CN105104396B - A kind of composition pesticide containing rosickyite azoles and amino-oligosaccharide - Google Patents
A kind of composition pesticide containing rosickyite azoles and amino-oligosaccharide Download PDFInfo
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- CN105104396B CN105104396B CN201510560014.3A CN201510560014A CN105104396B CN 105104396 B CN105104396 B CN 105104396B CN 201510560014 A CN201510560014 A CN 201510560014A CN 105104396 B CN105104396 B CN 105104396B
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Abstract
The invention discloses a kind of composition pesticides containing rosickyite azoles and amino-oligosaccharide, and including the first active ingredient rosickyite azoles and the second active ingredient amino-oligosaccharide and common auxiliary element, the weight ratio of rosickyite azoles and amino-oligosaccharide is 30:1~1:10.Great number of indoor and outdoor experiments show that after being compounded, composition pesticide of the present invention has significant synergistic function, single dose exclusive use is substantially better than to the control effect of crops nematode, quick-acting is good, and the lasting period is long, while reduces Pesticide use amount, reduce residual quantity of the pesticide on plant, the pollution to environment is alleviated, has delayed the drug-fast generation of nematode, has added the Pesticide use service life.
Description
Technical field
The present invention relates to composition pesticide and its application field, it is specifically a kind of using rosickyite azoles and amino-oligosaccharide as
The composition pesticide of active ingredient, for preventing crops nematode.
Background technology
Rosickyite azoles (albendazole) is benzimidazole insecticidal bactericide.As medicine, available for ascarifuge, pinworm
Worm, tapeworm, whipworm, hookworm, excrement strongylid etc. after being metabolized as sulfoxide type or sulfone class in vivo, inhibit parasite to glucose
It absorbs, cause polypide glycogen depletion or inhibits fumaric reductase system, hinder the generation of ATP, make parasite that can not survive
And breeding.Crop pesticide has plurality of plant diseases significant preventive effect, and has certain production-increasing function, meanwhile, on plant
Nematode also has good control effect.
Amino-oligosaccharide (oligosaccharins) is D- Glucosamines with the oligosaccharide of β -1.4 glucosides key connections,
It degrades obtained after obtaining chitosan by chitin degrading or is extracted by microbial fermentation again, suppression is generated to the growth of some germs
It makes and uses, influence fungus spore germination, hypha form morphs, biochemistry changes in spore for induction, while can excite plant
Object generates the chitinase with resistant effect, dextranase, protects element and PR albumen etc., and with cell activation effect, contributes to
The recovery of aggrieved plant, promoting root growth strong sprout enhance the resistance of crop, promote growth and development of plants.Not only to fungi, bacterium, disease
Poison has extremely strong preventive effect, but also has effects that nutrition, adjusting, removing toxic substances.It can be widely used for prevention fruit tree, vegetables, underground
Rhizome, tobacco, Chinese medicine and the virus of grain and cotton crop, bacterium, fungus-caused disease.
Plant nematode is a kind of very harmful pathogenic microorganism, is had a very wide distribution, species is various, environment adapts to
Ability is strong, can colonize in root system, stem, leaf, seed, young shoot and the fruit of plant, can be by feeding activity to host plant
Mechanical damage is caused, and robs the nutrition of host plant, especially its esophageal gland secretion can cause host plant that a system occurs
Epidemy becomes, and can also infect host plant jointly with other pathogenic microorganisms, cause compound disease, cause production loss.In the whole world
In the range of, agricultural losses caused by plant nematode is annual are up to 100,000,000,000 dollars or more, seriously constrain agricultural economy
Development.
The present inventor has made intensive studies the screening of the compounded formula of rosickyite azoles and amino-oligosaccharide, find rosickyite azoles and
Amino-oligosaccharide is compounded, and has significant synergistic function, warp to Plant nematode in the range of certain compound proportion
Further research, completes the present invention.
The content of the invention
It is an object of the invention to provide a kind of composition pesticide containing rosickyite azoles and amino-oligosaccharide, applied to farming
The prevention of object nematode.
Technical solution of the present invention：A kind of composition pesticide containing rosickyite azoles and amino-oligosaccharide, active ingredient is rosickyite
The weight ratio of azoles and amino-oligosaccharide, rosickyite azoles and amino-oligosaccharide is 30:1~1:10, preferred weight ratio 10:1~1:5,
The total weight percentage composition of rosickyite azoles and amino-oligosaccharide in the composition is 0.1%~80%, remaining is to allow to make in pesticide
With with acceptable auxiliary element.
The composition pesticide can be configured to microcapsule suspending agent, suspend according to well known to a person skilled in the art method
The application forms of the suitable agricultural use such as agent, aqueous emulsion, microemulsion, water dispersible granules, wettable powder, granule and missible oil,
The production technology of various application forms belongs to existing known technology, and details are not described herein.
The auxiliary element is solvent, emulsifier, wetting agent, dispersant, stabilizer, antifreezing agent, thickener, bonding
Agent, disintegrant, antifoaming agent, bleeding agent, macromolecule cyst material, synergist, film forming agent, preservative and filler etc. are all pesticides
It commonly uses in preparation or allows the ingredient used, be not particularly limited, specific ingredient and dosage pass through simple according to recipe requirements
Experiment determines.
Composition pesticide provided by the invention, generally use spraying, the method soaked seed or spread fertilizer over the fields use, can also be according to need
It will other techniques used in agriculture may also be adopted
Compared with prior art, the advantage of the invention is that：
1st, rosickyite azoles and amino-oligosaccharide compounding have synergistic function, are significantly improved pair compared with single dose exclusive use
The control effect of nematode.
2nd, two kinds of active ingredients of rosickyite azoles and amino-oligosaccharide compounding, reduces Pesticide use amount, reduce use cost,
Alleviate the pollution to environment.
3rd, two kinds of active ingredient compoundings of rosickyite azoles and amino-oligosaccharide, the mechanism of action is different, has delayed the anti-medicine of nematode
The generation of property, the service life for adding pesticide.
Specific embodiment
To better understand the essence of the present invention, present disclosure is described further with reference to embodiment,
But limitation of the present invention cannot be considered as, it is as described below to be only used for explaining the present invention, for without departing from spirit of the invention and principle
Modification, replacement or the improvement made, belong to the scope of protection of present invention.
Embodiment one：30% rosickyite azoles amino-oligosaccharide microcapsule suspending agent.
Following substance is weight percentage：Rosickyite azoles 25%, amino-oligosaccharide 5%, tolyl isocyanate 10%, three
Toluene 10%, ethyl acetate 10%, castor oil polyoxyethylene ether 5%, calcium lignosulfonate 3%, sodium hydroxide 2%, deionization
Water complements to 100%.Above-mentioned active ingredient, macromolecule cyst material, solvent are mixed, makes to be dissolved into homogeneous oil phase, shear
Under the conditions of, by oil phase be added to containing emulsifier, dispersant, pH adjusting agent aqueous phase solution in, surplus is supplied with deionized water,
Two kinds of materials react in oil-water interfaces, form high score ascus wall, you can 30% rosickyite azoles amino-oligosaccharide micro-capsule is made
Suspending agent.
The embodiment is used to prevent cucumber root-knot nematode：30% rosickyite azoles amino-oligosaccharide microcapsule suspending agent presses 200g
A.i./ha is diluted with water root irrigation, after medicine 3d, 7d, 20d be respectively 97.48% to the preventive effect of cucumber root-knot nematode,
94.56%th, 92.29%；10% rosickyite azole suspending agent is diluted with water root irrigation by 250g a.i./ha, 3d, 7d, 20d after medicine
Preventive effect to cucumber root-knot nematode is respectively 71.36%, 65.27%, 59.48%；5% amino-oligosaccharide aqua presses 60g
A.i./ha is diluted with water root irrigation, after medicine 3d, 7d, 20d be respectively 63.42% to the preventive effect of cucumber root-knot nematode,
56.12%th, 51.54%.As it can be seen that obvious synergy after rosickyite azoles and amino-oligosaccharide binary built, quick-acting is good, holds
The effect phase is long, and the preventive effect of cucumber root-knot nematode is used alone significantly better than each single dose, meanwhile, do not found during experiment this two
First compound drug generates poisoning to cucumber.
Embodiment two：25% rosickyite azoles amino-oligosaccharide suspending agent.
Following substance is weight percentage：Rosickyite azoles 20%, amino-oligosaccharide 5%, dodecyl sodium sulfate 10%, three
Phenethyl phenol polyethenoxy ether and its phosphate 5%, polycarboxylate 5%, propylene glycol 3%, xanthans 1%, silicone oil 0.1%, go
Ionized water supplies 100%.By above-mentioned active ingredient, wetting agent, dispersant, antifreezing agent, thickener and antifoaming agent through high speed shear
It is sanded in scattered, sand mill, high dispersive, the suspension system of stabilization formed in water-medium, you can 25% rosickyite is made
Azoles amino-oligosaccharide suspending agent.
The embodiment is used to prevent watermelon root-knot：25% rosickyite azoles amino-oligosaccharide suspending agent by 200g a.i./
Ha is diluted with water root irrigation, after medicine 3d, 7d, 20d be respectively 96.48% to the preventive effect of watermelon root-knot, 93.91%,
91.43%；10% rosickyite azole suspending agent is diluted with water root irrigation by 250g a.i./ha, and 3d, 7d, 20d are to watermelon root after medicine
The preventive effect of tie lines worm is respectively 73.98%, 67.24%, 61.35%；5% amino-oligosaccharide aqua adds water by 60g a.i./ha
Root irrigation is diluted, 3d, 7d, 20d are respectively 61.36%, 56.86%, 50.51% to the preventive effect of watermelon root-knot after medicine.
As it can be seen that obvious synergy after rosickyite azoles and amino-oligosaccharide binary built, quick-acting is good, and the lasting period is long, to watermelon root
The preventive effect of tie lines worm is used alone significantly better than each single dose, meanwhile, do not find the binary built medicament to west during experiment
Melon generates poisoning.
Embodiment three：45% rosickyite azoles amino-oligosaccharide aqueous emulsion.
Following substance is weight percentage：Rosickyite azoles 35%, amino-oligosaccharide 10%, trimethylbenzene 10%, cyclohexanone
10%th, nonylphenol polyoxyethylene ether 15%, sodium lignin sulfonate 3%, xylitol 1%, glycerine 1%, deionized water is supplied
100%.Above-mentioned active ingredient, solvent, emulsifier, dispersant and stabilizer are mixed to form oil phase, antifreezing agent dissolves in water
Water phase is formed, slowly oil phase is added in water phase under cutter effect, and continues shearing about 30 minutes, 45% rosickyite is made
Azoles amino-oligosaccharide aqueous emulsion.
The embodiment is used to prevent banana root-knot nematode：45% rosickyite azoles amino-oligosaccharide aqueous emulsion presses 200g
A.i./ha is diluted with water root irrigation, after medicine 3d, 7d, 20d be respectively 98.34% to the preventive effect of banana root-knot nematode,
95.49%th, 93.75%；10% rosickyite azole suspending agent is diluted with water root irrigation by 250g a.i./ha, 3d, 7d, 20d after medicine
Preventive effect to banana root-knot nematode is respectively 70.91%, 63.57%, 56.32%；5% amino-oligosaccharide aqua presses 60g
A.i./ha is diluted with water root irrigation, after medicine 3d, 7d, 20d be respectively 61.58% to the preventive effect of banana root-knot nematode,
56.81%th, 51.37%.As it can be seen that obvious synergy after rosickyite azoles and amino-oligosaccharide binary built, quick-acting is good, holds
The effect phase is long, and the preventive effect of banana root-knot nematode is used alone significantly better than each single dose, meanwhile, do not found during experiment this two
First compound drug generates poisoning to banana.
Example IV：40% rosickyite azoles amino-oligosaccharide microemulsion.
Following substance is weight percentage：Rosickyite azoles 30%, amino-oligosaccharide 10%, trimethylbenzene 10%, cyclohexanone
10%th, Triton X-100 10%, tributyl phosphate 2%, thiophene ketone 1%, propyl isome 2%, deionized water is supplied
100%.Active ingredient with solvent is completely dissolved, emulsifier, stabilizer, bleeding agent, synergist is then added and is uniformly mixed,
Deionized water is eventually adding, after being sufficiently stirred, you can 40% rosickyite azoles amino-oligosaccharide microemulsion is made.
The embodiment is used to prevent capsicum root-knot nematode：40% rosickyite azoles amino-oligosaccharide microemulsion presses 200g
A.i./ha is diluted with water root irrigation, after medicine 3d, 7d, 20d be respectively 96.84% to the preventive effect of capsicum root-knot nematode,
93.75%th, 90.42%；10% rosickyite azole suspending agent is diluted with water root irrigation by 250g a.i./ha, 3d, 7d, 20d after medicine
Preventive effect to capsicum root-knot nematode is respectively 71.44%, 66.98%, 59.67%；5% amino-oligosaccharide aqua presses 60g
A.i./ha is diluted with water root irrigation, after medicine 3d, 7d, 20d be respectively 60.25% to the preventive effect of capsicum root-knot nematode,
53.98%th, 48.37%.As it can be seen that obvious synergy after rosickyite azoles and amino-oligosaccharide binary built, quick-acting is good, holds
The effect phase is long, and the preventive effect of capsicum root-knot nematode is used alone significantly better than each single dose, meanwhile, do not found during experiment this two
First compound drug generates poisoning to capsicum.
Embodiment five：40% rosickyite azoles amino-oligosaccharide water dispersible granules.
Following substance is weight percentage：Rosickyite azoles 35%, amino-oligosaccharide 5%, sulfosuccinic monooctyl ester sodium salt 10%,
Alkylphenol polyoxyethylene ether 10%, acrylate homopolymer sodium salt 5%, urea 2%, attapulgite supplies 100%.It will be above-mentioned effective
Ingredient, wetting agent, dispersant, disintegrant and filler are uniformly mixed, and are crushed with micro jet, through mediating, are then added in
It is granulated, dried, sieved in fluidized-bed granulation dryer, you can 40% rosickyite azoles amino-oligosaccharide water-dispersible grain is made
Agent.
The embodiment is used to prevent tomato root-knot eelworm：40% rosickyite azoles amino-oligosaccharide water dispersible granules press 200g
A.i./ha is diluted with water root irrigation, after medicine 3d, 7d, 20d be respectively 96.13% to the preventive effect of tomato root-knot eelworm,
94.87%th, 91.92%；10% rosickyite azole suspending agent is diluted with water root irrigation by 250g a.i./ha, 3d, 7d, 20d after medicine
Preventive effect to tomato root-knot eelworm is respectively 68.75%, 63.27%, 56.84%；5% amino-oligosaccharide aqua presses 60g
A.i./ha is diluted with water root irrigation, after medicine 3d, 7d, 20d be respectively 61.63% to the preventive effect of tomato root-knot eelworm,
55.24%th, 50.82%.As it can be seen that obvious synergy after rosickyite azoles and amino-oligosaccharide binary built, quick-acting is good, holds
The effect phase is long, and the preventive effect of tomato root-knot eelworm is used alone significantly better than each single dose, meanwhile, do not found during experiment this two
First compound drug generates poisoning to tomato.
Embodiment six：60% rosickyite azoles amino-oligosaccharide wettable powder.
Following substance is weight percentage：Rosickyite azoles 50%, amino-oligosaccharide 10%, fatty alcohol polyoxyethylene ether
10%th, acrylate homopolymer sodium salt 10%, clay supplies 100%.By above-mentioned by active ingredient, wetting agent, dispersant and filler
It mixes, is uniformly mixed again through air-flow crushing after mechanical crushing, you can 60% rosickyite azoles amino-oligosaccharide wettable powder is made
Agent.
The embodiment is used to prevent root knot nematode in tobacco：60% rosickyite azoles amino-oligosaccharide wettable powder presses 200g
A.i./ha is diluted with water root irrigation, after medicine 3d, 7d, 20d be respectively 98.56% to the preventive effect of root knot nematode in tobacco,
96.52%th, 92.18%；10% rosickyite azole suspending agent is diluted with water root irrigation by 250g a.i./ha, 3d, 7d, 20d after medicine
Preventive effect to root knot nematode in tobacco is respectively 72.46%, 65.53%, 58.77%；5% amino-oligosaccharide aqua presses 60g
A.i./ha is diluted with water root irrigation, after medicine 3d, 7d, 20d be respectively 63.86% to the preventive effect of root knot nematode in tobacco,
57.24%th, 52.83%.As it can be seen that obvious synergy after rosickyite azoles and amino-oligosaccharide binary built, quick-acting is good, holds
The effect phase is long, and the preventive effect of root knot nematode in tobacco is used alone significantly better than each single dose, meanwhile, do not found during experiment this two
First compound drug generates poisoning to tobacco.
Embodiment seven：5% rosickyite azoles amino-oligosaccharide granule.
Following substance is weight percentage：Rosickyite azoles 4%, amino-oligosaccharide 1%, lauryl sodium sulfate 10%, alkane
Base phenol polyethenoxy ether 15%, polyvinyl alcohol 20%, kaolin complements to 100%.By above-mentioned active ingredient, wetting agent, disperse
Agent, binding agent and filler are mediated through mixing and water adding, are pinched out and shaped using pinch-out granulator, afterwards by particle fluid bed
Dry, screening, you can 5% rosickyite azoles amino-oligosaccharide granule is made.
The embodiment is used to prevent peanut root-knot nematode：5% rosickyite azoles amino-oligosaccharide granule by 200g a.i./
Ha spreads fertilizer over the fields processing, and 3d, 7d, 20d are respectively 94.72%, 91.35%, 87.26% to the preventive effect of peanut root-knot nematode after medicine；
10% rosickyite azole suspending agent is diluted with water root irrigation by 250g a.i./ha, and 3d, 7d, 20d are to peanut root-knot nematode after medicine
Preventive effect is respectively 69.27%, 63.53%, 57.24%；5% amino-oligosaccharide aqua is diluted with water pouring root by 60g a.i./ha
It handles, 3d, 7d, 20d are respectively 60.51%, 53.29%, 47.63% to the preventive effect of peanut root-knot nematode after medicine.As it can be seen that rosickyite
Obvious synergy after azoles and amino-oligosaccharide binary built, quick-acting is good, and the lasting period is long, and peanut root-knot nematode is prevented
Effect is used alone significantly better than each single dose, meanwhile, do not find that the binary built medicament generates poisoning to peanut during experiment.
Embodiment eight：40% rosickyite azoles amino-oligosaccharide missible oil.
Following substance is weight percentage：Rosickyite azoles 33%, amino-oligosaccharide 7%, trimethylbenzene 10%, cyclohexanone
10%th, styrenated phenol polyoxyethylene ether 20%, double to kill 3%, solvent naphtha complements to 100%.Above-mentioned active ingredient is added in molten
It is fully dissolved after agent, then addition emulsifier, synergist become the oily liquids of transparent and homogeneous after stirring evenly, you can are made
40% rosickyite azoles amino-oligosaccharide missible oil.
The embodiment is used to prevent ginger root-knot nematode：40% rosickyite azoles amino-oligosaccharide missible oil presses 200g a.i./ha
Be diluted with water root irrigation, after medicine 3d, 7d, 20d be respectively 95.15% to the preventive effect of ginger root-knot nematode, 93.67%,
90.59%；10% rosickyite azole suspending agent is diluted with water root irrigation by 250g a.i./ha, and 3d, 7d, 20d are to race after medicine
The preventive effect of tie lines worm is respectively 71.68%, 67.24%, 62.04%；5% amino-oligosaccharide aqua adds water by 60g a.i./ha
Root irrigation is diluted, 3d, 7d, 20d are respectively 62.54%, 56.31%, 51.79% to the preventive effect of ginger root-knot nematode after medicine.
As it can be seen that obvious synergy after rosickyite azoles and amino-oligosaccharide binary built, quick-acting is good, and the lasting period is long, to race
The preventive effect of tie lines worm is used alone significantly better than each single dose, meanwhile, do not find the binary built medicament to life during experiment
Ginger generates poisoning.
Embodiment nine：Indoor joint toxicity measuring.
In order to further appreciate that the synergy type compounded using rosickyite azoles and amino-oligosaccharide as active ingredient, the present invention
People has carried out substantial amounts of Toxicity Determination experiment.
1st, rosickyite azoles and amino-oligosaccharide are to the indoor joint toxicity measuring of cucumber root-knot nematode.
Test method：The cucumber root for generating a large amount of root knots is taken first, is gently rinsed well with tap water, then with dissection
The pin gently milky egg capsule in picking old complaint, is put into the small culture dish of diameter 6cm, adds in a small amount of sterile water, in 25 DEG C of perseverances
Hatch 3~4d in incubator, collect 2 instar larvaes, and add in sterile water and be configured to the outstanding of a certain concentration (500/ml or so)
Supernatant liquid is spare.
5 concentration gradient processing of setting, using sterile water as blank control.96 porocyte culture plates are taken, adds in and prepares per hole
Various concentration 150 microlitres of medicament, add isometric nematode suspension, moisturizing culture under the conditions of 25 DEG C, after treatment
The amount of survival and The dead quantity of 48h microscopy nematodes, nematode is in stiff motionless for dead worm, and nematode is in bending wriggling state to live
Worm.Calculate the death rate and corrected mortality.
Statistical analysis is carried out with DPS statistical analysis softwares, calculates the LC of each medicament50, and mixture is calculated according to the abundant methods of Sun Yun
Co-toxicity coefficient (CTC values).
Survey toxicity index (ATI)=(standard agent LC50/ reagent agent LC50)×100。
Percentage composition+B the medicament toxicity index of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
The percentage composition of B in agent.
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI)] × 100.
The criteria for classifying is acted on according to NY/T11547.7-2006 insecticide combinations：Co-toxicity coefficient (CTC) >=120 is shown as
Synergistic effect；Co-toxicity coefficient (CTC)≤80 shows as antagonism；80 ＜ co-toxicity coefficients (CTC) ＜ 120, which show as being added, to be made
With.
Table 1, rosickyite azoles and amino-oligosaccharide are to the indoor joint toxicity measuring result of cucumber root-knot nematode
| Medicament title and proportioning (weight ratio) | LC50(mg/L) | ATI | TTI | CTC |
| Rosickyite azoles | 9.81 | 100.00 | - | - |
| Amino-oligosaccharide | 17.23 | 56.94 | - | - |
| Rosickyite azoles：Amino-oligosaccharide (30:1) | 6.59 | 148.86 | 98.61 | 150.96 |
| Rosickyite azoles：Amino-oligosaccharide (20:1) | 5.67 | 173.02 | 97.95 | 176.64 |
| Rosickyite azoles：Amino-oligosaccharide (10:1) | 4.95 | 198.18 | 96.09 | 206.24 |
| Rosickyite azoles：Amino-oligosaccharide (5:1) | 4.28 | 229.21 | 92.82 | 246.94 |
| Rosickyite azoles：Amino-oligosaccharide (1:1) | 5.64 | 173.94 | 78.47 | 221.66 |
| Rosickyite azoles：Amino-oligosaccharide (1:5) | 7.43 | 132.03 | 64.12 | 205.91 |
| Rosickyite azoles：Amino-oligosaccharide (1:10) | 9.26 | 105.94 | 60.85 | 174.10 |
As it can be seen from table 1 rosickyite azoles and amino-oligosaccharide are 30:1~1:When being compounded in the range of 10 (by weight), altogether
Malicious coefficient (CTC) is all higher than 120, and synergistic function is shown to cucumber root-knot nematode；Rosickyite azoles and amino-oligosaccharide exist
10:1~1:In the range of 5 (by weight), synergistic function is more notable, and co-toxicity coefficient (CTC) is more than 200；Rosickyite
Azoles and amino-oligosaccharide are with 5:1 (by weight) compounding, synergistic function is the most notable, and co-toxicity coefficient (CTC) is 246.94.It can
See, rosickyite azoles and amino-oligosaccharide compounding have reasonability and feasibility.
2nd, rosickyite azoles and amino-oligosaccharide are to the indoor joint toxicity measuring of root knot nematode in tobacco.
Test method：Field tobacco root knot sample is gathered, tissue mashing crosses 200,300,500 mesh nematode standard screens successively,
With the worm's ovum on 500 mesh sieve of aseptic water washing, ovum suspended emulsion is configured to, hatches 3-4d in 25 DEG C of insulating boxs, collects two ages children
Worm simultaneously adds in sterile water to be configured to a certain concentration (about 500/ml) suspended emulsion spare.
5 concentration gradient processing of setting, using sterile water as blank control.96 porocyte culture plates are taken, adds in and prepares per hole
Various concentration 150 microlitres of medicament, add isometric nematode suspension, moisturizing culture under the conditions of 25 DEG C, after treatment
The amount of survival and The dead quantity of 48h microscopy nematodes, nematode is in stiff motionless for dead worm, and nematode is in bending wriggling state to live
Worm.Calculate the death rate and corrected mortality.
Statistical analysis is carried out with DPS statistical analysis softwares, calculates the LC of each medicament50, and mixture is calculated according to the abundant methods of Sun Yun
Co-toxicity coefficient (CTC values).
Survey toxicity index (ATI)=(standard agent LC50/ reagent agent LC50)×100。
Percentage composition+B the medicament toxicity index of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
The percentage composition of B in agent.
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI)] × 100.
The criteria for classifying is acted on according to NY/T11547.7-2006 insecticide combinations：Co-toxicity coefficient (CTC) >=120 is shown as
Synergistic effect；Co-toxicity coefficient (CTC)≤80 shows as antagonism；80 ＜ co-toxicity coefficients (CTC) ＜ 120, which show as being added, to be made
With.
Table 2, rosickyite azoles and amino-oligosaccharide are to the indoor joint toxicity measuring result of root knot nematode in tobacco
| Medicament title and proportioning (weight ratio) | LC50(mg/L) | ATI | TTI | CTC |
| Rosickyite azoles | 11.06 | 100.00 | - | - |
| Amino-oligosaccharide | 20.67 | 53.51 | - | - |
| Rosickyite azoles：Amino-oligosaccharide (30:1) | 6.35 | 174.17 | 98.50 | 176.82 |
| Rosickyite azoles：Amino-oligosaccharide (20:1) | 5.81 | 190.36 | 97.79 | 194.66 |
| Rosickyite azoles：Amino-oligosaccharide (10:1) | 5.03 | 219.88 | 95.77 | 229.59 |
| Rosickyite azoles：Amino-oligosaccharide (5:1) | 4.67 | 236.83 | 92.25 | 256.73 |
| Rosickyite azoles：Amino-oligosaccharide (1:1) | 6.24 | 177.24 | 76.76 | 230.90 |
| Rosickyite azoles：Amino-oligosaccharide (1:5) | 8.83 | 125.25 | 61.26 | 204.46 |
| Rosickyite azoles：Amino-oligosaccharide (1:10) | 10.47 | 105.64 | 57.74 | 182.96 |
From table 2 it can be seen that rosickyite azoles and amino-oligosaccharide are 30:1~1:When being compounded in the range of 10 (by weight), altogether
Malicious coefficient (CTC) is all higher than 120, and synergistic function is shown to root knot nematode in tobacco；Rosickyite azoles and amino-oligosaccharide exist
10:1~1:In the range of 5 (by weight), synergistic function is more notable, and co-toxicity coefficient (CTC) is more than 200；Rosickyite
Azoles and amino-oligosaccharide are with 5:1 (by weight) compounding, synergistic function is the most notable, and co-toxicity coefficient (CTC) is 256.73.It can
See, rosickyite azoles and amino-oligosaccharide compounding have reasonability and feasibility.
In conclusion the nematicidal composition that the present invention is compounded using two kinds of active components rosickyite azoles and amino-oligosaccharide,
Obvious synergy, compared with existing unitary agent, quick-acting is good, and the lasting period is long, prevents nematode raising with obvious effects,
To crop safety, it is worth promoting and applying in agricultural production.
Claims (4)
1. a kind of composition pesticide containing rosickyite azoles and amino-oligosaccharide, it is characterised in that：Active ingredient is rosickyite azoles and ammonia
The weight ratio of base oligosaccharide, rosickyite azoles and amino-oligosaccharide is 30:1~1:10, rosickyite azoles and amino-oligosaccharide are in the composition
Total weight percentage composition be 0.1%~80%.
2. a kind of composition pesticide containing rosickyite azoles and amino-oligosaccharide according to claim 1, it is characterised in that：Institute
The weight ratio for stating rosickyite azoles and amino-oligosaccharide is 10:1~1:5.
3. a kind of composition pesticide containing rosickyite azoles and amino-oligosaccharide according to claim 1, it is characterised in that：Institute
The dosage form for stating composition pesticide be microcapsule suspending agent, suspending agent, aqueous emulsion, microemulsion, water dispersible granules, wettable powder,
One kind in granula and missible oil.
4. a kind of composition pesticide containing rosickyite azoles and amino-oligosaccharide according to claim 1 is in prevention crops line
Application in worm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510560014.3A CN105104396B (en) | 2015-09-04 | 2015-09-04 | A kind of composition pesticide containing rosickyite azoles and amino-oligosaccharide |
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| CN201510560014.3A CN105104396B (en) | 2015-09-04 | 2015-09-04 | A kind of composition pesticide containing rosickyite azoles and amino-oligosaccharide |
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| CN105104396B true CN105104396B (en) | 2018-05-18 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283226A (en) * | 2011-09-15 | 2011-12-21 | 湖南大方农化有限公司 | Agricultural composition containing albendazole for preventing and treating plant root-knot nematodes |
| CN102388879A (en) * | 2011-10-28 | 2012-03-28 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide containing composition used for killing nematodes and application and method thereof |
| CN102870780A (en) * | 2012-09-25 | 2013-01-16 | 河北科技师范学院 | Preparation capable of inducing cucumber to resist meloidogyne and preparation method for preparation |
| CN104430427A (en) * | 2014-12-30 | 2015-03-25 | 贵州道元生物技术有限公司 | Root-knot nematode preventing pesticide |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283226A (en) * | 2011-09-15 | 2011-12-21 | 湖南大方农化有限公司 | Agricultural composition containing albendazole for preventing and treating plant root-knot nematodes |
| CN102388879A (en) * | 2011-10-28 | 2012-03-28 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide containing composition used for killing nematodes and application and method thereof |
| CN102870780A (en) * | 2012-09-25 | 2013-01-16 | 河北科技师范学院 | Preparation capable of inducing cucumber to resist meloidogyne and preparation method for preparation |
| CN104430427A (en) * | 2014-12-30 | 2015-03-25 | 贵州道元生物技术有限公司 | Root-knot nematode preventing pesticide |
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| CN105104396A (en) | 2015-12-02 |
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