CN102771488B - Bactericidal composition containing isothiazolone - Google Patents
Bactericidal composition containing isothiazolone Download PDFInfo
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- CN102771488B CN102771488B CN201210267852.8A CN201210267852A CN102771488B CN 102771488 B CN102771488 B CN 102771488B CN 201210267852 A CN201210267852 A CN 201210267852A CN 102771488 B CN102771488 B CN 102771488B
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Abstract
The invention discloses a bactericidal composition containing isothiazolone. The bactericidal composition containing the isothiazolone comprises effective active ingredients including a first active ingredient isothiazolone and a second active ingredient strobilurin fungicide, wherein the second active ingredient strobilurin fungicide is selected from azoxystrobin, kresoxim-methyl, pyraclostrobin, trifloxystrobin and orysastrobin, and the weight ratio of the first active ingredient to the second active ingredient is 1:100-5:1 and preferably is 1:50-1:1. The bactericidal composition can be prepared into microemulsion, suspension emulsion and paste which are allowable agriculturally. The bactericidal composition is reasonable in ingredients, good in bactericidal effect and low in pesticide cost, and the activity and the bactericidal effect are not produced by simple superposition of activity of the ingredients. Compared with existing single preparations, except the remarkable bactericidal effect, the bactericidal composition has remarkable synergistic effect, is free of pollution to crops and good in safety, meets the safety requirements for pesticide preparations and has remarkable prevention and control effect on rotten diseases of apple trees, rice blast and banded sclerotial blight of rice.
Description
Technical field
The present invention relates to a kind of bactericidal composition of isothiazolone-containing, particularly a kind of isothiazolinone is as the first active component, be selected from the methoxy acrylic bactericide of Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, orysastrobin, SYP-3375 as the bactericidal composition of the second active component, belong to technical field of compounded pesticide.
Background technology
The mixture that isothiazolinone is made up of CMIT (CMI) and MIT (MI), technical indicator is carried out professional standard HG/T 3657-2008, trade name: CIT/MIT, KF88, Kathon CG, Kathon.Isothiazolinone is a kind of wide spectrum, efficient, low toxicity, non-oxidative bactericide, plays to kill livestock and acts on by disconnecting the key of bacterium and algae protein matter.After isothiazolinone contacts with microorganism, can promptly irreversibly suppress its growth, thereby cause the death of microbial cell, common bacteria, fungi, algae etc. are had to very strong inhibition and killing action.The efficiency of killing livestock is high, and degradability is good, has residual, handling safety, the feature that stability is strong of not producing.Isothiazolinone control corps diseases has no report, show that through Shanggezhilu Biosciences Co., Ltd., Shaanxi's field control effectiveness test isothiazolinone has good control efficiency to diseases such as paddy rice bacterial leaf spot, apple valsa cankers, but the isothiazolinone lasting period is shorter, cause medication number of times more.
Methoxy acrylic acid series bactericidal agent is in recent years bactericide kind newly developed, is a large milestone in bactericide field.Methoxy acrylic bactericide has unique mechanism of action, the active group of this series bactericidal agent is methoxy acrylic acid (ester/acid amides), the cytochrome b el compound of Main Function in the mitochondrial respiratory chain of fungi, thus the growth of electronics transmission Antifungi stoped.There is protection, treat, root out, infiltration, systemic activity, and can be in plant corpus, degraded very soon in soil and water.Although this series bactericidal agent mechanism of action uniqueness, the same with other bactericide, methoxy acrylic bactericide is also unable to escape the misfortune of resistance, and use cost is high, uses so limited the spread of methoxy acrylic acid series bactericidal agent.Administering resistance is first mixed, therefore, by composite bactericide combinations different from the another kind of mechanism of action methoxy acrylic acid series bactericidal agent, improves drug effect, slows down the generation of resistance, reduces usage amount, and expanding using of such medicament is current urgent problem.
Introduce methoxy acrylic acid series bactericidal agent principal item below:
Fluoxastrobin, other Chinese name: Amici reaches, English common name: azoxystrobin, chemical name: (E) 2-[2-[6-(2-cyano-benzene oxygen) pyrimidine-4-yl oxygen] phenyl]-3-methoxy-methyl acrylate.Efficiently, wide spectrum; almost can prevent and treat all fungal diseases; as powdery mildew, rust, glume blight, net blotch, downy mildew, rice blast etc. are all had to good activity, there is protection, root out, infiltration and systemic action, can suppress the sprouting of spore and the growth of mycelia.Can be used for cauline leaf processing, seed treatment, also can carry out soil treatment.Suitable crop is cereal, paddy rice, grape, potato, vegetables, fruit tree, beans and other crop etc.
Kresoxim-methyl, other Chinese name: emerald green shellfish, English common name: kresoxim-methyl, chemical name: methyl (E)-2-methoxyimino-2-[2-(O-toloxyl) phenyl] acetate.Kresoxim-methyl can suppress Spores and invade; there is good prolection; kresoxim-methyl has good activity to fungus-caused multiple diseases such as imperfect fungus, sac fungi, basidiomycetes, Oomycetes; as: the diseases such as grape powdery mildew, wheat rust, potato late blight, pumpkin epidemic disease, rice blast, especially to powdery mildew of strawberry, melon powdery mildew, powdery mildew of cucumber, pear scab special efficacy.
Pyraclostrobin, has another name called pyraclostrobin, English common name: Pyraclostrobin, chemical name: N-{2-[1-(4-chlorphenyl)-1H-pyrazole-3-yl oxygen methyl] phenyl } (N-methoxyl group) carbamate.Can prevent and kill off all types of fungal pathogens, fungicidal spectrum is wide, to various crop, comprises that the multiple diseases of wheat class, grape, fruit tree, vegetables is effective, is mainly used in preventing and treating grape powdery mildew, downy mildew; Wheat powdery mildew, rust; Leaf rust of barley; The black leaf cecospora spot of banana; Tomato morning, late blight etc.
Oxime bacterium ester, English common name: trifloxystrobin, chemical name: methyl (E) methoxyl group imido grpup-(E)-α-[1-(α, the fluoro-m-tolyl of α-tri-)-ethyleneimino oxygen base]-o-tolyl } ethyl acetate.The exploitation of Wo Junzhishi Beyer Co., Ltd; there is protection, treatment, infiltration and excellent conductive performance; resistance of rainwater washing against, is the blade face bactericide of a wide spectrum, and its high efficiency and good crop-selective make it can effectively prevent and treat the disease on temperate zone, subtropical crop.Can be used for control rust, downy mildew, apple scab, damping off, powdery mildew etc.Suitable crop is grape, apple, wheat, peanut, banana, vegetables, paddy rice etc.
Orysastrobin, English common name: orysastrobin, chemical name: (2E)-2-(methoxyimino)-2-{2-[(3E, 5E, 6E)-S-(methoxyimino)-4,6-dimethyl-2,8-dioxa-3, the 7-dioxa ninth of the ten Heavenly Stems-3,6-diene-1-yl] phenyl }-N-methylacetamide.Be the methoxy acrylic bactericide that BASF Aktiengesellschaft researched and developed in nineteen ninety-five, be mainly used at present paddy rice control rice blast and banded sclerotial blight.
SYP-3375, English common name: coumoxystrobin, chemical name: (E)-2-(2-((3-butyl-4-methyl-coumarin-7-Ji-oxygen base) methyl) phenyl)-3-methoxy-methyl acrylate.There is protection and therapeutic action; be a kind of disinfectant use in agriculture of high-efficiency broad spectrum, the plant disease causing by mastigomycetes, in conjunction with bacterium, sac fungi, basidiomycetes and imperfect fungus is had to good preventive effect as sclerotinia rot of colza, apple valsa canker, rice blast, sigatoka, alternaria leaf spot of apple etc.
Inventor finds by test, by composite to the isothiazolinone different mechanism of action and methoxy acrylic bactericide, has significant synergies; can significantly improve control efficiency, the minimizing dosage for the treatment of, protection; expanding prevention spectrum, reduces cost accounting, high to crop safety.
Summary of the invention
The object of the present invention is to provide a kind of component reasonable, synergistic effect is remarkable, and bactericidal effect is good, and drug cost is low, is difficult for developing immunity to drugs, bactericidal composition to crop safety.
Another object of the present invention is to provide above-mentioned composition in the application preventing and treating in apple valsa canker, rice blast, rice sheath blight disease.
In order to overcome the defect of existing unitary agent, technical scheme of the present invention is to solve like this:
A) the first active component is isothiazolinone;
B) the second active component is the methoxy acrylic bactericide that is selected from Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, orysastrobin, SYP-3375;
The weight ratio of the first active component and the second active component is 1:100～5:1, is preferably 1:50～1:1, and more preferably 1:25～1:1, most preferably is 1:10～1:5.
The cumulant of the first active component and the second active component is 0.1%～60% of described composition total weight, is preferably 0.1%～50%.
The agricultural bactericidal composition of a kind of isothiazolone-containing of the present invention, the preparation formulation that can prepare according to the known method of those skilled in the art of the present technique is microemulsion, suspending agent, paste.
To microemulsion, spendable auxiliary agent has: emulsifier is as calcium dodecyl benzene sulfonate (agriculture breast 500#), Nongru-700 #(common name: alkyl phenol formaldehyde resin polyoxyethylene ether), agriculture breast 2201#, this dish-60#(common name: Span60), Tween-60 #(common name: polyoxyethylene sorbitan stearate), TX-10(common name: octyl phenol polyoxy ethene (10) ether), agriculture breast 1601(common name: triphen ethyl-phenol polyoxyethylene polyoxypropylene block polymer), in agriculture breast 600#, the newborn 400# of agriculture one or more; Assistant for emulsifying agent as in methyl alcohol, isopropyl alcohol, n-butanol, ethanol one or more; Solvent as cyclohexanone, 1-METHYLPYRROLIDONE, dimethylbenzene, toluene, solvent naphtha (trade mark: S-150, S-180, S-200) one or more; Water is deionized water.
To suspending agent, spendable auxiliary agent has: dispersant is produced as polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate (dispersing agent NNO), Huntsman Corporation of the TERSPERSE 2020(U.S., alkyl naphthalene sulfonic acid salt) in one or more; Emulsifier is as Nongru-700 #(common name: alkyl phenol formaldehyde resin polyoxyethylene ether), agriculture breast 2201, this dish-60#(common name: Span60), Tween-60 #(common name: polyoxyethylene sorbitan stearate), agriculture breast 1601#(common name: triphen ethyl-phenol polyoxyethylene polyoxypropylene block polymer), one or more in TERSPERSE 4894; Wetting agent as in alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, TERSPERSE 2500 one or more; Thickener as in xanthans, polyvinyl alcohol, bentonite, aluminium-magnesium silicate one or more; Preservative as in formaldehyde, benzoic acid, Sodium Benzoate one or more; Defoamer is as organic silicon defoamer; Antifreezing agent as ethylene glycol, propane diols, glycerine, urea, inorganic salts as in sodium chloride one or more; Water is deionized water.
To paste, available auxiliary agent has: dispersant is as polycarboxylate, alkylnaphthalene sulfonate, one or more in lignosulfonates, alkylphenol-polyethenoxy base ether formaldehyde condensation products phosphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate; Film forming agent is as one or both mixture in polyacrylamide, acrylic resin, butadiene resin, polyurethane, cellulose nitrate film forming agent; Thickener is any one or two kinds of mixtures in carboxymethyl cellulose, aluminium-magnesium silicate, in bentonite; Antifreezing agent be ethylene glycol, propane diols any one; Water is deionized water.
Component of the present invention is reasonable, and treatment adds protective effect, and bactericidal effect is good, and drug cost is low, and its active and bactericidal effect is not the simple superposition of each composition activity, but has significant synergistic effect, good to crop safety, meets the security requirement of pesticidal preparations.The present invention has good control efficiency to apple valsa canker, rice blast, rice sheath blight disease.
Embodiment
In order to prevent and treat apple valsa canker, rice blast, the banded sclerotial blight in agricultural production, inventor has carried out mutual composite synergy research with isothiazolinone and methoxy acrylic bactericide Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, orysastrobin, SYP-3375, and concrete grammar is:
Subjects is respectively apple valsa canker (Cytospora mandshurica), Pyricularia oryzae (Pyriculariagrisea), Rhizoctonia solani Kuhn (Rhizoctonia solani), former medicine is mixed with to the test medicine needing, test method is with reference to " People's Republic of China's agricultural industry criteria NY/T1156.2-2006 ".First single dose and each Mixed Pharmacy are arranged to 5 variable concentrations gradients (on the basis of preliminary experiment result, different with proportioning according to different medicament combinations, concentration gradient setting is also different, and bacteriostasis rate is set by geometric progression in 5%～90% scope).If clear water contrast, repeats 3 times.Adopt mycelial growth rate method, by cultured pathogen, be 5 mm card punch with diameter under aseptic condition, cuts bacterium cake from colony edge, bacterium cake is connected on the culture medium flat plate of different drug concentrations with transfer needle, puts in 27 DEG C, saturated humidity incubator and cultivate; In the time that control group bacterium colony covers with, measure colony diameter, calculate the percentage that each chemicals treatment suppresses mycelial growth, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC of each medicament
50value, with the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun mixture, evaluates the activity of reagent agent to germ with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 < CTC < 120 show as summation action.
Toxicity Determination result shows: isothiazolinone has good virulence as 1:100～5:1 is mixed to apple canker, rice blast, rice sheath blight disease taking weight ratio with methoxy acrylic bactericide Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, orysastrobin, SYP-3375, all has significant synergistic effect.
Below in conjunction with embodiment, content of the present invention is described further.
Example of formulations 1
Take 3% isothiazolinone, 30% Fluoxastrobin, 4%TX-10,5% Nongru-700 #, 6% agriculture breast 500#, 4% agriculture breast 1601#, 15% cyclohexanone, 5%N-methyl pyrrolidone, 5% n-butanol, through dissolving completely and mixing, deionized water adds to 100% weight portion, makes 33% isothiazolinone Fluoxastrobin microemulsion after stirring.
Example of formulations 2
Take 2% isothiazolinone, 28% kresoxim-methyl, 4% Nongru-700 #, 3% agriculture breast 500#, 6% agriculture breast 600#, 10% dimethylbenzene, 5% cyclohexanone, 5% isopropyl alcohol, through dissolving completely and mixing, deionized water adds to 100% weight portion, makes 30% isothiazolinone kresoxim-methyl microemulsion after stirring.
Example of formulations 3
Take 0.5% isothiazolinone, 25% pyraclostrobin, 4%TX-10,5% Nongru-700 #, 6% agriculture breast 500#, 4% agriculture breast 1601#, 15% cyclohexanone, 5%N-methyl pyrrolidone, 5% n-butanol, through dissolving completely and mixing, deionized water adds to 100% weight portion, makes 25.5% isothiazolinone pyraclostrobin microemulsion after stirring.
Example of formulations 4
Take 2% isothiazolinone, 16% oxime bacterium ester, 5% Nongru-700 #, 3% agriculture breast 500#, 6% agriculture breast 600#, 10% dimethylbenzene, 13% cyclohexanone, 5% ethanol, through dissolving completely and mixing, deionized water adds to 100% weight portion, makes 18% isothiazoline ketoxime bacterium ester microemulsion after stirring.
Example of formulations 5
Take 3% isothiazolinone, 12% orysastrobin, 5% Nongru-700 #, 3% agriculture breast 500#, 6% agriculture breast 600#, 5% dimethylbenzene, 20% cyclohexanone, 5% ethanol, through dissolving completely and mixing, deionized water adds to 100% weight portion, makes 15% isothiazolinone orysastrobin microemulsion after stirring.
Example of formulations 6
Take 2% isothiazolinone, 22% SYP-3375,5% Nongru-700 #, 3% agriculture breast 500#, 6% agriculture breast 600#, 10% dimethylbenzene, 20% cyclohexanone, 5% ethanol, through dissolving completely and mixing, deionized water adds to 100% weight portion, makes 24% isothiazolinone SYP-3375 microemulsion after stirring.
Example of formulations 7
Take 0.5% isothiazolinone, 50% Fluoxastrobin, 4% Huntsman Corporation of the TERSPERSE 4894(U.S. produces), 1.5% Huntsman Corporation of the TERSPERSE 2500(U.S. produces), 2% Huntsman Corporation of the TERSPERSE 2020(U.S. produces), 1% aluminium-magnesium silicate, 0.2% xanthans, 5% ethylene glycol, 0.5% formaldehyde, 0.5% silicone defoaming agent (trade name: s-29 Nanjing "four news" (new ideas applied chemistry product company produces), deionized water add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 50.5% isothiazolinone Fluoxastrobin suspending agent after sand mill sand milling.
Example of formulations 8
Take 10% isothiazolinone, 50% kresoxim-methyl, 5%TERSPERSE 4894,2%TERSPERSE 2500,3%TERSPERSE 2020,0.2% xanthans, 5% propane diols, 0.5% benzoic acid, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 60% isothiazolinone kresoxim-methyl suspending agent after sand mill sand milling.
Example of formulations 9
Take 5% isothiazolinone, 25% pyraclostrobin, 2%TERSPERSE 2020,2% this dish-60#, 2% Tween-60 #, 1%TERSPERSE 2500,0.5% white carbon, 0.3% xanthans, 5% ethylene glycol, 0.5% benzoic acid, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 30% isothiazolinone pyraclostrobin suspending agent after sand mill sand milling.
Example of formulations 10
Take 5% isothiazolinone, 45% oxime bacterium ester, 1%TERSPERSE 2020,3% agriculture breast 1601#, 2% agriculture breast 2201#, 1%TERSPERSE 2500,0.3% xanthans, 5% ethylene glycol, 0.5% formaldehyde, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.High speed shear is disperseed 30min, with making 50% isothiazoline ketoxime bacterium ester suspending agent after sand mill sand milling.
Example of formulations 11
Take 10% isothiazolinone, 5% orysastrobin, 4%TERSPERSE 4894,1.5%TERSPERSE 2500,1%TERSPERSE 2020,0.2% aluminium-magnesium silicate, 5% ethylene glycol, 0.5% formaldehyde, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 15% isothiazolinone orysastrobin suspending agent after sand mill sand milling.
Example of formulations 12
Take 1.5% isothiazolinone, 30% SYP-3375,3%TERSPERSE 4894,1.5%TERSPERSE 2500,1%TERSPERSE 2020,0.2% xanthans, 5% propane diols, 0.5% benzoic acid, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 31.5% isothiazolinone SYP-3375 suspending agent after sand mill sand milling.
Example of formulations 13
Take 0.05% isothiazolinone, 0.1% Fluoxastrobin, 3% lignosulfonates, 8% polyacrylamide, 5% aluminium-magnesium silicate, 2% ethylene glycol, deionized water and add to 100% weight portion.Above-mentioned raw materials through mixing, with adding film forming agent and thickener to be uniformly mixed after sand mill sand milling, makes 0.15% isothiazolinone Fluoxastrobin paste except film forming agent, thickener.
Example of formulations 14
Take 0.2% isothiazolinone, 0.04% kresoxim-methyl, 4% alkylnaphthalene sulfonate, 7% butadiene resin, 5% bentonite, 2% propane diols, deionized water and add to 100% weight portion.Above-mentioned raw materials through mixing, with adding film forming agent and thickener to be uniformly mixed after sand mill sand milling, makes 0.24% isothiazolinone kresoxim-methyl paste except film forming agent, thickener.
Example of formulations 15
Take 0.05% isothiazolinone, 0.05% SYP-3375,3% alkylphenol-polyethenoxy base ether phosphate, 3% acrylic resin, 28% bentonite, 2% ethylene glycol, deionized water and add to 100% weight portion.Above-mentioned raw materials through mixing, with adding film forming agent and thickener to be uniformly mixed after sand mill sand milling, makes 0.1% isothiazolinone SYP-3375 paste except film forming agent, thickener.
Biological Examples 1: prevent and treat apple valsa canker field trial
Within 2011, carried out example of formulations 13(0.15% isothiazolinone Fluoxastrobin paste in Baoji, Shaanxi province city), example of formulations 14(0.24% isothiazolinone kresoxim-methyl paste), example of formulations 15(0.1% isothiazolinone SYP-3375 paste) prevent and treat apple valsa canker field trial, verified the control efficiency of this medicament to apple valsa canker and the safety to apple.Test method is with reference to " pesticide field efficacy medicine test criterion (two) bactericidal agent for preventing and treating apple and Fungus of Pear Canker Disease scar recurrence test of pesticide effectiveness GB/T 17980. 116-2004 ".Trial crops is apple tree, and controlling object is apple valsa canker (Cytosporama ndshurica).Test is located at Baoji Changwu County, and soil is clay, and physical features is smooth, and soil fertility is even, pH value 7.1, fertility is better, experimental field cultivation condition uniformity, apple variety is Fuji apple, the age of tree is 14 years, and distance between rows and hills is 5m × 6m, and the generation over the years of this garden apple canker is heavier.Test medicine and dosage refer to table 4.Separately establish blank, every community becomes age tree to be no less than 5 strains, new sick scar more than 8 then, and Gong32Ge community, random district group is arranged.
Test spring apple canker occurance peak carry out, adopt the method for scraping the spot brushing medicament of curing the disease, except to sick scar (spot) brushing liquid, to it, around the good bark within the scope of 2cm also will abundant brushing liquid.
Investigation and statistical method:
Investigate drug effect late fall, investigate the total scab number in each community, recurrence scab number, calculate preventive effect.Observe the injury to the good bark in sick scar (spot) edge simultaneously.
Scab recurrence rate=(scab recurrence number/scab sum) × 100
Control efficiency (%)=(contrast scab recurrence rate-processing scab recurrence rate)/contrast scab recurrence rate × 100.
Field test results shows: 0.15% isothiazolinone Fluoxastrobin paste, 0.24% isothiazolinone kresoxim-methyl paste, 0.1% isothiazolinone SYP-3375 paste have significant control efficiency to apple valsa canker, preventive effect is respectively 94.1%, 91.8% and 97.5%, the preventive effect that is obviously better than each single dose, synergistic effect is obvious.
To the security survey of apple tree, each test is processed apple tree is occurred without poisoning phenomenon.
Result of the test shows, after isothiazolinone and Fluoxastrobin, kresoxim-methyl, SYP-3375 mixture, obviously to have improved the control efficiency to apple valsa canker, to apple development safety, is the desirable medicament of preventing and treating apple canker.
Biological Examples 3: control rice blast field trial
Within 2011, carry out example of formulations 1(33% isothiazolinone Fluoxastrobin microemulsion at Shaanxi Province's Hanzhong City), example of formulations 2(30% isothiazolinone kresoxim-methyl microemulsion), example of formulations 9(30% isothiazolinone pyraclostrobin suspending agent), example of formulations 4(18% isothiazoline ketoxime bacterium ester microemulsion), example of formulations 5(15% isothiazolinone orysastrobin microemulsion), example of formulations 6(24% isothiazolinone SYP-3375 microemulsion) control rice blast field trial, verify the control efficiency of these several medicaments to rice blast and the safety to paddy rice.Trial crops is paddy rice, and controlling object is rice blast (Pyricularia oryzae Cav.).Test is located at Hanzhong City Yang County, and test medicine and dosage refer to table 5.Separately establish contrast medicament and blank, every processing repeats for 4 times, every community area 30 ㎡, and Gong56Ge community, random district group is arranged.Adopt conventional spray-on process, mu dispenser liquid 50kg, evenly sprays paddy rice complete stool.Altogether dispenser 2 times, paddy rice booting latter stage and the each dispenser of full heading time 1 time.
Investigation and statistical method:
Investigation in 14 days after the 2nd dispenser, the sampling of 5 of investigation method Wei Mei communities, every some investigation 50 fringes, grade scale is as follows:
0 grade: anosis;
1 grade: every fringe loss is (morbidity of indivedual branch stalk) below 5%;
3 grades: every fringe loss 6%-20% (1/3 left and right branch stalk morbidity);
5 grades: every fringe loss 21%-50% (fringe neck or main shaft morbidity, grain half is flat);
7 grades: every fringe loss 51%-70% (morbidity of fringe neck, most of shrivelled kernel);
9 grades: every fringe loss 71%-100% (morbidity of fringe neck, causes dead ears).
Refer to calculate preventive effect according to disease.After each spray medicine after the 1st day and medicine some days, observe medicament and respectively process the impact on paddy rice.
Disease index=[Σ (sick spike number × relative level numerical value at different levels)/investigate total spike number × 9] × 100%
Control efficiency (%)=[(contrast disease index-processing disease index)/contrast disease index] × 100.
Field test results shows: 33% isothiazolinone Fluoxastrobin microemulsion, 30% isothiazolinone kresoxim-methyl microemulsion, 30% isothiazolinone pyraclostrobin suspending agent, 18% isothiazoline ketoxime bacterium ester microemulsion, 15% isothiazolinone orysastrobin microemulsion, every 667 ㎡ of 24% isothiazolinone SYP-3375 microemulsion press respectively 20g, 20g, 15g, 30g, 25g, 15g consumption, after medicine, the preventive effect of rice blast had been respectively to 89.96% in 14 days for the second time, 86.93%, 89.96%, 88.94%, 90.45%, 85.62%, composite rear synergy is remarkable, obviously be better than the preventive effect of each single dose to rice blast, difference reaches utmost point significance level (table 5).
To the security survey of paddy rice, within after spray medicine after the 1st day and medicine some days, to observe, each test is processed paddy rice is occurred without poisoning phenomenon.
Result of the test shows after isothiazolinone and Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, orysastrobin, SYP-3375 mixture, obviously improve the control efficiency to rice blast, to paddy growth safety, it is the desirable medicament of control rice blast.
Biological Examples 3: prevent and treat rice sheath blight disease field trial
Within 2011, carry out example of formulations 1(33% isothiazolinone Fluoxastrobin microemulsion in Xixiang County, Shaanxi Province), example of formulations 2(30% isothiazolinone kresoxim-methyl microemulsion), example of formulations 3(25.5% isothiazolinone pyraclostrobin microemulsion), example of formulations 10(50% isothiazoline ketoxime bacterium ester suspending agent), example of formulations 5(15% isothiazolinone orysastrobin microemulsion), example of formulations 6(24% isothiazolinone SYP-3375 microemulsion) prevent and treat rice sheath blight disease field trial.Test method is with reference to " GB/T 17980.20-2000 pesticide field efficacy medicine test criterion () bactericidal agent for preventing and treating rice sheath blight disease ".Trial crops is paddy rice, and controlling object is that (Rhizoctoniasolan i) for rice sheath blight disease.Before the test of rice field, do not use the bactericide of preventing and treating banded sclerotial blight, the equilibrium of paddy rice growing way, field rich water quality management is consistent.Altogether dispenser 2 times, the 1st time in booting stage, medicine is sprayed at interval for 14 days again the 2nd time, with amount of liquid medicine be 60kg/667 ㎡, employing manual sprayer evenly sprays.Test medicine and dosage refer to table 6.Separately establish blank, every processing repeats for 4 times, every community area 25 ㎡, and Gong56Ge community, random district group is arranged.
Investigation and statistical method, carry out efficacy survey for 14 days after the 2nd dispenser.5 samplings of every community diagonal, every is connected 5 clumps, totally 25 clumps, records total strain number, diseased plant number and sick progression.According to rice leaf sheath and blade Harm level classification, taking strain as unit.Duration of test is irregularly observed each treatment region rice leaf growth, heading flowering, Grain Filling situation.
0 grade: complete stool is anosis;
1 grade: quaterfoil and following leaf sheath thereof, blade morbidity (taking sword-like leave as first leaf);
3 grades: the 3rd blade and following leaf sheath thereof, blade morbidity;
5 grades: the second blade and following leaf sheath thereof, blade morbidity;
7 grades: sword-like leave blade and following leaf sheath thereof, blade morbidity;
9 grades: complete stool morbidity, withered ahead of time.
Calculate disease index and control efficiency according to recording incidence.
Diseased plant rate (%)=(morbidity strain number/investigate total strain number) × 100
Disease index=[∑ (diseased plant number × relative level numerical value at different levels)/(investigating total strain number × 9)] × 100
Control efficiency (%)=[(contrast disease index-processing disease index)/contrast disease index] × 100.
Field test results shows, 33% isothiazolinone Fluoxastrobin microemulsion, 30% isothiazolinone kresoxim-methyl microemulsion, 25.5% isothiazolinone pyraclostrobin microemulsion, 50% isothiazoline ketoxime bacterium ester suspending agent, 15% isothiazolinone orysastrobin microemulsion, 24% isothiazolinone SYP-3375 microemulsion has good control efficiency to rice sheath blight disease, every 667 ㎡ press respectively 20g, 20g, 40, 15, 25g, 15g consumption, to rice sheath blight disease for the second time after medicine the preventive effect of 14 days be respectively 90.22%, 88.95, 91.64%, 87.30%, 91.84%, 85.46%, obviously be better than 3% isothiazolinone aqua 50g/667 ㎡, 25% Fluoxastrobin suspending agent 50g/667 ㎡, 25% kresoxim-methyl suspending agent 50g/667 ㎡, 25% pyraclostrobin missible oil 50g/667 ㎡, 25% oxime bacterium ester suspending agent 50g/667 ㎡, 20% orysastrobin suspending agent 50g/667 ㎡, the preventive effect (table 6) of 20% SYP-3375 suspending agent 50g/667 ㎡ to rice sheath blight disease.
To the security survey of paddy rice, according to irregularly observing after medicine, the birth indexes such as each medication district rice leaf growth, heading flowering, Grain Filling and blank district do not have notable difference, without symptom of chemical damage.
Result of the test shows after isothiazolinone and Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, orysastrobin, SYP-3375 mixture, obviously improve the control efficiency to rice sheath blight disease, dosage and drug cost are reduced, to paddy growth safety, it is the desirable medicament of preventing and treating rice sheath blight disease.
In sum, composition of the present invention is to adopt two kinds of active component compound schemes, its activity and bactericidal effect are not the simple superposition of each composition activity, compared with existing unitary agent, except having significant bactericidal effect, and there is significant synergistic effect, pollution-free to crop, safety is good, meets the security requirement of pesticidal preparations.
Claims (8)
1. a bactericidal composition for isothiazolone-containing, is characterized in that, its active component is:
A) the first active component is isothiazolinone;
B) the second active component is the methoxy acrylic bactericide that is selected from Fluoxastrobin, kresoxim-methyl, pyraclostrobin, oxime bacterium ester, orysastrobin, SYP-3375;
The weight ratio of the first active component and the second active component is 1:100～5:1.
2. the bactericidal composition of isothiazolone-containing according to claim 1, is characterized in that: the weight ratio of the first active component and the second active component is 1:55～2:1.
3. the bactericidal composition of isothiazolone-containing according to claim 2, is characterized in that: the weight ratio of the first active component and the second active component is 1:25～1:1.
4. the bactericidal composition of isothiazolone-containing according to claim 3, is characterized in that: the weight ratio of the first active component and the second active component is 1:10～1:5.
5. the bactericidal composition of isothiazolone-containing according to claim 1, is characterized in that, the cumulant of the first active component and the second active component is described composition total weight 0.1%～60%.
6. the bactericidal composition of isothiazolone-containing according to claim 5, is characterized in that, the cumulant of the first active component and the second active component is described composition total weight 0.1%～50%.
7. according to the bactericidal composition of one of any described isothiazolone-containing of claim 1 to 4, it is characterized in that described combination dosage form is microemulsion, suspending agent, paste.
Described in claim 1 bactericidal composition of isothiazolone-containing in the application preventing and treating in apple valsa canker, rice blast, rice sheath blight disease.
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| CN101530099A (en) * | 2009-04-27 | 2009-09-16 | 陕西上格之路生物科学有限公司 | Isothiazolone-containing bactericidal composition |
| CN101796952A (en) * | 2009-02-06 | 2010-08-11 | 上海科王新型材料研究中心 | Isothiazolinone disinfecting agent |
| CN101990398A (en) * | 2008-04-07 | 2011-03-23 | 拜耳作物科学有限公司 | Stable aqueous spore-containing formulation |
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| CN101990398A (en) * | 2008-04-07 | 2011-03-23 | 拜耳作物科学有限公司 | Stable aqueous spore-containing formulation |
| CN101796952A (en) * | 2009-02-06 | 2010-08-11 | 上海科王新型材料研究中心 | Isothiazolinone disinfecting agent |
| CN101530099A (en) * | 2009-04-27 | 2009-09-16 | 陕西上格之路生物科学有限公司 | Isothiazolone-containing bactericidal composition |
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