CN104222099A - Biological sterilization composition and application of biological sterilization composition to prevention and treatment of crop wilt - Google Patents
Biological sterilization composition and application of biological sterilization composition to prevention and treatment of crop wilt Download PDFInfo
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- CN104222099A CN104222099A CN201410351377.1A CN201410351377A CN104222099A CN 104222099 A CN104222099 A CN 104222099A CN 201410351377 A CN201410351377 A CN 201410351377A CN 104222099 A CN104222099 A CN 104222099A
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Abstract
The invention discloses a biological sterilization composition and an application of the biological sterilization composition to prevention and treatment of crop wilt. Active components of the biological sterilization composition comprise 60% of amino-oligosaccharin and 95% of phenazine-1-carboxylic acid according to a certain mass ratio. The biological sterilization composition has remarkable effects on the prevention and the treatment of strawberry, tomato and watermelon wilts.
Description
Technical field
The present invention relates to pesticide field, relate to a kind of complex biological bactericidal composition and the application in control crop fusarium wilt thereof specifically.
Background technology
Amino-oligosaccharide refers to the oligosaccharide that D-Glucosamine connects with β-1.4 glycosidic bond, and after obtaining shitosan by chitin degrading, degraded obtains again, or the low toxicity bactericide extracted by fermentable.Amino-oligosaccharide can produce inhibitory action to the growth of some germs, affect fungus spore germination, brings out that hypha form morphs, biochemistry changes in spore.Can excite plant vivo gene, produce and have the chitinase of resistant effect, dextranase, guarantor's element and PR albumen etc., and have cell activation effect, the recovery of the plant that contributes to being injured, short root strong sprout, strengthen the resistance of crop, Promoting plant growth is grown.Control fruit tree, vegetables, underground rhizome, tobacco, the virus of traditional Chinese medicine and grain and cotton crop, bacterium, fungus-caused multiple diseases can be widely used in.
Shen piperazine mycin: chemical name is phenazine-1-carboxylic acid, molecular formula is C
13h
8n
2o
2, the Shen Qin mycin Ministry of Agriculture is registered in watermelon blight and capsicum epidemic disease, also has favorable effect to virus disease.There is efficient, low toxicity, wide spectrum, nuisanceless and good with Environmental compatibility feature, more than 80% is reached to the average control efficiency such as fusarium wilt, epidemic disease, blight dis-ease and root rot.The fusarium wilt of strawberry, tomato and watermelon, the blight dis-ease of muskmelon, the epidemic disease, root rot etc. of capsicum are the chronic diseases of very difficult radical cure during current gourd, fruit and vegetable is produced, there is the area that gourd, fruit and vegetable fungoid root-rot and stem rot etc. fall ill and exceed more than 2,500 ten thousand mu times in China, economic loss reaches billions of unit every year.Long-term next, the Main Means preventing and treating these diseases sprays the chemical agent such as " can kill ", " topsin ", and medicament is residual not only affects food security and quality, also pollutes ecotope.
Have not yet to see the Combination application report of the controlling disease comprising amino-oligosaccharide and Shen piperazine mycin plant resource bactericidal composition, to the use of Shen piperazine mycin also in the exploratory stage, certain disease not for certain crop has special use report.
Summary of the invention
Goal of the invention: the object of this invention is to provide a kind of biological antibiotic composition, and prepare the method for agricultural chemicals, and utilize the application of said composition on the filling root Prevention technology of crop fusarium wilt.
Technical scheme: in order to realize foregoing invention object, a kind of biological antibiotic composition of the present invention, its active component comprises amino-oligosaccharide and Shen piperazine mycin 1: 1 ~ 1: 7 compositions in mass ratio.
As further optimization of the present invention, the active component of this bactericidal composition comprises 60% amino-oligosaccharide and 95% Shen piperazine mycin, 1: 5 ~ 1: 7 compositions in mass ratio.
Biological antibiotic composition prepares a method for agricultural chemicals, take water as medium, and former medicine, dispersant, suspending agent and antifreezing agent etc. are joined in batching kettle and mix, after ball mill or clipper are just pulverized, porphyrize in sand milling still, makes suspending agent.
The method that the present composition can be known by oneself is prepared into applicable agricultural use suspending agent; The auxiliary agent used comprises dispersant, wetting agent, emulsifier, antifreezing agent, thickener etc. and other is of value to the known substance that active ingredient is stablized in the formulation and drug effect plays, be all various compositions that are conventional in pesticidal preparations or permission use, be not particularly limited; The carrier that the present composition comprises can make fixing or liquid, can use any usually in preparation desinsection, bactericidal composition any carrier used all can use.
Surfactant can be wetting agent, dispersant or emulsifier, and they can be ionic or nonionic.Wetting agent is natural products wetting agent and cationic wetting agent, as Tea Saponin, silkworm excrement, bay alcohol radical sodium sulphate, sodium dodecyl benzenylsulfonate, pull open in powder etc. one or more; Dispersant is industrial by-products and anionic dispersing agent, such as lignosulfite, lignin and derivative sulfonate thereof, one or more in the bitter edible plant and alkyl bitter edible plant carbinol condensation thing sulfonate, alkylsulfonate; Emulsifier mainly comprises nonionic emulsifier as polyoxyethylene fatty acid ester, polyoxyethylene aliphatic alcohol ether, Polyoxyethylene fatty glue, ethoxylated castor oil and anionic emulsifier etc.
Thickener has natural in synthesis, as water-soluble high-molecular compound and water-soluble resin gum Arabic, xanthans, methylcellulose, carboxymethyl cellulose etc.; Antifreezing agent requires that resistance to frost is good, and volatility is low and little to active ingredient solvability, and conventional has ethylene glycol, propane diols, glycerine etc.; Defoamer comprises organosilicon ketone, C
8-10 fatty alcohols, C
iO-20 representative examples of saturated aliphatic carboxylic and ester class, ester one ether type compound etc., conventional organic silicone and ester-ether type defoamer.
Suitable liquid-carrier comprises water and organic solvent.Water can be used as solvent or thinner, and organic solvent also can be used as auxiliary cosolvent or stabilization additives, and suitable organic solvent comprises aromatic hydrocarbons, such as toluene, dimethylbenzene, benzene Fluhyzon and chlorinated aromatic hydrocarbons, chlorinated aliphatic hydrocarbons, aliphatic hydrocarbon.Particularly suitable be polar solvent, the isopropyl alcohol of such as alcohols, butanols, ethylene glycol, cyclohexanol, ketone such as acetone, MEK, cyclohexanone, dimethyl formamide, dimethyl sulfoxide (DMSO) and N monomethyl adjoin pyrrolidone.Different liquid mixtures is often applicable.
Amino-oligosaccharide used in the present invention can obtain by the extraction processing technology of maturation and production technology, and Yuan Yao enterprise produced by domestic existing female medicine at least 3.Shen piperazine mycin has very ripe production technology at present in China, and the former medicine supply of Shanghai Nong Le pesticide chemical Co., Ltd is more sufficient.Its access approaches is unimportant, as long as meet ratio range of the present invention and application conditions.
The present invention utilizes above-mentioned composition to prevent and treat in the fusarium wilt of traditional crop and obtains remarkable result, adopts following steps:
(1) amino-oligosaccharide and Shen piperazine mycin are dissolved in acetone respectively, make 10000ug/ml mother liquor for subsequent use;
(2) above-mentioned mother liquor is diluted 500 ~ 2000 times to crop root irrigation.
Described crop is watermelon, strawberry or tomato.
Preferably, described amino-oligosaccharide and Shen piperazine mycin be 1: 5 composition in mass ratio, and described mother liquor extension rate is 1500 times.
Should be noted that, crop of the present invention includes but not limited to watermelon, strawberry or tomato.But the present invention is mainly by the research of apply at agriculture field amino-oligosaccharide and Shen piperazine mycin complex composition, be provided for control strawberry, watermelon, tomato crop the disease such as fusarium wilt with the bactericidal composition of amino-oligosaccharide and Shen piperazine mycin.For other because of similar germ crop fusarium wilt, based under identical inventive principle, its technology should be considered as in protection scope of the present invention.But through practice, think that current said composition is specially adapted to prevent and treat the disease such as fusarium wilt of strawberry, watermelon, tomato crop.
Beneficial effect: amino-oligosaccharide of the present invention and Shen piperazine mycin compounded combination have following clear superiority:
1. show as building performance.Shen piperazine moldin is to the mechanism of action of fungal disease, mainly utilize its redox ability, active oxygen is tired out in fungal cell's inner product, suppress the oxidative phosphorylation breathing transmission chain in mitochondria, thus suppress the normal growth of mycelia, cause the fracture of plant pathogenic fungi filament, swollen, distortion and cracking; And amino-oligosaccharide can produce inhibitory action to the growth of some germs, affect fungus spore germination, bring out that hypha form morphs, biochemistry changes in spore.Can excite plant vivo gene, produce and have the chitinase of resistant effect, dextranase, guarantor's element and PR albumen etc., and have cell activation effect, the recovery of the plant that contributes to being injured, short root strong sprout, strengthen the resistance of crop, Promoting plant growth is grown.Therefore, the two mechanism of action to germ is different, composite performance synergy.
2. the consumption reduced compared with single dose after composite.By China's pesticide toxicity grading criteria, amino-oligosaccharide is micro-toxicity, through certified authority test products to rat acute per os, through skin
to eye, no skin irritation, sensitization rate is 0, belongs to weak sensitizer.Field is to honeybee, bird avirulence; Shen piperazine mycin belongs to low toxicity bactericide, acute oral LD
50rat > 5000mg/kg, acute through skin LD
50rat > 2000mg/kg.Without teratogenesis, carcinogenic, mutagenesis, to people, animal, crop safety; Fish, honeybee, silkworm etc. are all had no adverse effects.After dispenser 7 days, basic detection supplemented residue.Reduce the consumption of the mould fungicide of Shen piperazine after both are composite, improve control efficiency, toxicity is lower.
3. the fusarium wilt etc. of the crop such as amino-oligosaccharide and Shen piperazine mycin built composition for preventing and controlling strawberry, watermelon, tomato.Because current chemical bactericide has produced pesticide resistance in various degree as disliked mould spirit, thiophanate-methyl, carbendazim, polyoxin etc. to crop pests such as the fusarium wilts of strawberry, watermelon, tomato.
In order to understand invention essence better, describe the technology contents of invention below in detail with specific embodiment, but purposes of preventing and treating of the present invention is not limited thereto.Percentage in the following example is the mass percent in preparation.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described.
Embodiment 1
A kind of biological antibiotic composition, active component to be made up of in mass ratio 60% amino-oligosaccharide and 95% Shen piperazine mycin at 1: 1.
By recipe requirements, take water as medium, former medicine, dispersant, suspending agent and antifreezing agent etc. are joined in batching kettle and mixes, after ball mill or clipper are just pulverized, porphyrize in sand milling still, makes suspending agent, through after the assay was approved, carry out metering, distributing, packaging and finished product.
Embodiment 2
A kind of biological antibiotic composition, active component to be made up of in mass ratio 60% amino-oligosaccharide and 95% Shen piperazine mycin at 1: 3.
By recipe requirements, take water as medium, former medicine, dispersant, suspending agent and antifreezing agent etc. are joined in batching kettle and mixes, after ball mill or clipper are just pulverized, porphyrize in sand milling still, makes suspending agent, through after the assay was approved, carry out metering, distributing, packaging and finished product.
Embodiment 3
A kind of biological antibiotic composition, active component to be made up of in mass ratio 60% amino-oligosaccharide and 95% Shen piperazine mycin at 1: 5.
By recipe requirements, take water as medium, former medicine, dispersant, suspending agent and antifreezing agent etc. are joined in batching kettle and mixes, after ball mill or clipper are just pulverized, porphyrize in sand milling still, makes suspending agent, through after the assay was approved, carry out metering, distributing, packaging and finished product.
Embodiment 4
A kind of biological antibiotic composition, active component to be made up of in mass ratio 60% amino-oligosaccharide and 95% Shen piperazine mycin at 1: 7.
By recipe requirements, take water as medium, former medicine, dispersant, suspending agent and antifreezing agent etc. are joined in batching kettle and mixes, after ball mill or clipper are just pulverized, porphyrize in sand milling still, makes suspending agent, through after the assay was approved, carry out metering, distributing, packaging and finished product.
Above-mentioned four embodiments are in control crop, especially watermelon, strawberry, tomato wilt, and during root irrigation, step is as follows:
(1) amino-oligosaccharide and Shen piperazine mycin are dissolved in acetone respectively, make 10000ug/ml mother liquor for subsequent use;
(2) above-mentioned mother liquor is diluted 500 ~ 2000 times to crop root irrigation.
Test example 1
strains tested
Strawberry Sclerotium rolfsii, by Zhenjiang Institute of agricultural sciences be separated, qualification and according to Ke Hao's rule (Koch, s Rule)] checking after and save backup.Bacterial strain is stored in (4 DEG C) on PDA inclined-plane.
reagent agent
Reagent agent is 60% amino-oligosaccharide (promise biochemical industry You Qu company of Weifang, Shandong China produces); 95% Shen piperazine moldin (Shanghai Nong Le biological products Co., Ltd provides) is dissolved in acetone respectively, makes 10000ug/ml mother liquor for subsequent use.
complex composition proportion design
Carry out proportion design by preliminary experiment and the virulence curve (sod cultivation as described later) of the single dose measured, carry out composite according to the compound proportion of table 1, each built agent is all dissolved in acetone, makes 10000ug/ml mother liquor for subsequent use.
Table 1 amino-oligosaccharide and Shen piperazine mycin compound proportion scope and composite illustration
Note: in table, listed ratio all calculates by medicament active ingredient.
Then above-mentioned former medicine and built agent are diluted to series concentration liquid by table 2 for subsequent use.
Table 2 each medicine series diluted concentration gradient
instrument and equipment
Electronic balance (sensibility reciprocal 0.1mg), GZP-300A incubator, diameter are the culture dish, triangular flask, pipettor, pipette, ear washing bulb, card punch, vaccinating lancet etc. of 75mm.
sensitivity Detection
Adopt mycelial growth rate method, the Strawberry Fusarium Wilt of preservation is transferred in PDA plate, 96h is activated at 25 DEG C, then produce at nearly colony edge card punch the bacterium cake that diameter is 5mm, and be transferred in the pastille of the preparation of doubling dilution in 1.4 and the PDA plate of blank, cultivate 96h for 25 DEG C, when bacterium colony in contrasting grows to 4/5 of about plate diameter, adopt right-angled intersection method to measure colony diameter.
Calculate colony diameter average, and calculate mycelial growth average inhibition according to the following formula: mycelial growth average inhibition={ (contrast colony diameter average-process colony diameter average)/(contrast colony diameter average-inoculation bacterium cake diameter) } × 100%.Adopt DPS13.0 professional version data handling system, calculate regression equation, EC50 and 95% confidence limit thereof that each medicament suppresses Strawberry Fusarium Wilt mycelial growth, and obtain relative virus force index with the EC50 value of most sensitive medicaments for contrasting.
Calculate the synergistic effect of mixed agent according to the method for Wadley, i.e. SR < 0.5, then two kinds of medicament mixtures have antagonism; 0.5≤SR≤1.5, then two kinds of medicament mixtures have summation action; SR > 1.5, then two kinds of medicament mixtures have synergistic effect.
results and analysis:
Amino-oligosaccharide and Shen piperazine mycin and built agent thereof to Strawberry Fusarium Wilt virulence regression equation y=a+bX, correlation coefficient r, and concentration EC in effectively suppressing
50(ug/ml) and the coefficient of synergism SR of mixture in table 3.
As can be seen from Table 3, piperazine mycin in Shen is higher than amino-oligosaccharide to the bacteriostatic activity of strawberry Fusarium oxysporum; Its composite rear coefficient of synergism is synergistic effect.In addition, these two kinds of pharmacy effect mechanism are different, there is not mutual resistance problems, therefore this two classes medicament mixture can improve control efficiency, reduce effective ingredient dosage, reduce cost accounting, extend the service life of medicament.
Recipe determination result in table 3 amino-oligosaccharide and Shen piperazine mycin and compound sterilizing composed chamber thereof
For verifying the practical effect of complex preparation control crop strawberry, watermelon, tomato wilt bacterium further, carry out the demonstration test of preventing and treating wilt.
Test example 2 amino-oligosaccharide and Shen piperazine mycin and compound sterilizing combination preparation field control effectiveness test thereof
The field control effectiveness test of complex preparation to crop fusarium wilt is designed by " the pesticide field efficacy medicine test criterion " of the Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC chief editor.
Experimental scheme:
Specific experiment design is in table 4:
Table 4 amino-oligosaccharide and Shen piperazine mycin and compound sterilizing combination preparation field control effectiveness test thereof design
Test medicine:
2% 1000 times, amino-oligosaccharide aqua, Beijing Multigrass Formulation Co., Ltd. produces.
1% Shen piperazine mycin suspending agent 500 times, Shanghai Nong Le biological products Co., Ltd produces.
(active ingredient: 1% amino-oligosaccharide+1% Shen piperazine mycin, Zhenjiang academy of agricultural sciences develops 2% amino-oligosaccharide Shen piperazine mycin suspending agent 500 times.
4% amino-oligosaccharide Shen piperazine mycin suspending agent 1000 times (active ingredient: 1% amino-oligosaccharide+3% Shen piperazine mycin), Zhenjiang academy of agricultural sciences develops.
6% amino-oligosaccharide Shen piperazine mycin suspending agent 1500 times (active ingredient: 1% amino-oligosaccharide+5% Shen piperazine mycin), Zhenjiang academy of agricultural sciences develops
8% amino-oligosaccharide Shen piperazine mycin suspending agent 2000 times (active ingredient: 1% amino-oligosaccharide+7% Shen piperazine mycin), Zhenjiang academy of agricultural sciences develops.
Contrast medicament:
70% dislikes mould clever soluble powder, 3000 times of liquid, and Shaanxi Meibang Pesticide Co., Ltd. produces.
Community is arranged:
Small plot experiment, every community 50 strain, often processes and repeats for 3 times.Root dispenser is filled with, with the every strain of amount of liquid medicine 200 milliliters when field planting.
Disease survey:
Within after medicine 50 days, investigate and computational methods according to the general investigation grade scale of country, calculate average preventive effect (see table 5) respectively.
Other influences to crop:
Whether have poisoning produce, have poisoning then to record poisoning degree if observing:
Experimental result
Each medicament is to the field control effect of crop fusarium wilt:
Evaluating drug:
Amino-oligosaccharide and the Shen fusarium wilt of piperazine mycin complex preparation to strawberry, tomato, watermelon crop all have good control efficiency, pole is significantly higher than 1000 times, 2% amino-oligosaccharide aqua, the preventive effect of 1% Shen piperazine mycin suspending agent, 500 times of single preparations and the preventive effect of contrast medicament, and each compound proportion effectively can prevent and treat the harm of crop wilt to crop.
Observe according to system after medicine, have no obvious poisoning during reagent and occur, each test medicine has no adverse effects to plant growth.
Table 5 amino-oligosaccharide and Shen piperazine mycin and complex preparation thereof prevent and treat crop fusarium wilt field efficacy experimental result
Table 5 preventive effect is 3 mean values repeated.
Claims (4)
1. a biological antibiotic composition, is characterized in that: the active component of this bactericidal composition comprises amino-oligosaccharide and Shen piperazine mycin 1:1 ~ 1:7 composition in mass ratio.
2. a kind of biological antibiotic composition according to claim 1, is characterized in that: the active component of this bactericidal composition comprises 60% amino-oligosaccharide and 95% Shen piperazine mycin 1:5 ~ 1:7 composition in mass ratio.
3. a kind of biological antibiotic composition prepares the method for agricultural chemicals as claimed in claim 1 or 2, it is characterized in that: take water as medium, former medicine, dispersant, suspending agent and antifreezing agent etc. are joined in batching kettle and mixes, after ball mill or clipper are just pulverized, porphyrize in sand milling still, makes suspending agent.
4. the application of a kind of biological antibiotic composition as claimed in claim 1 or 2 when preventing and treating crop fusarium wilt root irrigation, is characterized in that:
(1) amino-oligosaccharide and Shen piperazine mycin are dissolved in acetone respectively, make 10000 ug/ml mother liquors for subsequent use;
(2) above-mentioned mother liquor is diluted 500 ~ 2000 times to crop root irrigation;
Described crop is watermelon, strawberry or tomato.
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| CN201410351377.1A CN104222099A (en) | 2014-07-22 | 2014-07-22 | Biological sterilization composition and application of biological sterilization composition to prevention and treatment of crop wilt |
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| CN201410351377.1A CN104222099A (en) | 2014-07-22 | 2014-07-22 | Biological sterilization composition and application of biological sterilization composition to prevention and treatment of crop wilt |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106105450A (en) * | 2016-06-28 | 2016-11-16 | 南京农业大学 | Method associated with the soil treatment of a kind of prevention and control crop droop and biological organic fertilizer |
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| CN1369566A (en) * | 2002-02-08 | 2002-09-18 | 上海交通大学 | Culture medium of bacteria for producing phenazine-1-carboxylic acid and process for preparing phenazine-1-carboxylic acid |
| CN103444721A (en) * | 2013-09-05 | 2013-12-18 | 肇庆长龙生物科技有限公司 | Sterilization pesticide composition, pesticide preparation and application of pesticide preparation |
| CN103583525A (en) * | 2013-11-13 | 2014-02-19 | 长江大学 | Pesticide composition containing phenazino-1-carboxylic acid |
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2014
- 2014-07-22 CN CN201410351377.1A patent/CN104222099A/en active Pending
Patent Citations (3)
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| CN1369566A (en) * | 2002-02-08 | 2002-09-18 | 上海交通大学 | Culture medium of bacteria for producing phenazine-1-carboxylic acid and process for preparing phenazine-1-carboxylic acid |
| CN103444721A (en) * | 2013-09-05 | 2013-12-18 | 肇庆长龙生物科技有限公司 | Sterilization pesticide composition, pesticide preparation and application of pesticide preparation |
| CN103583525A (en) * | 2013-11-13 | 2014-02-19 | 长江大学 | Pesticide composition containing phenazino-1-carboxylic acid |
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| CN106105450A (en) * | 2016-06-28 | 2016-11-16 | 南京农业大学 | Method associated with the soil treatment of a kind of prevention and control crop droop and biological organic fertilizer |
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Application publication date: 20141224 |