CN102365949B - Chitosan oligosaccharide composition for resisting hot pepper virus disease as well application and method thereof - Google Patents
Chitosan oligosaccharide composition for resisting hot pepper virus disease as well application and method thereof Download PDFInfo
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- CN102365949B CN102365949B CN 201110333131 CN201110333131A CN102365949B CN 102365949 B CN102365949 B CN 102365949B CN 201110333131 CN201110333131 CN 201110333131 CN 201110333131 A CN201110333131 A CN 201110333131A CN 102365949 B CN102365949 B CN 102365949B
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a chitosan oligosaccharide composition for resisting a hot pepper virus disease as well application and a method thereof, and in particular relates to a pesticide composition for resisting a hot pepper virus disease. The pesticide composition comprises chitosan oligosaccharide, wherein the weight percentage of chitosan oligosaccharide is 0.0035%-0.025% of weight of the pesticide composition. The invention also provides application of the pesticide composition comprising chitosan oligosaccharide for preventing and treating the hot pepper virus disease. The pesticide composition is characterized in that the chitosan oligosaccharide is 0.0035%-0.025% by weight of the pesticide composition comprising chitosan oligosaccharide. By using the composition provided by the invention, the important problem of resisting the hot pepper virus disease is solved, especially, after the pesticide composition comprising chitosan oligosaccharide is used for treating the seeds and plants of hot pepper, the hot pepper virus disease is resisted, crops are not damaged, and no pesticide is remained.
Description
Technical field
The invention belongs to agrochemical field, particularly relate to the control composition of pepper virus disease and prevent and treat method, relate to particularly a kind of chitosan oligosaccharide composition and use thereof and method for anti-pepper virus disease.
Background technology
Pepper virus disease has another name called mosaic disease, toxinosis, crazy disease etc., all is on the rise throughout the country in recent years.It is one of main disease of capsicum, and spring, autumns two, Ji Jun had generation, and it is the heaviest to delay the capsicum morbidity with autumn capsicum, autumn.Since the harm of virus disease, general pimento type underproduction 5%-10%, the long angle green pepper type underproduction about 10%.
Pepper virus disease is mainly caused by cucumber mosaic virus and tobacco mosaic virus.Tobacco mosaic virus can be in the residual branch of the diseased plant of drying long term survival, also can be malicious by seed belt, infect via the juice contact transmission.Usually high temperature and drought, during the aphid serious harm cucumber mosaic virus harm also serious, for many years continuous cropping, low-lying land, fertilizer deficiency or use the fertilizer that does not become thoroughly decomposed all can increase the weight of the harm of tobacco mosaic virus.
Mainly be with matrine, clean, the viral A of Ji lice, brassin etc. for the prevention of pepper virus disease, with the liquor potassic permanganate seed soaking, with the seed soaking of 10% trisodium phosphate solution, or spray Rogor, execute the Trane, ZhiBingLing(SIC) etc. sprays and prevents and treats.
There is not at present fully agricultural chemicals and the scientific and effective method of preventing and treating that can either environmental protection can effectively prevent and treat again pepper virus disease
The present inventor is by long-term field research, and acquisition can effectively prevent and treat the chitosan oligosaccharide agricultural chemicals of pepper virus disease, finds that chitosan oligosaccharide is effective chemical substance of control pepper virus disease.And by further investigation, obtained the control pepper virus disease chitosan oligosaccharide concentration, application process and use period.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of composition pesticide for anti-pepper virus disease, comprise chitosan oligosaccharide in this composition pesticide, the percentage by weight that chitosan oligosaccharide accounts for this composition pesticide is 0.0035%-0.025%.Preferably, to account for the percentage by weight of this composition pesticide be 0.009%-0.022% or 0.0035%-0.008% to chitosan oligosaccharide.
In the above-mentioned composition, pepper virus disease is the disease that any virus that can produce on the capsicum causes, and the chitosan oligosaccharide composition shows to the control of pepper virus disease and on the whole control be separately every kind of virus disease of control that its preventive effect is highly significant also.Pepper virus disease includes but not limited to cucumber mosaic virus and tobacco mosaic virus etc.
Above-mentioned this composition pesticide can prepare liquid preparation or solid pharmaceutical preparation.Liquid preparation can be any liquid preparation of agriculture usefulness, and solid pharmaceutical preparation also is any solid pharmaceutical preparation of agriculture usefulness.Preferably, liquid preparation is aqua, microemulsion, water dispersible granules or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or granule.
The present invention also provides the above-mentioned composition pesticide that comprises chitosan oligosaccharide to be used for the purposes of control pepper virus disease, the percentage by weight that it is characterized in that comprising chitosan oligosaccharide in the composition pesticide of chitosan oligosaccharide is 0.0035%-0.025%, preferably, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.0035%-0.008%, perhaps preferred, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.009%-0.022%.
The present invention also provides the method for using the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that the stages set of applications compound that capsicum grows is processed capsicum, comprise pepper seed is processed, perhaps use composition at chilli seedling, perhaps use composition flowering stage in capsicum, perhaps after blooming, capsicum uses composition, perhaps to pepper seed, seedling stage, before blooming, use successively composition after blooming, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.025% in the wherein said composition, preferably, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.0035%-0.008%, perhaps preferred, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.009%-0.022%.
In the said method, described composition can prepare liquid preparation or solid pharmaceutical preparation.Liquid preparation can be any liquid preparation of agriculture usefulness, and solid pharmaceutical preparation also is any solid pharmaceutical preparation of agriculture usefulness.Preferably, liquid preparation is aqua, microemulsion, water dispersible granules or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or granule.
The present invention also provides the method for using the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that pepper seed is processed, to account for the percentage of composition weight be 0.01%-0.03% to chitosan oligosaccharide in the wherein said composition, preferably, to account for the percentage of composition weight be 0.009%-0.022% to chitosan oligosaccharide.The method of wherein pepper seed being processed can be the method for treating seeds of this area routine, includes but not limited to seed dressing, dipping, seed soaking or vexed kind.Wherein seed dressing refers to medicinal powder or liquid and seed are mixed; Dipping be medicine liquid spray on seed, and constantly stir and make seed all wetting, cover the coverings such as mat, pile vexed rear sowing; Seed soaking refers to that a seed or seedling are immersed in the certain density liquid, makes seed or seedling absorb medicament through certain hour; Vexed kind refers to liquid and seed are mixed the method that the vexed certain hour of rear heap is sowed again.
The present invention also provides the method for using the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that in the seedling stage of capsicum capsicum being used composition, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.0035%-0.008% in the composition that comprises chitosan oligosaccharide of at every turn using.
The present invention also provides the method for using the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that before the blooming of capsicum, capsicum being used composition, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.0035%-0.008% in the composition that comprises chitosan oligosaccharide of at every turn using.
The present invention also provides the method for using the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that after capsicum blooms, capsicum being used composition, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.0035%-0.008% in the composition that comprises chitosan oligosaccharide of at every turn using.
The present invention also provides the method for using the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that with described composition pepper seed being processed, to account for the percentage of composition weight be 0.009%-0.022% to chitosan oligosaccharide in the wherein said composition, then in the seedling stage of capsicum capsicum is used composition, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.008% in the composition that comprises chitosan oligosaccharide of at every turn using, then applying said compositions before the blooming of capsicum, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.008% in the composition that comprises chitosan oligosaccharide of at every turn using, then applying said compositions after capsicum blooms, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.0035%-0.008% in the composition that comprises chitosan oligosaccharide of at every turn using.
In the said method, the method for wherein pepper seed being processed can be the method for treating seeds of this area routine, includes but not limited to seed dressing, dipping, seed soaking or vexed kind.Wherein seed dressing refers to medicinal powder or liquid and seed are mixed; Dipping be medicine liquid spray on seed, and constantly stir and make seed all wetting, cover the coverings such as mat, pile vexed rear sowing; Seed soaking refers to that a seed or seedling are immersed in the certain density liquid, makes seed or seedling absorb medicament through certain hour; Vexed kind refers to liquid and seed are mixed the method that the vexed certain hour of rear heap is sowed again.
In the said method, any method of effectively commonly using the when method of using refers to the applications of pesticide includes but not limited to powder injection process, spray-on process, fumigating system, soil treatment method or smog method etc.
Wherein powder injection process refers to that the wind-force that utilizes duster to produce is sprinkling upon the agricultural chemicals of pulvis the surface of crop or controlling object equably; Spray-on process refers to be made into liquid with a certain amount of agricultural chemicals and an amount of water, with the spraying apparatus medicine liquid spray is become droplet, is evenly distributed in crop or controlling object surface; Fumigating system refers to adopt fumigant or volatile medicament, makes its volatilization become the effectively gas of prevention and elimination of disease and pests; The soil treatment method refers to take spray, dusts, spreads the methods such as pesticide-clay mixture, and agricultural chemicals is imposed in ground or the certain soil layer equably; The smog method refers to utilize special-purpose facility that the oily pesticide dispersion is become smoke condition, to reach the method for Pesticidal and sterilizing weeding.
In the said method, with composition seed is processed, referred to composition is mixed with seed, composition is in the situation of liquid, and seed is soaked in the liquid, when composition is solid pharmaceutical preparation, seed is mixed with solid pharmaceutical preparation.
Among the present invention, chitosan oligosaccharide is that the degree of polymerization that Glucosamine is formed by connecting by Isosorbide-5-Nitrae-glycosidic bond is the mixture of the high molecular polymer of 2-30, and mean molecule quantity is 1000-3000 dalton.Chitosan oligosaccharide can prepare by any means, and such as following method: deacetylation is the mixture of the chitosan oligomer of 2-30 at the shitosan more than 70% by the degree of polymerization that the methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation method prepare.Perhaps, be the mixture of the chitosan oligomer of 2-30 by from fungal cell wall or insect body wall, extracting the degree of polymerization that obtains chitin and prepare by methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods.Perhaps, by being the mixture of the polymer of 2-30 by Glucosamine by the degree of polymerization that Isosorbide-5-Nitrae-the glycosidic bond chemosynthesis is polymerized.Chitosan oligosaccharide is the mixture of the high molecular polymer of different polymer at present, that is to say in the chitosan oligosaccharide to be not the oligosaccharides form of the single degree of polymerization, but the chitosan oligosaccharide monose of every kind of degree of polymerization may exist all.So no matter by which kind of method prepare; the commercially available prod that perhaps prepares by above-mentioned preparation method; as long as it is that Glucosamine passes through 1; the degree of polymerization that the 4-glycosidic bond is formed by connecting is that 2-30, mean molecule quantity are that the mixture of the daltonian high molecular polymer of 1000-3000 is exactly chitosan oligosaccharide of the present invention; it can contain acetyl group; but the sugar unit of bonding acetyl group is no more than 40% amount, preferably is no more than 30% amount, especially preferably is no more than 20% amount.Commercially available chitosan oligosaccharide all is the chitosan oligosaccharide product that above-mentioned chitosan oligosaccharide term is set forth basically at present.
Above-mentioned composition of the present invention and method can reduce or eliminate the symptom that all virus diseases can cause, virus disease symptoms such as flower leaf type, fern blade profile, necrotic type, leaf roll type in performance in the control pepper virus disease.
The symptom of flower leaf type is the bright arteries and veins of hypopathia strain blade, slight floral leaf.Grave illness strain blade presents the significantly dark green floral leaf alternate with pale green, and the blade face is not open and flat, often with bubble spot, mottled.Later stage plant top internode shortens, blade diminishes and be clump bunch shape, and the result is few and little.The symptom of fern blade profile is diseased plant leaf-shrinkage deformity, and spire narrows down, and severe one forms linear leaf, as the fern leaf.Poor growth, plant is short and small.The symptom of necrotic type is diseased plant portion of tissue chlorosis, and browning is downright bad, and main manifestations be downright bad mottled, broken spot, encircle spot, streak, rubber leaf line spot, growing point are withered etc.The symptom of leaf roll type is yellow between diseased plant blade arteries and veins, upsweeps to be spoonful shape or tubular, plant atrophy, poor growth.Catch an illness early can not the result.
More than several symptoms can occur separately, also can be accompanied.Cause to occur dark, alternate piebald, strumae, fruit deformity and fallen leaves, fallen flowers, the shedding of light green color on the fruit, so that whole strain death etc.
Beneficial effect of the present invention:
The present invention has found to be used for the composition pesticide that comprises chitosan oligosaccharide of anti-pepper virus disease, this composition pesticide can resist pepper virus disease, particularly found the special effect of chitosan oligosaccharide when anti-pepper virus disease of certain concentration, solve the major issue of anti-pepper virus disease, especially use the composition pesticide that comprises chitosan oligosaccharide behind the seed and plant of processing capsicum, anti-pepper virus disease not only, and can be to not doing the deposits yields poisoning, do not have residue of pesticide, realized the control of the pepper virus disease of environment protection-type.The special contribution of the present invention also is to have found to use good chance and the concentration that chitosan oligosaccharide is prevented and treated pepper virus disease by research, has greatly improved the effect of chitosan oligosaccharide control pepper virus disease.
Embodiment
In order to understand the present invention, the below further specifies the present invention with embodiment, but does not limit the present invention.
Embodiment 1:
5wt% chitosan oligosaccharide solution with water is diluted to respectively corresponding application concentration, concentration is calculated according to weight percent concentration, calculate with ppm (1,000,000/) concentration in outcome record, what 50ppm represented in the outcome record below is that weight percent concentration is 0.005%.Clear water is processed in contrast.According to demand treatment by stages pepper seed and pepper plant, seed treatment is that seed soaks with the solution after the dilution, and soak time is 24 hours.The processing mode in other stage is that the chitosan oligosaccharide aqua sprays processing, and be 15 days blanking time that sprays processing.Each processing is observed and is detected by 3 experimental field (667 square metres), adds up when the sample of processing seed grows up to 4 leaves after crop germinates, and the statistics of processing for the stages in vegetative period was added up after dispenser in 7 days.Add up respectively the percentage of the capsicum diseased plant generation of every experimental field, just its plant is defined as the virus disease plant as long as produced the feature of virus disease.Concrete disposition and effect are as follows:
Table 1 seed immersion treatment is to the control efficiency of pepper virus disease
Table 2 seedling spraying is to the control efficiency of pepper virus disease
Before blooming, table 3 sprays the control efficiency to pepper virus disease
After blooming, table 4 sprays the control efficiency to pepper virus disease
Table 5 is omnidistance processes capsicum to the control efficiency of pepper virus disease
No matter show by above-mentioned experiment, be to process, bloom pre-treatment or bloom post processing in treatment by stages pepper seed, seedling stage, and chitosan oligosaccharide solution has all shown good anti-pepper virus disease effect.All use chitosan oligosaccharide solution each period in capsicum, show especially the preventive effect of good anti-pepper virus disease.
Claims (9)
1. the composition pesticide that comprises chitosan oligosaccharide is used for the purposes of control pepper virus disease, and the percentage by weight that it is characterized in that comprising chitosan oligosaccharide in the composition pesticide of chitosan oligosaccharide is 0.0035%-0.025%.
2. purposes according to claim 1, wherein to account for the percentage by weight of composition be 0.0035%-0.008% to chitosan oligosaccharide, perhaps, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.009%-0.022%.
3. use the method for the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that the stages set of applications compound that capsicum grows is processed capsicum, comprise pepper seed is processed, perhaps use composition at chilli seedling, perhaps use composition flowering stage in capsicum, perhaps after blooming, capsicum uses composition, perhaps to pepper seed, seedling stage, bloom front, use successively composition after blooming, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.025% in the wherein said composition.
4. method according to claim 3, wherein to account for the percentage by weight of composition be 0.0035%-0.008% to chitosan oligosaccharide, perhaps, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.009%-0.022%.
5. use the method for the composition for preventing and controlling pepper virus disease comprise chitosan oligosaccharide, it is characterized in that pepper seed is processed, to account for the percentage of composition weight be 0.009%-0.022% to chitosan oligosaccharide in the wherein said composition.
6. use the method for the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that in the seedling stage of capsicum capsicum being used composition, use first and used 0-2 time again in rear every interval 15-20 days, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.0035%-0.008% in the composition that comprises chitosan oligosaccharide of at every turn using.
7. use the method for the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that before the blooming of capsicum, capsicum being used composition, use first and used 0-2 time again in rear every interval 15-20 days, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.0035%-0.008% in the composition that comprises chitosan oligosaccharide of at every turn using.
8. use the method for the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that after capsicum blooms, capsicum being used composition, use first and used 0-2 time again in rear every interval 15-20 days, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.0035%-0.008% in the composition that comprises chitosan oligosaccharide of at every turn using.
9. use the method for the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that with described composition pepper seed being processed, to account for the percentage of composition weight be 0.009%-0.022% to chitosan oligosaccharide in the wherein said composition, then in the seedling stage of capsicum capsicum is used composition, use first and used 0-2 time again in rear every interval 15-20 days, the concentration of at every turn using is identical or different with the concentration of using first, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.008% in the composition that comprises chitosan oligosaccharide of at every turn using, then applying said compositions before the blooming of capsicum, use first and used 0-2 time again in rear every interval 15-20 days, the concentration of at every turn using is identical or different with the concentration of using first, the percentage by weight of chitosan oligosaccharide is 0.0035%-0.008% in the composition that comprises chitosan oligosaccharide of at every turn using, then applying said compositions after capsicum blooms, use first and used 0-2 time again in rear every interval 15-20 days, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.0035%-0.008% in the composition that comprises chitosan oligosaccharide of at every turn using.
Priority Applications (1)
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| CN1520723A (en) * | 2003-01-30 | 2004-08-18 | 中国科学院上海植物生理研究所 | Chitosan oligosaccharide containging pesticide composition and its application |
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| 中国科学院大连化学物理研究所.寡糖植物疫苗研究与推广.《科技成果》.2011,成果简介. |
| 寡糖植物疫苗研究与推广;中国科学院大连化学物理研究所;《科技成果》;20110101;成果简介 * |
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