CN104904732B

CN104904732B - Sterilization composition containing ZJ5337 and triazole bactericide

Info

Publication number: CN104904732B
Application number: CN201410088235.0A
Authority: CN
Inventors: 胡伟群; 许天明; 张蕊蕊; 朱卫刚; 陈杰; 孔小林
Original assignee: Zhejiang Chemical Industry Research Institute Co Ltd; Sinochem Lantian Co Ltd
Current assignee: Zhejiang Chemical Industry Research Institute Co Ltd; Sinochem Corp; Sinochem Lantian Co Ltd
Priority date: 2014-03-11
Filing date: 2014-03-11
Publication date: 2017-05-10
Anticipated expiration: 2034-03-11
Also published as: CN104904732A

Abstract

The invention provides a sterilization composition. The sterilization composition contains ZJ5337 and a triazole bactericide according to a mass ratio of 20:1-1:20. The sterilization composition has the advantages of good sterilization effect, low medication cost, and difficult generation of drug resistance, and is especially suitable for controlling rice sheath blight, rice blast, rice false smut or rice brown spot.

Description

A kind of bactericidal composition containing ZJ5337 and triazole bactericidal agent

Technical field

The invention belongs to composition pesticide field, and in particular to a kind of bactericidal composition for preventing and treating rice disease.

Background technology

Rice disease such as rice sheath blight disease, false smut, rice blast, Hemintho-sporum leaf spot of rice plants are the Major Diseases in Oryza sativa L. producing region, There is large area throughout the year, it is very harmful, have a strong impact on the yield and quality of Oryza sativa L., it has also become of rice high yield stable yields Outstanding problem.In the real process of agricultural production, controlling disease is easiest to generate the problem that the drug-fast generation of pathogenic bacteria.Penta Azoles alcohol, Difenoconazole, hexaconazole, propiconazole, prothioconazoles, epoxiconazole, RH-7592 are used as triazole bactericidal agent kind, cause of disease Bacterium produces the speed of resistance to it quickly, and it is increasing to be administered alone dose, and dosage conference suppresses plant growth.If even Continuous medication is more than three times, it is more likely that causes crop leaf to diminish, be hardened, becoming fragile, the poisoning situation such as blackening.Therefore by triazole type Antibacterial and the bactericide compounded of other mechanisms of action will be science, the development need of rational use of drug.

ZJ5337（Chemical name：Fluorine isopropyl -4- quinoline the esters of 2,3,8- trimethyl -6- of carbonic acid benzyl ester seven）It is Chinese patent The quinolines that seven fluorine isopropyls of CN201310571013.x reports replace, it is prepared and its bactericidal activity is not disclosed Report, finds ZJ5337 wide sterilization spectrums in the research of early stage, there is preferable activity to various fungal diseases, especially rice blast and The powdery mildew of vegetable melon and fruit has specially good effect.

Tebuconazole（Tebuconazole）：Chemical name：(RS) the p- chlorphenyl -4,4- dimethyl -3- (1H-1,2,4 of -1- Triazol-1-yl methyl) amyl- 3- alcohol.Category triazole bactericidal agent, is sterol demethylation inhibitors, is for Important Economic crop Seed treatment or face sprinkling efficient germicide, can effectively prevent and treat various rust, powdery mildew, the filigree of cereal crop Disease, root rot, head blight, smut and kind pass zonate spot etc..Can be used as seed treatment and foliar spray agent, not only activity Height, and the lasting period is long, to many crops and dicotyledon safety.Tebuconazole wide sterilization spectrum, is mainly used in preventing and treating Semen Tritici aestivi, flower Various fungal diseases on the crops such as life, vegetable, Fructus Musae, Fructus Mali pumilae, pears.

Difenoconazole also Cheng Difenoconazoles（Difenoconazole）, chemical name：Chloro- 4- [4- methyl-the 2- of cis- trans- 3- （1H-1,2,4- triazol-1-yl methyl）Ring -2- the bases of -1,3- dichloros penta] phenyl -4- chlorphenyl ethers.Difenoconazole is a kind of three Azoles fungicide, is sterol demethylation inhibitors, suppresses the biosynthesiss of cell wall sterol, prevents the growth of funguses.Sterilization Spectrum is wide, and foliar treatment or seed treatment can improve the yield and guaranteed quality of crop.To Ascomycetes, Basidiomycetess and including chain Lattice spore category, Ascochyta, Cercospora, Colletotrichum, ball Cuo Pseudomonas, Phoma, Ramularia, Septoria, black star bacterium Belong to and partly know disease interior, Erysiphaceae, Uredinales and some Seedborne pathogens have lasting protection and therapeutical effect.

Hexaconazole（Hexaconazole）, chemical name：(RS) -2- (2,4- Dichlorobenzene base）-1-（1H-1,2,4- triazoles- 1- bases）- hex- 2- alcohol.Category azoles fungicide, sterol demethylation inhibitors have protection to fungus-caused disease and treat and make With.The biosynthesiss of the cell membrane important composition composition ergosterol of destruction and prevention pathogenic bacteria, cause cell membrane to be formed, and make Pathogenic bacteria is dead.The disease caused by ascomycetess, basidiomycetes and Fungi Imperfecti can be effectively prevented and treated, especially to Basidiomycetess and ascomycetess The disease that guiding principle causes such as powdery mildew, rust, scab, brown spot, anthrax etc. have excellent protection and eradicant action, to Oryza sativa L. Banded sclerotial blight also has good preventive effect.

Propiconazole（Propiconazole）, chemical name：1-[2-（2,4- Dichlorobenzene base）- 4- n-pro-pyl -1,3- dioxies Penta ring -2- ylmethyls] -1- hydrogen -1,2,4- triazoles.Propiconazole is a kind of systemic fungicide with protection and therapeutical effect, Can be absorbed by root, stem, leaf portion, and soon can be conducted upwards in plant body, propiconazole can prevent and treat ascomycetess, basidiomycetes and Disease caused by Fungi Imperfecti.Particularly to take-all, powdery mildew, rust, root rot, water rice bakanae disease, Fructus Musae tikka Disease has preferable prevention effect.But it is invalid to oomycetes disease.

Prothioconazoles（Prothioconazole）, chemical name：（RS）-2-[2-（1- chlorine cyclopropyl）-3-（2- chlorobenzenes Base）- 2- hydroxypropyls] -2,4- dihydro -1,2,4- triazole -3- thioketone.Prothioconazoles are that a kind of New-type wide-spectrum triazole thioketone is killed Microbial inoculum, is mainly used in preventing and treating numerous diseases such as frumentum, wheat and barley, legume crop, in its mechanism of action suppression fungus before sterol Demethylation effect on body --- lanosterol or 2,4- methylene dihydro Pilus Caprae seu Oviss steroid 14.

Epoxiconazole（Epoxiconazole）, chemical name：(2RS, 3RS) -1- [3- (2 monochloro-benzene base) -2,3 one epoxies - 2-（4 phenyl-monofluoride bases）Propyl group] triazole of one 1 hydrogen one 1,2,4 one.Epoxiconazole is triazole bactericidal agent, to a series of cereal crops Such as damping-off, powdery mildew, the multiple diseases of eyeprint disease ten have good preventive and therapeutic effect, and can prevent and treat sugar beet, Semen arachidis hypogaeae, oil The diseases such as dish, lawn, coffee, Oryza sativa L. and fruit tree.Not only have and protect, treat and root out activity well, and with interior suction Preferably residual activity.

RH-7592（Fenbuconazole）It is called azoles bacterium nitrile, cyanophenyl azoles, chemical name：4-（4- chlorphenyls）- 2- phenyl one 2 (1H-1,2,4- triazol-1-yl methyl) butyronitrile.RH-7592 is triazole type systemic fungicide, can prevent the pathogenic bacteria spore for having germinateed Crop plant tissue is invaded, suppresses the elongation of mycelia.Use incubation period in pathogenic bacteria, the development of pathogenic bacteria can be prevented；Use after morbidity, energy Deform spore of future generation, lose infection ability, there is preventive effect and therapeutical effect to disease.Fruit tree and land for growing field crops are made Many diseases of thing, Oryza sativa L., vegetable and garden crop are effective.

ZJ5337 and Tebuconazole, Difenoconazole, hexaconazole, propiconazole, prothioconazoles, fluorine ring are had no in prior art The compounding report of azoles, RH-7592.

The content of the invention

It is an object of the invention to provide a kind of component is rationally, bactericidal effect is good, and drug cost is low, is not likely to produce Drug resistance Bactericidal composition.

It is to reach goal of the invention the technical solution used in the present invention：

A kind of bactericidal composition, containing ZJ5337 and triazole bactericidal agent, the triazole bactericidal agent is selected from Tebuconazole, benzene One kind, two or three combination of the above in ether methyl cyclic-azole, hexaconazole, propiconazole, prothioconazoles, epoxiconazole and RH-7592, institute It is 20 that ZJ5337 is stated with the mass ratio of triazole bactericidal agent:1～1:20.

Used as preferred mode, above-mentioned ZJ5337 is 10 with the mass ratio of triazole bactericidal agent:1～1:10；Above-mentioned triazole One kind in propiconazole, prothioconazoles, the RH-7592 of series bactericidal agent, two or three.

Bactericidal composition of the present invention can be formulated into antibacterial, when antibacterial is formulated into, the sterilization Agent contains acceptable carrier on bactericidal composition and pesticide, and the bactericidal composition accounts for the 1%～80% of antibacterial gross weight, enters One step ground is preferably bactericidal composition and accounts for the 5%～80% of antibacterial gross weight.

Antibacterial of the present invention can be configured to any dosage form for agriculturally allowing, preferably water dispersible granules, suspension Agent, wettable powder, aqueous emulsion or microemulsion.Used as preferred mode, each composition quality proportioning is in the water dispersible granules： Bactericidal composition 5～80%, dispersant 3～12%, wetting agent 1-8%, disintegrating agent 1～10%, binding agent 0～8%, remaining be filler； Each composition quality proportioning is in the suspending agent：Bactericidal composition 1～80%, dispersant 2～6%, wetting agent 0.5-5%, thickening agent 0～2%, antifreezing agent 0～5%, preservative 0～0.2%, defoamer 0～0.5%；Each composition quality proportioning in the wettable powder For：Bactericidal composition 5～80%, dispersant 5～10%, wetting agent 0-10%, remaining be filler；It is each into sub-prime in the aqueous emulsion Measuring proportioning is：It is bactericidal composition 1～80%, emulsifying agent 3～10%, solvent 5～30%, stabilizer 0～1%, thickening agent 0～1%, anti- Rotten agent 0～2%；Each composition quality proportioning is in the microemulsion：Bactericidal composition 1～80%, emulsifying agent 15～25%, help emulsifying Agent 5～10%, solvent 10～30%, stabilizer 0～5%.

For water dispersible granules, those skilled in the art are very familiar with completing the present invention using corresponding auxiliary agent.Dispersion Agent is selected from polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate；Wetting agent is selected from alkyl sulfate, alkylsulfonate, naphthalene Sulfonate；Disintegrating agent is selected from ammonium sulfate, carbamide, sucrose, glucose；Binding agent is selected from kieselguhr, corn starch, PVA, carboxymethyl （Ethyl）Cellulose family；Filler is selected from kieselguhr, Kaolin, white carbon, fine particle calcium carbonate, Pulvis Talci, attapulgite, potter's clay.

To wettable powder, the auxiliary agent that can be used has：Dispersant is selected from polycarboxylate, lignosulfonates, alkylnaphthalene sulphur Hydrochlorate；Wetting agent is selected from alkyl sulfate, alkylsulfonate, naphthalene sulfonate；Filler is selected from ammonium sulfate, carbamide, sucrose, Fructus Vitis viniferae Sugar, kieselguhr, Kaolin, white carbon, fine particle calcium carbonate, Pulvis Talci, attapulgite, potter's clay.

To suspending agent, the auxiliary agent that can be used has：Dispersant selected from polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate, TERSPERSE2425（Huntsman Corporation of the U.S. produces, alkyl naphthalene sulfonic acid salt）；Wetting agent is selected from the poly- base of alkylphenol-polyethenoxy Ether formaldehyde condensation products sulfate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyoxyethylene groups ether phosphate, alkyl sulfide Hydrochlorate, alkylsulfonate, naphthalene sulfonate, TERSPERSE2500（Huntsman Corporation of the U.S. produces）；Thickening agent selected from xanthan gum, Polyvinyl alcohol, bentonite；Preservative is selected from formaldehyde, benzoic acid, sodium benzoate；Defoamer is silicone defoamer；Antifreezing agent Selected from ethylene glycol, Propylene Glycol, glycerol, carbamide, inorganic salts such as Sodium Chloride.

To aqueous emulsion, the auxiliary agent that can be used has：Emulsifying agent is selected from Nonyl pheno（EO=10）Ether phosphate, triphen Ethyl -phenol polyoxyethylene ether phosphate（Agriculture breast 600# phosphate ester）, Nongru-700 #, agriculture breast 2201#, Span-60#, emulsifying agent T- 60th, TX-10, agriculture breast 1601#, agriculture breast 600#, agriculture breast 400#；Solvent is selected from dimethylbenzene, toluene, Ketohexamethylene, solvent naphtha（The trade mark： S-150、S-180、S-200）；Stabilizer is selected from triphenyl phosphite, epoxychloropropane, acetic anhydride；Thickening agent is selected from xanthan gum, gathers Vinyl alcohol, bentonite, aluminium-magnesium silicate；Preservative is selected from methanol, benzoic acid, sodium benzoate.

To microemulsion, the auxiliary agent that can be used has：Emulsifying agent is selected from calcium dodecyl benzene sulfonate（Agriculture breast 500#）, agriculture breast 700#, agriculture breast 2201#, this disk-60#, tween 80-60#, TX-10, agriculture breast 1601, agriculture breast 600#, agriculture breast 400#；Co-emulsifier Selected from methanol, isopropanol, n-butyl alcohol, ethanol；Solvent is selected from Ketohexamethylene, N-Methyl pyrrolidone, dimethylbenzene, toluene, solvent naphtha （The trade mark：S-150、S-180、S-200）；Stabilizer is selected from triphenyl phosphite, epoxychloropropane.

The compositionss of the present invention can be provided in finished product preparation form, i.e., each material is blended in compositionss；Compositionss Composition can also be provided with unit dose preparation, using front in bucket（Tank）In directly mix.The concentrate of the present invention is generally mixed with water Conjunction obtains the concentration of required active substance.

The bactericidal composition that the present invention is provided is particularly suitable for being used to prevent and treat rice sheath blight disease, rice blast, false smut or water Helminthosporium of brown spot of rice.

The present invention provide bactericidal composition compared with prior art, with advantages below：

（1）Two kinds of active components has Synergistic mode, and bactericidal effect is good, to rice sheath blight disease, rice blast, false smut Or Hemintho-sporum leaf spot of rice plants has specially good effect；

（2）It is capable of achieving to prevent and treat multiple diseases simultaneously, slows down resistance generation；

（3）Drug cost is low；

（4）Treatment plus protective effect, it is good to crop safety, meet the security requirement of pesticidal preparations.

Specific embodiment

The present invention is further described with reference to specific embodiment, but does not limit the invention to these tools Body embodiment.One skilled in the art would recognize that present invention encompasses being potentially included in Claims scope All alternatives, improvement project and equivalents.

First, bioassay

The Toxicity Determination of embodiment 1, ZJ5337 and triazole bactericidal agent compounding to rice sheath blight disease

Rhizoctonia solani Kuhn Rhizoctonia solani test methods are referred to《People's Republic of China's agricultural industry criteria NY/T1156.5-2006》.Select two leaves wholeheartedly, the consistent potted plant rice seedling of growing way, dry naturally after spraying treatment, 24h or so Be inoculated with, being jetted through every basin rice seedling base portion central authorities clamping connection one piece of sheath blight fungus mycelia block of medicine, truffle should not with basin soil and Water layer is contacted.Postvaccinal examination material is moved in humidistat to be cultivated, 26 DEG C of temperature, humidity 80-90%, daily observation rice growth shape Condition, investigation prevention effect when blank is fully fallen ill after 5 days.By the line between prevention effect and the logarithm value of series concentration Property regression analyses, obtain the EC of each medicament₅₀Value, further according to the abundant methods of Sun Yun co-toxicity coefficient is calculated（CTC）.

1）Investigation stage division：

0 grade：Do not fall ill；

1 grade：First sheath is fallen ill；

3 grades：First blade and the second sheath are fallen ill；

5 grades：Second blade and the 3rd sheath are fallen ill；

7 grades：Whole strain morbidity.

2）Computational methods：

According to survey data, the disease index and prevention effect of each process are calculated.

The calculating of co-toxicity coefficient.Each is calculated with disease index and processes prevention effect, use " DPS data handling systems " 3.11 Professional version carries out data analysis statistical, obtains regression straight line EC₅₀, correlation coefficient.It is each by what is determined according to Sun Yunpei (Y-P Sun) methods The EC of process₅₀It is converted into actual poison exponent (ATI)；According to the proportioning of mixture, theoretical toxicity index (TTI) is obtained, by following Formula calculates the co-toxicity coefficient (CTC) of mixture.

Toxicity index × the B of content (%)+Bs of the toxicity index × A of mixed toxicity index (the TTI)=A of theory in mixed Content (%) in mixed

If co-toxicity coefficient is more than 120, show there is potentiation；If being significantly lower than 100（Less than 80）, it is shown to be antagonism work With；Between 100～120, summation action is shown to be.

Table 1：ZJ5337 compounds the Toxicity Determination result to rice sheath blight disease with triazole bactericidal agent

Medicament

EC₅₀（μg/mL）

ATI

TTI

Co-toxicity coefficient CTC

ZJ5337（A）	87.11	100.00
					Tebuconazole（B）	31.18	279.38
Difenoconazole（C）	40.36	215.83
					Hexaconazole（D）	20.87	417.39
Propiconazole (E)	12.28	709.36
					Prothioconazoles（F）	35.80	243.32
Epoxiconazole（G）	14.32	608.31
					RH-7592（H）	17.33	502.65
A+B(20：1)	192.17	192.17	108.54	177.05
					A+B(15：1)	198.97	198.97	111.21	178.91
A+B(10：1)	262.70	262.70	116.31	225.86
					A+B(5：1)	307.27	307.27	129.90	236.55
A+B(3：1)	386.47	386.47	144.85	266.82
					A+B(1：1)	568.60	568.60	189.69	299.75
A+B(1：3)	705.92	705.92	234.54	300.99
					A+B(1：5)	643.35	643.35	249.48	257.87
A+B(1：10)	557.33	557.33	263.07	211.85
					A+B(1：15)	511.21	511.21	268.17	190.63
A+B(1：20)	487.19	487.19	270.84	179.88
					A+C(20：1)	43.2	201.64	105.52	191.10
A+C(15：1)	41.55	209.65	107.24	195.50
					A+C(10：1)	34.15	255.08	110.53	230.78
A+C(5：1)	26.55	328.10	119.31	275.01
					A+C(3：1)	20.16	432.09	128.96	335.07
A+C(1：1)	14.8	588.58	157.92	372.72
					A+C(1：3)	13.88	627.59	186.87	335.84
A+C(1：5)	11.25	774.31	196.53	394.00
					A+C(1：10)	13.25	657.43	205.30	320.23
A+C(1：15)	18.03	483.14	208.59	231.62
					A+C(1：20)	19.77	440.62	210.31	209.50

A+D(20：1)	40.3	216.15	115.11	187.77
					A+D(15：1)	40.01	217.72	119.84	181.68
A+D(10：1)	32.55	267.62	128.85	207.69
					A+D(5：1)	25.47	342.01	152.90	223.68
A+D(3：1)	18.33	475.23	179.35	264.98
					A+D(1：1)	11.35	767.49	258.70	296.68
A+D(1：3)	7.69	1132.77	338.04	335.10
					A+D(1：5)	7.69	1132.77	364.49	310.78
A+D(1：10)	10.66	817.17	388.54	210.32
					A+D(1：15)	12.54	694.66	397.55	174.73
A+D(1：20)	14.37	606.19	402.28	150.69
					A+E(20：1)	35.21	247.40	129.02	191.76
A+E(15：1)	30.22	288.25	138.09	208.75
					A+E(10：1)	22.17	392.92	155.40	252.85
A+E(5：1)	17.47	498.63	201.56	247.38
					A+E(3：1)	15.47	563.09	252.34	223.15
A+E(1：1)	5.64	1544.50	404.68	381.66
					A+E(1：3)	4.69	1857.36	557.02	333.45
A+E(1：5)	3.68	2367.12	607.80	389.46
					A+E(1：10)	4.88	1785.04	653.96	272.96
A+E(1：15)	5.57	1563.91	671.28	232.98
					A+E(1：20)	6.02	1447.01	680.34	212.69
A+F(20：1)	46.22	188.47	106.82	176.43
					A+F(15：1)	40.21	216.64	108.96	198.83
A+F(10：1)	32.47	268.28	113.03	237.35
					A+F(5：1)	26.35	330.59	123.89	266.85
A+F(3：1)	20.31	428.90	135.83	315.76
					A+F(1：1)	15.46	563.45	171.66	328.24
A+F(1：3)	11.05	788.33	207.49	379.93
					A+F(1：5)	12.66	688.07	219.43	313.57

A+F(1：10)	14.05	620.00	230.29	269.22
					A+F(1：15)	18.03	483.14	234.36	206.15
A+F(1：20)	18.14	480.21	236.50	203.05
					A+G(20：1)	43.21	201.60	124.21	162.31
A+G(15：1)	38.77	224.68	131.77	170.51
					A+G(10：1)	28.38	306.94	146.21	209.93
A+G(5：1)	17.36	501.79	184.72	271.65
					A+G(3：1)	13.68	636.77	227.08	280.42
A+G(1：1)	7.32	1190.03	354.16	336.02
					A+G(1：3)	5.32	1637.41	481.23	340.25
A+G(1：5)	5.12	1701.37	523.59	324.94
					A+G(1：10)	6.78	1284.81	562.10	228.57
A+G(1：15)	8.03	1084.81	576.54	188.16
					A+G(1：20)	8.35	1043.23	584.10	178.60
A+H(20：1)	17	512.41	119.64	173.24
					A+H(15：1)	42.03	207.26	125.78	187.79
A+H(10：1)	36.88	236.20	137.49	225.71
					A+H(5：1)	28.07	310.33	168.74	283.34
A+H(3：1)	18.22	478.10	203.10	305.05
					A+H(1：1)	14.06	619.56	306.21	356.05
A+H(1：3)	7.99	1090.24	409.31	348.32
					A+H(1：5)	6.11	1425.70	443.68	369.06
A+H(1：10)	5.32	1637.41	474.92	259.80
					A+H(1：15)	7.06	1233.85	486.63	192.27
A+H(1：20)	9.31	935.66	492.77	183.00

The Toxicity Determination of embodiment 2, ZJ5337 and triazole bactericidal agent compounding to rice green smut

Ustilaginoidea virens test method is referred to《People's Republic of China (PRC) agricultural industry criteria NY/T1156.5-2006》 Dry mycelial weight method.Ustilaginoidea virens（Ustilginoide virens）By the culture of this laboratory storage, in 100ml PSB trainings 3 pieces of the Ustilaginoidea virens mycelia block (diameter 5mm) cultivated in PDA culture medium 14 days, 26 DEG C of constant temperature are inoculated with foster base The training spore hyphal suspension of 14 days is shaken, elimination mycelia is crossed with monolayer sterilizing hospital gauze, spore suspension is for test.Each list Agent and mixture press respectively the series concentration of pilot study design, are added in PSB fluid mediums and make pastille fluid medium. Diluent 20ml is filled in triangular flask per 50ml, per 4 bottles of concentration.Set blank simultaneously.The process is in strict accordance with sterile working. Add per triangular flask and cultivate standby strain 2mI, 26 DEG C of constant temperature shake 12 days (rotating speed 120r/min) of training, static gas wave refrigerator 2 days.Pour out Mycelia, 60 DEG C of drying claim dry mycelial weight.The fungistatic effect of each chemicals treatment is calculated, by the probit value and serial medicament of suppression ratio Linear regression analyses between the logarithm value of concentration, obtain the EC of each medicament₅₀, further according to the abundant methods of Sun Yun co-toxicity coefficient is calculated （CTC）.

Suppression ratio %=（Blank dry mycelial weight-chemicals treatment dry mycelial weight）× 100/ blank dry mycelial weight.

Table 2：ZJ5337 compounds the Toxicity Determination result to rice green smut with triazole bactericidal agent

Medicament	EC₅₀（μg/mL）	ATI	TTI	Co-toxicity coefficient CTC
					ZJ5337（A）	11.99
Tebuconazole（B）	0.81	1480.25
					Difenoconazole（C）	0.66	1816.67
Hexaconazole（D）	0.78	1537.18
					Propiconazole (E)	0.76	1577.63
Prothioconazoles（F）	0.61	1965.57
					Epoxiconazole（G）	0.82	1462.20
RH-7592（H）	0.91	1317.58
					A+B(20：1)	4.01	299.00	165.73	180.42
A+B(15：1)	3.56	336.80	186.27	180.82
					A+B(10：1)	2.07	579.23	225.48	256.89
A+B(5：1)	1.26	951.59	330.04	288.32
					A+B(3：1)	0.77	1557.14	445.06	349.87
A+B(1：1)	0.39	3074.36	790.13	389.10
					A+B(1：3)	0.31	3867.74	1135.19	340.71
A+B(1：5)	0.29	4134.48	1250.21	330.70
					A+B(1：10)	0.32	3746.88	1354.77	276.57
A+B(1：15)	0.44	2725.00	1393.98	195.48
					A+B(1：20)	0.65	1844.62	1414.52	130.41

A+C(20：1)	3.78	317.20	181.75	174.53
					A+C(15：1)	2.87	417.77	207.29	201.54
A+C(10：1)	2.01	596.52	256.06	232.96
					A+C(5：1)	1.17	1024.79	386.11	265.41
A+C(3：1)	0.63	1903.17	529.17	359.65
					A+C(1：1)	0.36	3330.56	958.34	347.54
A+C(1：3)	0.28	4282.14	1387.50	308.62
					A+C(1：5)	0.27	4440.74	1530.56	290.14
A+C(1：10)	0.3	3996.67	1660.61	240.67
					A+C(1：15)	0.33	3633.33	1709.38	212.55
A+C(1：20)	0.41	2924.39	1734.92	168.56
					A+D(20：1)	3.63	330.30	168.44	196.10
A+D(15：1)	2.86	419.23	189.82	220.85
					A+D(10：1)	1.99	602.51	230.65	261.22
A+D(5：1)	1.15	1042.61	339.53	307.07
					A+D(3：1)	0.61	1965.57	459.30	427.95
A+D(1：1)	0.35	3425.71	818.59	418.49
					A+D(1：3)	0.27	4440.74	1177.89	377.01
A+D(1：5)	0.25	4796.00	1297.65	369.59
					A+D(1：10)	0.32	3746.88	1406.53	266.39
A+D(1：15)	0.31	3867.74	1447.36	267.23
					A+D(1：20)	0.42	2854.76	1468.74	194.37
A+E(20：1)	3.64	329.40	170.36	193.35
					A+E(15：1)	2.84	422.18	192.35	219.48
A+E(10：1)	1.96	611.73	234.33	261.06
					A+E(5：1)	1.03	1164.08	346.27	336.17
A+E(3：1)	0.63	1903.17	469.41	405.44
					A+E(1：1)	0.32	3746.88	838.82	446.69
A+E(1：3)	0.26	4611.54	1208.22	381.68
					A+E(1：5)	0.27	4440.74	1331.36	333.55

A+E(1：10)	0.34	3526.47	1443.30	244.33
					A+E(1：15)	0.32	3746.88	1485.28	252.27
A+E(1：20)	0.48	2497.92	1507.27	165.72
					A+F(20：1)	3.29	364.44	188.84	192.99
A+F(15：1)	2.86	419.23	216.60	193.55
					A+F(10：1)	1.97	608.63	269.60	225.76
A+F(5：1)	1.09	1100.00	410.93	267.69
					A+F(3：1)	0.62	1933.87	566.39	341.44
A+F(1：1)	0.28	4282.14	1032.79	414.62
					A+F(1：3)	0.23	5213.04	1499.18	347.73
A+F(1：5)	0.25	4796.00	1654.64	289.85
					A+F(1：10)	0.32	3746.88	1795.97	208.63
A+F(1：15)	0.31	3867.74	1848.97	209.18
					A+F(1：20)	0.42	2854.76	1876.73	152.11
A+G(20：1)	3.31	362.24	164.87	219.71
					A+G(15：1)	2.89	414.88	185.14	224.09
A+G(10：1)	1.95	614.87	223.84	274.70
					A+G(5：1)	1.1	1090.00	327.03	333.30
A+G(3：1)	0.63	1903.17	440.55	432.00
					A+G(1：1)	0.32	3746.88	781.10	479.69
A+G(1：3)	0.27	4440.74	1121.65	395.91
					A+G(1：5)	0.26	4611.54	1235.17	373.35
A+G(1：10)	0.36	3330.56	1338.36	248.85
					A+G(1：15)	0.33	3633.33	1377.06	263.85
A+G(1：20)	0.51	2350.98	1397.33	168.25
					A+H(20：1)	3.42	350.58	157.98	221.92
A+H(15：1)	3.04	394.41	176.10	223.97
					A+H(10：1)	1.86	644.62	210.69	305.96
A+H(5：1)	1.12	1070.54	302.93	353.39
					A+H(3：1)	0.67	1789.55	404.40	442.53

A+H(1：1)	0.4	2997.50	708.79	422.90
					A+H(1：3)	0.24	4995.83	1013.19	493.08
A+H(1：5)	0.28	4282.14	1114.65	384.17
					A+H(1：10)	0.37	3240.54	1206.89	268.50
A+H(1：15)	0.38	3155.26	1241.48	254.15
					A+H(1：20)	0.48	2497.92	1259.60	198.31

The Toxicity Determination of embodiment 3, ZJ5337 and triazole bactericidal agent compounding to rice blast

Rice blast fungus test method is referred to《People's Republic of China (PRC) agricultural industry criteria NY/T1156.2-2006》 Mycelial growth rate method.Rice blast fungus（Pyricularia oryzae）Preserved by this test chamber, and it is identified with compared with Strong is pathogenic.By rice blast fungus PDA culture medium culture, when bacterium colony just covers with culture dish, with the punching of diameter 5mm Device breaks into truffle in colony edge, and truffle is moved to the toxic PDA culture medium central authorities being configured in advance with Inoculating needle, is subsequently placed in Culture in 25 DEG C of incubators, often processes and is repeated 4 times.After 7 days, using crossing method slide calliper rule each process colony diameter is measured Cm, obtains correction inhibition percentage.By the linear regression analyses between the probit value of suppression ratio and the logarithm value of series concentration, Obtain the EC of each medicament₅₀Value, further according to the abundant methods of Sun Yun co-toxicity coefficient is calculated（CTC）.

Drug effect is calculated：Each bacterium colony decussation surveys two diameters, and with its average bacterium colony size is represented.Obtain as the following formula Colony growth suppression ratio：Colony growth suppression ratio %=（Blank bacterium colony increases diameter-chemicals treatment bacterium colony and increases diameter）× 100/ blank bacterium colony increases diameter

Table 3：ZJ5337 compounds the Toxicity Determination result to rice blast with triazole bactericidal agent

Medicament	EC₅₀（μg/mL）	ATI	TTI	Co-toxicity coefficient CTC
					ZJ5337（A）	0.42	100
Tebuconazole（B）	0.28	150.00
					Difenoconazole（C）	0.33	127.27
Hexaconazole（D）	0.22	190.91
					Propiconazole (E)	0.25	168.00
Prothioconazoles（F）	0.30	140.00
					Epoxiconazole（G）	0.23	182.61
RH-7592（H）	0.35	120.00
					A+B(20：1)	0.25	168.00	102.38	164.09

A+B(15：1)	0.23	182.61	103.13	177.08
					A+B(10：1)	0.19	221.05	104.55	211.44
A+B(5：1)	0.15	280.00	108.33	258.46
					A+B(3：1)	0.1	420.00	112.50	373.33
A+B(1：1)	0.08	525.00	125.00	420.00
					A+B(1：3)	0.07	600.00	137.50	436.36
A+B(1：5)	0.08	525.00	141.67	370.59
					A+B(1：10)	0.09	466.67	145.45	320.83
A+B(1：15)	0.15	280.00	146.88	190.64
					A+B(1：20)	0.16	262.50	147.62	177.82
A+C(20：1)	0.24	175.00	101.30	172.76
					A+C(15：1)	0.21	200.00	101.70	196.65
A+C(10：1)	0.18	233.33	102.48	227.69
					A+C(5：1)	0.14	300.00	104.55	286.96
A+C(3：1)	0.12	350.00	106.82	327.66
					A+C(1：1)	0.08	525.00	113.64	462.01
A+C(1：3)	0.09	466.67	120.45	387.43
					A+C(1：5)	0.07	600.00	122.73	488.90
A+C(1：10)	0.11	381.82	124.79	305.97
					A+C(1：15)	0.16	262.50	125.57	209.05
A+C(1：20)	0.19	221.05	125.97	175.48
					A+D(20：1)	0.23	182.61	104.33	175.03
A+D(15：1)	0.2	210.00	105.68	198.71
					A+D(10：1)	0.19	221.05	108.26	204.18
A+D(5：1)	0.11	381.82	115.15	331.58
					A+D(3：1)	0.08	525.00	122.73	427.78
A+D(1：1)	0.06	700.00	145.46	481.25
					A+D(1：3)	0.07	600.00	168.18	356.76
A+D(1：5)	0.08	525.00	175.76	298.71
					A+D(1：10)	0.09	466.67	182.65	255.50

A+D(1：15)	0.11	381.82	185.23	206.13
					A+D(1：20)	0.13	323.08	186.58	173.16
A+E(20：1)	0.22	190.91	103.24	184.92
					A+E(15：1)	0.21	200.00	104.25	191.85
A+E(10：1)	0.15	280.00	106.18	263.70
					A+E(5：1)	0.1	420.00	111.33	377.25
A+E(3：1)	0.08	525.00	117.00	448.72
					A+E(1：1)	0.06	700.00	134.00	522.39
A+E(1：3)	0.08	525.00	151.00	347.68
					A+E(1：5)	0.07	600.00	156.67	382.98
A+E(1：10)	0.08	525.00	161.82	324.44
					A+E(1：15)	0.12	350.00	163.75	213.74
A+E(1：20)	0.13	323.08	164.76	196.09
					A+F(20：1)	0.21	200.00	101.90	196.26
A+F(15：1)	0.2	210.00	102.50	204.88
					A+F(10：1)	0.13	323.08	103.64	311.74
A+F(5：1)	0.1	420.00	106.67	393.75
					A+F(3：1)	0.09	466.67	110.00	424.24
A+F(1：1)	0.07	600.00	120.00	500.00
					A+F(1：3)	0.09	466.67	130.00	358.97
A+F(1：5)	0.08	525.00	133.33	393.75
					A+F(1：10)	0.1	420.00	136.36	308.00
A+F(1：15)	0.12	350.00	137.50	254.55
					A+F(1：20)	0.14	300.00	138.10	217.24
A+G(20：1)	0.2	210.00	103.93	202.05
					A+G(15：1)	0.19	221.05	105.16	210.20
A+G(10：1)	0.12	350.00	107.51	325.55
					A+G(5：1)	0.1	420.00	113.77	369.17
A+G(3：1)	0.07	600.00	120.65	497.30
					A+G(1：1)	0.06	700.00	141.31	495.38

A+G(1：3)	0.06	700.00	161.96	432.21
					A+G(1：5)	0.07	600.00	168.84	355.36
A+G(1：10)	0.08	525.00	175.10	299.83
					A+G(1：15)	0.11	381.82	177.45	215.17
A+G(1：20)	0.12	350.00	178.68	195.89
					A+H(20：1)	0.23	182.61	100.95	180.89
A+H(15：1)	0.17	247.06	101.25	244.01
					A+H(10：1)	0.12	350.00	101.82	343.75
A+H(5：1)	0.11	381.82	103.33	369.50
					A+H(3：1)	0.1	420.00	105.00	400.00
A+H(1：1)	0.08	525.00	110.00	477.27
					A+H(1：3)	0.07	600.00	115.00	521.74
A+H(1：5)	0.1	420.00	116.67	360.00
					A+H(1：10)	0.11	381.82	118.18	323.08
A+H(1：15)	0.13	323.08	118.75	272.06
					A+H(1：20)	0.15	280.00	119.05	235.20

2nd, preparation is prepared

Percentage composition is mass percent in all formulations proportioning.

1st, water dispersible granules

Embodiment 1：60%ZJ5337 tebuconazole water dispersible granules

Weigh 20%ZJ5337,40% Tebuconazole, 3%TERSPERSE2700,2% dispersing agent NNO（Alkylnaphthalene sulfonate formaldehyde Condensation substance）, 3% Nekal BX（Nekal）、2%K-12（Sodium lauryl sulphate）, 3% kieselguhr, 5% glucose, Kaolin adds to 100% mass parts.Above-mentioned raw materials Jing routinely produces the method for water dispersible granules and mixes, ultra micro comminution by gas stream, makes Grain step produces 60%ZJ5337 tebuconazole water dispersible granules.

Embodiment 2：50%ZJ5337 difenoconazole water dispersible granules

Weigh 20%ZJ5337,30% Difenoconazole, 3%TERSPERSE2700,3% dispersing agent NNO, 2% Nekal BX, 1% K-12,1% carboxymethyl cellulose, 3% carbamide, kieselguhr add to 100% mass parts.Above-mentioned raw materials Jing routinely produces water dispersible granules Method mix, ultra micro comminution by gas stream, granulation step produce 50%ZJ5337 difenoconazole water dispersible granules.

Embodiment 3：80%ZJ5337 diniconazole water dispersible granules

Weigh 40%ZJ5337,40% hexaconazole, 4%TERSPERSE2700,2% dispersing agent NNO, 2% Nekal BX, 1%K- 12nd, 3% corn starch, 3% sulphur ammonium, attapulgite add to 100% mass parts.Above-mentioned raw materials Jing routinely produces the side of water dispersible granules Method mixes, ultra micro comminution by gas stream, granulation step produce 80%ZJ5337 diniconazole water dispersible granules.

2nd, suspending agent

Embodiment 4：40%ZJ5337 tebuconazole suspension concentrates

Weigh 15%ZJ5337,25% Tebuconazole, 2%TERSPERSE2500,3%TERSPERSE2425,0.2% xanthan gum, 3% White carbon, 5% ethylene glycol, 0.3% benzoic acid, 0.5% organic silicon defoamer（Trade name：S-29 Nanjing "four news" (new ideas applied chemistry product company Produce）, deionized water adds to 100% mass parts.Above-mentioned raw materials are blended, high speed shear dispersion 30min, after sand mill sand milling Prepared 40%ZJ5337 tebuconazole suspension concentrates.

Embodiment 5：35%ZJ5337 difenoconazole suspending agent

Weigh 15%ZJ5337,20% Difenoconazole, 2%NNO, 2%TERSPERSE2500,0.1% xanthan gum, 3% Linesless charcoal Black, 5% Propylene Glycol, 0.5% formaldehyde, 0.5% organic silicon defoamer, deionized water adds to 100% mass parts.Above-mentioned raw materials are blended, High speed shear disperses 30min, with prepared 35%ZJ5337 difenoconazole suspending agent after sand mill sand milling.

Embodiment 6：25%ZJ5337 epoxiconazole suspension concentrates

Weigh 15%ZJ5337,10% epoxiconazole, 1%TERSPERSE2500,1%TERSPERSE2425,0.2% xanthan gum, 3% White carbon, 5% ethylene glycol, 0.3% benzoic acid, 0.5% organic silicon defoamer, deionized water adds to 100% mass parts.Above-mentioned raw materials Jing Mixing, high speed shear dispersion 30min, with prepared 25%ZJ5337 epoxiconazole suspension concentrates after sand mill sand milling.

3rd, wettable powder

Embodiment 7：80%ZJ5337 Tebuconazole wettable powders

Weigh 30%ZJ5337,50% Tebuconazole, 5% calcium lignosulfonate, 2% Nekal BX, 1%K-12,5% white carbon, height Age soil adds to 100% mass parts.Above-mentioned raw materials are blended, and 80%ZJ5337 Tebuconazole wettable powders are obtained after comminution by gas stream. Embodiment 8：65%ZJ5337 Difenoconazole wettable powders

Weigh 60%ZJ5337,5% Difenoconazole, 4% calcium lignosulfonate, 3%TERSPERSE2700,2% Nekal BX, 2%K-12,5% white carbon, fine particle calcium carbonate add to 100% mass parts.Above-mentioned raw materials are blended, and 65%ZJ5337 benzene is obtained after comminution by gas stream Ether methyl cyclic-azole wettable powder.

Embodiment 9：52%ZJ5337 RH-7592 wettable powders

Weigh 4%ZJ5337,48% RH-7592,4% calcium lignosulfonate, 4% dispersing agent NNO, 2% Nekal BX, 2%K-12, 5% white carbon, attapulgite add to 100% mass parts.Above-mentioned raw materials are blended, and 52%ZJ5337 nitrile benzene is obtained after comminution by gas stream Azoles wettable powder.

4th, microemulsion

Embodiment 10：15%ZJ5337 tebuconazole microemulsions

Weigh 5%ZJ5337,10% Tebuconazole, 4%TX-10,5% Nongru-700 #, 6% agriculture breast 500#, 4% agriculture breast 1601#, 15% Cyclohexanone, 5%N- methyl pyrrolidones, 5% n-butyl alcohol, 1% epoxychloropropane, the complete simultaneously mix homogeneously of Jing dissolvings, deionized water adds To 100% mass parts, 15%ZJ5337 tebuconazole microemulsions are obtained after stirring.

Embodiment 11：25%ZJ5337 micro-emulsion of phenyl ether methyl cyclic-azole

Weigh 10%ZJ5337,15% Difenoconazole, 5% agriculture breast 400#, 4% agriculture breast 500#, 6% agriculture breast 1601#, 25% ring Ketone, 5% dimethylbenzene, 5% isopropanol, 3% triphenyl phosphite, the complete simultaneously mix homogeneously of Jing dissolvings, deionized water adds to 100% mass Part, 25%ZJ5337 micro-emulsion of phenyl ether methyl cyclic-azole is obtained after stirring.

Embodiment 12：15%ZJ5337 azoles alcohol microemulsions

Weigh 10%ZJ5337,5% azoles alcohol, 6% agriculture breast 2201#, 5% Nongru-700 #, 6% agriculture breast 600#, 10% cyclohexanone, 10%N- methyl pyrrolidones, 5% toluene, 5% ethanol, 1% epoxychloropropane, the complete simultaneously mix homogeneously of Jing dissolvings, deionized water adds To 100% mass parts, 15%ZJ5337 azoles alcohol microemulsions are obtained after stirring.

Embodiment 13：40%ZJ5337 propiconazole microemulsions

Weigh 15%ZJ5337,25% propiconazole, 4%TX-10,8% agriculture breast 600#, 6% agriculture breast 500#, 20% cyclohexanone, 5%N- Methyl pyrrolidone, 10% toluene, 1% epoxychloropropane, the complete simultaneously mix homogeneously of Jing dissolvings, deionized water adds to 100% mass Part, 40%ZJ5337 propiconazole microemulsions are obtained after stirring.

5th, aqueous emulsion

Embodiment 14：15%ZJ5337 prothioconazoles aqueous emulsions

Weigh 10%ZJ5337,5% prothioconazoles, 3% agriculture breast 2201#, 1.5% Nonyl pheno（EO=10）Ether phosphoric acid Ester, 2.5% triphenoethyl benzene phenol polyethenoxy ether phosphate（Agriculture breast 600# acid phospholipid）, 2%Span-60#, 1% epoxychloropropane, 20% dimethylbenzene, 10% Ketohexamethylene, 0.3% xanthan gum, 0.5% benzoic acid, deionized water adds to 100% mass parts.Above-mentioned raw materials Jing is mixed Close, high speed shearing emulsification is obtained 15%ZJ5337 prothioconazoles aqueous emulsions.

Embodiment 15：16%ZJ5337 difenoconazole water emulsions

Weigh 6%ZJ5337,10% Difenoconazole, 2% agriculture breast 2201#, 3% Nonyl pheno（EO=10）Ether phosphoric acid Ester, 2%Span-60#, 2% emulsifying agent T-60,1% triphenyl phosphite, 15% dimethylbenzene, 10% Ketohexamethylene, 0.3% xanthan gum, 0.5% Benzoic acid, deionized water adds to 100% mass parts.Above-mentioned raw materials are blended, and high speed shearing emulsification is obtained 16%ZJ5337 phenylates Methyl cyclic-azole aqueous emulsion.

Embodiment 16：15%ZJ5337 hexaconazole aqueous emulsions

Weigh 10%ZJ5337,5% hexaconazole, 2.5% Nonyl pheno（EO=10）Ether phosphate, 2.5% 3 phenethyl Phenol polyoxyethylene phosphonate, 2%Span-60#, 1% epoxychloropropane, 10% dimethylbenzene, 20% Ketohexamethylene, 0.3% xanthan gum, 0.5% benzoic acid, deionized water adds to 100% mass parts.Above-mentioned raw materials are blended, and high speed shearing emulsification is obtained 15%ZJ5337 Hexaconazole aqueous emulsion.

Embodiment 17：30%ZJ5337 propiconazole emusion in water

Weigh 10%ZJ5337,20% propiconazole, 2% agriculture breast 2201#, 3.5% triphenoethyl benzene phenol polyethenoxy ether phosphate, 3%Span-60#, 1% epoxychloropropane, 30% dimethylbenzene, 0.3% xanthan gum, 0.5% benzoic acid, deionized water adds to 100% mass Part.Above-mentioned raw materials are blended, and high speed shearing emulsification is obtained 30%ZJ5337 propiconazole emusion in water.

Embodiment 18：5%ZJ5337 RH-7592 aqueous emulsions

Weigh 1%ZJ5337,4% RH-7592,2% agriculture breast 2201#, 3.5% triphenoethyl benzene phenol polyethenoxy ether phosphate, 3% Span-60#, 1% epoxychloropropane, 30% dimethylbenzene, 0.3% xanthan gum, 0.5% benzoic acid, deionized water adds to 100% mass parts. Above-mentioned raw materials are blended, and high speed shearing emulsification is obtained 5%ZJ5337 RH-7592 aqueous emulsions.

3rd, Field information

Embodiment 1, prevent and treat rice sheath blight disease field control effectiveness test

Rice sheath blight disease field control effectiveness test is prevented and treated with reference to medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion（One） GB/T17980.20-2000 bactericidal agent for preventing and treating rice sheath blight diseases " carry out field control effectiveness test.Test medicine, comparison medicament and sky The cell of white control is processed using the arrangement of random district's groups, plot area 25m2,4 repetitions, the common dispenser of this test twice, during dispenser Oryza sativa L. is in full heading time, and rice sheath blight disease is in their early stage（Radix before medicine is not investigated）.Adjust within 7 days 7 days after 1 medicine, after 2 medicines Look into result of the test.Adopt 5 points of samplings of diagonal, per investigation to be connected 5 clumps per experimental plot, 25 clumps are investigated altogether, investigation per clump is every Strain incidence, records disease grade.

1）Disease scale standard is as follows：

0 grade：It is disease-free；

1 grade：Quaterfoil and its following sheath, blade are fallen ill (with sword-like leave as first piece leaf)；

3 grades：Third blade and its following sheath, blade morbidity；

5 grades：Second blade and its following sheath, blade morbidity；

7 grades：Sword-like leave blade and its following folic acid, blade morbidity；

9 grades：Herb falls ill, withered ahead of time.

2）Drug effect computational methods：

According to survey result, according to following（1）、（2）Formula calculates disease index and preventive effect.Test data adopts Deng Kenshi Duncan's new multiple range method（DMRT）Carry out statistical analysiss.

Disease index=∑（The sick numbers of sheets at different levels × relative value of series）*100/（Investigation sum × 9）……（1）

Prevention effect %=（CK1-PK1）*100/CK1……（2）.

In formula：CK1 --- disease index after blank district dispenser；PT1 --- disease index after chemicals treatment district dispenser.

Table 4：Prevent and treat rice sheath blight disease field control effectiveness test result

Embodiment 2, preventing and treating rice green smut field control effectiveness test

Preventing and treating rice green smut field control effectiveness test is with reference to medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion（One） GB/T17980.19-2000 bactericidal agent for preventing and treating rice green smuts " carry out field control effectiveness test.Test medicine, comparison medicament and sky The cell of white control is processed using the arrangement of random district's groups, plot area 25m2,4 repetitions, common dispenser twice, during first time dispenser Oryza sativa L.（Kind is peaceful 81）In Oryza sativa L. cut early stage, Oryza sativa L. is investigated and presses " pesticide field efficacy in the cut phase during second dispenser Test rule " is carried out, and using parallel saltatory five point sampling, 25 clumps of rice is investigated per cell, investigates total spike number and false smut is sent out State of an illness condition, records disease grade, calculates disease tassel yield, disease index and prevention effect.This is tested in stage of wax ripeness（35 days after 2 medicines） Investigation 1 time, does not investigate radix before medicine.

1）Disease scale standard is as follows（In units of fringe）：

0 grade：Without infected seed；

1 grade：Every 1 curve of fringe or the grain of rice of catching an illness；

3 grades：Every 2 curves of fringe or the grain of rice of catching an illness；

5 grades：Every 3-5 curve of fringe or the grain of rice of catching an illness；

7 grades：Every 6-9 curve of fringe or the grain of rice of catching an illness；

9 grades：More than per fringe 10 curves or the grain of rice of catching an illness.

2）Drug effect computational methods

Table 5：Preventing and treating rice green smut field test results

Embodiment 3, preventing and treating rice blast field control effectiveness test

Preventing and treating rice blast field control effectiveness test is with reference to medicine inspecting institute of the Ministry of Agriculture " GB-T 17980.19-2000 pesticide field Test of pesticide effectiveness criterion（One）--- bactericidal agent for preventing and treating Oryza sativa L. leaf diseases " regulation, test medicine, comparison medicament and blank Cell process using random district's groups arrangement, plot area 25m², 4 repetitions, this test dispenser first time dispenser twice altogether When Oryza sativa L. be in the Oryza sativa L. cut phase, during second dispenser Oryza sativa L. in full heading time, rice blast is mainly panicle blast, in morbidity Initial stage.This test is investigated 2 times altogether, and per experimental plot using the 5 points of samplings of parallel saltatory, per 50 fringes of investigation investigate altogether 250 Fringe, investigates per clump per fringe incidence, records disease grade.Drug effect computational methods are with Field information example 1.

1）Disease scale standard is as follows：

0 grade：It is disease-free；

1 grade：The loss less than 5% per fringe（Indivedual branch stalk morbidities）；

3 grades：The loss 6%～20% per fringe（The stalk morbidity of 1/3rd or so branches）；

5 grades：The loss 21%～50% per fringe（Fringe neck or main shaft are fallen ill, and grain half is flat）；

7 grades：The loss 51%～70% per fringe（Fringe neck is fallen ill, and big portion half is flat）；

9 grades：The loss 71%～100% per fringe（Fringe neck is fallen ill, and causes dead ears）.

2）Drug effect computational methods

In formula：CK0, CK1 are respectively the forward and backward disease index of blank district dispenser；PT0, PT1 are respectively chemicals treatment The forward and backward disease index of area's medicine.

Table:6：Preventing and treating rice blast field test results

Embodiment 4, prevent and treat Hemintho-sporum leaf spot of rice plants field control effectiveness test

Hemintho-sporum leaf spot of rice plants field control effectiveness test is prevented and treated with reference to medicine inspecting institute of the Ministry of Agriculture " GB-T 17980.19-2000 pesticide field Between test of pesticide effectiveness criterion（One）--- bactericidal agent for preventing and treating Oryza sativa L. leaf diseases " regulation, test medicine, comparison medicament and blank are right According to cell process using random district's groups arrangement, plot area 25m², 4 repetitions, altogether dispenser is applied for the first time twice for this test Helminthosporium leaf spot is in their early stage during medicine.This test is investigated 2 times altogether, per experimental plot using the 5 points of samplings of parallel saltatory, often 50 plants of point investigation, per plant of investigation boot leaf and the following two panels leaf of boot leaf, investigate incidence, record disease etc. in units of blade Level.Drug effect computational methods are with Field information example 3.

1）Disease scale standard is as follows：

0 grade：It is disease-free；

1 grade：Lesion area accounts for less than the 1% of full wafer leaf area；

3 grades：Lesion area accounts for less than the 2%～5% of full wafer leaf area；

5 grades：Lesion area accounts for less than the 6%～15% of full wafer leaf area；

7 grades：Lesion area accounts for less than the 16%～25% of full wafer leaf area；

9 grades：Lesion area accounts for more than the 25% of full wafer leaf area, and sheath is typically withered.

2）Drug effect computational methods are with Field information example 3.

Table:7：Prevent and treat Hemintho-sporum leaf spot of rice plants field test results

Can be seen that from above effect data：The bactericidal composition that the present invention is provided can be used to prevent and treat rice disease, especially There is specially good effect to rice sheath blight disease, rice blast, false smut and Hemintho-sporum leaf spot of rice plants, rationally, treatment adds protective effect, sterilization effect to component It is really good, times for spraying is reduced, drug cost is low, and its activity and bactericidal effect are not the simple superposition of each component activity, but There is significant potentiation, slow down the generation of resistance, to crop safety, meet the security requirement of pesticidal preparations.

Claims

1. a kind of bactericidal composition, it is characterised in that the bactericidal composition contains carbonic acid benzyl ester -2, the fluorine of 3,8- trimethyl -6- seven Isopropyl -4- quinoline ester and triazole bactericidal agent, the triazole bactericidal agent is selected from hexaconazole, propiconazole, prothioconazoles, fluorine ring One kind, two or three combination of the above in azoles and RH-7592, carbonic acid benzyl ester -2, the fluorine isopropyls of 3,8- trimethyl -6- seven - 4- quinoline ester is 20 with the mass ratio of triazole bactericidal agent:1～1:20.

2. according to the bactericidal composition described in claim 1, it is characterised in that carbonic acid benzyl ester -2,3,8- trimethyl -6- seven Fluorine isopropyl -4- quinoline ester is 10 with the mass ratio of triazole bactericidal agent:1～1:10.

3. according to the bactericidal composition described in claim 1, it is characterised in that the triazole bactericidal agent is selected from propiconazole, rosickyite One kind in bacterium azoles, RH-7592, two or three.

4. according to the bactericidal composition described in claim 1, it is characterised in that the bactericidal composition is formulated into antibacterial, institute State antibacterial and contain acceptable carrier on bactericidal composition and pesticide, the bactericidal composition accounts for the 1% of antibacterial gross weight ～80%.

5. according to the bactericidal composition described in claim 4, it is characterised in that the bactericidal composition accounts for antibacterial gross weight 5%～80%.

6. according to the bactericidal composition described in claim 4, it is characterised in that the antibacterial is formulated into water dispersible granules, hangs Floating agent, wettable powder, aqueous emulsion or microemulsion.

7. according to the bactericidal composition described in one of claim 1-6, it is characterised in that the bactericidal composition is used for preventing and treating Rice sheath blight disease, rice blast, false smut or Hemintho-sporum leaf spot of rice plants.

8. according to the bactericidal composition described in claim 6, it is characterised in that each composition quality proportioning in the water dispersible granules For：Bactericidal composition 5～80%, dispersant 3～12%, wetting agent 1-8%, disintegrating agent 1～10%, binding agent 0～8%, its It is remaining for filler；Each composition quality proportioning is in the wettable powder：Bactericidal composition 5～80%, dispersant 5～10%, profit Humectant 0-10%, remaining be filler.