CN102349514A - Bupirimate-containing antibacterial composition - Google Patents

Bupirimate-containing antibacterial composition Download PDF

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Publication number
CN102349514A
CN102349514A CN2011103066223A CN201110306622A CN102349514A CN 102349514 A CN102349514 A CN 102349514A CN 2011103066223 A CN2011103066223 A CN 2011103066223A CN 201110306622 A CN201110306622 A CN 201110306622A CN 102349514 A CN102349514 A CN 102349514A
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oxygen quinoline
bupirimate
bactericidal composition
preparation
benzene oxygen
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杜秀斌
葛尧伦
杨波涛
韩先正
葛大鹏
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Hailir Pesticides and Chemicals Group Co Ltd
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Hailir Pesticides and Chemicals Group Co Ltd
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Abstract

The invention relates to an antibacterial composition in the field of compounding of pesticides, and the antibacterial composition comprises two effective components (bupirimate and proquinazid or a binary mixture of quinoxyfen) and the balance of auxiliary components which are allowable and acceptable in pesticides, wherein the weight percentage of the two effective components is (90:1)-(1:90), preferably (2-65):(70-3); the total weight percent of the effective components in the antibacterial composition is 1-90%, preferably 5-70%; and the antibacterial composition can be prepared into wettable powder, a suspending agent, an emulsifiable concentrate, a soluble liquid solvent, an emulsion in water, and water dispersible granules by using known methods. The antibacterial composition disclosed by the invention is mainly used for preventing and treating diseases arising from erysiphe, puccinia, colisporites, pyrenophora and septoria bacteria.

Description

A kind of bactericidal composition that contains bupirimate
Technical field
The present invention relates to the agriculture chemical compounding technical field, be specifically related to a kind of being that active ingredient is carried out two yuan of composite bactericidal compositions one of in bupirimate and the third oxygen quinoline or the benzene oxygen quinoline.
Background technology
Bupirimate belongs to the miazines systemic fungicide, has protection and therapeutic action.Can be absorbed rapidly by plant roots, stem, leaf, and in plant corpus, run to each position, so resistance of rainwater washing against.Have that consumption is few, efficient, low toxicity, powdery mildew had advantage such as special efficacy, therefore replaced agricultural chemicals such as phonetic phenol of second and dimethirimol.Be suitable for ornamental plants, field crops such as fruit tree, vegetables, flowers.The various powdery mildews of controlling object like apple, grape, cucumber, strawberry, rose, sweet Lay powdery mildew, have poisoning to some kinds such as strawberry, apple, roses.Its chemical structural formula is:
Figure BDA0000097893420000011
The third oxygen quinoline, English common name proquinazid, chemical name 6-iodo-2-propoxyl group-3-propyl group quinazoline-4 (3H)-ketone.Molecular formula C 14H 17IN 2O 2Be mainly used in diseases such as preventing and treating powdery mildew.
Benzene oxygen quinoline, English common name quinoxyfen, chemical name 5,7-two chloro-4-(to fluorophenoxy) quinoline.Molecular formula C 15H 8CL 2FNO.Be systemic fungicide, the control of cereals powdery mildew is had special efficacy, behind the foliage application, medicament can promptly ooze the people in plant tissue, and shifts to the top, and the lasting period is long.Comprise no cross resistances such as triazole type, first hydrogen base esters of acrylic acid with existing bactericide in the market.
Powdery mildew is the plant disease that is caused by the Erysiphaceae in the fungi.Powdery mildew distributes extensively in the whole world, and the dicotyledon of causing harm is particularly general.The main powdery mildew of China has by the microbial cereal class of standing grain white powder powdery mildew; The powdery mildew of various plants such as melon, the beans that causes by Siberian cocklebur monofilament shell, crudefiber crop; The grape powdery mildew that causes by grape snag shell; The powdery mildew of more than 80 kind of trees such as the mulberry that causes by the hazel ball pin shell, pears, persimmon, walnut, chestnut, lichee, papaya papaw; Powdery mildew of the Rosa flowers that cause by rose monofilament shell etc.In the control of powdery mildew, mainly rely on medicaments such as benzimidazole, triazole type.These medicaments are because long-term use has produced pesticide resistance in various degree, and control efficiency reduces.A large amount of uses of medicament have simultaneously increased residue of pesticide, and the harm food security has increased the weight of environmental pollution.
Bupirimate is on the basis of the phonetic phenol structure of second, to carry out aminosulfonyl to melt the new pyrimidine series bactericidal agent that sends, and makes the activity of aminopyrimidine stronger, and this bactericide effect is rapider, and drug effect is more lasting.Quinolines bactericide third oxygen quinoline and benzene oxygen quinoline all have good interior suction, infiltration and conduction.I research staff of company attempts both are composite, whether has synergistic effect to observe it, has solved and has prevented and treated the problem that the powdery mildew effect is undesirable, environmental pollution is heavy in the market.Checking through a large amount of indoor biometricses and field experiment; Discovery with one of any in bupirimate and the third oxygen quinoline or the benzene oxygen quinoline be two yuan of composite bactericidal compositions of active ingredient; Preventive effect to disease improves greatly, and quick-acting, lasting period all have a distinct increment, and dosage is lower; Reduce agricultural cost, improved germicidal efficiency.Both have very strong synergistic effect experiment confirm, and about to be that active ingredient is carried out composite research one of in bupirimate and the third oxygen quinoline or the benzene oxygen quinoline, do not see relevant report at present at home and abroad as yet through retrieval.
Summary of the invention
The object of the present invention is to provide a kind of bactericidal composition to fungal disease high-efficiency environment friendlies such as powdery mildews; My research staff of company finds through indoor, outdoor formulation screening test in a large number; Composite one of in third oxygen quinoline that bupirimate that the mechanism of action is unique and effect stability, interior absorption are good or the benzene oxygen quinoline have a beyond thought synergistic effect; Can be good at solving bupirimate, the third oxygen quinoline or benzene oxygen quinoline or other bactericide such as medicaments such as benzimidazole, triazole type resistance problem in practical application to fungal diseases such as powdery mildews; And can improve crop quality, the resistance of enhancing crop, we have accomplished the present invention immediately.Be the present invention be a kind of be two yuan of combination sterilization composition preparations of active ingredient with bupirimate and the third oxygen quinoline or bupirimate and benzene oxygen quinoline, other are that agricultural chemicals is with relevant auxiliary agent and complementary component.
Bactericidal composition composition of the present invention is novel unique, has excellent specific property, wherein from the molecular structure of compound; Bupirimate relies on the nitrogen-atoms heterocycle structure of the phonetic phenol of second, carry out aminosulfonylization, has changed compound structure; Make the aminopyrimidine activity in the original structure higher; Make it have four obvious advantages: first advantage is to wait the homoiothermy animal toxicity lower to people and animals, and safer to birds, fish, second advantage is good drug efficacy; Particularly better to mycelium and conidial killing effect; The 3rd advantage is that consumption is few, and the 4th advantage is the degraded more easily in environment of this compound, and the N after the degraded etc. promotes the effect of plant growth in addition.Therefore whether the bupirimate and the third oxygen quinoline or benzene oxygen quinoline have compatibility; Whether has synergistic effect; Can not simply be equal to by the phonetic phenol of second; Hydroxyl is active different with sulfamide groups, and they are also different to influence degree of pyrimidine radicals heterocycle, whether satisfies composite requirement and also probes on the careful again experiment basis of needs.
On the basis of lot of experiment validation; We have proposed the present composition; The present invention for a kind of being that active ingredient is carried out two yuan of bactericidal composition preparations that are mixed one of in bupirimate and the third oxygen quinoline or the benzene oxygen quinoline; Wherein in the composition percentage by weight one of in the active ingredient bupirimate and the third oxygen quinoline or the benzene oxygen quinoline can be 90: 1~1: 90; On bases of some experimental formula screenings targetedly, can the percentage by weight one of in active ingredient bupirimate and the third oxygen quinoline or the benzene oxygen quinoline be preferably 2~65: 70~3.But can not explain at this point that other ratios do not have synergistic effect, just synergistic effect is more obvious in this scope.
Of the present invention being that the formulations of pesticide that two yuan of composite pesticide sterilizing composites of active ingredient can be prepared into are wetting powder, suspending agent, missible oil, soluble liquid, aqueous emulsion and water dispersible granules one of in bupirimate and the third oxygen quinoline or the benzene oxygen quinoline; Wherein the gross mass of two kinds of active ingredients accounts for 1%~90% of total formulation gross mass in the preparation; Can be preferably 5%~70%, all the other compositions are that usual auxiliaries and formulation prepare required complementary component in the agricultural chemicals.
Pesticide sterilizing composite of the present invention especially has special efficacy to the disease of cereal crops, flowers, fruit, greengrocery Erysiphe.In addition diseases such as the powdery mildews of Puccinia, Rhynchosporium spp, nuclear cavity Pseudomonas and the microbial disease of Septoria such as melon, vegetables, downy mildew, scab, leaf spot, eqpidemic disease also there is favorable effect.To the rust of beans, the red spot of banana, leaf spot, diseases such as alternaria leaf spot of apple, pear scab and grape powdery mildew all have excellent preventive effect.Bactericidal composition of the present invention all has the certain activity effect to fungal disease, more than is typical several classification and disease.
Compare other medicaments in similar effect field, bactericidal composition of the present invention also has plurality of advantages, and the first, act on rapidlyer, the lasting period is more permanent, and control efficiency is more stable; The second, delayed the drug-fast generation of crop greatly, reduced agricultural chemicals access times repeatedly, reduced the agricultural chemicals usage amount, reduce agricultural cost; The 3rd, in environment, be prone to degraded after the lasting period of this bactericidal composition, residual quantity is lower, and is friendly more to environment.Therefore the disease control to the agricultural region is significant in the application of this bactericidal composition.
Embodiment:
In order to make the object of the invention, technical scheme and advantage clearer, the present invention describes with following specific embodiment, but the present invention only limits to these examples absolutely not.The method that the present invention adopts indoor bioassay and field trial to combine.If no special instructions, the ratio of below mentioning (comprising percentage) all is a mass ratio.Should be pointed out that all any modifications of within spirit of the present invention and principle, being made, be equal to replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with accompanying claims.
Wherein prepare required necessary basis component about the formulations of pesticide and the easy manufacture implementation method following:
One of technical scheme of the present invention, described bactericidal composition are wetting powder, and the percentage by weight of component is:
Figure BDA0000097893420000031
The concrete procedure of processing of this bactericidal composition wetting powder is: press above-mentioned prescription with active principle bupirimate and the active principle third oxygen quinoline or benzene oxygen quinoline and dispersant, wetting agent and filler mixing; In stirred tank, evenly stir; Behind airslide disintegrating mill, mixing, promptly can be made into the wetting powder of the present composition.
Two of technical scheme of the present invention, described bactericidal composition are suspending agent, and the percentage by weight of component is:
The concrete production stage of this suspending agent is earlier other auxiliary agents to be mixed; Mix through high speed shear; Add active principle bupirimate and active principle third oxygen quinoline or benzene oxygen quinoline; Abrading-ball is 2~3 hours in ball crusher, makes a diameter all below 5mm, makes the suspending agent preparation of bactericidal composition of the present invention.
Three of technical scheme of the present invention, described bactericidal composition are aqueous emulsion, and the percentage by weight of component is:
Figure BDA0000097893420000042
The concrete production stage of this aqueous emulsion is: at first former medicine active principle bupirimate and the active principle third oxygen quinoline or benzene oxygen quinoline, solvent are added with emulsifier, cosolvent and be in the same place, make and be dissolved into uniform oil phase; With part water, antifreeze, other insecticides adjuvant such as antimicrobial mixes into uniform aqueous phase; When the agitated reactor high speed stirs, oil phase is added water, slowly add water, open clipper and carry out high speed shear, and add remaining water, shear half an hour approximately, form the aqueous emulsion of oil-in-water type until reaching the phase inversion point.Promptly make the aqueous emulsion of bactericidal composition of the present invention.
Four of technical scheme of the present invention, described bactericidal composition are water dispersible granules, and the percentage by weight of component is:
Figure BDA0000097893420000043
Figure BDA0000097893420000051
The concrete procedure of processing of this bactericidal composition water dispersible granules is: by above-mentioned prescription active principle bupirimate, the active principle third oxygen quinoline or benzene oxygen quinoline and dispersant, wetting agent, disintegrant and filler are mixed; Pulverize with micro jet; Through mediating; Add then and carry out granulation, drying, screening in the fluidized bed prilling dryer after sample analysis, promptly make the water dispersible granules of bactericidal composition of the present invention.
Five of technical scheme of the present invention, described Pesticidal combination are cream preparation, and the mass percent of component is:
Figure BDA0000097893420000052
The concrete production stage of this cream preparation adds emulsifier, the synergist back that stirs after the dissolving fully again and becomes the oily liquids of transparent and homogeneous for earlier active ingredient bupirimate, the third oxygen quinoline or benzene oxygen quinoline being added in the solvent; Can promptly can be made into the cream preparation of the present composition.
Described emulsifier is selected from any one or more mixtures formed with arbitrary proportion in calcium dodecyl benzene sulfonate and aliphatic acid polyethenoxy ether, APES sulfosuccinate, styryl phenol APEO, NPE, castor oil polyoxyethylene ether, aliphatic acid polyethenoxy base ester, the polyoxyethylene aliphatic alcohol ether.
Said solvent is any one or more mixed solvents formed with arbitrary proportion in xylol or biodiesel, toluene, diesel oil, methyl alcohol, ethanol, n-butanol, isopropyl alcohol, turpentine oil, solvent naphtha, dimethyl formamide, dimethyl sulfoxide (DMSO), the water equal solvent.
Described dispersant is selected from one or more in polycarboxylate, lignosulfonates, APES formaldehyde condensation products sulphate, alkyl benzene sulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, APES, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, the fatty acid glyceride APEO.
Described wetting agent is selected from: lauryl sodium sulfate, calcium dodecyl benzene sulfonate, draw back in powder copper rosinate X., wetting and penetrating agent F, alkylnaphthalene sulfonate, polyoxyethylene triphenylethylene phosphenylic acid salt, spaonin powder, silkworm excrement, the soapberry powder one or more.
Described disintegrant is selected from: one or more in bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, the sodium bicarbonate.
Described thickener is selected from: one or more in xanthans, CMC, hydroxyethylcellulose, methylcellulose, Magnesiumaluminumsilicate, the polyvinyl alcohol.
Described stabilizing agent is selected from: a kind of in sodium citrate, the resorcinol.
Described antifreezing agent is selected from: one or more in ethylene glycol, propane diols, the glycerine.
Described antifoaming agent is selected from: silicone oil, silicone compound, C 10-20Saturated fat acid compounds, C 8-10In the aliphatic alcohols compound one or more.
Described filler is selected from: one or more in kaolin, diatomite, bentonite, attapulgite, white carbon, starch, the precipitated calcium carbonate.
Indoor virulence test and field experiment
The indoor virulence measuring:
Biologicall test instance 1:
(1) experimental subjects:
The powdery mildew of cucumber germ, separation and purification gained from the powdery mildew of cucumber incidence of leaf of Shouguang, Shandong cucumber booth is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
Supply the examination medium:
PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml.
AEA medium: dusty yeast 5g/L, glycerine 20mL/L, MgSO 40.25g/L, NaNO 36g/L, KCl 0.5g/L, KH 2PO 41.5g/L, agar powder 20g/L, deionized water 1L.
(2) experimental pharmacy:
The 95% the third former medicines of oxygen quinoline, the former medicine of 95% bupirimate, sea, Qingdao synthetic institute of rel research and development centre provides.
Combination setting is mixed:
18% bupirimate, the third oxygen quinoline percentage composition was respectively 3: 15,5: 13,7: 11,9: 9,11: 7, i.e. 3%+15%, 5%+13%, 7%+11%, 9%+9%, 11%+7% totally 5 combinations that are mixed.
(3) test method:
Single agent is to the toxicity test of powdery mildew of cucumber germ:
The third oxygen quinoline and bupirimate dissolve with an amount of trichloroethanes, are made into the mother liquor of 10000 μ g/ml, preserve in 4 ℃ of following low temperature, and be for use, is diluted to a series of concentration with sterile water before the test.
The powdery mildew of cucumber germ after cultivating in advance on the AEA medium, is inoculated in the bacterium dish of diameter 5mm respectively on the AEA flat board that contains the variable concentrations gradient, cultivates down in 25 ℃ of dark conditions.Every processing repetition 3 times.When control strain colony diameter length is above to 6.5cm, adopt " right-angled intersection method " to measure.According to the linear regression analysis between the logarithm value of the probit value of inhibiting rate and medicine series concentration, obtain the EC that two kinds of single agent suppress mycelial growths 50Value, correlation coefficient and virulence regression equation.
On the basis of preliminary experiment, respectively the single agent bupirimate and the third oxygen quinoline are carried out toxicity test, EC in order to last method 50Value is followed successively by 4.89mg/l and 6.97mg/l.
The mixture toxicity test:
Carry out the toxicity test of mixture according to mixed ratio with single agent toxicity test method.
EC that each that will measure according to the abundant method of Sun Yun handled 50Value is converted into the actual toxicity index (ATI) of using with; According to the proportioning of mixture, obtain theory and use toxicity index (TTI) with, calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Single agent toxicity index=(standard medicament EC 50/ confession reagent agent EC 50) * 100
ATI=(standard medicament EC 50/ confession reagent agent (using with) EC 50) * 100
The content (%) of toxicity index * B in using with of content (the %)+B of toxicity index * A of TII=A in using with
CTC=(ATI/TTI)×100
If co-toxicity coefficient is greater than 120, showing has synergistic effect; If be starkly lower than 100 (below 80), be indicated as antagonism; Between 100~120, be indicated as summation action.
(4) experimental result and analysis:
The composite co-toxicity test of table 1 third oxygen quinoline and bupirimate to the powdery mildew of cucumber germ
The pharmacy quality ratio Virulence regression equation Correlation coefficient (r) EC 50(mg/L)
Bupirimate Y=3.2415X+2.7656 0.9741 4.89
The third oxygen quinoline Y=3.3006X+2.2168 0.9609 6.97
Bupirimate: the third oxygen quinoline is 3: 15 Y=3.1078X+3.3119 0.9577 3.49
Bupirimate: the third oxygen quinoline is 5: 13 Y=3.4211X+3.4259 0.9498 2.88
Bupirimate: the third oxygen quinoline is 7: 11 Y=3.2992X+3.3342 0.9536 3.20
Bupirimate: the third oxygen quinoline is 9: 9 Y=3.1755X+3.4695 0.9806 3.32
Bupirimate: the third oxygen quinoline is 11: 7 Y=3.3964X+3.2478 0.9724 3.28
The composite co-toxicity test of table 2 third oxygen quinoline and bupirimate to the powdery mildew of cucumber germ
Test medicine Mass ratio The actual measurement poison refers to Theoretical poison refers to Co-toxicity coefficient Conclusion
Bupirimate 1∶0 100.00 100.00 / /
The third oxygen quinoline 0∶1 70.16 70.16 / /
Bupirimate: the third oxygen quinoline 3∶15 140.00 75.13 186.34 Synergy
Bupirimate: the third oxygen quinoline 5∶13 169.52 78.45 216.09 Synergy
Bupirimate: the third oxygen quinoline 7∶11 152.91 81.76 187.01 Synergy
Bupirimate: the third oxygen quinoline 9∶9 147.48 85.08 173.35 Synergy
Bupirimate: the third oxygen quinoline 11∶7 149.09 88.39 168.66 Synergy
Measuring the result according to above indoor virulence test shows; The bupirimate and the third oxygen quinoline are by the mass percent experiment of experimental design; All show synergistic effect preferably; Wherein with bupirimate: the third oxygen quinoline is that 5: 13 o'clock co-toxicity coefficient is up to 216.09, and we can think that the bupirimate and the third oxygen quinoline percentage composition are that 5: 13 o'clock synergistic effects are best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 5: 13 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
Biologicall test instance 2: the composite Toxicity Determination of benzene oxygen quinoline and bupirimate to wheat powdery mildew.
The wheat powdery mildew germ, separation and purification gained from the wheat powdery mildew incidence of leaf of field is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
We carry out toxicity test, EC to single agent bupirimate and benzene oxygen quinoline 50Value is followed successively by 4.53mg/l and 7.76mg/l.
Combination setting is mixed:
20% bupirimate benzene oxygen quinoline percentage composition was respectively 4: 16,6: 14,8: 12,10: 10,12: 8, i.e. 4%+16%, 6%+14%, 8%+12%, 10%+10%, 12%+8% totally 5 combinations that are mixed.
Test method is similar with biologicall test embodiment 1.
Experimental result and analysis:
The composite virulence experiment of table 3 benzene oxygen quinoline and bupirimate to the wheat powdery mildew germ
The pharmacy quality ratio Virulence regression equation Correlation coefficient (r) EC 50(mg/L)
Bupirimate Y=2.8802X+3.1103 0.9835 4.53
Benzene oxygen quinoline Y=2.7941X+2.5136 0.9901 7.76
Bupirimate: benzene oxygen quinoline is 4: 16 Y=2.6635X+3.3748 0.9797 4.08
Bupirimate: benzene oxygen quinoline is 6: 14 Y=2.9014X+3.3684 0.9802 3.65
Bupirimate: benzene oxygen quinoline is 8: 12 Y=2.8246X+3.5996 0.9833 3.13
Bupirimate: benzene oxygen quinoline is 10: 10 Y=2.7715X+3.5417 0.9714 3.36
Bupirimate: benzene oxygen quinoline is 12: 8 Y=2.6433X+3.1677 0.9696 3.46
The composite co-toxicity test of table 4 benzene oxygen quinoline and bupirimate to the wheat powdery mildew germ
Figure BDA0000097893420000091
Measuring the result according to above indoor virulence test shows; In experiment under the percentage composition of used bupirimate and benzene oxygen quinoline; All show as synergistic effect; Wherein the co-toxicity coefficient when the percentage composition of bupirimate and benzene oxygen quinoline is 8: 12 is 192.81 to the maximum, thinks that promptly 8: 12 o'clock synergistic effects of percentage composition of bupirimate and benzene oxygen quinoline are best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 8: 12 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
Field trial 1: to the preventive effect of powdery mildew of cucumber
(1) investigation method
Experimental field be located at Shouguang, Shandong, publish the medium cucumber field of course of disease degree for experimental field, soil fertility is medium, and irrigation and drainage are convenient.Adopt the bilinear sampling method, 5 points are got in every sub-district, look into 5 caves at every, and 2 blades in the fixing middle part of every strain fix a point to check during investigation.
Come classification according to the powdery mildew of cucumber grade scale, write down total strain number, diseased plant number, degree of being in a bad way, calculate diseased plant rate, disease index and preventive effect.The powdery mildew of cucumber grade scale is:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%~10% of whole leaf area;
5 grades: lesion area accounts for 11%~25% of whole leaf area;
7 grades: lesion area accounts for 26%~50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
Test is investigated 3 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(2) control time and number of times
Test is investigated 3 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(3) drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
(4) poisoning investigation method
Whether continuous 7d range estimation medicament has poisoning to crop after the dispenser.
(5) experimental result and analysis
Table 5 is respectively organized the test medicine processing and is prevented and treated the test effect of powdery mildew of cucumber
Figure BDA0000097893420000101
Interpretation of result:
Can know that by table 5 effect that the combination that is mixed of 5% bupirimate+13% the third oxygen quinoline prevents and treats powdery mildew of cucumber very significantly.Under same 8,10 milliliters every mu consumption, the effect of complex preparation significantly is superior to two kinds of single agent, and its quick-acting and lasting effect are all relatively good, and is the most outstanding with lasting effect especially.Complex preparation significantly is superior to the effect under the low concentration consumption in control efficiency under the consumption of high concentration.20 days preventive effects reach 91.44% after 10 days best control efficiency behind the 1st, 2 medicine reaches 90.41% and 92.91%, the 2 medicine respectively.And single agent 20% the third oxygen quinoline EC; 10 days the highest preventive effect is 84.55% behind the 2nd medicine; 10 days preventive effects 86.35% behind the 2nd medicine of single agent 25% bupirimate SC; The effect of complex preparation significantly is superior to two kinds of single agent, has not only significantly improved the effect for the powdery mildew of cucumber germ, and has further prolonged its lasting period.
Field trial 2: to the preventive effect of wheat powdery mildew
(1) basic condition experimental field:
Experimental field be located at Henan, publish the medium wheat paddock of course of disease degree for experimental field, soil fertility is medium, and irrigation and drainage are convenient.Dispenser weather in March on the same day 31 is clear to cloudy, 11 ℃ of mean temperature of air, relative moisture 81%, 2~3 grades of southerlies; April 7 was fine, 18.2 ℃ of mean temperature of air, relative moisture 60%, 2~3 grades of southerlies.
(2) spraying time and method
At the 1st medicine of wheat powdery mildew their early stage (March 31) spray, (April 7) spray medicine second time sprays 2 times altogether behind the 7d.(operating pressure 0.2~0.3MPa), 750L carries out foliar spray according to the per hectare amount of liquid medicine to adopt the 3WBS-16 knapsack sprayer.
(3) pilot survey and data
Investigation state of an illness radix before the medicine, test is investigated 3 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(4) drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
(5) poisoning investigation method
Whether continuous 7d range estimation medicament has poisoning to crop after the dispenser.
(6) experimental result and analysis
Table 6 is respectively organized the test effect that test medicine is handled the control wheat powdery mildew
Figure BDA0000097893420000111
Figure BDA0000097893420000121
Interpretation of result:
Can know by table 6; The effect of the combination control wheat powdery mildew that is mixed of 8% bupirimate+12% benzene oxygen quinoline is more remarkable, and behind the medicine 10 days for the first time, 10 days and 20 days preventive effects 90.23%, 93.75% and 92.58% the highest behind the medicine for the second time; 10 days preventive effects the highest are 85.67% behind the 25% bupirimate SC medicine second time; 10 days preventive effect is up to 84.77% behind the 25% benzene oxygen quinoline SC medicine second time, and dosage is all greater than complex preparation, and the effect of complex preparation significantly is superior to two kinds of single agent; Not only significantly improved effect, and further prolonged its lasting period for wheat powdery mildew.
Comprehensive above experimental example is said; Of the present invention being that active ingredient is carried out two yuan of bactericidal composition preparations that are mixed Erysiphe, Puccinia, Rhynchosporium spp, nuclear cavity Pseudomonas and the microbial disease of Septoria are all had excellent preventive effect one of in bupirimate and the third oxygen quinoline or the benzene oxygen quinoline; Can effectively suppress further developing of disease; Simultaneously to crop safety; Suggestion is promoted the use of, and in use should be used alternatingly with other different mechanisms medicines, to delay the pesticide resistance of plant.

Claims (5)

1. pesticide sterilizing composite preparation; It is characterized in that: this bactericidal composition preparation is to be the preparation of two yuan of composite bactericidal compositions of active ingredient with bupirimate and the third oxygen quinoline or bupirimate and benzene oxygen quinoline; All the other components are agricultural chemicals used additives and formulation preparation complementary component in the preparation, wherein in this bactericidal composition in the preparation percentage by weight of two kinds of active ingredient bupirimates and the third oxygen quinoline or benzene oxygen quinoline be 90: 1~1: 90.
2. bactericidal composition preparation according to claim 1; It is characterized in that: on some specific aim ratio formulation screening test bases, the percentage by weight of the active ingredient bupirimate and the third oxygen quinoline or benzene oxygen quinoline is preferably 2~65 in this bactericidal composition preparation: 70~3.
3. bactericidal composition preparation according to claim 1 and 2; It is characterized in that: the gross mass of two kinds of active ingredient bupirimates and the third oxygen quinoline or benzene oxygen quinoline accounts for 1%~90% of total formulation gross mass in this bactericidal composition preparation, and all the other are agricultural chemicals used additives and formulation preparation complementary component.
4. bactericidal composition preparation according to claim 1 is characterized in that: the percentage that the gross mass of two kinds of active ingredient bupirimates and the third oxygen quinoline or benzene oxygen quinoline accounts for the total formulation gross mass in this bactericidal composition preparation is preferably 5%~70%.
5. according to the described bactericidal composition preparation of claim 1 to 4, it is characterized in that: the pesticidal preparations formulation that bactericidal composition preparation of the present invention can be mixed with is wetting powder, suspending agent, missible oil, soluble liquid, aqueous emulsion and water dispersible granules.
CN2011103066223A 2011-10-11 2011-10-11 Bupirimate-containing antibacterial composition Pending CN102349514A (en)

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CN103283737A (en) * 2013-06-25 2013-09-11 联保作物科技有限公司 Bactericidal composition and preparation thereof
CN103355309A (en) * 2013-07-25 2013-10-23 联保作物科技有限公司 Sterilization composition and preparation thereof
CN103444754A (en) * 2012-05-31 2013-12-18 陕西韦尔奇作物保护有限公司 Quinoxyfen-containing sterilization composition
CN104738047A (en) * 2015-03-19 2015-07-01 海利尔药业集团股份有限公司 Bactericidal composition containing Xinjunan acetate and bupirimate
CN106417303A (en) * 2016-12-05 2017-02-22 湖南泽丰农化有限公司 Proquinazid-containing high-efficiency bactericidal composition
CN106417317A (en) * 2016-12-01 2017-02-22 湖南泽丰农化有限公司 Application of proquinazid-containing bactericidal composition in controlling vegetable powdery mildew
CN106577675A (en) * 2016-12-02 2017-04-26 湖南泽丰农化有限公司 Sterilization composition containing proquinazid
CN106577673A (en) * 2016-12-02 2017-04-26 西安北农华农作物保护有限公司 Bactericidal composition containing Amisulbrom
CN112931513A (en) * 2021-02-08 2021-06-11 菏泽龙歌植保技术有限公司 Bactericide, preparation method and application thereof

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CN103444754A (en) * 2012-05-31 2013-12-18 陕西韦尔奇作物保护有限公司 Quinoxyfen-containing sterilization composition
CN103444754B (en) * 2012-05-31 2016-07-13 陕西韦尔奇作物保护有限公司 A kind of bactericidal composition of Quinoxyfen-containinsterilization
CN103283737A (en) * 2013-06-25 2013-09-11 联保作物科技有限公司 Bactericidal composition and preparation thereof
CN103355309A (en) * 2013-07-25 2013-10-23 联保作物科技有限公司 Sterilization composition and preparation thereof
CN103355309B (en) * 2013-07-25 2015-02-25 联保作物科技有限公司 Sterilization composition and preparation thereof
CN104738047A (en) * 2015-03-19 2015-07-01 海利尔药业集团股份有限公司 Bactericidal composition containing Xinjunan acetate and bupirimate
CN106417317A (en) * 2016-12-01 2017-02-22 湖南泽丰农化有限公司 Application of proquinazid-containing bactericidal composition in controlling vegetable powdery mildew
CN106577675A (en) * 2016-12-02 2017-04-26 湖南泽丰农化有限公司 Sterilization composition containing proquinazid
CN106577673A (en) * 2016-12-02 2017-04-26 西安北农华农作物保护有限公司 Bactericidal composition containing Amisulbrom
CN106417303A (en) * 2016-12-05 2017-02-22 湖南泽丰农化有限公司 Proquinazid-containing high-efficiency bactericidal composition
CN112931513A (en) * 2021-02-08 2021-06-11 菏泽龙歌植保技术有限公司 Bactericide, preparation method and application thereof

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Application publication date: 20120215