CN104957147A - Bactericidal composition and application thereof - Google Patents

Bactericidal composition and application thereof Download PDF

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Publication number
CN104957147A
CN104957147A CN201510319355.1A CN201510319355A CN104957147A CN 104957147 A CN104957147 A CN 104957147A CN 201510319355 A CN201510319355 A CN 201510319355A CN 104957147 A CN104957147 A CN 104957147A
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shen piperazine
piperazine mycin
bactericidal composition
component
fludioxonil
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CN104957147B (en
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古越
李翔
陈熙
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Sichuan Lier Crop Science Co Ltd
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Sichuan Lier Crop Science Co Ltd
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Priority to CN201710043527.6A priority Critical patent/CN106818769B/en
Priority to CN201510319355.1A priority patent/CN104957147B/en
Priority to CN201610630815.7A priority patent/CN106234408B/en
Priority to CN201910096091.6A priority patent/CN111096325B/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Relating to the technical field of pesticide compounding, the invention discloses a bactericidal composition and application thereof. The effective component of the bactericidal composition contains phenazine-1-carboxylic acid A and the remaining bactericide B, wherein the rest of the remaining bactericide B is selected from cyazofamid of sulfanilamide imidazole bactericides, hymexazol of oxazole bactericides, fludioxonil of pyrrole bactericides, or phosethyl-Al of phosethyl-Al organophosphorus bactericides, and the weight ratio of A to B is 1:100-100:1. Compounding of the effective component A and B has an obvious synergistic effect, and can effectively reduce the dosage of each single agent. The bactericidal composition has good effect on expansion of the bacteriocidal spectrum and delaying of plant resistance to drugs, also can prolong the lasting period, and has obvious promotion value. The composition can be added with other pesticide allowable auxiliary ingredients to be processed into any dosage form. The bactericidal composition can effectively control of sheath blight, rice blast, Ustilaginoidea virens, rust disease, powdery mildew, root rot, Sclerotinia sclerotiorum, smut, blight and the like.

Description

Bactericidal composition and application thereof
Technical field
The present invention relates to a kind of bactericidal composition and application thereof, refer to a kind of bactericidal composition and the application thereof that contain active ingredient Shen piperazine mycin and active bactericide especially, belong to technical field of pesticide combination.
Background technology
In agricultural production, can crops healthy growth, except affecting by worm, crop smothering, also very important to the control of disease.Along with reinforcement and the sustainable development implementation of environmental protection ideas, the inexorable trend efficient, low toxicity, high activity, low-residual have become Agrochemicals.But, even if efficient germicide, be used alone for a long time, also easily make disease develop immunity to drugs, cause the problems such as dosage increasing, preventive effect reduction, lasting period shortening, be unfavorable for environment sustainable development.Therefore, how Scientific Usage of Drugs, reduces the consumption of chemical pesticide, improves drug effect, becomes the problem that plant protection art is badly in need of solving.
Cyazofamid (CyazofaMid) goes out also known as match seat, the chloro-2-cyano group of 4--N, N-dimethyl-5-p-methylphenyl imidazoles-1-sulfonamide, cyanogen azoles flusulfamide, section are good, belongs to sulphaguanidine azoles fungicide.Cyazofamid is mitochondrial respiratory inhibitor, all effect is had to all vegetative stages of oomycetes, with other bactericide without cross tolerance, there is certain interior suction and therapeutic activity, lasting period is long, resistance of rainwater washing against, to crop, the mankind, Environmental security, but long-term single use, can make germ develop immunity to drugs to it unavoidably.
Hymexazo (hymexazol), also known as hydroxyisoxazole, dislikes mould spirit, and molecular formula is C4H5NO2, chemical name: 3-hydroxy-5-methyl-isoxazole.Hymexazo is systemic fungicide, is again a kind of soil disinfectant simultaneously.The iron of this medicine in soil, aluminium ion are combined, and suppress the germination of spore.Dislike mould spirit by the root absorption of plant and to move in root system, produce two kinds of glucosides, crop is improved to the effect of physiologically active, thus can promote plant strain growth at plant intracellular metabolite, the divide increase of evil, root hair and the activity of root of root improve.To be broad-spectrum germicide to the plant disease that multiple disease fungus causes have hymexazo higher prevents and treats result, but long-term single use, germ can be made unavoidably to develop immunity to drugs to it.
Fludioxonil (fludioxonil); also known as fluorine fludioxonil, cough up bacterium ester, 4-(2; 2-bis-fluoro-1; 3-benzo dioxy-4-base) pyrroles-3-nitrile, the bacterium that do not fall dislike, suitable happy time; fludioxonil is new pyrrole class non-internal-absorting wide-spectrum bactericide; its mechanism of action is the transferase by suppressing thalline glucose phosphorylated relevant, and the mycelial growth of energy Antifungi, causes germ dead.To microbial diseases of cause of disease such as Alternaria, Ascochyta, aspergillus, Fusarium, Helminthosporium, Rhizoctonia and Penicilliums, there is well protection and therapeutic action, for preventing and treating snow rotten Fusariumsp, wheat black rot, rhizoctonia solani etc.
Phosethyl-Al (phosethyl-Al); also known as Yimeiling, the spirit of epidemic disease frost, aliette, mould epidemic disease only, Methylpartricin Sodium Laurylsulfate, mould spirit, three second aluminium phosphates, fosetyl aluminium; be that a kind of organic phosphates is efficient, wide spectrum, interior absorption low toxicity bactericide, there is treatment and protective effect.It can upper and lower Bidirectional Conduction in plant corpus.This aqueous solubility is good, and interior sorptivety permeability is strong, and the lasting period is longer, use safety.
Shen piperazine mycin, its structural formula is as shown in (I), Shen piperazine mycin is a kind of antibiotic secreted through biological culture by pseudomonas fluorescens M18, has wide spectrum Suppressing phytopathogens and the bactericide of the dual-use function of Promoting plant growth effect simultaneously, has wide spectrum, efficiently feature.
Structural formula (I) Shen piperazine mycin
Frequent use containing single-activity component bactericide, cause most germs to create pesticide resistance, preventive effect is unsatisfactory.Therefore, be badly in need of the sterilised products that a kind of novel product substitutes single-activity component, increase the resistance risk that drug effect delays disease, reduce economic loss.
Summary of the invention
It is reasonable that the technical problem to be solved in the present invention is to provide a kind of component, and synergistic effect is remarkable, and bactericidal effect is good, and drug cost is low, not easily develops immunity to drugs, to the bactericidal composition of crop safety and application thereof.
For solving the problems of the technologies described above, the invention provides technical scheme as follows:
Bactericidal composition of the present invention, its active ingredient is Shen piperazine mycin A and active bactericide B, and the weight ratio of Shen piperazine mycin A and active bactericide B is 1:100 ~ 100:1, and being preferably weight ratio is 1:50 ~ 50:1, and more preferably weight ratio is 1:25 ~ 25:1.
In bactericidal composition of the present invention, described active bactericide can be: any one in the cyazofamid of sulphaguanidine azoles fungicide, the hymexazo of oxazole series bactericidal agent, the fludioxonil of pyroles bactericide or the phosethyl-Al of organophosphorus fungicide etc.
Pesticidal preparations auxiliary element can also be contained, to make the formulation being applicable to agriculturally using in bactericidal composition of the present invention.Described pesticidal preparations auxiliary element is one or more in solvent, emulsifier, dispersant, stabilizing agent, antifreezing agent or wetting agent.
Bactericidal composition of the present invention can be prepared into any one formulation of applicable agricultural use by known method, reasonable formulation is dry suspending agent (DF), wetting powder (WP), water dispersible granules (WDG), suspending agent (SC), seed treatment or suspension seed-coating agent etc.
A wetting powder (WP) is can homodisperse solid powder preparation in water.Wetting powder of the present invention except adding as white carbon in former medicine, precipitated calcium carbonate, potter's clay, diatomite, outside the thinners such as attapulgite or inert substance, also have ionic or nonionic surface active agent (wetting agent, dispersant), wetting agent such as, alkylbenzenesulfonate (DBS-Na), alkylnaphthalene sulfonate (pulling open powder), alkylphenol-polyethenoxy base ether sulfate, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, aliphatic alcohol sulfate, fatty alcohol ethylene oxide adduct sulfonate, alkyl amide taurate, aliphatic alcohol polyethenoxy base ether (JFC), content 0.1 ~ 30%, dispersant is as naphthalene sulfonate, naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), lignin and derivative sulfonate (M-9, POLYFON etc.) thereof, polyoxyethylene poly-oxygen propylene aether block copolymer, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate (SOPA), PVA, CMC content 0.1 ~ 30%, also containing one or more materials among penetrating agent JFC, azone, thiophene ketone, penetrant t, content 0.3 ~ 30%, other auxiliary agents such as stabilizing agent, foam inhibitor, anticaking agent can also be contained.
B water dispersible granules (WDG) very fast disintegration in water, dispersion can form the granule of higher suspension dispersion.By active component, the key element such as auxiliary agent and the carrier compositions such as wetting agent, dispersant, interleaving agent, disintegrant, stabilizing agent, adhesive.Its auxiliary agent and carrier, and the compound method of precursor (preformed objects before granulation) is analogous to wetting powder and suspending agent substantially.
C suspending agent (SC) is not water soluble solid agricultural chemicals or the dispersion of immiscible liquids agricultural chemicals in water or oil.Obtained through soybean dietary fiber by active component, auxiliary agent (wetting dispersing agent, thickener, stabilizing agent, pH adjusting agent, defoamer, antifreezing agent).Wetting dispersing agent can be naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), lignin and derivative sulfonate (M-9, POLYFON etc.) thereof, alkylphenol-polyethenoxy base ether sulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, one or more in alkylphenol-polyethenoxy base phosphate, carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), condensed phosphate etc., content 0.1 ~ 12%; Thickener and stabilizing agent can be one or more in xanthan gum (XG), polyvinyl alcohol (PVA), Magnesiumaluminumsilicate etc., content 0.05 ~ 0.5%; PH adjusting agent and defoamer can optionally add in right amount.
D dry suspending agent (Dry Flowable, be called for short DF), be can very fast disintegration in water, dispersion formation higher suspension dispersion granule.Preparation method: the preparation obtained by the dehydration of suspending agent.Its auxiliary agent and carrier, and the compound method of precursor (preformed objects before dehydrating) is consistent with suspending agent.
Bactericidal composition of the present invention is applicable to the pesticidal preparations preparing controlling plant diseases.Be particularly useful for making the pesticidal preparations of the water prevention rice sheath blight, rice blast, false smut, wheat rust, powdery mildew, root rotof flax, wheat sharp eyespot, rice sheath blight disease, root rot, sclerotinia rot of colza, maize head smut, capsicum wilt, cucurbits fusarium wilt etc.
Bactericidal composition of the present invention can finished product preparation form provide, namely in bactericidal composition, each material mixes, or the composition of bactericidal composition also can provide with unit dose form, directly directly mix in bucket or tank before using, be then diluted to required concentration.
Bactericidal composition of the present invention is applied to the method for controlling disease, active principle A and B component are used with the weight ratio of 1:100-100:1, further dispenser is than being 1:50-50:1, further dispenser is than for 1:50-50:1, and it is used after can mixing according to the above ratio, use in proportion simultaneously or use successively in proportion.Concrete method of application can be powder injection process, spray-on process, smog method, fumigating system and seed treatment and soil treatment, ultra low volume spraying, mixes fertilizer, spreads fertilizer over the fields, fills with root.
Compared with prior art, beneficial effect of the present invention is:
1, compared with single dose, composite have obvious synergistic function, improves control efficiency;
2, composition is made up of the active ingredient that two kinds of mechanism of action are different, and bactericidal effect is good, is conducive to overcoming and delay the drug-fast generation of germ;
3, medicament mixture decreases dosage, thus reduces cost and alleviate the pollution to environment.
Instant component is reasonable, and treatment adds protective effect, and drug cost is low, and its activity and bactericidal effect are not the simple superposition of each composition activity, but has significant synergistic effect, good to crop safety, meets the security requirement of pesticidal preparations.
Embodiment
For making the technical problem to be solved in the present invention, technical scheme and advantage clearly, will be described in detail with specific embodiment below, but the present invention is limited to absolutely not these examples.The following stated is only the good embodiment of the present invention, only in order to explain the present invention, therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
The combination of active principles of different agricultural chemicals is made agricultural chemicals, is current development novel pesticide and the one of preventing and treating agriculturally resistance germ effectively and efficiently mode.After the pestsides synthesis of different cultivars, usually show a large amount of three kinds of type of action: summation action, synergistic effect and antagonism.Compound synergic is well filled a prescription, and can significantly improve actual control efficiency, and reducing the usage amount of agricultural chemicals, thus contribute to delaying the drug-fast generation speed of germ, is the important means of integrated control disease.
The present composition with Shen piperazine mycin (A) for a kind of active ingredient, active bactericide (B) is another active ingredient, combine between them, to plant disease, there is obvious synergistic function, and being not only the simple superposition of two kinds of pharmacy effects, this can clearly recognize in virulence test result indoor.
In order to prevent and treat the tomato late blight in agricultural production, inventor carries out composite synergy research with the cyazofamid of Shen piperazine mycin and sulphaguanidine azoles fungicide, and concrete grammar is:
Test adopts tomato late blight to be tested object.
Former medicine is mixed with the test medicine of needs, test method (blade method) is with reference to " People's Republic of China's agricultural industry criteria ".Experiment is cultivated, after sporangium to be generated, with spore under aseptic washing on suitable medium with pathogen, filter with double gauze, make sporangia suspension, under being placed in 4 DEG C of low temperature, dark processing 0.5h-3h, tries it and discharges zoospore, and regulates spore concentration to 1*10 5individual spore/L, for subsequent use; Or directly prepare 110 5individual sporangium/ml, suspension is as inoculum.Former medicine is mixed with the test medicine of needs, medicament is evenly sprayed in vacuum side of blade, after liquid natural air drying, by each process vacuum side of blade upward, be emitted in moisture preservation box by after marks for treatment.Often process is no less than 10 leaves, repeats for 4 times, and establishes the process only containing solvent and surfactant not containing active ingredient to do blank.With sporangium or zoospore suspension spray inoculation.After protectiveness test is generally chemicals treatment, about 24h inoculates; Curative tests generally 24h inoculation before chemicals treatment.The continuous illumination in every day after inoculation/dark each 12H replaces, temperature is 18 DEG C.And after inoculation, in 24H, keeping blade face to have moisture film, later relative moisture is cultivate 7 days under the condition of more than 90%.When the sick leaf rate of blank reaches more than 50%, classification investigation manages incidence everywhere.Stage division is: 0 grade: do not fall ill; 1 grade: blade only has a small amount of little scab, scab accounts for leaf area less than 10%; 3 grades: on blade, scab accounts for leaf area 10%-25%; 5 grades: on blade, scab accounts for leaf area 25%-50%; 7 grades: on blade, scab accounts for leaf area more than 50%; 9 grades: full leaf morbidity is withered.
According to blank incidence, investigate each process disease index, calculate preventive effect.By the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC50 value of each medicament, calculate the co-toxicity coefficient (CTC) of mixture with co-toxicity coefficient table, evaluate the activity of reagent agent to germ with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
Table 1 Shen piperazine mycin and the composite Toxicity Determination to tomato late blight of cyazofamid
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shen piperazine mycin (A) —— Y＝1.6974X+3.8406 4.8198 ——
Cyazofamid (B1) —— Y＝1.1207X+3.0833 51.3205 ——
A:B1 1:100 Y＝0.8133X+3.7145 38.0600 123.08
A:B1 1:50 Y＝0.8392X+3.7158 33.9062 127.29
A:B1 1:25 Y＝0.8094X+3.8296 27.9272 134.08
A:B1 1:1 Y＝0.7258X+4.4771 5.2537 167.72
A:B1 25:1 Y＝0.6666X+4.6275 3.6275 137.67
A:B1 50:1 Y＝0.7039X+4.6006 3.6931 132.88
A:B1 100:1 Y＝0.7284X+4.5749 3.83361 126.87
Toxicity Determination result table 1 shows, at weight ratio 1:100-100:1, in the scope of particularly 1:50-50:1, especially Shen piperazine mycin and cyazofamid is composite has obvious synergistic effect to plant disease in the scope of weight ratio 1:25-25:1, composite synergistic effect is more obvious, and control efficiency is excellent and be better than single dose kind.
In order to prevent and treat the sclerotinia rot of colza in agricultural production, inventor is with Shen piperazine mycin with oxazole series bactericidal agent hymexazo carries out composite synergy research, and concrete grammar is:
Test adopts sclerotinia rot of colza to be tested object.
Former medicine is mixed with the test medicine of needs, with reference to mycelial growth rate method, the preparation of bacterial classification: pour about 10mLPDA medium in sterilized medium into.Aseptically, get a mycelia block from the strain inclined plane of Sclerotinia sclerotiorum, be placed in the middle of culture dish.Continuous culture several generations, then before the test 3d, inoculation once, cultivate under 24 ~ 26 DEG C of conditions, for subsequent use.The making of toxic flat board, after first being dissolved by the PDA medium prepared, places cooling.Aseptically, added by liquid 1mL in sterilized culture dish, then add with pipette, extract 9mL medium in the culture dish of existing liquid, rocking gently shakes up (left 3 circles, right 3 circles), and it is dull and stereotyped uniformly to make thin and thick.Connect bacterium: off-the-shelf pathogen card punch is made bacterium cake, then move into transfer needle picking bacterium cake on the pastille flat board solidified, mycelia one faces down.Mark, puts in 24 ~ 26 DEG C of incubators and cultivates.Treat that control group colony diameter grows to about 6cm, right-angled intersection method investigates other data processed and record.
According to blank incidence, record lesion diameter, calculate the percentage that each process suppresses lesions grow, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC50 value of each medicament, calculate the co-toxicity coefficient (CTC) of mixture with co-toxicity coefficient table, evaluate the activity of reagent agent to germ with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
Table 2 Shen piperazine mycin is with the composite Toxicity Determination to sclerotinia rot of colza of hymexazo
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shen piperazine mycin (A) —— Y＝1.8279X+2.4389 25.1847 ——
Hymexazo (B2) —— Y＝1.9057X+1.1447 105.455 ——
A：B2 1:100 Y＝1.7340X+1.944 58.0668 176.05
A：B2 1:50 Y＝1.5407X+2.3249 54.4844 182。17
A：B2 1:25 Y＝1.4931X+2.4716 49.3597 190.34
A：B2 1:1 Y＝1.2617X+3.4027 18.4523 220.34
A：B2 25:1 Y＝1.3371X+3.3476 17.2138 150.72
A：B2 50:1 Y＝1.4700X+3.1080 19.3661 132.01
A：B2 100:1 Y＝1.3654X+3.2150 20.2906 125.07
Toxicity Determination result table 2 shows, at weight ratio 1:100-100:1, in the scope of particularly 1:50-50:1, especially in the scope of weight ratio 1:25-25:1, piperazine mycin in Shen has obvious synergistic effect Yu hymexazo is composite to plant disease, and control efficiency is excellent and be better than single dose kind.
In order to prevent and treat the wheat sharp eyespot in agricultural production, inventor carries out composite synergy research with the fludioxonil of Shen piperazine mycin and pyroles bactericide, and concrete grammar is:
Test adopts wheat sharp eyespot to be tested object.
Former medicine is mixed with the test medicine of needs, test method: fungicide bioassay pattern--Broad Bean Leaves method, with reference to " People's Republic of China's agricultural industry criteria ".Choose broad bean susceptible variety potted plant, clip same section, growing way is consistent, be with petiolate blade, put in culture dish, moisturizing is for subsequent use, experimental pharmacy is adopted former medicinal water dissolved dilution, 5 mass concentrations are set, blade is fully infiltrated 5S in pre-configured good liquid, drains unnecessary liquid, after natural air drying, cultivate by moisturizing after marks for treatment, test set not containing the process of medicament as blank.Be that 5mm bacterium cake has the one side of mycelia to be inoculated in process central vane with inoculator by diameter, 30 leaves are inoculated in each process.Protectiveness test is 24h inoculation after chemicals treatment, and curative tests is 24h inoculation before chemicals treatment.Inoculation is placed on climatic cabinate or constant temperature has in illumination humidistat in fact, cultivates under the condition of temperature 26 degrees Celsius-28 degrees Celsius, relative moisture 80%-90%.According to blank incidence, with kind of calliper record each vaccination scab length and width, with length, wide mean value represents lesion diameter record lesion diameter (unit mm). according to survey data, by preventing and treating result publicity: preventive effect=(blank diameter-process diameter)/blank diameter * 100, calculate the percentage that each process suppresses lesions grow, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC50 value of each medicament, the co-toxicity coefficient (CTC) of mixture is calculated with co-toxicity coefficient table, the activity of reagent agent to germ is evaluated with this.
According to blank incidence, record lesion diameter, calculate the percentage that each process suppresses lesions grow, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC50 value of each medicament, calculate the co-toxicity coefficient (CTC) of mixture with co-toxicity coefficient table, evaluate the activity of reagent agent to germ with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
Table 3 Shen piperazine mycin and the composite Toxicity Determination to wheat sharp eyespot of fludioxonil
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shen piperazine mycin (A) —— Y＝1.9338X+2.0570 33.2586 ——
Fludioxonil (B3) —— Y＝1.9057X+2.2920 26.3637 ——
A：B3 1:100 Y＝1.2830X+3.2849 21.5031 121.62
A：B3 1:50 Y＝1.0906X+3.6234 18.2905 144.72
A：B3 1:25 Y＝0.9393X+3.8792 15.6047 170.30
A：B3 1:1 Y＝0.9717X+3.8323 15.6883 187.48
A：B3 25:1 Y＝1.4046X+3.2208 18.4823 178.15
A：B3 50:1 Y＝15701X+2.8471 23.5048 140.77
A：B3 100:1 Y＝17365X+2.5490 25.7910 128.61
Toxicity Determination result table 3 shows, at weight ratio 1:100-100:1, in the scope of particularly 1:50-50:1, especially Shen piperazine mycin and fludioxonil is composite has obvious synergistic effect to plant disease in the scope of weight ratio 1:25-25:1, control efficiency is excellent and be better than single dose kind.
In order to prevent and treat the downy mildew of lettuce in agricultural production, inventor carries out composite synergy research with the phosethyl-Al of Shen piperazine mycin and organophosphorus fungicide, and concrete grammar is:
Test adopts downy mildew of lettuce to be tested object.
Former medicine is mixed with the test medicine of needs, test method (plate blade method) is with reference to " People's Republic of China's agricultural industry criteria ".Choose lettuce susceptible variety potted plant, clip same section, growing way is consistent, and be with petiolate blade, put in culture dish, moisturizing is for subsequent use.Single dose and each Mixed Pharmacy are arranged 5 variable concentrations gradients (on the basis of preliminary experiment result, different with proportioning according to different agents combination, concentration arranges also different, and bacteriostasis rate sets in the scope of 5% ~ 95%).Liquid is evenly sprayed in vacuum side of blade, after liquid natural air drying, each process blade and blade is gone up dorsad, for subsequent use by being emitted on after marks for treatment in moisture preservation box, test set not containing the process of medicament as blank.Vacuum side of blade is inoculated in ready Fresh spores drop 10uL, every blade inoculation 4, often process is no less than 5 leaves, protectiveness test is inoculation in 24 hours after chemicals treatment, curative tests is in chemicals treatment inoculation in first 24 hours, then cover ware lid, being placed in temperature is that 5-7d cultivated by the constant temperature and humidity incubator of 17 ~ 22 DEG C of relative moisture more than 90%, and continuous illumination every day/night 12h alternately; According to blank incidence, record lesion diameter, calculate the percentage that each process suppresses lesions grow, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC50 value of each medicament, calculate the co-toxicity coefficient (CTC) of mixture with co-toxicity coefficient table, evaluate the activity of reagent agent to germ with this.
According to blank incidence, record lesion diameter, calculate the percentage that each process suppresses lesions grow, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC50 value of each medicament, calculate the co-toxicity coefficient (CTC) of mixture with co-toxicity coefficient table, evaluate the activity of reagent agent to germ with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
Table 4 Shen piperazine mycin and the composite Toxicity Determination to downy mildew of lettuce of phosethyl-Al
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shen piperazine mycin (A) —— Y＝1.8664X+1.9415 43.5238 ——
Phosethyl-Al (B4) —— Y＝1.8435X+1.2674 105.8708 ——
A：B4 1:100 Y＝1.7797X+1.7255 69.1631 150.93
A：B4 1:50 Y＝1.5902X+2.2177 56.1834 183.29
A：B4 1:25 Y＝1.2482X+2.8734 50.5453 198.54
A：B4 1:1 Y＝1.3733X+3.1105 23.7598 259.62
A：B4 25:1 Y＝1.3500X+3.0151 29.5324 150.79
A：B4 50:1 Y＝1.0815X+3.3718 32.0268 137.49
A：B4 100:1 Y＝0.8909X+3.6372 33.8661 129.27
Toxicity Determination result table 4 shows, at weight ratio 1:100-100:1, in the scope of particularly 1:50-50:1, especially Shen piperazine mycin and phosethyl-Al is composite has obvious synergistic effect to plant disease in the scope of weight ratio 1:25-25:1, control efficiency is excellent and be better than single dose kind.
Toxicity Determination table 1-table 4 result shows, at weight ratio 1:100-100:1, in the scope of particularly 1:50-50:1, Shen piperazine mycin and cyazofamid, Shen piperazine mycin are Yu hymexazo, Shen piperazine mycin and fludioxonil, Shen piperazine mycin and phosethyl-Al is composite has obvious synergistic effect to plant disease, and control efficiency is excellent and be better than single dose kind.Especially in the scope of weight ratio 1:25-25:1, Shen piperazine mycin and cyazofamid, Shen piperazine mycin Yu hymexazo Shen piperazine mycin and the composite synergistic effect of fludioxonil, Shen piperazine mycin and three second aluminium phosphates more obvious.
For making the technical problem to be solved in the present invention, technical scheme and advantage clearly, within the scope of weight ratio 1:100 ~ 100:1, build the embodiment that Shen piperazine mycin is composite with cyazofamid, hymexazo, fludioxonil or phosethyl-Al respectively, be described in detail, but the present invention is limited to absolutely not these examples.
In addition, inventor also found through experiments, Shen piperazine mycin respectively with the composite effect of cyazofamid, hymexazo, fludioxonil or phosethyl-Al, be better than the composite effect of other antibiotics bactericide and cyazofamid, hymexazo, fludioxonil or phosethyl-Al.In order to this problem is clearly described, within the scope of weight ratio 1:100 ~ 100:1, build other antibiotics bactericide and the composite comparative example of cyazofamid, hymexazo, fludioxonil or phosethyl-Al, be described in detail.But because length is limited, representative several comparative examples can only be enumerated.It should be pointed out that and be not only confined in limited comparative example.
Bactericidal composition of the present invention can be prepared into any one formulation of applicable agricultural use by known method, reasonable formulation is wetting powder, aqueous suspension agent, oil-suspending agent, suspended emulsion, water dispersible granules.In all formulas, percentage is all weight percentage.The processing technology of the various formulation of the present composition is prior art, can change to some extent according to different situations.
Below in conjunction with embodiment, content of the present invention is described further.
One, Shen piperazine mycin and cyazofamid composite
Embodiment 1:20.2% Shen piperazine mycin cyazofamid suspending agent (1:100)
Component: Shen piperazine mycin 0.2%, cyazofamid 20%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinethene 4%, 1,2-PD 6%, XG0.32%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 20.2% Shen piperazine mycin cyazofamid suspending agent can be obtained.
Comparative example 1:20.2% polyoxin cyazofamid suspending agent (1:100)
Component: polyoxin 0.2%, cyazofamid 20%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinethene 4%, 1,2-PD 6%, XG0.32%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.Preparation method: the same.
Embodiment 2:16.8% Shen piperazine mycin cyazofamid water dispersible granules (1:55)
Component: Shen piperazine mycin 0.3%, cyazofamid 16.5%, lignin sodium naphthalene sulfonate 6%, polycarboxylic acids sodium 3%, anhydrous sodium sulfate 10%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 16.8% Shen piperazine mycin cyazofamid water dispersible granules.
Embodiment 3:25.5% Shen piperazine mycin cyazofamid wetting powder (1:50)
Component: Shen piperazine mycin 0.5%, cyazofamid 25%, lauryl sodium sulfate 4%, sodium lignin sulfonate 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 25.5% Shen piperazine mycin cyazofamid wetting powder.
Comparative example 2:25.5% kasugarnycin cyazofamid wetting powder (1:50)
Component: kasugarnycin 0.5%, cyazofamid 25%, lauryl sodium sulfate 4%, sodium lignin sulfonate 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.Preparation method: the same.
Embodiment 4:35% Shen piperazine mycin cyazofamid dry suspending agent (1:34)
Component: Shen piperazine mycin 1%, cyazofamid 34%, lignin sodium naphthalene sulfonate 10%, sodium lignin sulfonate 8%, white carbon 10%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 35% Shen piperazine mycin cyazofamid dry suspending agent.
Embodiment 5:26% Shen piperazine mycin cyazofamid water dispersible granules (1:25)
Component: Shen piperazine mycin 1%, cyazofamid 25%, sodium naphthalene sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine mycin cyazofamid water dispersible granules.
Comparative example 3:26% Ningnanmycin cyazofamid water dispersible granules (1:25)
Component: Ningnanmycin 1%, cyazofamid 25%, sodium naphthalene sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8%, talcum powder complements to 100%.Preparation method: the same.
Embodiment 6:32% Shen piperazine mycin cyazofamid suspending agent (1:15)
Component: Shen piperazine mycin 2%, cyazofamid 30%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 4%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 16% Shen piperazine mycin cyazofamid suspending agent can be obtained.
Embodiment 7:20% Shen piperazine mycin cyazofamid wetting powder (1:1)
Component: Shen piperazine mycin 10%, cyazofamid 10%, neopelex 4%, dodecyl naphthalenesulfonate formaldehyde condensation compound 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20% Shen piperazine mycin cyazofamid wetting powder.
Comparative example 4:20% polyoxin cyazofamid wetting powder (1:1)
Component: polyoxin 10%, cyazofamid 10%, neopelex 4%, dodecyl naphthalenesulfonate formaldehyde condensation compound 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.Preparation method: the same.
Comparative example 5:20% kasugarnycin cyazofamid wetting powder (1:1)
Component: kasugarnycin 10%, cyazofamid 10%, neopelex 4%, dodecyl naphthalenesulfonate formaldehyde condensation compound 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.Preparation method: the same.
Embodiment 8:21% Shen piperazine mycin cyazofamid water dispersible granules (19:2)
Component: Shen piperazine mycin 19%, cyazofamid 2%, lauryl sodium sulfate 3%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 6%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 21% Shen piperazine mycin cyazofamid water dispersible granules.
Embodiment 9:16% Shen piperazine mycin cyazofamid dry suspending agent (15:1)
Component: Shen piperazine mycin 15%, cyazofamid 1%, lignin sodium naphthalene sulfonate 8%, sodium lignin sulfonate 8%, white carbon 6%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 16% Shen piperazine mycin cyazofamid dry suspending agent.
Embodiment 10:26% Shen piperazine mycin cyazofamid water dispersible granules (25:1)
Component: Shen piperazine mycin 25%, cyazofamid 1%, lauryl sodium sulfate 3%, lignin sodium naphthalene sulfonate 6%, anhydrous sodium sulfate 8%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine mycin cyazofamid water dispersible granules.
Comparative example 6:26% Ningnanmycin cyazofamid water dispersible granules (25:1)
Component: Ningnanmycin 25%, cyazofamid 1%, lauryl sodium sulfate 3%, lignin sodium naphthalene sulfonate 6%, anhydrous sodium sulfate 8%, kaolin complements to 100%.Preparation method: the same.
Embodiment 11:23% Shen piperazine mycin cyazofamid suspending agent (45:1)
Component: Shen piperazine mycin 22.5%, cyazofamid 0.5%, polycarboxylic acids sodium 4%, fatty alcohol oxygen vinethene 3%, ethylene glycol 5%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 23% Shen piperazine mycin cyazofamid suspending agent can be obtained.
Embodiment 12:20.4% Shen piperazine mycin cyazofamid suspending agent (50:1)
Component: Shen piperazine mycin 20%, cyazofamid 0.4%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinethene 3%, 1,2-PD 5%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 20.4% Shen piperazine mycin cyazofamid suspending agent can be obtained.
Comparative example 7:20.4% kasugarnycin cyazofamid suspending agent (50:1)
Component: kasugarnycin 20%, cyazofamid 0.4%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinethene 3%, 1,2-PD 5%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.Preparation method: the same.
Embodiment 13:20.2% Shen piperazine mycin cyazofamid wetting powder (100:1)
Component: Shen piperazine mycin 20%, cyazofamid 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 6%, talcum powder complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20.2% Shen piperazine mycin cyazofamid wetting powder.
Comparative example 8:20.2% polyoxin cyazofamid wetting powder (100:1)
Component: polyoxin 20%, cyazofamid 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 6%, talcum powder complements to 100%.Preparation method: the same.
Two, piperazine mycin in Shen is Yu hymexazo is composite
Embodiment 14:20.2% Shen piperazine Mei Su hymexazo water dispersible granules (1:100)
Component: Shen piperazine mycin 0.2% ， hymexazo 20%, lauryl sodium sulfate 3%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 20.2% Shen piperazine Mei Su hymexazo water dispersible granules.
Comparative example 9:20.2% Duo Kang Mei Su hymexazo water dispersible granules (1:100)
Component: polyoxin 0.2% ， hymexazo 20%, lauryl sodium sulfate 3%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8%, kaolin complements to 100%.Preparation method: the same.
Embodiment 15:16.8% Shen piperazine Mei Su hymexazo suspending agent (1:55)
Component: Shen piperazine mycin 0.3% ， hymexazo 16.5%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 3%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG 0.32%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 16.8% Shen piperazine Mei Su hymexazo suspending agent can be obtained.
Embodiment 16:25.5% Shen piperazine Mei Su hymexazo wetting powder (1:50)
Component: Shen piperazine mycin 0.5% ， hymexazo 25%, sodium naphthalene sulfonate 5%, lauryl sodium sulfate 3%, white carbon 10%, talcum powder complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 25.5% Shen piperazine Mei Su hymexazo wetting powder.
Comparative example 10:25.5% Chinese parasol tree peaceful Mei Su hymexazo wetting powder (1:50)
Component: tetramycin 0.5% ， hymexazo 25%, sodium naphthalene sulfonate 5%, lauryl sodium sulfate 3%, white carbon 10%, talcum powder complements to 100%.Preparation method: the same.
Embodiment 17:35% Shen piperazine Mei Su hymexazo dry suspending agent (1:34)
Component: Shen piperazine mycin 1% ， hymexazo 34%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 8%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 10%, kaolin complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 35% Shen piperazine Mei Su hymexazo dry suspending agent.
Embodiment 18:26% Shen piperazine Mei Su hymexazo water dispersible granules (1:25)
Component: Shen piperazine mycin 1% ， hymexazo 25%, neopelex 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine Mei Su hymexazo water dispersible granules.
Comparative example 11:26% Duo Kang Mei Su hymexazo water dispersible granules (1:25)
Component: polyoxin 1% ， hymexazo 25%, neopelex 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8%, talcum powder complements to 100%.Preparation method: the same.
Embodiment 19:32% Shen piperazine Mei Su hymexazo suspending agent (1:15)
Component: Shen piperazine mycin 2% ， hymexazo 30%, polycarboxylic acids sodium 4%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG 0.26%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 32% Shen piperazine Mei Su hymexazo suspending agent can be obtained.
Embodiment 20:20% Shen piperazine Mei Su hymexazo wetting powder (1:1)
Component: Shen piperazine mycin 10% ， hymexazo 10%, lignin sodium naphthalene sulfonate 6%, neopelex 3%, white carbon 10%, kaolin complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20% Shen piperazine Mei Su hymexazo wetting powder.
Comparative example 12:20% Duo Kang Mei Su hymexazo wetting powder (1:1)
Component: polyoxin 10% ， hymexazo 10%, lignin sodium naphthalene sulfonate 6%, neopelex 3%, white carbon 10%, kaolin complements to 100%.Preparation method: the same.
Embodiment 21:21% Shen piperazine Mei Su hymexazo water dispersible granules (19:2)
Component: Shen piperazine mycin 19% ， hymexazo 2%, dodecyl sodium sulfate 4%, lauryl sodium sulfate 4%, anhydrous sodium sulfate 10%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 21% Shen piperazine Mei Su hymexazo water dispersible granules.
Embodiment 22:16% Shen piperazine Mei Su hymexazo dry suspending agent (15:1)
Component: Shen piperazine mycin 15% ， hymexazo 1%, polycarboxylic acids sodium 4%, lignin sodium naphthalene sulfonate 10%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 10%, kaolin complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 16% Shen piperazine Mei Su hymexazo dry suspending agent.
Embodiment 23:26% Shen piperazine Mei Su hymexazo water dispersible granules (25:1)
Component: Shen piperazine mycin 25% ， hymexazo 1%, polycarboxylic acids sodium 4%, lauryl sodium sulfate 4%, anhydrous sodium sulfate 10%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine Mei Su hymexazo water dispersible granules.
Comparative example 13:26% Chinese parasol tree peaceful Mei Su hymexazo water dispersible granules (25:1)
Component: tetramycin 25% ， hymexazo 1%, polycarboxylic acids sodium 4%, lauryl sodium sulfate 4%, anhydrous sodium sulfate 10%, kaolin complements to 100%.Preparation method: the same.
Embodiment 24:23% Shen piperazine Mei Su hymexazo suspending agent (45:1)
Component: Shen piperazine mycin 22.5% ， hymexazo 0.5%, polycarboxylic acids sodium 3%, OPEO sodium sulfonate 3%, ethylene glycol 6%, XG 0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 23% Shen piperazine Mei Su hymexazo suspending agent can be obtained.
Embodiment 25:20.4% Shen piperazine Mei Su hymexazo dry suspending agent (50:1)
Component: Shen piperazine mycin 20% ， hymexazo 0.4%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 10%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 6%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 20.4% Shen piperazine Mei Su hymexazo dry suspending agent.
Comparative example 14:20.4% Duo Kang Mei Su hymexazo dry suspending agent (50:1)
Component: polyoxin 20% ， hymexazo 0.4%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 10%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 6%, talcum powder complements to 100%.Soft water 150-200%.Preparation method: the same.
Embodiment 26:20.2% Shen piperazine Mei Su hymexazo wetting powder (100:1)
Component: Shen piperazine mycin 20% ， hymexazo 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 8%, kaolin complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20.2% Shen piperazine Mei Su hymexazo wetting powder.
Comparative example 15:20.2% Chinese parasol tree peaceful Mei Su hymexazo wetting powder (100:1)
Component: tetramycin 20% ， hymexazo 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 8%, kaolin complements to 100%.Preparation method: the same.
Three, Shen piperazine mycin and fludioxonil composite
Embodiment 27:20.2% Shen piperazine mycin fludioxonil suspending agent (1:100)
Component: Shen piperazine mycin 0.2%, fludioxonil 20%, polycarboxylic acids sodium 3%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG 0.32%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 20.2% Shen piperazine mycin fludioxonil suspending agent can be obtained.
Comparative example 16:20.2% polyoxin fludioxonil suspending agent (1:100)
Component: polyoxin 0.2%, fludioxonil 20%, polycarboxylic acids sodium 3%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG 0.32%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.Preparation method: the same.
Embodiment 28:16.8% Shen piperazine mycin fludioxonil water dispersible granules (1:55)
Component: Shen piperazine mycin 0.3%, fludioxonil 16.5%, neopelex 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 16.8% Shen piperazine mycin fludioxonil water dispersible granules.
Comparative example 17:16.8% jinggangmeisu fludioxonil water dispersible granules (1:55)
Component: jinggangmeisu 0.3%, fludioxonil 16.5%, neopelex 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8%, talcum powder complements to 100%.Preparation method: the same.
Embodiment 29:25.5% Shen piperazine mycin fludioxonil wetting powder (1:50)
Component: Shen piperazine mycin 0.5%, fludioxonil 25%, neopelex 4%, naphthalenesulfonic acid-formaldehyde condensate 8%, white carbon 6%, precipitated calcium carbonate complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 25.5% Shen piperazine mycin fludioxonil wetting powder.
Comparative example 18:25.5% kasugarnycin fludioxonil wetting powder (1:50)
Component: kasugarnycin 0.5%, fludioxonil 25%, neopelex 4%, naphthalenesulfonic acid-formaldehyde condensate 8%, white carbon 6%, precipitated calcium carbonate complements to 100%.Preparation method: the same.
Embodiment 30:35% Shen piperazine mycin fludioxonil dry suspending agent (1:34)
Component: Shen piperazine mycin 1%, fludioxonil 34%, lignin sodium naphthalene sulfonate 10%, polycarboxylic acids sodium 4%, white carbon 5%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 35% Shen piperazine mycin fludioxonil dry suspending agent.
Embodiment 31:26% Shen piperazine mycin fludioxonil water dispersible granules (1:25)
Component: Shen piperazine mycin 1%, fludioxonil 25%, sodium naphthalene sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 10%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine mycin fludioxonil water dispersible granules.
Comparative example 19:26% Ningnanmycin fludioxonil water dispersible granules (1:25)
Component: Ningnanmycin 1%, fludioxonil 25%, sodium naphthalene sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 10%, talcum powder complements to 100%.Preparation method: the same.
Embodiment 32:32% Shen piperazine mycin fludioxonil suspending agent (1:15)
Component: Shen piperazine mycin 2%, fludioxonil 30%, polycarboxylic acids sodium 3%, OPEO sodium sulfonate 3%, ethylene glycol 6%, XG 0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 32% Shen piperazine mycin fludioxonil suspending agent can be obtained.
Comparative example 20:32% tetramycin fludioxonil suspending agent (1:15)
Component: tetramycin 2%, fludioxonil 30%, polycarboxylic acids sodium 3%, OPEO sodium sulfonate 3%, ethylene glycol 6%, XG 0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.Preparation method: the same.
Embodiment 33:20% Shen piperazine mycin fludioxonil wetting powder (1:1)
Component: Shen piperazine mycin 10%, fludioxonil 10%, polycarboxylic acids sodium 5%, lauryl sodium sulfate 3%, white carbon 8%, precipitated calcium carbonate complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20% Shen piperazine mycin fludioxonil wetting powder.
Comparative example 21:20% polyoxin fludioxonil wetting powder (1:1)
Component: polyoxin 10%, fludioxonil 10%, polycarboxylic acids sodium 5%, lauryl sodium sulfate 3%, white carbon 8%, precipitated calcium carbonate complements to 100%.Preparation method: the same.
Comparative example 22:20% kasugarnycin fludioxonil wetting powder (1:1)
Component: kasugarnycin 10%, fludioxonil 10%, polycarboxylic acids sodium 5%, lauryl sodium sulfate 3%, white carbon 8%, precipitated calcium carbonate complements to 100%.Preparation method: the same.
Embodiment 34:21% Shen piperazine mycin fludioxonil water dispersible granules (19:2)
Component: Shen piperazine mycin 19%, fludioxonil 2%, neopelex 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 10%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 21% Shen piperazine mycin fludioxonil water dispersible granules.
Embodiment 35:16% Shen piperazine mycin fludioxonil dry suspending agent (15:1)
Component: Shen piperazine mycin 15%, fludioxonil 1%, lignin sodium naphthalene sulfonate 8%, sodium lignin sulfonate 8%, white carbon 6%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 16% Shen piperazine mycin fludioxonil dry suspending agent.
Comparative example 23:16% jinggangmeisu fludioxonil dry suspending agent (15:1)
Component: jinggangmeisu 15%, fludioxonil 1%, lignin sodium naphthalene sulfonate 8%, sodium lignin sulfonate 8%, white carbon 6%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.Preparation method: the same.
Embodiment 36:26% Shen piperazine mycin fludioxonil water dispersible granules (25:1)
Component: Shen piperazine mycin 25%, fludioxonil 1%, lauryl sodium sulfate 3%, lignin sodium naphthalene sulfonate 6%, anhydrous sodium sulfate 8%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine mycin fludioxonil water dispersible granules.
Comparative example 24:26% Ningnanmycin fludioxonil water dispersible granules (25:1)
Component: Ningnanmycin 25%, fludioxonil 1%, lauryl sodium sulfate 3%, lignin sodium naphthalene sulfonate 6%, anhydrous sodium sulfate 8%, kaolin complements to 100%.Preparation method: the same.
Embodiment 37:23% Shen piperazine mycin fludioxonil suspending agent (45:1)
Component: Shen piperazine mycin 22.5%, fludioxonil 0.5%, polycarboxylic acids sodium 4%, fatty alcohol oxygen vinethene 3%, ethylene glycol 5%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 23% Shen piperazine mycin fludioxonil suspending agent can be obtained.
Embodiment 38:20.4% Shen piperazine mycin fludioxonil dry suspending agent (50:1)
Component: Shen piperazine mycin 20%, fludioxonil 0.4%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 10%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 6%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 20.4% Shen piperazine mycin fludioxonil dry suspending agent.
Comparative example 25:20.4% kasugarnycin fludioxonil dry suspending agent (50:1)
Component: kasugarnycin 20%, fludioxonil 0.4%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 10%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 6%, talcum powder complements to 100%.Soft water 150-200%.Preparation method: the same.
Embodiment 39:20.2% Shen piperazine mycin fludioxonil wetting powder (100:1)
Component: Shen piperazine mycin 20%, fludioxonil 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 6%, talcum powder complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20.2% Shen piperazine mycin fludioxonil wetting powder.
Comparative example 26:20.2% polyoxin fludioxonil wetting powder (100:1)
Component: polyoxin 20%, fludioxonil 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 6%, talcum powder complements to 100%.
Preparation method: the same.
Comparative example 27:20.2% tetramycin fludioxonil wetting powder (100:1)
Component: tetramycin 20%, fludioxonil 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 6%, talcum powder complements to 100%.Preparation method: the same.
Four, Shen piperazine mycin and phosethyl-Al composite
Embodiment 40:20.2% Shen piperazine mycin phosethyl-Al wetting powder (1:100)
Component: Shen piperazine mycin 0.2%, phosethyl-Al 20%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 10%, kaolin complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20.2% Shen piperazine mycin phosethyl-Al wetting powder.
Comparative example 28:20.2% polyoxin phosethyl-Al wetting powder (1:100)
Component: polyoxin 0.2%, phosethyl-Al 20%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 10%, kaolin complements to 100%.Preparation method: the same.
Embodiment 41:16.8% Shen piperazine mycin phosethyl-Al water dispersible granules (1:55)
Component: Shen piperazine mycin 0.3%, phosethyl-Al 16.5%, lauryl sodium sulfate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 15%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 16.8% Shen piperazine mycin phosethyl-Al water dispersible granules.
Embodiment 42:25.5% Shen piperazine mycin phosethyl-Al wetting powder (1:50)
Component: Shen piperazine mycin 0.5%, phosethyl-Al 25%, neopelex 5%, lignin sodium naphthalene sulfonate 8%, white carbon 10%, precipitated calcium carbonate complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 25.5% Shen piperazine mycin phosethyl-Al wetting powder.
Comparative example 29:25.5% kasugarnycin phosethyl-Al wetting powder (1:50)
Component: kasugarnycin 0.5%, phosethyl-Al 25%, neopelex 5%, lignin sodium naphthalene sulfonate 8%, white carbon 10%, precipitated calcium carbonate complements to 100%.Preparation method: the same.
Embodiment 43:35% Shen piperazine mycin phosethyl-Al dry suspending agent (1:34)
Component: Shen piperazine mycin 1%, phosethyl-Al 34%, lignin sodium naphthalene sulfonate 10%, sodium lignin sulfonate 6%, white carbon 12%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 35% Shen piperazine mycin phosethyl-Al dry suspending agent.
Embodiment 44:26% Shen piperazine mycin phosethyl-Al water dispersible granules (1:25)
Component: Shen piperazine mycin 1%, phosethyl-Al 25%, neopelex 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 15%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine mycin phosethyl-Al water dispersible granules.
Comparative example 30:26% jinggangmeisu phosethyl-Al water dispersible granules (1:25)
Component: jinggangmeisu 1%, phosethyl-Al 25%, neopelex 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 15%, talcum powder complements to 100%.Preparation method: the same.
Embodiment 45:32% Shen piperazine mycin phosethyl-Al wetting powder (1:15)
Component: Shen piperazine mycin 2%, phosethyl-Al 30%, neopelex 3%, polycarboxylic acids sodium 5%, white carbon 12%, talcum powder complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 32% Shen piperazine mycin phosethyl-Al wetting powder.
Embodiment 46:20% Shen piperazine mycin phosethyl-Al dry suspending agent (1:1)
Component: Shen piperazine mycin 10%, phosethyl-Al 10%, polycarboxylic acids sodium 5%, sodium lignin sulfonate 10%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 8%, kaolin complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 25% Shen piperazine mycin phosethyl-Al dry suspending agent.
Comparative example 31:20% tetramycin phosethyl-Al dry suspending agent (1:1)
Component: tetramycin 10%, phosethyl-Al 10%, polycarboxylic acids sodium 5%, sodium lignin sulfonate 10%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 8%, kaolin complements to 100%.Soft water 150-200%.Preparation method: the same.
Comparative example 32:20% kasugarnycin phosethyl-Al dry suspending agent (1:1)
Component: kasugarnycin 10%, phosethyl-Al 10%, polycarboxylic acids sodium 5%, sodium lignin sulfonate 10%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 8%, kaolin complements to 100%.Soft water 150-200%.Preparation method: the same.
Embodiment 47:21% Shen piperazine mycin phosethyl-Al water dispersible granules (19:2)
Component: Shen piperazine mycin 19%, phosethyl-Al 2%, polycarboxylic acids sodium 4%, lauryl sodium sulfate 4%, anhydrous sodium sulfate 10%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 21% Shen piperazine mycin phosethyl-Al water dispersible granules.
Embodiment 48:16% Shen piperazine mycin phosethyl-Al suspending agent (15:1)
Component: Shen piperazine mycin 15%, phosethyl-Al 1%, polycarboxylic acids sodium 4%, fatty alcohol oxygen vinethene 3%, ethylene glycol 5%, XG0.35%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 16% Shen piperazine mycin phosethyl-Al suspending agent can be obtained.
Embodiment 49:26% Shen piperazine mycin phosethyl-Al water dispersible granules (25:1)
Component: Shen piperazine mycin 25%, phosethyl-Al 1%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, anhydrous sodium sulfate 6%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine mycin phosethyl-Al water dispersible granules.
Comparative example 33:26% tetramycin phosethyl-Al water dispersible granules (25:1)
Component: Shen piperazine mycin 25%, phosethyl-Al 1%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, anhydrous sodium sulfate 6%, kaolin complements to 100%.Preparation method: the same.
Embodiment 50:23% Shen piperazine mycin phosethyl-Al suspending agent (45:1)
Component: Shen piperazine mycin 22.5%, phosethyl-Al 0.5%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 3%, fatty alcohol oxygen vinethene 3%, ethylene glycol 5%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 23% Shen piperazine mycin phosethyl-Al suspending agent can be obtained.
Embodiment 51:20.4% Shen piperazine mycin phosethyl-Al dry suspending agent (50:1)
Component: Shen piperazine mycin 20%, phosethyl-Al 0.4%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 8%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 6%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 20.4% Shen piperazine mycin phosethyl-Al dry suspending agent.
Comparative example 34:20.4% kasugarnycin phosethyl-Al dry suspending agent (50:1)
Component: kasugarnycin 20%, phosethyl-Al 0.4%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 8%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 6%, talcum powder complements to 100%.Soft water 150-200%.Preparation method: the same.
Embodiment 52:20.2% Shen piperazine mycin phosethyl-Al wetting powder (100:1)
Component: Shen piperazine mycin 20%, phosethyl-Al 0.2%, neopelex 3%, naphthalenesulfonic acid-formaldehyde condensate 6%, white carbon 6%, kaolin complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20.2% Shen piperazine mycin phosethyl-Al wetting powder.
Comparative example 35:20.2% polyoxin phosethyl-Al wetting powder (100:1)
Component: Shen piperazine mycin 20%, phosethyl-Al 0.2%, neopelex 3%, naphthalenesulfonic acid-formaldehyde condensate 6%, white carbon 6%, kaolin complements to 100%.Preparation method: the same.
Find through experiment, the cyazofamid in above embodiment, hymexazo, fludioxonil or phosethyl-Al can phase trans-substitutions, to form new embodiment.No longer carry out this repeating to enumerate, it should be pointed out that for those skilled in the art, under the prerequisite not departing from principle of the present invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
The pesticidal preparations control cucumber downy mildew obtained by above-described embodiment, comparative example, tomato late blight test.
1, test process: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This test medicine consumption establishes three concentration for the treatment of respectively according to the difference of each composition, and contrast medicament is commercially available agricultural chemicals 20% cyazofamid suspending agent, 1% Shen piperazine mycin SC and blank clean water experiment respectively.
2, test method: each plot area is 50m 2, repeat 4 times, the inspection drug effect method before dispenser after Investigation and Prevention is: with blank incidence classification investigation, every community diagonal samples, and often clicks 10 strains, records total strain number, diseased plant number and sick progression.
Cucumber downy mildew field investigation standard adopts following stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for 5%-10% of whole leaf area;
5 grades: lesion area accounts for 11%-25% of whole leaf area;
7 grades: lesion area accounts for 26%-50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area;
Result of the test sees the following form 5,6:
Table 5 Shen piperazine mycin (A) and the composite control efficiency to cucumber downy mildew of cyazofamid (B)
Table 5 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and cyazofamid Complex synergism obvious, particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, have excellent control efficiency to cucumber downy mildew, control efficiency is better than single dose kind.
Tomato late blight field investigation standard adopts following stage division:
0 grade: anosis;
1 grade: indivedual blade has indivedual scab;
3 grades: 1/3 with the ill spot of lower blade;
5 grades: 1/3-1/2 blade has scab;
7 grades: nearly all blade has scab;
9 grades: all mildew and rot almost redgreen parts of blade;
Other statistics, experimental technique are the same.
Table 6 embodiment compares with the control efficiency of comparative example to tomato late blight
Inventor find other antibiotics bactericide and cyazofamid composite, within the scope of weight ratio 1:100-100:1, also there is certain Complex synergism.Because length limit, do not enumerate, only with the representational kasugarnycin of most, polyoxin, Ningnanmycin constructs comparative example and embodiment carries out preventive effect contrast, as table 6 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and cyazofamid Complex synergism are obvious, are better than other antibiotics bactericide and the composite control efficiency of cyazofamid.Particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, Shen piperazine mycin and cyazofamid Complex synergism are obvious, to the control efficiency of plant disease, are better than other antibiotics bactericide and the composite control efficiency of cyazofamid.
Root rotof flax, root rot test is prevented and treated with the pesticidal preparations that above-described embodiment, comparative example obtain.
1, test process: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This test medicine consumption establishes three concentration for the treatment of respectively according to the difference of each composition, and contrast medicament is commercially available agricultural chemicals 30% hymexazo aqua, 1% Shen piperazine mycin SC and blank clean water experiment respectively.
2, test method: each plot area is 50m 2, repeat 4 times, the inspection drug effect method before dispenser after Investigation and Prevention is: with blank incidence classification investigation, every community diagonal samples, and often clicks 10 strains, records total strain number, diseased plant number and sick progression.
Adopt following stage division:
0 grade: basal part of stem and master, fibrous root are all without scab;
1 grade: basal part of stem and main root have a small amount of scab, lesion area accounts for less than 1/4th of stem and the root gross area;
3 grades: basal part of stem and main root have more scab, lesion area accounts for 1/4th and 1/2nd of stem and the root gross area;
5 grades: basal part of stem and main root have more scab, lesion area accounts for 1/2nd and 3/4ths of stem and the root gross area;
7 grades: on basal part of stem and main root, scab in flakes, but root system is not dead;
9 grades: root system is downright bad, above-ground plant parts is wilted close to dead or dead;
Result of the test sees the following form 7,8:
(A) is with the composite control efficiency to root rotof flax of hymexazo (B) for table 7 Shen piperazine mycin
Table 5 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin is Yu hymexazo Complex synergism is obvious, particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, have excellent control efficiency to root rotof flax, control efficiency is better than single dose kind.
Table 8 embodiment compares with the control efficiency of comparative example to root rot
Inventor finds that other antibiotics bactericide is Yu hymexazo is composite, also has certain Complex synergism within the scope of weight ratio 1:100-100:1.Because length limit, do not enumerate, only with the representational polyoxin of most, tetramycin constructs comparative example and embodiment carries out preventive effect contrast, as table 8 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin, Yu hymexazo Complex synergism is obvious, is better than other antibiotics bactericide Yu the composite control efficiency of hymexazo.Particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, Shen piperazine mycin, Yu hymexazo Complex synergism is obvious, to the control efficiency of plant disease, is better than other antibiotics bactericide Yu the composite control efficiency of hymexazo.
The pesticidal preparations control grey mould fruit rot of strawberry obtained by above-described embodiment, comparative example, mango anthracnose test.
1, test process: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This test medicine consumption establishes three concentration for the treatment of respectively according to the difference of each composition, and contrast medicament is commercially available agricultural chemicals 25g/l fludioxonil wetting powder, 1% Shen piperazine mycin SC and blank clean water experiment respectively.
2, test method: each plot area is 50m 2, repeat 4 times, the inspection drug effect method before dispenser after Investigation and Prevention is: with blank incidence classification investigation, every community diagonal samples, and often clicks 10 strains, records total strain number, diseased plant number and sick progression.
Adopt following stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for 6%-15% of whole leaf area;
5 grades: lesion area accounts for 16%-25% of whole leaf area;
7 grades: lesion area accounts for less than 26%-50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area;
Result of the test sees the following form 9,10:
Table 9 Shen piperazine mycin (A) and the composite control efficiency to grey mould fruit rot of strawberry of fludioxonil (B)
Table 9 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and fludioxonil Complex synergism obvious, particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, have excellent control efficiency to grey mould fruit rot of strawberry, control efficiency is better than single dose kind.
Table 10 embodiment compares with the control efficiency of comparative example to mango anthracnose
Inventor find other antibiotics bactericide and fludioxonil composite, within the scope of weight ratio 1:100-100:1, also there is certain Complex synergism.Because length limit, do not enumerate, only with the representational kasugarnycin of most, polyoxin, Ningnanmycin, jinggangmeisu, tetramycin constructs comparative example and embodiment carries out preventive effect contrast, as table 10 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and fludioxonil Complex synergism are obvious, are better than other antibiotics bactericide and the composite control efficiency of fludioxonil.Particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, Shen piperazine mycin and fludioxonil Complex synergism are obvious, to the control efficiency of plant disease, are better than other antibiotics bactericide and the composite control efficiency of fludioxonil.
The pesticidal preparations control Chinese cabbage downy mildew obtained by above-described embodiment, comparative example, black shank test.
1, test process: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This test medicine consumption establishes three concentration for the treatment of respectively according to the difference of each composition, and contrast medicament is commercially available agricultural chemicals 40% phosethyl-Al wetting powder, 1% Shen piperazine mycin SC and blank clean water experiment respectively.
2, test method: each plot area is 50m 2, repeat 4 times, the inspection drug effect method before dispenser after Investigation and Prevention is: with blank incidence classification investigation, every community diagonal samples, and often clicks 10 strains, records total strain number, diseased plant number and sick progression.
Adopt following stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for 5%-10% of whole leaf area;
5 grades: lesion area accounts for 11%-25% of whole leaf area;
7 grades: lesion area accounts for 26%-50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area;
Result of the test sees the following form 11,12:
Table 11 Shen piperazine mycin (A) and the composite control efficiency to Chinese cabbage downy mildew of phosethyl-Al (B)
Table 11 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and phosethyl-Al Complex synergism obvious, particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, have excellent control efficiency to Chinese cabbage downy mildew, control efficiency is better than single dose kind.
Black shank investigation standard:
0 grade: complete stool is anosis;
1 grade: stem's scab is no more than 1/2nd of stem girth, or half one lower blade is slightly wilting, or there is scab in bottom minority blade;
3 grades: stem's scab exceedes 1/2nd of stem girth, or half one blade is slightly wilting;
5 grades: stem's scab is around stem girth, or 2/3rds is wilting with blade;
7 grades: the whole blade of diseased plant is wilting or withered.
Table 12 embodiment compares with the control efficiency of comparative example to black shank
Inventor find other antibiotics bactericide and phosethyl-Al composite, within the scope of weight ratio 1:100-100:1, also there is certain Complex synergism.Because length limit, do not enumerate, only with the representational kasugarnycin of most, polyoxin, jinggangmeisu, tetramycin constructs comparative example and embodiment carries out preventive effect contrast, as table 12 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and phosethyl-Al Complex synergism are obvious, are better than other antibiotics bactericide and the composite control efficiency of phosethyl-Al.Particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, Shen piperazine mycin and phosethyl-Al Complex synergism are obvious, to the control efficiency of plant disease, are better than other antibiotics bactericide and the composite control efficiency of phosethyl-Al.
Inventor finds that Shen piperazine mycin and cyazofamid, Shen, Shen piperazine mycin are Yu hymexazo, Shen piperazine mycin and fludioxonil, Shen piperazine mycin and three second aluminium phosphates are composite has obvious synergistic effect to plant disease.In identical proportion 1:100-100:1, in the scope of particularly 1:50-50:1, in the scope of especially 1:25-25:1, find that Shen piperazine mycin and cyazofamid, Shen piperazine mycin are Yu hymexazo, Shen piperazine mycin and fludioxonil, Shen piperazine mycin and three second aluminium phosphates are composite also has obvious synergistic effect.Because length is limited, be not repeated.
The above is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from principle of the present invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.

Claims (10)

1. a bactericidal composition, is characterized in that, containing active ingredient Shen piperazine mycin and active bactericide, the weight ratio of described Shen piperazine mycin and active bactericide is 1:100 ~ 100:1.
2. bactericidal composition according to claim 1, is characterized in that, the weight ratio of described Shen piperazine mycin and active bactericide is 1:50 ~ 50:1.
3. bactericidal composition according to claim 2, is characterized in that, the weight ratio of described Shen piperazine mycin and active bactericide is 1:25 ~ 25:1.
4., according to described bactericidal composition arbitrary in claims 1 to 3, it is characterized in that, described active bactericide is cyazofamid, hymexazo, fludioxonil or phosethyl-Al.
5. according to described bactericidal composition arbitrary in Claims 1-4, it is characterized in that, also containing pesticidal preparations auxiliary element in described bactericidal composition, to make the formulation being applicable to agriculturally using.
6. bactericidal composition according to claim 5, is characterized in that, described pesticidal preparations auxiliary element is one or more in solvent, emulsifier, dispersant, stabilizing agent, antifreezing agent or wetting agent.
7. bactericidal composition according to claim 5, is characterized in that, the formulation of described bactericidal composition is suspending agent, dry suspending agent, water dispersible granules, wetting powder, seed treatment or suspension seed-coating agent.
8. the application of the arbitrary described bactericidal composition of claim 1 to 7 in the pesticidal preparations of preparation controlling plant diseases.
9. the application of bactericidal composition according to claim 8, it is characterized in that, the application in the pesticidal preparations preparing the water prevention rice sheath blight, rice blast, false smut, wheat rust, powdery mildew, root rotof flax, wheat sharp eyespot, rice sheath blight disease, root rot, sclerotinia rot of colza, maize head smut, capsicum wilt, cucurbits fusarium wilt.
10. the bactericidal composition described in claim 1 to 7 is applied to the method for controlling disease, it is characterized in that, active principle Shen piperazine mycin and active bactericide are used with above-mentioned weight ratio, and it is used after mixing in proportion, use in proportion simultaneously or use successively in proportion.
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