CN102273475A - High efficiency germicide composition - Google Patents
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- CN102273475A CN102273475A CN2011101734494A CN201110173449A CN102273475A CN 102273475 A CN102273475 A CN 102273475A CN 2011101734494 A CN2011101734494 A CN 2011101734494A CN 201110173449 A CN201110173449 A CN 201110173449A CN 102273475 A CN102273475 A CN 102273475A
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Abstract
The invention relates to a germicide composition, which belongs to the field of compounding of pesticides. The germicide composition is prepared from binary mixing of active components of the composition, i.e., bixafen and a part of dithiocarbamate bactericides, wherein, the weight ratio of the two active components is 80:1 to 1:90, preferably, 50-1:5-70; total weight percentage of the active components in the germicide composition is 1% to 90%, preferably, 5% to 75%, the rest being auxiliary components allowed to use and acceptable in pesticides; the germicide composition can be made into wettable pulvis, a suspending agent, a dry suspending agent, a soluble liquid formulation and water dispersible granules by using known methods. The germicide composition provided in the invention is mainly used for controlling fungal diseases and bacterial diseases of field crops, e.g., vegetable, fruit trees, corn, paddy rice and wheat.
Description
Technical field
The present invention relates to the agriculture chemical compounding technical field, be specifically related to a kind of with new type bactericide bixafen (C
18H
12Cl
2F
3N
3O) with in part dithiocarbamates compound such as mancozeb, tmtd, zineb, Carbatene, the ziram one of any carry out two yuan of bactericidal compositions that are mixed for active ingredient.
Background technology
Bixafen (C
18H
12Cl
2F
3N
3O), be a kind of novel SDI pyrazoles bactericide, the chemical structural formula of bixafen is:
Bixafen is the succinate dehydrogenase inhibitor, the control that is used for leaf spot and leaf rust will mainly be developed, but by studies show that, this new compound all has excellent activity to multiple germ, as the comparatively general germ of pesticide resistance such as white powder germ, ash arrhizus bacteria, Pseudoperonospora cubensis, be expected to become the important kind of bactericide resistance management.
Mancozeb is a kind of good protective fungicide, belongs to low-toxin farm chemicals.Because wide, the difficult resistance that produces of its bactericidal range, control efficiency obviously is better than other same series bactericidal agents, manganese, zinc microelement have significantly short strong, production-increasing function to crop, remarkable to preventing and treating effects such as pear scab, alternaria leaf spot of apple, vegetable category eqpidemic disease, downy mildew, field crop rust
Tmtd, good fortune U.S.A of a kind of broad spectrum protection is a bactericide, to various crop downy mildew, eqpidemic disease, anthracnose, cereal class smut, seedling stage yellow blight better prevention is arranged.Seed transmission and the traditional disease of soil in seedling stage there is lethal effect preferably.
Ziram, the chemical name zine dimethyldithiocarbamate, on the agricultural as the sterilization propellant in orchard.Can prevent and treat apple rust, shot hole, downy mildew of garpe, celery eqpidemic disease and spinach downy mildew etc.
Zineb is the protectiveness organophosphorus fungicide, the formal name used at school zinc ethylene bisdithiocarbamate, be mainly used in the blade face protection, can prevent and treat multiple fungus-caused disease, be broad-spectrum germicide, can prevent and treat the multiple diseases of paddy rice, fruit tree, vegetables and tobacco, as tomato blight, anthracnose, ring spot; Brassicaceous vegetable and beans soft rot, anthracnose, soybean rust; The scab of pears, apple; Anthracnose of tobacco, rust; Wheat rust; Rice sheath blight disease etc.
Propineb is a kind of wide spectrum, quick-acting protective fungicide; be applicable to Chinese cabbage downy mildew, cucumber downy mildew, the tomato sick control such as late blight, mango anthracnose early of tomato, Chinese cabbage, cucumber, mango and flowers and other crops; downy mildew of crops such as vegetables, tobacco, lupulus and morning, the late blight of tomato and potato all there are good protective action, and the germ disease of powdery mildew, rust and Botrytis is also had certain inhibitory action.
Carbatene (metiram) chemical name: the co-ordination complex of two two sulphur deoxidation base formic acid manganese of ethylene and zinc ion, be protective fungicide, the fungus-caused various diseases of Oomycete there is good preventive effect.
Various places climate variability in recent years, relatively do not have rule, corps diseases is comparatively serious, and traditional bactericide such as dithiocarbamate fungicide, the long-term use, caused the crops pesticide resistance to produce, dosage increases, and the lasting period shortens, preventive effect reduces, the repeated drug taking increased frequency increases agricultural cost, and is unfavorable for the sustainable development of environment.I research staff of company attempts with the new type bactericide of the different mechanisms of action composite with it, to overcome the above problems.Prescription screening through a large amount of indoor biometrics tests and field experiment, chance on, carry out two yuan for active ingredient and be mixed one of any in new type bactericide bixafen and the above part dithiocarbamates compound such as mancozeb, tmtd, zineb, Carbatene, the ziram, synergistic effect is comparatively obvious, can obviously improve diseases control, reduce dosage, also can enlarge fungicidal spectrum, improve germicidal efficiency.And, do not see relevant open report at present at home and abroad as yet about the research in new type bactericide bixafen and the composite field of above-mentioned dithiocarbamates compound.
Summary of the invention
I research staff of company is devoted for years in the research in the composite field of agriculture chemicals fungicide, to utilize comparatively limited development resources and ability, make valuable innovation in the bactericide field, through a large amount of formula test screenings, a kind of bactericidal composition has efficiently been proposed, it is bactericidal composition of the present invention, bactericidal composition of the present invention is to be that two yuan of carrying out of active ingredient are mixed with new type bactericide bixafen and part dithiocarbamate fungicide, be used to prevent and treat paddy rice, fruit tree, vegetables, tobacco, brassicaceous vegetable and beans, the disease of wheat class, can obviously delay the drug-fast generation of germ, enlarge fungicidal spectrum, reduce drug cost, improve actual preventive effect.
Specifically, the present invention for a kind of be two yuan of bactericidal composition preparations that are mixed that active ingredient is carried out with new type bactericide bixafen and part dithiocarbamate fungicide, the percentage by weight of active ingredient new type bactericide bixafen and dithiocarbamate fungicide is 80～1: 1～90 in the bactericidal composition wherein of the present invention, in concrete test recipe screening, the percentage by weight of active ingredient bixafen and dithiocarbamate fungicide can be preferably 50～1: 5～70.
Dithiocarbamates compound of the present invention is specially in mancozeb, zineb, Carbatene, tmtd, the ziram one of any.
Pesticide sterilizing composite of the present invention is wetting powder, suspending agent, dry suspending agent, soluble liquid and water dispersible granules with the formulations of pesticide that the preparation method in pesticidal preparations field can be prepared into, the gross mass percentage composition of active ingredient is 1%～90% in the preparation, be preferably 5%～75%, all the other are usual auxiliaries in the agricultural chemicals.
Pesticide sterilizing composite of the present invention all has the better prevention effect to the diseases such as leaf spot, powdery mildew, rust, downy mildew, eqpidemic disease, anthracnose and rice blast of paddy rice, fruit tree, vegetables, for example prevents and treats the ring spot of downy mildew of garpe, melon anthracnose and downy mildew, rice sheath blight disease and rice blast, pear scab, alternaria leaf spot of apple, vegetable category eqpidemic disease, field crop rust, cereal class smut, seedling stage yellow blight, fruit tree; Brassicaceous vegetable and beans soft rot, soybean rust; Anthracnose of tobacco, rust; The control that mango anthracnose etc. are sick also has certain inhibitory action to the germ disease of Botrytis, and the fungus-caused various diseases of Oomycete are had good preventive effect.
In addition, bactericidal composition of the present invention is compared similar medicament a lot of obviously advantages, at first can improve the preventive effect to corps diseases greatly, and quick-acting is compared single agent and improved a lot, and drug effect is also more lasting; Secondly delay the drug-fast generation of crop greatly, reduced agricultural chemicals access times repeatedly, reduced the usage amount of agricultural chemicals, reduced peasant's use cost; The residual quantity of this bactericidal composition is lower once more, and the lasting period decomposes than being easier to later, and the usage amount of agricultural chemicals is less relatively, and is also friendly more to environment; In the application of this bactericidal composition crops there is general production-increasing function at last, the increasing both production and income of agricultural region is had bigger meaning.
Embodiment:
In order to make purpose of the present invention, technical scheme and advantage clearer, the present invention describes with following specific embodiment, but the present invention only limits to these examples absolutely not.The method that the present invention adopts indoor bioassay and field trial to combine.If no special instructions, the ratio of below mentioning (comprising percentage) all is a mass ratio.Should be pointed out that all any modifications of being made within the spirit and principles in the present invention, be equal to replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Wherein prepare required necessary basis component about the formulations of pesticide and the easy manufacture implementation method as follows:
Bixafen (C in following implementer's case
18H
12Cl
2F
3N
3O) represent with active principle A that active principle B represents in part dithiocarbamates compound mancozeb, Carbatene, zineb, tmtd, the ziram one of any.
One of technical scheme of the present invention, described bactericidal composition are wetting powder, and the percentage by weight of component is:
The concrete procedure of processing of this bactericidal composition wetting powder is: press above-mentioned prescription with active principle A and active principle B and dispersant, wetting agent and filler mixing, in stirred tank, evenly stir, behind airslide disintegrating mill, mixing, promptly can be made into the wetting powder of the present composition.
Two of technical scheme of the present invention, described bactericidal composition are suspending agent, and the percentage by weight of component is:
The concrete production stage of this suspending agent is earlier other auxiliary agents to be mixed, mix through high speed shear, add active principle A and active principle B, abrading-ball is 2～3 hours in ball crusher, make a diameter all below 5mm, make the suspending agent preparation of bactericidal composition of the present invention.
Three of technical scheme of the present invention, described bactericidal composition are water dispersible granules, and the percentage by weight of component is:
The concrete procedure of processing of this bactericidal composition water dispersible granules is: by above-mentioned prescription active principle A, active principle B and dispersant, wetting agent, disintegrant and filler are mixed, pulverize with micro jet, through mediating, add then and carry out granulation, drying, screening in the fluidized bed prilling dryer after sample analysis, promptly make the water dispersible granules of bactericidal composition of the present invention.
Described emulsifier is selected from any one or more mixtures formed with arbitrary proportion in calcium dodecyl benzene sulfonate and aliphatic acid polyethenoxy ether, alkylphenol polyoxyethylene sulfosuccinate, styryl phenol APEO, polyoxyethylene nonylphenol ether, castor oil polyoxyethylene ether, aliphatic acid polyethenoxy base ester, the polyoxyethylene aliphatic alcohol ether.
Described solvent is any one or more mixed solvents formed with arbitrary proportion in dimethylbenzene or biodiesel, toluene, diesel oil, methyl alcohol, ethanol, n-butanol, isopropyl alcohol, turpentine oil, solvent naphtha, dimethyl formamide, dimethyl sulfoxide (DMSO), the water equal solvent.
Described dispersant is selected from one or more in polycarboxylate, lignosulfonates, alkylphenol polyoxyethylene formaldehyde condensation products sulphate, alkyl benzene sulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol polyoxyethylene, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, the fatty acid glyceride APEO.
Described wetting agent is selected from: lauryl sodium sulfate, calcium dodecyl benzene sulfonate, draw back in powder copper rosinate X., wetting and penetrating agent F, alkylnaphthalene sulfonate, polyoxyethylene triphenylethylene phosphenylic acid salt, spaonin powder, silkworm excrement, the soapberry powder one or more.
Described disintegrant is selected from: one or more in bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, the sodium bicarbonate.
Described thickener is selected from: one or more in xanthans, CMC, hydroxyethylcellulose, methylcellulose, Magnesiumaluminumsilicate, the polyvinyl alcohol.
Described stabilizing agent is selected from: a kind of in sodium citrate, the resorcinol.
Described antifreezing agent is selected from: one or more in ethylene glycol, propane diols, the glycerine.
Described defoamer is selected from: silicone oil, silicone compound, C
10-20Saturated fat acid compounds, C
8-10In the aliphatic alcohols compound one or more.
Described filler is selected from: one or more in kaolin, diatomite, bentonite, attapulgite, white carbon, starch, the precipitated calcium carbonate.
Indoor virulence and field experiment
Indoor bioassay:
Biologicall test example 1:
(1) experimental subjects:
Bacterium of downy mildew of cucumber, separation and purification gained from the cucumber downy mildew incidence of leaf of Shouguang, Shandong cucumber booth is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
For trying medium:
PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml.
AEA medium: dusty yeast 5g/L, glycerine 20mL/L, MgSO
40.25g/L, NaNO
36g/L, KCL0.5g/L, KH
2PO
41.5g/L, agar powder 20g/L, deionized water 1L.
(2) experimental pharmacy:
The former medicine of 90%bixafen, Bayer crop science Co., Ltd, the former medicine of 96% mancozeb, Limin Chemical Co., Ltd..
Combination setting is mixed:
32%bixafen mancozeb percentage composition was respectively 5: 27,7: 25,9: 23,11: 21,13: 19, i.e. 5%+27%, 7%+25%, 9%+23%, 11%+21%, 13%+19% totally 5 combinations that are mixed.
(3) test method:
Single agent is to the toxicity test of cucumber downy mildew germ:
Bixafen and mancozeb dissolve with proper amount of acetone, are made into the mother liquor of 10000 μ g/ml, preserve in 4 ℃ of following low temperature, and be stand-by, is diluted to a series of concentration with sterile water before the test.
The cucumber downy mildew germ after pre-cultivation the on the AEA medium, is inoculated in the bacterium dish of diameter 5mm respectively on the AEA flat board that contains the variable concentrations gradient, cultivates down in 25 ℃ of dark conditions.Every processing repeats 3 times.When control strain colony diameter length is above to 6.5c m, adopt " right-angled intersection method " to measure.According to the linear regression analysis between the logarithm value of the probit value of inhibiting rate and medicine series concentration, obtain the EC that two kinds of single agent suppress mycelial growths
50Value, correlation coefficient and virulence regression equation.
On the basis of preliminary experiment, respectively single agent bixafen and mancozeb are carried out toxicity test, EC in order to last method
50Value is followed successively by 2.53mg/l and 8.46mg/l.
The mixture toxicity test:
Carry out the toxicity test of mixture according to mixed ratio with single agent toxicity test method.
EC that each that will measure according to the abundant method of Sun Yun handled
50Value is converted into the actual toxicity index (ATI) of using with; According to the proportioning of mixture, obtain theory and use toxicity index (TTI) with, calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Single agent toxicity index=(standard medicament EC
50/ reagent agent EC
50) * 100
ATI=(standard medicament EC
50/ reagent agent (using with) EC
50) * 100
The content (%) of toxicity index * B in using with of content (the %)+B of toxicity index * A of TII=A in using with
CTC＝(ATI/TTI)×100
If co-toxicity coefficient is big by 120, showing has synergistic effect; If be starkly lower than 100 (below 80), be indicated as antagonism; Between 100～120, be indicated as summation action.
(4) result and analysis:
The composite co-toxicity test of table 1bixafen and mancozeb to the cucumber downy mildew germ
| The pharmacy quality ratio | Virulence regression equation | Correlation coefficient (r) | EC 50(mg/L) |
| bixafen(A) | Y＝1.6865X+4.3203 | 0.9742 | 2.53 |
| Mancozeb (B) | Y＝1.7747X+3.3541 | 0.9533 | 8.46 |
| A: B is 5: 27 | Y＝1.6948X+3.9968 | 0.9416 | 3.91 |
| A: B is 7: 25 | Y＝1.7004X+4.1293 | 0.9507 | 3.25 |
| A: B is 9: 23 | Y＝1.8164X+4.0525 | 0.9701 | 3.32 |
| A: B is 11: 21 | Y＝1.6643X+4.1590 | 0.9664 | 3.20 |
| A: B is 13: 19 | Y＝1.7428X+4.1450 | 0.9543 | 3.09 |
The composite co-toxicity test of table 2bixafen and mancozeb to the cucumber downy mildew germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| bixafen(A) | 1∶0 | 100.00 | 100.00 | / | / |
| Mancozeb (B) | 0∶1 | 29.91 | 29.91 | / | / |
| A∶B | 5∶27 | 64.74 | 40.86 | 158.46 | Synergy |
| A∶B | 7∶25 | 77.82 | 45.24 | 172.03 | Synergy |
| A∶B | 9∶23 | 76.12 | 49.62 | 153.41 | Synergy |
| A∶B | 11∶21 | 79.04 | 54.00 | 146.37 | Synergy |
| A∶B | 13∶19 | 81.76 | 58.38 | 140.05 | Synergy |
Show according to above indoor virulence test measurement result, bixafen and mancozeb all have synergistic effect preferably by the percentage composition of above-mentioned experiment, wherein with bixafen: mancozeb is that 7: 25 o'clock co-toxicity coefficient is up to 172.03, thinks that bixafen and mancozeb percentage composition are that 7: 25 o'clock synergistic effects are best.Suggestion is carried out further field to the mixture preparation of 7: 25 left and right sides scopes of suitable proportion and is asked the test of pesticide effectiveness, asks practical application effect to estimate its field.
The composite Toxicity Determination of biologicall test example 2:bixafen and zineb to the cucumber downy mildew germ.
The former medicine of 90% zineb, Limin Chemical Co., Ltd..
The cucumber downy mildew germ, separation and purification gained from the cucumber downy mildew incidence of leaf of Shouguang, Shandong cucumber booth is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
Single agent bixafen and zineb are carried out toxicity test, EC
50Value is followed successively by 3.57mg/l and 9.76mg/l.
Combination setting is mixed:
30%bixafen zineb percentage composition was respectively 5: 25,7: 23,9: 21,11: 19,13: 17, i.e. 5%+25%, 7%+23%, 9%+21%, 11%+19%, 13%+17% totally 5 combinations that are mixed.
Test method is with biologicall test embodiment 1
Experimental result and analysis:
Table 3bixafen and the composite virulence experiment of zineb to the cucumber downy mildew germ
The composite co-toxicity experiment of table 4bixafen and zineb to the cucumber downy mildew germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| bixafen(A) | 1∶0 | 100.00 | 100.00 | / | / |
| Zineb (B) | 0∶1 | 36.58 | 36.58 | / | |
| A∶B | 5∶25 | 69.01 | 47.15 | 146.37 | Synergy |
| A∶B | 7∶23 | 78.99 | 51.38 | 153.74 | Synergy |
| A∶B | 9∶21 | 82.48 | 55.60 | 148.33 | Synergy |
| A∶B | 11∶19 | 84.38 | 59.83 | 141.03 | Synergy |
| A∶B | 13∶17 | 88.12 | 64.06 | 137.55 | Synergy |
Show according to above indoor virulence test measurement result, the used bixafen and the percentage composition of zineb all have synergistic effect in the experiment, wherein the co-toxicity coefficient when the percentage composition of bixafen and zineb is 7: 23 is 153.74 to the maximum, thinks that promptly 7: 23 o'clock synergistic effects of bixafen and zineb percentage composition are best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 7: 23 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
The composite Toxicity Determination of biologicall test example 3:bixafen and tmtd to the wheat powdery mildew germ.
The wheat powdery mildew germ, separation and purification gained from the wheat powdery mildew incidence of leaf is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
Single agent bixafen and tmtd are carried out toxicity test, EC
50Value is followed successively by 4.23mg/l and 10.17mg/l.
Combination setting is mixed:
The percentage composition of 33%bixafen ziram was respectively 4: 29,7: 26,10: 23,13: 20,16: 17, i.e. 4%+29%, 7%+26%, 10%+23%, 13%+20%, 16%+17% totally 5 combinations that are mixed.
Test method is with biologicall test embodiment 1
Result and analysis:
Table 5bixafen and the composite co-toxicity of tmtd to the wheat powdery mildew germ
Table 6bixafen and the composite co-toxicity of tmtd to the wheat powdery mildew germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| bixafen(A) | 1∶0 | 100.00 | 100.00 | / | / |
| Tmtd (B) | 0∶1 | 41.59 | 41.59 | / | / |
| A∶B | 4∶29 | 74.89 | 48.67 | 153.87 | Synergy |
| A∶B | 7∶26 | 93.51 | 53.98 | 173.23 | Synergy |
| A∶B | 10∶23 | 86.69 | 59.29 | 146.21 | Synergy |
| A∶B | 13∶20 | 92.23 | 64.60 | 142.76 | Synergy |
| A∶B | 16∶17 | 91.75 | 69.91 | 131.24 | Synergy |
Measurement result according to the test of bactericide co-toxicity, when bixafen and tmtd percentage composition are above experiment ratio, result of the test shows all synergistic effect, and the co-toxicity coefficient when ratio is 7: 26 is 173.23 to the maximum, we can think that the percentage composition of bixafen and tmtd is at 7: 26 o'clock, and synergistic effect is best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 7: 26 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
The composite Toxicity Determination of biologicall test example 4:bixafen and ziram to the apple anthracnose germ.
The apple anthracnose germ is set separation and purification gained on the anthracnose incidence of leaf from the Shandong Yantai apple, cultivates preservation in 4 ℃ of refrigerators on the PDA inclined-plane.
Single agent bixafen and ziram are carried out toxicity test, EC
50Value is followed successively by 4.56mg/l and 7.91mg/l.
Combination setting is mixed:
27%bixafen ziram percentage composition was respectively 3: 24,6: 21,9: 18,12: 15,15: 12, i.e. 3%+24%, 6%+21%, 9%+18%, 12%+15%, 15%+12% totally 5 combinations that are mixed.
Test method is with biologicall test embodiment 1
Result and analysis:
Table 7bixafen and the composite co-toxicity of ziram to the apple anthracnose germ
Table 8bixafen and the composite co-toxicity of ziram to the apple anthracnose germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| bixafen(A) | 1∶0 | 100.00 | 100.00 | / | / |
| Ziram (B) | 0∶1 | 57.65 | 57.65 | / | / |
| A∶B | 3∶24 | 91.21 | 62.35 | 146.28 | Synergy |
| A∶B | 6∶21 | 105.57 | 67.06 | 157.43 | Synergy |
| A∶B | 9∶18 | 108.91 | 71.77 | 151.76 | Synergy |
| A∶B | 12∶15 | 112.32 | 76.47 | 146.88 | Synergy |
| A∶B | 15∶12 | 111.29 | 81.18 | 137.09 | Synergy |
Show that according to above bactericide co-toxicity test determination result bixafen and ziram percentage composition are that 6: 21 o'clock co-toxicity coefficient is 157.43 to the maximum, we think that bixafen and ziram percentage composition are that 6: 21 o'clock synergistic effects are best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 6: 21 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
Field trial 1: to the preventive effect of wheat powdery mildew
(1) reagent agent
33%bixafen tmtd WP test medicine, 10%bixafen EC, 50% tmtd WP contrasts medicament.
Basic condition experimental field:
Experimental field be located at Henan, publish the medium wheat paddock of course of disease degree for experimental field, soil fertility is medium, and irrigation and drainage are convenient.Dispenser weather in March on the same day 31 is clear to cloudy, 11 ℃ of mean temperature of air, relative moisture 81%, 2～3 grades of southerlies; April 7 was fine, 18.2 ℃ of mean temperature of air, relative moisture 60%, 2～3 grades of southerlies.
(2) spraying time and method
At the 1st medicine of wheat powdery mildew their early stage (March 31) spray, (April 7) spray medicine second time sprays 2 times altogether behind the 7d.(operating pressure 0.2～0.3MPa), 750L carries out foliar spray according to the per hectare amount of liquid medicine to adopt the 3WBS-16 knapsack sprayer.
(3) pilot survey and data are handled
Investigation state of an illness radix before the medicine, test is investigated 3 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(3) drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
(4) poisoning investigation method
Whether continuous 7d range estimation medicament has poisoning to crop after the dispenser.
(5) experimental result and analysis
Table 9 is respectively organized the test effect that test medicine is handled the control wheat powdery mildew
Interpretation of result:
As shown in Table 9, the effect of 7: 26 the combination control wheat powdery mildew that is mixed of 33%bixafen tmtd is more remarkable, behind the medicine 10 days for the first time, for the second time 10 days and 20 days preventive effects 81.85% the highest, 90.89*% and 90.20% behind the medicine, 20 days preventive effects the highest are 83.68% behind the single agent 10%bixafen EC medicine second time, 20 days preventive effect is up to 83.56% behind the 50% tmtd WP medicine second time, the effect of complex preparation significantly is better than two kinds of single agent, not only significantly improved effect, and further prolonged its lasting period for wheat powdery mildew.
Field trial 2: to the preventive effect of cucumber downy mildew
(1) investigation method
Experimental field be located at Shouguang, Shandong, publish the medium cucumber field of course of disease degree for experimental field, soil fertility is medium, and irrigation and drainage are convenient.Adopt the bilinear sampling method, 5 points are got in every sub-district, look into 5 caves at every, and 2 blades in the fixing middle part of every strain fix a point to check during investigation.
Come classification according to the cucumber downy mildew grade scale, write down total strain number, diseased plant number, degree of being in a bad way, calculate diseased plant rate, disease index and preventive effect.The cucumber downy mildew grade scale is:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%～10% of whole leaf area;
5 grades: lesion area accounts for 11%～25% of whole leaf area;
7 grades: lesion area accounts for 26%～50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
Test is investigated 4 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(2) control time and number of times
Test is investigated 3 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(3) drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
(4) poisoning investigation method
Whether continuous 7d range estimation medicament has poisoning to crop after the dispenser.
(5) experimental result and analysis
Table 10 is respectively organized the test effect that test medicine is handled the control cucumber downy mildew
Interpretation of result:
Can know that by table 10 effect of 7: 23 the combination control cucumber downy mildew that is mixed of 30%bixafen zineb is very remarkable.Under the consumption of same every mu of 10ml, the effect of complex preparation significantly is better than two kinds of single agent, and its quick-acting and lasting effect are all relatively good, and is the most outstanding with lasting effect especially.Complex preparation significantly is better than effect under the low concentration consumption in control efficiency under the consumption of high concentration.20 days preventive effects reach 89.79% after 10 days best control efficiency behind the 1st, 2 medicine reaches 85.33% and 86.86%, the 2 medicine respectively.And single agent 10%bixafen EC, 10 days the highest preventive effect is 82.71% behind the 2nd medicine, 20 days preventive effects are 81.11% behind the 2nd medicine of single agent 65% zineb WP, the effect of complex preparation significantly is better than two kinds of single agent, not only significantly improved effect, and further prolonged its lasting period for the cucumber downy mildew germ.
In sum, with new type bactericide bixafen and part dithiocarbamate fungicide mancozeb, zineb, Carbatene, ziram, carry out the leaf spot of two yuan of bactericidal composition preparations that are mixed for active ingredient one of arbitrarily in the tmtd to various crop, powdery mildew, rust, downy mildew, eqpidemic disease, disease such as anthracnose and rice blast all has the better prevention effect, can effectively suppress further developing of disease, simultaneously to crop safety, suggestion is promoted the use of, in use should be used alternatingly, to delay the pesticide resistance of plant with other different mechanisms medicines.
Claims (7)
1. pesticide sterilizing composite is characterized in that: this bactericidal composition is to be C with A novel pyrazole series bactericidal agent bixafen(molecular formula
18H
12Cl
2F
3N
3O) with B part dithiocarbamates compound mancozeb, zineb, Carbatene, tmtd, ziram in one of any two yuan of preparations that are mixed that carry out for active ingredient, all the other components are usual auxiliaries in the agricultural chemicals,
A novel pyrazole series bactericidal agent bixafen.
2. bactericidal composition according to claim 1 is characterized in that: the percentage by weight of effective constituents A and B is 80: 1～1: 90 in this bactericidal composition.
3. bactericidal composition according to claim 1 is characterized in that: the percentage by weight of effective constituents A and B is 50～1: 5～70 in this bactericidal composition.
4. bactericidal composition according to claim 1 is characterized in that: to account for the percentage of gross weight in the preparation be 1%～90% for effective constituents A and B total content in this bactericidal composition.
5. bactericidal composition according to claim 4 is characterized in that: effective constituents A and the B gross weight percentage composition in preparation is preferably 5%～75%.
6. bactericidal composition according to claim 1 is characterized in that: the preparation formulation that bactericidal composition of the present invention can be mixed with is wetting powder, suspending agent, dry suspending agent, soluble liquid and water dispersible granules.
7. according to the described bactericidal composition of claim 1 to 7, it is characterized in that: bactericidal composition of the present invention is mainly used in fungoid, the bacterial disease of field crops such as control vegetables, fruit tree, corn, paddy rice, wheat.
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| CN105941440A (en) * | 2016-05-25 | 2016-09-21 | 南京华洲药业有限公司 | Sterilization composition containing Bixafen and zineb and application thereof |
| CN109645017A (en) * | 2019-01-18 | 2019-04-19 | 青岛农业大学 | Sodium diethyldithiocarbamate is in preparation for preventing and treating the application in the fungicide by the microbial plant disease of pathogenic |
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| CN1646494A (en) * | 2002-02-19 | 2005-07-27 | 拜尔农作物科学股份公司 | Disubstituted pyrazolyl carboxanilides |
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| CN1646494A (en) * | 2002-02-19 | 2005-07-27 | 拜尔农作物科学股份公司 | Disubstituted pyrazolyl carboxanilides |
| WO2005034628A1 (en) * | 2003-10-10 | 2005-04-21 | Bayer Cropscience Aktiengesellschaft | Synergistic fungicidal active substance combinations |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015079334A1 (en) * | 2013-11-26 | 2015-06-04 | Upl Limited | A method for controlling rust |
| GB2532700A (en) * | 2013-11-26 | 2016-05-25 | Upl Ltd | A method for controlling rust |
| CN105744834A (en) * | 2013-11-26 | 2016-07-06 | Upl 有限公司 | A method for controlling rust |
| GB2532700B (en) * | 2013-11-26 | 2016-08-31 | Upl Ltd | A method for controlling rust |
| US9788544B2 (en) | 2013-11-26 | 2017-10-17 | Upl Limited | Method for controlling rust |
| US10362782B2 (en) | 2013-11-26 | 2019-07-30 | Upl Limited | Method for controlling rust |
| US10499638B2 (en) | 2013-11-26 | 2019-12-10 | Upl Limited | Method for controlling rust |
| US10694744B2 (en) | 2013-11-26 | 2020-06-30 | Upl Limited | Method for controlling rust |
| US10694745B2 (en) | 2013-11-26 | 2020-06-30 | Upl Limited | Method for controlling rust |
| US10701935B2 (en) | 2013-11-26 | 2020-07-07 | Upl Limited | Method for controlling rust |
| CN105941440A (en) * | 2016-05-25 | 2016-09-21 | 南京华洲药业有限公司 | Sterilization composition containing Bixafen and zineb and application thereof |
| CN109645017A (en) * | 2019-01-18 | 2019-04-19 | 青岛农业大学 | Sodium diethyldithiocarbamate is in preparation for preventing and treating the application in the fungicide by the microbial plant disease of pathogenic |
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