CN101669486A - Benzolkresoxim-methyl containing bactericide composition - Google Patents

Benzolkresoxim-methyl containing bactericide composition Download PDF

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CN101669486A
CN101669486A CN 200910206541 CN200910206541A CN101669486A CN 101669486 A CN101669486 A CN 101669486A CN 200910206541 CN200910206541 CN 200910206541 CN 200910206541 A CN200910206541 A CN 200910206541A CN 101669486 A CN101669486 A CN 101669486A
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kresoxim
mold
thiazol
composition
methyl
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CN101669486B (en
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建 孔
张承来
曹明章
毕湘黔
郭东岳
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深圳诺普信农化股份有限公司
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Abstract

The invention discloses a bactericide composition. The bactericide composition contains benzolkresoxim-methyl and benziothiazolinone, wherein, a weight ratio of the benzolkresoxim-methyl to the benziothiazolinone is 50:1-1:50, the composition also contains 1-80% of active constituents by total weight and acceptable auxiliary constituents allowed for use in pesticides. The benzolkresoxim-methyl andthe benziothiazolinone have obvious synergic action after being mixed at a certain ratio, and can be used for preventing cereal, melon and fruit or vegetable crops from disease.

Description

一种含苯醚菌酯的杀菌组合物 One kind of benzene fungicidal composition of kresoxim

技术领域 FIELD

本发明涉及一种杀菌组合物,尤其是一种含苯醚菌酯的二元复配组合物。 The present invention relates to a fungicidal composition, in particular a benzene binary composite composition of kresoxim.

背景技术 Background technique

苯醚菌酯(研发代号:ZJ0712),化学名称为:2-[2-(2,5-二甲基苯氧基)-苯亚甲基]-3-甲氧基丙烯酸甲酯,是一种甲氧基丙烯酸酯类内吸杀菌剂,具有保护和治疗作用。 Kresoxim-methyl phenyl (Research Code: ZJ0712), chemical name: 2- [2- (2,5-dimethylphenoxy) - benzylidene] -3-methoxy-acrylate, a species fungicide strobilurin, with protective and therapeutic effect. 该药剂对卵菌、子囊菌、担子菌、半知菌引起的多种植物病害都有很高的杀菌活性,尤其是对白粉病、霜霉病、炭疽病等病害有良好的生物活性,可用于瓜类、果树、蔬菜、小麦、烟草、花卉等作物的病害防治。 The agent of oomycetes, ascomycetes, basidiomycetes, imperfect various plant diseases caused by bacteria have a high bactericidal activity, especially those with good biological activity powdery mildew, downy mildew, anthracnose diseases, available disease control in melons, fruit trees, vegetables, wheat, tobacco, flowers and other crops.

在作物病害的防治过程中,最常见的问题是病菌抗药性的产生。 In the process of prevention and treatment of crop diseases, the most common problem is to produce drug-resistant bacteria. 苯醚菌酯是新研发的药剂,目前的生产和使用成本均较高,难以大面积推广应用。 Kresoxim-phenyl is a new drug developed, using current production costs are high and it is difficult in large scale. 此外如果长期单一施用苯醚菌酯单剂容易诱使病害产生严重的抗性。 Further, if long-term administration of a single a single dose phenyl kresoxim readily induce resistance to serious disease.

复配增效好的杀菌剂组合使用,可大幅提高实际防治效果,降低农药的使用量和成本,从而避免病菌抗药性产生或延缓病菌抗性的产生速度。 Synergism good bactericides used in combination, can significantly increase the actual control effect, reduce the amount of pesticides and cost, so as to avoid or delay the development of resistance of bacteria to produce bacteria resistant speed.

发明内容 SUMMARY

本发明的目的是提供一种高效、安全、环保的杀菌组合物以满足农业生产的需要,组合物具有增效作用,能提高防效、降低成本并有益于延缓病菌抗药性的产生。 Object of the present invention is to provide an efficient, safe, environmentally friendly germicidal composition to meet the needs of agricultural production, a composition having a synergistic effect, can improve the control efficiency, reduce costs and delay the beneficial bacteria resistance.

本发明的技术方案如下: Aspect of the present invention is as follows:

一种杀菌组合物,其特征是活性成分为苯醚菌酯和噻霉酮,其中苯醚菌酯与噻霉酮的重量比为50∶1~1∶50,优选比例为20∶1~1∶20。 A fungicidal composition, characterized in that the active ingredient is phenyl and thiophene mold kresoxim-one, wherein the weight ratio of benzene and thiophene kresoxim one mold is 50:1 to 1:50, preferably a ratio of ~ 1 20:1 :20. 组合物中两种活性组分的重量百分含量为1~80%,优选为1~30%。 Composition, the weight percentage of the two active ingredients is 1 to 80%, preferably from 1 to 30%.

所述噻霉酮(benziothiazolinone)为一种有机杂环类杀菌剂,作用机理是破坏病菌细胞核结构,干扰病菌细胞的新陈代谢,使其生理紊乱,最终导致死亡。 The mold thiazol-one (benziothiazolinone) is an organic heterocyclic fungicides, bacteria destruction mechanism is nuclear architecture, metabolism interfering bacteria cells, so physiological disorder, eventually leading to death. 具有高效、广谱、低毒、绿色环保等特点,对细菌、真菌性病害具有预防和治疗作用。 With high efficiency, broad-spectrum, low toxicity, environmental protection and other characteristics of bacterial, fungal diseases have preventive and therapeutic effects. 主要用于防治黄瓜霜霉病、梨黑星病、苹果疮痂病、柑橘炭疽病、葡萄黑痘病等多种病害。 Mainly used for prevention and treatment of various diseases of cucumber downy mildew, scab, apple scab, anthracnose of citrus, grape anthracnose disease.

本发明的农药组合可以用已知的方法制备成适合农业使用的任意一种剂型,比较好的剂型为可湿性粉剂、悬浮剂、水分散粒剂、水乳剂和微乳剂。 Pesticide compositions of the present invention can be prepared by known methods into any dosage form suitable for agricultural use, the dosage form is better wettable powders, suspensions, water dispersible granules, aqueous emulsions and microemulsions. 本发明的组合物使用的助剂包括溶剂、分散剂、乳化剂、防冻剂、增稠剂等及其它有益于有效成分在制剂中稳定和药效发挥的已知物质,都是农药制剂中常用或允许使用的各种成分,并无特别限定,具体成分和用量根据配方要求通过简单试验确定。 Adjuvant composition used in the present invention include solvents, dispersants, emulsifiers, antifreeze agents, thickeners, and other beneficial and stabilizing the active ingredient in the formulation efficacy is known to play materials are commonly used in pesticide formulations or allow the use of the various ingredients is not particularly limited, and specific ingredients and amounts determined by simple experiment according to the recipe requirements.

本发明杀菌组合物可应用于防治卵菌、子囊菌、担子菌、半知菌等病原真菌引起的多种植物病害,适用于防治禾谷类、各种瓜果、蔬菜的作物病害,如瓜类霜霉病、苹果斑点落叶病、油菜菌核病、辣椒炭疽病、香蕉叶斑病、梨黑星病、柑橘炭疽病、芒果炭疽病,特别是防治作物霜霉病、炭疽病等病害。 The fungicidal compositions according to the invention is applicable to prevention Oomycetes, Ascomycetes, Basidiomycetes, microorganisms imperfect various plant diseases caused by pathogenic fungi, suitable for controlling cereal, various fruits, vegetables crop diseases, such as melons downy mildew, apple leaf spot, Sclerotinia, anthracnose pepper, banana leaf spot, scab, anthracnose of citrus, mango anthracnose, especially the prevention and treatment of crop downy mildew, anthracnose and other diseases.

本发明的有益效果: Advantageous effects of the invention:

1、混配具有明显的增效作用,提高了防治效果。 1, mixed with obvious synergies, improve the control effect.

2、本发明所用的苯醚菌酯是甲氧基丙烯酸酯类化合物,噻霉酮是有机杂环类化合物,两者作用机制不同,作用位点不同,无交互抗药性,相互混配病菌不易产生抗药性。 2, phenyl kresoxim used in the present invention is a strobilurin compound, an organic ketone thiazol mold heterocyclic compounds, two different mechanisms of action, different site of action, no cross-resistance, bacteria easily mixed with each other resistance.

3、使用成本降低。 3, the use of cost reduction. 药效提高后复配药剂中有效成分的用量比单剂明显减少,从而降低了成本和减轻了对环境的污染。 Compound medicament active ingredients with improved efficacy after single dose ratio significantly reduced, thereby reducing the cost and reduce the pollution of the environment.

具体实施方式 Detailed ways

为了使本发明的目的、技术方案及优点更加清楚明白,本发明用以下具体实施例进行说明,但本发明绝非限于这些例子。 To make the objectives, technical solutions and advantages of the present invention will become more apparent, the present invention is illustrated by the following specific examples, but the present invention is by no means limited to these examples. 以下所述仅为本发明较好的实施例,仅仅用以解释本发明,并不能因此而理解为对本发明专利范围的限制。 The following descriptions are merely preferred embodiments of the present invention, only intended to illustrate the present invention, and can not therefore be understood as limiting the scope of the present invention. 应当指出的是,凡在本发明的精神和原则之内所做的任何修改、等同替换和改进等,均应包含在本发明的保护范围之内。 It should be noted that any modifications made within the spirit and principle of the present invention, equivalent substitutions and improvements should be included within the scope of the present invention. 因此,本发明专利的保护范围应以所附权利要求为准。 Therefore, the protection scope of the present invention should be subject to the appended claims.

1、悬浮剂实施例 1, suspensions Example

按配方比例,将农药活性组分苯醚菌酯和噻霉酮、表面活性剂、增稠剂、成膜剂、载体或填料和部分水混合后,经过高速剪切,湿法砂磨,制成半成品,分析后补水混合均匀过滤即获得成品。 Press the formula ratio of the pesticide active ingredient kresoxim-methyl phenyl ketone, and thiophene mold, a surfactant, a thickener, film former carrier water were mixed, and part or fillers, after the high shear, wet sanding, Ltd. into a semi-finished product, the post-analysis filtered to obtain a uniform mixing replenishment products.

实施例1:11%苯醚菌酯·噻霉酮悬浮剂 Example 1: 11% phenyl-thiazol kresoxim one mold SC

98%苯醚菌酯(活性组分) 1.02g Kresoxim-methyl phenyl 98% (active ingredient) 1.02 g of

95%噻霉酮(活性组分) 10.53g 95% mycophenolate thiophene-one (active ingredient) 10.53 g

木质素磺酸钠(分散剂) 1g Sodium lignosulfonate (dispersant) 1g

硅酸铝镁(增稠剂) 1g Magnesium aluminum silicate (thickener) 1g

乙二醇(抗冻剂) 5g Ethylene glycol (anti-freezing agent) 5g

白炭黑(增稠剂) 2g Silica (thickener) 2g

膨润土(增稠剂) 2g Bentonite (thickener) 2g

水 77.45g Water 77.45g

实施例2:21%苯醚菌酯·噻霉酮悬浮剂 Example 2: 21% phenyl-thiazol kresoxim one mold SC

98%苯醚菌酯(活性组分) 20.41g Kresoxim-methyl phenyl 98% (active ingredient) 20.41g

95%噻霉酮(活性组分) 1.05g 95% mycophenolate thiophene-one (active ingredient) 1.05 g of

木质素磺酸钠(分散剂) 1g Sodium lignosulfonate (dispersant) 1g

白炭黑(增稠剂) 2g Silica (thickener) 2g

膨润土(增稠剂) 2g Bentonite (thickener) 2g

硅酸铝镁(增稠剂) 1g Magnesium aluminum silicate (thickener) 1g

乙二醇(抗冻剂) 5g Ethylene glycol (anti-freezing agent) 5g

水 67.54g Water 67.54g

2、可湿性粉剂实施例 2, wettable powders Example

将农药活性组分苯醚菌酯和噻霉酮、助剂、填料按比例混合经气流粉碎后再混合制得可湿性粉剂。 The pesticide active ingredient kresoxim-methyl phenyl ketone, and thiophene mold, aids, fillers mixed in proportions and mixed to obtain wettable powder was then jet milling. 主要设备:混合机、气流粉碎机。 Major equipment: mixer, jet mill.

实施例3:51%苯醚菌酯·噻霉酮可湿性粉剂 Example 3: 51% phenyl-thiazol kresoxim one mold WP

98%苯醚菌酯(活性组分) 51.02g Kresoxim-methyl phenyl 98% (active ingredient) 51.02g

95%噻霉酮(活性组分) 1.05g 95% mycophenolate thiophene-one (active ingredient) 1.05 g of

木质素磺酸钠(分散剂) 5g Sodium lignosulfonate (dispersant) 5g

茶枯粉(分散剂) 5g Dry tea powder (dispersing agent) 5g

拉开粉(润湿剂) 1g Nekal (wetting agent) 1g

白炭黑(填料) 1g Silica (filler) 1g

凹凸棒土 35.93g Attapulgite 35.93g

实施例4:80%苯醚菌酯·噻霉酮可湿性粉剂 Example 4: 80% of the phenyl-thiazol kresoxim one mold WP

98%苯醚菌酯(活性组分) 51.02g Kresoxim-methyl phenyl 98% (active ingredient) 51.02g

95%噻霉酮(活性组分) 31.58g 95% mycophenolate thiophene-one (active ingredient) 31.58 g

木质素磺酸钠(分散剂) 2g Sodium lignosulfonate (dispersant) 2g

十二烷基硫酸钠(润湿剂) 5g Sodium lauryl sulfate (a wetting agent) 5g

硅藻土 10.4g Diatomite 10.4g

3、微乳剂、水乳剂实施例 3, microemulsions, aqueous emulsion Example

按各组分配比,将苯醚菌酯溶解在溶剂中,加入表面活性剂,混合均匀制得油相;将噻霉酮和水混合制得水相;再将油相加入水相中或水相加入油相中,边加边搅拌,制得微乳剂或水乳剂。 According to the distribution ratio for each group, the phenyl kresoxim dissolved in a solvent, adding a surfactant, mixing uniformly to prepare an oil phase; thiazol the mold ketone and water were prepared by mixing an aqueous phase; then the aqueous phase or oil phase was added water phase was added to the oil phase, with stirring, to prepare an aqueous emulsion or microemulsion.

实施例5:1%苯醚菌酯·噻霉酮微乳剂 Example 5: 1% phenyl-thiazol kresoxim one mold microemulsions

98%苯醚菌酯(活性组分) 0.51g Kresoxim-methyl phenyl 98% (active ingredient) 0.51 g of

95%噻霉酮(活性组分) 0.53g 95% mycophenolate thiophene-one (active ingredient) 0.53 g of

二甲苯(溶剂) 5g Xylene (solvent) 5g

丙二醇(溶剂) 5g Propylene glycol (solvent) 5g

蓖麻油聚氧乙烯醚(乳化剂) 8g Polyoxyethylene castor oil (emulsifier) ​​8g

十二烷基苯磺酸钙(乳化剂) 3g Calcium dodecylbenzenesulfonate (emulsifier) ​​3g

水 76.96g Water 76.96g

实施例6:10.5%苯醚菌酯·噻霉酮水乳剂 Example 6: 10.5% kresoxim-thiazol phenyl ketone mold water emulsion

98%苯醚菌酯(活性组分) 0.51g Kresoxim-methyl phenyl 98% (active ingredient) 0.51 g of

95%噻霉酮(活性组分) 10.53g 95% mycophenolate thiophene-one (active ingredient) 10.53 g

DMF(溶剂) 5g DMF (solvent) 5g

二甲苯(溶剂) 10g Xylene (solvent) 10g

甘油(抗冻剂) 5g Glycerol (antifreeze) 5g

苯乙基酚聚氧乙烯醚(乳化剂) 10g Phenethyl phenol polyoxyethylene ether (emulsifier) ​​10g

十二烷基苯磺酸钙(乳化剂) 5g Calcium dodecylbenzenesulfonate (emulsifier) ​​5g

水 44.8g Water 44.8g

4、水分散性粒剂实施例 4, water dispersible granules Example

将农药活性组分苯醚菌酯和噻霉酮、助剂和填料按比例混合,经气流粉碎,然后加15-20%的水,经捏合、造粒、干燥、筛分制得水分散粒剂产品。 The pesticide active ingredient kresoxim-methyl phenyl ketone, and thiophene mold, additives and fillers mixed in proportion, by jet milling, then adding 15-20% water, kneaded, granulated, dried and sieved to obtain water dispersible granules agent product. 主要设备:混合机、气流粉碎机、捏合机、挤压造粒机、干燥设备(烘房或流化床)。 Major equipment: a mixer, a jet mill, a kneader, extrusion granulator, drying equipment (drying room or fluidized bed).

实施例7:51%苯醚菌酯·噻霉酮水分散粒剂 Example 7: 51% phenyl-thiazol mold kresoxim one water dispersible granules

98%苯醚菌酯(活性组分) 1.02g Kresoxim-methyl phenyl 98% (active ingredient) 1.02 g of

95%噻霉酮(活性组分) 52.63g 95% mycophenolate thiophene-one (active ingredient) 52.63g

木质素磺酸钠(分散剂) 5g Sodium lignosulfonate (dispersant) 5g

甲基萘磺酸钠甲醛缩合物(分散剂) 15g Methyl naphthalene sulfonate formaldehyde condensate (dispersant) 15g

十二烷基硫酸钠(润湿剂) 5g Sodium lauryl sulfate (a wetting agent) 5g

海藻酸钠(填料) 5g Sodium alginate (filler) 5g

陶土 16.35g Clay 16.35g

实施例8:30%苯醚菌酯·噻霉酮水分散粒剂 Example 8: 30% of the phenyl-thiazol mold kresoxim one water dispersible granules

98%苯醚菌酯(活性组分) 5.10g Kresoxim-methyl phenyl 98% (active ingredient) 5.10 g

95%噻霉酮(活性组分) 26.32g 95% mycophenolate thiophene-one (active ingredient) 26.32g

木质素磺酸钠(分散剂) 5g Sodium lignosulfonate (dispersant) 5g

茶枯粉(分散剂) 2g Dry tea powder (dispersing agent) 2g

十二烷基硫酸钠(润湿剂) 2g Sodium lauryl sulfate (a wetting agent) 2g

膨润土 59.58g Bentonite 59.58g

应用实施例: Application Example:

1、苯醚菌酯与噻霉酮复配对黄瓜霜霉病菌盆栽试验测定 1, kresoxim phenyl ketone thiazol complex mold pot test measures pairing cubensis

试验对象:黄瓜霜霉病菌(Pseudoperonospora cubensis) Subjects: cucumber downy mildew (Pseudoperonospora cubensis)

试验方法:参照《中华人民共和国农业行业标准NY/T 1156.11-2008》盆栽法。 Test method: refer to "People's Republic of China agricultural industry standards NY / T 1156.11-2008" pot method. 选取生长势基本一致的两片真叶期黄瓜苗作为实验材料,每个处理选用5盆黄瓜苗,编号供试。 Select growth potential substantially uniform two true leaves of cucumber plants as experimental materials, the choice of 5 pots per treatment cucumber seedlings, the test number. 取采自田间的黄瓜霜霉病叶片,用毛笔将病菌用无菌水洗下,配制成浓度为3×10 5个孢子囊/毫升的孢子囊悬浮液,喷雾接种于黄瓜苗叶面上(每处理10毫升)。 Pseudoperonospora cubensis collected from the field to take the blade, with a brush germs with sterile water, formulated to a concentration of 3 × 10 5 sporangia / ml of sporangia suspension, spray-inoculated on the leaf surface of the cucumber plants (each processing 10 ml). 接种后保湿培养,24h后进行药剂处理,试验药剂参见表1。 Moisturizing culture after inoculation for 24h after drug treatment, the test drug in Table 1. 用Potter喷雾塔在50PSI压力下喷雾,每盆5mL。 With a Potter spray tower at 50PSI pressure spray, pot 5mL. 以喷施等量清水的处理为空白对照。 To process the same amount of water for spraying blank. 喷雾后将黄瓜苗放入人工气候室中培养。 After spraying cucumber seedlings into a climatic chamber culture. 7d后按照黄瓜霜霉病的发病分级标准调查全部叶片并记载统计、计算病情指数和防治效果。 After 7d investigation according to the standard classification cucumber downy mildew incidence and all the blades according to statistics, the disease index and control effect is calculated.

将防治效果换算成几率值(y),药液浓度(μg/ml)转换成对数值(x),以最小二乘法计算毒力方程和抑制中浓度EC 50 ,依孙云沛法计算药剂的毒力指数及共毒系数(CTC)。 The control effect in terms of the probability value (y), solution concentration (μg / ml) converting a logarithmic value (x), the least square method to calculate equation toxicity and inhibition concentration EC 50, calculated by Sun Yunpei agent virulence Method index and toxicity coefficient (CTC).

实测毒力指数(ATI)=(标准药剂EC 50 /供试药剂EC 50 )×100 Found toxicity index (ATI) = (standard agents EC 50 / Sample Agent EC 50) × 100

理论毒力指数(TTI)=A药剂毒力指数×混剂中A的百分含量+B药剂毒力指数×混剂中B的百分含量 Percentage of theoretical toxicity index (TTI) = A × germicide index in mixture A + B in × germicide index mixture percentage of agent B

共毒系数(CTC)=[混剂实测毒力指数(ATI)/混剂理论毒力指数(TTI)]×100 Toxicity coefficient (CTC) = [Mixtures Found toxicity index (ATI) / agent mixture Theoretical toxicity index (TTI)] × 100

CTC≤80,组合物表现为拮抗作用;80<CTC<120,组合物表现为相加作用;CTC≥120,组合物表现为增效作用。 CTC≤80, compositions were antagonistic; 80 <CTC <120, the composition exhibits an additive effect; CTC≥120, the composition showed synergistic action.

测定结果见表1,苯醚菌酯与噻霉酮在配比50∶1~1∶50之间,对黄瓜霜霉病菌具有增效作用,尤其在20∶1~1∶20之间,增效作用更明显,共毒系数在200以上。 The measurement results are shown in Table 1, between 1 to 1:50 50:1 phenyl kresoxim-one ratio with thiazol mold, bacteria have a synergistic effect on cucumber downy mildew, in particular between 20:1 to 1:20, by more significantly pleiotropic effects, co-toxicity coefficients of 200 or more.

表1苯醚菌酯与噻霉酮复配对黄瓜霜霉病盆栽试验测定结果 Table 1 and kresoxim-thiazol phenyl ketone Complex as the measurement result mildew Cucumber Downy Mildew pot experiment

处理 deal with EC 50 (μg/ml) EC 50 (μg / ml) ATI ATI TTI TTI 共毒系数(CTC) Toxicity coefficient (CTC) 苯醚菌酯 Phenyl kresoxim 1.85 1.85 100 100 - - - - 噻霉酮 Thiazol one mold 4.28 4.28 43.22 43.22 - - - - 苯醚菌酯1∶噻霉酮50 Kresoxim-methyl phenyl ketone 1: thiazol mold 50 3.01 3.01 61.46 61.46 44.34 44.34 138.62 138.62 苯醚菌酯1∶噻霉酮30 Kresoxim-methyl phenyl ketone 1: thiazol mold 30 2.72 2.72 68.01 68.01 45.06 45.06 150.96 150.96

苯醚菌酯1∶噻霉酮20 Kresoxim-methyl phenyl ketone 1: thiazol mold 20 1.81 1.81 102.21 102.21 45.93 45.93 222.54 222.54 苯醚菌酯1∶噻霉酮10 Kresoxim-methyl phenyl ketone 1: thiazol mold 10 1.62 1.62 114.20 114.20 48.39 48.39 236.01 236.01 苯醚菌酯1∶噻霉酮1 Kresoxim-methyl phenyl ketone 1: thiazol mold 1 1.03 1.03 179.61 179.61 71.61 71.61 250.81 250.81 苯醚菌酯10∶噻霉酮1 Kresoxim-methyl phenyl ketone 10: thiazol mold 1 0.82 0.82 225.61 225.61 94.84 94.84 237.89 237.89 苯醚菌酯20∶噻霉酮1 Kresoxim-methyl phenyl ketone 20: thiazol mold 1 0.85 0.85 217.65 217.65 97.30 97.30 223.69 223.69 苯醚菌酯30∶噻霉酮1 Kresoxim-methyl phenyl ketone 30: thiazol mold 1 1.16 1.16 159.48 159.48 98.17 98.17 162.46 162.46 苯醚菌酯50∶噻霉酮1 Kresoxim-methyl phenyl ketone 50: thiazol mold 1 1.35 1.35 137.04 137.04 98.89 98.89 138.58 138.58

2、苯醚菌酯与噻霉酮复配对芒果炭疽病室内毒力测定。 2, phenyl and kresoxim thiazol one mold counterpart multiplex assay Colletotrichum gloeosporioides Toxicity.

试验对象:芒果炭疽病菌(Colletotrichum gloeosporioides),采自田间并经三次分离纯化。 Subjects: Colletotrichum gloeosporioides (Colletotrichum gloeosporioides), collected from three separate field purified and dried.

试验方法:参照《中华人民共和国农业行业标准NY/T 1156.2-2006》菌丝生长速率法。 Test method: refer to "People's Republic of China agricultural industry standards NY / T 1156.2-2006" mycelial growth rate. 将芒果炭疽病菌用PDA培养基培养,待菌落刚长满培养皿时,用内径为7mm的打孔器从边缘打孔,打成的菌丝块用作接种体。 When the culture medium by Colletotrichum gloeosporioides PDA, just until the colonies covered dish with an inner diameter of 7mm from the edge of the puncher punching, labeled as block mycelium inoculum. 分别取配好的药液5mL与定量的75mL灭菌培养基混合均匀,制成4个含药平板,以等量无菌水与培养基混合为对照。 They were taken with a good chemical and quantitative 75mL 5mL sterilized medium uniformly mixed to prepare a drug-containing plates 4, an equivalent amount of sterile water mixed with a culture medium as a control. 将菌丝块倒置接于平板中央,并于28℃培养箱内培养。 The mycelium inverted block connected to the central plate, and cultured in an incubator at 28 ℃. 7d后,用十字交叉法测量菌落直径,计算各处理菌落直径净生长量、菌丝生长抑制率。 After 7D, measured by colony diameter cross method, calculate the net amount of each growth treatment the colony diameter, mycelial growth inhibition.

菌落净生长量(mm)=测量菌落直径-7 Net colony growth (mm) = colony diameter measured -7

菌丝生长抑制率(%)=[(对照组菌落净生长量-处理组菌落净生长量)/对照组菌落净生长量]×100 Mycelial growth inhibition rate (%) = [(amount of net growth of colonies in control group - amount of net growth of colonies treated group) / Net growth of colonies in the control group] × 100

将菌丝生长抑制率换算成几率值(y),药液浓度(μg/ml)转换成对数值(x),以最小二乘法计算毒力方程和抑制中浓度EC 50 ,依孙云沛法计算药剂的毒力指数及共毒系数(CTC)。 The mycelial growth inhibition rate in terms of the probability value (y), solution concentration (μg / ml) converting a logarithmic value (x), the least square method to calculate equation toxicity and inhibition concentration EC 50, the drug calculation method by Sun Yunpei virulence index and toxicity coefficient (CTC). 公式同应用实例1。 Formula 1 with application examples.

结果(表2)显示,苯醚菌酯与噻霉酮在配比50∶1~1∶50之间,对芒果炭疽病菌具有增效作用,尤其在20∶1~1∶20之间,增效作用更明显,共毒系数在200以上。 The results (Table 2) show, phenyl and thiophene mold kresoxim-one ratio between 50:1 to 1:50, have a synergistic effect on Colletotrichum gloeosporioides, in particular between 20:1 to 1:20, by more significantly pleiotropic effects, co-toxicity coefficients of 200 or more.

表2苯醚菌酯与噻霉酮复配对芒果炭疽病菌室内毒力测定结果 Table 2 phenyl ketone kresoxim mold pair complex thiazol Colletotrichum gloeosporioides Bioassay Results

处理 deal with EC 50 (μg/ml) EC 50 (μg / ml) ATI ATI TTI TTI 共毒系数(CTC) Toxicity coefficient (CTC) 苯醚菌酯 Phenyl kresoxim 2.07 2.07 100 100 - - - - 噻霉酮 Thiazol one mold 4.81 4.81 43.04 43.04 - - - - 苯醚菌酯1∶噻霉酮50 Kresoxim-methyl phenyl ketone 1: thiazol mold 50 3.64 3.64 56.87 56.87 44.15 44.15 128.80 128.80 苯醚菌酯1∶噻霉酮30 Kresoxim-methyl phenyl ketone 1: thiazol mold 30 2.79 2.79 74.19 74.19 44.87 44.87 165.34 165.34 苯醚菌酯1∶噻霉酮20 Kresoxim-methyl phenyl ketone 1: thiazol mold 20 2.21 2.21 93.67 93.67 45.75 45.75 204.74 204.74 苯醚菌酯1∶噻霉酮10 Kresoxim-methyl phenyl ketone 1: thiazol mold 10 1.81 1.81 114.36 114.36 48.21 48.21 237.20 237.20 苯醚菌酯1∶噻霉酮1 Kresoxim-methyl phenyl ketone 1: thiazol mold 1 1.15 1.15 180.00 180.00 71.52 71.52 251.69 251.69 苯醚菌酯10∶噻霉酮1 Kresoxim-methyl phenyl ketone 10: thiazol mold 1 0.91 0.91 227.47 227.47 94.82 94.82 239.90 239.90 苯醚菌酯20∶噻霉酮1 Kresoxim-methyl phenyl ketone 20: thiazol mold 1 1.01 1.01 204.95 204.95 97.29 97.29 210.66 210.66 苯醚菌酯30∶噻霉酮1 Kresoxim-methyl phenyl ketone 30: thiazol mold 1 1.22 1.22 169.67 169.67 98.16 98.16 172.85 172.85 苯醚菌酯50∶噻霉酮1 Kresoxim-methyl phenyl ketone 50: thiazol mold 1 1.49 1.49 138.93 138.93 98.88 98.88 140.50 140.50

3、苯醚菌酯与噻霉酮复配对防治黄瓜霜霉病的田间药效试验。 3, and phenyl kresoxim thiazol one mold Complex as against cucumber downy mildew field efficacy trials.

(1)实验处理:本试验各药剂处理及用量按照表3,对照药剂分别是市售农药10%苯醚菌酯悬浮剂和1.5%噻霉酮水乳剂,另设清水对照。 (1) Experimental process: The test process and the amount of each agent in Table 3, pesticide control agents commercially available are 10% phenyl kresoxim 1.5% suspending agent and one mold thiazol water emulsions, water separate control.

(2)试验方法:试验选择在历年霜霉病比较严重的黄瓜种植地块中进行,各处理的黄瓜长势、农药及肥水管理等条件基本一致。 (2) Test Method: Test calendar selection downy mildew in cucumber cultivation serious plots, each process of growing cucumber, pesticides and fertilizer management conditions are basically the same. 于霜霉病发病初期,每隔7天喷施一次,共喷施3次。 In the early onset of downy mildew, spraying once every 7 days, a total of three times spraying. 每处理四个小区重复,小区面积20平方米。 Each treatment was repeated four cells, a cell area of ​​20 square meters. 在药前和第3次施药后7天调查霜霉病发病情况。 Before and after the third drug spraying 7 days investigating the incidence of downy mildew. 每小区5点取样,每点调查2株,每株调查全部叶片。 Each cell 5 sampling points, each point of investigation 2, all leaves per survey. 计算各处理的病情指数及防效。 Calculation disease index and control effect of each treatment.

(3)试验结果如下: (3) Test results were as follows:

由田间药效试验结果(表3)可以看出:苯醚菌酯或噻霉酮单剂对黄瓜霜霉病的防效分别为81.71%、81.52%,而本发明实施例杀菌组合物在防效方面有显著提高,最低防效为88.27%,最高达到了96.44%,特别是苯醚菌酯与噻霉酮重量份数比为20∶1~1∶20(实施例1、2、4、5、6、8)时,防效提升尤为明显,对黄瓜霜霉病防效达到了93%以上,因此,从田间药效试验来看,苯醚菌酯与噻霉酮复配对黄瓜霜霉病防治效果好,具有协同增效作用。 The field experiments (Table 3) can be seen: phenyl or thiazol kresoxim one single dose of anti-mold effect on cucumber downy mildew was 81.71%, 81.52% respectively, the sterilizing composition and the anti embodiment of the present invention significantly increased efficiency in the minimum control efficiency of 88.27%, 96.44% up to the, in particular kresoxim-methyl and phenyl ketone thiazol mold parts by weight ratio 20:1 to 1:20 (Examples 1,2,4, when 5,6,8), especially to enhance the control effect on cucumber downy mildew control effect of more than 93%, therefore, from the point of view field efficacy trials, phenyl kresoxim-one complex with mildew cucumber thiazol pairing Peronospora disease prevention and treatment effect, has a synergistic effect.

表3苯醚菌酯与噻霉酮复配对黄瓜霜霉病的田间试验结果 Table 3 and kresoxim-methyl phenyl ketone thiazol mold pair complex field results Pseudoperonospora cubensis

供试药剂 Sample Agent 用药量(g·ai/ha) Dosage (g · ai / ha) 药前病指 Prodrug disease index 第3次药后7天病指 7 days after the 3rd disease agent refers 防效(%) Control effect (%) 10%苯醚菌酯SC 10% phenyl kresoxim SC 15 15 1.5 1.5 12.1 12.1 81.71 81.71 1.5%噻霉酮WP 1.5% WP thiazol one mold 25 25 1.3 1.3 10.6 10.6 81.52 81.52 实施例1 Example 1 14 14 1.6 1.6 4.1 4.1 94.19 94.19 实施例2 Example 2 12 12 1.9 1.9 5.2 5.2 93.80 93.80 实施例3 Example 3 18 18 1.7 1.7 8.7 8.7 88.40 88.40 实施例4 Example 4 13 13 1.6 1.6 3.1 3.1 95.61 95.61 实施例5 Example 5 15 15 1.4 1.4 2.2 2.2 96.44 96.44 实施例6 Example 6 20 20 1.3 1.3 4.0 4.0 93.02 93.02 实施例7 Example 7 23 twenty three 1.7 1.7 8.8 8.8 88.27 88.27 实施例8 Example 8 19 19 1.6 1.6 3.5 3.5 95.04 95.04 Ck Ck - - 1.6 1.6 70.6 70.6 - -

*注:g·ai/ha表示每公顷的有效成分用量 * Note: g · ai / ha represents the amount of active ingredient per hectare

Claims (7)

1、一种杀菌组合物,其特征在于:其活性组分为苯醚菌酯与噻霉酮。 1. A fungicidal composition comprising: an active component which phenyl and thiophene kresoxim one mold.
2、根据权利要求1,其特征在于:组合物中苯醚菌酯与噻霉酮的重量比为50∶1~1∶50。 2, according to claim 1, wherein: the composition and kresoxim phenyl ketone mold thiazol weight ratio of 50 to 1:50.
3、根据权利要求2,其特征在于:组合物中苯醚菌酯与噻霉酮的重量比为20∶1~1∶20。 3, according to claim 2, wherein: the composition and kresoxim-thiazol phenyl ketone mold 20:1 to 1:20 weight ratio.
4、根据权利要求1至3任一项所述的杀菌组合物,其特征在于:组合物中两种活性组分总重量百分含量为1~80%。 4, a germicidal composition according to any one of claims 1-3, characterized in that: the total weight of the composition, the percentage of the two active ingredients is 1 to 80%.
5、根据权利要求4所述的杀菌组合物,其特征在于:组合物中两种活性组分总重量百分含量为1~30%。 5. The germicidal composition as claimed in claim 4, wherein: the two compositions by weight of the total percentage of active ingredient is 1 to 30%.
6、根据权利要求4所述的杀菌组合物,其特征在于:组合物剂型是可湿性粉剂、悬浮剂、水分散粒剂、微乳剂、水乳剂。 6. The germicidal composition according to claim 4, wherein: the composition is a dosage form of wettable powders, suspensions, water dispersible granules, microemulsions, emulsion water.
7、根据权利要求1至3任一项所述的杀菌组合物,其特征在于:可用于防治禾谷类、果树及蔬菜发生的多种病害。 7, a germicidal composition according to any one of claims 1-3, characterized in that: can be used to control various diseases of cereals, fruit trees and vegetables occurs.
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CN106561675A (en) * 2016-10-20 2017-04-19 陕西西大华特科技实业有限公司 Sterilization composition comprising benziothiazolinone and coumoxystrobin
CN106561676A (en) * 2016-11-10 2017-04-19 江苏辉丰农化股份有限公司 Bactericide composition containing benzisothiazolones and coumoxystrobin
CN106577721A (en) * 2016-11-11 2017-04-26 江苏辉丰农化股份有限公司 Bactericidal composition containing benzisothiazolinone and moroxydine hydrochloride
CN106359405A (en) * 2016-11-11 2017-02-01 江苏辉丰农化股份有限公司 Bactericide composition containing benzisothiazolone and ethylicin
CN106818784A (en) * 2017-01-20 2017-06-13 广东中迅农科股份有限公司 A kind of bactericidal composition containing 2-cyano-3-amino-3-phenylancryic acetate and benziothiazolinone
CN106942240A (en) * 2017-02-28 2017-07-14 江苏辉丰农化股份有限公司 A kind of microbicide compositions comprising emodin derivates and BIT
CN107006490A (en) * 2017-05-24 2017-08-04 广东中迅农科股份有限公司 A kind of bactericidal composition containing probenazole, benziothiazolinone

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