CN102388878A - Chitosan oligosaccharide composition used for resisting tobacco common mosaic as well as use and method thereof - Google Patents
Chitosan oligosaccharide composition used for resisting tobacco common mosaic as well as use and method thereof Download PDFInfo
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- CN102388878A CN102388878A CN2011103325783A CN201110332578A CN102388878A CN 102388878 A CN102388878 A CN 102388878A CN 2011103325783 A CN2011103325783 A CN 2011103325783A CN 201110332578 A CN201110332578 A CN 201110332578A CN 102388878 A CN102388878 A CN 102388878A
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Abstract
The invention relates to a chitosan oligosaccharide composition used for resisting tobacco common mosaic as well as the use and method thereof, particularly to a pesticide composition used for resisting tobacco common mosaic. The pesticide composition comprises chitosan oligosaccharide, and the weight percentage of chitosan oligosaccharide in the pesticide composition is 0.004% to 0.01% or 0.022% to 0.035%. The invention further provides the use and application method of the pesticide composition containing chitosan oligosaccharide and used for resisting tobacco common mosaic. The composition provided by the utility model solves the important problem of resisting tobacco common mosaic; and particularly after the seeds and the plants of tobacco are processed by the pesticide composition containing chitosan oligosaccharide, not only can tobacco common mosaic be resisted, but also no pesticide damage is caused to the crop, and no pesticide remains.
Description
Technical field
The invention belongs to agrochemical field, particularly relate to the control composition of tobacco mosaic and prevent and treat method, relate to a kind of chitosan oligosaccharide composition and use thereof and method that is used for resisting tobacco mosaic disease particularly.
Background technology
Tobacco mosaic is one of important disease on tobacco produces, and generally takes place in each tobacco planting district of China.The output that has a strong impact on tobacco leaf and the quality of this disease are to perplex the difficult problem that tobacco produces for a long time.
Du Yu light etc. has been studied the influence of chitosan oligosaccharide to tobacco defensive ferment activity and isodynamic enzyme zymogram.With yellow seedling elm tobacco is the examination material, frictional inoculation method inoculation tobacco mosaic virus, colorimetric method for determining several kinds of defensive ferment activity, this excites the possibility of a series of disease-resistant biochemical reactions of generation to the research chitosan oligosaccharide mutually as system to tobacco 2 tobacco mosaic viruss.Preliminary experiment results shows: chitosan oligosaccharide is handled and can be caused tobacco leaf peroxidase (PO), catalase (CAT), superoxide dismutase (SOD), polyphenol oxidase (PPO), PAL (PAL) and β 21,3 dextranase activities that raising is in various degree arranged.Learnt that by PO isodynamic enzyme enzyme spectrum analysis the virus inoculation processor can see that 7 enzyme bands and bands of a spectrum look dense behind the spray medicine, enzymic activity is strong.Tentatively think thus antiviral mechanism that chitosan oligosaccharide induces with improve PO enzyme active and that increase the isodynamic enzyme zymogram and have the pass; Analysis learns, as if the antiviral mechanism that SOD, CAT and PP03 kind enzyme are induced with chitosan oligosaccharide is relevant not quite or do not have a correlation; Monofactorial spray medicine with connect many several kinds of defensive ferments active raising, especially PAL and β 21,3 dextranase activities of can causing of poison and improve obviously, live and change and as if the rising amplitude have nothing to do with two monofactorial weighting effects but connect malicious person's enzyme behind the spray medicine.This shows that chitosan oligosaccharide has good activity to the plurality of enzymes of tobacco, can utilize its inducing anti-disease mechanism to realize control, reduce economic loss tobacco virus.
Guo Chengjin etc. have studied the inducing anti-disease property of chitosan oligosaccharide to tobacco mosaic virus.After chitosan oligosaccharide (10 μ g/mL-200 μ g/mL) spraying was handled, tobacco plant was expressed strong system induction disease resistance to tobacco mosaic virus.Inoculation leaf and newborn leaf system system symptom occur later, morbidity average retardation 3 days, and sx, the severity rank on average lowers 3 grades.After chitosan oligosaccharide (10 μ g/mL-200 μ g/mL) spraying was handled, tobacco mesophyll cell chlorophyll content was than not spraying the high 24.0%-87.6% of medicine inoculation plant.In addition, handle the health tobacco plant with chitosan oligosaccharide, chlorophyll content also has rising.Chitosan oligosaccharide is induced all in various degree enhancings of defensive ferment activity in the tobacco plant of back.Some enzymic activity can be excited in advance, and a plurality of peak values appear in some enzymic activity, and also some enzyme remains greater activity.This possibly have confidential relation with inducing anti-disease mechanism.More than research for the rational Application of chitosan oligosaccharide preparation with deeply carry out disease-resistant Mechanism Study basic foundation be provided.
But, do not provide the good method of a cover of preventing and treating tobacco mosaic in the control of tobacco mosaic.
The present invention has found out one and has been enclosed within composition, purposes and the method that big Tanaka prevents and treats tobacco mosaic on the basis of long-term field experiment.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of composition pesticide that is used for resisting tobacco mosaic disease, comprise chitosan oligosaccharide in this composition pesticide, the percentage by weight that chitosan oligosaccharide accounts for this composition pesticide is 0.022%-0.035% or 0.004%-0.01%.
In the above-mentioned composition, tobacco mosaic is the disease that any virus that can produce on the tobacco causes, and the chitosan oligosaccharide composition shows control on the whole to the control of tobacco mosaic, is every kind of virus disease of control separately, and its preventive effect is highly significant also.Tobacco mosaic includes but not limited to tobacco mosaic viruses (TMV), cucumber mosaic virus (CMV) or marmor upsilon (PVY) etc.
Above-mentioned this composition pesticide can prepare liquid preparation or solid pharmaceutical preparation.Liquid preparation can be any liquid preparation of agriculture usefulness, and solid pharmaceutical preparation also is any solid pharmaceutical preparation of agriculture usefulness.Preferably, liquid preparation is an aqua, microemulsion, water dispersible granules or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or granule.
The present invention also provides the above-mentioned composition pesticide that comprises chitosan oligosaccharide to be used to prevent and treat the purposes of tobacco mosaic; The percentage by weight that it is characterized in that comprising chitosan oligosaccharide in the composition pesticide of chitosan oligosaccharide is 0.004%-0.01%, and perhaps to account for the percentage by weight of composition be 0.022%-0.035% to chitosan oligosaccharide.
The present invention also provides the method for using the composition for preventing and controlling tobacco mosaic that comprises chitosan oligosaccharide; It is characterized in that each stage set of applications compound that tobacco grows is handled tobacco, comprise tobacco seed is handled, perhaps use composition at tobacco in seedling stage; Perhaps use composition flowering stage tobacco; Perhaps after tobacco blooms, use composition, perhaps to tobacco seed, seedling stage, bloom preceding, use composition successively after blooming, the percentage by weight of chitosan oligosaccharide is 0.004%-0.01% in the wherein said composition; Perhaps preferred, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.022%-0.035%.
In the said method, said composition can prepare liquid preparation or solid pharmaceutical preparation.Liquid preparation can be any liquid preparation of agriculture usefulness, and solid pharmaceutical preparation also is any solid pharmaceutical preparation of agriculture usefulness.Preferably, liquid preparation is an aqua, microemulsion, water dispersible granules or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or granule.
The present invention also provides the method for using the composition for preventing and controlling tobacco mosaic that comprises chitosan oligosaccharide, it is characterized in that tobacco seed is handled, and to account for the percentage of composition weight be 0.022%-0.035% to chitosan oligosaccharide in the wherein said composition.The method of wherein tobacco seed being handled can be the conventional method for treating seeds in this area, includes but not limited to seed dressing, dipping, seed soaking or vexed kind.Wherein seed dressing is meant medicinal powder or soup and seed is mixed; Dipping be medicine liquid spray on seed, and constantly stir and make seed all wetting, cover coverings such as mat, pile the sowing of vexed back; Seed soaking is meant that a seed or seedling are immersed in the certain density soup, makes seed or seedling absorb medicament through certain hour; Vexed kind is meant mixes the method that the back vexed certain hour of heap is sowed again with soup and seed.
The present invention also provides the method for using the composition for preventing and controlling tobacco mosaic that comprises chitosan oligosaccharide; It is characterized in that tobacco being used composition in the seedling stage of tobacco; Use the back first used 1 time in every interval 15-20 days again; Application times is 0-2 time once more, and the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.004%-0.01% in the composition of at every turn using that comprises chitosan oligosaccharide.
The present invention also provides the method for using the composition for preventing and controlling tobacco mosaic that comprises chitosan oligosaccharide; It is characterized in that before the blooming of tobacco, tobacco being used composition; Use the back first used 1 time in every interval 15-20 days again; Application times is 0-2 time once more, and the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.004%-0.01% in the composition of at every turn using that comprises chitosan oligosaccharide.
The present invention also provides the method for using the composition for preventing and controlling tobacco mosaic that comprises chitosan oligosaccharide; It is characterized in that after tobacco blooms, tobacco being used composition; Use the back first used 1 time in every interval 15-20 days again; Application times is 0-2 time once more, and the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.004%-0.01% in the composition of at every turn using that comprises chitosan oligosaccharide.
The present invention also provides the method for using the composition for preventing and controlling tobacco mosaic that comprises chitosan oligosaccharide; It is characterized in that with said composition tobacco seed being handled, to account for the percentage of composition weight be 0.022%-0.035% to chitosan oligosaccharide in the wherein said composition, in the seedling stage of tobacco tobacco used composition then; Use the back first used 1 time in every interval 15-20 days again; Application times is 0-2 time once more, and the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.004%-0.01% in the composition of at every turn using that comprises chitosan oligosaccharide; Then at the preceding applying said compositions of blooming of tobacco; Use the back first and used 1 time in every interval 15-20 days again, application times is 0-2 time once more, and the concentration of at every turn using is identical or different with the concentration of using first; The percentage by weight of chitosan oligosaccharide is 0.004%-0.01% in the composition of at every turn using that comprises chitosan oligosaccharide; Applying said compositions after tobacco blooms is used the back first and was used 1 time in every interval 15-20 days again then, and application times is 0-2 time once more; The concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.004%-0.01% in the composition of at every turn using that comprises chitosan oligosaccharide.
In the said method, the method for wherein tobacco seed being handled can be the conventional method for treating seeds in this area, includes but not limited to seed dressing, dipping, seed soaking or vexed kind.Wherein seed dressing is meant medicinal powder or soup and seed is mixed; Dipping be medicine liquid spray on seed, and constantly stir and make seed all wetting, cover coverings such as mat, pile the sowing of vexed back; Seed soaking is meant that a seed or seedling are immersed in the certain density soup, makes seed or seedling absorb medicament through certain hour; Vexed kind is meant mixes the method that the back vexed certain hour of heap is sowed again with soup and seed.
In the said method, any method effectively commonly used when the method for using is meant the applications of pesticide includes but not limited to powder injection process, spray-on process, fumigating system, soil treatment method or smog method etc.
Wherein powder injection process is meant that the wind-force that utilizes duster to produce is sprinkling upon the agricultural chemicals of pulvis the surface of crop or controlling object equably; Spray-on process is meant with a certain amount of agricultural chemicals and an amount of water and is made into soup, with the spraying apparatus medicine liquid spray is become droplet, is evenly distributed in crop or controlling object surface; Fumigating system is meant and adopts fumigant or volatile medicament, makes its volatilization become the effectively gas of prevention and elimination of disease and pests; The soil treatment method is meant and takes to spray, dusts, spreads methods such as pesticide-clay mixture, imposes on agricultural chemicals equably in ground or the certain soil layer; The smog method is meant utilizes special-purpose facility to be dispersed into smoke condition to the oily agricultural chemicals, to reach the method for Pesticidal and sterilizing weeding.
In the said method, with composition seed is handled, be meant composition is mixed with seed, composition is under the situation of liquid, and seed is soaked in the liquid, when composition is solid pharmaceutical preparation, seed is mixed with solid pharmaceutical preparation.
Among the present invention, chitosan oligosaccharide is that Glucosamine passes through 1, and the degree of polymerization that the 4-glycosidic bond is formed by connecting is the mixture of the high molecular polymer of 2-30, and mean molecule quantity is 1000-3000 dalton.Chitosan oligosaccharide can be through any means preparation, and for example following method: deacetylation is the mixture of the chitosan oligomer of 2-30 at the shitosan more than 70% through the degree of polymerization that methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation method prepare.Perhaps, be the mixture of the chitosan oligomer of 2-30 through from fungal cell wall or insect body wall, extracting the degree of polymerization that obtains chitin and prepare through methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods.Perhaps, through passing through 1 by Glucosamine, the degree of polymerization that the chemosynthesis of 4-glycosidic bond is polymerized is the mixture of polymers of 2-30.Chitosan oligosaccharide is the mixture of the high molecular polymer of various polymerization thing at present, that is to say in the chitosan oligosaccharide to be not the oligosaccharides form of the single degree of polymerization, but the chitosan oligosaccharide monose of every kind of degree of polymerization possibly exist all.So no matter prepare through which kind of method; The commercially available prod that perhaps prepares through above-mentioned preparation method, as long as it is that Glucosamine passes through 1, the degree of polymerization that the 4-glycosidic bond is formed by connecting is that 2-30, mean molecule quantity are that the mixture of the daltonian high molecular polymer of 1000-3000 is exactly a chitosan oligosaccharide of the present invention; It can contain acetyl group; But the sugar unit of bonding acetyl group is no more than 40% amount, preferably is no more than 30% amount, especially preferably is no more than 20% amount.Commercially available chitosan oligosaccharide all is the chitosan oligosaccharide product that above-mentioned chitosan oligosaccharide term is set forth basically at present.
Above-mentioned composition of the present invention and method can reduce or eliminate the symptom that all virus diseases can cause in performance when preventing and treating tobacco mosaic.
Beneficial effect of the present invention:
The present invention has found to be used for the composition pesticide that comprises chitosan oligosaccharide of resisting tobacco mosaic disease; This composition pesticide can resisting tobacco mosaic disease; Particularly find the special effect of chitosan oligosaccharide when resisting tobacco mosaic disease of certain concentration, solved the major issue of resisting tobacco mosaic disease, especially used the composition pesticide that comprises chitosan oligosaccharide behind the seed and plant of handling tobacco; Resisting tobacco mosaic disease not only; And can not have residue of pesticide to doing the deposits yields poisoning, realized the control of the tobacco mosaic of environment protection-type.Contribution that the present invention is special also is to use good chance and the concentration that chitosan oligosaccharide is prevented and treated tobacco mosaic through having discovered, improved the effect that chitosan oligosaccharide is prevented and treated tobacco mosaic greatly.
Embodiment
In order to understand the present invention, further specify the present invention with embodiment below, but do not limit the present invention.
Embodiment 1:
5wt% chitosan oligosaccharide solution with water is diluted to application corresponding concentration respectively; Concentration is calculated according to weight percent concentration; In outcome record, calculate with ppm (1,000,000/) concentration, what 50ppm represented in the outcome record below is that weight percent concentration is 0.005%.Clear water is handled as contrast.According to demand treatment by stages tobacco seed and tobacco plant, seed treatment is that seed soaks with the solution after the dilution, and soak time is 24 hours.The processing mode in other stage is for the chitosan oligosaccharide aqua sprays processing, and be 15 days blanking time that sprays processing.Each processing is observed and is detected through 3 experimental field (667 square metres), adds up when the sample of handling seed grows up to 4 leaves after crop germinates, and adds up in 7 days after dispenser for the statistics of each phase process in vegetative period.Add up the percentage of the tobacco diseased plant generation of every experimental field respectively, just its plant is defined as the virus disease plant as long as produced the characteristic of virus disease.Concrete disposition and effect are following:
Table 1 seed immersion treatment is to the control efficiency of tobacco mosaic
Table 2 seedling spraying is to the control efficiency of tobacco mosaic
Before blooming, table 3 sprays control efficiency to tobacco mosaic
After blooming, table 4 sprays control efficiency to tobacco mosaic
The omnidistance control efficiency of handling tobacco to tobacco mosaic of table 5
No matter show through above-mentioned experiment, be to handle, bloom pre-treatment or bloom post processing in treatment by stages tobacco seed, seedling stage, and chitosan oligosaccharide solution has all shown good resisting tobacco mosaic disease effect.All use chitosan oligosaccharide solution each period tobacco, show the preventive effect of good resisting tobacco mosaic disease especially.And find in the present invention seed treatment optium concentration and the vegetation period optimization process concentration, just be best with 220-350ppm, and handle that optium concentration is that 50-90ppm is best in the growth stage in seed treatment concentration.
Claims (8)
1. a composition pesticide that is used for resisting tobacco mosaic disease comprises chitosan oligosaccharide in this composition pesticide, and the percentage by weight that chitosan oligosaccharide accounts for this composition pesticide is 0.022%-0.035% or 0.004%-0.01%.
2. the composition pesticide that comprises chitosan oligosaccharide is used to prevent and treat the purposes of tobacco mosaic; The percentage by weight that it is characterized in that comprising chitosan oligosaccharide in the composition pesticide of chitosan oligosaccharide is 0.004%-0.01%, and perhaps to account for the percentage by weight of composition be 0.022%-0.035% to chitosan oligosaccharide.
3. use the method for the composition for preventing and controlling tobacco mosaic that comprises chitosan oligosaccharide; It is characterized in that each stage set of applications compound that tobacco grows is handled tobacco; Comprise tobacco seed is handled, perhaps use composition, perhaps use composition flowering stage tobacco at tobacco in seedling stage; Perhaps, tobacco uses composition after blooming; Perhaps to tobacco seed, seedling stage, bloom preceding, use composition successively after blooming, the percentage by weight of chitosan oligosaccharide is 0.004%-0.01% in the wherein said composition, perhaps to account for the percentage by weight of composition be 0.022%-0.035% to chitosan oligosaccharide.
4. use the method for the composition for preventing and controlling tobacco mosaic comprise chitosan oligosaccharide, it is characterized in that tobacco seed is handled, to account for the percentage of composition weight be 0.022%-0.035% to chitosan oligosaccharide in the wherein said composition.
5. use the method for the composition for preventing and controlling tobacco mosaic that comprises chitosan oligosaccharide; It is characterized in that tobacco being used composition in the seedling stage of tobacco; Use the back first used 1 time in every interval 15-20 days again; Application times is 0-2 time once more, and the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.004%-0.01% in the composition of at every turn using that comprises chitosan oligosaccharide.
6. use the method for the composition for preventing and controlling tobacco mosaic that comprises chitosan oligosaccharide; It is characterized in that before the blooming of tobacco, tobacco being used composition; Use the back first used 1 time in every interval 15-20 days again; Application times is 0-2 time once more, and the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.004%-0.01% in the composition of at every turn using that comprises chitosan oligosaccharide.
7. use the method for the composition for preventing and controlling tobacco mosaic that comprises chitosan oligosaccharide; It is characterized in that after tobacco blooms, tobacco being used composition; Use the back first used 1 time in every interval 15-20 days again; Application times is 0-2 time once more, and the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.004%-0.01% in the composition of at every turn using that comprises chitosan oligosaccharide.
8. use the method for the composition for preventing and controlling tobacco mosaic that comprises chitosan oligosaccharide; It is characterized in that with said composition tobacco seed being handled, to account for the percentage of composition weight be 0.022%-0.035% to chitosan oligosaccharide in the wherein said composition, in the seedling stage of tobacco tobacco used composition then; Use the back first used 1 time in every interval 15-20 days again; Application times is 0-2 time once more, and the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.004%-0.01% in the composition of at every turn using that comprises chitosan oligosaccharide; Then at the preceding applying said compositions of blooming of tobacco; Use the back first and used 1 time in every interval 15-20 days again, application times is 0-2 time once more, and the concentration of at every turn using is identical or different with the concentration of using first; The percentage by weight of chitosan oligosaccharide is 0.004%-0.01% in the composition of at every turn using that comprises chitosan oligosaccharide; Applying said compositions after tobacco blooms is used the back first and was used 1 time in every interval 15-20 days again then, and application times is 0-2 time once more; The concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.004%-0.01% in the composition of at every turn using that comprises chitosan oligosaccharide.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103960092A (en) * | 2014-05-06 | 2014-08-06 | 云南省烟草公司玉溪市公司 | Three-dimensional biological control method for plant diseases and insect pests of tobacco planting |
| CN109362738A (en) * | 2018-12-21 | 2019-02-22 | 潍坊奥丰作物病害防治有限公司 | A kind of nicotine amino-oligosaccharide medicament and method for preventing and treating tobacco pest and disease damage |
| CN110089320A (en) * | 2019-04-11 | 2019-08-06 | 饶超奇 | A kind of application method and its application of chitosan solution |
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| CN1520723A (en) * | 2003-01-30 | 2004-08-18 | 中国科学院上海植物生理研究所 | Chitosan oligosaccharide containging pesticide composition and its application |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103960092A (en) * | 2014-05-06 | 2014-08-06 | 云南省烟草公司玉溪市公司 | Three-dimensional biological control method for plant diseases and insect pests of tobacco planting |
| CN103960092B (en) * | 2014-05-06 | 2015-09-16 | 云南省烟草公司玉溪市公司 | A kind of tobacco planting damage by disease and insect stereo biological prevention and controls |
| CN109362738A (en) * | 2018-12-21 | 2019-02-22 | 潍坊奥丰作物病害防治有限公司 | A kind of nicotine amino-oligosaccharide medicament and method for preventing and treating tobacco pest and disease damage |
| CN110089320A (en) * | 2019-04-11 | 2019-08-06 | 饶超奇 | A kind of application method and its application of chitosan solution |
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Application publication date: 20120328 |