CN108477199A - A kind of bactericidal composition and application thereof - Google Patents
A kind of bactericidal composition and application thereof Download PDFInfo
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- CN108477199A CN108477199A CN201810481626.7A CN201810481626A CN108477199A CN 108477199 A CN108477199 A CN 108477199A CN 201810481626 A CN201810481626 A CN 201810481626A CN 108477199 A CN108477199 A CN 108477199A
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- biguanides
- trifloxystrobin
- benzene sulfonate
- fungicide
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
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Abstract
The present invention provides a kind of bactericidal compositions and application thereof.The main active of the bactericidal composition includes two kinds of components, component one：Three octyl benzene sulfonate of biguanides；Component two：Xinjunan acetate, 2 carboxylic acids of azophenlyene α, prothioconazoles or thiophene trifloxystrobin, wherein the mass ratio of component one and component two is 1:20~20:1, the percentage that two kinds of component gross masses account for composition gross mass is 5%~80%.The composition is reduced synergy, toxicity is low, small to people and animals and ecological environment harm, while can delay the generation and development of pathogen drug resistance, there is preferable control effect to scab, powdery mildew, black spot, brown spot fungal disease, has broad application prospects.
Description
Technical field
The present invention relates to fungicide fields, specifically, being related to one kind containing three octyl benzene sulfonate (component of biguanides
One) and Xinjunan acetate, azophenlyene α -2 carboxylic acids, prothioconazoles or thiophene trifloxystrobin (component two) bactericidal composition.
Background technology
Three octyl benzene sulfonate of biguanides, chemical name：Three octyl phenylbenzimidazole sulfonic acid salt of 1,1- octyl methylene biguanides.It is double
Three octyl benzene sulfonate of guanidine is wide spectrum, less toxic fungicide, inhibits germ quasi-grease compound biosynthesis, local permeability
By force, there is very high growth inhibitory activity to pathogen sac fungus, Fungi Imperfecti.
Xinjunan acetate is quaternary ammonium salt fungicide, and cell membrane, coagulated protein, prevention are obtained by destroying all kinds of pathogen
The modes such as breathing and ferment activity reach sterilization purpose.With certain interior suction and osmosis, to leading to the more of crop disease
Kind plant epiphyte, bacterium and virus have significant killing and inhibiting effect.
Azophenlyene α -2 carboxylic acids can dissolve rapidly harmful fungoid, bacterium cell wall, pathogenic bacteria are directly killed, and generates
Cell antibody interferes the synthesis of germ cell wall, inhibits the generation of disease, develops；Harmful levels of pathogens is killed, dissolving germ secretion
Toxin dredges plant microcirculation, the conveying of unimpeded nutriment；Plant is induced to generate disease resistance, disease resistance is formed powerful
Defense response system is effective to a variety of fruit tree fungal diseases.
Not only there is prothioconazoles good systemic action, excellent protection to treat and root out activity, and lasting period
It is long.Compared with triazole bactericidal agent, prothioconazoles have wider fungicidal spectrum.
Thiophene trifloxystrobin is a kind of pharmaceutical preparations of specific treatment powdery mildew, and nanometer DV technologies and targeting pharmaceutical technology are combined
So that thiophene trifloxystrobin is improved 5 times than trifloxystrobin sterilization intensity, 10 times are improved than pyraclostrobin；The medicament has superpower penetration,
Fast, the features such as conduction is rapid, and the lasting period is long is absorbed, in powdery mildew to pyraclostrobin, ether sulfocarbolate, kresoxim-methyl, mostly anti-
Mycin, nitrile bacterium azoles etc. generate the area of resistance, and using effect is clearly；This product is all very safe to various crop, and the florescence is available,
Fruit is not polluted, natural enemy is not injured.
Pear scab is called shot hole, and main pears area of China occurs generally, and popular strong, loss is big, always from the fallen flowers phase
Cause harm to fructescence.Aggrieved young fruit is malformed fruit, growth stage fruit suberification.Blade is aggrieved, extended along vein to be formed it is black
The mildew even mould layer of black.Petiole, carpopodium symptom are similar, the recess spot of Black oval occur, and sick portion covers black mould, contracting of hanging,
Dehydration is withered, causes blade or fruit caducous.
Pears powdery mildew is a kind of fungal disease that pears producing region occurs, and a large amount of white powder is presented when disease occurs on blade
Shape spot influences the plant strain growth of second year when occurring serious.This sick worldwide generally existing, especially coastal state
Family and area occur in the main areas Chan Li of east China than more serious.
Pear tree black spot is one of the important disease on pear tree, is generally occurred in Chinese main pears area.European pear, Japan
Pears, crisp pears, Chinese pear are most easily susceptible, early leaf fall and tender tip can be caused withered when falling ill serious, cause dehiscent fruit and early stage shedding,
Tree vigo(u)r is seriously undermined, the growth potential and disease resistance of tree body are reduced.
Pears brown spot is also known as pears leaf pinta, pears spot blight, and mainly cause harm blade and fruit cause pears leaf dried-up when serious,
A large amount of fallen leaves.
Invention content
The object of the present invention is to provide a kind of decrement synergy, pathogen drug resistance is delayed to generate and develop, reduction pathogen
The risk of resistance is generated to single bactericide, extends the bactericidal composition of fungicide service life.
In order to realize the object of the invention, the present invention provides a kind of bactericidal compositions, include mainly two kinds of active constituents：
Component one：Three octyl benzene sulfonate of biguanides；
Component two：Any one of Xinjunan acetate, azophenlyene α -2 carboxylic acids, prothioconazoles, thiophene trifloxystrobin are several.
Further, the mass ratio of component one and component two is 1 in the bactericidal composition:20~20:1；Preferably 1:
15~15:1, further preferably 1:10~10:1, more preferably 1:8~8:1, it is still more preferably 1:5~5:1, preferably
It is 1:1.For example, the mass ratio of component one and component two can be 1:20、20:1、1:15、15:1、1:10、10:1、1:8、8:1、1:
5、5:1 or 1:1.
The present invention also provides a kind of fungicide, can be separately made by above-mentioned bactericidal composition, also can be by above-mentioned sterilization group
Object is closed to be made with the available auxiliary agent of pesticide field or carrier.
The auxiliary agent includes dispersant, lubricant, binder, dispersant, spreader-sticker, disintegrant, antifoaming agent, emulsification
One or more of agent, wetting agent, thickener, preservative, stabilizer, antifreezing agent or cosolvent.
The fungicide can be made into the available various dosage forms of pesticide field, preferably water dispersible granules, aqueous emulsion or micro emulsion
Agent.
Further, in the fungicide, the gross mass of the component one and component two account for fungicide gross mass 5%~
80%, preferably 20%~60%.
When the fungicide is water dispersible granules, it may include one or more of following auxiliary agent：A, dispersant：
Polycarboxylate, lignosulfonates, APES formaldehyde condensation products sulfat, alkyl benzene sulfonate calcium salt, naphthalene sulfonic acids
Formaldehyde condensation products sodium salt, alkyl phenol polyoxyethylene ether, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, glycerine fatty acid
It is one or more in ester APEO；B, wetting agent：Lauryl sodium sulfate, calcium dodecyl benzene sulfonate, Nekal BX,
In wetting and penetrating agent F, alkylnaphthalene sulfonate, polyoxyethylene tristyrene phenyl phosphate, spaonin powder, silkworm excrement, spaonin powder
It is one or more；C, disintegrant：Bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, sodium bicarbonate, lightweight carbonic acid
It is one or more in calcium；D, thickener is：Xanthans, hydroxymethyl cellulose, hydroxyethyl cellulose, methylcellulose, silicic acid
It is one or more in magnalium, polyvinyl alcohol, cornstarch；E, diffusant：NN0.
When the fungicide is aqueous emulsion, it may include one or more of following auxiliary agent：A, emulsifier：Nonyl
Phenol polyethenoxy (EO=10) ether phosphate, triphenoethyl benzene phenol polyethenoxy ether phosphate, phenethyl phenol polyethenoxy ether phosphorus
Acid esters, alkyl phenol polyoxyethylene ether, Nongru-700 # (common names：Alkyl phenol formaldehyde resin polyoxyethylene ether), agriculture breast 2201#, this
Disk -60# (common names：Span60), Tween-60 # (common names：Polyoxyethylene sorbitan stearic acid
Ester), TX-10 (common names：Octylphenol polyethylene ethylene oxide (10) ether), 1601 (common name of agriculture breast：Triphenoethyl benzene phenol polyoxypropylene is poly-
Ethylene oxide block polymer), it is agriculture breast 600#, one or more in agriculture breast 400#；B, solvent：It is dimethylbenzene, toluene, cyclohexanone, molten
Agent oil is one or more；C, stabilizer：It is one or more in triphenyl phosphite, epoxychloropropane, epoxidized soybean oil；D, antifreeze
Agent：It is one or more in ethylene glycol, propylene glycol, glycerine, urea, inorganic salts such as sodium chloride；E, antifoaming agent：From silicone oil, silicone
Class compound, C10-20 saturated fat acid compounds, C8-10 fatty alcohols it is one or more.F, thickener：Xanthans gathers
It is one or more in vinyl alcohol, bentonite, aluminium-magnesium silicate；G, preservative：It is one or more in formaldehyde, benzoic acid, sodium benzoate,
Water is deionized water.
When the fungicide is microemulsion, it may include one or more of following auxiliary agent：A, emulsifier：12
Alkyl benzene calcium sulfonate and aliphatic acid polyethenoxy ether, APES sulfosuccinate, styrylphenol polyoxy
Vinethene, nonylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, aliphatic acid polyethenoxy base ester, polyoxyethylene aliphatic alcohol ether
Any one of or more than one；B, antifreezing agent：It is one or more in ethylene glycol, propylene glycol, glycerine；C, stabilizer：
One kind in sodium citrate, resorcinol；D, solvent：Xylene or biodiesel, toluene, diesel oil, methanol, ethyl alcohol, n-butanol,
Isopropanol, turpentine-based vegetable oil code name be one kind in ND-45, solvent naphtha, dimethylformamide, dimethyl sulfoxide (DMSO), water etc. or
More than one, water is deionized water；E, antifreezing agent：It is a kind of in ethylene glycol, propylene glycol, glycerine, urea, inorganic salts such as sodium chloride
Or it is a variety of；F, antifoaming agent：From silicone oil, silicone compound, C10-20 saturated fat acid compounds, C8-10 fatty alcohols one
Kind is a variety of；G, thickener：Xanthans, carboxymethyl cellulose, carboxyethyl cellulose, methylcellulose, aluminum magnesium silicate, polyethylene
It is one or more in alcohol.
Specifically, the fungicide is water dispersible granules, including three octyl benzene sulfonate of 6-35% biguanides, 1.8-54%
The component two, 1-5% alkyl phenol polyoxyethylene ether, 1-3% Negels, 5-10% lignosulfonates, 1-3% wood
Quality sodium sulfonate, 1-3% lauryl sodium sulfate, 1-5% neopelexes, 5-10%NN0,10-15% urea, 1-
3% magnesium chloride, 1-5% precipitated calcium carbonates, 1-3% starch, cornstarch or ammonium sulfate complement to 100%.
Specifically, the fungicide is aqueous emulsion, including component two, 1-5% benzene described in 5-10% thiophenes trifloxystrobin, 1-15%
Ethyl phenol polyoxyethylene ether phosphate, 1-3% alkyl phenol polyoxyethylene ether, 1-3% agriculture breasts 33#, 10-15% solvent naphtha, Jia Shui
Complement to 100%.
Specifically, the fungicide is microemulsion, including component two, 1-10% described in 5-30% thiophenes trifloxystrobin, 5-20%
Calcium dodecyl benzene sulfonate, 1-5% neopelexes, 1-5% phenethyl phenol polyethenoxy ethers phosphate, 1-3% alkane
Base phenol polyethenoxy ether, 1-3% agriculture breasts 33#, 10-15% solvent naphtha, 10% propylene glycol or 10% isopropanol, Jia Shui are complemented to
100%.
Three octyl benzene sulfonate of biguanides, sterling is brown solid.Its mode of action is that the biology of germ lipoid is inhibited to close
At being a kind of fungicide of broad spectrum activity, local permeability is strong, there is very high growth inhibitory activity to certain disease fungus.
Xinjunan acetate is quaternary ammonium salt fungicide, and sterling is colourless aqueous liquid.It is obtained by destroying all kinds of pathogen
Cell membrane, prevents the modes such as breathing and ferment activity from reaching sterilization at coagulated protein, has interior suction and osmosis, to causing to make
Various plants fungi, bacterium and the virus of object disease have significant killing and inhibiting effect.
Azophenlyene α -2 carboxylic acid sterlings are white or micro-yellow powder, are combined using nanometer DV technologies and targeting pharmaceutical technology,
It is effective to various crop disease, especially powdery mildew.
Prothioconazoles are sterol demethylation (DMI) or ergosterol biosynthesis inhibitor, and sterling is white or light gray
Brown powder crystallizes.With good systemic action, excellent protection, activity is treated and roots out, the lasting period is long.
Thiophene trifloxystrobin is a kind of pharmaceutical preparations of specific treatment powdery mildew, and sterling is colourless or yellowish oily liquids.It
It is a kind of new product developed again on the basis of trifloxystrobin, uses nanometer DV technologies and ground with what targeting pharmaceutical technology was combined
Method processed, 5 times are improved than trifloxystrobin sterilization intensity, 10 times are improved than pyraclostrobin, in powdery mildew to pyraclostrobin, ether
Sulfocarbolate, kresoxim-methyl, polyoxin, nitrile bacterium azoles etc. generate the area of resistance, and using effect is apparent.
The invention also includes the purposes of above-mentioned bactericidal composition or above-mentioned fungicide on crop disease control,
The disease includes the fungal diseases such as scab, powdery mildew, black spot, brown spot.
The crops include pears apple, grape, peach, plum, apricot, cherry, citrus.
The raw materials used in the present invention is commercially available to buy, or is prepared by this field conventional method.
On the basis of common knowledge of the art, above-mentioned each optimum condition can be combined with each other each preferably to get the present invention
Example.
Furthermore, it is to be understood that herein, also included in the meaning of terms such as "include", "comprise", " containing " " by ...
Composition ", " by ... constitute ", " by ... it is made " etc..
Agricultural bactericidal composition bioactivity provided by the present invention is high, and dosage is few on unit area, and dispenser cost is low,
Simultaneously because three octyl benzene sulfonate of biguanides and Xinjunan acetate, azophenlyene α -2 carboxylic acids, prothioconazoles in the present composition
Or thiophene trifloxystrobin belongs to low toxicity, therefore small, environment-friendly, beneficial organism, Environmental security are endangered to people and animals.Into
One step, the generation and development of pathogen drug resistance can also be delayed on the basis of bactericidal composition synergy of the present invention, dropped
Low pathogen generates single bactericide the risk of resistance, extends the service life of each component in bactericidal composition.Further,
There is very high control effect to pear scab, black spot, brown spot, powdery mildew fungal disease, improves the yield and matter of pears
Amount.
Specific implementation mode
The auxiliary agent used in the composition of the present invention includes dispersant, emulsifier, dispersant, antifreeze, thickener, wetting
Agent etc. and it is other be beneficial to active ingredient in the formulation stablize and drug effect play known substance, be all in pesticidal preparations commonly use or
Allow the various composition used, be not particularly limited, specific ingredient and dosage are determined according to recipe requirements by simple experiment.
Following embodiment is only enumerated the proportioning of fraction compositions, and however, it is not limited to this.
Embodiment 1
Weigh 30% biguanides, three octyl benzene sulfonate, 1.8% Xinjunan acetate, 4% alkyl phenol polyoxyethylene ether,
10%NN0,15% urea, cornstarch add to 100%, and above-mentioned each component is sufficiently mixed crushing, is granulated after fluidized drying
The three octyl benzene sulfonate Xinjunan acetate water dispersible granules containing 31.8% biguanides are made.
Embodiment 2
Weigh 6% biguanides, three octyl benzene sulfonate, 54% azophenlyene α -2 carboxylic acids, 2% Negel, 1.5% wooden
Plain sodium sulfonate, 2% lauryl sodium sulfate, ammonium sulfate complement to 100%.By active constituent, dispersant, wetting agent and disintegrant
It is mixed according to the formula uniformly, through air-flow crushing at wettable powder, adds a certain amount of water mixing extruding and make material, through dry
60% biguanides, three octyl benzene sulfonate azophenlyene α -2 carboxylic acid water dispersible granules are obtained after dry screening.
Embodiment 3
Weigh 35% biguanides, three octyl benzene sulfonate, 45% prothioconazoles, 6% lignosulfonates, 4% dodecyl
Benzene sulfonic acid sodium salt, 3% magnesium chloride, 2% starch, 5% precipitated calcium carbonate are mixed to prepare 80% biguanides, three octyl benzene sulfonate third
Sulphur bacterium azoles water dispersible granules.
Embodiment 4
Weigh 5% biguanides, three octyl benzene sulfonate, 15% thiophene trifloxystrobin, 3% phenethyl phenol polyethenoxy ether phosphate,
2% alkyl phenol polyoxyethylene ether, 13% solvent naphtha, 5% ethylene glycol add water to 100%.It is configured to according to conventional formulation method
Active constituent content is 20% biguanides, three octyl benzene sulfonate thiophene trifloxystrobin water emulsion.
Embodiment 5
Weigh 5% biguanides, three octyl benzene sulfonate, 1% Xinjunan acetate, 3% phenethyl phenol polyethenoxy ether phosphoric acid
Ester, 2% agriculture breast 33#, 1% alkyl phenol polyoxyethylene ether, 13% solvent naphtha add water to 100%.The above raw material is routinely prepared
The method input mixing kettle high speed mixing of aqueous emulsion, it is 6% biguanides, three octyl benzene sulfonate that active constituent content, which is made,
Xinjunan acetate aqueous emulsion.
Embodiment 6
Weigh 10% biguanides, three octyl benzene sulfonate, 5% prothioconazoles, 3% phenethyl phenol polyethenoxy ether phosphate,
2% agriculture breast 33#, 1% alkyl phenol polyoxyethylene ether, 13% solvent naphtha add water to 100%.The above raw material is routinely prepared into water
The method input mixing kettle high speed mixing of emulsion, it is 15% biguanides, three octyl benzene sulfonic acid that principle active component content, which is made,
Salt prothioconazoles aqueous emulsion.
Embodiment 7
Weigh 5% biguanides, three octyl benzene sulfonate, 5% azophenlyene α -2 carboxylic acids, 6% calcium dodecyl benzene sulfonate, 3%0
Dialkyl benzene sulfonic acids sodium, 1% alkyl phenol polyoxyethylene ether, 15% solvent naphtha, 10% propylene glycol, add water to 100%.According to routine
Formulation method be configured to active constituent content be 10% biguanides, three octyl benzene sulfonate azophenlyene α -2 carboxylic acid microemulsions.
Embodiment 8
Weigh 10% biguanides, three octyl benzene sulfonate, 20% prothioconazoles, 2% calcium dodecyl benzene sulfonate, 3% 12
Sodium alkyl benzene sulfonate, 1% alkyl phenol polyoxyethylene ether, 15% solvent naphtha, 10% propylene glycol, add water to 100%.According to routine
It is 30% biguanides, three octyl benzene sulfonate prothioconazoles microemulsion that formulation method, which is configured to active constituent content,.
Embodiment 9
Weigh 30% biguanides, three octyl benzene sulfonate, 5% thiophene trifloxystrobin, 6% calcium dodecyl benzene sulfonate, 3% benzene second
Base phenol polyethenoxy ether phosphate, 2% agriculture breast 33#, 1% alkyl phenol polyoxyethylene ether, 10% isopropanol add water to 100%.It will
The method that the above raw material routinely prepares microemulsion, which is put into mixing kettle, to be mixed, and it is that 40% biguanides three is pungent that active constituent content, which is made,
Alkylbenzene sulfonate thiophene trifloxystrobin microemulsion.
Embodiment 10
The synergy of bactericidal composition is studied
With three octyl benzene sulfonate (component one) of biguanides and Xinjunan acetate, azophenlyene α -2 carboxylic acids, prothioconazoles or thiophene
Trifloxystrobin (component two) has carried out the synergy research of mutual compounding, and specific method is：It measures component one first and component two is single
The EC of compound (hereinafter referred to as medicament)50Value (drug is to concentration in effective inhibition of germ), according in combination two single medicaments
EC50The portfolio ratio of value setting medicament, and coefficient of synergism R values are calculated according to Wadley formula, determine suitable composition ratio
Example.
Experiment target pathogens be pear cucumerinum, Powdery Mildew, alternaria, brown patch germ, but not limited to this.Experiment
Method refers to《People's Republic of China (PRC) agricultural industry criteria NY/T1156.2-2006》, using disleave method and mycelial growth rate
Method is measured.
Respectively by pear cucumerinum, pears Powdery Mildew conidial suspension 1 × 105~106A/ml connects spraying kind in pears
Leaf on piece (now with the current), the reagent agent for different series concentration of spraying afterwards for 24 hours, respectively at 18 DEG C and 28 DEG C of constant incubators
Middle culture investigates disease index after 7 days.
Disease index=∑ (the disease numbers of sheets at different levels × opposite value of series)/(investigation total number of sheets × 9) × 100
State of an illness inhibiting rate (%)=(control disease index-processing disease index)/control disease index × 100
Pear black spot, brown patch germ stem are inoculated in PDA plate center, 25 DEG C are cultivated 3 days, are existed with sterile card punch
Bacteria cake (bacteria cake diameter Φ=5mm) is made in punching at nearly colony edge.By the access of bacteria cake face down containing series concentration for examination
The PDA culture medium tablet center of medicament, each culture dish are inoculated with bacteria cake 1, are repeated 4 times per concentration for the treatment of.It is trained in 25 DEG C of constant temperature
It supports after being cultivated 3 days in case, colony growth diameter is measured using crossing method, its average value is taken, is calculated according to following equation
Average production inhibiting rate (%).The data obtained through Finney probit analysis methods, with DPS statistical softwares find out virulence regression equation,
EC50Value and related coefficient, as a result as shown in table 1 to table 16.
Mycelial growth inhibition rate (%)=(control colony diameter-processing colony diameter)/control colony diameter × 100
Coefficient of synergism (SR) is calculated according to Wadley methods.The joint poison of compounding agent is evaluated by comparing coefficient of synergism (SR)
Force effect.
EC50(theory)=(a+b)/(a/EC50A+b/EC50B)
SR=EC50(theory)/EC50(reality)
A, B respectively represents two kinds of dosage components；A, b is the ratio of two component of A, B quality in compounded combination.
It is antagonism when SR≤0.5；It is summation action when SR=0.5~1.5；It is synergistic effect when SR >=1.5.
1 biguanides of table, three octyl benzene sulfonate and Xinjunan acetate composition measure the Wadley methods of pear cucumerinum
As a result
2 biguanides of table, three octyl benzene sulfonate and Xinjunan acetate composition measure the Wadley methods of pears Powdery Mildew
As a result
3 biguanides of table, three octyl benzene sulfonate and Xinjunan acetate composition measure the Wadley methods of Pear black spot bacterium
As a result
4 biguanides of table, three octyl benzene sulfonate and Xinjunan acetate composition measure the Wadley methods of pears brown patch germ
As a result
5 biguanides of table, three octyl benzene sulfonate and azophenlyene α -2 carboxylic acid compositions measure the Wadley methods of pear cucumerinum
As a result
6 biguanides of table, three octyl benzene sulfonate and azophenlyene α -2 carboxylic acid compositions measure the Wadley methods of pears Powdery Mildew
As a result
7 biguanides of table, three octyl benzene sulfonate and azophenlyene α -2 carboxylic acid compositions measure the Wadley methods of Pear black spot bacterium
As a result
8 biguanides of table, three octyl benzene sulfonate and azophenlyene α -2 carboxylic acid compositions measure the Wadley methods of pears brown patch germ
As a result
The Wadley method measurement results of 9 biguanides of table, three octyl benzene sulfonate and prothioconazoles composition to pear cucumerinum
10 biguanides of table, three octyl benzene sulfonate and prothioconazoles composition measure knot to the Wadley methods of pears Powdery Mildew
Fruit
11 biguanides of table, three octyl benzene sulfonate and prothioconazoles composition measure knot to the Wadley methods of Pear black spot bacterium
Fruit
12 biguanides of table, three octyl benzene sulfonate and prothioconazoles composition measure knot to the Wadley methods of pears brown patch germ
Fruit
13 biguanides of table, three octyl benzene sulfonate and thiophene trifloxystrobin composition measure knot to the Wadley methods of pear cucumerinum
Fruit
14 biguanides of table, three octyl benzene sulfonate and thiophene trifloxystrobin composition measure knot to the Wadley methods of pears Powdery Mildew
Fruit
15 biguanides of table, three octyl benzene sulfonate and thiophene trifloxystrobin composition measure knot to the Wadley methods of Pear black spot bacterium
Fruit
16 biguanides of table, three octyl benzene sulfonate and thiophene trifloxystrobin composition measure knot to the Wadley methods of pears brown patch germ
Fruit
Measurement result shows:Three octyl benzene sulfonate (component one) of biguanides and Xinjunan acetate, azophenlyene α -2 carboxylic acids,
Prothioconazoles or thiophene trifloxystrobin (component two) are with weight ratio for 1:10~10:1 is mixed, to pear cucumerinum, Powdery Mildew, blackspot
Germ, brown patch germ have higher virulence, the two mixture to have significant synergies.
Medicament used in following embodiment can refer to embodiment 1-9 and prepare.
Embodiment 11
Bactericidal composition tests the control effect of pear scab
According to medicine inspecting institute of the Ministry of Agriculture《Pesticide field efficacy medicine test criterion》" bactericidal agent for preventing and treating pears are black by GBT17980.24-2000
Star disease field control effectiveness test criterion " is tested.
Experimental plot is located at the villages Bei little Chen of Xinji City of Hebei province, 10 days spray first time medicines, mu liquid volume after pear tree thanks and spends
250KG, continuous use 4 times, medication interval 10-15 days, 14 days investigation field incidences, calculate disease index after last medication
And control effect.
Drug effect computational methods：
Disease index=∑ (the disease numbers of sheets at different levels × opposite the value of series)/control effect (%) of (investigation total number of sheets × 9) × 100
=(blank district disease index-prevention area disease index)/blank district disease index × 100
17 biguanides of table, three octyl benzene sulfonate (component one) and Xinjunan acetate, azophenlyene α -2 carboxylic acids, prothioconazoles or
Thiophene trifloxystrobin (component two) bactericidal composition prevents the field efficacy of pear scab
Note：Letter in " significance of difference " column represents the new multipole significant difference of Duncan and carries out analysis result.
The result shows that containing three octyl benzene sulfonate (component one) of biguanides and Xinjunan acetate, azophenlyene α -2 carboxylic acids,
Prothioconazoles or the bactericidal composition of thiophene trifloxystrobin (component two) have good control effect to pear scab, in same dose
Or higher control effect can be reached under relatively low dosage, and the preventive effect managed everywhere in the bactericidal composition under dosage is above
85%, it is significantly higher than comparison medicament.
Embodiment 12
Bactericidal composition tests the control effect of pears powdery mildew
According to medicine inspecting institute of the Ministry of Agriculture《Pesticide field efficacy medicine test criterion》GB/T17980.10-2000 " bactericidal agent for preventing and treating pears
Powdery mildew field control effectiveness test criterion " is tested
Experimental plot is located at the villages Hebei province Botou City great Hu village, starts to spray first time medicine, mu medication when pears powdery mildew occurs
Liquid measure 250KG, continuous use 3 times, medication interval 30 days, 20 days investigation field incidences after last medication calculate the state of an illness and refer to
Number and control effect.
Drug effect computational methods：
Disease index=∑ (the disease numbers of sheets at different levels × opposite value of series)/(investigation total number of sheets × 9) × 100
Control effect (%)=(blank district disease index-prevention area disease index)/blank district disease index
×100
18 biguanides of table, three octyl benzene sulfonate (component one) and Xinjunan acetate, azophenlyene α -2 carboxylic acids, prothioconazoles or
Thiophene trifloxystrobin (component two) bactericidal composition prevents the field efficacy of pears powdery mildew
Note：Letter in " significance of difference " column represents the new multipole significant difference of Duncan and carries out analysis result.
The result shows that containing three octyl benzene sulfonate (component one) of biguanides and Xinjunan acetate, azophenlyene α -2 carboxylic acids,
Prothioconazoles or the bactericidal composition of thiophene trifloxystrobin (component two) have good control effect to pears powdery mildew, in same dose
Or higher control effect can be reached under relatively low dosage, and the preventive effect managed everywhere in the bactericidal composition under dosage is above
85%, it is significantly higher than comparison medicament.
Embodiment 13
Bactericidal composition tests the control effect of Pear black spot
According to medicine inspecting institute of the Ministry of Agriculture《Pesticide field efficacy medicine test criterion》" bactericidal agent for preventing and treating pears are black by GB/T1798044-2000
Pinta field control effectiveness test criterion " is tested.
Experimental plot is located at Hebei province Zhao County Xie village, starts to use first time medicine, mu liquid when Pear black spot is sporadicly fallen ill
250KG, continuous use 3 times are measured, medication interval 10-15 days investigates field incidence in 14 days after last medication, calculates the state of an illness and refer to
Number and control effect.
Drug effect computational methods：
Disease index=∑ (the disease numbers of sheets at different levels × opposite value of series)/(investigation total number of sheets × 9) × 100
Control effect (%)=(blank district disease index-prevention area disease index)/blank district disease index
×100
19 biguanides of table, three octyl benzene sulfonate (component one) and Xinjunan acetate, azophenlyene α -2 carboxylic acids, prothioconazoles or
Thiophene trifloxystrobin (component two) bactericidal composition prevents the field efficacy of Pear black spot
Note：Letter in " significance of difference " column represents the new multipole significant difference of Duncan and carries out analysis result.
The result shows that containing three octyl benzene sulfonate (component one) of biguanides and Xinjunan acetate, azophenlyene α -2 carboxylic acids,
Prothioconazoles or the bactericidal composition of thiophene trifloxystrobin (component two) have good control effect, each treatment dosage to Pear black spot
Under preventive effect be above 82%, be significantly higher than the preventive effect of comparison medicament.
Embodiment 14
Bactericidal composition tests the control effect of pears brown spot
According to medicine inspecting institute of the Ministry of Agriculture《Pesticide field efficacy medicine test criterion》GB/T17980.10-2000 " bactericidal agent for preventing and treating pears
Brown spot field control effectiveness test criterion " is tested
Experimental plot is located at the villages Hebei province Botou City great Hu village, starts to spray first time medicine, mu when pears brown spot sporadicly occurs
With liquid volume 250KG, continuous use 3 times, medication interval 14-25 days, according to the state of an illness and weather conditional decision administration time, last
14 days investigation field incidences, calculate disease index and control effect after medication.
Drug effect computational methods：
Disease index=∑ (the disease numbers of sheets at different levels × opposite value of series)/(investigation total number of sheets × 9) × 100
Control effect (%)=(blank district disease index-prevention area disease index)/blank district disease index
×100
20 biguanides of table, three octyl benzene sulfonate (component one) and Xinjunan acetate, azophenlyene α -2 carboxylic acids, prothioconazoles or
Thiophene trifloxystrobin (component two) bactericidal composition prevents the field efficacy of pears brown spot
Note：Letter in " significance of difference " column represents the new multipole significant difference of Duncan and carries out analysis result.
The result shows that containing three octyl benzene sulfonate (component one) of biguanides and Xinjunan acetate, azophenlyene α -2 carboxylic acids,
Prothioconazoles or the bactericidal composition of thiophene trifloxystrobin (component two) have good control effect to pears brown spot, in same dose
Or higher control effect can be reached under relatively low dosage, and the preventive effect managed everywhere in the bactericidal composition under dosage is above
85%, it is significantly higher than comparison medicament.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (10)
1. a kind of bactericidal composition, which is characterized in that active constituent includes：Component one and component two；The component one is biguanides
Three octyl benzene sulfonates；The component two is appointing in Xinjunan acetate, azophenlyene α -2 carboxylic acids, prothioconazoles, thiophene trifloxystrobin
It is one or more of.
2. bactericidal composition according to claim 1, which is characterized in that the mass ratio of component one and component two is 1:20~
20:1, preferably 1:15~15:1, further preferably 1:10~10:1, more preferably 1:8~8:1, still more preferably for
1:5~5:1, preferably 1:1.
3. bactericidal composition according to claim 1 or 2, which is characterized in that active constituent is by two groups of component one and component
At.
4. a kind of fungicide, separately made by any one of the claim 1-3 bactericidal compositions, or is appointed by claim 1-3
One bactericidal composition is made with the available auxiliary agent of pesticide field or carrier.
5. fungicide according to claim 4, which is characterized in that it is water dispersible granules, aqueous emulsion or microemulsion.
6. fungicide according to claim 4 or 5, which is characterized in that the gross mass of the component one and component two, which accounts for, kills
The 5%~80% of microbial inoculum gross mass, preferably 20%~60%.
7. fungicide according to claim 4, which is characterized in that it is water dispersible granules, including 6-35% biguanides three is pungent
Component two, 1-5% alkyl phenol polyoxyethylene ether, 1-3% Negels, 5- described in alkylbenzene sulfonate, 1.8-54%
10% lignosulfonates, 1-3% sodium lignin sulfonates, 1-3% lauryl sodium sulfate, 1-5% neopelexes,
5-10%NN0,10-15% urea, 1-3% magnesium chlorides, 1-5% precipitated calcium carbonates, 1-3% starch, cornstarch or ammonium sulfate
Complement to 100%.
8. fungicide according to claim 4, which is characterized in that it is aqueous emulsion, including 5-10% thiophenes trifloxystrobin, 1-
15% component two, 1-5% phenethyl phenol polyethenoxy ethers phosphate, 1-3% alkyl phenol polyoxyethylene ether, 1-3% agriculture breasts
33#, 10-15% solvent naphtha, Jia Shui complement to 100%.
9. fungicide according to claim 4, which is characterized in that it is microemulsion, including 5-30% thiophenes trifloxystrobin, 5-
20% component two, 1-10% calcium dodecyl benzene sulfonates, 1-5% neopelexes, 1-5% phenethyl phenol polyoxies
Ethylene ether phosphate, 1-3% alkyl phenol polyoxyethylene ether, 1-3% agriculture breasts 33#, 10-15% solvent naphtha, 10% propylene glycol or
10% isopropanol, Jia Shui complement to 100%.
10. any one of any one of the claim 1-3 bactericidal compositions or claim 4-9 fungicide is in crop disease
Purposes in evil prevention；
Preferably, the disease includes scab, powdery mildew, black spot, brown spot；
With or, the crops include pears apple, grape, peach, plum, apricot, cherry, citrus.
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| WO2020232779A1 (en) * | 2019-05-22 | 2020-11-26 | 安徽久易农业股份有限公司 | Prothioconazole dry suspending agent, preparation method therefor and application thereof |
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| CN103039493A (en) * | 2012-10-11 | 2013-04-17 | 江苏龙灯化学有限公司 | Bactericidal composition containing active ingredients bellkute and triflumizole and application of bactericidal composition |
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| CN103988845B (en) * | 2014-05-29 | 2015-09-09 | 江苏龙灯化学有限公司 | A kind of Fungicidai mixtures |
| CN105010380B (en) * | 2015-07-08 | 2018-06-22 | 广东中迅农科股份有限公司 | Bactericidal composition containing three octyl benzene sulfonate of biguanides and chitosan |
| CN106417346B (en) * | 2016-09-20 | 2019-02-05 | 佛山市盈辉作物科学有限公司 | A kind of bactericidal composition containing three octyl benzene sulfonate and fluopyram of biguanides |
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| CN103039493A (en) * | 2012-10-11 | 2013-04-17 | 江苏龙灯化学有限公司 | Bactericidal composition containing active ingredients bellkute and triflumizole and application of bactericidal composition |
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| 刘新等: "《农药安全使用技术》", 30 September 2014 * |
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| WO2020232779A1 (en) * | 2019-05-22 | 2020-11-26 | 安徽久易农业股份有限公司 | Prothioconazole dry suspending agent, preparation method therefor and application thereof |
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