CN103583525B - Pesticide composition containing phenazino-1-carboxylic acid - Google Patents
Pesticide composition containing phenazino-1-carboxylic acid Download PDFInfo
- Publication number
- CN103583525B CN103583525B CN201310570913.2A CN201310570913A CN103583525B CN 103583525 B CN103583525 B CN 103583525B CN 201310570913 A CN201310570913 A CN 201310570913A CN 103583525 B CN103583525 B CN 103583525B
- Authority
- CN
- China
- Prior art keywords
- amino
- piperazine mycin
- shen piperazine
- carboxylic acid
- phenazino
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention relates to pesticide composition containing phenazino-1-carboxylic acid, and belongs to the technical field of pesticides. The pesticide composition is characterized by comprising the phenazino-1-carboxylic acid, amino-oligosaccharin and brassinolide as effective components, wherein weight percentage of each effective component accounting for the total weight of the composition is as follows: 0.1%-10% of phenazino-1-carboxylic acid, 0.5%-20% of amino-oligosaccharin and 0.01%-2.0% of brassinolide; the effective components accounts for 0.61%-32% of the total weight of the composition; and a conventional method can be utilized to prepare each effective component as well as a solvent, a co-solvent and an auxiliary into a suspending agent, an aqueous emulsion or a microemulsion, and the pesticide composition is mainly used for treating and preventing one or more disease of vegetables, fruit trees, field crops and the like. According to the pesticide composition containing the phenazino-1-carboxylic acid, the phenazino-1-carboxylic acid is a biological bactericide, amino-oligosaccharin and brassinolide are natural products, the formed compound preparation agent not only has a bactericidal function, but also can effectively increase disease resistance and stress resistance of plants, and is safe and environment-friendly, and quality safety of agricultural products is guaranteed.
Description
Technical field:
The present invention relates to a kind of composition pesticide containing Shen piperazine mycin, belong to technical field of pesticide.
Background technology:
Shen piperazine mycin is a kind of biological bactericide secreted through biological culture by pseudomonas fluorescens M18, there is the dual-use function of wide spectrum Suppressing phytopathogens Promoting plant growth simultaneously, this biological bactericide has wide spectrum, efficiently feature, effectively can prevent and treat the fungal disease such as fusarium wilt, blight dis-ease, epidemic disease, banded sclerotial blight, false smut, rice blast, downy mildew, yellow rust, stalk break, head blight on the crops such as paddy rice, wheat, vegetables.
Amino-oligosaccharide (chitosan oligosaccharide) is the oligosaccharide that D-Glucosamine connects with β-1.4 glycosidic bond, after obtaining shitosan by chitin degrading, degraded obtains again, plant vivo gene can be excited, produce chitinase, dextranase, guarantor's element and the PR albumen etc. with resistant effect, and there is cell activation effect, the recovery of the plant that contributes to being injured, short root strong sprout, strengthen the resistance of crop, Promoting plant growth is grown.
Brassin lactones is the one that in steroidal compounds, biologically active is higher, and they are extensively present in plant corpus.In each stage of growth and development of plants, both can promote to nourish and grow, and can amphigamy be beneficial to again.Have take root, growth promoting effects, carry seedling, strong sprout, keep a full stand of seedings, yellowtop leaf turns green, promote fertilization, flower and fruit protecting, promotion Fruit are precocious, alleviate that disease is alleviated poisoning, coordinated nutrient balance, drought resisting is cold-resistant, reduce and make the multi-functional such as residue of pesticide in object, enhancing crop anti-adversity.
Chemical control is one of important measures of administering plant disease, but, due to long-term, single use, cause the reinforcement of its activity decrease and pathogen resistance, and the Long-Time Service of a large amount of chemical pesticide can cause the deterioration of ecotope and the generation of Quality Safety Problems of Agricultural Products.
Biological bactericide Shen piperazine mycin, Plant activator amino-oligosaccharide and natural source plant growth regulator brassin lactones carry out rationally composite by the present invention, effectively can avoid the problems referred to above.
Summary of the invention:
In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a kind of composition pesticide containing Shen piperazine mycin, Shen piperazine mycin in component is biological bactericide, and amino-oligosaccharide and brassin lactones are natural products, and the complex preparation formed is without environmental risk, not only there is sterilizing function, and effectively can also increase disease resistance and the resistance of plant, there is pre-disease prevention to occur and increase the function of crop yield, safety and environmental protection, can not environmental pollution be caused, ensure the quality safety of agricultural product.
The present invention realizes above-mentioned purpose by following technical solution.
A kind of composition pesticide containing Shen piperazine mycin provided by the present invention is active ingredient by Shen piperazine mycin, amino-oligosaccharide, brassin lactones, and the percentage by weight that wherein each active ingredient accounts for composition total weight is respectively:
Shen piperazine mycin: 0.1%-10%;
Amino-oligosaccharide: 0.5%-20%;
Brassin lactones: 0.01%-2.0%;
Described Shen piperazine mycin, its chemical molecular formula is C
13h
8n
2o
2, chemical name is: phenazine-1-carboxylic acid;
Described amino-oligosaccharide, its chemical molecular formula is formula is (C
6h
11nO
4)
n, wherein: n>=2, chemical name is: β-Isosorbide-5-Nitrae-oligosaccharides-gucosamine;
Described brassin lactones, its chemical name is: (22R, 23R, 24R)-2 α, 3 α, 22,23-tetrahydroxy-B-homogeneous phase-7-oxa--54-lumistane-6-ketone;
A kind of composition pesticide containing Shen piperazine mycin of the present invention is active ingredient by Shen piperazine mycin, amino-oligosaccharide, brassin lactones, and wherein each active ingredient accounts for that the percentage by weight of composition total weight is more excellent is respectively:
Shen piperazine mycin: 0.1%-5%;
Amino-oligosaccharide: 0.5%-10%;
Brassin lactones: 0.01%-1.0%;
Described active ingredient accounts for the 0.61%-32% of composition total weight;
Described composition, the method for available routine, with each active ingredient and solvent, cosolvent, auxiliary agent, makes suspending agent, aqueous emulsion, microemulsion;
Described solvent, cosolvent are: one or more the combination in toluene, dimethylbenzene, chloroform, paraffin, methyl alcohol, ethanol, propyl alcohol, butanols, phenmethylol, Benzophenone, methyl-sulfoxide, DMF, pyrrolidones, ethyl acetate, ethylene glycol bisthioglycolate ethylamino-, water;
Described auxiliary agent is: one or more the combination in alkyl sulfonates, phosphoric acid salt, lignin sulfonate, naphthalenesulfonate, phenolsulfonic acid salt, succinic acid Sulfonates, alkylphenol polyoxyethylene Sulfates, polystyrene-based phenol polyethenoxy ether Sulfates, polystyrene-based phenol polyethenoxy ethers, alkylphenol polyoxyethylene class, fatty alcohol polyethenoxy ether class, Span class, carboxyl alkyl cellulose class, tween, aluminium-magnesium silicate, xanthans.
Composition pesticide of the present invention is mainly used in treatment and the prevention of a kind of and multiple diseases such as vegetables, fruit tree, field crop.
The present invention compared with prior art, has following beneficial effect:
1, composition pesticide bactericidal range of the present invention is wide, all has certain effectiveness to field crop fungal disease.
2, composition pesticide of the present invention not only has sterilizing function, and effectively can also increase disease resistance and the resistance of plant, has pre-disease prevention to occur and increases the function of crop yield.
3, the Shen piperazine mycin in composition pesticide component of the present invention is biological bactericide, amino-oligosaccharide and brassin lactones are natural products, the complex preparation formed without environmental risk, safety and environmental protection, can not environmental pollution be caused, the quality safety of agricultural product can be ensured.
Embodiment:
Below in conjunction with specific embodiment, the invention will be further described:
Embodiment one:
A composition pesticide containing Shen piperazine mycin, wherein the percentage by weight of each component is:
Shen piperazine mycin: 1%, amino-oligosaccharide: 1%, brassin lactones: 1%, dodecyl sodium sulfate: 6%, Tween 80: 2%, sodium lignin sulfonate: 8%, aluminium-magnesium silicate: 0.2%, carboxymethyl cellulose: 0.1%, defoamer: 0.1%, water: surplus.
Embodiment two:
A composition pesticide containing Shen piperazine mycin, wherein the percentage by weight of each component is:
Shen piperazine mycin: 5%, amino-oligosaccharide: 5%, brassin lactones: 0.5%, dodecyl sodium sulfate: 6%, Tween 80: 2%, sodium lignin sulfonate: 8%, aluminium-magnesium silicate: 0.2%, carboxymethyl cellulose: 0.1%, defoamer: 0.1%, water: surplus.
Embodiment three:
A composition pesticide containing Shen piperazine mycin, wherein the percentage by weight of each component is:
Shen piperazine mycin: 10%, amino-oligosaccharide: 5%, brassin lactones: 1%, dodecyl sodium sulfate: 6%, Tween 80: 2%, sodium lignin sulfonate: 8%, aluminium-magnesium silicate: 0.2%, carboxymethyl cellulose: 0.1%, defoamer: 0.1%, water: surplus.
Embodiment four:
A composition pesticide containing Shen piperazine mycin, wherein the percentage by weight of each component is:
Shen piperazine mycin: 10%, amino-oligosaccharide: 20%, brassin lactones: 2%, dodecyl sodium sulfate: 6%, Tween 80: 2%, sodium lignin sulfonate: 8%, aluminium-magnesium silicate: 0.2%, carboxymethyl cellulose: 0.1%, defoamer: 0.1%, water: surplus.
Embodiment five:
Indoor bioassay embodiment: the composite Toxicity Determination to multiple pathogen of Shen piperazine mycin, amino-oligosaccharide and brassin lactones
The test method of indoor biometrics: adopt growth rate method to measure medicament to the inhibitory action of pathogen mycelial growth.On the basis of preliminary experiment, by further test, determine that Shen piperazine mycin single dose is to each EC for examination pathogen
50.According to the formula of embodiment 1,2,3,4, preparation Shen piperazine mycin concentration is its EC
50band poison medium, make band poison dull and stereotyped, receive each on flat board for examination bacterium bacterium cake.Often process arranges 3 repetitions, arranges blank.25 DEG C of constant temperature culture, when blank mycelia grows to and accounts for dull and stereotyped 2/3, take right-angled intersection method to measure colony diameter.Calculate the fungistatic effect of each medicament to pathogen.Above-mentioned bioassay test former medicine used and reagent provide by Zhuhai Zhenlvse Technology Co., Ltd.
Indoor bioassay test data is in table 1:
Table 1 Shen piperazine mycin, amino-oligosaccharide, brassin lactones and different ratio thereof are to each fungistatic effect for examination bacterium
Analyze above-mentioned data, can find out that the valid density of Shen piperazine mycin is at its EC
50condition under, add a certain amount of amino-oligosaccharide and brassin lactones respectively, its synergistic effect is remarkable.
The 10.0% Shen inhibiting rate of this proportioning of piperazine mycin+20.0% amino-oligosaccharide+2.0% brassin lactones to Rhizoctonia solani Kuhn, fusarium graminearum, Ustilaginoidea virens and walnut pine root fungus reaches 80.11%, 78.56%, 85.88%, 76.56% respectively.The bacteriostasis rate of other proportioning compares Shen piperazine mycin single dose, also has lifting.Generally, the interpolation of amino-oligosaccharide and brassin lactones has synergistic effect, with regard to medicament itself, to crop safety, environmentally friendly.This composition pesticide is the favourable agents of the water prevention rice sheath blight, wheat scab, rice blast and walnut root rot etc.
Embodiment six:
The field control effectiveness test of the water prevention rice sheath blight
Test site: village of Jingzhou City, Hubei
Test totally 5 process, each process repeats for 3 times, and community randomized arrangement adopts back-pack electric compression sprayer, and the record dispenser same day and duration of test meteorological data are in table 2:
Table 2 rice sheath blight disease controlling duration of test meteorological data
Control time and number of times:
Investigation state of an illness radix before first time medicine, 7d, 14d after 7d, second time medicine after first time medicine, investigate disease index respectively, investigate 4 times altogether.
Investigation method:
According to rice leaf sheath and blade damage symptom grading, in units of strain, every community diagonal 5 sampling, often some investigation is connected 5 clumps, totally 25 clumps, records total strain number, diseased plant number and sick progression.
Grade scale (taking sword-like leave as first leaf):
0 grade: complete stool is anosis; 1 grade: quaterfoil and following leaf sheath thereof, blade morbidity; 3 grades: Three-blade and following leaf sheath thereof, blade morbidity; 5 grades: second blade and following leaf sheath thereof, blade morbidity; 7 grades: sword-like leave blade and following leaf sheath thereof, blade morbidity; 9 grades: complete stool falls ill, withered ahead of time.
Drug effect computational methods:
Disease index is calculated by formula (1):
Pharmacy control efficacy is calculated by formula (2):
Table 3 rice sheath blight disease controlling field test results
As shown in Table 3, the Be very effective of the proportioning combination water prevention rice sheath blight of embodiment 1,2,3,4, after second time medication, the preventive effect of each embodiment of 7d is respectively 76.74%, 81.40%, 83.72%, 77.91%.
After second time medication, the preventive effect of each embodiment of 14d is respectively 78.95%, 97.89%, 84.21%, 87.37%.Each embodiment drug effect, all higher than single dose, therefore, can think that this composition pesticide has good effect to the water prevention rice sheath blight.
Embodiment seven:
The field trial of wheat scab control
Test site: certain colleges and universities' Experimental Base
Test totally 5 process, often process and repeat for 3 times, community randomized arrangement, adopt back-pack electric compression sprayer, the meteorological data of the record dispenser same day and duration of test, specifically in table 4:
Meteorological data during table 4 wheat scab controlling experiment
Investigation method: 5 samplings are taked, often 20 strains in every community, after dispenser, 14d investigation, investigates each community disease index, calculates preventive effect.
Severity divides 5 grades
0 grade: anosis; I level: sick small ear accounts for less than 1/4 of whole small ear; II level: sick small ear accounts for the 1/4-1/2 of whole small ear; III level: sick small ear accounts for the 1/2-3/4 of whole small ear; IV level: sick small ear accounts for more than 3/4 of whole small ear.
Drug effect computational methods:
Disease index is calculated by formula (3):
Pharmacy control efficacy is calculated by formula (4):
Table 5 wheat scab prevents and treats field test results
Embodiment eight:
The field control effectiveness test of control rice green smut
Test site: certain colleges and universities' Experimental Base
Test totally 5 process, often process and repeat for 3 times, community randomized arrangement, adopt back-pack electric compression sprayer, the record dispenser same day and duration of test meteorological data are in table 6.
Meteorological data during table 6 rice green smut controlling experiment
Investigation method: 5 samplings are taked, often 20 strains in every community, after dispenser, 7d, 14d, 21d investigation, investigates each community disease index, calculates preventive effect.
False smut grade scale:
0 grade: anosis; 1 grade: every fringe has an infected seed; 2 grades: every fringe has 2 ~ 4 infected seeds; 3 grades: every fringe has 5 ~ 7 infected seeds; 4 grades: every fringe has 8 ~ 10 infected seeds; 5 grades: every fringe has more than 10 infected seeds.
Disease index is calculated by formula (5):
Pharmacy control efficacy is calculated by formula (6):
Table 7 rice green smut prevents and treats field test results
As shown in Table 7, the Be very effective of the proportioning combination control rice green smut of embodiment 1,2,3,4 four embodiments.Each proportioning preventive effect is significantly greater than contrast single dose, and its control efficiency is excellent.7d after first time medication, each embodiment, compared with single dose, has certain synergistic effect.7d, 14d after second time medication, the preventive effect of each embodiment, all more than 80%, is significantly higher than single dose preventive effect.Can determine thus, each embodiment is the composition pesticide of control rice green smut excellence.
Comprehensive above field experiment result several times, the present invention is with the composite composition pesticide of Shen piperazine mycin, amino-oligosaccharide, brassin lactones, very excellent preventive effect is had to fungal diseases such as rice sheath blight disease, wheat scab, rice green smuts, relative single dose, have ill curing the disease, anosis diseases prevention, increases the characteristic of crop resistance.In addition, three is environmentally friendly, to crop safety, also has certain help, have good economic benefit and social benefit to the volume increase of crops.
Claims (3)
1. containing the composition pesticide of Shen piperazine mycin, it is characterized in that by Shen piperazine mycin, amino-oligosaccharide, brassin lactones be active ingredient, the percentage by weight that wherein each active ingredient accounts for composition total weight is respectively:
Shen piperazine mycin: 0.1%-10%;
Amino-oligosaccharide: 0.5%-20%;
Brassin lactones: 0.01%-2.0%;
Described Shen piperazine mycin, its chemical molecular formula is C
13h
8n
2o
2, chemical name is: phenazine-1-carboxylic acid;
Described amino-oligosaccharide, its chemical molecular formula is formula is (C
6h
11nO
4)
n, wherein: n>=2, chemical name is: β-Isosorbide-5-Nitrae-oligosaccharides-gucosamine;
Described brassin lactones, its chemical name is: (22R, 23R, 24R)-2 α, 3 α, 22,23-tetrahydroxy-B-homogeneous phase-7-oxa--54-lumistane-6-ketone.
2. a kind of composition pesticide containing Shen piperazine mycin according to claim 1, is characterized in that each active ingredient accounts for that the percentage by weight of composition total weight is more excellent is respectively:
Shen piperazine mycin: 0.1%-5%;
Amino-oligosaccharide: 0.5%-10%;
Brassin lactones: 0.01%-1.0%.
3. a kind of composition pesticide containing Shen piperazine mycin according to claim 1, is characterized in that described composition, by the method for routine, with each active ingredient and solvent, cosolvent, auxiliary agent, makes aqueous emulsion, microemulsion;
Described solvent, cosolvent are: one or more the combination in toluene, dimethylbenzene, chloroform, paraffin, methyl alcohol, ethanol, propyl alcohol, butanols, phenmethylol, Benzophenone, methyl-sulfoxide, DMF, pyrrolidones, ethyl acetate, ethylene glycol bisthioglycolate ethylamino-, water;
Described auxiliary agent is: one or more the combination in alkyl sulfonates, phosphoric acid salt, lignin sulfonate, naphthalenesulfonate, phenolsulfonic acid salt, succinic acid Sulfonates, alkylphenol polyoxyethylene Sulfates, polystyrene-based phenol polyethenoxy ether Sulfates, polystyrene-based phenol polyethenoxy ethers, alkylphenol polyoxyethylene class, fatty alcohol polyethenoxy ether class, Span class, carboxyl alkyl cellulose class, tween, aluminium-magnesium silicate, xanthans.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310570913.2A CN103583525B (en) | 2013-11-13 | 2013-11-13 | Pesticide composition containing phenazino-1-carboxylic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310570913.2A CN103583525B (en) | 2013-11-13 | 2013-11-13 | Pesticide composition containing phenazino-1-carboxylic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103583525A CN103583525A (en) | 2014-02-19 |
| CN103583525B true CN103583525B (en) | 2015-03-18 |
Family
ID=50074016
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310570913.2A Expired - Fee Related CN103583525B (en) | 2013-11-13 | 2013-11-13 | Pesticide composition containing phenazino-1-carboxylic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103583525B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104222099A (en) * | 2014-07-22 | 2014-12-24 | 江苏省绿盾植保农药实验有限公司 | Biological sterilization composition and application of biological sterilization composition to prevention and treatment of crop wilt |
| CN104430354B (en) * | 2014-11-20 | 2017-04-12 | 海南江河农药化工厂有限公司 | Biopesticide with immune induce resistance function and application thereof |
| CN104886112A (en) * | 2015-05-26 | 2015-09-09 | 重庆三峡职业学院 | Hung bag injection type combined reagent for prevention and control of kiwi fruit canker and use method thereof |
| CN106962390A (en) * | 2017-05-12 | 2017-07-21 | 四川省农业科学院植物保护研究所 | One kind is used for Corn diseases and insect pests preventing and treating coating agent for seed and its preparation method and application |
| CN110235897A (en) * | 2019-06-24 | 2019-09-17 | 江西省农业科学院植物保护研究所 | A kind of anti-root-knot nematode composition and its application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101305738A (en) * | 2007-05-18 | 2008-11-19 | 叶青 | Agricultural mixed formulation constituted by chitosan oligosaccharide or the like |
| CN102893991A (en) * | 2012-10-26 | 2013-01-30 | 肇庆市真格生物科技有限公司 | Plant growth regulating agent composition |
-
2013
- 2013-11-13 CN CN201310570913.2A patent/CN103583525B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101305738A (en) * | 2007-05-18 | 2008-11-19 | 叶青 | Agricultural mixed formulation constituted by chitosan oligosaccharide or the like |
| CN102893991A (en) * | 2012-10-26 | 2013-01-30 | 肇庆市真格生物科技有限公司 | Plant growth regulating agent composition |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103583525A (en) | 2014-02-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102893991A (en) | Plant growth regulating agent composition | |
| CN103583525B (en) | Pesticide composition containing phenazino-1-carboxylic acid | |
| CN104855385A (en) | Bactericidal composition containing chitosan oligosaccharide and fluopicolide | |
| CN102204550B (en) | Sterilization composite containing metalaxyl-M and application thereof | |
| CN103271094A (en) | Compound bactericide | |
| CN104381268A (en) | Compound bactericide | |
| CN102197819B (en) | Bactericidal composition and application thereof | |
| CN104430370A (en) | Fungicidal composition | |
| CN101700021A (en) | Composition for plant protection | |
| CN107509729A (en) | A kind of composition of coordinate plant growth and its application | |
| CN104430375A (en) | Fungicidal composition | |
| CN107897198B (en) | Compound composition of zhongshengmycin and eugenol and application thereof | |
| CN104396962B (en) | Composition pesticide | |
| CN102057923A (en) | Germicide composition | |
| CN103651396B (en) | Sterilization composition | |
| CN105104389A (en) | Composition containing active ester and chitosan oligosaccharide, and applications thereof | |
| CN1792163A (en) | Mixed agricultural chemicals for anti-tobacco virus | |
| CN103636619A (en) | Compound bactericide | |
| CN108669079A (en) | It is a kind of to be used to prevent Boscalid and eugenol compound pesticide preparation of eliminating vegetable botrytis and preparation method thereof | |
| CN104430384B (en) | A kind of bactericidal composition | |
| CN103975922B (en) | A kind of anti-plant virus composition and application thereof | |
| CN103651395B (en) | Sterilization composition | |
| CN103651398B (en) | Compound bactericide | |
| CN101766190B (en) | Housefly larvae chitooligosaccharide preparation for preventing and controlling tobacco leaf diseases and application thereof | |
| CN104430373A (en) | Fungicidal composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150318 Termination date: 20151113 |
|
| EXPY | Termination of patent right or utility model |