material has been mixed in bactericidal composition Close, or the composition of bactericidal composition can also be provided with unit dose form, directly directly be mixed in bucket or tank using preceding, so Required concentration is diluted to afterwards.
The method that bactericidal composition of the present invention is applied to controlling disease, active principle A and component B are with 1:100- 100:1 weight ratio is applied, and further dispenser ratio is 1:50-50:1, further dispenser ratio is 1:50-50:1, it can be pressed Apply, be administered simultaneously in proportion or apply successively in proportion after aforementioned proportion mixing.Specific method of application can be powder injection process, spray Mist method, smog method, fumigating system and Seed Treatment and soil treatment, ultra-low volume spray, mix fertilizer, spread fertilizer over the fields, pouring root.
Compared with prior art, the beneficial effects of the present invention are：
1st, compared with single dose, compounding has obvious synergistic function, improves prevention effect；
2nd, composition is made up of two kinds of different active ingredients of mechanism of action, and bactericidal effect is good, is conducive to overcoming and delays The drug-fast generation of germ；
3rd, medicament mixture reduces dosage, so as to reduce cost and alleviate the pollution to environment.
Rationally, treatment adds protective effect to instant component, and drug cost is low, and its activity and bactericidal effect are not each components The simple superposition of activity, but have significant synergistic effect, it is good to crop safety, meet the security requirement of pesticidal preparations.
Specific embodiment
To make the technical problem to be solved in the present invention, technical scheme and advantage clearer, specific embodiment will be used below It is described in detail, but the present invention is limited to absolutely not these examples.As described below is only the present invention preferably embodiment, is only used to The present invention is explained, therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that all of the invention Any modification, equivalent and improvement made within spirit and principle etc., should be included within the scope of the present invention. Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
The combination of active principles of different agricultural chemicals is made agricultural chemicals, is to develop and develop novel pesticide and preventing and treating at present agriculturally A kind of effective and quick way of resistance germ.After the pestsides synthesis of different cultivars, a large amount of three kinds of effects classes are typically exhibited Type：Summation action, synergistic effect and antagonism.Compound synergic is formulated well, can significantly improve actual prevention effect, drop The usage amount of low agricultural chemicals, is the important means of integrated control disease so as to help to delay the drug-fast generation speed of germ.
With shenqinmycin (A) for a kind of active ingredient, active bactericide (B) is another active ingredient to the present composition, Being combined between them has obvious synergistic function to plant disease, and not exclusively two kinds the simple of pharmacy effect are folded Plus, this can be clearly understood that from indoor virulence test result.
In order to prevent and treat the tomato late blight in agricultural production, inventor is with shenqinmycin and the cyanogen of sulphaguanidine azoles fungicide The synergy that white azoles is compounded is studied, and specific method is：
It is test object that experiment uses tomato late blight.
The test medicine that active compound is configured to need, test method (blade method) reference《People's Republic of China's agricultural row Industry standard》.Experiment is cultivated with pathogen on suitable culture medium, after sporangium to be generated, with spore under aseptic washing, is used Double gauze is filtered, and is made sporangia suspension, is placed under 4 DEG C of low temperature, dark processing 0.5h-3h, is tried it and is discharged travelling spore Son, and adjust spore concentration to 1*105Individual spore/L, it is standby；Or directly prepare 1105Individual sporangium/ml, suspension is used as inoculation Body.The test medicine that active compound is configured to need, medicament is uniformly sprayed in vacuum side of blade, after after liquid natural air drying, by respectively Treatment vacuum side of blade upward, is emitted in moisture preservation box after being marked by treatment.Often treatment is no less than 10 leaves, 4 repetitions, and sets only Blank is done in the treatment that effect composition is not contained containing solvent and surfactant.Sprayed with sporangium or zoospore suspension Inoculation.24h or so is inoculated with after protectiveness experiment generally chemicals treatment；The general 24h before chemicals treatment of curative tests Inoculation.Replace in each 12H of daily continuous illumination/dark after inoculation, temperature is 18 DEG C.And after inoculation there be holding blade face in 24H Moisture film, later relative humidity be more than 90% under conditions of cultivate 7 days.When blank disease leaf rate reaches more than 50%, point Incidence is managed in level investigation everywhere.Stage division is：0 grade：Do not fall ill；1 grade：Only a small amount of small scab on blade, scab accounts for leaf Below area 10%；3 grades：Scab accounts for leaf area 10%-25% on blade；5 grades：Scab accounts for leaf area 25%-50% on blade； 7 grades：Scab accounts for leaf area more than 50% on blade；9 grades：Full leaf morbidity is withered.
According to blank incidence, each treatment disease index is investigated, calculate preventive effect.By the probit value of inhibiting rate and Linear regression analysis between the logarithm value of series concentration, obtains the EC50 values of each medicament, and mixture is calculated with co-toxicity coefficient meter Co-toxicity coefficient (CTC), activity of the reagent agent to germ is evaluated with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect；CTC≤80 show as antagonism；80 ﹤ CTC ﹤ 120 show as summation action.
The shenqinmycin of table 1 and cyazofamid compound the Toxicity Determination to tomato late blight
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shenqinmycin (A) —— Y=1.6974X+3.8406 4.8198 ——
Cyazofamid (B1) —— Y=1.1207X+3.0833 51.3205 ——
A:B1 1:100 Y=0.8133X+3.7145 38.0600 123.08
A:B1 1:50 Y=0.8392X+3.7158 33.9062 127.29
A:B1 1:25 Y=0.8094X+3.8296 27.9272 134.08
A:B1 1:1 Y=0.7258X+4.4771 5.2537 167.72
A:B1 25:1 Y=0.6666X+4.6275 3.6275 137.67
A:B1 50:1 Y=0.7039X+4.6006 3.6931 132.88
A:B1 100:1 Y=0.7284X+4.5749 3.83361 126.87
Toxicity Determination result table 1 shown, 1 is compared in weight:100-100:1, particularly 1:50-50:In the range of 1, Especially compare 1 in weight：25-25：Shenqinmycin with cyazofamid compounding there is obvious synergy to make to plant disease in the range of 1 With the synergistic effect of compounding becomes apparent, and prevention effect is excellent and is better than single dose kind.
In order to prevent and treat the sclerotinia rot of colza in agricultural production, inventor is with shenqinmycin He oxazole series bactericidal agent hymexazos The synergy for being compounded is studied, and specific method is：
It is test object that experiment uses sclerotinia rot of colza.
The test medicine that active compound is configured to need, with reference to mycelial growth rate method, the preparation of strain：It is sterilized About 10mLPDA culture mediums are poured into culture medium.Aseptically, a mycelia is taken from the strain inclined plane of Sclerotinia sclerotiorum Block, is placed in the middle of culture dish.Continuous culture several generations, then before the test 3d, in inoculation once, trained under the conditions of 24~26 DEG C Support, it is standby.The making of toxic flat board, after the PDA culture medium that will first prepare is dissolved, places cooling.Aseptically, will Liquid 1mL is added in sterilized culture dish, then in the culture dish of the existing liquid of use pipette, extract 9mL culture mediums addition, Rocking gently shakes up (left 3 enclose, and right 3 enclose), is made the uniform flat board of thin and thick.Connect bacterium：The pathogen punching that will be ready for Device makes bacteria cake, is then moved on the pastille flat board for having solidified with transfer needle picking bacteria cake, and mycelia one faces down.Mark, puts 24 Cultivated in~26 DEG C of incubators.Treat that control group colony diameter is long to 6cm or so, crossing method investigates the data of other treatment simultaneously Record.
According to blank incidence, lesion diameter is recorded, calculate the percentage that each treatment suppresses scab growth, passed through Linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtains the EC50 values of each medicament, uses common poison Coefficient table calculates the co-toxicity coefficient (CTC) of mixture, and activity of the reagent agent to germ is evaluated with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect；CTC≤80 show as antagonism；80 ﹤ CTC ﹤ 120 show as summation action.
The shenqinmycin of table 2 is He hymexazo compounds the Toxicity Determination to sclerotinia rot of colza
Toxicity Determination result table 2 shown, 1 is compared in weight:100-100:1, particularly 1:50-50:In the range of 1, Especially compare 1 in weight：25-25：There is shenqinmycin Yu hymexazos compounding obvious synergy to make to plant disease in the range of 1 With prevention effect is excellent and is better than single dose kind.
In order to prevent and treat the wheat sharp eyespot in agricultural production, inventor is with shenqinmycin and the fludioxonil of pyrroles's series bactericidal agent The synergy for being compounded is studied, and specific method is：
It is test object that experiment uses wheat sharp eyespot.
The test medicine that active compound is configured to need, test method：Fungicide bioassay pattern -- Broad Bean Leaves method, With reference to《People's Republic of China's agricultural industry criteria》.Choose broad bean susceptible variety potted plant, clip same section, growing way is consistent, With petiolate blade, put in culture dish, moisturizing is standby, experimental pharmacy is diluted using active compound with water dissolves, 5 quality are set Concentration, 5S is fully infiltrated by blade in pre-configured good liquid, drains unnecessary liquid, after natural air drying, after treatment mark Moisturizing culture, it is blank that experiment sets the treatment without medicament.The one side that a diameter of 5mm bacteria cakes have mycelia is connect with inoculator Plant in treatment central vane, each treatment 30 leaf of inoculation.Protectiveness tests 24h inoculations, curative tests after chemicals treatment The 24h inoculations before chemicals treatment.Growth cabinet or constant temperature are placed in after inoculation to be had in illumination humidistat in fact, Celsius in temperature 26 Cultivated under conditions of -28 degrees Celsius of degree, relative humidity 80%-90%.According to blank incidence, recorded with kind of calliper Each vaccination scab length and width, represents that lesion diameter records lesion diameter (unit mm) according to tune with length and width average value Data are looked into, by preventing and treating result publicity：Preventive effect=(blank diameter-treatment diameter)/blank diameter * 100, calculates each treatment and suppresses disease The percentage of spot growth, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtains each The EC50 values of medicament, the co-toxicity coefficient (CTC) of mixture is calculated with co-toxicity coefficient meter, and reagent agent is evaluated to germ with this Activity.
According to blank incidence, lesion diameter is recorded, calculate the percentage that each treatment suppresses scab growth, passed through Linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtains the EC50 values of each medicament, uses common poison Coefficient table calculates the co-toxicity coefficient (CTC) of mixture, and activity of the reagent agent to germ is evaluated with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect；CTC≤80 show as antagonism；80 ﹤ CTC ﹤ 120 show as summation action.
The shenqinmycin of table 3 and fludioxonil compound the Toxicity Determination to wheat sharp eyespot
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shenqinmycin (A) —— Y=1.9338X+2.0570 33.2586 ——
Fludioxonil (B3) —— Y=1.9057X+2.2920 26.3637 ——
A：B3 1:100 Y=1.2830X+3.2849 21.5031 121.62
A：B3 1:50 Y=1.0906X+3.6234 18.2905 144.72
A：B3 1:25 Y=0.9393X+3.8792 15.6047 170.30
A：B3 1:1 Y=0.9717X+3.8323 15.6883 187.48
A：B3 25:1 Y=1.4046X+3.2208 18.4823 178.15
A：B3 50:1 Y=15701X+2.8471 23.5048 140.77
A：B3 100:1 Y=17365X+2.5490 25.7910 128.61
Toxicity Determination result table 3 shown, 1 is compared in weight:100-100:1, particularly 1:50-50:In the range of 1, Especially compare 1 in weight：25-25：Shenqinmycin with fludioxonil compounding there is obvious synergy to make to plant disease in the range of 1 With prevention effect is excellent and is better than single dose kind.
In order to prevent and treat the downy mildew of lettuce in agricultural production, inventor is with shenqinmycin and the triethylphosphine of organophosphorus fungicide The synergy that sour aluminium is compounded is studied, and specific method is：
It is test object that experiment uses downy mildew of lettuce.
The test medicine that active compound is configured to need, test method (plate blade method) reference《People's Republic of China's agriculture Industry professional standard》.Choose lettuce susceptible variety potted plant, clip same section, growing way is consistent, with petiolate blade, puts culture In ware, moisturizing is standby.By single dose and each Mixed Pharmacy set 5 various concentrations gradients (on the basis of pilot study result, root Combine different with proportioning according to different agents, concentration sets also different, and bacteriostasis rate sets in the range of 5%~95%).Will Liquid is uniformly sprayed in vacuum side of blade, after after liquid natural air drying, each treatment blade and blade is dorsad gone up, by discharge after treatment mark Standby in moisture preservation box, it is blank that experiment sets the treatment without medicament.It is inoculated with ready Fresh spores drop 10uL In vacuum side of blade, dripped per blade inoculation 4, often treatment is no less than 5 leaves, protectiveness is tested 24 hours after chemicals treatment and is inoculated with, Curative tests are inoculated with for 24 hours before chemicals treatment, then cover ware lid, are placed in temperature for 17~22 DEG C of relative humidity 90% 5-7d is cultivated in constant temperature and humidity incubator above, daily continuous illumination/night 12h replaces；According to blank morbidity feelings Condition, records lesion diameter, calculates the percentage that each treatment suppresses scab growth, by the probit value of inhibiting rate and series concentration Linear regression analysis between logarithm value, obtains the EC50 values of each medicament, and the co-toxicity coefficient of mixture is calculated with co-toxicity coefficient meter (CTC) activity of the reagent agent to germ, is evaluated with this.
According to blank incidence, lesion diameter is recorded, calculate the percentage that each treatment suppresses scab growth, passed through Linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtains the EC50 values of each medicament, uses common poison Coefficient table calculates the co-toxicity coefficient (CTC) of mixture, and activity of the reagent agent to germ is evaluated with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect；CTC≤80 show as antagonism；80 ﹤ CTC ﹤ 120 show as summation action.
The shenqinmycin of table 4 and phosethyl-Al compound the Toxicity Determination to downy mildew of lettuce
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shenqinmycin (A) —— Y=1.8664X+1.9415 43.5238 ——
Phosethyl-Al (B4) —— Y=1.8435X+1.2674 105.8708 ——
A：B4 1:100 Y=1.7797X+1.7255 69.1631 150.93
A：B4 1:50 Y=1.5902X+2.2177 56.1834 183.29
A：B4 1:25 Y=1.2482X+2.8734 50.5453 198.54
A：B4 1:1 Y=1.3733X+3.1105 23.7598 259.62
A：B4 25:1 Y=1.3500X+3.0151 29.5324 150.79
A：B4 50:1 Y=1.0815X+3.3718 32.0268 137.49
A：B4 100:1 Y=0.8909X+3.6372 33.8661 129.27
Toxicity Determination result table 4 shown, 1 is compared in weight:100-100:1, particularly 1:50-50:In the range of 1, Especially compare 1 in weight：25-25：Shenqinmycin has obvious synergy to plant disease with phosethyl-Al compounding in the range of 1 Effect, prevention effect is excellent and is better than single dose kind.
The result of Toxicity Determination table 1- tables 4 shown, 1 is compared in weight:100-100:1, particularly 1:50-50:1 scope Interior, shenqinmycin is with cyazofamid, shenqinmycin Yu hymexazo, shenqinmycin and fludioxonil, shenqinmycin and phosethyl-Al are multiple Pairing plant disease has obvious synergistic effect, and prevention effect is excellent and is better than single dose kind.Especially compare 1 in weight：25- 25：In the range of 1, shenqinmycin is with cyazofamid, shenqinmycin Yu hymexazo shenqinmycin and fludioxonil, shenqinmycin and three second The synergistic effect of aluminum phosphate compounding becomes apparent.
To make the technical problem to be solved in the present invention, technical scheme and advantage clearer, 1 is compared in weight:100~100: In the range of 1, the embodiment that shenqinmycin is compounded with cyazofamid, hymexazos, fludioxonil or phosethyl-Al respectively is built, carried out in detail Thin description, but the present invention is limited to absolutely not these examples.
In addition, inventor is also found through experiments that, shenqinmycin respectively with cyazofamid, hymexazos, fludioxonil or triethylphosphine The compounding effect of sour aluminium, better than other antibioticses bactericide and the compounding of cyazofamid, hymexazos, fludioxonil or phosethyl-Al Effect.In order to clearly demonstrate this problem, 1 is compared in weight:100~100:In the range of 1, other antibioticses bactericide are built The comparative example compounded with cyazofamid, hymexazos, fludioxonil or phosethyl-Al, is described in detail.But because length is limited, only Representative several comparative examples can be enumerated.It should be pointed out that not being limited in limited comparative example.
Bactericidal composition of the invention can be prepared into any one formulation of suitable agricultural use with known method, than Preferable formulation is wettable powder, aqueous suspension agent, oil-suspending agent, suspended emulsion, water dispersible granules.Percentage in all formulas It is weight percentage.The processing technology of the various formulations of the present composition is prior art, can be had according to different situations Changed.
Present invention is described further with reference to embodiment.
First, shenqinmycin and cyazofamid are compounded
Embodiment 1：20.2% shenqinmycin cyazofamid suspending agent (1:100)
Component:Shenqinmycin 0.2%, cyazofamid 20%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinethene 4%, 1,2- the third two Alcohol 6%, XG0.32%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, it is well mixed through high speed shear, then be sanded 2-3 hours by sand mill, make to put down Equal particle diameter reaches 1-5 microns, you can 20.2% shenqinmycin cyazofamid suspending agent is obtained.
Comparative example 1:20.2% polyoxin cyazofamid suspending agent (1:100)
Component:Polyoxin 0.2%, cyazofamid 20%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinethene 4%, 1,2- the third two Alcohol 6%, XG0.32%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.Preparation method：Ibid.
Embodiment 2:16.8% shenqinmycin cyazofamid water dispersible granules (1:55)
Component:Shenqinmycin 0.3%, cyazofamid 16.5%, lignin sodium naphthalene sulfonate 6%, polycarboxylic acids sodium 3%, anhydrous sulphur Sour sodium 10%, kaolin complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 16.8% shenqinmycin cyazofamid water dispersible granules are obtained.
Embodiment 3:25.5% shenqinmycin cyazofamid wettable powder (1:50)
Component:Shenqinmycin 0.5%, cyazofamid 25%, lauryl sodium sulfate 4%, sodium lignin sulfonate 6%, hard charcoal Black 6%, precipitated calcium carbonate complements to 100%.
Preparation method：Each component is well mixed, mechanical crushing is first passed through, then by after air-flow crushing, being well mixed, i.e., 25.5% shenqinmycin cyazofamid wettable powder can be obtained.
Comparative example 2：25.5% kasugarnycin cyazofamid wettable powder (1:50)
Component:Kasugarnycin 0.5%, cyazofamid 25%, lauryl sodium sulfate 4%, sodium lignin sulfonate 6%, hard charcoal Black 6%, precipitated calcium carbonate complements to 100%.Preparation method：Ibid.
Embodiment 4:35% shenqinmycin cyazofamid dry suspending agent (1:34)
Component:Shenqinmycin 1%, cyazofamid 34%, lignin sodium naphthalene sulfonate 10%, sodium lignin sulfonate 8%, white carbon 10%, organic silicon defoamer 0.4%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method：1.5-2.0 times of water of preparation amount of preparation are added, each component is mixed, it is equal through high speed shear mixing It is even, then be sanded 2-3 hours by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 35% Shen piperazine mould Plain cyazofamid dry suspending agent.
Embodiment 5:26% shenqinmycin cyazofamid water dispersible granules (1:25)
Component:Shenqinmycin 1%, cyazofamid 25%, sodium naphthalene sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8% is sliding Stone flour complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 26% shenqinmycin cyazofamid water dispersible granules are obtained.
Comparative example 3:26% Ningnanmycin cyazofamid water dispersible granules (1:25)
Component:Ningnanmycin 1%, cyazofamid 25%, sodium naphthalene sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8% is sliding Stone flour complements to 100%.Preparation method：Ibid.
Embodiment 6:32% shenqinmycin cyazofamid suspending agent (1:15)
Component:Shenqinmycin 2%, cyazofamid 30%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 4%, fatty alcohol polyoxy second Alkene ether 3%, ethylene glycol 6%, XG0.25%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, it is well mixed through high speed shear, then be sanded 2-3 hours by sand mill, make to put down Equal particle diameter reaches 1-5 microns, you can 16% shenqinmycin cyazofamid suspending agent is obtained.
Embodiment 7：20% shenqinmycin cyazofamid wettable powder (1:1)
Component:Shenqinmycin 10%, cyazofamid 10%, neopelex 4%, dodecyl naphthalene sulphonate formaldehyde Condensation product 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.
Preparation method：Each component is well mixed, mechanical crushing is first passed through, then by after air-flow crushing, being well mixed, i.e., 20% shenqinmycin cyazofamid wettable powder can be obtained.
Comparative example 4：20% polyoxin cyazofamid wettable powder (1:1)
Component:Polyoxin 10%, cyazofamid 10%, neopelex 4%, dodecyl naphthalene sulphonate formaldehyde Condensation product 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.Preparation method：Ibid.
Comparative example 5：20% kasugarnycin cyazofamid wettable powder (1:1)
Component:Kasugarnycin 10%, cyazofamid 10%, neopelex 4%, dodecyl naphthalene sulphonate formaldehyde Condensation product 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.Preparation method：Ibid.
Embodiment 8:21% shenqinmycin cyazofamid water dispersible granules (19:2)
Component:Shenqinmycin 19%, cyazofamid 2%, lauryl sodium sulfate 3%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 6%, talcum powder complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 21% shenqinmycin cyazofamid water dispersible granules are obtained.
Embodiment 9：16% shenqinmycin cyazofamid dry suspending agent (15:1)
Component:Shenqinmycin 15%, cyazofamid 1%, lignin sodium naphthalene sulfonate 8%, sodium lignin sulfonate 8%, white carbon 6%, organic silicon defoamer 0.4%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method：1.5-2.0 times of water of preparation amount of preparation are added, each component is mixed, it is equal through high speed shear mixing It is even, then be sanded 2-3 hours by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 16% Shen piperazine mould Plain cyazofamid dry suspending agent.
Embodiment 10:26% shenqinmycin cyazofamid water dispersible granules (25:1)
Component:Shenqinmycin 25%, cyazofamid 1%, lauryl sodium sulfate 3%, lignin sodium naphthalene sulfonate 6% is anhydrous Sodium sulphate 8%, kaolin complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 26% shenqinmycin cyazofamid water dispersible granules are obtained.
Comparative example 6:26% Ningnanmycin cyazofamid water dispersible granules (25:1)
Component:Ningnanmycin 25%, cyazofamid 1%, lauryl sodium sulfate 3%, lignin sodium naphthalene sulfonate 6% is anhydrous Sodium sulphate 8%, kaolin complements to 100%.Preparation method：Ibid.
Embodiment 11:23% shenqinmycin cyazofamid suspending agent (45:1)
Component:Shenqinmycin 22.5%, cyazofamid 0.5%, polycarboxylic acids sodium 4%, fatty alcohol oxygen vinethene 3%, ethylene glycol 5%, XG0.3%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, it is well mixed through high speed shear, then be sanded 2-3 hours by sand mill, make to put down Equal particle diameter reaches 1-5 microns, you can 23% shenqinmycin cyazofamid suspending agent is obtained.
Embodiment 12:20.4% shenqinmycin cyazofamid suspending agent (50:1)
Component:Shenqinmycin 20%, cyazofamid 0.4%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinethene 3%, 1,2- the third two Alcohol 5%, XG0.3%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, it is well mixed through high speed shear, then be sanded 2-3 hours by sand mill, make to put down Equal particle diameter reaches 1-5 microns, you can 20.4% shenqinmycin cyazofamid suspending agent is obtained.
Comparative example 7：20.4% kasugarnycin cyazofamid suspending agent (50:1)
Component:Kasugarnycin 20%, cyazofamid 0.4%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinethene 3%, 1,2- the third two Alcohol 5%, XG0.3%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.Preparation method：Ibid.
Embodiment 13:20.2% shenqinmycin cyazofamid wettable powder (100:1)
Component：Shenqinmycin 20%, cyazofamid 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, White carbon 6%, talcum powder complements to 100%.
Preparation method：Each component is well mixed, mechanical crushing is first passed through, then by after air-flow crushing, being well mixed, i.e., 20.2% shenqinmycin cyazofamid wettable powder can be obtained.
Comparative example 8：20.2% polyoxin cyazofamid wettable powder (100:1)
Component：Polyoxin 20%, cyazofamid 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, White carbon 6%, talcum powder complements to 100%.Preparation method：Ibid.
2nd, shenqinmycin is Yu hymexazo is compounded
Embodiment 14：20.2% Shen piperazine Mei Su hymexazos water dispersible granules (1:100)
Component:The , hymexazos 20% of shenqinmycin 0.2%, lauryl sodium sulfate 3%, polycarboxylic acids sodium 4%, anhydrous slufuric acid Sodium 8%, kaolin complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 20.2% Shen piperazine Mei Su hymexazo water dispersible granules are obtained.
Comparative example 9:The Mei Su hymexazos of Kang Duo in the of 20.2% water dispersible granules (1:100)
Component:The , hymexazos 20% of polyoxin 0.2%, lauryl sodium sulfate 3%, polycarboxylic acids sodium 4%, anhydrous slufuric acid Sodium 8%, kaolin complements to 100%.Preparation method：Ibid.
Embodiment 15:16.8% Shen piperazine Mei Su hymexazos suspending agent (1:55)
Component:Shenqinmycin 0.3%, hymexazo 16.5%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 3%, poly alkyl alcohol Oxygen vinethene 3%, ethylene glycol 6%, XG 0.32%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water is complemented to 100%.
Preparation method：Each component is mixed, it is well mixed through high speed shear, then be sanded 2-3 hours by sand mill, make to put down Equal particle diameter reaches 1-5 microns, you can 16.8% Shen piperazine Mei Su hymexazo suspending agents are obtained.
Embodiment 16:25.5% Shen piperazine Mei Su hymexazos wettable powder (1:50)
Component:The , hymexazos 25% of shenqinmycin 0.5%, sodium naphthalene sulfonate 5%, lauryl sodium sulfate 3%, white carbon 10%, talcum powder complements to 100%.
Preparation method：Each component is well mixed, mechanical crushing is first passed through, then by after air-flow crushing, being well mixed, i.e., 25.5% Shen piperazine Mei Su hymexazo wettable powders can be obtained.
Comparative example 10：The peaceful Mei Su hymexazos wettable powder (1 of 25.5% Chinese parasol tree:50)
Component:The , hymexazos 25% of tetramycin 0.5%, sodium naphthalene sulfonate 5%, lauryl sodium sulfate 3%, white carbon 10%, talcum powder complements to 100%.Preparation method：Ibid.
Embodiment 17:35% Shen piperazine Mei Su hymexazos dry suspending agent (1:34)
Component:The , hymexazos 34% of shenqinmycin 1%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 8%, organosilicon Defoamer 0.4%, Sodium Benzoate 0.1%, white carbon 10%, kaolin complements to 100%.Soft water 150-200%.
Preparation method：1.5-2.0 times of water of preparation amount of preparation are added, each component is mixed, it is equal through high speed shear mixing It is even, then be sanded 2-3 hours by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 35% Shen piperazine mould Su hymexazo dry suspending agents.
Embodiment 18:26% Shen piperazine Mei Su hymexazos water dispersible granules (1:25)
Component:The , hymexazos 25% of shenqinmycin 1%, neopelex 4%, polycarboxylic acids sodium 4%, anhydrous slufuric acid Sodium 8%, talcum powder complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 26% Shen piperazine Mei Su hymexazo water dispersible granules are obtained.
Comparative example 11:The Mei Su hymexazos of Kang Duo in the of 26% water dispersible granules (1:25)
Component:The , hymexazos 25% of polyoxin 1%, neopelex 4%, polycarboxylic acids sodium 4%, anhydrous slufuric acid Sodium 8%, talcum powder complements to 100%.Preparation method：Ibid.
Embodiment 19:32% Shen piperazine Mei Su hymexazos suspending agent (1:15)
Component:The , hymexazos 30% of shenqinmycin 2%, polycarboxylic acids sodium 4%, AEO 3%, ethylene glycol 6%, XG 0.26%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, it is well mixed through high speed shear, then be sanded 2-3 hours by sand mill, make to put down Equal particle diameter reaches 1-5 microns, you can 32% Shen piperazine Mei Su hymexazo suspending agents are obtained.
Embodiment 20：20% Shen piperazine Mei Su hymexazos wettable powder (1:1)
Component:The , hymexazos 10% of shenqinmycin 10%, lignin sodium naphthalene sulfonate 6%, neopelex 3%, in vain Carbon black 10%, kaolin complements to 100%.
Preparation method：Each component is well mixed, mechanical crushing is first passed through, then by after air-flow crushing, being well mixed, i.e., 20% Shen piperazine Mei Su hymexazo wettable powders can be obtained.
Comparative example 12：The Mei Su hymexazos of Kang Duo in the of 20% wettable powder (1:1)
Component:The , hymexazos 10% of polyoxin 10%, lignin sodium naphthalene sulfonate 6%, neopelex 3%, in vain Carbon black 10%, kaolin complements to 100%.Preparation method：Ibid.
Embodiment 21:21% Shen piperazine Mei Su hymexazos water dispersible granules (19:2)
Component:The , hymexazos 2% of shenqinmycin 19%, dodecyl sodium sulfate 4%, lauryl sodium sulfate 4% is anhydrous Sodium sulphate 10%, talcum powder complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 21% Shen piperazine Mei Su hymexazo water dispersible granules are obtained.
Embodiment 22：16% Shen piperazine Mei Su hymexazos dry suspending agent (15:1)
Component:The , hymexazos 1% of shenqinmycin 15%, polycarboxylic acids sodium 4%, lignin sodium naphthalene sulfonate 10%, organosilicon froth breaking Agent 0.4%, Sodium Benzoate 0.1%, white carbon 10%, kaolin complements to 100%.Soft water 150-200%.
Preparation method：1.5-2.0 times of water of preparation amount of preparation are added, each component is mixed, it is equal through high speed shear mixing It is even, then be sanded 2-3 hours by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 16% Shen piperazine mould Su hymexazo dry suspending agents.
Embodiment 23:26% Shen piperazine Mei Su hymexazos water dispersible granules (25:1)
Component:The , hymexazos 1% of shenqinmycin 25%, polycarboxylic acids sodium 4%, lauryl sodium sulfate 4%, anhydrous sodium sulfate 10%, kaolin complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 26% Shen piperazine Mei Su hymexazo water dispersible granules are obtained.
Comparative example 13:The peaceful Mei Su hymexazos water dispersible granules (25 of 26% Chinese parasol tree:1)
Component:The , hymexazos 1% of tetramycin 25%, polycarboxylic acids sodium 4%, lauryl sodium sulfate 4%, anhydrous sodium sulfate 10%, kaolin complements to 100%.Preparation method：Ibid.
Embodiment 24:23% Shen piperazine Mei Su hymexazos suspending agent (45:1)
Component:The , hymexazos 0.5% of shenqinmycin 22.5%, polycarboxylic acids sodium 3%, OPEO sodium sulfonate 3%, ethylene glycol 6%, XG 0.3%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, it is well mixed through high speed shear, then be sanded 2-3 hours by sand mill, make to put down Equal particle diameter reaches 1-5 microns, you can 23% Shen piperazine Mei Su hymexazo suspending agents are obtained.
Embodiment 25:20.4% Shen piperazine Mei Su hymexazos dry suspending agent (50:1)
Component:The , hymexazos 0.4% of shenqinmycin 20%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 10% is organic Silicon defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 6%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method：1.5-2.0 times of water of preparation amount of preparation are added, each component is mixed, it is equal through high speed shear mixing It is even, then be sanded 2-3 hours by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 20.4% Shen piperazine mould Su hymexazo dry suspending agents.
Comparative example 14：The Mei Su hymexazos of Kang Duo in the of 20.4% dry suspending agent (50:1)
Component:The , hymexazos 0.4% of polyoxin 20%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 10% is organic Silicon defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 6%, talcum powder complements to 100%.Soft water 150-200%.Preparation side Method：Ibid.
Embodiment 26:20.2% Shen piperazine Mei Su hymexazos wettable powder (100:1)
Component：The , hymexazos 0.2% of shenqinmycin 20%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, White carbon 8%, kaolin complements to 100%.
Preparation method：Each component is well mixed, mechanical crushing is first passed through, then by after air-flow crushing, being well mixed, i.e., 20.2% Shen piperazine Mei Su hymexazo wettable powders can be obtained.
Comparative example 15：The peaceful Mei Su hymexazos wettable powder (100 of 20.2% Chinese parasol tree:1)
Component：The , hymexazos 0.2% of tetramycin 20%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, White carbon 8%, kaolin complements to 100%.Preparation method：Ibid.
3rd, shenqinmycin and fludioxonil are compounded
Embodiment 27：20.2% shenqinmycin fludioxonil suspending agent (1:100)
Component:Shenqinmycin 0.2%, fludioxonil 20%, polycarboxylic acids sodium 3%, AEO 3%, ethylene glycol 6%, XG 0.32%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, it is well mixed through high speed shear, then be sanded 2-3 hours by sand mill, make to put down Equal particle diameter reaches 1-5 microns, you can 20.2% shenqinmycin fludioxonil suspending agent is obtained.
Comparative example 16:20.2% polyoxin fludioxonil suspending agent (1:100)
Component:Polyoxin 0.2%, fludioxonil 20%, polycarboxylic acids sodium 3%, AEO 3%, ethylene glycol 6%, XG 0.32%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.Preparation method：Ibid.
Embodiment 28:16.8% shenqinmycin fludioxonil water dispersible granules (1:55)
Component:Shenqinmycin 0.3%, fludioxonil 16.5%, neopelex 4%, polycarboxylic acids sodium 4% is anhydrous Sodium sulphate 8%, talcum powder complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 16.8% shenqinmycin fludioxonil water dispersible granules are obtained.
Comparative example 17:16.8% jinggangmeisu fludioxonil water dispersible granules (1:55)
Component:Jinggangmeisu 0.3%, fludioxonil 16.5%, neopelex 4%, polycarboxylic acids sodium 4% is anhydrous Sodium sulphate 8%, talcum powder complements to 100%.Preparation method：Ibid.
Embodiment 29:25.5% shenqinmycin fludioxonil wettable powder (1:50)
Component:Shenqinmycin 0.5%, fludioxonil 25%, neopelex 4%, naphthalenesulfonic acid-formaldehyde condensate 8%, white carbon 6%, precipitated calcium carbonate complements to 100%.
Preparation method：Each component is well mixed, mechanical crushing is first passed through, then by after air-flow crushing, being well mixed, i.e., 25.5% shenqinmycin fludioxonil wettable powder can be obtained.
Comparative example 18：25.5% kasugarnycin fludioxonil wettable powder (1:50)
Component:Kasugarnycin 0.5%, fludioxonil 25%, neopelex 4%, naphthalenesulfonic acid-formaldehyde condensate 8%, white carbon 6%, precipitated calcium carbonate complements to 100%.Preparation method：Ibid.
Embodiment 30:35% shenqinmycin fludioxonil dry suspending agent (1:34)
Component:Shenqinmycin 1%, fludioxonil 34%, lignin sodium naphthalene sulfonate 10%, polycarboxylic acids sodium 4%, white carbon 5%, Organic silicon defoamer 0.4%, Sodium Benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method：1.5-2.0 times of water of preparation amount of preparation are added, each component is mixed, it is equal through high speed shear mixing It is even, then be sanded 2-3 hours by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 35% Shen piperazine mould Plain fludioxonil dry suspending agent.
Embodiment 31:26% shenqinmycin fludioxonil water dispersible granules (1:25)
Component:Shenqinmycin 1%, fludioxonil 25%, sodium naphthalene sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 10% is sliding Stone flour complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 26% shenqinmycin fludioxonil water dispersible granules are obtained.
Comparative example 19:26% Ningnanmycin fludioxonil water dispersible granules (1:25)
Component:Ningnanmycin 1%, fludioxonil 25%, sodium naphthalene sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 10% is sliding Stone flour complements to 100%.Preparation method：Ibid.
Embodiment 32:32% shenqinmycin fludioxonil suspending agent (1:15)
Component:Shenqinmycin 2%, fludioxonil 30%, polycarboxylic acids sodium 3%, OPEO sodium sulfonate 3%, second Glycol 6%, XG 0.25%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, it is well mixed through high speed shear, then be sanded 2-3 hours by sand mill, make to put down Equal particle diameter reaches 1-5 microns, you can 32% shenqinmycin fludioxonil suspending agent is obtained.
Comparative example 20:32% tetramycin fludioxonil suspending agent (1:15)
Component:Tetramycin 2%, fludioxonil 30%, polycarboxylic acids sodium 3%, OPEO sodium sulfonate 3%, second Glycol 6%, XG 0.25%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.Preparation method：Together On.
Embodiment 33：20% shenqinmycin fludioxonil wettable powder (1:1)
Component:Shenqinmycin 10%, fludioxonil 10%, polycarboxylic acids sodium 5%, lauryl sodium sulfate 3%, white carbon 8%, Precipitated calcium carbonate complements to 100%.
Preparation method：Each component is well mixed, mechanical crushing is first passed through, then by after air-flow crushing, being well mixed, i.e., 20% shenqinmycin fludioxonil wettable powder can be obtained.
Comparative example 21：20% polyoxin fludioxonil wettable powder (1:1)
Component:Polyoxin 10%, fludioxonil 10%, polycarboxylic acids sodium 5%, lauryl sodium sulfate 3%, white carbon 8%, Precipitated calcium carbonate complements to 100%.Preparation method：Ibid.
Comparative example 22：20% kasugarnycin fludioxonil wettable powder (1:1)
Component:Kasugarnycin 10%, fludioxonil 10%, polycarboxylic acids sodium 5%, lauryl sodium sulfate 3%, white carbon 8%, Precipitated calcium carbonate complements to 100%.Preparation method：Ibid.
Embodiment 34:21% shenqinmycin fludioxonil water dispersible granules (19:2)
Component:Shenqinmycin 19%, fludioxonil 2%, neopelex 4%, polycarboxylic acids sodium 4%, anhydrous slufuric acid Sodium 10%, talcum powder complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 21% shenqinmycin fludioxonil water dispersible granules are obtained.
Embodiment 35：16% shenqinmycin fludioxonil dry suspending agent (15:1)
Component:Shenqinmycin 15%, fludioxonil 1%, lignin sodium naphthalene sulfonate 8%, sodium lignin sulfonate 8%, white carbon 6%, organic silicon defoamer 0.4%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method：1.5-2.0 times of water of preparation amount of preparation are added, each component is mixed, it is equal through high speed shear mixing It is even, then be sanded 2-3 hours by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 16% Shen piperazine mould Plain fludioxonil dry suspending agent.
Comparative example 23：16% jinggangmeisu fludioxonil dry suspending agent (15:1)
Component:Jinggangmeisu 15%, fludioxonil 1%, lignin sodium naphthalene sulfonate 8%, sodium lignin sulfonate 8%, white carbon 6%, organic silicon defoamer 0.4%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.Preparation method： Ibid.
Embodiment 36:26% shenqinmycin fludioxonil water dispersible granules (25:1)
Component:Shenqinmycin 25%, fludioxonil 1%, lauryl sodium sulfate 3%, lignin sodium naphthalene sulfonate 6% is anhydrous Sodium sulphate 8%, kaolin complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 26% shenqinmycin fludioxonil water dispersible granules are obtained.
Comparative example 24:26% Ningnanmycin fludioxonil water dispersible granules (25:1)
Component:Ningnanmycin 25%, fludioxonil 1%, lauryl sodium sulfate 3%, lignin sodium naphthalene sulfonate 6% is anhydrous Sodium sulphate 8%, kaolin complements to 100%.Preparation method：Ibid.
Embodiment 37:23% shenqinmycin fludioxonil suspending agent (45:1)
Component:Shenqinmycin 22.5%, fludioxonil 0.5%, polycarboxylic acids sodium 4%, fatty alcohol oxygen vinethene 3%, ethylene glycol 5%, XG0.3%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, it is well mixed through high speed shear, then be sanded 2-3 hours by sand mill, make to put down Equal particle diameter reaches 1-5 microns, you can 23% shenqinmycin fludioxonil suspending agent is obtained.
Embodiment 38:20.4% shenqinmycin fludioxonil dry suspending agent (50:1)
Component:Shenqinmycin 20%, fludioxonil 0.4%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 10% is organic Silicon defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 6%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method：1.5-2.0 times of water of preparation amount of preparation are added, each component is mixed, it is equal through high speed shear mixing It is even, then be sanded 2-3 hours by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 20.4% Shen piperazine mould Plain fludioxonil dry suspending agent.
Comparative example 25：20.4% kasugarnycin fludioxonil dry suspending agent (50:1)
Component:Kasugarnycin 20%, fludioxonil 0.4%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 10% is organic Silicon defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 6%, talcum powder complements to 100%.Soft water 150-200%.Preparation side Method：Ibid.
Embodiment 39:20.2% shenqinmycin fludioxonil wettable powder (100:1)
Component：Shenqinmycin 20%, fludioxonil 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, White carbon 6%, talcum powder complements to 100%.
Preparation method：Each component is well mixed, mechanical crushing is first passed through, then by after air-flow crushing, being well mixed, i.e., 20.2% shenqinmycin fludioxonil wettable powder can be obtained.
Comparative example 26：20.2% polyoxin fludioxonil wettable powder (100:1)
Component：Polyoxin 20%, fludioxonil 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, White carbon 6%, talcum powder complements to 100%.
Preparation method：Ibid.
Comparative example 27：20.2% tetramycin fludioxonil wettable powder (100:1)
Component：Tetramycin 20%, fludioxonil 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, White carbon 6%, talcum powder complements to 100%.Preparation method：Ibid.
4th, shenqinmycin and phosethyl-Al are compounded
Embodiment 40：20.2% shenqinmycin phosethyl-Al wettable powder (1:100)
Component:Shenqinmycin 0.2%, phosethyl-Al 20%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 10%, kaolin complements to 100%.
Preparation method：Each component is well mixed, mechanical crushing is first passed through, then by after air-flow crushing, being well mixed, i.e., 20.2% shenqinmycin phosethyl-Al wettable powder can be obtained.
Comparative example 28:20.2% polyoxin phosethyl-Al wettable powder (1:100)
Component:Polyoxin 0.2%, phosethyl-Al 20%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 10%, kaolin complements to 100%.Preparation method：Ibid.
Embodiment 41:16.8% shenqinmycin phosethyl-Al water dispersible granules (1:55)
Component:Shenqinmycin 0.3%, phosethyl-Al 16.5%, lauryl sodium sulfate 4%, polycarboxylic acids sodium 4%, nothing Aqueous sodium persulfate 15%, talcum powder complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 16.8% shenqinmycin phosethyl-Al water dispersible granules are obtained.
Embodiment 42:25.5% shenqinmycin phosethyl-Al wettable powder (1:50)
Component:Shenqinmycin 0.5%, phosethyl-Al 25%, neopelex 5%, lignin sodium naphthalene sulfonate 8%, white carbon 10%, precipitated calcium carbonate complements to 100%.
Preparation method：Each component is well mixed, mechanical crushing is first passed through, then by after air-flow crushing, being well mixed, i.e., 25.5% shenqinmycin phosethyl-Al wettable powder can be obtained.
Comparative example 29：25.5% kasugarnycin phosethyl-Al wettable powder (1:50)
Component:Kasugarnycin 0.5%, phosethyl-Al 25%, neopelex 5%, lignin sodium naphthalene sulfonate 8%, white carbon 10%, precipitated calcium carbonate complements to 100%.Preparation method：Ibid.
Embodiment 43:35% shenqinmycin phosethyl-Al dry suspending agent (1:34)
Component:Shenqinmycin 1%, phosethyl-Al 34%, lignin sodium naphthalene sulfonate 10%, sodium lignin sulfonate 6%, in vain Carbon black 12%, organic silicon defoamer 0.4%, Sodium Benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method：1.5-2.0 times of water of preparation amount of preparation are added, each component is mixed, it is equal through high speed shear mixing It is even, then be sanded 2-3 hours by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 35% Shen piperazine mould Plain phosethyl-Al dry suspending agent.
Embodiment 44:26% shenqinmycin phosethyl-Al water dispersible granules (1:25)
Component:Shenqinmycin 1%, phosethyl-Al 25%, neopelex 4%, polycarboxylic acids sodium 4% is anhydrous Sodium sulphate 15%, talcum powder complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 26% shenqinmycin phosethyl-Al water dispersible granules are obtained.
Comparative example 30:26% jinggangmeisu phosethyl-Al water dispersible granules (1:25)
Component:Jinggangmeisu 1%, phosethyl-Al 25%, neopelex 4%, polycarboxylic acids sodium 4% is anhydrous Sodium sulphate 15%, talcum powder complements to 100%.Preparation method：Ibid.
Embodiment 45:32% shenqinmycin phosethyl-Al wettable powder (1:15)
Component:Shenqinmycin 2%, phosethyl-Al 30%, neopelex 3%, polycarboxylic acids sodium 5%, hard charcoal Black 12%, talcum powder complements to 100%.
Preparation method：Each component is well mixed, mechanical crushing is first passed through, then by after air-flow crushing, being well mixed, i.e., 32% shenqinmycin phosethyl-Al wettable powder can be obtained.
Embodiment 46：20% shenqinmycin phosethyl-Al dry suspending agent (1:1)
Component:Shenqinmycin 10%, phosethyl-Al 10%, polycarboxylic acids sodium 5%, sodium lignin sulfonate 10%, organosilicon Defoamer 0.4%, Sodium Benzoate 0.1%, white carbon 8%, kaolin complements to 100%.Soft water 150-200%.
Preparation method：1.5-2.0 times of water of preparation amount of preparation are added, each component is mixed, it is equal through high speed shear mixing It is even, then be sanded 2-3 hours by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 25% Shen piperazine mould Plain phosethyl-Al dry suspending agent.
Comparative example 31：20% tetramycin phosethyl-Al dry suspending agent (1:1)
Component:Tetramycin 10%, phosethyl-Al 10%, polycarboxylic acids sodium 5%, sodium lignin sulfonate 10%, organosilicon Defoamer 0.4%, Sodium Benzoate 0.1%, white carbon 8%, kaolin complements to 100%.Soft water 150-200%.Preparation method： Ibid.
Comparative example 32：20% kasugarnycin phosethyl-Al dry suspending agent (1:1)
Component:Kasugarnycin 10%, phosethyl-Al 10%, polycarboxylic acids sodium 5%, sodium lignin sulfonate 10%, organosilicon Defoamer 0.4%, Sodium Benzoate 0.1%, white carbon 8%, kaolin complements to 100%.Soft water 150-200%.Preparation method： Ibid.
Embodiment 47:21% shenqinmycin phosethyl-Al water dispersible granules (19:2)
Component:Shenqinmycin 19%, phosethyl-Al 2%, polycarboxylic acids sodium 4%, lauryl sodium sulfate 4%, anhydrous sulphur Sour sodium 10%, talcum powder complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 21% shenqinmycin phosethyl-Al water dispersible granules are obtained.
Embodiment 48：16% shenqinmycin phosethyl-Al suspending agent (15:1)
Component:Shenqinmycin 15%, phosethyl-Al 1%, polycarboxylic acids sodium 4%, fatty alcohol oxygen vinethene 3%, ethylene glycol 5%, XG0.35%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, it is well mixed through high speed shear, then be sanded 2-3 hours by sand mill, make to put down Equal particle diameter reaches 1-5 microns, you can 16% shenqinmycin phosethyl-Al suspending agent is obtained.
Embodiment 49:26% shenqinmycin phosethyl-Al water dispersible granules (25:1)
Component:Shenqinmycin 25%, phosethyl-Al 1%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, anhydrous sodium sulfate 6%, kaolin complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously Drying, you can 26% shenqinmycin phosethyl-Al water dispersible granules are obtained.
Comparative example 33:26% tetramycin phosethyl-Al water dispersible granules (25:1)
Component:Shenqinmycin 25%, phosethyl-Al 1%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, anhydrous sodium sulfate 6%, kaolin complements to 100%.Preparation method：Ibid.
Embodiment 50:23% shenqinmycin phosethyl-Al suspending agent (45:1)
Component:Shenqinmycin 22.5%, phosethyl-Al 0.5%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 3%, fat Alcohol oxygen vinethene 3%, ethylene glycol 5%, XG0.3%, organic silicon defoamer 0.2%, Sodium Benzoate 0.4%, soft water is complemented to 100%.
Preparation method：Each component is mixed, it is well mixed through high speed shear, then be sanded 2-3 hours by sand mill, make to put down Equal particle diameter reaches 1-5 microns, you can 23% shenqinmycin phosethyl-Al suspending agent is obtained.
Embodiment 51:20.4% shenqinmycin phosethyl-Al dry suspending agent (50:1)
Component:Shenqinmycin 20%, phosethyl-Al 0.4%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 8%, Organic silicon defoamer 0.4%, Sodium Benzoate 0.1%, white carbon 6%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method：1.5-2.0 times of water of preparation amount of preparation are added, each component is mixed, it is equal through high speed shear mixing It is even, then be sanded 2-3 hours by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 20.4% Shen piperazine mould Plain phosethyl-Al dry suspending agent.
Comparative example 34：20.4% kasugarnycin phosethyl-Al dry suspending agent (50:1)
Component:Kasugarnycin 20%, phosethyl-Al 0.4%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 8%, Organic silicon defoamer 0.4%, Sodium Benzoate 0.1%, white carbon 6%, talcum powder complements to 100%.Soft water 150-200%.System Preparation Method：Ibid.
Embodiment 52:20.2% shenqinmycin phosethyl-Al wettable powder (100:1)
Component：Shenqinmycin 20%, phosethyl-Al 0.2%, neopelex 3%, naphthalene sulfonate formaldehyde contracting Compound 6%, white carbon 6%, kaolin complements to 100%.
Preparation method：Each component is well mixed, mechanical crushing is first passed through, then by after air-flow crushing, being well mixed, i.e., 20.2% shenqinmycin phosethyl-Al wettable powder can be obtained.
Comparative example 35：20.2% polyoxin phosethyl-Al wettable powder (100:1)
Component：Shenqinmycin 20%, phosethyl-Al 0.2%, neopelex 3%, naphthalene sulfonate formaldehyde contracting Compound 6%, white carbon 6%, kaolin complements to 100%.Preparation method：Ibid.
Find that cyazofamid, hymexazos, fludioxonil or phosethyl-Al in above example can be replaced mutually by experiment Generation, to form new embodiment.This is no longer carried out to repeat to enumerate, it is noted that for the ordinary skill people of the art For member, on the premise of principle of the present invention is not departed from, some improvements and modifications can also be made, these improvements and modifications Also should be regarded as protection scope of the present invention.
Cucumber downy mildew, tomato late blight are prevented and treated with pesticidal preparations obtained in above-described embodiment, comparative example to test.
1st, test process：Experiment is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination Test dosing and three concentration for the treatment of are set according to the difference of each composition respectively, comparison medicament is respectively the cyanogen of commercially available agricultural chemicals 20% frost Azole suspending agent, 1% shenqinmycin SC and blank clean water experiment.
2nd, test method：Each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after being investigated before dispenser and being prevented and treated Method is：It is classified with blank incidence and is investigated, per the sampling of cell diagonal, often clicks 10 plants, record total strain number, diseased plant number With sick series.
Cucumber downy mildew field investigation standard uses following stage division：
0 grade：It is disease-free；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 5%-10% of whole leaf area；
5 grades：Lesion area accounts for the 11%-25% of whole leaf area；
7 grades：Lesion area accounts for the 26%-50% of whole leaf area；
9 grades：Lesion area accounts for more than the 50% of whole leaf area；
Result of the test see the table below 5,6：
The shenqinmycin of table 5 (A) and cyazofamid (B) compound the prevention effect to cucumber downy mildew
The field efficacy result of table 5 shows, 1 is compared in weight：100-100：In the range of 1, shenqinmycin is compounded with cyazofamid and increased Effect effect is obvious, and particularly 1：50-50：In the range of 1, especially 1：25-25：In the range of 1, have to cucumber downy mildew excellent Prevention effect, prevention effect is better than single dose kind.
Tomato late blight field investigation standard uses following stage division：
0 grade：It is disease-free；
1 grade：There are indivedual scabs on indivedual blades；
3 grades：1/3 with the ill spot of lower blade；
5 grades：1/3-1/2 blades have scab；
7 grades：Nearly all blade has scab；
9 grades：Whole blades go rotten almost without green portion；
Other statistics, experimental techniques are ibid.
The embodiment of table 6 compares the prevention effect of tomato late blight with comparative example
Inventor has found that other antibioticses bactericide are compounded with cyazofamid, and 1 is compared in weight：100-100：In the range of 1 With certain Complex synergism.Because length is limited, do not enumerate, only with most representational kasugarnycin, many anti-mildews Element, Ningnanmycin constructs comparative example and embodiment carries out preventive effect contrast, and such as field efficacy result of table 6 shows, 1 is compared in weight： 100-100：In the range of 1, shenqinmycin with cyazofamid Complex synergism substantially, is better than other antibioticses bactericide with cyanogen frost The prevention effect of azoles compounding.Particularly 1：50-50：In the range of 1, especially 1：25-25：In the range of 1, shenqinmycin is white with cyanogen Azoles Complex synergism substantially, to the prevention effect of plant disease, is better than other antibioticses bactericide with cyazofamid compounding Prevention effect.
Root rotof flax, root rot are prevented and treated with pesticidal preparations obtained in above-described embodiment, comparative example to test.
1st, test process：Experiment is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination Test dosing and three concentration for the treatment of are set according to the difference of each composition respectively, comparison medicament is respectively that the Evil of commercially available agricultural chemicals 30% is mould Lingshui Spring agent, 1% shenqinmycin SC and blank clean water experiment.
2nd, test method：Each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after being investigated before dispenser and being prevented and treated Method is：It is classified with blank incidence and is investigated, per the sampling of cell diagonal, often clicks 10 plants, record total strain number, diseased plant number With sick series.
Using following stage division：
0 grade：Basal part of stem and master, the equal disease-free spot of fibrous root；
1 grade：There is a small amount of scab on basal part of stem and main root, lesion area is accounted for below a quarter of stem and the root gross area；
3 grades：Have more scab on basal part of stem and main root, lesion area account for stem and the root gross area a quarter and two/ One；
5 grades：Have more scab on basal part of stem and main root, lesion area account for stem and the root gross area 1/2nd and four/ Three；
7 grades：In flakes, but root system is not dead for scab on basal part of stem and main root；
9 grades：Root system necrosis, above-ground plant parts is wilted close to dead or death；
Result of the test see the table below 7,8：
(A) are He hymexazo (B) compounds the prevention effect to root rotof flax to the shenqinmycin of table 7
The field efficacy result of table 5 shows, 1 is compared in weight：100-100：In the range of 1, shenqinmycin Yu hymexazos compounding increases Effect effect is obvious, and particularly 1：50-50：In the range of 1, especially 1：25-25：In the range of 1, have to root rotof flax excellent Prevention effect, prevention effect is better than single dose kind.
The embodiment of table 8 compares the prevention effect of root rot with comparative example
Inventor has found that other antibioticses bactericide Yu hymexazos are compounded, and 1 is compared in weight：100-100：In the range of 1 With certain Complex synergism.Because length is limited, do not enumerate, it is only rather mould with most representational polyoxin, Chinese parasol tree Element constructs comparative example and carries out preventive effect contrast with embodiment, and such as field efficacy result of table 8 shows, 1 is compared in weight：100-100：1 In the range of, shenqinmycin Yu hymexazos Complex synergism substantially, is better than other antibioticses bactericide Yu hymexazos compounding Prevention effect.Particularly 1：50-50：In the range of 1, especially 1：25-25：In the range of 1, shenqinmycin Yu hymexazos compounding increases Effect effect substantially, to the prevention effect of plant disease, is better than the prevention effect of other antibioticses bactericide Yu hymexazos compounding.
Grey mould fruit rot of strawberry, mango anthracnose are prevented and treated with pesticidal preparations obtained in above-described embodiment, comparative example to test.
1st, test process：Experiment is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination Test dosing and three concentration for the treatment of are set according to the difference of each composition respectively, comparison medicament is respectively that commercially available agricultural chemicals 25g/l is coughed up Bacterium nitrile wettable powder, 1% shenqinmycin SC and blank clean water experiment.
2nd, test method：Each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after being investigated before dispenser and being prevented and treated Method is：It is classified with blank incidence and is investigated, per the sampling of cell diagonal, often clicks 10 plants, record total strain number, diseased plant number With sick series.
Using following stage division：
0 grade：It is disease-free；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%-15% of whole leaf area；
5 grades：Lesion area accounts for the 16%-25% of whole leaf area；
7 grades：Lesion area accounts for below the 26%-50% of whole leaf area；
9 grades：Lesion area accounts for more than the 50% of whole leaf area；
Result of the test see the table below 9,10：
The shenqinmycin of table 9 (A) and fludioxonil (B) compound the prevention effect to grey mould fruit rot of strawberry
The field efficacy result of table 9 shows, 1 is compared in weight：100-100：In the range of 1, shenqinmycin is compounded with fludioxonil and increased Effect effect is obvious, and particularly 1：50-50：In the range of 1, especially 1：25-25：In the range of 1, have to grey mould fruit rot of strawberry excellent Prevention effect, prevention effect is better than single dose kind.
The embodiment of table 10 compares the prevention effect of mango anthracnose with comparative example
Inventor has found that other antibioticses bactericide are compounded with fludioxonil, and 1 is compared in weight：100-100：In the range of 1 With certain Complex synergism.Because length is limited, do not enumerate, only with most representational kasugarnycin, many anti-mildews Element, Ningnanmycin, jinggangmeisu, tetramycin construct comparative example and embodiment carries out preventive effect contrast, such as field efficacy of table 10 Result shows, 1 is compared in weight：100-100：In the range of 1, shenqinmycin substantially, is better than other with fludioxonil Complex synergism The prevention effect that antibioticses bactericide is compounded with fludioxonil.Particularly 1：50-50：In the range of 1, especially 1：25-25：1 In the range of, shenqinmycin substantially, to the prevention effect of plant disease, is better than other antibioticses with fludioxonil Complex synergism The prevention effect that bactericide is compounded with fludioxonil.
Chinese cabbage downy mildew, tobacco black shank are prevented and treated with pesticidal preparations obtained in above-described embodiment, comparative example to test.
1st, test process：Experiment is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination Test dosing and three concentration for the treatment of are set according to the difference of each composition respectively, comparison medicament is respectively the second of commercially available agricultural chemicals 40% 3 Phosphonic acids aluminium wettable powder, 1% shenqinmycin SC and blank clean water experiment.
2nd, test method：Each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after being investigated before dispenser and being prevented and treated Method is：It is classified with blank incidence and is investigated, per the sampling of cell diagonal, often clicks 10 plants, record total strain number, diseased plant number With sick series.
Using following stage division：
0 grade：It is disease-free；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 5%-10% of whole leaf area；
5 grades：Lesion area accounts for the 11%-25% of whole leaf area；
7 grades：Lesion area accounts for the 26%-50% of whole leaf area；
9 grades：Lesion area accounts for more than the 50% of whole leaf area；
Result of the test see the table below 11,12：
The shenqinmycin of table 11 (A) and phosethyl-Al (B) compound the prevention effect to Chinese cabbage downy mildew
The field efficacy result of table 11 shows, 1 is compared in weight：100-100：In the range of 1, shenqinmycin is answered with phosethyl-Al With synergistic effect substantially, particularly 1：50-50：In the range of 1, especially 1：25-25：In the range of 1, have to Chinese cabbage downy mildew excellent Good prevention effect, prevention effect is better than single dose kind.
Tobacco black shank investigates standard：
0 grade：Complete stool is disease-free；
1 grade：Stem's scab is no more than 1/2nd of stem girth, or the lower blade of half one is slightly wilting, or bottom minority leaf There is scab in piece；
3 grades：Stem's scab is more than 1/2nd of stem girth, or the blade of half one is slightly wilting；
5 grades：Stem's scab around stem girth, or 2/3rds wilting with blade；
7 grades：Diseased plant whole blade is wilting or withered.
The embodiment of table 12 compares the prevention effect of tobacco black shank with comparative example
Inventor has found that other antibioticses bactericide are compounded with phosethyl-Al, and 1 is compared in weight：100-100：1 scope Inside also there is certain Complex synergism.Because length is limited, do not enumerate, only with most representational kasugarnycin, many Antimycin, jinggangmeisu, tetramycin construct comparative example and embodiment carries out preventive effect contrast, such as the field efficacy result table of table 12 It is bright, compare 1 in weight：100-100：In the range of 1, shenqinmycin with phosethyl-Al Complex synergism substantially, is better than other and resists The prevention effect that raw element series bactericidal agent is compounded with phosethyl-Al.Particularly 1：50-50：In the range of 1, especially 1：25-25： In the range of 1, shenqinmycin with phosethyl-Al Complex synergism substantially, to the prevention effect of plant disease, is better than other and resists The prevention effect that raw element series bactericidal agent is compounded with phosethyl-Al.
Inventor finds shenqinmycin and cyazofamid, Shen shenqinmycin Yu hymexazo, shenqinmycin and fludioxonil, Shen piperazine are mould Element has obvious synergistic effect to plant disease with three second aluminum phosphates compounding.In identical proportion 1:100-100：In 1, Particularly 1:50-50:In the range of 1, especially 1：25-25：In the range of 1, shenqinmycin and cyazofamid, shenqinmycin are found Yu hymexazo, shenqinmycin and fludioxonil, shenqinmycin with three second aluminum phosphates compounding also with obvious synergistic effect.Due to a piece It is limited, it is not repeated.
The above is the preferred embodiment of the present invention, it is noted that for those skilled in the art For, on the premise of principle of the present invention is not departed from, some improvements and modifications can also be made, these improvements and modifications Should be regarded as protection scope of the present invention.

Claims (10)

1. a kind of bactericidal composition, it is characterised in that contain active ingredient shenqinmycin and active bactericide, the shenqinmycin It is 1 with the weight ratio of active bactericide：100~100：1.
2. bactericidal composition according to claim 1, it is characterised in that the weight of the shenqinmycin and active bactericide Than being 1：50~50：1.
3. bactericidal composition according to claim 2, it is characterised in that the weight of the shenqinmycin and active bactericide Than being 1：25~25：1.
4. according to any described bactericidal composition in claims 1 to 3, it is characterised in that the active bactericide is cyanogen frost Azoles, fludioxonil or phosethyl-Al.
5. according to any described bactericidal composition in Claims 1-4, it is characterised in that also contain in the bactericidal composition There is pesticidal preparations auxiliary element, to be made the formulation for being adapted to agriculturally use.
6. bactericidal composition according to claim 5, it is characterised in that the pesticidal preparations auxiliary element is solvent, breast One or more in agent, dispersant, stabilizer, antifreezing agent or wetting agent.
7. bactericidal composition according to claim 5, it is characterised in that the formulation of the bactericidal composition be suspending agent, Dry suspending agent, water dispersible granules, wettable powder or seed treatment.
8. application of any described bactericidal composition of claim 1 to 7 in the pesticidal preparations for preparing controlling plant diseases.
9. the application of bactericidal composition according to claim 8, it is characterised in that prevent and treat rice sheath blight disease, rice in preparation Seasonal febrile diseases, false smut, wheat rust, powdery mildew, root rotof flax, wheat sharp eyespot, root rot, sclerotinia rot of colza, corn Application in head smut, capsicum wilt, the pesticidal preparations of cucurbits fusarium wilt.
10. the method that the bactericidal composition described in claim 1 to 7 is applied to controlling disease, it is characterised in that active principle Shen Piperazine mycin and active bactericide are applied with above-mentioned weight ratio, it applies after mixing in proportion, be administered simultaneously in proportion or by than Example is applied successively.
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