CN104585195B - Tetramycin complex and application - Google Patents
Tetramycin complex and application Download PDFInfo
- Publication number
- CN104585195B CN104585195B CN201510058948.7A CN201510058948A CN104585195B CN 104585195 B CN104585195 B CN 104585195B CN 201510058948 A CN201510058948 A CN 201510058948A CN 104585195 B CN104585195 B CN 104585195B
- Authority
- CN
- China
- Prior art keywords
- tetramycin
- compound
- application
- active material
- amides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to biological pesticide preparaton research and development field, specifically, relate to compound and the application of tetramycin and amides compound, be Application No. 2013106143470, filing date on November 26th, 2013, the divisional application of invention entitled " tetramycin complex and application ".This tetramycin and the compound of amides compound, including: tetramycin and active material；Described active material is amide-type, and described amide-type includes one or more of: benzamides, dicarboximide class, benzamide type, strobilurins, N halogenmethylthio dicarboximide class, dimethomorph.The tetramycin of present invention offer and the compound of amides compound and application compared with prior art, reduce refractory fungal bacterial strain optionally danger, the service life of prolongation active component, expand prevention and treatment range；Improve protection effect；Can delay or overcome the resistance to the action of a drug of germ；Not only save cost but also reduce the application times of agricultural chemicals.
Description
The present patent application is Application No. 2013106143470, filing date on November 26th, 2013, invention entitled
The divisional application of " tetramycin complex and application ".
Technical field
The present invention relates to biological pesticide preparaton research and development field, in particular to tetramycin and amides compound
Compound and application.
Background technology
The pesticide species being presently used for plant protection is various, and mostly pesticide species is meddlesome, but also brings a lot of problem.
Mostly agricultural chemicals is that a worm one is controlled, a medicine one is controlled, thus, agricultural chemicals can only be prevented and treated for a kind of insect pest or disease, the poison to other
Evil or disease do not produce effect, even cause the accident of the underproduction, have even ruin field replay, No kernels or seeds are gathered, as in a year of scarcity.A this worm one controls,
The plant protection technology that one medicine one is controlled has been not suitable with the needs of agricultural development, and peasant controls in the urgent need to a medicine more, disease pest double is controlled, save
Medicine saving of labor, guarantor produce volume increase, easy to use, the dispenser new technology of remarkable benefit.
Summary of the invention
It is an object of the invention to provide tetramycin and the compound of amides compound and application, to solve above-mentioned asking
Topic.
Embodiments providing the compound of a kind of tetramycin and amides compound, including: tetramycin and work
Property material；Described active material is amide-type, and described amide-type includes one or more of: benzamides, dicarboximide
Class, benzamide type, strobilurins, N-halogenmethylthio-dicarboximide class, dimethomorph.
The embodiment of the present invention additionally provides the application of a kind of tetramycin and the compound of amides compound, plants for preventing and treating
Thing disease, insect pest and weeds, and/or for preventing and treating fungi in timber, paper, leather, fiber, composite, bacterium, worm
Evil.
Compared with prior art, tetramycin possesses sterilization, prevents for the tetramycin complex of embodiment of the present invention offer and application
The effect of bacterium, the disease of curing the disease, after tetramycin and active material are compounding, by by have the active material of the different mechanism of action and its
Combination, reduction refractory fungal bacterial strain optionally danger, the service life of prolongation active component, add killing of single component
Bacterium is composed, and expands prevention and treatment range；Improve protection effect；Can delay or overcome the resistance to the action of a drug of germ；Not only save cost but also drop
The low application times of agricultural chemicals, active response country advocates green and environment-friendly pesticide.
Detailed description of the invention
Below by specific embodiment, the present invention is described in further detail, content mentioned in embodiment
Not limitation of the invention, material prescription select can suit measures to local conditions and on result without substantial impact.In these embodiments
In, unless otherwise indicated, all percentages are all weight percentage.
Tetramycin includes with the compound of amides compound: tetramycin and active material；Described active material is acid amides
Class, described amide-type includes one or more of: benzamides, dicarboximide class, benzamide type, strobilurins, N-halogen
For methyl mercapto-dicarboximide class, dimethomorph.
The embodiment of the present invention additionally provides the application of a kind of tetramycin and the compound of amides compound, plants for preventing and treating
Thing disease, insect pest and weeds, and/or for preventing and treating fungi in timber, paper, leather, fiber, composite, bacterium, worm
Evil.
Biological pesticide tetramycin is a kind of high-effective broad-spectrum fungicide, to the various plants on the crops such as cereal, fruit tree, vegetables
Disease is respectively provided with protection and therapeutic activity, especially to gray mold crops such as () tomato, cucumber, capsicums, powdery mildew (wheat class, melon,
Fruit tree etc.), the fungal disease such as scab (apple, pears etc.) there is good prevention effect.But this series bactericidal agent action site list
One, germ easily produces adaptive variation to medicament, makes the preventive effect of medicament reduce.Can rise after tetramycin is compounding with active material
To synergistic function, reduction refractory fungal bacterial strain in combination by the active material that will have the different mechanism of action selects
Property danger, extend active component service life, add the fungicidal spectrum of single component, expand prevention and treatment range；Improve
Protection effect；Can delay or overcome the resistance to the action of a drug of germ；Not only save cost but also reduce the application times of agricultural chemicals, actively rung
Country should advocate green and environment-friendly pesticide.
It follows that the present invention will describe this tetramycin complex, and tetramycin in detail by some specific embodiments
The concrete applicable cases of compound:
Tetramycin and the compound of amides compound, including: tetramycin and active material；
Described active material is amide-type, and described amide-type includes one or more of: benzamides, dicarboximide
Class, benzamide type, strobilurins, N-halogenmethylthio-dicarboximide class.
In certain embodiments, when active material includes benzamides, benzamides includes one or more of: benzene
Frost spirit, metalaxyl, R-metalaxyl, Wakil, chlorine worm benzamide；
In certain embodiments, when active material includes dicarboximide class, dicarboximide class includes following one
Or multiple: chlozolinate, sclex, isopropyl are fixed, myclozolin, procymidone, vinclozolin；
In certain embodiments, when active material includes benzamide type, benzamide type includes one or more of: wither
Rust spirit, first furan anilide, flutolanil, mebenil, oxycarboxin or thifluzamide, alachlor, Acetochlor, pretilachlor；
In certain embodiments, when active material includes strobilurins, strobilurins includes one or more of: nitrile
Fluoxastrobin, imines bacterium, fork amine benzamide, pyraclostrobin, ZEN 90160,2 [(2-trifluoromethyl)-pyridine-6-Ji Yangjijia
Base]-3-methoxy group-methyl acrylate, Lonster, Flumiclorac pentyl, trifloxystrobin；
In certain embodiments, when active material includes N-halogenmethylthio-dicarboximide class, N-halo first sulphur
Base-dicarboximide class includes one or more of: difoltan, captan, Euparen, fluoromide, folpet or first
Dichlofluanid；
In certain embodiments, active material is dimethomorph.
Wherein tetramycin mass concentration in the tetramycin compound with amides compound can be in 0.15-99%
Arbitrarily adjust, according to compound and the type of desirable effect, reach preferable by adjusting mass concentration (or the concentration that weighs)
Equilibrium toxicity and residual.
The rate of application of inventive mixture is 0.6-400 gram/ha.
Such as: in certain embodiments, the mass concentration of tetramycin is 0.15%, and concentration is relatively low.
In certain embodiments, the mass concentration of tetramycin is 15%, or 35%, or 50%, or 70%.
In certain embodiments, the mass concentration of tetramycin is 99%, and concentration is higher, at tetramycin and amides compound
Compound in play Main Function.
The finished product of the compound of this tetramycin and amides compound can make one or more in various forms, than
As: aqua, dispersible agent, missible oil, microemulsion, emulsion, suspension, gel formulation, ultra-low volume solvent (ULV).Make
Various forms is become to facilitate the storage of compound, transport and the subsequent use of tetramycin and amides compound.
Such as:
A) aqua
10 weight portions are compounded mixture be dissolved in 90 weight parts waters or water-soluble solvent.Or, add wetting agent or its
Its auxiliary agent, compounding mixture dissolves through water dilution, obtains the compound quality hundred of tetramycin and amides compound in this way
Proportion by subtraction content is the aqua of 10%.
B) dispersible agent
20 weight portions compound mixture be dissolved in 70 weight portion cyclohexanone, and add 10 parts per weight dispersing agent such as polyethylene
Base pyrrolidones, dilute with water obtains dispersion, obtains the compound quality hundred of tetramycin and amides compound in this way
Proportion by subtraction content is the dispersible agent of 20%.
C) missible oil
15 weight portions are compounded mixture and is dissolved in 75 parts by weight of xylene and adds calcium dodecyl benzene sulfonate and castor-oil plant
Oil ethoxylate (being 5 weight portions in each case), dilute with water obtains emulsion, obtains tetramycin and acid amides in this way
The missible oil that compound mass percentage content is 15% of compounds.
D) microemulsion
10 weight portions compound mixture be dissolved in 20 weight portion cyclohexanones and add calcium dodecyl benzene sulfonate (at every kind
In the case of be 20 weight portions), dilute with water obtains microemulsion, obtains the compound of tetramycin and amides compound in this way
Mass percentage content is the microemulsion of 5%.
E) emulsion
25 weight portions are compounded mixture and is dissolved in 35 parts by weight of xylene and adds calcium dodecyl benzene sulfonate and castor-oil plant
Oil ethoxylate (being 5 weight portions in each case).By emulsifying agent (Ultraturrax), this mixture is introduced 30 weights
Measure in part water and make equal phase emulsion.Dilute with water obtains emulsion, obtains answering of tetramycin and amides compound in this way
Join the emulsion that thing mass percentage content is 25%.
F) suspension
In the ball mill of stirring, 20 weight portions are compounded mixture and pulverizes and add 10 parts per weight dispersing agent and wetting agent
And 70 weight parts water or organic solvent, obtaining reactive compound suspension in small, broken bits, dilute with water obtains stable active ingredient
Thing suspension, obtains the suspension that compound mass percentage content is 20% of tetramycin and amides compound in this way
Liquid.
G) gel formulation
In ball mill, 20 weight portions are compounded mixture, 10 parts per weight dispersing agent, 1 weight portion gelling agent and 70 weight portions
Water or organic solvent grind and obtain delicate suspensions, and dilute with water is obtained tetramycin and amides compound in this way
Compound mass percentage content is the stabilized with mixture suspension of 20%.
H) ultra-low volume solvent
10 weight portions are compounded mixture be dissolved in 90 weight parts organic solvent such as dimethylbenzene, obtain the compounding mixing of tetramycin
Thing mass percentage content is the product used without dilution of 10%.
This tetramycin can be used for the mode controlling disease similar to other preparations, insect pest to the compound of amides compound
And weeds etc..Simultaneously compared with unitary agent, can preferably controlling disease, insect pest and weeds, play the effect of Synergistic.
Both the compound that can use tetramycin and amides compound also can use tetramycin and active material successively.
This tetramycin, with the application of the compound of amides compound, may be used for for controlling plant diseases, insect pest
And weeds, it is also used for the fungi in preventing and treating timber, paper, leather, fiber, composite, bacterium, insect pest.
This tetramycin and the compound of amides compound can as prevent and treat the plant in different seasons and plant part (fruit,
Flower, leaf, stem, stem tuber, root) on disease, insect pest, weeds, also protection plant late growing stage part encroach on from germ and for planting
Thing provides growth required energy.
The tetramycin of present invention protection generally includes with the application target crop of the compound of amides compound but does not limits
In following plants kind: cereal (wheat, barley, rye, oat, paddy rice, corn, Chinese sorghum and relevant species)；(sugar is with sweet for beet
Dish and fodder beet)；The operatic circle, drupe and berry (Kiwi berry, strawberry, raspberry, gooseberry, really mulberry, fig, pomegranate, carambola, kind
Pomegranate etc.)；Legume (Kidney bean, little inclined beans, pea, soybean, red bean)；Oilseed plant (rape, shepherd's purse, opium poppy, olive, to
Day certain herbaceous plants with big flowers, coconut, castor oil plant, cocoa bean, peanut)；Mellon plant (pumpkin, cucumber, muskmelon, balsam pear, wax gourd, watermelon)；
Fibre plant (cotton, flax, hemp, jute)；Citrus fruit (orange, lemon, glucose shaddock, bar tangerine)；Vegetables (spinach, lettuce
Bamboo shoot, asparagus, wild cabbage, carrot, onion, tomato, potato, pimiento)；Lauraceae (avocado, Chinese cassia tree, camphor) or such as tobacco,
Nut, coffee, eggplant, sugarcane, tea, pepper, trailing plant including grape, banana, grass roots and natural rubber plant with
And ornamental plant (flowers, shrub, broad leaf tree and aithullium) etc..It should be noted that this inventory is not offered as any restriction.
The tetramycin of the present invention and the compound of amides compound can also be used for preventing and treating industrial materials fungal infection (especially
By mould microbial), bacterium or insect pest, safeguard industries material reduces after occurring from fungi invasion and attack and infecting at this or eradicates
The fungal infection of industrial materials.Industrial materials include but not limited to organic and inorganic materials timber, paper, leather, natural or conjunction
Become fiber and composite, such as particieboard, glued board, wallboard etc..The present invention can be applied to this with the consumption of effectively suppression
Material.
The compounding mixture of the present invention occur in plant, seed, soil, region, material and spatial dimension disease, worm,
The prevention of crop smothering and treatment, provide growth required energy for plant simultaneously.
In use, its application form is the compound of this tetramycin and amides compound: preparaton, spray solution,
Any one in emulsion, paste, oil.Type of service depends on specifically being intended to purpose；Under certain conditions, it should be ensured that tetramycin
Finely and it is evenly distributed with the compound of amides compound.
In certain embodiments, when the application form of tetramycin Yu the compound of amides compound is preparaton, four
After the compound of mycin and amides compound and solvent and/or carrier and/or emulsifying agent and/or surfactant are blended
Use.
In certain embodiments, when using after tetramycin is blended with the compound of amides compound and solvent, solvent
Including one or more of: water, arsol (such as toluene, dimethylbenzene), paraffin (such as mineral oil fractions), alcohols are (such as first
Alcohol, butanol, amylalcohol, benzylalcohol), ketone (such as cyclohexanone, gamma-butyrolacton), pyrrolidones (NMP, NOP), acetic acid esters (ethylene glycol two
Acetic acid esters), dihydroxylic alcohols, fatty acid dimethylamides, aliphatic acid and fatty acid ester.
In certain embodiments, when using after tetramycin is blended with the compound of amides compound and emulsifying agent, breast
Agent includes one or more of: nonionic and anion emulsifier are (such as polyoxyethylene aliphatic alcohol ether, alkylsulfonate and virtue
Base sulfonate)；
In certain embodiments, use after tetramycin is blended with the compound of amides compound and surfactant
Time, surfactant includes one or more of: lignosulphonic acid, naphthalene sulfonic acids, phenolsulfonic acid, the alkali metal of dibutyl naphthalenesulfonic acid
Salt, alkali salt, ammonium salt, alkylaryl sulfonates, alkyl sulfate, alkylsulfonate, aliphatic alcohol sulfate, aliphatic acid and
Sulphated fatty alcohol glycol ether, sulfonated naphthalene and naphthalene derivatives and the condensation product of formaldehyde, naphthalene or naphthalene sulfonic acids and phenol and formaldehyde
Condensation product, NONIN HS 240, ethoxylated isooctyl-phenol, octyl phenol, nonyl phenol, alkyl phenyl polyglycol ether,
Tributyl phenyl polyglycol ether, three stearyl phenyl polyglycol ethers, alkyl aryl polyether alcohol, alcohol and fatty alcohol/oxidation second
Alkene condensate, ethoxylated castor oil, polyoxyethylene alkyl ether, ethoxylation polyoxypropylene, laruyl alcohol polyglycol ether acetal,
Sorbitol ester, lignin sulfite waste liquor, methylcellulose.
When vegetable seeds is acted on by the compound of tetramycin Yu amides compound, preparaton can be fabricated to
Mode, in addition to above-mentioned each blending constituent, it is also possible to mix with adhesive and/or gelling agent and/or colouring agent.
When the application form of tetramycin Yu the compound of amides compound is spray solution, emulsion, paste, oil, dispersion
The material of body is that mid-boiling point arrives high boiling mineral oil fractions, such as kerosene or diesel oil, in addition with coal tar and plant or animal
Source oil, aliphatic, ring-type and aromatic hydrocarbon, such as toluene, dimethylbenzene, paraffin, tetrahydronaphthalene, alkylated naphthalene or derivatives thereof,
Methyl alcohol, ethanol, propyl alcohol, butanol, cyclohexanol, cyclohexanone, isophorone, intensive polar solvent, such as dimethyl sulfoxide (DMSO), N-methyl pyrrole
Pyrrolidone and water.
Tetramycin and the compound of amides compound use aqueous type of service can make by adding water in the application
Emulsion concentrates, paste.For preparation emulsion, paste or oil dispersion, can should by wetting agent, thickener, dispersant or emulsifying agent
Material directly or is homogenized after being dissolved in oil or solvent in water.However, it is also possible to preparation is by compounding mixture, wetting agent, thickening
Agent, dispersant or emulsifying agent and suitably solvent or the concentrate of oil composition and this concentrate are suitable to dilute with water.
In certain embodiments, the compound of tetramycin and amides compound can be used for controlling disease and includes following one
Or multiple: fungal disease, bacterial disease, virosis, nematodiasis.
When the compound of tetramycin Yu amides compound is used for preventing and treating fungal disease, fungal disease includes following
One or more: Deuteromycotina fungi, Basidiomycotina fungi, Ascomycotina fungi, Zygomycotina fungi, mastigomycetes
Subphylum fungi；
When the compound of tetramycin Yu amides compound is used for preventing and treating bacterial disease, bacterial disease includes following
One or more: pseudomonas, bacillus；
When the compound of tetramycin Yu amides compound is used for preventing and treating virosis, virosis includes following one or many
Kind: double-stranded DNA virus, single-stranded DNA viruses, diplornavirus, single strand RNA virus；
When the compound of tetramycin and amides compound is for pest control, insect pest includes one or more of:
Insect, mite class, snail, muroid；
When the compound of tetramycin and amides compound is for controlling weeds, weeds include one or more of:
Grass, cyperus, broad-leaf weeds；
The specific embodiment of several tetramycin and the compound of amides compound is given below, it should be appreciated that these are implemented
Example is only used for further illustrating the present invention, rather than limits the scope of the present invention, without departing from the above-mentioned technology of the present invention
Under the state of mind, the various replacements made according to ordinary skill knowledge and customary means or change, all should be included in this
In the range of invention.
Example one tetramycin compounds with procymidone
Wherein, active material component selects procymidone.Procymidone is interior absorption fungicide, to Botrytis and sclerotinite
Belonging to fungi and have special efficacy, can prevent and treat fruit tree, the gray mold of vegetable crop, sclerotiniose, the fungi that benzimidazole produces resistance also has
Effect.Use that rear protected effect is good, the lasting period is long, scab developmenting spread can be stoped.
The embodiment of the present invention is the method using Toxicity Determination and field test to combine.First pass through indoor virulence to survey
Fixed, after clear and definite two kinds of medicaments are compounding by a certain percentage synergy ratio (SR), SR ＜ 0.5 is antagonism, 0.5≤SR≤1.5
For summation action, SR ＞ 1.5 is synergistic effect, on this basis, then carries out field test.
After prerun determines each medicament effective inhibition concentration scope, medicament is set at 5 dosage respectively by active constituent content
Reason, if clear water comparison.Carry out with reference to " farm-chemical indoor determination test rule bactericide ", use mycelial growth rate method to measure
The medicament virulence to crop germ.Measure bacterium colony footpath by right-angled intersection method after 72h, calculate each process net growth, mycelial growth
Inhibiting rate.
Net growth (mm)=measurement colony diameter-5
Mycelial growth inhibition rate (%)=control group net growth-process group net growth/control group net growth
Mycelial growth inhibition rate is converted into probit value (y), and liquor strength (μ g/mL) is converted into logarithm value (x), with minimum
Square law tries to achieve virulence regression equation (y=a+bx), and thus calculates the EC50 value of every kind of medicament.Simultaneously according to Wadley method
Calculating two medicaments different ratio synergy ratio (SR), SR ＜ 0.5 is antagonism, and 0.5≤SR≤1.5 are summation action,
SR ＞ 1.5 is synergistic effect.
Computing formula is as follows:
SR=EC50(th)/EC50(ob)
Wherein: a, b are respectively the ratio that tetramycin and procymidone are shared in combination；A is tetramycin, and B is procymidone.
Tetramycin is compounding with procymidone to cucumber downy mildew Toxicity Determination.
Experimental design: determine that through pilot study the effectively suppression of tetramycin, procymidone and the two different ratio mixture is dense
Degree scope.
Toxicity test result
The compounding toxicity test analysis of results table to cucumber downy mildew of table 1 0.15% tetramycin, procymidone
As shown in Table 1, tetramycin, procymidone is respectively 2.57mg/L and 0.51mg/L to the EC50 of cucumber downy mildew.Four
Mycin, when procymidone joins 80: 1 to 1: 80, synergy ratio SR is all higher than 1.5, illustrates that both tetramycin procymidones are 80: 1 to 1
: in the range of 80, mixture all shows synergistic effect, and especially when the proportioning of the two is 20: 1 to 1: 10, synergistic effect is the brightest
Aobvious prominent, wherein when tetramycin procymidone weight ratio is 10: 1, synergy ratio SR is maximum, and synergistic effect is the most obvious.
As a example by the tetramycin compound 42% tetramycin procymidone with amides compound
Formula: tetramycin 40%, procymidone 2%, 3-methyl cyclohexanol ketenes 5%, diethylene glycol (DEG) 6%, xanthans 3%, fat
Alcohol APEO 5%, aryl phenol polyoxyethylene succinate sulfonate 4%, silicone 0.5%, deionized water is supplied
100%, prepare 42% tetramycin procymidone microemulsion.
Tetramycin, procymidone and the 42% tetramycin procymidone compound preventing and treating fresh kidney beans gray mold test of pesticide effectiveness thereof
This EE is in Chaoyang County, Chaoyang City, and test medicine is provided by the research and development of Liaoning Wei Ke bio-engineering corporation, comparison
Medicament 0.3% tetramycin aqua, 50% procymidone wettable powder (commercial).
Investigating the fresh kidney beans gray mold state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, dispenser in every 7 days once, is total to dispenser 2 times.
Within after dispenser 3 days, 7 days, 14 days for the second time, investigate disease index respectively and calculate preventive effect.Experimental result is as follows:
Table 2 tetramycin, procymidone and the compound preventing and treating fresh kidney beans gray mold test of pesticide effectiveness thereof
Thus table is it can be seen that can effectively prevent and treat fresh kidney beans gray mold after tetramycin is compounding with procymidone, and prevention effect is better than
The preventive effect of single dose, and the preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
After draw through tests different local throughout the country, tetramycin and procymidone, chlozolinate, sclex, isopropyl
To the powdery mildew of all kinds of crops, droop, downy mildew, gray mold, epidemic disease, anthrax after fixed, myclozolin, vinclozolin etc. are compounding
The preventive effect of the Common Diseases such as disease, ring spot, banded sclerotial blight, spot defoliation, scab, brown rot, leaf blight, rust is all 95%
Above, being better than single dose preventive effect, synergistic effect is obvious.
Example two tetramycin and azoxystrobin compound
Wherein, active material component selects Fluoxastrobin
Embodiment 2-1 preparation method: 18% tetramycin Fluoxastrobin missible oil:
Weigh 6% tetramycin, 12% Fluoxastrobin, 4% agriculture breast 500# (calcium dodecyl benzene sulfonate), 3%Span-60# (mountain
Pears alcohol acid anhydride monostearate), 3% agriculture breast 2201#, 2% triphenyl phosphite, dimethylbenzene mends 100%.Above-mentioned raw materials is mixed
Rear fully dissolving, mixing prepares 18% tetramycin Fluoxastrobin missible oil.
Embodiment 2-2 preparation method: 24% tetramycin Fluoxastrobin suspending agent:
Weigh 4% tetramycin, 20% Fluoxastrobin, 3% agriculture breast 600,3% agriculture breast 2201,3% lignosulfonates, 0.2%
Xanthans, 6% ethylene glycol, 0.5% organic silicon defoamer, add deionized water and mend to 100%.After above-mentioned raw materials is mixed, pass through
High speed shear emulsification prepares 24% tetramycin Fluoxastrobin suspending agent.
Embodiment 2-3 preparation method: 12% tetramycin azoxystrobin microemulsion:
Weigh 3% tetramycin, 9% Fluoxastrobin, 6% emulsifying agent such as agriculture breast 500# (calcium dodecyl benzene sulfonate), 2% agriculture breast
2201#, 3% agriculture breast 600#, 1% methyl alcohol, 6% dimethylbenzene, 1.2% epoxychloropropane, 0.4% aceticanhydride, deionized water are mended extremely
100%, above-mentioned raw materials is fully dissolved mixing and prepares 12% tetramycin azoxystrobin microemulsion.
Assays Example 2-1: to the suppression test of rice sheath blight disease indoor colony growth.
Test method is carried out with reference to Wu Wenjun " chemical protection of plant experimental technique introduction " (1993).By the tetramycin of 15%
Mother liquor, the 95.4% former medicinal acetone of Fluoxastrobin, Tween-80 make 1.0 μ g/mL mother liquors, utilize mother liquor to become Fluoxastrobin, four mould
Element mass ratio is respectively 3:1～5:1, and gradient is respectively 0.01 μ g/mL, 0.02 μ g/mL, 0.04 μ g/mL, 0.08 μ g/mL
PDA plate.The concrete data of result of the test are shown in Table 3.
Table 3 Fluoxastrobin, tetramycin indoor antibacterial test data
Toxicity index and the co-toxicity coefficient (CTC value) of mixture of each medicament is calculated, when CTC≤80 are according to the abundant method of Sun Yun
Antagonism, 80 CTC 120 are summation action, and CTC >=120 are synergistic effect.
Being found by indoor bacterium colony Developing restraint test, Fluoxastrobin, tetramycin compound according to the mass ratio of 3:1～5:1
Length of being born Rhizoctonia solani Kuhn afterwards is respectively provided with synergy inhibitory action, wherein obvious with tetramycin, Fluoxastrobin 1:2 synergy,
Co-toxicity coefficient is 234.57, and the two compound synergic is notable, it is possible to the extension of significantly more efficient suppression bacterium colony.
Assays Example 2-2: prevent and treat the field control effectiveness test of rice sheath blight disease.
Reagent agent and dispenser dosage: (1) 24% tetramycin Fluoxastrobin suspending agent (preparation method embodiment 8-2), mu
With 40 grams of preparation；(2) 12% tetramycin azoxystrobin microemulsion (preparation method embodiment 2-3), 80 grams of preparation of mu.
Comparison medicament and dispenser dosage: (3) 1.5% tetramycin aquas, 40 grams of preparation of mu；(4) 10% Fluoxastrobins are wettable
Property pulvis, 20 grams of preparation of mu,
Spraying time and application method: rice sheath blight disease early stage dispenser, be watered even spraying.
The field control effectiveness test result of rice sheath blight disease prevented and treated by table 4
| Reagent agent | Preparation usage amount (g/ mu) | 7 days preventive effects (%) after medicine | 14 days preventive effects (%) after medicine |
| 1 | 40 | 74.3 | 81.3 |
| 2 | 80 | 73.8 | 80.7 |
| 3 | 40 | 66.5 | 72.5 |
| 4 | 20 | 61.5 | 71.9 |
Result of the test: be shown in Table 4.As can be seen from the test results: after dispenser 7 days, the tetramycin of the present invention mixes with Fluoxastrobin
Combo compound to rice sheath blight disease preventive effect all more than 70%, hence it is evident that be better than the single dose of comparison；After dispenser 14 days, the four of the present invention
Mycin and Fluoxastrobin mixture composition all more than 80%, are also significantly better than the single dose of comparison to rice sheath blight disease preventive effect；Test
During each medicament be showed no poisoning produce, to crop safety.Illustrate that the tetramycin of the present invention is excellent with Fluoxastrobin mixture composition
In single dose, improve actual prevention effect, reduce dosage, reduce cost, reduce the medicament adverse effect to environment, delay disease
Drug-fast generation, administers agricultural synthesis and has great significance.
Example three tetramycin compounds with chlorine worm benzamide
Wherein, active material component selects chlorine worm benzamide.Tetramycin chlorine worm benzamide compound can effectively prevent and treat rice
Seasonal febrile diseases and rice leaf roller, facilitate peasant to use.Use tetramycin chlorine worm benzamide compound, while treatment rice blast
Effectively prevent rice leaf roller, reduce the medicament of later stage use preventing and treating rice leaf roller；Even if two kinds of diseases are sent out simultaneously
Raw, complex preparation also has good therapeutic action.
The preparation of suspending agent:
According to the proportion requirement of formula, it is separately added into wetting agent, dispersant, former medicine, bleeding agent, defoamer, thickener, prevents
Freeze agent, sleep and mix, be fully ground to product average grain diameter less than 2 microns, obtain suspending agent product, see below example.
The weight ratio of each component in embodiment 3-1 suspending agent
Chlorine worm benzamide 6%, tetramycin 18%, sodium dodecylbenzenesulfonate 7%, JFC4.5%, polyvinyl alcohol 2.5%,
NNO 6%, xanthans 0.5%, propane diols 1%, S-29,0.5%, water complements to 100%.
The weight ratio of each component in example 3-2 suspending agent
Chlorine worm benzamide 29%, tetramycin 20%, Nonyl pheno base ether 5.5%, JFC4.5%, polyvinyl alcohol
2.5%, NNO8%, xanthans 0.5%, S-290.5%, water complements to 100%.
Field test detailed description of the invention:
In July, 2008, paddy rice carries out the test of pesticide effectiveness, is the occurrent time of rice blast during test, blade has scab,
The medium generation of rice leaf roller, medicament selects 20% chlorine worm benzamide suspending agent and 0.3% tetramycin aqua, for the first time after dispenser between
Second time dispenser after 10 days, for the second time 15 days investigation insecticidal effects and bactericidal effect after dispenser.
The investigation method of rice blast is: 5 fixed point observations, every two clumps of paddy rice are selected in each community, investigates disease and refer to before dispenser,
After last dispenser, investigation in 15 days sick leaf rate, disease index, calculates the prevention effect of each medicament.
Rice blast grade scale:
0 grade: anosis
1 grade: leaf spot lesion is less than 5, length is less than 1cm；
3 grades: leaf spot lesion 6-10, part scab length is more than 1cm；
5 grades: leaf spot lesion 11-25, part scab is linked to be sheet, accounts for blade area 10-25%；
7 grades: leaf spot lesion more than 26, scab is linked to be sheet, accounts for blade area 26-50%；
9 grades: scab is linked to be sheet, account for blade area more than 50% or the most withered.
Disease index=100 × ∑ (the sick numbers of sheets at different levels × relative progression)/(investigating total number of sheets × total progression)
Prevention effect (%)=[1-(CK0 × Pt1 disease index/CK1 × Pt0 disease index)] × 100
CK0 is the disease index of the front check plot of dispenser；Ck1 is the disease index of check plot after dispenser；
Pt0 is the disease index before treatment region dispenser；Pt1 is the disease index of dispenser post processing zone；
The investigation method of rice leaf roller: 15 days investigation leaf roll numbers after dispenser the last time, calculates prevention effect.
Leaf roll rate (%)=(investigation leaf roll number/investigate total leaf tree) × 100
Prevention effect (%)=[(blank district leaf roll rate-treatment region leaf roll rate)/blank district leaf roll rate] × 100
Table 5 chlorine worm benzamide, tetramycin test of pesticide effectiveness result
From test, after chlorine worm benzamide is compounding with tetramycin, rice blast and rice leaf roller are had well
Prevention effect, for the second time after dispenser 15 days preventive effects to rice blast and rice leaf roller all more than 90%.Peasant's a drug
Getting final product effectively preventing rice blast and rice leaf roller, in all having efficiently because of chlorine worm benzamide and tetramycin, absorption conduction, can show
Write the lasting period extending medicament, reduce times for spraying, reduce planting cost.In experiments it is found that, complex preparation is except volume vertical to rice
Leaf snout moth's larva has outside good prevention effect, also has planthopper and certain double controls effect, planthopper and propagate paddy rice multiple diseases, fall
Low planthopper insect population radix, is also beneficial to the preventing and treating of other disease of later stage.
Example four tetramycin compounds with carboxin
Wherein, active material component selects carboxin, and its structural formula is following (I), chemical name: 5,6-dihydro-2-methyl-
N-phenyl-Isosorbide-5-Nitrae-oxathiene-3-formamide, a kind of heterocyclic nitrogen-group fungicide with systemic action, it can penetrate into rudiment
Seed and kill intraseminal germ, load guiding principle fungi is had higher systemic activity.
Embodiment 4-1: the indoor harmacological effect of cotton seedling blight is measured by tetramycin with carboxin compound.
Subjects: cotton rhizoctonia solani
Test method: press down with reference to People's Republic of China (PRC) farm-chemical indoor determination test rule NYT 1156.2-2006
Disease fungus mycelial growth processed test Plating is carried out.Cotton rhizoctonia solani cultivation temperature is 28 DEG C, and using culture medium is PDA training
Support base.
Connecing bacterium to measure after 1 week, statistical analysis, result of the test sees table 7.
Table 6 tetramycin and carboxin compound are to cotton rhizoctonia solani measurement result
Result of the test shows, tetramycin and carboxin compound by weight 20:1～1:1, when preventing and treating cotton rhizoctonia solani
Co-toxicity coefficient, all more than 120, has synergistic effect；Wherein, when proportioning is 10:1, co-toxicity coefficient is the highest.
Embodiment 4-2:21% tetramycin carboxin suspending agent
Formula: tetramycin 20%, carboxin 1%, methyl naphthalene sulfonate formaldehyde condensate 10%, xanthans 0.4%, swelling
Soil 1%, glycerine 5%, water complements to 100%.
Embodiment 4-3:16.5% tetramycin carboxin suspending agent seed coat agent
Composition: tetramycin 15%, carboxin 1.5%, rose-red 0.3%, methyl naphthalene sulfonate formaldehyde condensate 10%, yellow
Virgin rubber 0.4%, bentonite 1%, glycerine 5%, water complements to 100%.
Embodiment 4-4:12% tetramycin carboxin suspending agent seed coat agent
Composition: tetramycin 10%, carboxin 2%, rose-red 0.3%, methyl naphthalene sulfonate formaldehyde condensate 10%, xanthan
Glue 0.4%, bentonite 1%, glycerine 5%, water complements to 100%.
The pesticidal preparations seed dressing preventing and treating cotton seedling blight field test prepared with above-described embodiment.Specifically used method is:
Above-mentioned each preparation is blended into appropriate water stir, then sows after uniformly mixing on wheat seed and drying in the shade.This test medicine
Consumption sets two concentration for the treatment of respectively according to the difference of each composition.
Result of the test such as table 7.
Table 7 tetramycin and the carboxin complex preparation seed dressing control effect testing to cotton seedling blight
Note: represent the significance of difference (p ＜ 0.05) of variance analysis with a, b, the c after column data.
Field efficacy result in table shows, tetramycin carboxin composition has well preventing and treating to cotton seedling blight
Effect, under same dose, preventive effect is better than single dose, and when wherein tetramycin carboxin proportioning is 10: 1, activity is the highest.
Conclusion: above-described embodiment shows, tetramycin carboxin is the most alone after combining to be compared, to cotton seedling blight
Prevention effect is significantly improved, and shows obvious synergistic effect.Wherein with synergistic effect during tetramycin carboxin 10: 1
The highest.
Example five tetramycin compounds with dimethomorph
Wherein, active material component selects dimethomorph.Tetramycin, the former medicine of dimethomorph are respectively prepared 20% suspending agent
Carry out indoor pot experiment.Test method is carried out with reference to Wu Wenjun " chemical protection of plant experimental technique introduction " (1993).According to
Requirement of experiment, is respectively prepared single dose, and the type of respective quality ratio.The concrete data of result of the test are shown in Table 8.
Table 8 tetramycin and dimethomorph indoor pot experiment data.
Toxicity index and the co-toxicity coefficient (CTC value) of mixture of each medicament is calculated, when CTC≤80 are according to the abundant method of Sun Yun
Antagonism, 80 CTC 120 are summation action, and CTC >=120 are synergistic effect.
Found by indoor bacterium colony Developing restraint test, after tetramycin and dimethomorph compound according to the ratio of 3:1～1:5
Cucumber downy mildew is respectively provided with synergistic effect, and wherein obvious with 1:3 synergy, co-toxicity coefficient is 227.68, the two compounding increasing
Effect property is notable.
20% tetramycin dimethomorph suspending agent:
0.3% tetramycin 4%, dimethomorph 16%, methyl naphthalene sulfonate formaldehyde condensate (dispersant) 5%, 0203B
(surfactant) 6%, white carbon (filler) 5%, aluminium-magnesium silicate (suspending agent) 0.4%, ethylene glycol (antifreezing agent) 3%, phosphorus
Acid tributyl (defoamer) 1.5%, deionized water complements to 100%.Above-mentioned formula is pulverized the most in advance, adds
Grinding in sand mill, after high speed shear mixes, allotment prepares 20% tetramycin dimethomorph suspending agent.
This formula preventing and treating cucumber downy mildew is by 2000 times of dilution sprayings, and after medicine, 7 days prevention effects are 91.3%.6% 4 is mould
Element aqua is by 1500 times of dilution sprayings, and after medicine, 7 days prevention effects are 81.2%, and 40% dimethomorph water dispersion granula presses 1500 times
Dilution spraying, after medicine, 7 days prevention effects are 69.7%.Test shows that 20% tetramycin dimethomorph suspending agent is to cucumber downy mildew
Disease has good prevention effect, is better than corresponding single dose.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, that is made any repaiies
Change, equivalent, improvement etc., should be included within the scope of the present invention.
Claims (3)
1. a tetramycin and the compound of amides compound, it is characterised in that including: tetramycin and active material；Described
Active material is amide-type, and described amide-type is following one: procymidone, carboxin, dimethomorph；
When described active material is procymidone, described tetramycin is 20:1 to 1:10 with the weight ratio of described procymidone；
When described active material is carboxin, described tetramycin is 20:1～1:1 with the weight ratio of described carboxin；
When described active material is dimethomorph, described tetramycin is 3:1～1:5 with the weight ratio of described dimethomorph.
The compound of tetramycin the most according to claim 1 and amides compound, it is characterised in that described tetramycin with
The compound of amides compound is any one or more in microemulsion, suspension.
3. the application of the compound of the tetramycin described in any one of claim 1-2 and amides compound, it is characterised in that
For controlling plant diseases.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510058948.7A CN104585195B (en) | 2013-11-26 | 2013-11-26 | Tetramycin complex and application |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510058948.7A CN104585195B (en) | 2013-11-26 | 2013-11-26 | Tetramycin complex and application |
| CN201310614347.0A CN103583532B (en) | 2013-11-26 | 2013-11-26 | Tetramycin complex and application |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310614347.0A Division CN103583532B (en) | 2013-11-26 | 2013-11-26 | Tetramycin complex and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104585195A CN104585195A (en) | 2015-05-06 |
| CN104585195B true CN104585195B (en) | 2016-08-17 |
Family
ID=53111535
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510058948.7A Active CN104585195B (en) | 2013-11-26 | 2013-11-26 | Tetramycin complex and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104585195B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638769A (en) * | 2016-04-13 | 2016-06-08 | 储志强 | Method for preparing pesticide |
| CN113367146B (en) * | 2021-05-11 | 2022-08-05 | 贵州省亚热带作物研究所 | Compound bactericidal preparation for preventing and treating coix seed leaf blight and use method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696652A (en) * | 2012-06-29 | 2012-10-03 | 青岛星牌作物科学有限公司 | Sterilizing composite for controlling pyricularia oryzae |
| CN103300024A (en) * | 2013-06-19 | 2013-09-18 | 北京燕化永乐农药有限公司 | Sterilization composition containing tetramycin |
-
2013
- 2013-11-26 CN CN201510058948.7A patent/CN104585195B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696652A (en) * | 2012-06-29 | 2012-10-03 | 青岛星牌作物科学有限公司 | Sterilizing composite for controlling pyricularia oryzae |
| CN103300024A (en) * | 2013-06-19 | 2013-09-18 | 北京燕化永乐农药有限公司 | Sterilization composition containing tetramycin |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104585195A (en) | 2015-05-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103583532B (en) | Tetramycin complex and application | |
| CN101755842B (en) | Bactericide composition containing kresoxim-methyl | |
| CN101755797A (en) | Bactericide composition containing boscalid | |
| CN104012579A (en) | Sterilization composition and application thereof | |
| CN102349514A (en) | Bupirimate-containing antibacterial composition | |
| CN103749452B (en) | A kind of seed dressing | |
| CN104604895B (en) | Tetramycin compound and application | |
| CN101715771B (en) | Sterilizing composition containing imibenconazole | |
| CN101715775B (en) | Sterilizing composition containing ethirimol | |
| CN101543218A (en) | Kresoxim-methyl and triazole bactericide composition and use thereof | |
| CN104855394B (en) | A kind of Weeding composition applied in post-emergence period of corn containing topramezone | |
| AU2014292554B2 (en) | Fungicidal mixture | |
| CN104585195B (en) | Tetramycin complex and application | |
| CN102349524B (en) | Sterilizing composition containing Cyflufenamid | |
| CN101558757B (en) | Anti-insect and anti-mite composition containing spirodiclofen and nitenpyram | |
| CN106172451A (en) | The application on preventing and treating gibberella zeaze petch of wheat and barley of the bactericidal composition containing captan and Tebuconazole | |
| CN103548847B (en) | A kind of Fungicidal composition and application thereof | |
| CN102293200B (en) | Sterilization composition containing zoxamide and copper pimaric acid | |
| CN101564041B (en) | Bactericidal composition compounded by prochloraz and pyrimethanil | |
| CN104488896A (en) | Compound composition containing methylsulfonylconazole and strobilurin fungicide and preparation | |
| CN107372528A (en) | A kind of bactericidal composition and its application containing prothioconazoles and S-Ethyl ethylthio sulfonate | |
| CN104542648B (en) | Bactericidal composition and application thereof | |
| CN108338165A (en) | A kind of microbicide compositions including emodin derivates and polysaccharide | |
| CN107232217B (en) | Bactericide composition and application thereof | |
| CN102349523A (en) | High-efficient sterilization composition containing bupirimate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 122000 Chaoyang City Chaoyang District, Longquan Province, the main street, No., No. two, No. 8 Applicant after: LIAONING WKIOC BIOENGINEERING CO., LTD. Address before: 122000 Chaoyang City Chaoyang District, Longquan Province, the main street, No., No. two, No. 8 Applicant before: LIAONING WKIOC BIOENGINEERING CO., LTD. |
|
| COR | Change of bibliographic data | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 122000 No.8, Section 2, Longquan street, Chaoyang Development Zone, Chaoyang City, Liaoning Province Patentee after: LIAONING WKIOC BIOENGINEERING Co.,Ltd. Address before: 122000 No.8, Section 2, Longquan street, Chaoyang Development Zone, Chaoyang City, Liaoning Province Patentee before: LIAONING WKIOC BIOENGINEERING Co.,Ltd. |
|
| CP01 | Change in the name or title of a patent holder |