CN103493818A - Sterilization composition containing metconazole and JS399-19 - Google Patents
Sterilization composition containing metconazole and JS399-19 Download PDFInfo
- Publication number
- CN103493818A CN103493818A CN201310434876.2A CN201310434876A CN103493818A CN 103493818 A CN103493818 A CN 103493818A CN 201310434876 A CN201310434876 A CN 201310434876A CN 103493818 A CN103493818 A CN 103493818A
- Authority
- CN
- China
- Prior art keywords
- wheat
- metconazole
- amino
- cyano
- phenylancryic acetate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a sterilization composition which contains metconazole and JS399-19 (the chemical name of which is 2-cyan-3-amino-3-ethyl-phenylacrylate and the test number of which is 'JS399-19'), which serve as effective active components. When the weight ratio of the metconazole to the JS399-19 is (1-40):(40-1), a synergistic interaction effect can be achieved on suppression of the growth of gibberella saubinetii. The composition has a wide bacteriocidal spectrum and is mainly used for preventing and treating the wheat scab, the powdery mildew and the rust disease, and a preventing and treating effect is outstanding; the preventing effect is stabilized over 80 percent; the sterilization composition has the advantages of high fast-acting property and high persistence; furthermore, the yield of wheat can be increased, and the quality of the wheat can be improved. A working mechanism of the sterilization composition is novel; the number of working sites is large; the sterilization composition can treat the drug resistance of gibberella saubinetii to carbendazim as well as the powdery mildew to triadimefon; the generation and the development of the drug resistance of pathogenic bacteria to a triazole fungicide are delayed.
Description
Technical field
A kind of bactericidal composition that contains metconazole and 2-cyano-3-amino-3-phenylancryic acetate, belong to two kinds of fungicide mixtures of novel, efficient, the low toxicity that can be used for preventing and treating the multiple fungal disease of wheat crops.
Background technology
Wheat is the important cereal crops of China.Head blight, powdery mildew and rust are the important diseases of harm Wheat Production, and the Long-term Effect the yield and quality of China's wheat, causes economic loss.China mainly adopts the triazole bactericidal agents such as triazolone (triadimefon), Tebuconazole, Difenoconazole to carry out prior to seeding seed treatment or spray the benzimidazole germicides such as triazolone and carbendazim in vegetative period being prevented and treated at present.But, use for a long time these single-acting site bactericide, have been found that Powdery Mildew and gibberellic hypha have produced pesticide resistance to triadimefon and carbendazim respectively, make the continuous and effective control of China's wheat diseases be faced with severe challenge.The present invention is directed to the present situation of China's wheat diseases chemical control, by using the bactericidal composition of the different mechanisms of action, be intended to reach the reduction pesticide dosage, improve control efficiency and administer drug-fast target.
2-cyano-3-amino-3-phenylancryic acetate (2-cyano group-3-amino-3-ethyl phenylacrylate, tested number: be JS399-19) syntheticly in 1998 by Jiangsu base, National Research and Development Center for Pesticides a kind of sickle-like bacteria to be had to the active compound of higher specialization, particularly the Fusarium graminearum mycelial growth had to strong inhibition activity.This product calendar year 2001 enters the field demonstration test, demonstrates the bright market prospects of preventing and treating wheat scab.Obtain at present Chinese invention patent, within 2005, completed agricultural chemicals and register temporarily, become the new type bactericide with independent intellectual property right.Have research to point out, 2-cyano-3-amino-3-phenylancryic acetate is expected to become a kind of alternative medicament of administering wheat scab, the area of especially carbendazim being developed immunity to drugs.But desk research shows, the medicament that gibberellic hypha is high at this type of medicament is selected to depress very easily to develop immunity to drugs, and has certain resistance risk.Therefore in the situation that at present disease-resistant variety lack, in order to delay or to overcome the drug-fast formation and development of pathogen, avoid single use 2-cyano-3-amino-3-phenylancryic acetate continuously, should or mix use with the bactericide rotation of other types.
Metconazole is the triazole bactericidal agent of a kind of efficient, wide spectrum, interior absorption, has protection, treatment, eradicant action, thereby is widely used in the control by fungus-caused multiple diseases such as sac fungi, basidiomycetes.Metconazole is one of effective agent of preventing and treating at present wheat scab, and its mechanism of action is mainly the inhibition synthetic to the disease fungus ergosterol, and it can not only reduce the severity of wheat head blight, can reduce the content of toxin in seed simultaneously.
Pesticide resistance and susceptibility Mechanism Study based on fusarium graminearum to 2-cyano-3-amino-3-phenylancryic acetate, find that it is relevant with biological membrane permeability.Therefore, the present invention selects and disturbs metconazole and the 2-cyano-3-amino-3-phenylancryic acetate of membrane permeability to carry out the synergistic formula screening.
Summary of the invention
Technical problem the object of the invention be to provide a kind of can the integrated control wheat diseases and there is the bactericidal composition of synergistic function.The bactericidal composition of invention is that drug effect is good, quick-acting good, synergy is remarkable, fungicidal spectrum is wide, with low cost, two kinds of bacteriocide formulas delaying drug-fast generation, use safety and be convenient to promote the use of.
A kind of bactericidal composition that contains metconazole and 2-cyano-3-amino-3-phenylancryic acetate of technical scheme, it is characterized in that mixing to use by two kinds of different bactericide of the mechanism of action enlarging antimicrobial spectrum, and the destruction by the saturating property of metconazole cell membrane is improved the effect of 2-cyano-3-amino-3-phenylancryic acetate and the synergistic effect of performance group compound utilizes the synergistic effect of bactericidal composition to reduce the bactericide consumption and improve the control efficiency to disease.Purposes of the present invention is mainly to prevent and treat wheat scab, double other fungal diseases of controlling on wheat.
The beneficial effect inventor is by operation technique, mechanism of action and the pesticide resistance research of the generation to plant disease, control medicament, for the invention provides the theory and technology basis.The present invention, by utilizing new type bactericide 2-cyano-3-amino-3-phenylancryic acetate and metconazole to carry out compound screening, its objective is the acquisition synergistic formula, reduces pesticide dosage and production cost, raising preventive effect, administers pesticide resistance.
The present invention is through indoor and field control effectiveness test, and its result shows, metconazole and 2-cyano-3-amino-3-phenylancryic acetate physicochemical property are held mutually, use safety, and drug effect is remarkable.Compare and there is following advantage with other agricultural chemicals:
1. bactericidal composition of the present invention belongs to the pesticide combination of efficient, low toxicity, low-residual, environmentally compatible.
2. this formula belongs to composite insecticide, has compatibility, has improved bactericidal activity, has reduced the dosage of single bactericide, has saved the agricultural chemicals use cost.
3. this prescription medicament can reduce the resistance risk level of pathogen to chemical agent, is conducive to the maintenance of pathogen susceptibility, can delay germ simultaneously pesticide resistance is appearred in single dose in formula.
4. the bactericidal effect of this formula is remarkable, and energy effectively preventing gibberella zeaze petch of wheat and barley, powdery mildew and rust, administer the pesticide resistance of pathogen to triazolone and carbendazim, has reduced disease crops are produced to causing harm of causing, and has improved the yield and quality of agricultural product.
Specific embodiments and effect
(1) formula indoor screening
With to the responsive (MBC of carbendazim
s) and pesticide resistance (MBC
r) fusarium graminearum for the test target, the composition of indoor measurement metconazole and 2-cyano-3-amino-3-phenylancryic acetate different proportion is to the virulence of target and make mutually coefficient of synergism.Its method adopts metconazole and the former medicine of 2-cyano-3-amino-3-phenylancryic acetate to be dissolved in respectively in varing proportions solvent, then sneaks into culture matrix, measures the responsive (MBC of wheat scab carbendazim
s) and resistance (MBC
r) the inhibition activity of bacterial strain mycelial growth, calculate EC
50value and coefficient of synergism (the results are shown in Table 1).
According to table 1 result, can find out, when the proportioning of metconazole and 2-cyano-3-amino-3-phenylancryic acetate is 40～1:1～40, to the EC of the fusarium graminearum of carbendazim sensitivity and resistance
50be 0.0267～0.2934 μ g/ml, coefficient of synergism is 1.60～2.22, has all reached the effect of synergy.
Table 1 metconazole 2-cyano-3-amino-3-phenylancryic acetate formula indoor the selection result
In table, " leaf " represents metconazole; " cyanogen " represents 2-cyano-3-amino-3-phenylancryic acetate.
(2) field operation technique and drug effect thereof
Metconazole and 2-cyano-3-amino-3-phenylancryic acetate mixture are prevented and treated wheat scab
Embodiment 1. field trials
By metconazole and 2-cyano-3-amino-3-phenylancryic acetate respectively in the ratio composition of active ingredient 5:1,1:5,1:10 and 1:20, add auxiliary agent and carrier, wherein 5:1 and 1:10 proportioning are processed into wetting powder (WP), 1:5 and 1:20 proportioning and are processed into suspending agent (SC) fungicide preparation, number consecutively composition 1,2,3,4, carry out field trial.
Experimental field be arranged in farm, lake, Baima, Huaian, Jiangsu and Yancheng City Xin Yang farm that gibberellic hypha has developed immunity to drugs to carbendazim.Wheat breed is respectively the Huaihe River wheat No. 22, Huaihe River wheat No. 33.In November, 2012 premature rice sowing, field management is carried out routinely, does not use other agricultural chemicals.On May 2nd, 2013 is to adopt AGROLEX SPRAYER JACTOHD profit agriculture 16L knapsack sprayer to carry out dispenser for the first time at the wheat flowering initial stage, according to carrying out dispenser for the second time weather forecast May 9 (Early filling stage).Each community area is 50 square metres, 4 repetitions, and water consumption is 50Kg/ mu, not dispenser of blank.Spraying chemicals treatment comprises: single dose 60g/L metconazole SC, 25% 2-cyano-3-amino-3-phenylancryic acetate SC and metconazole 2-cyano-3-amino-3-phenylancryic acetate built agent 1,2,3,4 (formulation rate is in Table 2,3).On May 26th, 2013 is carried out state of an illness investigation, and control efficiency is in Table 2,3.
Field test results shows, metconazole and 2-cyano-3-amino-3-phenylancryic acetate all have excellent prevention and control capability preventing and treating aspect wheat scab, control efficiency is all more than 80%, after both are composite, sprayed, not only effectively reduce the incidence of disease, disease index, improve control efficiency, also reduced dosage (table 2 and 3).
Farm, lake, table 2 Baima-metconazole 2-cyano-3-amino-3-phenylancryic acetate is prevented and treated the effect of wheat scab
Table 3 Xin Yang farm-metconazole 2-cyano-3-amino-3-phenylancryic acetate is prevented and treated the effect of wheat scab
Investigation simultaneously finds, composition 1,2,3 and 4, in the experimental plot on farm, lake, Baima, also reaches respectively 95.6%, 91.5%, 81.4% and 67.2% to the preventive effect of wheat powdery mildew, illustrates that said composition can doublely control wheat powdery mildew.
Embodiment 2. seed treatment tests
Test is located at plant protection institute of Agricultural University Of Nanjing solarium, uses disposal plastic cup, and matrix is turfy soil.The punching of plastic cup bottom, be placed on pallet, the middle water that adds in pallet.Every glass of 20 strains.Adopt prior to seeding the bactericidal composition 1,2,3 of above-mentioned metconazole 2-cyano-3-amino-3-phenylancryic acetate, the method for 4 four kind of formula seed dressing to carry out seed treatment, establish effective composition 5g ai/100Kg seed, 10g ai/100Kg seed, 20g ai/100Kg seed and four kinds of processing of blank.Process respectively the 100g wheat seed by treatment dosage, make seed evenly attach liquid, sow after drying 24h.Every processing 4 basins.After medication, make regular check on, until medication is after 2 weeks.Direct paired observation method and mensuration are taked in investigation.
Result of the test shows, after the medicament seed treatment, has effectively reduced the microbial bud-rot of head blight and seedling withered, makes emergence rate improve 5.85%～10.05%.The wheat seeding leaf look dark green of simultaneously also observing processing, and width of blade increases, and plant is sturdy.Test show to use this bactericidal composition to carry out seed treatment not only can to prevent and treat bud-rot, seedling that head blight causes withered, improve the emergence rate of seed, also there is the effect in strong sprout.
Test is also found, while growing to 2 leaf 1 heart, wheat seeding is carried out to wheat powdery mildew and the inoculation of wheat leaf rust germ, at composition 1, almost can not fall ill on the wheat seeding of 20g ai/100Kg seed treatment, and preventive effect reaches 99.68%.Other combinations and dosage are processed also certain protection effect, and preventive effect all (the results are shown in Table 4) more than 80%.
The protection effect of table 4 metconazole tpn composition seed treatment to wheat powdery mildew, wheat rust
Claims (3)
1. a bactericidal composition that contains metconazole and 2-cyano-3-amino-3-phenylancryic acetate, is characterized in that, when the weight ratio of metconazole and 2-cyano-3-amino-3-phenylancryic acetate is 40～1:1～40, the inhibition of wheat scab bacteria growing had to synergistic effect.
2. based on claim 1, the bactericidal composition that contains metconazole and 2-cyano-3-amino-3-phenylancryic acetate has the bacteriostatic activity similar to wild sensitive strain to the carbendazim resistance fusarium graminearum.
3. based on claim 1 and 2, adopt the fungicide preparation of this bactericidal composition processing to carry out seed treatment, or be watered spraying at wheat growth stage and can prevent and treat wheat scab, powdery mildew, rust etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310434876.2A CN103493818A (en) | 2013-09-24 | 2013-09-24 | Sterilization composition containing metconazole and JS399-19 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310434876.2A CN103493818A (en) | 2013-09-24 | 2013-09-24 | Sterilization composition containing metconazole and JS399-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103493818A true CN103493818A (en) | 2014-01-08 |
Family
ID=49859163
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310434876.2A Pending CN103493818A (en) | 2013-09-24 | 2013-09-24 | Sterilization composition containing metconazole and JS399-19 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103493818A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719807A (en) * | 2016-11-30 | 2017-05-31 | 谢松芬 | The compound drug of wheat scab |
| CN106962363A (en) * | 2017-05-27 | 2017-07-21 | 南京农业大学 | A kind of collaboration decrement bactericidal composition containing metconazole |
| CN107006486A (en) * | 2017-05-25 | 2017-08-04 | 江苏徐淮地区淮阴农业科学研究所 | A kind of collaboration decrement composition pesticide of 2-cyano-3-amino-3-phenylancryic acetate-containing and its application |
| CN107094768A (en) * | 2017-06-15 | 2017-08-29 | 江苏徐淮地区淮阴农业科学研究所 | A kind of collaboration decrement composition pesticide containing Enestroburin |
| CN108841987A (en) * | 2018-07-05 | 2018-11-20 | 南京农业大学 | A kind of rapid detection method of Fusarium graminearum 2-cyano-3-amino-3-phenylancryic acetate resistant strain |
| CN109362727A (en) * | 2018-12-13 | 2019-02-22 | 安阳工学院 | A kind of wheat scab Biological agents and preparation method thereof |
| CN115677536A (en) * | 2022-10-28 | 2023-02-03 | 南京农业大学 | Synthetic method and application of cyantraniliprole and derivatives thereof |
| NL2031438B1 (en) | 2022-03-29 | 2023-10-20 | Huaiyin Inst Of Agricultural Sciences Of Xuhuai Region In Jiangsu | Synergistic decrement pesticide composition containing phenamacril and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101595890A (en) * | 2009-05-07 | 2009-12-09 | 深圳诺普信农化股份有限公司 | A kind of 2-cyano-3-amino-3-phenylancryic acetate and triazole type composition and application thereof |
-
2013
- 2013-09-24 CN CN201310434876.2A patent/CN103493818A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101595890A (en) * | 2009-05-07 | 2009-12-09 | 深圳诺普信农化股份有限公司 | A kind of 2-cyano-3-amino-3-phenylancryic acetate and triazole type composition and application thereof |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719807A (en) * | 2016-11-30 | 2017-05-31 | 谢松芬 | The compound drug of wheat scab |
| CN107006486A (en) * | 2017-05-25 | 2017-08-04 | 江苏徐淮地区淮阴农业科学研究所 | A kind of collaboration decrement composition pesticide of 2-cyano-3-amino-3-phenylancryic acetate-containing and its application |
| CN106962363A (en) * | 2017-05-27 | 2017-07-21 | 南京农业大学 | A kind of collaboration decrement bactericidal composition containing metconazole |
| CN107094768A (en) * | 2017-06-15 | 2017-08-29 | 江苏徐淮地区淮阴农业科学研究所 | A kind of collaboration decrement composition pesticide containing Enestroburin |
| CN108841987A (en) * | 2018-07-05 | 2018-11-20 | 南京农业大学 | A kind of rapid detection method of Fusarium graminearum 2-cyano-3-amino-3-phenylancryic acetate resistant strain |
| CN109362727A (en) * | 2018-12-13 | 2019-02-22 | 安阳工学院 | A kind of wheat scab Biological agents and preparation method thereof |
| NL2031438B1 (en) | 2022-03-29 | 2023-10-20 | Huaiyin Inst Of Agricultural Sciences Of Xuhuai Region In Jiangsu | Synergistic decrement pesticide composition containing phenamacril and application thereof |
| CN115677536A (en) * | 2022-10-28 | 2023-02-03 | 南京农业大学 | Synthetic method and application of cyantraniliprole and derivatives thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103493818A (en) | Sterilization composition containing metconazole and JS399-19 | |
| CN101700032B (en) | Bactericidal composition containing thiabendazole and application thereof | |
| CN101548677B (en) | Sterilizing compound of ethirimol and triazole | |
| CN102640748A (en) | Ultra-low-volume liquid containing prothioconazole | |
| CN106172421A (en) | A kind of containing metconazole and the bactericidal composition of Tebuconazole and application | |
| CN103444742A (en) | Sterilization composition containing metconazole and albendazole | |
| CN102669100B (en) | Oxine-copper and validamycin compound pesticide composition and preparation method and application thereof | |
| CN103329922B (en) | Compound sterilization composition, antiseptic and application of antiseptic | |
| CN102626080A (en) | Ultralow-volume liquid containing cyprodinil | |
| CN107494550A (en) | A kind of bactericidal composition containing fluorine azoles bacterium acyl azanol and 2-cyano-3-amino-3-phenylancryic acetate | |
| CN106857599A (en) | A kind of bactericidal composition containing pyraclostrobin and folpet and its application | |
| CN105901001A (en) | Bactericide composition containing metconazole and hymexazol | |
| CN105494359B (en) | A kind of composite bactericide for preventing and treating wheat scab and its application | |
| CN103081920B (en) | Sterilizing composition containing penconazole | |
| CN103444724A (en) | Bactericide composition containing metconazole and chlorothalonil | |
| CN102640757B (en) | Compound pesticide sterilization composition containing trifloxystrobin | |
| CN101779636B (en) | Bactericidal composite containing tetraconazole | |
| CN106070236A (en) | A kind of containing kind of bacterium azoles and the bactericidal composition of Tebuconazole and application | |
| CN107372548A (en) | Composition pesticide containing captan and fluorine azoles ring bacterium amine and application thereof | |
| CN103651396B (en) | Sterilization composition | |
| CN104351210A (en) | Preparation method of cottonseed bactericidal hybridization solution containing sodium dichloro cyanurate and hymexazol | |
| CN108782566A (en) | A kind of bactericidal composition of second containing pheno mycin and Osthole | |
| CN103503895A (en) | Antifungal composition containing pyrazole compound and triazole compound | |
| CN103651395B (en) | Sterilization composition | |
| CN101708004B (en) | Sterilizing composition containing active ester |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140108 |