CN104782641B - Containing biological antibiotic composition and the application thereof of Zhongshengmycin - Google Patents
Containing biological antibiotic composition and the application thereof of Zhongshengmycin Download PDFInfo
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Abstract
The invention discloses a kind of biological antibiotic composition containing Zhongshengmycin, active ingredient is Zhongshengmycin and Shen piperazine mycin, the weight ratio of Zhongshengmycin and Shen piperazine mycin is 1:1～35, can also contain in Pesticide Science and allow to use and acceptable auxiliary material, formulation can be wettable powder, suspending agent, missible oil, aqueous emulsion, water dispersible granules or microemulsion. Biological antibiotic composition containing Zhongshengmycin of the present invention, the mechanism of action of active ingredient Zhongshengmycin and Shen piperazine mycin is completely different, composite can be to the effect of pathogen multidigit point, Synergistic, expand fungicidal spectrum, improve preventive effect, reduce agricultural chemicals use amount, delay drug-fast generation, reduce resistance risk. Bactericidal composition of the present invention is for preventing and treating the multiple diseases on fruit tree, vegetables, be particularly useful for soil-borne fungus, the bacterial diseases such as fruit root knot disease, bacterial wilt of ginger, root rot, can prevent and treat crop and the fruit diseases such as rice sheath blight disease, downy mildew of garpe, alternaria leaf spot of apple simultaneously.
Description
Technical field
The present invention relates to a kind of biological antibiotic composition and application thereof containing Zhongshengmycin, in biological pesticide Prevention Technique field.
Background technology
At present, chemical prevention is the important measures of controlling day by day serious plant disease, has that instant effect, fungicidal spectrum are wide, costThe advantage such as low, easy to use, to ensureing that agricultural production brought into play important function, but long-term a large amount of use of chemical bactericide causesA series of serious ecological problems and the social concerns such as the agricultural chemicals resistance to the action of a drug, residues of pesticides, environmental pollution, agricultural product security. In recent yearsCome, along with the quality safety of people to agricultural product and the concern of problem of environmental pollution, the use of chemical bactericide is subject to more and more tighterThe restriction of lattice. Therefore, people start to be more conceived to more low toxicity, environmental protection, free of contamination biogenic pesticide. Biogenic pesticideBe to utilize the antagonist properties between microorganism to prevent and treat pathogen, this is more efficient and environmental protection than existing chemical pesticide. At present, openSending out biogenic pesticide new varieties and complex preparation is more and more subject to people's attention.
Zhongshengmycin is a kind of novel agricultural antibiotic, and its fungicidal spectrum is wide, and bacterial disease and fungal disease to crops are equalThere is good preventive effect, can anti-bacteria bacterium protein synthetic, cause thalline death; Fungus spore germination is had stronglyInhibitory action, cause plasm cohesion in hyphal cell, change the form of thread mycelia.
Shen piperazine mycin is a kind of antibiotic through biological culture secretion by Pseudomonas fluorescens, and main component is phenazine-1-carboxylic acid(phenazine-1-carboxylicacid), there is the dual of wide spectrum Suppressing phytopathogens Promoting plant growth effect simultaneouslyFunction, this biological bactericide has wide spectrum, efficient feature, can effectively prevent and treat on the crops such as paddy rice, wheat, vegetables, fruit treeDroop, climing rot, epidemic disease, banded sclerotial blight, false smut, rice blast, downy mildew, stripe rust, sclerotiniose, head blight,Anthracnose, gray mold, scab, leaf spot, bacterial wilt, canker, bacterial wilt of ginger and soil-borne disease soil treatment.
In the real process of agricultural production, a kind of medicament of long-term continuous single use, pathogen will soon develop immunity to drugs,Cause preventive effect to reduce, agricultural chemicals use amount increases, the destruction of aggravation Practice for Pesticide Residue in Agricultural Products and ecological environment. By with the mechanism of actionDiverse bactericide kind is carried out composite, is to delay pathogen to develop immunity to drugs, and expands fungicidal spectrum, increase the service life,Reduce the effective way of agricultural chemicals use amount.
In prior art, there is not yet about Zhongshengmycin and Shen piperazine mycin is composite and application relevant report.
Summary of the invention
For above-mentioned prior art, the invention provides a kind of biological antibiotic composition containing Zhongshengmycin, during its active ingredient isRaw rhzomorph and Shen piperazine mycin, be biogenic pesticide, and Zhongshengmycin, the Shen piperazine mycin mechanism of action are completely different, composite can be to diseaseThe effect of former bacterium multidigit point, expands fungicidal spectrum, improves preventive effect, reduces agricultural chemicals use amount, delays drug-fast generation. The present invention alsoThis application containing the biological antibiotic composition of Zhongshengmycin is provided.
The present invention is achieved by the following technical solutions:
Containing a biological antibiotic composition for Zhongshengmycin, active ingredient is Zhongshengmycin and Shen piperazine mycin, Zhongshengmycin and Shen piperazineThe weight ratio of mycin is 1:1～35, preferred, is 1:5～30.
Described containing in the biological antibiotic composition of Zhongshengmycin, can contain in Pesticide Science and allow to use and acceptable auxiliary material.
Further, the described biological antibiotic composition that contains Zhongshengmycin is made up of the component of following weight portion: Zhongshengmycin1%～80%, Shen piperazine mycin 1%～80%, surplus is in Pesticide Science, to allow to use and acceptable auxiliary material.
In described Pesticide Science, allow to use and acceptable auxiliary material be selected from solvent, dispersant, emulsifying agent, stabilizing agent, wetting agent,Defoamer etc. and other contribute to active ingredient drug effect stable and performance allow to make used additives.
The described biological antibiotic composition containing Zhongshengmycin, can be prepared into and be applicable to agricultural by processing method well known in the prior artAny one formulation of production and application, preferred dosage form is: wettable powder, suspending agent, missible oil, aqueous emulsion, water dispersible granules,Microemulsion.
Biological antibiotic composition containing Zhongshengmycin of the present invention, for preventing and treating the multiple diseases on fruit tree, vegetables, especially applicableIn soil-borne fungus, bacterial diseases such as fruit root knot disease, bacterial wilt of ginger, root rot, can prevent and treat rice sheath blight disease, grape simultaneouslyThe various crop such as downy mildew, alternaria leaf spot of apple and fruit diseases. Preferably, in the time that control fruit root knot is sick, ZhongshengmycinTaking 1:1～35 as good, 1:5～25 are better, 1:10 the best with the two weight ratio of Shen piperazine mycin. In the time of control bacterial wilt of ginger, middle lifeThe two weight ratio of rhzomorph and Shen piperazine mycin is taking 1:1～35 as good, and 1:10～30 are better, 1:20 the best. In the time preventing and treating root rot,The two weight ratio of Zhongshengmycin and Shen piperazine mycin is taking 1:10～20 as good, and 1:10～15 are better, 1:10 the best. At control appleWhen spot defoliation, the two weight ratio of Zhongshengmycin and Shen piperazine mycin is taking 1:1～15 as good, and 1:5～15 are better, 1:15 the best.In the time preventing and treating rice sheath blight disease, the two weight ratio of Zhongshengmycin and Shen piperazine mycin is taking 1:5～20 as good, and 1:10～15 are better,1:10 the best.
Biological antibiotic composition containing Zhongshengmycin of the present invention, the mechanism of action of active ingredient Zhongshengmycin and Shen piperazine mycin is completeDifference, composite can be to the effect of pathogen multidigit point, Synergistic, expands fungicidal spectrum, improves preventive effect, reduces agricultural chemicals use amount,Delay drug-fast generation, reduce resistance risk.
Biological antibiotic composition containing Zhongshengmycin of the present invention, can finished product preparation form provide, also can single dose form provide,Before using, directly mixing, is then watered to mix and is made into desired concn, and the filling of plant root is executed. When concrete application, also can with otherMedicament uses as pesticide, growth regulator, soil conditioner, herbicide, nematicide etc. mix.
Biological antibiotic composition containing Zhongshengmycin of the present invention, has the following advantages: 1, have synergistic function, improvePrevention effect; 2, expand fungicidal spectrum, field disease polyhybird occurs, and Zhongshengmycin, the mixed use of Shen piperazine mycin can doublely be controlled fungiAnd bacteriosis; 3, two kinds of active ingredient mechanism of action differences, mixture is used and delays the drug-fast generation of pathogen; 4, reduceFormulation rate, reduces access times, reduces use cost.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated. Related medicine material, auxiliary in following experiment, embodimentMaterial, bacterial classification etc., unless otherwise noted, be existing conventional medicine raw material, auxiliary material, bacterial classification in prior art, can be by normalRule commercial sources is bought and is obtained. Related test method, preparation method etc. in following experiment, embodiment, unless otherwise noted,Be routine techniques of the prior art.
The composite research of the preventive and therapeutic effect to disease of experiment Zhongshengmycin and Shen piperazine mycin (Toxicity Determination)
One, reagent agent: the former medicine of Zhongshengmycin, the former medicine of Shen piperazine mycin, and the former medicine of kasugarnycin, the former medicine of jinggangmeisu, agricultureWith the former medicine of streptomysin. The direct water of former medicine dissolves the mother liquor that is made into 10000 μ g/mL, adds emulsification with 0.2% volume fractionAgent Tween-80 preserves for subsequent usely in refrigerator at 4 DEG C, when use, be diluted to debita spissitudo with sterilized water.
Two, for examination pathogen: bacteria pathogeny bacterium: falsesour cherry root carninomatosis bacterium (Agrobacteriumtumefaciens (Smith&Town)Conn), ginger plague germ (Ralstoniasolanacearum). Fungal pathogens: armillariella root rot of apple bacterium (FusariumOxysporum), Rhizoctonia solani Kuhn (Rhizoctorziasolani), Alternaria mali (Alternariamali).
Three, assay method:
(1) bacterial virulence assay method
Pathogenetic bacteria toxicity test is taking falsesour cherry root carninomatosis bacterium, ginger plague germ as test material, adopt inhibition zone method to Zhongshengmycin,Shen piperazine mycin and mixture preparation thereof carry out toxicity test.
Single dose toxicity test method:
First reagent agent (comprising Zhongshengmycin, Shen piperazine mycin, kasugarnycin, jinggangmeisu, agricultural streptomycin) is mixed with to 70.5,1.0,2.0,4.0,8.0,16.0,7 concentration gradients such as 32.0mg/kg individual concentration gradient (Zhongshengmycin:; Shen7 concentration gradients such as piperazine mycin 1.0,2.0,4.0,8.0,16.0,32.0,64.0mg/kg; Kasugarnycin 5.0,10.0,20.0,40.0,80.0,160.0,7 concentration gradients such as 320.0mg/kg; Jinggangmeisu 3.0,6.0,12.0,24.0,48.0,96.0,7 concentration gradients such as 192.0mg/kg; Agricultural streptomycin 5.0,10.0,20.0,40.0,80.0,160.0,7 concentration gradients such as 320.0mg/kg), the bacteria suspension 2mL (bacteria containing amount is about 106CFU/mL) that gets diluted for use to diameter isIn the culture dish of 90mm, the NA culture medium 15mL (40～50 DEG C) that adds sterilizing to dissolve, fully mixes, and treats that it is cooling solidifyingGu after, 1 hole is made a call to respectively at the card punch that is 5mm with diameter 1/4 and 3/4 place on the diameter line of every ware culture medium, each in every holeAdd 50 μ L nutrient agar shop fixtures; After it solidifies, add respectively 70 μ L above-mentioned in wherein 1 hole of each culture dishPreparation medicament, another 1 hole adds sterilized water to compare, 5 repetitions of every concentration for the treatment of; After culture medium is cultivated to 24～36h at 28 DEG C,Measure antibacterial circle diameter, the mean value of getting effective 3 repetitions is measurement result, calculate relative inhibition, after inhibiting rate is turnedBe changed to probability value, calculate the toxicity regression formula of antibacterial probability value and reagent agent concentration logarithm by least square method, obtain each medicineAgent is to concentration EC in effective inhibition of bacterial strain50. Test data is flat by MicrosoftExcel2003, DPS data processing workPlatform carries out statistical analysis.
Mixed agent joint toxicity measuring:
On the basis of single dose toxicity test, the Zhongshengmycin for mixture, Shen piperazine mycin are pressed respectively to its EC50Value dosage in mass ratioExample arranges respectively 5: 1,2: 1,1: 1,1: 2,1: 5,1: 10,1: 15,1: 20,1: 25,1: 30,1: 35,1: 40,1: 45,1: 50 totally 14 proportionings, by different proportionings be mixed with final mass concentration (refer to Zhongshengmycin,The gross mass of Shen piperazine mycin) be 2.0,4.0,8.0,16.0,32.0,64.0 and the pastille of 128.0mg/kg7 series concentrationNA culture medium, determines the inhibiting rate of medicament to pathogen growth. Every processing (the every concentration level of every bacterial strain) repeats 4 times. By diseaseFormer bacteria growing suppresses the linear regression analysis between probable value and drug concentration logarithm value, obtain Mixed Pharmacy to pathogen effectivelyConcentration EC in inhibition50Value and CTC value, with the NA culture medium of adding medicine not in contrast.
(2) fungi toxicity test method
Disease fungus toxicity test, taking armillariella root rot of apple bacterium, Alternaria mali, Rhizoctonia solani Kuhn as test material, is adoptedZhongshengmycin, Shen piperazine mycin and mixture preparation thereof are carried out to toxicity test by mycelial growth rate method.
Single dose toxicity test method: by pathogen 26 DEG C of preculture 5d on PDA culture medium, use the card punch of diameter 7mm closeOn the same circumference of colony edge, beat and get bacterium cake, be inoculated into and contain different drug concentrations and (comprise Zhongshengmycin, Shen piperazine mycin, springThunder mycin, jinggangmeisu, wherein, 0.5,1.0,2.0,4.0,8.0,16.0,7 of 32.0mg/kg etc. Zhongshengmycin:Concentration gradient; 7 concentration gradients such as Shen piperazine mycin 1.0,2.0,4.0,8.0,16.0,32.0,64.0mg/kg; Spring thunder is mould7 concentration gradients such as element 5.0,10.0,20.0,40.0,80.0,160.0,320.0mg/kg; Jinggangmeisu 3.0,6.0,12.0,24.0,48.0,96.0,7 concentration gradients such as 192.0mg/kg) culture medium flat plate on, every ware a slice, is placed inIn 26 DEG C of insulating boxs, cultivate 4d, measure the colony growth diameter of each processing by right-angled intersection method, taking clear water as contrast, every concentration placeReason repeats 4 times. Measure the radially linear increment of bacterium colony, determine the inhibiting rate of medicament to colony growth. Suppress general by mycelial growthLinear regression analysis between rate value and drug concentration logarithm value, obtains each medicament to concentration (EC in effective inhibition of bacterial strain50Value).
Mixed agent joint toxicity measuring:
On the basis of single dose toxicity test, the Zhongshengmycin for mixture, Shen piperazine mycin are pressed respectively to its EC50Value dosage by weightExample arranges respectively 5: 1,2: 1,1: 1,1: 2,1: 5,1: 10,1: 15,1: 20,1: 25,1: 30,1: 35,1: 40,1: 45,1: 50 totally 14 proportionings, by different proportionings be mixed with final mass concentration (refer to Zhongshengmycin,The gross mass of Shen piperazine mycin) be 0.25,0.5,1.0,2.0,4.0,8.0, the pastille PDA of a 16.0mg/kg7 series concentrationCulture medium, determines the inhibiting rate of medicament to colony growth. Every processing (the every concentration level of every bacterial strain) repeats 4 times. Pass through mycelial growthSuppress the linear regression analysis between probable value and drug concentration logarithm value, obtain Mixed Pharmacy to concentration in effective inhibition of bacterial strainEC50Value and CTC value, with the PDA culture medium of adding medicine not in contrast.
(3) data statistic analysis
Test data is carried out statistical analysis by MicrosoftExcel2003, DPS data processing work platform, calculates every kindThe EC of medicament50Value, 95% confidence limit. Utilize the abundant method of Sun Yun (1960) to calculate the co-toxicity coefficient of mixed agent, according to co-toxicity coefficient sizeEvaluate the synergistic effect of mixed agent.
Calculate each medicament inhibiting rate according to following formula.
Suppress growth rate (%)=[(contrast antibacterial circle diameter-processing antibacterial circle diameter)/(contrast antibacterial circle diameter-5mm punchingDiameter)] × 100
Suppress growth rate (%)=[(contrast colony diameter-processing colony diameter)/(contrast colony diameter-bacterium cake diameter)]×100
Toxicity index TI=(standard medicament EC50/ reagent agent EC50)×100
The actual toxicity index ATI=of mixed agent (standard medicament EC50/ mixture preparation EC50)×100
(PA and PB are respectively in mixed agent to be had TI × PB of TI × PA+ single dose B of the theoretical toxicity index TTI=of mixed agent single dose AThe percentage composition of effect composition)
Theoretical toxicity index TTI × 100 of the actual toxicity index ATI/ mixed agent of co-toxicity coefficient CTC=mixed agent
Synergistic effect judgement: CTC >=120, have synergistic effect; 80 < CTC < 120 are summation action; CTC≤80, for short of moneyAnti-effect.
(4) results and analysis: as shown in table 1～table 10.
The toxicity test result of table 15 kind of medicament to falsesour cherry root carninomatosis bacterium
Table 2 Zhongshengmycin and the toxicity test result of Shen piperazine mycin different proportion mixture to falsesour cherry root carninomatosis bacterium
From table 1～2, Zhongshengmycin, Shen piperazine mycin and mixture preparation thereof, have good inhibition to falsesour cherry root carninomatosis bacterium and doWith, when wherein the proportioning of Zhongshengmycin and Shen piperazine mycin is between 1:1～1:35, to all existing for the co-toxicity coefficient of examination pathogenMore than 120, show that these two kinds of bactericide have good synergistic function, especially proportioning in 1:5～1 under this proportioning:Between 25, synergistic effect is more remarkable.
The toxicity test result of table 35 kind of medicament to ginger plague germ
Table 4 Zhongshengmycin and the toxicity test result of Shen piperazine mycin different proportion mixture to ginger plague germ
From table 3～4, Zhongshengmycin, Shen piperazine mycin and mixture preparation thereof, have good inhibitory action to ginger plague germ,When wherein the proportioning of Zhongshengmycin and Shen piperazine mycin is between 1:1～1:35, to supplying the co-toxicity coefficient of examination pathogen all 120Above, show that these two kinds of bactericide have good synergistic function under this proportioning, especially proportioning is at 1:10～1:30Between synergistic effect more remarkable.
The toxicity test result of table 55 kind of medicament to armillariella root rot of apple bacterium
Table 6 Zhongshengmycin and the toxicity test result of Shen piperazine mycin different proportion mixture to armillariella root rot of apple bacterium
From table 5～6, Zhongshengmycin, Shen piperazine mycin and mixture preparation thereof, have good inhibition to armillariella root rot of apple bacterium and doWith, when wherein the proportioning of Zhongshengmycin and Shen piperazine mycin is between 1:10～1:20, to all existing for the co-toxicity coefficient of examination pathogenMore than 120, show that these two kinds of bactericide have good synergistic function, especially proportioning in 1:10～1 under this proportioning:Between 15, synergistic effect is more remarkable.
The toxicity test result of table 75 kind of medicament to Alternaria mali
Table 8 Zhongshengmycin and the toxicity test result of Shen piperazine mycin different proportion mixture to Alternaria mali
From table 7～8, Zhongshengmycin, Shen piperazine mycin and mixture preparation thereof, have well and press down Alternaria maliMake use, when wherein the proportioning of Zhongshengmycin and Shen piperazine mycin is between 1:1～1:15, to the co-toxicity coefficient for examination pathogenSubstantially all, more than 120, show that these two kinds of bactericide have synergistic function under this proportioning, especially proportioning 1:5～Between 1:15, synergistic effect is comparatively remarkable.
The toxicity test result of table 95 kind of medicament to Rhizoctonia solani Kuhn
Table 10 Zhongshengmycin and the toxicity test result of Shen piperazine mycin different proportion mixture to Rhizoctonia solani Kuhn
From table 9～10, Zhongshengmycin, Shen piperazine mycin and mixture preparation thereof, have good inhibition to Rhizoctonia solani KuhnEffect, when wherein the proportioning of Zhongshengmycin and Shen piperazine mycin is between 1:5～1:20, to equal for the co-toxicity coefficient of examination pathogenMore than 120, show that these two kinds of bactericide have good synergistic function under this proportioning, and synergistic effect is more remarkable.
Embodiment 15% Zhongshengmycin Shen piperazine mycin wettable powder
Component (each components based on weight percentage) composed as follows:
Preparation method is: above-mentioned each active component, various auxiliary agent and filler are fully mixed in proportion, and mixture is through ultra-finePulverizer is pulverized, and makes 5% Zhongshengmycin Shen piperazine mycin wettable powder.
This embodiment is applied to prevention and prevents and treats falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters early stage, different denseDegree is watered draws furrow irrigation root, and every 15 days once, connects and execute 3 times; Prevent and treat rice sheath blight disease, alternaria leaf spot of apple, at the beginning of entering morbidityPhase, the control of variable concentrations foliar spray, once, the season of growth is used 2～3 times in medication in every 10～15 days. Under prevention effect is shown inTable 11 (note: in table 11～table 16,3% described Zhongshengmycin WP, the composition of its auxiliary material is with embodiment 1, and described 1%Shen piperazine mycin SC, the composition of its auxiliary material is with embodiment 2. Each extension rate in table, solid to wettable powder, water dispersible granules etc.Body formulation, refers to quality multiple, for other formulation, refers to volume multiple).
Table 11 Zhongshengmycin preparations different from Shen piperazine mycin control falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, rice sheath blight disease,The field effect of alternaria leaf spot of apple
Can find out by table 11, after Zhongshengmycin and Shen piperazine mycin mixture synergistic effect obvious, to the prevention effect of five kinds of diseasesSignificantly better than single dose, and effective ingredient consumption obviously reduces.
Embodiment 225% Zhongshengmycin Shen piperazine mycin suspending agent
Component (each components based on weight percentage) composed as follows:
Preparation method is: above-mentioned each active component, various auxiliary agent and water are fully mixed in proportion, drop into sand mill, grindBe milled to particle diameter 5 μ m, 25% Zhongshengmycin Shen piperazine mycin suspending agent.
This embodiment is applied to prevention and prevents and treats falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters early stage, different denseDegree is watered draws furrow irrigation root, and every 15 days once, connects and execute 3 times; Prevent and treat rice sheath blight disease, alternaria leaf spot of apple, at the beginning of entering morbidityPhase, the control of variable concentrations foliar spray, once, the season of growth is used 2～3 times in medication in every 10～15 days. Under prevention effect is shown inTable 12.
Table 12 Zhongshengmycin preparations different from Shen piperazine mycin control falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, rice sheath blight disease,The field effect of alternaria leaf spot of apple
Can find out by table 12, after Zhongshengmycin and Shen piperazine mycin mixture synergistic effect obvious, to the prevention effect of five kinds of diseasesSignificantly better than single dose, and effective ingredient consumption obviously reduces.
Embodiment 311% Zhongshengmycin Shen piperazine mycin missible oil
Component (each components based on weight percentage) composed as follows:
Preparation method is: above-mentioned each active component, various auxiliary agent and water are fully mixed in proportion, add in mixing kettle, stirUniform 11% Zhongshengmycin Shen piperazine mycin missible oil.
This embodiment is applied to prevention and prevents and treats falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters early stage, different denseDegree is watered draws furrow irrigation root, and every 15 days once, connects and execute 3 times; Prevent and treat rice sheath blight disease, alternaria leaf spot of apple, at the beginning of entering morbidityPhase, the control of variable concentrations foliar spray, once, the season of growth is used 2～3 times in medication in every 10～15 days. Under prevention effect is shown inTable 13.
Table 13 Zhongshengmycin preparations different from Shen piperazine mycin control falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, rice sheath blight disease,The field effect of alternaria leaf spot of apple
Can find out by table 13, after Zhongshengmycin and Shen piperazine mycin mixture synergistic effect obvious, to the prevention effect of five kinds of diseasesSignificantly better than single dose, and effective ingredient consumption obviously reduces.
Embodiment 4:16% Zhongshengmycin Shen piperazine mycin aqueous emulsion
Component (each components based on weight percentage) composed as follows:
Preparation method is: above-mentioned each active component, various auxiliary agent are fully mixed in proportion, make to be dissolved into all and oil phase, at a high speedUnder stirring, oil phase is mixed with water, obtains 16% Zhongshengmycin Shen piperazine mycin aqueous emulsion.
This embodiment is applied to prevention and prevents and treats falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters early stage, different denseDegree is watered draws furrow irrigation root, and every 15 days once, connects and execute 3 times; Prevent and treat rice sheath blight disease, alternaria leaf spot of apple, at the beginning of entering morbidityPhase, the control of variable concentrations foliar spray, once, the season of growth is used 2～3 times in medication in every 10～15 days. Under prevention effect is shown inTable 14.
Table 14 Zhongshengmycin preparations different from Shen piperazine mycin control falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, rice sheath blight disease,The field effect of alternaria leaf spot of apple
Can find out by table 14, after Zhongshengmycin and Shen piperazine mycin mixture synergistic effect obvious, to the prevention effect of five kinds of diseasesSignificantly better than single dose, and effective ingredient consumption obviously reduces.
Embodiment 5:6% Zhongshengmycin Shen piperazine mycin water dispersible granules
Component (each components based on weight percentage) composed as follows:
Preparation method is: above-mentioned each active component, Negel formaldehyde condensation products, wetting agent, disintegrant, filler are pressedRatio is fully mixed, and is crushed to particle diameter 5 μ m through airslide disintegrating mill, obtains female powder, after female powder is fully mixed with sodium lignin sulfonateGranulation, dry, sieve, thereby make 6% Zhongshengmycin Shen piperazine mycin water-dispersible granules.
This embodiment is applied to prevention and prevents and treats falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters early stage, different denseDegree is watered draws furrow irrigation root, and every 15 days once, connects and execute 3 times; Prevent and treat rice sheath blight disease, alternaria leaf spot of apple, at the beginning of entering morbidityPhase, the control of variable concentrations foliar spray, once, the season of growth is used 2～3 times in medication in every 10～15 days. Under prevention effect is shown inTable 15.
Table 15 Zhongshengmycin preparations different from Shen piperazine mycin control falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, rice sheath blight disease,The field effect of alternaria leaf spot of apple
Can find out by table 15, after Zhongshengmycin and Shen piperazine mycin mixture synergistic effect obvious, to the prevention effect of five kinds of diseasesSignificantly better than single dose, and effective ingredient consumption obviously reduces.
Embodiment 6:26% Zhongshengmycin Shen piperazine mycin microemulsion
Component (each components based on weight percentage) composed as follows:
Preparation method is: above-mentioned each active component, various auxiliary agent are fully mixed in proportion, make to be dissolved into all and oil phase, at a high speedUnder stirring, oil phase is mixed with water, obtains 26% Zhongshengmycin Shen piperazine mycin microemulsion.
This embodiment is applied to prevention and prevents and treats falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, enters early stage, different denseDegree is watered draws furrow irrigation root, and every 15 days once, connects and execute 3 times; Prevent and treat rice sheath blight disease, alternaria leaf spot of apple, at the beginning of entering morbidityPhase, the control of variable concentrations foliar spray, once, the season of growth is used 2～3 times in medication in every 10～15 days. Under prevention effect is shown inTable 16.
Table 16 Zhongshengmycin preparations different from Shen piperazine mycin control falsesour cherry root carninomatosis, bacterial wilt of ginger, armillariella root rot of apple, rice sheath blight disease,The field effect of alternaria leaf spot of apple
Can find out by table 16, after Zhongshengmycin and Shen piperazine mycin mixture synergistic effect obvious, to the prevention effect of five kinds of diseasesSignificantly better than single dose, and effective ingredient consumption obviously reduces.
Claims (8)
1. containing a biological antibiotic composition for Zhongshengmycin, it is characterized in that: active ingredient is Zhongshengmycin and Shen piperazine mycin,The weight ratio of Zhongshengmycin and Shen piperazine mycin is 1:1～35.
2. the biological antibiotic composition containing Zhongshengmycin according to claim 1, is characterized in that: described containing ZhongshengmycinBiological antibiotic composition formed by the component of following weight portion: Zhongshengmycin 1%～80%, Shen piperazine mycin 1%～80%, more thanAmount is used and acceptable auxiliary material for allowing in Pesticide Science.
3. the biological antibiotic composition containing Zhongshengmycin according to claim 1, is characterized in that: described containing ZhongshengmycinThe formulation of biological antibiotic composition be wettable powder, suspending agent, missible oil, aqueous emulsion, water dispersible granules or microemulsion.
4. according to the bactericidal composition containing Zhongshengmycin described in any one in claim 1～3, it is characterized in that: in described containingThe formulation of the biological antibiotic composition of raw rhzomorph is wettable powder, is made up of: middle raw bacterium the component of following percetage by weightElement 1%, Shen piperazine mycin 4%, Negel formaldehyde condensation products 3%, sodium lignin sulfonate 4%, lauryl sodium sulfate 4%,White Carbon black 20%, surplus is kaolin.
5. according to the bactericidal composition containing Zhongshengmycin described in any one in claim 1～3, it is characterized in that: in described containingThe formulation of the biological antibiotic composition of raw rhzomorph is suspending agent, is made up of the component of following percetage by weight: Zhongshengmycin 1%,Shen piperazine mycin 24%, Negel formaldehyde condensation products 6%, castor oil polyoxyethylene ether 4%, AEO 6%,Sodium methylene bis-naphthalene sulfonate 5%, xanthans 3%, glycerine 5%, surplus is water.
6. according to the bactericidal composition containing Zhongshengmycin described in any one in claim 1～3, it is characterized in that: in described containingThe formulation of the biological antibiotic composition of raw rhzomorph is missible oil, is made up of the component of following percetage by weight: Zhongshengmycin 1%,Shen piperazine mycin 10%, cyclohexane 27%, agricultural emulsifier NO．600 4%, castor oil polyethylene glycol condensation 7%, glycerine 5%, surplus is twoToluene.
7. according to the bactericidal composition containing Zhongshengmycin described in any one in claim 1～3, it is characterized in that: in described containingThe formulation of the biological antibiotic composition of raw rhzomorph is aqueous emulsion or water dispersible granules or microemulsion;
In the time that formulation is aqueous emulsion, formed by the component of following percetage by weight: Zhongshengmycin 1%, Shen piperazine mycin 15%,Cyclohexane 22%, agricultural emulsifier NO．600 4%, castor oil polyethylene glycol condensation 4%, Negel formaldehyde condensation products 6%, excess water;
In the time that formulation is water dispersible granules, formed by the component of following percetage by weight: Zhongshengmycin 1%, Shen piperazine mycin 5%,Negel formaldehyde condensation products 6%, sodium lignin sulfonate 5%, sodium methylene bis-naphthalene sulfonate 3%, ammonium sulfate 4%, White Carbon black30%, surplus is calcium carbonate;
In the time that formulation is microemulsion, formed by the component of following percetage by weight: Zhongshengmycin 1%, Shen piperazine mycin 25%,Cyclohexane 17%, agricultural emulsifier NO．600 4%, castor oil polyethylene glycol condensation 6%, isopropyl alcohol 17%, surplus is water; Described cyclohexaneFor solvent; Described agricultural emulsifier NO．600 is emulsifying agent; The condensation of described castor oil polyethylene glycol is emulsifying agent; Described isopropyl alcohol is solvent.
8. in claim 1～7, the biological antibiotic composition containing Zhongshengmycin described in any one is being prevented and treated fruit tree, vegetables, cropOn disease in application; Described disease be selected from fruit root knot disease, bacterial wilt of ginger, root rot, rice sheath blight disease, downy mildew of garpe,Alternaria leaf spot of apple; In the time that control fruit root knot is sick, the two weight ratio of Zhongshengmycin and Shen piperazine mycin is 1:1～35; ?Control is when bacterial wilt of ginger, and the two weight ratio of Zhongshengmycin and Shen piperazine mycin is 1:1～35; In the time preventing and treating root rot, ZhongshengmycinWith the two weight ratio of Shen piperazine mycin be 1:10～20; In control when alternaria leaf spot of apple, Zhongshengmycin and Shen piperazine mycin the twoWeight ratio be 1:1～15; In the time preventing and treating rice sheath blight disease, the two weight ratio of Zhongshengmycin and Shen piperazine mycin is 1:5～20.
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| CN110731337B (en) * | 2019-10-28 | 2021-04-20 | 浙江省桐庐汇丰生物科技有限公司 | Composition for preventing and treating plant diseases and application thereof |
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| CN111700074A (en) * | 2020-07-08 | 2020-09-25 | 潍坊中创生物科技有限公司 | Preparation method and application of nematophagous fungus chlamydospore wettable powder |
| CN114982770A (en) * | 2022-06-16 | 2022-09-02 | 江西省农业科学院植物保护研究所 | Composition for preventing and treating sesame wilt and application thereof |
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| CN102812956A (en) * | 2012-09-18 | 2012-12-12 | 北京燕化永乐农药有限公司 | Bactericidal composition |
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| CN101171931A (en) * | 2007-12-06 | 2008-05-07 | 张少武 | Zhongshengmycin wettable powder and preparation method thereof |
| CN102812956A (en) * | 2012-09-18 | 2012-12-12 | 北京燕化永乐农药有限公司 | Bactericidal composition |
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