CN104782641A - Zhongshengmycin-containing biological sterilization composition and application thereof - Google Patents
Zhongshengmycin-containing biological sterilization composition and application thereof Download PDFInfo
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Abstract
The invention discloses a Zhongshengmycin-containing biological sterilization composition. The active ingredients are Zhongshengmycin and phenazino-1-carboxylic acid, wherein the weight ratio of Zhongshengmycin to phenazino-1-carboxylic acid is 1: (1-35); the Zhongshengmycin-containing biological sterilization composition further can contain auxiliary materials allowed and acceptable in pesticide, and can be wettable powder, suspensions, missible oil, water emulsions, water dispersible granules or micro-emulsion in formulation. According to the Zhongshengmycin-containing biological sterilization composition, the active ingredients Zhongshengmycin and phenazino-1-carboxylic acid are completely different in action mechanism, and can be compounded to realize multi-site action on pathogen and synergism, so as to expand the bactericidal spectrum, improve the control effect, reduce the dosage of pesticides, delay the generation of drug resistance, and reduce the risk of resistance. The Zhongshengmycin-containing biological sterilization composition is used for controlling a variety of diseases of fruit trees and vegetables, is especially suitable for controlling fruit tree crown gall, ginger wilt, root rot and other soil-borne fungal and bacterial diseases, meanwhile, can control rice sheath blight, grape downy mildew, apple altermaria leaf spot and other diseases of crops and fruit trees.
Description
Technical field
The present invention relates to a kind of biological antibiotic composition containing Zhongshengmycin and application thereof, in biopesticide Prevention Technique field.
Background technology
At present, chemical control is the important measures controlling day by day serious plant disease, advantages such as there is instant effect, fungicidal spectrum is wide, cost is low, be easy to use, important function has been played to guarantee agricultural production, but long-term a large amount of use of chemical bactericide causes a series of serious ecological problem and the social concern such as agricultural chemicals pesticide resistance, residue of pesticide, environmental pollution, agricultural product security.In recent years, along with the quality safety of people to agricultural product and the concern of problem of environmental pollution, the use of chemical bactericide is subject to more and more stricter restriction.Therefore, people start more to be conceived to more low toxicity, environmental protection, free of contamination biogenic pesticide.Biogenic pesticide utilizes the antagonist properties between microorganism to prevent and treat pathogen, and this is more efficient and environmental protection than existing chemical pesticide.At present, exploitation biogenic pesticide new varieties and complex preparation are more and more subject to people's attention.
Zhongshengmycin is a kind of novel agricultural antibiotic, and its fungicidal spectrum is wide, all has good preventive effect to the bacterial disease of crops and fungal disease, can the synthesis of anti-bacteria bacterium protein, causes thalline dead; There is strong inhibitory action to fungus spore germination, cause protoplasm cohesion in hyphal cell, change the form of thread mycelia.
Shen piperazine mycin is a kind of antibiotic secreted through biological culture by pseudomonas fluorescens, main component is phenazine-1-carboxylic acid (phenazine-1-carboxylic acid), there is wide spectrum Suppressing phytopathogens simultaneously and the dual-use function of Promoting plant growth effect, this biological bactericide has wide spectrum, efficient feature, effectively can prevent and treat paddy rice, wheat, vegetables, fusarium wilt on the crops such as fruit tree, blight dis-ease, epidemic disease, banded sclerotial blight, false smut, rice blast, downy mildew, yellow rust, stalk break, head blight, anthracnose, gray mold, scab, leaf spot, bacterial wilt, canker, bacterial wilt of ginger and soil-borne disease soil treatment.
In the real process of agricultural production, a kind of medicament of use single continuously for a long time, pathogen will soon develop immunity to drugs, and causes preventive effect to reduce, and Pesticide use amount increases, the destruction of aggravation Practice for Pesticide Residue in Agricultural Products and ecotope.By carrying out composite with the mechanism of action diverse bactericide kind, being delay pathogen to develop immunity to drugs, expanding fungicidal spectrum, increasing the service life, reduce the effective way of Pesticide use amount.
In prior art, there is not yet about Zhongshengmycin and Shen piperazine mycin is composite and the relevant report applied.
Summary of the invention
For above-mentioned prior art, the invention provides a kind of biological antibiotic composition containing Zhongshengmycin, its active ingredient is Zhongshengmycin and Shen piperazine mycin, be biogenic pesticide, Zhongshengmycin, the Shen piperazine mycin mechanism of action are completely different, composite can to the effect of pathogen multidigit point, expand fungicidal spectrum, improve preventive effect, reduce Pesticide use amount, delay drug-fast generation.Present invention also offers the application that this contains the biological antibiotic composition of Zhongshengmycin.
The present invention is achieved by the following technical solutions:
Containing a biological antibiotic composition for Zhongshengmycin, active ingredient is Zhongshengmycin and Shen piperazine mycin, and the weight ratio of Zhongshengmycin and Shen piperazine mycin is 1:1 ~ 35, preferably, is 1:5 ~ 30.
Described containing in the biological antibiotic composition of Zhongshengmycin, can use and acceptable auxiliary material containing allowing in Pesticide Science.
Further, the described biological antibiotic composition containing Zhongshengmycin is made up of the component of following weight portion: Zhongshengmycin 1% ~ 80%, Shen piperazine mycin 1% ~ 80%, and surplus is allow in Pesticide Science to use and acceptable auxiliary material.
Allow in described Pesticide Science to use and acceptable auxiliary material be selected from solvent, dispersant, emulsifier, stabilizing agent, wetting agent, defoamer etc. and other contribute to the stable of active ingredient drug effect and performance allow make used additives.
The described biological antibiotic composition containing Zhongshengmycin, can be prepared into any one formulation of applicable agricultural production use by processing method well known in the prior art, preferred dosage form is: wetting powder, suspending agent, missible oil, aqueous emulsion, water dispersible granules, microemulsion.
Biological antibiotic composition containing Zhongshengmycin of the present invention, for preventing and treating the multiple diseases on fruit tree, vegetables, be particularly useful for soil-borne fungus, the bacterial diseases such as fruit root knot disease, bacterial wilt of ginger, root rot, simultaneously can various crop and the fruit diseases such as the water prevention rice sheath blight, downy mildew of garpe, alternaria leaf spot of apple.Preferably, when preventing and treating fruit root knot and being sick, Zhongshengmycin and the weight ratio both the piperazine mycin of Shen are good with 1:1 ~ 35, and 1:5 ~ 25 are better, and 1:10 is best.When preventing and treating bacterial wilt of ginger, Zhongshengmycin and the weight ratio both the piperazine mycin of Shen are good with 1:1 ~ 35, and 1:10 ~ 30 are better, and 1:20 is best.When preventing and treating root rot, Zhongshengmycin and the weight ratio both the piperazine mycin of Shen are good with 1:10 ~ 20, and 1:10 ~ 15 are better, and 1:10 is best.When preventing and treating alternaria leaf spot of apple, Zhongshengmycin and the weight ratio both the piperazine mycin of Shen are good with 1:1 ~ 15, and 1:5 ~ 15 are better, and 1:15 is best.When the water prevention rice sheath blight, Zhongshengmycin and the weight ratio both the piperazine mycin of Shen are good with 1:5 ~ 20, and 1:10 ~ 15 are better, and 1:10 is best.
Biological antibiotic composition containing Zhongshengmycin of the present invention, the mechanism of action of active ingredient Zhongshengmycin and Shen piperazine mycin is completely different, composite can to the effect of pathogen multidigit point, Synergistic, expand fungicidal spectrum, improve preventive effect, reduce Pesticide use amount, delay drug-fast generation, reduce resistance risk.
Biological antibiotic composition containing Zhongshengmycin of the present invention, finished product preparation form can provide, also can provide by unit dose form, and before using, directly mixing, is then watered mixing and is made into desired concn, and plant root is filled with and executed.During embody rule, also can be as used in combination in insecticide, growth regulator, soil conditioner, weed killer herbicide, nematocide etc. with other medicament.
Biological antibiotic composition containing Zhongshengmycin of the present invention, has the following advantages: 1, have synergistic function, improves control efficiency; 2, expand fungicidal spectrum, field diseases polyhybird occurs, and mixed use of Zhongshengmycin, Shen piperazine mycin doublely can control fungus and bacterium disease; 3, two kinds of active ingredient mechanisms of action are different, and mixture uses and delays the drug-fast generation of pathogen; 4, reduce formulation rate, reduce access times, reduce use cost.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.Medicine material, auxiliary material, bacterial classification etc. involved in following experiment, embodiment, unless otherwise noted, be existing conventional medicine raw material, auxiliary material, bacterial classification in prior art, buys obtain by routine business approach.Test method, preparation method etc. involved in following experiment, embodiment, unless otherwise noted, be routine techniques of the prior art.
Experiment Zhongshengmycin and the composite preventive and therapeutic effect to disease of Shen piperazine mycin are studied (Toxicity Determination)
One, reagent agent: zhongshengmycin bulk drug, the former medicine of Shen piperazine mycin, and the former medicine of kasugarnycin, the former medicine of jinggangmeisu, the former medicine of agricultural streptomycin.Former medicine is directly made into the mother liquor of 10000 μ g/mL by water-soluble solution, the volume fraction with 0.2% adds emulsifier Tween-80, preserves for subsequent use, be diluted to debita spissitudo during use with sterile water in refrigerator at 4 DEG C.
Two, for examination pathogen: bacteria pathogeny bacterium: falsesour cherry root carninomatosis bacterium (Agrobacterium tumefaciens (Smith & Town) Conn), ginger plague germ (Ralstonia solanacearum).Fungal pathogens: armillariella root rot of apple bacterium (Fusariumoxysporum), Rhizoctonia solani Kuhn (Rhizoctorzia solani), Alternaria mali (Alternaria mali).
Three, assay method:
(1) bacterial virulence assay method
Pathogenetic bacteria toxicity test for test material, adopts inhibition zone method to carry out toxicity test to Zhongshengmycin, Shen piperazine mycin and mixture preparation thereof with falsesour cherry root carninomatosis bacterium, ginger plague germ.
Single dose toxicity test method:
First reagent agent (comprising Zhongshengmycin, Shen piperazine mycin, kasugarnycin, jinggangmeisu, agricultural streptomycin) is mixed with 7 concentration gradients (Zhongshengmycin: 0.5,1.0,2.0,4.0,8.0,16.0,7 concentration gradients such as 32.0mg/kg; Shen piperazine mycin 1.0,2.0,4.0,8.0,16.0,32.0,7 concentration gradients such as 64.0mg/kg; Kasugarnycin 5.0,10.0,20.0,40.0,80.0,160.0,7 concentration gradients such as 320.0mg/kg; Jinggangmeisu 3.0,6.0,12.0,24.0,48.0,96.0,7 concentration gradients such as 192.0mg/kg; Agricultural streptomycin 5.0,10.0,20.0,40.0,80.0,160.0,7 concentration gradients such as 320.0mg/kg), the bacteria suspension 2mL (bacteria containing amount is about 106CFU/mL) getting diluted for use is in the culture dish of 90mm to diameter, add the NA medium 15mL (40 ~ 50 DEG C) that sterilizing is dissolved, abundant mixing, after its cooled and solidified, be that 1 hole is made a call at card punch 1/4 and 3/4 place on the diameter line of every ware medium of 5mm respectively with diameter, in every hole, add 50 μ L nutrient agar shop fixtures; After it solidifies, add the above-mentioned preparation medicament of 70 μ L respectively in wherein 1 hole of each culture dish, another 1 hole adds sterile water and compares, every concentration for the treatment of 5 repetition; After medium is cultivated 24 ~ 36h at 28 DEG C, measure antibacterial circle diameter, getting effective 3 mean values repeated is measurement result, calculate relative inhibition, after inhibiting rate is converted to probability value, calculate the toxicity regression formula of antibacterial probability value and reagent agent log concentration by method of least squares, obtain each medicament to concentration EC in effective suppression of bacterial strain
50.Test data carries out statistical analysis by Microsoft Excel 2003, DPS data processing work platform.
Mixed agent joint toxicity measuring:
On the basis of single dose toxicity test, press its EC respectively by supplying Zhongshengmycin, the Shen piperazine mycin of mixture
50value dosage in mass ratio example arranges 5: 1,2: 1,1: 1,1: 2,1: 5,1: 10,1: 15,1: 20,1: 25,1: 30,1: 35,1: 40,1: 45,1: 50 totally 14 proportionings respectively, be mixed with by different ratio the pastille NA medium that final mass concentration (referring to the gross mass of Zhongshengmycin, Shen piperazine mycin) is 2.0,4.0,8.0,16.0,32.0,64.0 and 128.0mg/kg7 series concentration, determine the inhibiting rate of medicament to growth of pathogenic bacteria.Often process (the every concentration level of every bacterial strain) and repeat 4 times.Suppress the linear regression analysis between probable value and drug concentration logarithm value by growth of pathogenic bacteria, obtain Mixed Pharmacy to concentration EC in effective suppression of pathogen
50value and CTC value, with the NA medium of not adding medicine in contrast.
(2) fungi toxicity test method
Disease fungus toxicity test for test material, adopts mycelial growth rate method to carry out toxicity test to Zhongshengmycin, Shen piperazine mycin and mixture preparation thereof with armillariella root rot of apple bacterium, Alternaria mali, Rhizoctonia solani Kuhn.
Single dose toxicity test method: by pathogen 26 DEG C of preculture 5d on PDA medium, bacterium cake is got same circumferentially the beating near colony edge with the card punch of diameter 7mm, be inoculated into and (comprise Zhongshengmycin, Shen piperazine mycin, kasugarnycin, jinggangmeisu containing different agents concentration, wherein, Zhongshengmycin: 0.5,1.0,2.0,4.0,8.0,16.0,7 concentration gradients such as 32.0mg/kg; Shen piperazine mycin 1.0,2.0,4.0,8.0,16.0,32.0,7 concentration gradients such as 64.0mg/kg; Kasugarnycin 5.0,10.0,20.0,40.0,80.0,160.0,7 concentration gradients such as 320.0mg/kg; Jinggangmeisu 3.0,6.0,12.0,24.0,48.0,96.0,7 concentration gradients such as 192.0mg/kg) culture medium flat plate on, every ware a slice, be placed in 26 DEG C of insulating boxs and cultivate 4d, the colony growth diameter of each process is measured by right-angled intersection method, take clear water as contrast, every concentration process repeats 4 times.Measure the radial linear amount of growth of bacterium colony, determine the inhibiting rate of medicament to colony growth.Suppress the linear regression analysis between probable value and drug concentration logarithm value by mycelial growth, obtain each medicament to concentration (EC in effective suppression of bacterial strain
50value).
Mixed agent joint toxicity measuring:
On the basis of single dose toxicity test, press its EC respectively by supplying Zhongshengmycin, the Shen piperazine mycin of mixture
50value dosage arranges 5: 1,2: 1,1: 1,1: 2,1: 5,1: 10,1: 15,1: 20,1: 25,1: 30,1: 35,1: 40,1: 45,1: 50 totally 14 proportionings by weight proportion respectively, by different ratio be mixed with final mass concentration (referring to the gross mass of Zhongshengmycin, Shen piperazine mycin) be 0.25,0.5,1.0,2.0,4.0,8.0, the pastille PDA medium of 16.0mg/kg 7 series concentration, determine the inhibiting rate of medicament to colony growth.Often process (the every concentration level of every bacterial strain) and repeat 4 times.Suppress the linear regression analysis between probable value and drug concentration logarithm value by mycelial growth, obtain Mixed Pharmacy to concentration EC in effective suppression of bacterial strain
50value and CTC value, with the PDA medium of not adding medicine in contrast.
(3) data statistic analysis
Test data carries out statistical analysis by Microsoft Excel 2003, DPS data processing work platform, calculates the EC of often kind of medicament
50value, 95% confidence limit.Utilize the abundant method of Sun Yun (1960) to calculate the co-toxicity coefficient of mixed agent, evaluate the synergistic effect of mixed agent according to co-toxicity coefficient size.
According to each medicament inhibiting rate of following formulae discovery.
Developing restraint rate (%)=[(contrast antibacterial circle diameter-process antibacterial circle diameter)/(contrast antibacterial circle diameter-5mm punching diameter)] × 100
Developing restraint rate (%)=[(contrast colony diameter-process colony diameter)/(contrast colony diameter-bacterium cake diameter)] × 100
Toxicity index TI=(standard agent EC
50/ reagent agent EC
50) × 100
The actual toxicity index ATI=of mixed agent (standard agent EC
50/ mixture preparation EC
50) × 100
TI × the PB (PA and PB is respectively the percentage composition of active ingredient in mixed agent) of TI × PA+ single dose B of the theoretical toxicity index TTI=single dose A of mixed agent
Theoretical toxicity index TTI × 100 of the actual toxicity index ATI/ mixed agent of co-toxicity coefficient CTC=mixed agent
Synergistic effect judges: CTC >=120, have synergistic effect; 80 < CTC < 120 are summation action; CTC≤80 are antagonism.
(4) results and analysis: as shown in table 1 ~ table 10.
Table 15 kinds of medicaments are to the toxicity test result of falsesour cherry root carninomatosis bacterium
Table 2 Zhongshengmycin and Shen piperazine mycin different proportion mixture are to the toxicity test result of falsesour cherry root carninomatosis bacterium
From table 1 ~ 2, Zhongshengmycin, Shen piperazine mycin and mixture preparation thereof, to falsesour cherry root carninomatosis bacterium, there is good inhibitory action, when wherein the proportioning of Zhongshengmycin and Shen piperazine mycin is between 1:1 ~ 1:35, to the co-toxicity coefficient for examination pathogen all more than 120, show that these two kinds of bactericide have good synergistic function under this proportioning, especially proportioning synergistic effect between 1:5 ~ 1:25 is more remarkable.
Table 35 kinds of medicaments are to the toxicity test result of ginger plague germ
Table 4 Zhongshengmycin and Shen piperazine mycin different proportion mixture are to the toxicity test result of ginger plague germ
From table 3 ~ 4, Zhongshengmycin, Shen piperazine mycin and mixture preparation thereof, to ginger plague germ, there is good inhibitory action, when wherein the proportioning of Zhongshengmycin and Shen piperazine mycin is between 1:1 ~ 1:35, to the co-toxicity coefficient for examination pathogen all more than 120, show that these two kinds of bactericide have good synergistic function under this proportioning, especially proportioning synergistic effect between 1:10 ~ 1:30 is more remarkable.
Table 55 kinds of medicaments are to the toxicity test result of armillariella root rot of apple bacterium
Table 6 Zhongshengmycin and Shen piperazine mycin different proportion mixture are to the toxicity test result of armillariella root rot of apple bacterium
From table 5 ~ 6, Zhongshengmycin, Shen piperazine mycin and mixture preparation thereof, to armillariella root rot of apple bacterium, there is good inhibitory action, when wherein the proportioning of Zhongshengmycin and Shen piperazine mycin is between 1:10 ~ 1:20, to the co-toxicity coefficient for examination pathogen all more than 120, show that these two kinds of bactericide have good synergistic function under this proportioning, especially proportioning synergistic effect between 1:10 ~ 1:15 is more remarkable.
Table 75 kinds of medicaments are to the toxicity test result of Alternaria mali
Table 8 Zhongshengmycin and Shen piperazine mycin different proportion mixture are to the toxicity test result of Alternaria mali
From table 7 ~ 8, Zhongshengmycin, Shen piperazine mycin and mixture preparation thereof, to Alternaria mali, there is good inhibitory action, when wherein the proportioning of Zhongshengmycin and Shen piperazine mycin is between 1:1 ~ 1:15, to the co-toxicity coefficient for examination pathogen substantially all more than 120, show that these two kinds of bactericide have synergistic function under this proportioning, especially proportioning synergistic effect between 1:5 ~ 1:15 is comparatively remarkable.
Table 95 kinds of medicaments are to the toxicity test result of Rhizoctonia solani Kuhn
Table 10 Zhongshengmycin and Shen piperazine mycin different proportion mixture are to the toxicity test result of Rhizoctonia solani Kuhn
From table 9 ~ 10, Zhongshengmycin, Shen piperazine mycin and mixture preparation thereof, to Rhizoctonia solani Kuhn, there is good inhibitory action, when wherein the proportioning of Zhongshengmycin and Shen piperazine mycin is between 1:5 ~ 1:20, to the co-toxicity coefficient for examination pathogen all more than 120, show that these two kinds of bactericide have good synergistic function under this proportioning, and synergistic effect is more remarkable.
Embodiment 1 5% Zhongshengmycin Shen piperazine mycin wetting powder
Component (each components based on weight percentage) composed as follows:
Preparation method is: above-mentioned each active component, various auxiliary agent and filler are fully mixed in proportion, and mixture, through ultra-fine pulverizer disintegrating, obtains 5% Zhongshengmycin Shen piperazine mycin wetting powder.
This embodiment is applied to prevention and control falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters their early stage, and variable concentrations is watered draws furrow irrigation root, and every 15 days once, connects and execute 3 times; The water prevention rice sheath blight, alternaria leaf spot of apple, enter their early stage, and variable concentrations foliar spray is prevented and treated, and once, the season of growth uses 2 ~ 3 times in medication in every 10 ~ 15 days.Control efficiency sees the following form 11, and (composition of its auxiliary material is with embodiment 1, and 1% described Shen piperazine mycin SC, the composition of its auxiliary material is with embodiment 2 for note: in table 11 ~ table 16,3% described Zhongshengmycin WP.Each extension rate in table, for the solid dosage forms such as wetting powder, water dispersible granules, refers to quality multiple, for other formulation, refers to volume multiple).
Table 11 Zhongshengmycin prevents and treats the field efficacy of falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, rice sheath blight disease, alternaria leaf spot of apple from the Shen different preparation of piperazine mycin
Can be found out by table 11, after Zhongshengmycin and Shen piperazine mycin mixture, synergistic effect is obvious, and to the control efficiency of five kinds of diseases significantly better than single dose, and effective ingredient consumption obviously reduces.
Embodiment 2 25% Zhongshengmycin Shen piperazine mycin suspending agent
Component (each components based on weight percentage) composed as follows:
Preparation method is: above-mentioned each active component, various auxiliary agent and water are fully mixed in proportion, drops into sand mill, is ground to particle diameter 5 μm, 25% Zhongshengmycin Shen piperazine mycin suspending agent.
This embodiment is applied to prevention and control falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters their early stage, and variable concentrations is watered draws furrow irrigation root, and every 15 days once, connects and execute 3 times; The water prevention rice sheath blight, alternaria leaf spot of apple, enter their early stage, and variable concentrations foliar spray is prevented and treated, and once, the season of growth uses 2 ~ 3 times in medication in every 10 ~ 15 days.Control efficiency sees the following form 12.
Table 12 Zhongshengmycin prevents and treats the field efficacy of falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, rice sheath blight disease, alternaria leaf spot of apple from the Shen different preparation of piperazine mycin
Can be found out by table 12, after Zhongshengmycin and Shen piperazine mycin mixture, synergistic effect is obvious, and to the control efficiency of five kinds of diseases significantly better than single dose, and effective ingredient consumption obviously reduces.
Embodiment 3 11% Zhongshengmycin Shen piperazine mycin missible oil
Component (each components based on weight percentage) composed as follows:
Preparation method is: above-mentioned each active component, various auxiliary agent and water are fully mixed in proportion, add in mixing kettle, the 11% Zhongshengmycin Shen piperazine mycin missible oil stirred.
This embodiment is applied to prevention and control falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters their early stage, and variable concentrations is watered draws furrow irrigation root, and every 15 days once, connects and execute 3 times; The water prevention rice sheath blight, alternaria leaf spot of apple, enter their early stage, and variable concentrations foliar spray is prevented and treated, and once, the season of growth uses 2 ~ 3 times in medication in every 10 ~ 15 days.Control efficiency sees the following form 13.
Table 13 Zhongshengmycin prevents and treats the field efficacy of falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, rice sheath blight disease, alternaria leaf spot of apple from the Shen different preparation of piperazine mycin
Can be found out by table 13, after Zhongshengmycin and Shen piperazine mycin mixture, synergistic effect is obvious, and to the control efficiency of five kinds of diseases significantly better than single dose, and effective ingredient consumption obviously reduces.
Embodiment 4:16% Zhongshengmycin Shen piperazine mycin aqueous emulsion
Component (each components based on weight percentage) composed as follows:
Preparation method is: above-mentioned each active component, various auxiliary agent are fully mixed in proportion, and make to be dissolved into all and oil phase, under high-speed stirred, oil phase is mixed with water, obtains 16% Zhongshengmycin Shen piperazine mycin aqueous emulsion.
This embodiment is applied to prevention and control falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters their early stage, and variable concentrations is watered draws furrow irrigation root, and every 15 days once, connects and execute 3 times; The water prevention rice sheath blight, alternaria leaf spot of apple, enter their early stage, and variable concentrations foliar spray is prevented and treated, and once, the season of growth uses 2 ~ 3 times in medication in every 10 ~ 15 days.Control efficiency sees the following form 14.
Table 14 Zhongshengmycin prevents and treats the field efficacy of falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, rice sheath blight disease, alternaria leaf spot of apple from the Shen different preparation of piperazine mycin
Can be found out by table 14, after Zhongshengmycin and Shen piperazine mycin mixture, synergistic effect is obvious, and to the control efficiency of five kinds of diseases significantly better than single dose, and effective ingredient consumption obviously reduces.
Embodiment 5:6% Zhongshengmycin Shen piperazine mycin water dispersible granules
Component (each components based on weight percentage) composed as follows:
Preparation method is: above-mentioned each active component, Negel formaldehyde condensation products, wetting agent, disintegrant, filler are fully mixed in proportion, particle diameter 5 μm is crushed to through airslide disintegrating mill, obtain female powder, female powder is fully mixed rear granulation with sodium lignin sulfonate, dries, sieves, thus obtained 6% Zhongshengmycin Shen piperazine mycin water-dispersible granules.
This embodiment is applied to prevention and control falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters their early stage, and variable concentrations is watered draws furrow irrigation root, and every 15 days once, connects and execute 3 times; The water prevention rice sheath blight, alternaria leaf spot of apple, enter their early stage, and variable concentrations foliar spray is prevented and treated, and once, the season of growth uses 2 ~ 3 times in medication in every 10 ~ 15 days.Control efficiency sees the following form 15.
Table 15 Zhongshengmycin prevents and treats the field efficacy of falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, rice sheath blight disease, alternaria leaf spot of apple from the Shen different preparation of piperazine mycin
Can be found out by table 15, after Zhongshengmycin and Shen piperazine mycin mixture, synergistic effect is obvious, and to the control efficiency of five kinds of diseases significantly better than single dose, and effective ingredient consumption obviously reduces.
Embodiment 6:26% Zhongshengmycin Shen piperazine mycin microemulsion
Component (each components based on weight percentage) composed as follows:
Preparation method is: above-mentioned each active component, various auxiliary agent are fully mixed in proportion, and make to be dissolved into all and oil phase, under high-speed stirred, oil phase is mixed with water, obtains 26% Zhongshengmycin Shen piperazine mycin microemulsion.
This embodiment is applied to prevention and control falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters their early stage, and variable concentrations is watered draws furrow irrigation root, and every 15 days once, connects and execute 3 times; The water prevention rice sheath blight, alternaria leaf spot of apple, enter their early stage, and variable concentrations foliar spray is prevented and treated, and once, the season of growth uses 2 ~ 3 times in medication in every 10 ~ 15 days.Control efficiency sees the following form 16.
Table 16 Zhongshengmycin prevents and treats the field efficacy of falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, rice sheath blight disease, alternaria leaf spot of apple from the Shen different preparation of piperazine mycin
Can be found out by table 16, after Zhongshengmycin and Shen piperazine mycin mixture, synergistic effect is obvious, and to the control efficiency of five kinds of diseases significantly better than single dose, and effective ingredient consumption obviously reduces.
Claims (10)
1., containing a biological antibiotic composition for Zhongshengmycin, it is characterized in that: active ingredient is Zhongshengmycin and Shen piperazine mycin, and the weight ratio of Zhongshengmycin and Shen piperazine mycin is 1:1 ~ 35.
2. the biological antibiotic composition containing Zhongshengmycin according to claim 1, it is characterized in that: the described biological antibiotic composition containing Zhongshengmycin is made up of the component of following weight portion: Zhongshengmycin 1% ~ 80%, Shen piperazine mycin 1% ~ 80%, surplus is allow in Pesticide Science to use and acceptable auxiliary material.
3. the biological antibiotic composition containing Zhongshengmycin according to claim 1, is characterized in that: the formulation of the described biological antibiotic composition containing Zhongshengmycin is wetting powder, suspending agent, missible oil, aqueous emulsion, water dispersible granules or microemulsion.
4. the bactericidal composition containing Zhongshengmycin according to any one of claims 1 to 3, it is characterized in that: the formulation of the described biological antibiotic composition containing Zhongshengmycin is wetting powder, be made up of the component of following percetage by weight: Zhongshengmycin 1%, Shen piperazine mycin 4%, Negel formaldehyde condensation products 3%, sodium lignin sulfonate 4%, lauryl sodium sulfate 4%, White Carbon black 20%, surplus is kaolin.
5. the bactericidal composition containing Zhongshengmycin according to any one of claims 1 to 3, it is characterized in that: the formulation of the described biological antibiotic composition containing Zhongshengmycin is suspending agent, be made up of the component of following percetage by weight: Zhongshengmycin 1%, Shen piperazine mycin 24%, Negel formaldehyde condensation products 6%, castor oil polyoxyethylene ether 4%, fatty alcohol-polyoxyethylene ether 6%, sodium methylene bis-naphthalene sulfonate 5%, xanthans 3%, glycerine 5%, surplus is water.
6. the bactericidal composition containing Zhongshengmycin according to any one of claims 1 to 3, it is characterized in that: the formulation of the described biological antibiotic composition containing Zhongshengmycin is missible oil, be made up of the component of following percetage by weight: Zhongshengmycin 1%, Shen piperazine mycin 10%, cyclohexane 27%, agricultural emulsifier NO.600 4%, castor oil polyethylene glycol condensation 7%, glycerine 5%, surplus is dimethylbenzene.
7. according to any one of claims 1 to 3 containing the bactericidal composition of Zhongshengmycin, it is characterized in that: the formulation of the described biological antibiotic composition containing Zhongshengmycin is aqueous emulsion or water dispersible granules or microemulsion;
When formulation is aqueous emulsion, be made up of the component of following percetage by weight: Zhongshengmycin 1%, Shen piperazine mycin 15%, cyclohexane 22%, agricultural emulsifier NO.600 4%, castor oil polyethylene glycol condensation 4%, Negel formaldehyde condensation products 6%, excess water;
When formulation is water dispersible granules, be made up of the component of following percetage by weight: Zhongshengmycin 1%, Shen piperazine mycin 5%, Negel formaldehyde condensation products 6%, sodium lignin sulfonate 5%, sodium methylene bis-naphthalene sulfonate 3%, ammonium sulfate 4%, White Carbon black 30%, surplus is calcium carbonate;
When formulation is microemulsion, be made up of the component of following percetage by weight: Zhongshengmycin 1%, Shen piperazine mycin 25%, cyclohexane (solvent) 17%, agricultural emulsifier NO.600 (emulsifier) 4%, castor oil polyethylene glycol condensation (emulsifier) 6%, isopropyl alcohol (solvent) 17%, surplus is water.
8. the application in the disease of the biological antibiotic composition containing Zhongshengmycin according to any one of claim 1 ~ 7 on control fruit tree, vegetables, crop.
9. application according to claim 8, is characterized in that: described disease is selected from fruit root knot disease, bacterial wilt of ginger, root rot, rice sheath blight disease, downy mildew of garpe, alternaria leaf spot of apple.
10. application according to claim 9, is characterized in that: when preventing and treating fruit root knot and being sick, Zhongshengmycin and the weight ratio both the piperazine mycin of Shen are 1:1 ~ 35; When preventing and treating bacterial wilt of ginger, Zhongshengmycin and the weight ratio both the piperazine mycin of Shen are 1:1 ~ 35; When preventing and treating root rot, Zhongshengmycin and the weight ratio both the piperazine mycin of Shen are 1:10 ~ 20; When preventing and treating alternaria leaf spot of apple, Zhongshengmycin and the weight ratio both the piperazine mycin of Shen are 1:1 ~ 15; When the water prevention rice sheath blight, Zhongshengmycin and the weight ratio both the piperazine mycin of Shen are 1:5 ~ 20.
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| CN108077314A (en) * | 2016-11-22 | 2018-05-29 | 上海农乐生物制品股份有限公司 | A kind of shenqinmycin waxy Bacillus compound water dispersible granule and its preparation and application |
| CN110731337A (en) * | 2019-10-28 | 2020-01-31 | 浙江省桐庐汇丰生物科技有限公司 | plant disease preventing and treating composition and its application |
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| CN113243393A (en) * | 2020-07-08 | 2021-08-13 | 西北民族大学 | Preparation method and application of nematophagous fungus chlamydospore wettable powder |
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| CN116267988A (en) * | 2023-04-03 | 2023-06-23 | 贵州省植物保护研究所 | Pesticide composition containing sodium chloride and Zhongshengmycin, and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108077314A (en) * | 2016-11-22 | 2018-05-29 | 上海农乐生物制品股份有限公司 | A kind of shenqinmycin waxy Bacillus compound water dispersible granule and its preparation and application |
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| CN110731337B (en) * | 2019-10-28 | 2021-04-20 | 浙江省桐庐汇丰生物科技有限公司 | Composition for preventing and treating plant diseases and application thereof |
| CN111226966A (en) * | 2020-03-05 | 2020-06-05 | 福建省农业科学院植物保护研究所 | Suspended seed coating agent for preventing and treating peanut diseases and insect pests and preparation method thereof |
| CN113243393A (en) * | 2020-07-08 | 2021-08-13 | 西北民族大学 | Preparation method and application of nematophagous fungus chlamydospore wettable powder |
| CN113243393B (en) * | 2020-07-08 | 2022-06-07 | 延安大学 | Preparation method and application of nematophagous fungus chlamydospore wettable powder |
| CN114982770A (en) * | 2022-06-16 | 2022-09-02 | 江西省农业科学院植物保护研究所 | Composition for preventing and treating sesame wilt and application thereof |
| CN116267988A (en) * | 2023-04-03 | 2023-06-23 | 贵州省植物保护研究所 | Pesticide composition containing sodium chloride and Zhongshengmycin, and preparation method and application thereof |
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