CN104957145B - Bactericidal composition and its application - Google Patents
Bactericidal composition and its application Download PDFInfo
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- CN104957145B CN104957145B CN201510292410.2A CN201510292410A CN104957145B CN 104957145 B CN104957145 B CN 104957145B CN 201510292410 A CN201510292410 A CN 201510292410A CN 104957145 B CN104957145 B CN 104957145B
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Abstract
The invention discloses a kind of bactericidal composition and its application, are related to technical field of pesticide combination.The effective ingredient of bactericidal composition of the present invention contains shenqinmycin A and triazole bactericidal agent B, and wherein triazole bactericidal agent B is epoxiconazole, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane or tetraconazole etc..A and B weight ratio is 1：60～60：1.Effective constituents A can effectively reduce the amount of application of each single dose with B compoundings with obvious potentiation.To expanding fungicidal spectrum and delaying plant Drug resistance to have preferably effect, moreover it is possible to extend the lasting period, with obvious promotional value.Said composition allows the auxiliary element for using to be processed into arbitrary dosage form in can adding other pesticide.The bactericidal composition can effectively prevent and treat rice sheath blight disease, rice blast, false smut, wheat rust, powdery mildew, banana freckle, Fructus Musae black disease disease or graw mold of tomato etc..
Description
Technical field
The present invention relates to a kind of bactericidal composition and its application, particularly relate to one kind with shenqinmycin and triazole bactericidal agent
Bactericidal composition and its application for effective ingredient, belongs to technical field of pesticide combination.
Background technology
In agricultural production, crops can healthy growth, in addition to being affected by worm, crop smothering, the preventing and treating to disease is also very heavy
Will.With reinforcement and the sustainable development implementation of environmental protection ideas, efficient, low toxicity, high activity, low-residual have become pesticide and have sent out
The inexorable trend of exhibition.However, even efficient germicide, long-term exclusive use, also easily makes disease develop immunity to drugs, causes to use
The problems such as dose is increased, preventive effect is reduced, the lasting period shortens, is unfavorable for environment sustainable development.Therefore, how Scientific Usage of Drugs, drop
The consumption of low chemical pesticide, improves drug effect, becomes the problem that plant protection art is badly in need of solving.
Also known as moire disease, popular name spends sufficient stalk, rotten foot pestilence, features speckle to banded sclerotial blight.It is to be fallen ill by Rhizoctonia solani Kuhn infection, exists more
Occur under high temperature, super-humid conditions.Banded sclerotial blight causes harm seriously in South Rice Region of China, is one of Major Diseases in current Rice Production.
The disease prevents Oryza sativa L. from earing, or the hollow kernels of heading are more, and grain declines again.
False smut, another name Calomelas disease, pseudo- smut, belongs to fungal disease.With pushing away for some compact water rice varieties of short stem
The raising of wide and Fertilization Level, this disease generation are more and more projected.The empty flat grain of sick fringe is dramatically increased, fall ill it is latter as can the underproduction
5%～10%.False smut mainly occurs in Rice Heading blooming stage, part grain on harm fringe, at least per fringe 1～2, seriously
Up to 20～30.
Epoxiconazole (epoxiconazole), also known as epoxy bacterium azoles, chemical name：(2RS, 3RS) -1- [3- (2 monochloro-benzenes
Base) -2,3 one epoxy -2- (4 phenyl-monofluoride base) propyl group] one 1 hydrogen, one 1,2,4 one triazole.Epoxiconazole is fluorine-containing triazole type sterilization
Agent, with wide spectrum, the lasting period is long the features such as.Belong to C-14 demethylation enzyme inhibitors in sterol biosynthesis, not only with very
Good protection, activity is treated and rooted out, and there is interior suction and preferably residual activity.
Bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane (flusilazole) also known as：Nustar, Olymp, Punch, lucky star, gram bacterium star, Qiu Fu, chemical name：
Double (4 phenyl-monofluoride base) methyl (1H-1, the support of 2,4-- azoles-l- bases methylenes) silane.Bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane be in the world first it is fluorine-containing and siliceous
, highly active triazole bactericidal agent, belong to wide spectrum systemic fungicide.With systemic activity, protection and therapeutical effect.Destruction and
The biosynthesiss of ergosterol are prevented, causes cell membrane be formed, make pathogenic bacteria dead.
Tetraconazole (tetraconazole), also known as：Piece Mike, fluorine ether azoles, chemical name：；(±) -2- (2,4- dichloros
Phenyl) -3- (1H-1,2,4- triazol-1-yls) propyl group -1,1,2,2,-tetrafluoro ethylether.Tetraconazole belongs to second filial generation triazole type
Antibacterial, fluorine-containing in molecular structure, bactericidal activity ratio is 2-3 times of the first generation, and wide sterilization spectrum, efficient, lasting period are up to 4-6
In week, with protection and therapeutical effect, and there is good Uptake and translocation performance.
Shenqinmycin, as shown in (I), shenqinmycin is secreted by pseudomonas fluorescens M18 Jing biological cultures to its structural formula
A kind of antibiotics, while with wide spectrum Suppressing phytopathogens and promote Effects on Plant Growth dual-use function antibacterial,
With wide spectrum, it is efficient the characteristics of.
Structure formula I shenqinmycin
Frequent use containing single-activity component antibacterial, causes most germs to generate Drug resistance, and preventive effect is not
It is preferable, it is necessary to continue to increase the dosage of pesticide, can be only achieved the purpose for eliminating pathogenic bacteria.Although shenqinmycin, epoxiconazole, fluorine silicon
Azoles, tetraconazole as antibacterial be it is efficient, but its single dose life-time service, easily make disease develop immunity to drugs, cause use
Dose is increased, the problem that preventive effect is reduced, the lasting period shortens, and is unfavorable for environment sustainable development.Therefore, it is badly in need of a kind of novel product
The sterilised products of single-activity component are substituted, increases the resistance risk that drug effect delays disease, reduce economic loss.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of component rationally, and significantly, bactericidal effect is good, medication for potentiation
Low cost, is not likely to produce Drug resistance, the bactericidal composition to crop safety and its application.
To solve above-mentioned technical problem, it is as follows that the present invention provides technical scheme：
The bactericidal composition of the present invention, its effective ingredient are shenqinmycin A and triazole bactericidal agent B, shenqinmycin and three
The weight ratio of azoles fungicide is 1:60～60:1, preferably weight ratio is 1：30～30：1, more preferably weight ratio is 1：15～
15：1.
In the bactericidal composition of the present invention, the triazole bactericidal agent B is fluorine-containing triazole bactericidal agent, preferably fluorine ring
Any one in azoles, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane or tetraconazole etc..
Pesticidal preparations auxiliary element can also be contained in bactericidal composition of the present invention, be adapted to agriculturally use to make
Dosage form.The pesticidal preparations auxiliary element is the one kind in solvent, emulsifying agent, dispersant, stabilizer, antifreezing agent or wetting agent
Or it is several.
The bactericidal composition of the present invention can be prepared into any one dosage form of suitable agricultural use with known method, than
Preferably dosage form is dry suspending agent (DF), wettable powder (WP), water dispersible granules (WDG), suspending agent (SC) etc..
A wettable powders (WP), being can homodisperse solid powder preparation in water.Wettable powder of the present invention except
To in active compound plus such as outside the diluent such as white carbon, precipitated calcium carbonate, potter's clay, kieselguhr, attapulgite or inert substance, also from
Subtype or nonionic surfactant (wetting agent, dispersant), wetting agent for example, alkylbenzenesulfonate (DBS-Na), alkyl
Naphthalene sulfonate (pull open powder), alkylphenol-polyethenoxy base ether sulfate, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulfate,
Aliphatic alcohol sulfate, fatty alcohol ethylene oxide adduct sulfonate, alkyl amide taurate, aliphatic alcohol polyethenoxy base ether
(JFC), content 0.1～30%；Dispersant such as naphthalene sulfonate, naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), wood
Quality and its derivant sulfonate (M-9, POLYFON etc.), polyoxyethylene poly-oxygen propylene aether block copolymer, alkyl phenol polyoxy second
Alkene ether formaldehyde condensation products sulfate (SOPA), PVA, CMC content 0.1～30%；Also containing penetrating agent JFC, azone, thiophene ketone,
One or more material among penetrant t, content 0.3～30%；Can also be containing stabilizer, foam inhibitor, anticaking agent etc.
Other auxiliary agents.
B water dispersible granules (WDG), are very fast disintegrate, dispersion to form the granule of higher suspension dispersion in water.By
The auxiliary agent and carrier etc. such as active component, wetting agent, dispersant, interleaving agent, disintegrating agent, stabilizer, adhesive key element is constituted.Which helps
Agent and carrier, and the compound method of precursor (preformed objects before pelletize) is analogous to wettable powder and suspending agent substantially.
C suspending agents (SC), are the not dispersion of water soluble solid pesticide or immiscible liquids pesticide in water or oil.By activity
Composition, auxiliary agent (wetting dispersing agent, thickening agent, stabilizer, pH adjusting agent, defoamer, antifreezing agent) Jing soybean dietary fibers are obtained.
Wetting dispersing agent can be naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), lignin and its derivant sulfonate
(M-9, POLYFON etc.), alkylphenol-polyethenoxy base ether sulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, alkyl
Phenol polyethenoxy base phosphate ester, carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP), Polyethylene Glycol (PEG), condensation phosphorus
One or more in hydrochlorate etc., content 0.1～12%；Thickening agent and stabilizer can be Xanthan gum (XG), polyvinyl alcohol
(PVA), one or more in Magnesiumaluminumsilicate etc., content 0.05～0.5%；PH adjusting agent and defoamer optionally can add in right amount
Plus.
D dry suspending agents (Dry Flowable, abbreviation DF), are very fast disintegrate, dispersion to form higher suspension dispersion in water
The granule of system.Preparation method：The preparation obtained by the dehydration of suspending agent.Its auxiliary agent and carrier, and precursor (dehydrate
Front preformed objects) compound method it is consistent with suspending agent.
Bactericidal composition of the present invention is applied to the pesticidal preparations for preparing controlling plant diseases.It is particularly useful for making
Prevent and treat rice sheath blight disease, rice blast, false smut, wheat rust, powdery mildew banana freckle, Fructus Musae black disease disease, graw mold of tomato
Etc. the pesticidal preparations of same domain disease.
Bactericidal composition of the present invention can be provided with finished product preparation form, i.e., in bactericidal composition, each material has been mixed
Close, or the composition of bactericidal composition can also be provided with unit dose form, directly directly mixed in bucket or tank using front, so
Concentration needed for being diluted to afterwards.
The method that bactericidal composition of the present invention is applied to controlling disease, active component A shenqinmycins and component B tri-
Azoles fungicide (epoxiconazole, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane, tetraconazole) is with 1:60～60:Than applying, preferred weight ratio of applying is 1 to 1 weight：
30～30：1, it is 1 more preferably to apply weight ratio：15～15：1, which can be applied after being mixed in proportion, be administered simultaneously in proportion or by
Ratio is applied successively.Concrete method of application can be at powder injection process, nebulization, smog method, fumigation and steaming method and seed treatment and soil
Reason, ultra-low volume spray, mix fertilizer, spread fertilizer over the fields, pouring root.
Compared with prior art, the beneficial effects of the present invention is：
1st, compared with single dose, compounding improves prevention effect with obvious synergistic function；
2nd, compositionss are made up of two kinds of different effective ingredient of mechanism of action, and bactericidal effect is good, is conducive to overcoming and delays
The drug-fast generation of pathogenic bacteria；
3rd, medicament mixture reduces dosage, so as to reducing cost and alleviating the pollution to environment.
Rationally, treatment adds protective effect to instant component, and drug cost is low, and its activity and bactericidal effect are not each components
The simple superposition of activity, but have significant potentiation, it is good to crop safety, meet the security requirement of pesticidal preparations.
Specific embodiment
To make the technical problem to be solved in the present invention, technical scheme and advantage clearer, specific embodiment will be used below
It is described in detail, but the present invention is limited to absolutely not these examples.Described below is only the present invention preferably embodiment, only to
The present invention is explained, therefore the restriction to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that all the present invention's
Any modification, equivalent and improvement made within spirit and principle etc., should be included within the scope of the present invention.
Therefore, the protection domain of patent of the present invention should be defined by claims.
The combination of active principles of different pesticide is made into pesticide, is to develop and develop novel pesticide at present and prevent and treat agriculturally
A kind of effective and quick way of resistance pathogenic bacteria.After the pestsides synthesis of different cultivars, a large amount of three kinds of effects classes are typically exhibited
Type：Summation action, potentiation and antagonism.The good formula of compound synergic, can significantly improve actual prevention effect, drop
The usage amount of low pesticide, so as to contribute to delaying the drug-fast generation speed of pathogenic bacteria, is the important means of integrated control disease.
The present composition with shenqinmycin (A) as a kind of effective ingredient, triazole bactericidal agent (B) for it is another effectively into
Point, triazole bactericidal agent (B) is fluorine-containing triazole bactericidal agent, preferably epoxiconazole, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane or tetraconazole.They it
Between combine there is to plant disease obvious synergistic function, and the not exclusively simple superposition of two kinds of pharmacy effects, this can
To be clearly understood that from indoor virulence test result.
In order to prevent and treat the sigatoka in agricultural production, the potentiation compounded with shenqinmycin and epoxiconazole by inventor
Study, concrete grammar is：
Test adopts sigatoka for test object.
The test medicine that active compound is configured to need, with reference to mycelial growth rate method, the preparation of strain：It is sterilized
Pour about 10mLPDA culture medium in culture medium into.Aseptically, a mycelia is taken from the strain inclined plane of sigatoka bacterium
Block, is placed in the middle of culture dish.Continuous culture several generations, then before the test 3d, in inoculation once, train under the conditions of 24～26 DEG C
Support, it is standby.The making of toxic flat board, after first dissolve the PDA culture medium for having prepared, places cooling.Aseptically, will
During medicinal liquid 1mL adds sterilized culture dish, during the culture dish of existing medicinal liquid is then added with pipette, extract 9mL culture medium,
Rocking gently shakes up (left 3 enclose, and right 3 enclose), makes the uniform flat board of thin and thick.Connect bacterium：Off-the-shelf pathogen is punched
Device makes bacteria cake, is then moved on the pastille flat board as solidified with Inoculating needle picking bacteria cake, and mycelia one faces down.Labelling, puts 24
Cultivate in～26 DEG C of incubators.Matched group colony diameter length is treated to 6cm or so, crossing method investigates the data of other process simultaneously
Record.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as potentiation；CTC≤80 show as antagonism；80 ﹤
CTC ﹤ 120 show as summation action.
The Toxicity Determination of 1 shenqinmycin of table and epoxiconazole compounding to sigatoka
| Chemicals treatment | Proportioning | Virulence regression equation | EC50(mg/L) | Co-toxicity coefficient (CTC) |
| Shenqinmycin (A) | —— | Y=3.6303+0.8267X | 45.3644 | —— |
| Epoxiconazole (B1) | —— | Y=3.9332+0.5542X | 84.1322 | —— |
| A:B1 | 1:60 | Y=4.4699+0.3359X | 37.8592 | 120.46 |
| A:B1 | 1:50 | Y=4.0943+0.5000X | 64.7725 | 123.30 |
| A:B1 | 1:30 | Y=3.9946+0.5537X | 65.4543 | 125.08 |
| A:B1 | 1:25 | Y=4.4356+0.3623X | 36.0161 | 126.62 |
| A:B1 | 1:15 | Y=3.8988+0.7061X | 36.2556 | 128.83 |
| A:B1 | 1:9 | Y=4.4576+0.3513X | 34.9919 | 130.33 |
| A:B1 | 1:1 | Y=3.9079+0.7062X | 35.1847 | 130.87 |
| A:B1 | 8:1 | Y=4.4600+0.3541X | 33.4997 | 136.61 |
| A:B1 | 15:1 | Y=4.4650+0.3487X | 34.2113 | 133.30 |
| A:B1 | 25:1 | Y=4.4673+0.3428X | 35.7983 | 127.39 |
| A:B1 | 30:1 | Y=3.8993+0.7049X | 36.4282 | 126.41 |
| A:B1 | 49:1 | Y=3.9141+0.6475X | 47.5422 | 123.98 |
| A:B1 | 60:1 | Y=4.0969+0.4933X | 67.7449 | 122.47 |
Toxicity Determination result table 1 shows, compares 1 in weight:60-60:1, particularly 1:30-30:In the range of 1, especially
Which compares 1 in weight：15-15：In the range of 1, shenqinmycin has obvious potentiation with epoxiconazole compounding to plant disease,
The synergistic effect of compounding becomes apparent from, and prevention effect is excellent and is better than single dose kind.
In order to prevent and treat the leaf spot of peanut in agricultural production, the potentiation compounded with shenqinmycin and bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane by inventor
Study, concrete grammar is：
Test adopts leaf spot of peanut for test object.
The test medicine that active compound is configured to need, with reference to mycelial growth rate method, the preparation of strain：It is sterilized
Pour about 10mLPDA culture medium in culture medium into.Aseptically, a mycelia is taken from the strain inclined plane of leaf spot of peanut pathogenic bacteria
Block, is placed in the middle of culture dish.Continuous culture several generations, then before the test 3d, in inoculation once, train under the conditions of 24～26 DEG C
Support, it is standby.The making of toxic flat board, after first dissolve the PDA culture medium for having prepared, places cooling.Aseptically, will
During medicinal liquid 1mL adds sterilized culture dish, during the culture dish of existing medicinal liquid is then added with pipette, extract 9mL culture medium,
Rocking gently shakes up (left 3 enclose, and right 3 enclose), makes the uniform flat board of thin and thick.Connect bacterium：Off-the-shelf pathogen is punched
Device makes bacteria cake, is then moved on the pastille flat board as solidified with Inoculating needle picking bacteria cake, and mycelia one faces down.Labelling, puts 24
Cultivate in～26 DEG C of incubators.Matched group colony diameter length is treated to 6cm or so, crossing method investigates the data of other process simultaneously
Record.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as potentiation；CTC≤80 show as antagonism；80 ﹤
CTC ﹤ 120 show as summation action.
The Toxicity Determination of 2 shenqinmycin of table and bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane compounding to leaf spot of peanut
| Chemicals treatment | Proportioning | Virulence regression equation | EC50(mg/L) | Co-toxicity coefficient (CTC) |
| Shenqinmycin (A) | —— | Y=3.6303+0.8267X | 45.3644 | —— |
| Bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane (B2) | —— | Y=3.9299+0.5269X | 107.3661 | —— |
| A:B2 | 1:60 | Y=4.0500+0.4897X | 87.0583 | 120.62 |
| A:B2 | 1:50 | Y=4.0483+0.4941X | 84.3429 | 123.97 |
| A:B2 | 1:30 | Y=4.0474+0.4980X | 81.8182 | 125.66 |
| A:B2 | 1:25 | Y=4.0465+0.5019X | 79.4026 | 128.43 |
| A:B2 | 1:15 | Y=4.0456+0.5066X | 76.5773 | 129.12 |
| A:B2 | 1:9 | Y=4.0455+0.5138X | 72.1023 | 130.91 |
| A:B2 | 1:1 | Y=4.0274+0.5767X | 48.5665 | 130.97 |
| A:B2 | 8:1 | Y=4.1063+0.5705X | 36.8511 | 131.06 |
| A:B2 | 15:1 | Y=4.1251+0.5647X | 35.4158 | 132.39 |
| A:B2 | 25:1 | Y=4.1245+0.5694X | 34.4837 | 134.03 |
| A:B2 | 30:1 | Y=4.1430+0.5497X | 36.2265 | 127.12 |
| A:B2 | 49:1 | Y=4.1430+0.5497X | 36.6753 | 124.66 |
| A:B2 | 60:1 | Y=4.1423+0.5438X | 37.7727 | 120.78 |
Toxicity Determination result table 2 shows, compares 1 in weight:60-60:1, particularly 1:30-30:In the range of 1, especially
Which compares 1 in weight：15-15：In the range of 1, shenqinmycin has obvious potentiation with bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane compounding to plant disease,
The synergistic effect of compounding becomes apparent from, and prevention effect is excellent and is better than single dose kind.
In order to prevent and treat the powdery mildew of cucumber in agricultural production, the increasing compounded with shenqinmycin and tetraconazole by inventor
Effect is studied, and concrete grammar is：
Test adopts powdery mildew of cucumber for test object.
The test medicine that active compound is configured to need, test method (pot-culture method) reference《People's Republic of China's agricultural row
Industry standard》.Wholeheartedly cucumber seedling is standby for the SANYE for selecting growing way consistent, and single dose and each Mixed Pharmacy are arranged 5 variable concentrations ladders
Degree (on the basis of pilot study result, combines different with proportioning according to different agents, concentration arranges also different, antibacterial
Rate is set in the range of 5%～95%).With nebulization by medicine liquid spray on standby cucumber seedling, dry naturally after it is standby, examination
Test the process without medicament is set as blank.Will be the fresh sporozoite of Powdery Mildew produced in 24h on morbidity cucumber leaves uniform
Tiltedly fall to being inoculated on the 2-3 leaf phases potted plant cucumber seedling of process, often process 3 basins.Protectiveness tests the 24h after chemicals treatment, treatment
Property test before chemicals treatment 24h inoculation, then put 28 degrees Celsius of constant temperature lighting box cultures.According to blank incidence,
Record lesion diameter, calculates each percentage rate for processing and suppressing scab growth, by the logarithm of the probit value and series concentration of suppression ratio
Linear regression analyses between value, obtain the EC50 values of each medicament, calculate the co-toxicity coefficient of mixture with co-toxicity coefficient meter
(CTC), with this evaluating activity of the reagent agent to pathogenic bacteria.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as potentiation；CTC≤80 show as antagonism；80 ﹤
CTC ﹤ 120 show as summation action.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as potentiation；CTC≤80 show as antagonism；80 ﹤
CTC ﹤ 120 show as summation action.
The Toxicity Determination of 3 shenqinmycin of table and tetraconazole compounding to powdery mildew of cucumber
| Chemicals treatment | Proportioning | Virulence regression equation | EC50(mg/L) | Co-toxicity coefficient (CTC) |
| Shenqinmycin (A) | —— | Y=3.6303+0.8267X | 45.3644 | |
| Tetraconazole (B3) | —— | Y=3.5271+0.7508X | 91.5936 | |
| A:B3 | 1:60 | Y=3.8260+0.6283X | 73.8804 | 121.9 |
| A:B3 | 1:50 | Y=3.8251+0.6299X | 73.3287 | 122.44 |
| A:B3 | 1:30 | Y=3.8246+0.6326X | 72.0938 | 123.00 |
| A:B3 | 1:25 | Y=3.8278+0.6335X | 70.8386 | 124.38 |
| A:B3 | 1:15 | Y=3.8309+0.6369X | 68.5011 | 125.65 |
| A:B3 | 1:9 | Y=3.8314+0.6325X | 70.3863 | 128.81 |
| A:B3 | 1:1 | Y=3.8709+0.6762X | 46.7667 | 129.41 |
| A:B3 | 8:1 | Y=3.8803+0.7165X | 36.7088 | 130.44 |
| A:B3 | 15:1 | Y=3.8795+0.7239X | 35.3055 | 132.18 |
| A:B3 | 25:1 | Y=3.8779+0.7313X | 34.2230 | 134.64 |
| A:B3 | 30:1 | Y=3.8807+0.7193X | 35.9815 | 127.68 |
| A:B3 | 49:1 | Y=3.8763+0.7147X | 37.3397 | 122.26 |
| A:B3 | 60:1 | Y=3.8769+0.7126X | 37.6843 | 120.92 |
Toxicity Determination result table 3 shows, compares 1 in weight:60-60:1, particularly 1:30-30:In the range of 1, especially
Which compares 1 in weight：15-15：In the range of 1, shenqinmycin there is obvious potentiation to make with tetraconazole compounding to plant disease
With the synergistic effect of compounding becomes apparent from, and prevention effect is excellent and is better than single dose kind.
Toxicity Determination result table 1- tables 3 show, compare 1 in weight:60-60:1, particularly 1:30-30:1 scope
Interior, shenqinmycin has obvious with epoxiconazole, shenqinmycin and bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane, shenqinmycin and tetraconazole compounding to plant disease
Potentiation, prevention effect is excellent and is better than single dose kind.
To make the technical problem to be solved in the present invention, technical scheme and advantage clearer, compare 1 in weight:60～60:1
In the range of, embodiment of the shenqinmycin respectively with epoxiconazole, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane or tetraconazole compounding is built, is described in detail, but
The present invention is limited to absolutely not these examples.
In addition, inventor is also found through experiments, shenqinmycin is answered with epoxiconazole, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane or tetraconazole respectively
With effect, better than other triazole bactericidal agents and the compounding effect of shenqinmycin.In order to clearly demonstrate this problem, in weight
Than 1:60～60:The comparative example of other triazole bactericidal agents and shenqinmycin compounding in the range of 1, is built, is described in detail.But
As length is limited, representative several comparative examples can only be enumerated.It should be pointed out that not being limited to limited contrast
In example.
The bactericidal composition of the present invention can be prepared into any one dosage form of suitable agricultural use with known method, than
Preferably dosage form is wettable powder, aqueous suspension agent, oil-suspending agent, suspended emulsion, water dispersible granules.Percentage ratio in all formula
It is weight percentage.The processing technique of the various dosage forms of the present composition is prior art, can be had according to different situations
Changed.
Present invention is described further with reference to embodiment.
One：Shenqinmycin is compounded with epoxiconazole
Embodiment 1：30.5% shenqinmycin epoxiconazole wettable powder (1:60)
Component:Shenqinmycin 0.5%, epoxiconazole 30%, dodecylbenzene sodium sulfonate 6%, sodium lignin sulfonate 12%, in vain
White carbon black 20%, Pulvis Talci complements to 100%.
Preparation method：By each component mix homogeneously, mechanical activation comminution is first passed through, then after comminution by gas stream, mix homogeneously, i.e.,
30.5% shenqinmycin epoxiconazole wettable powder can be obtained.
Comparative example 1:30.5% polyoxin epoxiconazole wettable powder (1:60)
Component:Polyoxin 0.5%, epoxiconazole 30%, dodecylbenzene sodium sulfonate 6%, sodium lignin sulfonate 12%, in vain
White carbon black 20%, Pulvis Talci complements to 100%.
Preparation method：Ibid.
Comparative example 2:30.5% shenqinmycin propiconazole wettable powder (1:60)
Component:Shenqinmycin 0.5%, propiconazole 30%, dodecylbenzene sodium sulfonate 6%, sodium lignin sulfonate 12%, in vain
White carbon black 20%, Pulvis Talci complements to 100%.
Preparation method：Ibid.
Embodiment 2:46% shenqinmycin epoxiconazole water dispersant (1:45)
Component:Shenqinmycin 1%, epoxiconazole 45%, polycarboxylic acids sodium 5%, dodecylbenzene sodium sulfonate 4%, white carbon
8%, Kaolin supplies 100%.
Preparation method：Each component is mixed, Jing mechanical activation comminution, then Jing comminution by gas stream, mix homogeneously, then Jing wet granulations are simultaneously
Drying, you can 46% shenqinmycin epoxiconazole water dispersant is obtained.
Comparative example 3:46% jingganmycin epoxiconazole water dispersant (1:45)
Component:Jingganmycin 1%, epoxiconazole 45%, polycarboxylic acids sodium 5%, dodecylbenzene sodium sulfonate 4%, white carbon
8%, Kaolin supplies 100%.
Preparation method：Ibid.
Embodiment 3:31% shenqinmycin epoxiconazole wettable powder (1:30)
Component:Shenqinmycin 1%, epoxiconazole 30%, dodecylbenzene sodium sulfonate 3%, polycarboxylic acids sodium 4%, white carbon
8%, Pulvis Talci complements to 100%.
Preparation method：By each component mix homogeneously, mechanical activation comminution is first passed through, then after comminution by gas stream, mix homogeneously, i.e.,
31% shenqinmycin epoxiconazole wettable powder can be obtained.
Embodiment 4:25% shenqinmycin epoxiconazole suspension concentrate (1:24)
Component:Shenqinmycin 1%, epoxiconazole 24%, fatty alcohol-polyoxyethylene ether 3%, polycarboxylic acids sodium 4%, ethylene glycol
6%, XG0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, Jing high speed shear mix homogeneously, then by sand mill sand milling 2-3 hours, make to put down
Particle diameter reaches 1-5 microns, you can 16% shenqinmycin epoxiconazole suspension concentrate is obtained.
Comparative example 4:25% shenqinmycin hexaconazole suspending agent (1:24)
Component:Shenqinmycin 1%, hexaconazole 24%, fatty alcohol-polyoxyethylene ether 3%, polycarboxylic acids sodium 4%, ethylene glycol
6%, XG0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method：Ibid.
Embodiment 5:38% shenqinmycin epoxiconazole water dispersant (1:18)
Component:Shenqinmycin 2%, epoxiconazole 36%, polycarboxylic acids sodium 6%, sodium lauryl sulphate 4%, anhydrous sodium sulfate
10%, Kaolin supplies 100%.
Preparation method：Each component is mixed, Jing mechanical activation comminution, then Jing comminution by gas stream, mix homogeneously, then Jing wet granulations are simultaneously
Drying, you can 38% shenqinmycin epoxiconazole water dispersant is obtained.
Embodiment 6:16% shenqinmycin epoxiconazole dry suspending agent (1:15)
Component:Shenqinmycin 1%, epoxiconazole 15%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 8%, organosilicon
Defoamer 0.4%, sodium benzoate 0.1%, white carbon 6%, Pulvis Talci complements to 100%.Soft water 150-200%.
Preparation method：The water of 1.5-2.0 times of preparation amount of preparation is added, each component is mixed, the mixing of Jing high speed shear is equal
It is even, then by sand mill sand milling 2-3 hours, make mean diameter reach 1-5 microns, it is mould that dehydrate can be prepared by 16% Shen piperazine
Plain epoxiconazole dry suspending agent.
Embodiment 7:20% shenqinmycin epoxiconazole water dispersant (1:1)
Component:Shenqinmycin 10%, epoxiconazole 10%, dodecylbenzene sodium sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous slufuric acid
Sodium 10%, Pulvis Talci complements to 100%.
Preparation method：Each component is mixed, Jing mechanical activation comminution, then Jing comminution by gas stream, mix homogeneously, then Jing wet granulations are simultaneously
Drying, you can 20% shenqinmycin epoxiconazole water dispersant is obtained.
Comparative example 5:20% jingganmycin epoxiconazole water dispersant (1:1)
Component:Jingganmycin 10%, epoxiconazole 10%, dodecylbenzene sodium sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous slufuric acid
Sodium 10%, Pulvis Talci complements to 100%.
Preparation method：Ibid.
Comparative example 6:20% polyoxin epoxiconazole water dispersant (1:1)
Component:Polyoxin 10%, epoxiconazole 10%, dodecylbenzene sodium sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous slufuric acid
Sodium 10%, Pulvis Talci complements to 100%.
Preparation method：Ibid.
Embodiment 8:31% shenqinmycin epoxiconazole wettable powder (30:1)
Component:Shenqinmycin 30%, epoxiconazole 1%, sodium lauryl sulphate 3%, lignin sodium naphthalene sulfonate 5%, Linesless charcoal
Black 10%, Kaolin complements to 100%.
Preparation method：By each component mix homogeneously, mechanical activation comminution is first passed through, then after comminution by gas stream, mix homogeneously, i.e.,
31% shenqinmycin epoxiconazole wettable powder can be obtained.
Comparative example 7:31% shenqinmycin propiconazole wettable powder (30:1)
Component:Shenqinmycin 30%, propiconazole 1%, sodium lauryl sulphate 3%, lignin sodium naphthalene sulfonate 5%, Linesless charcoal
Black 10%, Kaolin complements to 100%.
Preparation method：Ibid.
Embodiment 9:30.5% shenqinmycin epoxiconazole suspension concentrate (60:1)
Component：Shenqinmycin 30%, epoxiconazole 0.5%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 3%, octyl phenol polyoxy
Vinyl Ether sodium sulfonate 3%, 1,2-PD 6%, XG 0.25%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water
Complement to 100%.
Preparation method：Each component is mixed, Jing high speed shear mix homogeneously, then by sand mill sand milling 2-3 hours, make to put down
Particle diameter reaches 1-5 microns, you can 30.5% shenqinmycin epoxiconazole suspension concentrate is obtained.
Comparative example 8:30.5% shenqinmycin hexaconazole suspending agent (60:1)
Component：Shenqinmycin 30%, hexaconazole 0.5%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 3%, octyl phenol polyoxy
Vinyl Ether sodium sulfonate 3%, 1,2-PD 6%, XG 0.25%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water
Complement to 100%.
Preparation method：Ibid.
Two：Shenqinmycin is compounded with bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane
Embodiment 10：30.5% shenqinmycin bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane wettable powder (1:60)
Component:Shenqinmycin 0.5%, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane 30%, dodecylbenzene sodium sulfonate 4%, lignin sodium naphthalene sulfonate 12%,
White carbon 20%, Kaolin complements to 100%.
Preparation method：By each component mix homogeneously, mechanical activation comminution is first passed through, then after comminution by gas stream, mix homogeneously, i.e.,
30.5% shenqinmycin bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane wettable powder can be obtained.
Comparative example 9:30.5% polyoxin bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane wettable powder (1:60)
Component:Polyoxin 0.5%, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane 30%, dodecylbenzene sodium sulfonate 4%, lignin sodium naphthalene sulfonate 12%,
White carbon 20%, Kaolin complements to 100%.
Preparation method：Ibid.
Embodiment 11:31% shenqinmycin bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane wettable powder (1:30)
Component:Shenqinmycin 1%, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane 30%, dodecylbenzene sodium sulfonate 3%, naphthalenesulfonic acid-formaldehyde condensate
8%, white carbon 12%, Pulvis Talci complements to 100%.
Preparation method：By each component mix homogeneously, mechanical activation comminution is first passed through, then after comminution by gas stream, mix homogeneously, i.e.,
31% shenqinmycin bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane wettable powder can be obtained.
Comparative example 10:31% shenqinmycin Tebuconazole wettable powder (1:30)
Component:Shenqinmycin 1%, Tebuconazole 30%, dodecylbenzene sodium sulfonate 3%, naphthalenesulfonic acid-formaldehyde condensate
8%, white carbon 12%, Pulvis Talci complements to 100%.
Preparation method：Ibid.
Embodiment 12:16% shenqinmycin bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane dry suspending agent (1:15)
Component:Shenqinmycin 1%, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane 15%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 8%, organosilicon
Defoamer 0.4%, sodium benzoate 0.1%, white carbon 10%, Pulvis Talci complements to 100%.Soft water 150-200%.
Preparation method：The water of 1.5-2.0 times of preparation amount of preparation is added, each component is mixed, the mixing of Jing high speed shear is equal
It is even, then by sand mill sand milling 2-3 hours, make mean diameter reach 1-5 microns, it is mould that dehydrate can be prepared by 16% Shen piperazine
Plain bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane dry suspending agent.
Comparative example 11:16% polyoxin bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane dry suspending agent (1:15)
Component:Polyoxin 1%, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane 15%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 8%, organosilicon
Defoamer 0.4%, sodium benzoate 0.1%, white carbon 10%, Pulvis Talci complements to 100%.Soft water 150-200%.
Preparation method：Ibid.
Embodiment 13:20% shenqinmycin flusilazole water dispersible granule (1:1)
Component:Shenqinmycin 10%, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane 10%, sodium lauryl sulphate 4%, polycarboxylic acids sodium 5%, anhydrous sodium sulfate
15%, Kaolin complements to 100%.
Preparation method：Each component is mixed, Jing mechanical activation comminution, then Jing comminution by gas stream, mix homogeneously, then Jing wet granulations are simultaneously
Drying, you can 20% shenqinmycin flusilazole water dispersible granule is obtained.
Comparative example 12:20% shenqinmycin difenoconazole water dispersible granule (1:1)
Component:Shenqinmycin 10%, Difenoconazole 10%, sodium lauryl sulphate 4%, polycarboxylic acids sodium 5%, anhydrous sulfur
Sour sodium 15%, Kaolin complements to 100%.
Preparation method：Ibid.
Embodiment 14:21% shenqinmycin bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane wettable powder (19:2)
Component:Shenqinmycin 19%, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane 2%, dodecylbenzene sodium sulfonate 3%, lignin sodium naphthalene sulfonate 8%, in vain
White carbon black 8%, Pulvis Talci complements to 100%.
Preparation method：By each component mix homogeneously, mechanical activation comminution is first passed through, then after comminution by gas stream, mix homogeneously, i.e.,
21% shenqinmycin bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane wettable powder can be obtained.
Comparative example 13:21% shenqinmycin prothioconazoles wettable powder (19:2)
Component:Shenqinmycin 19%, prothioconazoles 2%, dodecylbenzene sodium sulfonate 3%, lignin sodium naphthalene sulfonate 8%,
White carbon 8%, Pulvis Talci complements to 100%.
Preparation method：Ibid.
Embodiment 15:30.5% shenqinmycin bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane suspending agent (60:1)
Component：Shenqinmycin 30%, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane 0.5%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 4%, octyl phenol polyoxy
Vinyl Ether sodium sulfonate 4%, ethylene glycol 6%, XG 0.25%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water is supplied
To 100%.
Preparation method：Each component is mixed, Jing high speed shear mix homogeneously, then by sand mill sand milling 2-3 hours, make to put down
Particle diameter reaches 1-5 microns, you can 30.5% shenqinmycin bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane suspending agent is obtained.
Comparative example 14:30.5% shenqinmycin tebuconazole suspension concentrates (60:1)
Component：Shenqinmycin 30%, Tebuconazole 0.5%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 4%, octyl phenol polyoxy
Vinyl Ether sodium sulfonate 4%, ethylene glycol 6%, XG 0.25%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water is supplied
To 100%.
Preparation method：Ibid.
Three：Shenqinmycin is compounded with tetraconazole
Embodiment 16：30.5% shenqinmycin tetraconazole wettable powder (1:60)
Component:Shenqinmycin 0.5%, tetraconazole 30%, dodecylbenzene sodium sulfonate 6%, lignin sodium naphthalene sulfonate
12%, white carbon 25%, Pulvis Talci complements to 100%.
Preparation method：By tetraconazole and white carbon mixing and absorption, then other each components are added into mix homogeneously, Jing Guoji
Tool is crushed, then after comminution by gas stream, mix homogeneously, you can 30.5% shenqinmycin tetraconazole wettable powder is obtained.
Comparative example 15:30.5% polyoxin tetraconazole wettable powder (1:60)
Component:Polyoxin 0.5%, tetraconazole 30%, dodecylbenzene sodium sulfonate 6%, lignin sodium naphthalene sulfonate
12%, white carbon 25%, Pulvis Talci complements to 100%.
Preparation method：Ibid.
Embodiment 17:31% shenqinmycin tetraconazole wettable powder (1:30)
Component:Shenqinmycin 1%, tetraconazole 30%, sodium lauryl sulphate 6%, lignin sodium naphthalene sulfonate 12%, in vain
White carbon black 25%, anhydrous sodium sulfate 8%, Pulvis Talci complements to 100%.
Preparation method：By tetraconazole and white carbon mixing and absorption, then other each components are added into mix homogeneously, Jing Guoji
Tool is crushed, then after comminution by gas stream, mix homogeneously, you can 31% shenqinmycin tetraconazole wettable powder is obtained.
Comparative example 16：31% shenqinmycin prothioconazoles wettable powder (1:30)
Component:Shenqinmycin 1%, prothioconazoles 30%, sodium lauryl sulphate 6%, lignin sodium naphthalene sulfonate 12%, in vain
White carbon black 25%, anhydrous sodium sulfate 8%, Pulvis Talci complements to 100%.
Preparation method：Ibid.
Embodiment 18:20% shenqinmycin tetraconazole wettable powder (1:1)
Component:Shenqinmycin 10%, tetraconazole 10%, dodecylbenzene sodium sulfonate 3%, dodecyl naphthalene sulfonate first
Aldehyde condensate 8%, white carbon 12%, precipitated calcium carbonate complements to 100%.
Preparation method：Preparation method：By tetraconazole and white carbon mixing and absorption, then add mixing equal other each components
It is even, through mechanical activation comminution, then after comminution by gas stream, mix homogeneously, you can 20% shenqinmycin tetraconazole is obtained wettable
Property powder.
Comparative example 17:20% polyoxin tetraconazole wettable powder (1:1)
Component:Polyoxin 10%, tetraconazole 10%, dodecylbenzene sodium sulfonate 3%, dodecyl naphthalene sulfonate first
Aldehyde condensate 8%, white carbon 12%, precipitated calcium carbonate complements to 100%.
Preparation method：Ibid.
Embodiment 19:16% shenqinmycin tetraconazole suspending agent (15:1)
Component：Shenqinmycin 15%, tetraconazole 1%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 3%, octyl phenol polyoxy
Vinyl Ether sodium sulfonate 3%, methyl oleate 10%, 1,2-PD 6%, XG 0.25%, organic silicon defoamer 0.2%, benzoic acid
Sodium 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, Jing high speed shear mix homogeneously, then by sand mill sand milling 2-3 hours, make to put down
Particle diameter reaches 1-5 microns, you can 16% shenqinmycin tetraconazole suspending agent is obtained.
Comparative example 18:16% shenqinmycin prothioconazoles suspending agent (15:1)
Component：Shenqinmycin 15%, tetraconazole 1%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 3%, octyl phenol polyoxy
Vinyl Ether sodium sulfonate 3%, methyl oleate 10%, 1,2-PD 6%, XG 0.25%, organic silicon defoamer 0.2%, benzoic acid
Sodium 0.4%, soft water complements to 100%.
Preparation method：Ibid.
Embodiment 20:31% shenqinmycin tetraconazole dry suspending agent (30:1)
Component:Shenqinmycin 30%, tetraconazole 1%, sodium lignin sulfonate 10%, lignin sodium naphthalene sulfonate 10% have
Machine silicon defoaming agent 0.4%, sodium benzoate 0.1%, white carbon 10%, Pulvis Talci complements to 100%.Soft water 150-200%.
Preparation method：The water of 1.5-2.0 times of preparation amount of preparation is added, each component is mixed, the mixing of Jing high speed shear is equal
It is even, then by sand mill sand milling 2-3 hours, make mean diameter reach 1-5 microns, it is mould that dehydrate can be prepared by 31% Shen piperazine
Plain tetraconazole dry suspending agent.
Comparative example 19:31% polyoxin tetraconazole dry suspending agent (30:1)
Component:Polyoxin 30%, tetraconazole 1%, sodium lignin sulfonate 10%, lignin sodium naphthalene sulfonate 10% have
Machine silicon defoaming agent 0.4%, sodium benzoate 0.1%, white carbon 10%, Pulvis Talci complements to 100%.Soft water 150-200%.
Preparation method：Ibid.
Embodiment 21:30.5% shenqinmycin tetraconazole suspending agent (60:1)
Component：Shenqinmycin 30%, tetraconazole 0.5%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 4%, octylphenol polyethylene
Oxygen vinyl Ether sodium sulfonate 4%, ethylene glycol 6%, XG 0.25%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water are mended
Enough to 100%.
Preparation method：Each component is mixed, Jing high speed shear mix homogeneously, then by sand mill sand milling 2-3 hours, make to put down
Particle diameter reaches 1-5 microns, you can 30.5% shenqinmycin tetraconazole suspending agent is obtained.
Comparative example 20:30.5% shenqinmycin prothioconazoles suspending agent (60:1)
Component：Shenqinmycin 30%, prothioconazoles 0.5%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 4%, octylphenol polyethylene
Oxygen vinyl Ether sodium sulfonate 4%, ethylene glycol 6%, XG 0.25%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water are mended
Enough to 100%.
Preparation method：Ibid.
Find through experiment, the epoxiconazole, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane, tetraconazole in above example mutually can be replaced, to be formed
New embodiment.No longer carry out repetition to enumerate to this, it is noted that for those skilled in the art,
On the premise of without departing from principle of the present invention, some improvements and modifications can also be made, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Sigatoka, wheat leaf are prevented and treated with pesticidal preparations obtained in above-described embodiment and embroiders disease.
1st, test process：Test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination
Test dosing and three concentration for the treatment of are set respectively according to the difference of each composition, comparison medicament is 12.5% fluorine of commercially available pesticide respectively
Ring azoles SC, 1% shenqinmycin SC and blank clean water experiment.
2nd, test method：Each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after investigating before dispenser and preventing and treating
Method is：Per the sampling of cell diagonal, 10 plants are often clicked.It is classified with the fungus ball number on every plant of grain, result of the test see the table below 4,
5：
Sigatoka investigates grade scale：
0 grade：It is disease-free；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%-15% of whole leaf area；
5 grades：Lesion area accounts for the 16%-25% of whole leaf area；
7 grades：Lesion area accounts for the 26%-50% of whole leaf area；
9 grades：Lesion area accounts for more than the 50% of whole leaf area；
Wheat leaf embroiders disease investigation grade scale：
Wheat leaf is embroidered sick field investigation standard and adopts following stage division：
0 grade：It is disease-free；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%-25% of whole leaf area；
5 grades：Lesion area accounts for the 26%-50% of whole leaf area；
7 grades：Lesion area accounts for the 51%-75% of whole leaf area；
9 grades：Lesion area accounts for more than the 76% of whole leaf area；
4 shenqinmycin of table compounds the prevention effect to sigatoka with epoxiconazole
4 field efficacy result of table shows, compares 1 in weight：60-60：In the range of 1, shenqinmycin and epoxiconazole compound synergic
Effect is obvious, and particularly 1：30-30：In the range of 1, especially 1：15-15：In the range of 1, there is excellent preventing and treating to Fructus Musae tikka
Effect, prevention effect are better than single dose kind.
5 embodiment of table is compared to the prevention effect that wheat leaf embroiders disease with comparative example
Inventor has found that other antibioticses antibacterial are compounded with epoxiconazole, and shenqinmycin is multiple with other triazole bactericidal agents
Match somebody with somebody, compare 1 in weight：60-60：Also there is in the range of 1 certain Complex synergism.Because length is limited, do not enumerate, only with
Most representational jingganmycin, polyoxin, propiconazole, hexaconazole construct comparative example and embodiment and carry out preventive effect contrast,
As 5 field efficacy result of table shows, compare 1 in weight：60-60：(particularly 1 in the range of 1：30-30：1, especially 1：15-15：
1) with epoxiconazole Complex synergism substantially, the prevention effect to plant disease is better than other antibioticses and sterilizes shenqinmycin
Agent and the prevention effect of epoxiconazole compounding, are also better than the compounding effect of shenqinmycin and other triazole bactericidal agents.
The field test of scab of cucumber, wheat powdery mildew is prevented and treated with pesticidal preparations obtained in above-described embodiment.
1st, test process：Test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination
Test dosing and three concentration for the treatment of are set respectively according to the difference of each composition, comparison medicament is commercially available pesticide 400g/l fluorine respectively
Silicon azoles cream, 1% shenqinmycin SC and blank clean water experiment.
2nd, test method：Each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after investigating before dispenser and preventing and treating
Method is：Per the sampling of cell diagonal, 10 plants are often clicked.It is classified with the fungus ball number on every plant of grain, result of the test see the table below 6,
7：
Scab of cucumber investigates grade scale：
0 grade：It is asymptomatic
1 grade：There is visible water soaking mode chlorisis point or yellow point in blade；
3 grades：There is minority withered spot in blade, and withered spot accounts for blade area below 10%；
5 grades：Blade withered spot accounts for blade area 11%-30%, and withered spot occurs in petiole；
7 grades：Blade withered spot accounts for blade area 31%-60% or depression speckle occurs in petiole, sometimes petiole cracking；
9 grades：Blade withered spot accounts for blade area more than 61% or petiole fractures.
Wheat powdery mildew investigates grade scale：
0 grade：It is disease-free；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%-15% of whole leaf area；
5 grades：Lesion area accounts for the 16%-25% of whole leaf area；
7 grades：Lesion area accounts for the 26%-50% of whole leaf area；
9 grades：Lesion area accounts for less than the 50% of whole leaf area；
6 shenqinmycin of table compounds the prevention effect to scab of cucumber with bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane
6 field efficacy result of table shows, compares 1 in weight：60-60：In the range of 1, shenqinmycin and bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane compound synergic
Effect is obvious, and particularly 1：30-30：In the range of 1, especially 1：15-15：In the range of 1, scab of cucumber is had excellent anti-
Effect is controlled, prevention effect is better than single dose kind.
7 embodiment of table is compared to the prevention effect of wheat powdery mildew with comparative example
Inventor has found that other antibioticses antibacterial are compounded with bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane, and shenqinmycin is multiple with other triazole bactericidal agents
Match somebody with somebody, compare 1 in weight：60-60：Also there is in the range of 1 certain Complex synergism.Because length is limited, do not enumerate, only with
Most representational polyoxin, propiconazole, Tebuconazole, Difenoconazole, prothioconazoles construct comparative example and embodiment is entered
Row preventive effect is contrasted, and such as 7 field efficacy result of table shows, compares 1 in weight：60-60：In the range of 1, (particularly weight compares 1：30-30：
1, weight compares 1：15-15：In the range of 1), shenqinmycin with bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane Complex synergism substantially, imitate by the preventing and treating to plant disease
Really, it is better than the prevention effect of other antibioticses antibacterial and bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane compounding, is also better than shenqinmycin and kills with other triazole type
The prevention effect of microbial inoculum compounding.
With the test of pesticidal preparations preventing and treating powdery mildew of strawberry, hami melon powdery mildew obtained in above-described embodiment.
1st, test process：Test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination
Test dosing and three concentration for the treatment of are set respectively according to the difference of each composition, comparison medicament is commercially available pesticide 12.5% 4 respectively
Fluorine ether azoles aqueous emulsion, 1% shenqinmycin SC and blank clean water experiment.
2nd, test method：Each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after investigating before dispenser and preventing and treating
Method is：Per the sampling of cell diagonal, 10 plants are often clicked.It is classified with the fungus ball number on every plant of grain, result of the test see the table below 8,
9：
Powdery mildew of strawberry, hami melon powdery mildew test survey grade scale：
0 grade：It is disease-free；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%-15% of whole leaf area；
5 grades：Lesion area accounts for the 16%-25% of whole leaf area；
7 grades：Lesion area accounts for the 26%-50% of whole leaf area；
9 grades：Lesion area accounts for less than the 50% of whole leaf area；
8 shenqinmycin of table compounds the prevention effect to powdery mildew of strawberry with tetraconazole
8 field efficacy result of table shows, compares 1 in weight：60-60：In the range of 1, shenqinmycin is increased with tetraconazole compounding
Effect effect is obvious, and particularly 1：30-30：In the range of 1, especially 1：15-15：In the range of 1, powdery mildew of strawberry is had excellent
Prevention effect, prevention effect are better than single dose kind.
9 embodiment of table is compared to the prevention effect of hami melon powdery mildew with comparative example
Inventor has found other antibioticses antibacterial with tetraconazole compounding, shenqinmycin and other triazole bactericidal agents
Compounding, compares 1 in weight：60-60：Also there is in the range of 1 certain Complex synergism.Because length is limited, do not enumerate, only
Comparative example and embodiment are constructed with most representational polyoxin, prothioconazoles carries out preventive effect contrast, such as 9 field efficacy of table
As a result show, compare 1 in weight：60-60：In the range of 1, shenqinmycin is obvious with tetraconazole Complex synergism, to phytopathy
Harmful prevention effect, is better than the prevention effect of other antibioticses antibacterial and tetraconazole compounding, be also better than shenqinmycin with
The compounding effect of prothioconazoles.
The above is the preferred embodiment of the present invention, it is noted that for those skilled in the art
For, on the premise of without departing from principle of the present invention, some improvements and modifications can also be made, these improvements and modifications
Should be regarded as protection scope of the present invention.
Claims (9)
1. a kind of bactericidal composition, it is characterised in that effective ingredient contains shenqinmycin and triazole bactericidal agent, the triazole type
Antibacterial is 1 for the weight ratio of epoxiconazole, bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane or tetraconazole, the shenqinmycin and triazole bactericidal agent：60～60：
1。
2. bactericidal composition according to claim 1, it is characterised in that the weight of the shenqinmycin and triazole bactericidal agent
Amount is than being 1：30～30：1.
3. bactericidal composition according to claim 2, it is characterised in that the weight of the shenqinmycin and triazole bactericidal agent
Amount is than being 1：15～15：1.
4. according to arbitrary described bactericidal composition in claims 1 to 3, it is characterised in that also contain in the bactericidal composition
There is pesticidal preparations auxiliary element, be adapted to the dosage form for agriculturally using to make.
5. bactericidal composition according to claim 4, it is characterised in that the pesticidal preparations auxiliary element is solvent, breast
One or more in agent, dispersant, stabilizer, antifreezing agent or wetting agent；The dosage form of the bactericidal composition be suspending agent,
Dry suspending agent, water dispersible granules or wettable powder.
6. application of the arbitrary described bactericidal composition of claim 1 to 5 in the pesticidal preparations for preparing controlling plant diseases.
7. the application of bactericidal composition according to claim 6, it is characterised in that prevent and treat rice sheath blight disease, rice in preparation
Answering in the pesticidal preparations of pestilence, false smut, wheat rust, powdery mildew, banana freckle, Fructus Musae black disease disease or graw mold of tomato
With.
8. the application process of the arbitrary described bactericidal composition of claim 1 to 5, it is characterised in that active component shenqinmycin
Compare 1 with weight with triazole bactericidal agent：60～60：1 apply, its be mixed in proportion after apply, be administered simultaneously in proportion or by than
Example is applied successively.
9. the application process of bactericidal composition according to claim 8, it is characterised in that active component shenqinmycin and three
Azoles fungicide compares 1 with weight：30～30：1 apply, its be mixed in proportion after apply, be administered simultaneously in proportion or in proportion according to
Secondary administration.
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| CN105248432A (en) * | 2015-11-16 | 2016-01-20 | 周红梅 | Anti-fungal composition containing phenazine-1-carboxylic acid and dimethomorph |
| CN106719695A (en) * | 2017-04-09 | 2017-05-31 | 佛山市瑞生通科技有限公司 | A kind of bactericidal composition of the mycin of second containing fen and tetraconazole |
| CN108651475A (en) * | 2018-06-24 | 2018-10-16 | 合肥红佳科技信息有限公司 | A kind of bactericidal composition containing shenqinmycin and triticonazole |
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| CN104957152A (en) | 2015-10-07 |
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Address after: 621000 South Section of Mianzhou Avenue, Mianyang City, Sichuan Province, 329 Patentee after: Sichuan LEL Crop Science Co., Ltd. Address before: 621000 No.77 Shuangyong Road, Fenggu Town, Fucheng District, Mianyang City, Sichuan Province Patentee before: Sichuan LEL Crop Science Co., Ltd. |