CN106577680A - Bactericide and application thereof - Google Patents
Bactericide and application thereof Download PDFInfo
- Publication number
- CN106577680A CN106577680A CN201611087635.5A CN201611087635A CN106577680A CN 106577680 A CN106577680 A CN 106577680A CN 201611087635 A CN201611087635 A CN 201611087635A CN 106577680 A CN106577680 A CN 106577680A
- Authority
- CN
- China
- Prior art keywords
- antibacterial
- agent
- fluazinam
- amisulbrom
- bactericide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a bactericide composition. The effective constituents of the bactericide composition comprise amisulbrom and fluazinam with the weight ratio being 20:1 to 1:50. The bactericide composition has the benefits that firstly, the composition has obvious coordinative synergistic effect; secondly, a bacteriocidal spectrum is enlarged and the lasting period is prolonged; thirdly, the using dosage is small, the amount of residue of bactericide on crops is reduced, and environmental pollution is reduced, so that the bactericide cost is reduced; fourthly, the bactericide composition comprises effective constituents with different action mechanisms, is beneficial for overcoming and delaying the occurrence of insecticide resistance, is safe for crops, and is an important measure for preventing and treating crop diseases.
Description
Technical field
The present invention relates to a kind of composition pesticide, more particularly to a kind of antibacterial group containing amisulbrom and fluazinam
Compound and its application.
Background technology
Amisulbrom, English common name amisulbrom is the contact killing type of the triazolylsulfonamide class of Japanese Nissan exploitation
Antibacterial, has very high activity to epidemic disease and downy mildew, particularly very high to the free spore activity of pathogenic bacteria, and funguses can be suppressed to exhale
Inhale and spore germination；And with certain osmotically active and good resistance of rainwater washing against ability, correlation study finds, chemicals treatment
Have fifty percent to be impregnated with epidermis after 1d, after 8d, have eighty per cant to be impregnated with epidermis.Current research is also shown that the sprinkling indazole sulphur bacterium after bacteria infection
Amine, the blade that can make to fall ill cannot be formed and perfect spore, so as to suppress other positions to cause a disease, it is to avoid crop superinfection pathogenic bacteria；
On 2 26th, 2016, approval registration product list was intended in medicine inspecting institute of Ministry of Agriculture publicity 2016 years the 2nd batch, wherein, Japanese Nissan
The amisulbrom active compound and preparation for learning Industrial Co., Ltd will obtain registration in China head.
Fluazinam be by Japanese Shi Yuan industry companies develop new dinitroaniline protective fungicide, fungicidal spectrum
Very wide, the lasting period is 10～14 days, is mitochondrial oxidation phosphorylated uncoupling agentss, can prevent and treat the disease caused by Botrytis cinerea,
It is highly effective to interlink spore genus, Botrytis, Phytophthora, Plasmopara, Sclerotinia and Venturia.Fluazinam can suppress spore
The course of infection in pathogenic bacteria all stages such as son sprouting, mycelia breakthrough, growth and Sporulation.To epidemic disease, mildew, rotten sclerotiniose,
Melasma, scab and other pathogen diseases have good prevention effect, and in addition to bactericidal activity, fluazinam also shows
Acaricidal activity to red spider etc..
Often there is different degrees of defect in the antibacterial kind containing single-activity component on agricultural disease is prevented and treated, such as
Prevention effect is poor, and dispenser dosage is also easy to produce greatly Drug resistance, increases environmental pollution etc. application times more.For preventing and treating is agriculturally produced
The disease of resistance, has some methods such as crop structure adjustment, different pesticide rotations etc., but during practical operation, very
Hardly possible really plays obvious effect.All it is generally the complex composition for releasing the different mechanisms of action and no interactions resistance.
Different cultivars composition is compounded, and is to prevent and treat resistance to produce modal method.Prevention effect can be so improved, delays drug resistance
Property, medicament usage amount is reduced, environmental pollution is reduced, is the important means of integrated control crop pest.
Find through correlation study, downy mildew of garpe is extremely susceptible under high temperature, super-humid conditions.In recent years throughout complete
Each province of state ground, greenhouse, open country have generation, and have, and becomes one of Major Diseases.Therefore, by the mechanism of action
It is different and have the amisulbrom of certain prevention effect and fluazinam to compound downy mildew of garpe.According to test of many times,
Complex preparation fungicidal spectrum in application process is big, quick-acting is good, lasting period length and be not likely to produce Drug resistance.
The content of the invention
The present invention is intended to provide a kind of resistance risk is little, the compound disinfectant that good drug efficacy, lasting period length, environmental pollution are little,
Its effective ingredient is amisulbrom and fluazinam.
It is a further object of the present invention to provide a kind of application containing amisulbrom and the microbicide compositions of fluazinam.
In order to realize the purpose of the present invention, a kind of microbicide compositions of the present invention, its effective ingredient is amisulbrom
And fluazinam, the weight ratio of the two is 20:1～1:50.
Wherein, the weight ratio of preferred amisulbrom and fluazinam is 5:1～1:10.
Above-mentioned effective ingredient amisulbrom and fluazinam are antibacterial well known in the art, can pass through commercial channel
Buy.
Microbicide compositions of the present invention can add conven-tional adjuvants and make various differences according to this area customary preparation methods
Dosage form, such as wettable powder, suspending agent or water dispersible granules.Wherein the percentage composition of effective ingredient is 1%～80%.
On the basis of the above, carrier and auxiliary agent are also included in the microbicide compositions.
On the basis of the above, the carrier be water, one or more in filler.
On the basis of the above, described filler is Kaolin, kieselguhr, bentonite, attapulgite, white carbon, starch or light
One or more in matter Calcium Carbonate.
On the basis of the above, the auxiliary agent at least includes a kind of surfactant, and the surfactant can be dispersion
One or more in agent, wetting agent, can be nonionic or anionic, or two types compound use.
On the basis of the above, the dispersant is polycarboxylate, lignosulfonates, the contracting of alkylphenol polyoxyethylene formaldehyde
Compound sulfate, alkyl benzene calcium sulfonate, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol polyoxyethylene, aliphatic amine polyoxyethylene
One or more in ether, aliphatic acid polyethenoxy ether or glycerine fatty acid ester APEO.
On the basis of the above, the wetting agent be sodium lauryl sulphate, dodecylbenzene sodium sulfonate, Nekal BX,
One or more in T80, wetting and penetrating agent F, spaonin powder, Bombyx mori L. or spaonin powder.
On the basis of the above, according to different use occasions and demand, the auxiliary agent also includes other functions auxiliary agent, institute
Other functions auxiliary agent is stated for one or more in antifreezing agent, thickening agent, stabilizer, disintegrating agent, adhesive or defoamer.
On the basis of the above,
The antifreezing agent is one or more in ethylene glycol, Propylene Glycol, glycerol or carbamide；
The thickening agent is gelatin, sodium carboxymethyl cellulose, hydroxyethyl cellulose, sodium polyacrylate, modified starch, xanthan
One or more in glue, bentonite, silicon dioxide and aluminium-magnesium silicate；
The stabilizer be epoxy soybean oil, epoxychloropropane, BHT, ethyl acetate, one kind of triphenyl phosphate or many
Kind；
On the basis of the above, the disintegrating agent is bentonite, carbamide, ammonium sulfate, aluminum chloride, low substituted hydroxy-propyl fiber
One or more in element, Lactose, citric acid, succinic acid or sodium bicarbonate.
The adhesive be CMC, sucrose, Polyethylene Glycol, one or more in polyvinyl alcohol；
The defoamer is silicone, C10～C20 saturated fat acids compound or C8～C10 aliphatic alcohols chemical combination
One or more in thing.
Microbicide compositions of the present invention can be used to prevent and treat downy mildew of garpe.
The beneficial effects of the present invention is：1st, the inventive composition has；2nd, fungicidal spectrum expands
The big and lasting period extends.3rd, using dosage is few, reduces residual quantity of the pesticide on crop, alleviates environmental pollution, so as to drop
Low drug cost；4th, it is made up of the effective ingredient of different mechanism of action, is conducive to overcoming and delaying drug-fast generation, to makees
The features such as thing is safe, is the important means of integrated control crop pest.
Specific embodiment
The present invention is illustrated with reference to embodiments.In embodiment, percentage ratio is weight percentage, field efficacy
Treatment dosage is effective ingredient consumption.
1st, wettable powder
Preparation method：In Example formulations ratio by effective ingredient amisulbrom, fluazinam, dispersant, wetting agent, fill out
Material mixing, the mix homogeneously in mixed cylinder are remixed Jing after jet mill is crushed uniform, you can the present composition is obtained
Wettable powder product.Capital equipment is batching kettle and jet mill.
Embodiment 1：(40% amisulbrom fluazinam wettable powder)
Formula：Amisulbrom, 10%；Fluazinam, 30%；Dispersant selects lignosulfonates and naphthalenesulfonateformaldehyde formaldehyde to contract
Compound sodium salt, consumption are respectively 4% and 4%；Wetting agent selects dodecylbenzene sodium sulfonate, and 3%；Filler selects Kaolin, remaining
Amount.
2nd, suspending agent
Preparation method：By formula proportion, by effective ingredient amisulbrom and fluazinam antibacterial, surfactant and its
Its functional aid is sequentially placed in reactor, is mixed with water, Jing high speed shear, wet method sand milling, and last homogeneous filtration is
Obtain product.Capital equipment is batching kettle, colloid mill or homogeneous mixer, sand mill.
Embodiment 2：(30% amisulbrom fluazinam suspending agent)
Formula：Amisulbrom, 10%；Fluazinam, 20%；Wetting agent T80,1%；Dispersant polycarboxylate, 3%；Wood
Mahogany sulfonate, 3%；Thickening agent xanthan gum, 0.2%；Antifreezing agent Propylene Glycol, 5%；Defoamer organosilicon compound,
0.2%；Water, surplus.
3rd, water dispersible granules
Preparation method：In Example formulations ratio, effective ingredient amisulbrom is added into carrier with fluazinam antibacterial
In, and surfactant and other functional aids are added wherein, mixing adds part water Jing after comminution by gas stream, and then Jing is pinched
Conjunction, pelletize, drying, screening are obtained water dispersible granules product；Or the powder body crushed is sprayed water in boiling granulator, pelletize,
It is dried, sieves afterwards and product is obtained.Capital equipment has mixer, jet mill, kneader, Squeezinggranulator, drying room or stream
Change bed to be dried, or boiling granulator, screening machine.
Embodiment 3：(60% amisulbrom fluazinam water dispersible granules)
Formula：Amisulbrom, 10%；Fluazinam, 50%；Wetting agent dodecylbenzene sodium sulfonate, 4%；Dispersant alkyl
Naphthalenesulfonate formaldehyde condensation compound, 6%；Adhesive Polyethylene Glycol, 2%；Disintegrating agent carbamide, 4%；Filler Kaolin, surplus.
The embodiment of the present invention is the method combined using Toxicity Determination and field efficacy.First pass through indoor virulence survey
Fixed, when determining that two kinds of medicament different proportions are compounded co-toxicity coefficient (CTC), is potentiation more than 120, is short of money less than 100
Anti- effect, is summation action between 100～120.On this basis, then carry out field control effectiveness test.
Toxicity Determination：It is Pseudoperonospora cubensis mycelia for trying pathogenic bacteria；By amisulbrom and fluazinam single dose and not on year-on-year basis
5 Concentraton gradient are diluted to using acetone after example mixture.Using mycelial growth rate method, with the culture medium for adding sterilized water it is
Control, each process are repeated 3 times, and after cultivating 4-5 days, measure each process colony diameter with crossing method, press under the conditions of 25%
Below equation calculates suppression ratio, carries out data statistic analysis using SPSS.Obtain virulence regression equation, EC50And CTC.Virulence is surveyed
Determine result such as table 1：
Suppression ratio (the %)=control colony diameter of (control colony diameter-process colony diameter) × 100/
1 amisulbrom of table and inhibitory action of the fluazinam different ratio to Pseudoperonospora cubensis mycelia
| Reagent agent | Proportioning | Virulence regression equation | EC50(g/L) | CTC |
| Amisulbrom A | — | Y=1.2567x+4.4125 | 0.0594 | — |
| Fluazinam B | — | Y=1.5629x+6.6163 | 0.0867 | — |
| A:B | 5:1 | Y=1.2065x+5.6022 | 0.0498 | 123.8 |
| A:B | 3:1 | Y=1.0138x+6.2138 | 0.0338 | 142.6 |
| A:B | 2:1 | Y=1.0716x+5.4511 | 0.0199 | 169.7 |
| A:B | 1:1 | Y=0.9874x+5.5557 | 0.0101 | 203.9 |
| A:B | 1:2 | Y=0.8439x+5.4878 | 0.0089 | 245.2 |
| A:B | 1:3 | Y=0.6049x+5.4878 | 0.0075 | 289.3 |
| A:B | 1:5 | Y=0.5314x+5.0829 | 0.0050 | 313.5 |
| A:B | 1:10 | Y=0.6195x+5.5130 | 0.0097 | 221.3 |
Field control effectiveness test：Set up single medicament, embodiment medicament and using clear water as control (CK) totally 6 process.Often
Process cell 10m2, it is repeated 3 times, random district's groups arrangement selects fine day dispenser.Investigation method is with reference to Ministry of Agriculture's field control effectiveness test
Criterion, per 5 points of samplings of cell, per is investigated 5 plants, and per plant is investigated all blades, accounts for whole leaf area by lesion area on blade
Percentage rate classification.Be intended to investigate disease index before dispenser and after dispenser, and calculate actual preventive effect, difference is carried out with SPSS softwares
Significance analysis.Result of the test is as shown in table 2 below：
Disease index=∑ (the sick numbers of sheets at different levels × relative series) × 100/ investigation total number of sheets × highest series
Prevention effect (%)=(check plot disease index-treatment region disease index)/check plot disease index
Field efficacy of 2 different disposal of table to downy mildew of garpe
Note：In table, letter is diversity between each chemicals treatment, all with different lower case persons in same column, represents that difference shows
Write (P<0.05).
As known from Table 1, amisulbrom and fluazinam proportioning are 5:1 and 1:There is a certain degree of potentiation to make between 10
With.When ratio 1:When 5, its co-toxicity coefficient is maximum, with obviously potentiation.Can be seen that from 2 result of the test of table and apply
The 3rd day after medicine, compound drug to the prevention effect of downy mildew of garpe more than 80%, the 14th day preventive effect reached 90% with
On；The use of both compoundings, is significantly improved than the use preventive effect of single medicament；These illustrate that the inventive composition has preferably
Quick-acting and lasting effect.It is not difficult to find out from above table, the two compound proportion 1:Best prevention effect is reached when 5, no
Medicament usage amount is only reduced, production cost is reduced, environmental pollution is reduced.In addition, field experiment observation, said composition
The whole growth promoter of Fructus Vitis viniferae is had no adverse effects, there is tight security to people, domestic animal and environment.Summary result, indazole
Flusulfamide and fluazinam compounding has significant synergies, and delays the generation of disease drug resistance, is that to prevent and treat downy mildew of garpe non-
Normal preferably complex composition.
Claims (10)
1. a kind of antibacterial, it is characterised in that effective ingredient is amisulbrom and fluazinam.
2. bactericidal composition according to claim 1, it is characterised in that the weight ratio of the amisulbrom and fluazinam
For 20:1～1:50.
3. antibacterial according to claim 2, it is characterised in that：The weight ratio of the amisulbrom and fluazinam is 5:1
～1:10.
4. the antibacterial according to any one of claim 1-3, it is characterised in that：The weight percentage of its effective ingredient is
1%～80%.
5. antibacterial according to claim 4, it is characterised in that：The dosage form of the antibacterial is wettable powder, suspending agent
Or water dispersible granules.
6. antibacterial according to claim 5, it is characterised in that：The antibacterial also includes carrier and auxiliary agent.
7. antibacterial according to claim 6, it is characterised in that：The carrier is water or filler；The auxiliary agent includes surface
Activating agent；Described filler is in Kaolin, kieselguhr, bentonite, attapulgite, white carbon, starch or precipitated calcium carbonate
Plant or various；One or more in disintegrating agent, dispersant, wetting agent of the surfactant.
8. antibacterial according to claim 7, it is characterised in that：The antibacterial also includes function additive, and the function is helped
Agent is one or more in antifreezing agent, thickening agent, stabilizer, disintegrating agent, adhesive and defoamer.
9. the application of the antibacterial described in a kind of any one of claim 1-8, it is characterised in that for preventing and treating downy mildew.
10. the application of antibacterial according to claim 9, it is characterised in that：For preventing and treating downy mildew of garpe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611087635.5A CN106577680A (en) | 2016-12-01 | 2016-12-01 | Bactericide and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611087635.5A CN106577680A (en) | 2016-12-01 | 2016-12-01 | Bactericide and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106577680A true CN106577680A (en) | 2017-04-26 |
Family
ID=58594694
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611087635.5A Pending CN106577680A (en) | 2016-12-01 | 2016-12-01 | Bactericide and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106577680A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110100825A (en) * | 2019-05-21 | 2019-08-09 | 青岛润生农化有限公司 | Fruit insecticide composition and its application |
| CN115067340A (en) * | 2021-03-12 | 2022-09-20 | 东莞市东阳光农药研发有限公司 | Bactericidal composition, preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101052302A (en) * | 2004-10-22 | 2007-10-10 | 石原产业株式会社 | Agricultural or horticultural bactericidal composition and method of controlling plant disease |
| CN105961399A (en) * | 2015-01-12 | 2016-09-28 | 四川利尔作物科学有限公司 | Fungicidal composition and application thereof |
| CN106212471A (en) * | 2016-07-22 | 2016-12-14 | 重庆东永植保技术有限责任公司 | A kind of containing amisulbrom and the bactericidal composition of fluazinam and application |
-
2016
- 2016-12-01 CN CN201611087635.5A patent/CN106577680A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101052302A (en) * | 2004-10-22 | 2007-10-10 | 石原产业株式会社 | Agricultural or horticultural bactericidal composition and method of controlling plant disease |
| CN105961399A (en) * | 2015-01-12 | 2016-09-28 | 四川利尔作物科学有限公司 | Fungicidal composition and application thereof |
| CN106212471A (en) * | 2016-07-22 | 2016-12-14 | 重庆东永植保技术有限责任公司 | A kind of containing amisulbrom and the bactericidal composition of fluazinam and application |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110100825A (en) * | 2019-05-21 | 2019-08-09 | 青岛润生农化有限公司 | Fruit insecticide composition and its application |
| CN115067340A (en) * | 2021-03-12 | 2022-09-20 | 东莞市东阳光农药研发有限公司 | Bactericidal composition, preparation method and application thereof |
| CN115067340B (en) * | 2021-03-12 | 2023-12-08 | 东莞市东阳光农药研发有限公司 | Sterilization composition, preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101999356B (en) | Sterilization composition containing fluopicolide and metalaxyl and application thereof | |
| CN104322558A (en) | Sterilization composition and applications thereof | |
| CN106689145A (en) | Weeding composition containing pyroxasulfone and amide herbicides | |
| CN105961399A (en) | Fungicidal composition and application thereof | |
| CN105211075B (en) | Bactericidal composition and its application | |
| CN101810181A (en) | Sterilizing pesticide combination, preparation method and application thereof | |
| CN106577680A (en) | Bactericide and application thereof | |
| CN107006505A (en) | A kind of composition pesticide of copper 8-hydroxyquinolinate and pyraclostrobin | |
| CN102484994B (en) | Synergy composition of matrine and chlorantraniliprole | |
| CN104054712B (en) | Sterilization composition containing boscalid | |
| CN101779645A (en) | Azoxystrobin composite and application thereof | |
| CN106942255A (en) | Include the nematicidal composition of iprodione | |
| CN108719288B (en) | Application of farnesol as bactericidal synergist in prevention and treatment of plant gray mold | |
| CN105594705A (en) | Pesticide composition containing fluazinam and fluopyram | |
| CN104542611A (en) | Bactericidal composition containing mandipropamid | |
| CN106234367A (en) | A kind of containing fluopicolide and the bactericidal composition of methoxy acrylic | |
| CN103355309A (en) | Sterilization composition and preparation thereof | |
| CN105284843B (en) | Bactericidal composition | |
| CN102273475A (en) | High efficiency germicide composition | |
| CN106417313A (en) | Bactericidal composition containing amisulbrom and fluopicolide | |
| CN105076168A (en) | Pesticide composition containing albendazole and fluopyram | |
| CN106305751A (en) | Pesticide composition | |
| CN105104396B (en) | A kind of composition pesticide containing rosickyite azoles and amino-oligosaccharide | |
| CN104430390A (en) | Amino-oligosaccharin and ethaboxam compounded agricultural fungicide | |
| CN104068033B (en) | Fungicidal composition containing boscalid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170426 |
|
| RJ01 | Rejection of invention patent application after publication |