CN102388879A - Chitosan oligosaccharide containing composition used for killing nematodes and application and method thereof - Google Patents
Chitosan oligosaccharide containing composition used for killing nematodes and application and method thereof Download PDFInfo
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- CN102388879A CN102388879A CN2011103326663A CN201110332666A CN102388879A CN 102388879 A CN102388879 A CN 102388879A CN 2011103326663 A CN2011103326663 A CN 2011103326663A CN 201110332666 A CN201110332666 A CN 201110332666A CN 102388879 A CN102388879 A CN 102388879A
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- chitosan oligosaccharide
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Abstract
The invention relates to a chitosan oligosaccharide containing composition used for killing nematodes and application and a method thereof. The invention specifically provides a pesticide composition used for killing nematodes. The pesticide composition contains chitosan oligosaccharide, wherein the chitosan oligosaccharide accounts for 0.045-0.011% of the pesticide composition by weight. The invention also provides application of the pesticide composition containing chitosan oligosaccharide in controlling nematodes and the method for controlling nematodes by applying the composition containing chitosan oligosaccharide. The composition, application and the method have the following beneficial effects: the important problem of killing nematodes is solved; and in particular, the pesticide composition containing chitosan oligosaccharide not only kills nematodes but also can not cause chemical injury on the crops and does not have pesticide residues after treating the crops suffering from nematodiasis, thus realizing environment-friendly control of nematodes.
Description
Technical field
The invention belongs to agrochemical field, particularly relate to the control composition of nematode and prevent and treat method, relate to a kind of chitosan oligosaccharide composition and use thereof and method that is used to kill nematode particularly.
Background technology
Plant nematode each plant species that causes harm all has parasitism from low liver moss, type of fainting, algae, the mushroom that waits to high naked son and angiosperm.With regard to the parasitic site, all can be parasitic at root system of plant, young shoot, stem, leaf, flower, seed and fruit.In actual production, nematode and some fungi, bacterium, virus interact, and cause compound infecting.Therefore, to some degree, nematode has meaning dual even the multiplicity cause of disease.Plant nematode is caused about 1,000 hundred million dollars of the loss that world agriculture produces every year.The loss that China causes because of nematode endangers is up to 23,300,000,000 yuan.
The method of preventing and treating of root-knot nematode is a lot, and is common except that plant quarantine, also has use, physical control and the biological control etc. of crop rotation diseases prevention, nematicide breed of variety, nematicide medicament.The exploitation of nematocide is less; Available scope is less; That existing most of kind is faced with is expensive, high poison, high residue, preventive effect are undesirable, or even produce poisoning, cause environmental pollution, problems such as ecological disruption, soil fertility decline, food security.From the angle of the Scientific Outlook on Development, long-term use and agricultural sustainable development that chemistry kills the line medicament are contradiction, make the chemical control work of root-knot nematode face very big predicament.That biological control has is environmentally friendly, realize advantage such as agricultural sustainable development, and therefore, the biological control that meets modern agriculture strategy of sustainable development theory becomes the first-selected measure that root-knot nematode is administered.
Inventor of the present invention is through long-term field research, and acquisition can effectively prevent and treat the chitosan oligosaccharide agricultural chemicals of nematode, finds that chitosan oligosaccharide is effective chemical substance of control nematode.And through further investigation, obtained the control nematode chitosan oligosaccharide concentration, application process and use period.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of composition pesticide that is used to kill nematode, comprise chitosan oligosaccharide in this composition pesticide, the percentage by weight that chitosan oligosaccharide accounts for this composition pesticide is 0.045%-0.011%.Preferably, to account for the percentage by weight of this composition pesticide be 0.005%-0.009% to chitosan oligosaccharide.
Above-mentioned this composition pesticide can prepare liquid preparation or solid pharmaceutical preparation.Liquid preparation can be any liquid preparation of agriculture usefulness, and solid pharmaceutical preparation also is any solid pharmaceutical preparation of agriculture usefulness.Preferably, liquid preparation is an aqua, microemulsion, water dispersible granules or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or granule.
The present invention also provides the above-mentioned composition pesticide that comprises chitosan oligosaccharide to be used to prevent and treat the purposes of nematode; The percentage by weight that it is characterized in that comprising chitosan oligosaccharide in the composition pesticide of chitosan oligosaccharide is 0.045%-0.011%; Preferably, to account for the percentage by weight of composition be 0.005%-0.009% to chitosan oligosaccharide.
The present invention also provides the method for using the composition for preventing and controlling nematode that comprises chitosan oligosaccharide; It is characterized in that using composition at the initial stage that nematodosis is sent out, every then at a distance from 5-10 days time, preferred whenever at a distance from time of 7 days; Use once more and the identical or different composition of application concentration first 0-3 time; Preferably, use composition 2 times, the percentage by weight of chitosan oligosaccharide is 0.045%-0.011% in the wherein said composition; Preferably, to account for the percentage by weight of composition be 0.005%-0.009% to chitosan oligosaccharide.
In the said method, said composition can prepare liquid preparation or solid pharmaceutical preparation.Liquid preparation can be any liquid preparation of agriculture usefulness, and solid pharmaceutical preparation also is any solid pharmaceutical preparation of agriculture usefulness.Preferably, liquid preparation is an aqua, microemulsion, water dispersible granules or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or granule.
In the said method, any method effectively commonly used when the method for using is meant the applications of pesticide includes but not limited to powder injection process, spray-on process, fumigating system, soil treatment method or smog method etc.
Wherein powder injection process is meant that the wind-force that utilizes duster to produce is sprinkling upon the agricultural chemicals of pulvis the surface of crop or controlling object equably; Spray-on process is meant with a certain amount of agricultural chemicals and an amount of water and is made into soup, with the spraying apparatus medicine liquid spray is become droplet, is evenly distributed in crop or controlling object surface; Fumigating system is meant and adopts fumigant or volatile medicament, makes its volatilization become the effectively gas of prevention and elimination of disease and pests; The soil treatment method is meant and takes to spray, dusts, spreads methods such as pesticide-clay mixture, imposes on agricultural chemicals equably in ground or the certain soil layer; The smog method is meant utilizes special-purpose facility to be dispersed into smoke condition to the oily agricultural chemicals, to reach the method for Pesticidal and sterilizing weeding.
In the said method; The initial stage that nematodosis is sent out is meant that nematodosis sends out the stage that has just begun, ordinary circumstance, and this stage can be judged through range estimation to those skilled in the art; When the nematode damage to crops is just arranged, the stage that particularly has the nematode insect population to occur.Particularly, can judge also that be the initial stage that nematodosis is sent out the period below 3% that the plant of nematode insect population appearance just accounts for total plant quantity through the mode of detection by quantitative.
Among the present invention, chitosan oligosaccharide is that Glucosamine passes through 1, and the degree of polymerization that the 4-glycosidic bond is formed by connecting is the mixture of the high molecular polymer of 2-30, and mean molecule quantity is 1000-3000 dalton.Chitosan oligosaccharide can be through any means preparation, and for example following method: deacetylation is the mixture of the chitosan oligomer of 2-30 at the shitosan more than 70% through the degree of polymerization that methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation method prepare.Perhaps, be the mixture of the chitosan oligomer of 2-30 through from fungal cell wall, extracting the degree of polymerization that obtains chitin and prepare through methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods.Perhaps, through passing through 1 by Glucosamine, the degree of polymerization that the chemosynthesis of 4-glycosidic bond is polymerized is the mixture of polymers of 2-30.Chitosan oligosaccharide is the mixture of the high molecular polymer of various polymerization thing at present, that is to say in the chitosan oligosaccharide to be not the oligosaccharides form of the single degree of polymerization, but the chitosan oligosaccharide monose of every kind of degree of polymerization possibly exist all.So no matter prepare through which kind of method; The commercially available prod that perhaps prepares through above-mentioned preparation method, as long as it is that Glucosamine passes through 1, the degree of polymerization that the 4-glycosidic bond is formed by connecting is that 2-30, mean molecule quantity are that the mixture of the daltonian high molecular polymer of 1000-3000 is exactly a chitosan oligosaccharide of the present invention; It can contain acetyl group; But the sugar unit of bonding acetyl group is no more than 40% amount, preferably is no more than 30% amount, especially preferably is no more than 20% amount.Commercially available chitosan oligosaccharide all is the chitosan oligosaccharide product that above-mentioned chitosan oligosaccharide term is set forth basically at present.
Beneficial effect of the present invention:
The present invention has found to be used to kill the composition pesticide that comprises chitosan oligosaccharide of nematode, and this composition pesticide can be killed nematode, has particularly found the special effect of chitosan oligosaccharide when killing nematode of certain concentration; The major issue of nematode is killed in solution; Especially use the composition pesticide that comprises chitosan oligosaccharide after handling plant, not only kill nematode, and can be to not doing the deposits yields poisoning; Do not have residue of pesticide, realized the control of the nematode of environment protection-type.The contribution that the present invention is special also is to use good chance and the concentration that chitosan oligosaccharide is prevented and treated nematode through having discovered, has improved the effect of chitosan oligosaccharide control nematode greatly.
Embodiment
In order to understand the present invention, further specify the present invention with embodiment below, but do not limit the present invention.
Embodiment 1:
5wt% chitosan oligosaccharide solution with water is diluted to application corresponding concentration respectively; Concentration is calculated according to weight percent concentration; In outcome record, calculate with ppm (1,000,000/) concentration, what 50ppm represented in the outcome record below is that weight percent concentration is 0.005%.Clear water is handled as contrast.Solution after the dilution is in the cucumber of nematode their early stage according to the demand treatment by stages, and be 7 days blanking time that sprays processing.Each processing is observed and is detected through 3 experimental field (100 square metres), adds up the PD situation of the nematode of every experimental field respectively.Be that nematode nibbles and bites the plant quantity that cucumber has an insect population and account for 3 ± 0.25% of the total plant number of cucumber the opportunity of using chitosan oligosaccharide solution-treated nematode.The nematoda control effect is through the ratio situation of change statistics of inspection insect population quantity.
When adding up, the insect population number of control efficiency=(the insect population number of the insect population number-processing ensuing crop of blank)/blank * 100% carries out assembly average according to every experimental field; For example plant is counted the X strain in 100 square metres the experimental field; Calculate the average population number of X strain; Comprise and handling and blank that the calculating that is not always the case is calculated average control efficiency with this.
Concrete disposition and effect are following:
The table 1 nematode insect pest initial stage is used the control efficiency of chitosan oligosaccharide solution to nematode
No matter show through above-mentioned experiment, be that single treatment nematodosis is sent out the cucumber at initial stage or handled the cucumber that nematodosis is sent out the initial stage continuously, all nematode produced good control efficiency, can control the PD of nematode, kills nematode until reaching.Obtain when chitosan oligosaccharide concentration is 45-150ppm, can both producing effectively preventing to nematode, just weight percent concentration is that the chitosan oligosaccharide solution control nematode of 0.0045-0.015% is effective, and control efficiency is relatively good when concentration is 50-90ppm.
Claims (4)
1. a composition pesticide that is used to kill nematode comprises chitosan oligosaccharide in this composition pesticide, and the percentage by weight that chitosan oligosaccharide accounts for this composition pesticide is 0.045%-0.011%.Preferably, to account for the percentage by weight of this composition pesticide be 0.005%-0.009% to chitosan oligosaccharide.
2. the composition pesticide that comprises chitosan oligosaccharide is used to prevent and treat the purposes of nematode; The percentage by weight that it is characterized in that comprising chitosan oligosaccharide in the composition pesticide of chitosan oligosaccharide is 0.045%-0.011%; Preferably, to account for the percentage by weight of composition be 0.005%-0.009% to chitosan oligosaccharide.
3. use the method for the composition for preventing and controlling nematode that comprises chitosan oligosaccharide, it is characterized in that using composition, every separated 5-10 days then time at the initial stage that nematodosis is sent out; Preferred every at a distance from 7 days time, use once more and the identical or different composition of application concentration 0-3 time first, preferably; Use composition 2 times; The percentage by weight of chitosan oligosaccharide is 0.045%-0.011% in the wherein said composition, and preferred, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.005%-0.009%.
4. method according to claim 3, wherein initial stage of sending out of nematodosis is meant the period below 3% that plant that the nematode insect population occurs accounts for total plant quantity.
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| CN2011103326663A CN102388879A (en) | 2011-10-28 | 2011-10-28 | Chitosan oligosaccharide containing composition used for killing nematodes and application and method thereof |
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| CN2011103326663A CN102388879A (en) | 2011-10-28 | 2011-10-28 | Chitosan oligosaccharide containing composition used for killing nematodes and application and method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102870780A (en) * | 2012-09-25 | 2013-01-16 | 河北科技师范学院 | Preparation capable of inducing cucumber to resist meloidogyne and preparation method for preparation |
| CN103636637A (en) * | 2013-12-04 | 2014-03-19 | 郭红甫 | Pesticide composition for preventing and treating crop root nematodosis by foliage spray |
| CN104488872A (en) * | 2014-10-30 | 2015-04-08 | 东莞市瑞德丰生物科技有限公司 | Nematicide composition |
| CN105104396A (en) * | 2015-09-04 | 2015-12-02 | 广东中迅农科股份有限公司 | Pesticide composition containing albendazole and amino-oligosaccharin |
| CN111727971A (en) * | 2020-06-11 | 2020-10-02 | 中科禾一（大连）农业科技有限公司 | Nematode inhibitor containing chitin oligosaccharide and application thereof |
| CN115997768A (en) * | 2023-01-10 | 2023-04-25 | 安徽省林业科学研究院 | Plant immunity induced resistance agent for preventing pine wood nematode disease and preparation method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102870780A (en) * | 2012-09-25 | 2013-01-16 | 河北科技师范学院 | Preparation capable of inducing cucumber to resist meloidogyne and preparation method for preparation |
| CN103636637A (en) * | 2013-12-04 | 2014-03-19 | 郭红甫 | Pesticide composition for preventing and treating crop root nematodosis by foliage spray |
| CN103636637B (en) * | 2013-12-04 | 2015-10-28 | 郭红甫 | A kind of composition pesticide of foliage-spray control crop root nematodosis |
| CN104488872A (en) * | 2014-10-30 | 2015-04-08 | 东莞市瑞德丰生物科技有限公司 | Nematicide composition |
| CN105104396A (en) * | 2015-09-04 | 2015-12-02 | 广东中迅农科股份有限公司 | Pesticide composition containing albendazole and amino-oligosaccharin |
| CN105104396B (en) * | 2015-09-04 | 2018-05-18 | 广东中迅农科股份有限公司 | A kind of composition pesticide containing rosickyite azoles and amino-oligosaccharide |
| CN111727971A (en) * | 2020-06-11 | 2020-10-02 | 中科禾一（大连）农业科技有限公司 | Nematode inhibitor containing chitin oligosaccharide and application thereof |
| CN115997768A (en) * | 2023-01-10 | 2023-04-25 | 安徽省林业科学研究院 | Plant immunity induced resistance agent for preventing pine wood nematode disease and preparation method thereof |
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Address after: 571924 the development of Hainan County of Chengmai province old town village distribution Applicant after: Hainan Zhengye Zhongnong High Technology Co., Ltd. Address before: Four, building 25, glorious cinema tower, 570206 Nansha Road, Longhua District, Hainan, Haikou Applicant before: Hainan Zhengye Zhongnong High Technology Co., Ltd. |
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Application publication date: 20120328 |