Summary of the invention
Object of the present invention is exactly to provide a kind of contain penconazole and composite bactericidal composition and the application thereof of Ningnanmycin for the defect of above-mentioned existence.Two yuan of composite bactericidal compositions that carry out as active ingredient taking penconazole and Ningnanmycin, for preventing and treating the fungal disease of agriculture vegetables, fruit tree, seeding corn and other crops, especially outstanding to downy mildew control efficiency, and can delay the drug-fast generation of germ, can expand fungicidal spectrum, reduce drug cost, improve actual preventive effect, delayed the generation of field drug-fastness, reduced peasant's drug cost, to environment environmental protection more, close friend, meet the requirement of agricultural production.
A kind of bactericidal composition that contains penconazole and Ningnanmycin of the present invention and application technology scheme thereof are, said composition contains penconazole and Ningnanmycin, the quality percentage composition of two kinds of composition sums of penconazole and Ningnanmycin in composition is 1%~90%, and all the other prepare complementary component for agricultural chemicals used additives and formulation.
Preferred penconazole and the quality percentage composition of two kinds of composition sums of Ningnanmycin in composition are 8%~70%, and all the other prepare complementary component for agricultural chemicals used additives and formulation.
Wherein, the mass ratio of penconazole and Ningnanmycin is 90: 1~1: 90.
Preferably, the mass ratio of penconazole and Ningnanmycin is 12: 1~1: 45.
The mass ratio of penconazole and Ningnanmycin is 12:1,4:1,3:2,1:1,2:3,1:4,3:7,2:23 or 1:45.
Said composition can be wetting powder, suspending agent, soluble liquid, missible oil, aqueous emulsion or water dispersible granules;
Wherein, bactericidal composition is wetting powder, comprises the component of following mass percent: penconazole 1 ~ 90%, Ningnanmycin 1 ~ 90%, dispersant 3 ~ 20%, wetting agent 3 ~ 10%, filler 1 ~ 70%; By above-mentioned formula, by penconazole and Ningnanmycin and dispersant, wetting agent and filler mixing, uniform stirring in stirred tank is mixing after airslide disintegrating mill, makes wetting powder;
Bactericidal composition is suspending agent, comprises the component of following mass percent: penconazole 1 ~ 90%, and Ningnanmycin 1 ~ 90%, dispersant 5~20%, antifreezing agent 1~5%, thickener 0.1~2%, defoamer 0.1~0.8%, surplus is water; First other auxiliary agents are mixed, mix through high speed shear, add penconazole and Ningnanmycin, abrading-ball 2 ~ 3 hours in ball crusher, makes a diameter all below 5mm, makes suspending agent preparation;
Bactericidal composition is aqueous emulsion, comprises the component of following mass percent: penconazole 1 ~ 90%, and Ningnanmycin 1 ~ 90%, emulsifier 3~30%, solvent 5~15%, stabilizing agent 2~15%, antifreezing agent 1~5%, defoamer 0.1~8%, thickener 0.2~2%, surplus is water; Penconazole and Ningnanmycin, solvent, emulsifier and thickener are added together, make to be dissolved into uniform oil phase; By part water, antifreezing agent, stabilizing agent, defoamer mix into uniform water; When reactor high speed stirs, oil phase is added to water, slowly add water until reach phase inversion point, open clipper and carry out high speed shear, and add remaining water, shears 20 minutes-1 hour, the aqueous emulsion of formation oil-in-water type;
Bactericidal composition is water dispersible granules, comprises the component of following mass percent: penconazole 1 ~ 90%, Ningnanmycin 1 ~ 90%, dispersant 3 ~ 20%, wetting agent 3 ~ 10%, disintegrant 2 ~ 5%, filler 1 ~ 70%; Penconazole, Ningnanmycin and dispersant, wetting agent, disintegrant and filler are mixed, pulverize with micro jet, through mediate, then add in fluidized bed prilling dryer, carry out granulation, dry, screening after through sample analysis, make the water dispersible granules of bactericidal composition;
Or bactericidal composition is missible oil, comprise the component of following mass percent: penconazole 1 ~ 90%, Ningnanmycin 1 ~ 90%, emulsifier 1 ~ 15%, solvent complementary component; According to formula rate, penconazole and Ningnanmycin are dissolved in solvent, add emulsifier, be uniformly mixed and make said composition cream preparation.
Emulsifier is at least one in calcium dodecyl benzene sulfonate and aliphatic acid polyethenoxy ether, alkylphenol polyoxyethylene sulfosuccinate, styrylphenol polyoxyethylene ether, polyoxyethylene nonylphenol ether, castor oil polyoxyethylene ether, aliphatic acid polyethenoxy base ester, polyoxyethylene aliphatic alcohol ether;
Described solvent is at least one in dimethylbenzene or biodiesel, toluene, diesel oil, methyl alcohol, ethanol, n-butanol, isopropyl alcohol, turpentine oil, solvent naphtha, dimethyl formamide, dimethyl sulfoxide (DMSO), water;
Described dispersant is at least one in polycarboxylate, lignosulfonates, alkylphenol polyoxyethylene formaldehyde condensation products sulphate, alkyl benzene sulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol polyoxyethylene, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, fatty acid glyceride APEO;
Described wetting agent is lauryl sodium sulfate, calcium dodecyl benzene sulfonate, pull open at least one in powder Ningnanmycin X., wetting and penetrating agent F, alkylnaphthalene sulfonate, polyoxyethylene triphenylethylene phosphenylic acid salt, spaonin powder, silkworm excrement, soapberry powder;
Described disintegrant is at least one in bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, sodium bicarbonate;
Described thickener is at least one in xanthans, CMC, hydroxyethylcellulose, methylcellulose, Magnesiumaluminumsilicate, polyvinyl alcohol;
Described stabilizing agent is at least one in sodium citrate, resorcinol;
Described antifreezing agent is at least one in ethylene glycol, propane diols, glycerine;
Described defoamer is at least one in silicone oil, silicone compound, C10-20 saturated fat acid compounds, C8-10 aliphatic alcohols compound;
Described filler is at least one in kaolin, diatomite, bentonite, attapulgite, white carbon, starch, precipitated calcium carbonate.
The application of described bactericidal composition, acts on the mould Pseudomonas of Erysiphe, Rust, spore, Septoria, Venturia germ on cereal crop, coffee, beet, fruit tree and grape; Prevent and treat cereal and coffee rust, cereal, fruit tree and vegetables powdery mildew, peanut, beet tops pinta, apple scab and peanut white rot; Downy mildew, epidemic disease, anthracnose, canker, fusarium wilt, leaf spot, the angular leaf spot of control fruit tree, vegetables, cotton and grain and oil crop.
Beneficial effect of the present invention is: bactericidal composition of the present invention is compared similar medicament many advantages, first, two yuan of composite bactericidal compositions that carry out as active ingredient taking penconazole and Ningnanmycin, compare single component and have synergistic effect, can expand fungicidal spectrum, greatly improve the preventive effect to controlling fungal diseases of crop, be used for preventing and treating agriculture vegetables, fruit tree, the fungal disease of seeding corn and other crops, especially to downy mildew, epidemic disease, powdery mildew has special efficacy, second, drug effect is more lasting, and be difficult for by rain drop erosion, quick-acting is compared single dose also very big raising, the 3rd, greatly delay drug-fast generation, reduce agricultural chemicals Reusability number of times, reduce agricultural chemicals usage amount, reduce peasant's use cost, the 4th, low-residual, lasting period easily degraded in soil later, consumption is few, more friendly to environment, meet the requirement of agricultural production.
embodiment:
In order to understand better the present invention, describe technical scheme of the present invention in detail with instantiation below, but the present invention is not limited thereto.
The method that the present invention adopts indoor bioassay and field trial to combine.If no special instructions, the ratio (comprising percentage) of below mentioning is all mass ratio.
The following stated emulsifier is at least one in calcium dodecyl benzene sulfonate and aliphatic acid polyethenoxy ether, alkylphenol polyoxyethylene sulfosuccinate, styrylphenol polyoxyethylene ether, polyoxyethylene nonylphenol ether, castor oil polyoxyethylene ether, aliphatic acid polyethenoxy base ester, polyoxyethylene aliphatic alcohol ether;
Described solvent is at least one in dimethylbenzene or biodiesel, toluene, diesel oil, methyl alcohol, ethanol, n-butanol, isopropyl alcohol, turpentine oil, solvent naphtha, dimethyl formamide, dimethyl sulfoxide (DMSO), water;
Described dispersant is at least one in polycarboxylate, lignosulfonates, alkylphenol polyoxyethylene formaldehyde condensation products sulphate, alkyl benzene sulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol polyoxyethylene, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, fatty acid glyceride APEO;
Described wetting agent is lauryl sodium sulfate, calcium dodecyl benzene sulfonate, pull open at least one in powder Ningnanmycin X., wetting and penetrating agent F, alkylnaphthalene sulfonate, polyoxyethylene triphenylethylene phosphenylic acid salt, spaonin powder, silkworm excrement, soapberry powder;
Described disintegrant is at least one in bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, sodium bicarbonate;
Described thickener is at least one in xanthans, CMC, hydroxyethylcellulose, methylcellulose, Magnesiumaluminumsilicate, polyvinyl alcohol;
Described stabilizing agent is at least one in sodium citrate, resorcinol;
Described antifreezing agent is at least one in ethylene glycol, propane diols, glycerine;
Described defoamer is at least one in silicone oil, silicone compound, C10-20 saturated fat acid compounds, C8-10 aliphatic alcohols compound;
Described filler is at least one in kaolin, diatomite, bentonite, attapulgite, white carbon, starch, precipitated calcium carbonate.
Embodiment 1
Penconazole and Ningnanmycin suspending agent
Penconazole 6g, Ningnanmycin 14g, lignosulfonates 14.6g, propane diols 4.8g, xanthans 2g, silicone oil 0.8g, surplus is that water complements to 100g, other auxiliary agents are mixed, mix through high speed shear, add penconazole and Ningnanmycin, abrading-ball 2 ~ 3 hours in ball crusher, make a diameter all below 5mm, make the suspending agent preparation of the bactericidal composition that contains penconazole and Ningnanmycin of the present invention.
Embodiment 2
Penconazole and Ningnanmycin suspending agent
Penconazole 8g, Ningnanmycin 12g, lignosulfonates 5g, propane diols 2.4g, xanthans 1.1g, silicone oil 0.63g, water surplus complements to 100g, other auxiliary agents are mixed, mix through high speed shear, add penconazole and Ningnanmycin, abrading-ball 2 ~ 3 hours in ball crusher, make a diameter all below 5mm, make the suspending agent preparation of the bactericidal composition that contains penconazole and Ningnanmycin of the present invention.
Embodiment 3
Penconazole and azoles alcohol aqueous emulsion
Penconazole 6g, Ningnanmycin 14g, castor oil polyoxyethylene ether 5, dimethylbenzene 14.5g, propane diols 1g, silicone oil 0.2g, water complements to 100g.Former medicine penconazole and Ningnanmycin, solvent and emulsifier are added together, make to be dissolved into uniform oil phase; By part water, other the insecticides adjuvant such as antifreezing agent mixes into uniform water; When reactor high speed stirs, oil phase is added to water, slowly add water until reach phase inversion point, open clipper and carry out high speed shear, and add remaining water, shear about half an hour, form the aqueous emulsion of oil-in-water type.Make the aqueous emulsion of the bactericidal composition that contains penconazole and Ningnanmycin.
Embodiment 4
Penconazole and Ningnanmycin water dispersible granules
Penconazole 12g, Ningnanmycin 8g, lignosulfonates 12.7g, lauryl sodium sulfate 3.51g, ammonium sulfate 2.66g, diatomite is supplied 100g.Penconazole, Ningnanmycin and dispersant, wetting agent, disintegrant and filler are mixed, pulverize with micro jet, through mediating, then add and in fluidized bed prilling dryer, carry out after granulation, dry, screening, through sample analysis, making the water dispersible granules of the bactericidal composition that contains penconazole and Ningnanmycin of the present invention.
Embodiment 5
Penconazole and Ningnanmycin missible oil
Penconazole 14g, Ningnanmycin 6g, emulsifier castor oil polyoxyethylene ether 6g, calcium dodecyl benzene sulfonate 5g, dimethylbenzene is supplied 100g.According to formula rate, enough penconazoles and Ningnanmycin are dissolved in solvent, add emulsifier, be uniformly mixed and make said composition cream preparation.
Embodiment 6
Penconazole and Ningnanmycin wetting powder
The percentage by weight of each component is: penconazole 60%, Ningnanmycin 5%, dispersant 6%, wetting agent 6%, filler 23%;
Above dispersant is: polycarboxylate;
Wetting agent is: lauryl sodium sulfate;
Filler is: kaolin.
By above-mentioned formula, by penconazole and Ningnanmycin and dispersant, wetting agent and filler mixing, uniform stirring in stirred tank is mixing after airslide disintegrating mill, makes wetting powder.
Embodiment 7
Penconazole and Ningnanmycin suspending agent
The percentage by weight of each component is: penconazole 10%, Ningnanmycin 10%, dispersant 10%, antifreezing agent 4%, thickener 1%, defoamer 0.4%, and surplus is water;
Dispersant is: alkylphenol polyoxyethylene;
Antifreezing agent is: ethylene glycol;
Thickener is: xanthans;
Defoamer is: silicone oil;
The concrete production stage of this suspending agent is: first auxiliary agent is mixed by above-mentioned proportioning, mix through high speed shear, add penconazole and Ningnanmycin, abrading-ball 2~3 hours in ball crusher, make a diameter all below 5 mm, obtain the bactericidal composition of suspending agent preparation.
Embodiment 8
Penconazole and Ningnanmycin aqueous emulsion
The percentage by weight of each component is: penconazole 2%, Ningnanmycin 23%, emulsifier 10%, solvent 10%, stabilizing agent 3%, antifreezing agent 3%, defoamer 2%, thickener 1%, surplus are water;
Emulsifier is: styrylphenol polyoxyethylene ether;
Solvent is: dimethylbenzene;
Stabilizing agent is: sodium citrate;
Antifreezing agent is: ethylene glycol;
Defoamer is: silicone oil;
Thickener is: CMC;
The concrete production stage of this aqueous emulsion is: penconazole and Ningnanmycin, solvent, emulsifier and thickener are added together, make to be dissolved into uniform oil phase; By part water, antifreezing agent, stabilizing agent, defoamer mix into uniform water; When reactor high speed stirs, oil phase is added to water, slowly add water until reach phase inversion point, open clipper and carry out high speed shear, and add remaining water, shears 20 minutes-1 hour, the aqueous emulsion of formation oil-in-water type.
Embodiment 9
Penconazole and Ningnanmycin water dispersible granules
The mass percent of each component is: penconazole 1%, Ningnanmycin 45%, dispersant 15%, wetting agent 8%, disintegrant 3%, filler 28%;
Dispersant is: fatty acid glyceride APEO;
Wetting agent: polyoxyethylene triphenylethylene phosphenylic acid salt;
Disintegrant is: citric acid;
Filler is: bentonite;
The concrete production stage of this aqueous emulsion is: penconazole, Ningnanmycin and dispersant, wetting agent, disintegrant and filler are mixed, pulverize with micro jet, through mediating, then add in fluidized bed prilling dryer and carry out granulation, dry, screening, obtain the bactericidal composition of water dispersant;
Embodiment 10
Penconazole and Ningnanmycin missible oil
The mass percent of each component is: penconazole 1%, and Ningnanmycin 45%, emulsifier 8%, surplus is solvent;
Emulsifier is: polyoxyethylene nonylphenol ether;
Solvent is: dimethyl sulfoxide (DMSO);
The concrete production stage of this missible oil is: according to formula rate, penconazole and Ningnanmycin are dissolved in solvent, add emulsifier, be uniformly mixed and make said composition cream preparation.
Embodiment 11
Be below indoor virulence and field experiment
The synergy test that penconazole and Ningnanmycin are composite.It is tested object that test adopts bacterium of downy mildew of cucumber.
Concrete grammar is:
(1) test medicine
The former medicine of 95% penconazole, Hangzhou space dragon, the former medicine of 95% Ningnanmycin, Deqiang Biology Co., Ltd..
Penconazole and Ningnanmycin dissolve by proper amount of acetone, are made into the mother liquor of 10000 μ g/ml, and at 4 DEG C, low temperature is preserved, stand-by, before test, are diluted to a series of concentration with sterile water.
(2) subjects
Cucumber downy mildew germ, separation and purification gained from the Vegetable Base greenhouse cucumber incidence of leaf of Yi Feng shop, Shandong is cultivated and is preserved in 4 DEG C of refrigerators on PDA inclined-plane.
PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml.
AEA medium: dusty yeast 5g/L, glycerine 20mL/L, MgSO
40.25g/L, NaNO
36g/L, KCl0.5g/L, KH
2pO
41.5g/L, agar powder 20g/L, deionized water 1L.
(3) test method
Bacterium of downy mildew of cucumber after preculture, is inoculated in the bacterium dish of diameter 5mm respectively on the AEA flat board containing variable concentrations gradient on AEA medium, under 25 DEG C of dark conditions, cultivates.Every processing repeats 3 times.When control strain colony diameter grows to 6.5cm when above, adopt " right-angled intersection method " to measure.According to the linear regression analysis between the probit value of inhibiting rate and the logarithm value of medicine series concentration, obtain the EC that two kinds of single doses suppress mycelial growth
50value, correlation coefficient and virulence regression equation.
On the basis of preliminary experiment, respectively single dose penconazole and Ningnanmycin are carried out to toxicity test in order to upper method, the two EC
50value is respectively 5.13 ㎎/L and 4.69 ㎎/L.。
Test 20% penconazole Ningnanmycin mass ratio and be respectively 4:16,6:14,8:12,10:10,12:8,14:6, totally six mixture combinations.
(4) data statistics and analysis
Carry out the toxicity test of mixture according to mixed ratio by single dose toxicity test method.
According to the abundant method of Sun Yun by the EC of each processing measuring
50value is converted into actual mixed toxicity index (ATI); According to the proportioning of mixture, obtain theoretical mixed toxicity index (TTI), calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Single dose toxicity index=(standard medicament EC
50/ reagent agent EC
50) × 100
ATI=(standard medicament EC
50/ reagent agent (using with) EC
50) × 100
The content (%) of toxicity index × B of content (the %)+B of toxicity index × A of TII=A in mixed in mixed
CTC=(ATI/?TTI)×100
If co-toxicity coefficient is large 120, showing has synergistic effect; If be starkly lower than 100 (below 80), be indicated as antagonism; Between 100~120, be indicated as summation action.
(5) toxicity test result and analysis
Virulence and the synergistic effect of the different proportioning penconazole of table 1 Ningnanmycin mixture to bacterium of downy mildew of cucumber
。
The composite co-toxicity to bacterium of downy mildew of cucumber of table 2 penconazole Ningnanmycin
。
According to bactericide joint toxicity measuring result 20% penconazole Ningnanmycin suspending agent, co-toxicity coefficient when 4:16,6:14,8:12,10:10,12:8,14:6 all obviously large 120, all show as synergistic effect, especially in the time that penconazole and Ningnanmycin mass ratio are 6:14, co-toxicity coefficient is up to 167.45, and we think that its synergistic effect is best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 6: 14 left and right scopes of suitable proportion, to evaluate its field practical application effect.
Embodiment 12
Field trial is measured the control efficiency to cucumber downy mildew
(1) test method
Test dispenser twice altogether, check plot spray equivalent clear water.While making up a prescription, after first medicament fully being dissolved with a small amount of water, then add suitable quantity of water to carry out complete stool spraying processing, even spraying.When spray medicine, all adopting power driven sprayer, operating pressure is 0.3~0.4MPa, shower nozzle aperture 1.3mm.Community spouting liquid is 40 ~ 50kg/667m
2, dispenser twice continuously, 10 days intervals, sprays application to crop complete stool uniformly by liquid.
(2) investigation method
Experimental field be located at Qingdao Jimo City, select the medium green house of occurring degree for experimental field, soil fertility is medium, and irrigation and drainage are convenient.Adopt bilinear sampling method, 5 points are got in every community, look into 5 caves at every, and when investigation, 2 blades in the fixing middle part of every strain fix a point to check.
Carry out classification according to cucumber downy mildew grade scale, record total strain number, diseased plant number, severity, calculate diseased plant rate, disease index and preventive effect.
Cucumber downy mildew grade scale is
0 grade: without scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6% ~ 10% of whole leaf area;
5 grades: lesion area accounts for 11% ~ 25% of whole leaf area;
7 grades: lesion area accounts for 26% ~ 50% of whole leaf area;
9 grades: lesion area accounts for the more than 50% of whole leaf area.
Test is investigated 3 times altogether, i.e. disease index investigation before medicine, preventive effect investigation in 10 days after the 1st medicine, 10 days and preventive effect investigation in 20 days after 2 medicines.
(3) poisoning investigation
After dispenser, whether 7d range estimation medicament has poisoning to crop continuously.
(4) drug effect computational methods
The total number of sheets × 100 of sick leaf rate (%)=sick number of sheets/investigate
Disease index=∑ (the sick number of sheets × relative level numerical value at different levels)/(investigating the total number of sheets × 9) × 100
Control efficiency (%)=(disease index after the front disease index × treatment region medicine of 1-(blank district medicine)/(the front disease index of disease index × treatment region medicine after blank district medicine)) × 100
(5) field control effectiveness test result of the test
Be disease index investigation before medicine, preventive effect investigation in 10 days after the 1st medicine, 10 days and preventive effect investigation in 20 days after 2 medicines.
(6) field control effectiveness test result of the test
The each effect of processing control cucumber downy mildew of table 3
。
Table 3 can find out, the effect of the mixture combination control cucumber downy mildew of 20% penconazole Ningnanmycin 6:14 is very remarkable.Evidence complex preparation control efficiency under the consumption of high concentration is significantly better than the effect under low concentration consumption.Under the consumption of 20 grams every mu, after the 2nd medicine, the highest preventive effect of 20 days can reach 84% left and right.And the preventive effect of penconazole and Ningnanmycin single dose respectively in 77% and 79% left and right significantly inferior to complex preparation.The quick-acting of complex preparation and lasting effect are all more outstanding, are the medicament of desirable control cucumber downy mildew.Duration of test is observed, and it,, to cucumber safety, does not produce harmful effect.
Embodiment 13
Field trial is measured the control efficiency to Pumpkin powdery mildew
(1) test method
Test dispenser twice altogether, check plot spray equivalent clear water.While making up a prescription, after first medicament fully being dissolved with a small amount of water, then add suitable quantity of water to carry out complete stool spraying processing, even spraying.When spray medicine, all adopting power driven sprayer, operating pressure is 0.3~0.4MPa, shower nozzle aperture 1.3mm.Community spouting liquid is 40 ~ 50kg/667m
2, dispenser twice continuously, 10 days intervals, sprays application to crop complete stool uniformly by liquid.
(2) investigation method
Experimental field be located at Qingdao Jimo City, select the medium green house of occurring degree for experimental field, soil fertility is medium, and irrigation and drainage are convenient.Adopt bilinear sampling method, 5 points are got in every community, look into 5 caves at every, and when investigation, 2 blades in the fixing middle part of every strain fix a point to check.
Carry out classification according to Pumpkin powdery mildew grade scale, record total strain number, diseased plant number, severity, calculate diseased plant rate, disease index and preventive effect.
Pumpkin powdery mildew grade scale is
0 grade: without scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6% ~ 10% of whole leaf area;
5 grades: lesion area accounts for 11% ~ 25% of whole leaf area;
7 grades: lesion area accounts for 26% ~ 50% of whole leaf area;
9 grades: lesion area accounts for the more than 50% of whole leaf area.
Test is investigated 3 times altogether, i.e. disease index investigation before medicine, preventive effect investigation in 10 days after the 1st medicine, 10 days and preventive effect investigation in 20 days after 2 medicines.
(3) poisoning investigation
After dispenser, whether 7d range estimation medicament has poisoning to crop continuously.
(4) drug effect computational methods
The total number of sheets × 100 of sick leaf rate (%)=sick number of sheets/investigate
Disease index=∑ (the sick number of sheets × relative level numerical value at different levels)/(investigating the total number of sheets × 9) × 100
Control efficiency (%)=(disease index after the front disease index × treatment region medicine of 1-(blank district medicine)/(the front disease index of disease index × treatment region medicine after blank district medicine)) × 100
(5) field control effectiveness test result of the test
Be disease index investigation before medicine, preventive effect investigation in 10 days after the 1st medicine, 10 days and preventive effect investigation in 20 days after 2 medicines.
(6) field control effectiveness test result of the test
The each effect of processing control Pumpkin powdery mildew of table 4
。
Table 4 can find out, the effect of the mixture combination control Pumpkin powdery mildew of 20% penconazole Ningnanmycin 6:14 is very remarkable.Evidence complex preparation control efficiency under the consumption of high concentration is significantly better than the effect under low concentration consumption.Under the consumption of 20 grams every mu, after the 2nd medicine, the highest preventive effect of 20 days can reach 85% left and right.And the preventive effect of penconazole and Ningnanmycin single dose is respectively in 79% and 74% left and right, significantly inferior to complex preparation.The quick-acting of complex preparation and lasting effect are all more outstanding, are the medicament of desirable control Pumpkin powdery mildew.Duration of test is observed, and it,, to custard squash safety, does not produce harmful effect.
In sum, the Bactericidal composite that the present invention contains penconazole and Ningnanmycin, in control, cereal, fruit tree and vegetables powdery mildew, peanut, beet tops pinta, all there is good control efficiency downy mildew, epidemic disease, the anthracnose aspect of control fruit tree, vegetables, cotton and grain and oil crop, especially outstanding to the control efficiency of downy mildew and powdery mildew, and it is to target crop safety.Compared with single dose, it is long that bactericidal composition of the present invention has the lasting period, easy to use, is difficult for producing the advantage of poisoning.By composite, improve bactericidal activity, expand fungicidal spectrum, widen the scope of application, reduce costs, multiple diseases is played to the double effect of controlling of a medicine, alleviate manpower and materials, improve productivity effect.So the invention of this complex preparation is of great significance social tool with popularization.