CN102405906B - Chitooligo saccharide composition used for resisting pepper virus disease and application and method thereof - Google Patents
Chitooligo saccharide composition used for resisting pepper virus disease and application and method thereof Download PDFInfo
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- CN102405906B CN102405906B CN 201110332894 CN201110332894A CN102405906B CN 102405906 B CN102405906 B CN 102405906B CN 201110332894 CN201110332894 CN 201110332894 CN 201110332894 A CN201110332894 A CN 201110332894A CN 102405906 B CN102405906 B CN 102405906B
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Abstract
The invention relates to a chitooligo saccharide composiation used for resisting a pepper virus disease and an application and method thereof, and particularly relates to a pesticide composition used for resisting the pepper virus disease. The pesticide composition comprises the chitooligo saccharide, wherein the chitooligo saccharide is 0.005%-0.025% of the pesticide composition in percentage byweight. The invention also provides an application of the pesticide composition containing the chitooligo saccharide used for preventing the pepper virus disease, the application is characterized in that the chitooligo saccharide is 0.005-0.025% of the pesticide composition containing the chitooligo saccharide by weight. The composition provided by the invention solves an important problem of resisting pepper virus disease, in particular the pesticide composition comprising the chitooligo saccharide is used for processing seeds and plants of pepper so as to not only resist the pepper virus disease but also generate no phytotoxicity on crops, thereby having no pesticide residue.
Description
Technical field
The invention belongs to agrochemical field, particularly relate to the control composition of pepper virus disease and prevent and treat method, relate to particularly a kind of chitosan oligosaccharide composition and use thereof and method for anti-pepper virus disease.
Background technology
Pepper (Piper nigrum Linn.) is the evergreen climbing plants in a kind of torrid zone, also is a kind of important tropical fragrant hot plant, no matter planting scale, output and value all occupy the first in all spices, is called as " king of spices " and " coal ".Worldwide, (claim again root rot (foot rot) except the pepper seasonal febrile diseases at present, phytophthora root rot (Phytophthora foot rot), speed rot (quick wilt), speed dead sick (sudden death), cause of disease is soil fungi Phytophthora capsici (Phytophthora capsici)) and pepper slow-decline (slow wilt) (claim again (delaying) chronic weak or decline disease (slow decline), or Yellowing Disease of Black (pepper yellows), cause of disease is cavity nematode (Radopholus similis), root-knot nematode (Meloidogyne incognita) and Phytophthora capsici (Phytophthora capsici)) for outside the Major Diseases that endangers pepper production, the pepper virus disease shows more and more serious trend in recent years.This situation must cause pepper grower and researcher's attention.The kind of pepper virus disease mainly contains pepper mosaic disease, pepper piebald disease, pepper phyllody etc.
The at present control for the pepper virus disease does not have good chemical agent available, and general expression is destroyed diseased plant or prevented and treated the measure such as viral insect carrier.
The present invention is surprised to find that chitosan oligosaccharide can prevent the pepper virus disease effectively, and has designed chitosan oligosaccharide composition, purposes and application process for this disease in the control for the pepper virus disease, can play good effect to the pepper virus disease.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of composition pesticide for anti-pepper virus disease, comprise chitosan oligosaccharide in this composition pesticide, the percentage by weight that chitosan oligosaccharide accounts for this composition pesticide is 0.005%-0.025%.Preferably, to account for the percentage by weight of this composition pesticide be 0.012%-0.025% or 0.005%-0.01% to chitosan oligosaccharide.
In the above-mentioned composition, the pepper virus disease is the disease that any virus that can produce on the pepper causes, and the chitosan oligosaccharide composition shows to the control of pepper virus disease and on the whole control be separately every kind of virus disease of control that its preventive effect is highly significant also.The pepper virus disease includes but not limited to pepper mosaic disease, pepper piebald disease, pepper phyllody etc.
Above-mentioned this composition pesticide can prepare liquid preparation or solid pharmaceutical preparation.Liquid preparation can be any liquid preparation of agriculture usefulness, and solid pharmaceutical preparation also is any solid pharmaceutical preparation of agriculture usefulness.Preferably, liquid preparation is aqua, microemulsion, water dispersible granules or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or granule.
The present invention also provides the above-mentioned composition pesticide that comprises chitosan oligosaccharide to be used for the purposes of control pepper virus disease, the percentage by weight that it is characterized in that comprising chitosan oligosaccharide in the composition pesticide of chitosan oligosaccharide is 0.005%-0.025%, preferably, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.005%-0.01%, perhaps preferred, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.012%-0.025%.
The present invention also provides the method for using the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that the stages set of applications compound that pepper grows is processed pepper, comprise the pepper seed is processed, perhaps use composition seedling stage in pepper, perhaps use composition flowering stage in pepper, perhaps after blooming, pepper uses composition, perhaps to the pepper seed, seedling stage, before blooming, use successively composition after blooming, the percentage by weight of chitosan oligosaccharide is 0.005%-0.025% in the wherein said composition, preferably, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.005%-0.01%, perhaps preferred, the percentage by weight that chitosan oligosaccharide accounts for composition is 0.012%-0.025%.
In the said method, described composition can prepare liquid preparation or solid pharmaceutical preparation.Liquid preparation can be any liquid preparation of agriculture usefulness, and solid pharmaceutical preparation also is any solid pharmaceutical preparation of agriculture usefulness.Preferably, liquid preparation is aqua, microemulsion, water dispersible granules or suspending agent; Solid pharmaceutical preparation is preferably wetting powder or granule.
The present invention also provides the method for using the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that the pepper seed is processed, to account for the percentage of composition weight be 0.01%-0.03% to chitosan oligosaccharide in the wherein said composition, preferably, to account for the percentage of composition weight be 0.012%-0.025% to chitosan oligosaccharide.The method of wherein the pepper seed being processed can be the method for treating seeds of this area routine, includes but not limited to seed dressing, dipping, seed soaking or vexed kind.Wherein seed dressing refers to medicinal powder or liquid and seed are mixed; Dipping be medicine liquid spray on seed, and constantly stir and make seed all wetting, cover the coverings such as mat, pile vexed rear sowing; Seed soaking refers to that a seed or seedling are immersed in the certain density liquid, makes seed or seedling absorb medicament through certain hour; Vexed kind refers to liquid and seed are mixed the method that the vexed certain hour of rear heap is sowed again.
The present invention also provides the method for using the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that in the seedling stage of pepper pepper being used composition, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.005%-0.01% in the composition that comprises chitosan oligosaccharide of at every turn using.
The present invention also provides the method for using the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that before the blooming of pepper, pepper being used composition, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.005%-0.01% in the composition that comprises chitosan oligosaccharide of at every turn using.
The present invention also provides the method for using the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that after pepper blooms, pepper being used composition, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.005%-0.01% in the composition that comprises chitosan oligosaccharide of at every turn using.
The present invention also provides the method for using the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that with described composition the pepper seed being processed, to account for the percentage of composition weight be 0.012%-0.025% to chitosan oligosaccharide in the wherein said composition, then in the seedling stage of pepper pepper is used composition, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, the percentage by weight of chitosan oligosaccharide is 0.005%-0.01% in the composition that comprises chitosan oligosaccharide of at every turn using, then applying said compositions before the blooming of pepper, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, the percentage by weight of chitosan oligosaccharide is 0.005%-0.01% in the composition that comprises chitosan oligosaccharide of at every turn using, then applying said compositions after pepper blooms, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.005%-0.01% in the composition that comprises chitosan oligosaccharide of at every turn using.
In the said method, the method for wherein the pepper seed being processed can be the method for treating seeds of this area routine, includes but not limited to seed dressing, dipping, seed soaking or vexed kind.Wherein seed dressing refers to medicinal powder or liquid and seed are mixed; Dipping be medicine liquid spray on seed, and constantly stir and make seed all wetting, cover the coverings such as mat, pile vexed rear sowing; Seed soaking refers to that a seed or seedling are immersed in the certain density liquid, makes seed or seedling absorb medicament through certain hour; Vexed kind refers to liquid and seed are mixed the method that the vexed certain hour of rear heap is sowed again.
In the said method, any method of effectively commonly using the when method of using refers to the applications of pesticide includes but not limited to powder injection process, spray-on process, fumigating system, soil treatment method or smog method etc.
Wherein powder injection process refers to that the wind-force that utilizes duster to produce is sprinkling upon the agricultural chemicals of pulvis the surface of crop or controlling object equably; Spray-on process refers to be made into liquid with a certain amount of agricultural chemicals and an amount of water, with the spraying apparatus medicine liquid spray is become droplet, is evenly distributed in crop or controlling object surface; Fumigating system refers to adopt fumigant or volatile medicament, makes its volatilization become the effectively gas of prevention and elimination of disease and pests; The soil treatment method refers to take spray, dusts, spreads the methods such as pesticide-clay mixture, and agricultural chemicals is imposed in ground or the certain soil layer equably; The smog method refers to utilize special-purpose facility that the oily pesticide dispersion is become smoke condition, to reach the method for Pesticidal and sterilizing weeding.
In the said method, with composition seed is processed, referred to composition is mixed with seed, composition is in the situation of liquid, and seed is soaked in the liquid, when composition is solid pharmaceutical preparation, seed is mixed with solid pharmaceutical preparation.
Among the present invention, chitosan oligosaccharide is that the degree of polymerization that Glucosamine is formed by connecting by Isosorbide-5-Nitrae-glycosidic bond is the mixture of the high molecular polymer of 2-30, and mean molecule quantity is 1000-3000 dalton.Chitosan oligosaccharide can prepare by any means, and such as following method: deacetylation is the mixture of the chitosan oligomer of 2-30 at the shitosan more than 70% by the degree of polymerization that the methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation method prepare.Perhaps, be the mixture of the chitosan oligomer of 2-30 by from fungal cell wall or insect body wall, extracting the degree of polymerization that obtains chitin and prepare by methods such as mechanical degradation method, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, composite degradation methods.Perhaps, by being the mixture of the polymer of 2-30 by Glucosamine by the degree of polymerization that Isosorbide-5-Nitrae-the glycosidic bond chemosynthesis is polymerized.Chitosan oligosaccharide is the mixture of the high molecular polymer of different polymer at present, that is to say in the chitosan oligosaccharide to be not the oligosaccharides form of the single degree of polymerization, but the chitosan oligosaccharide monose of every kind of degree of polymerization may exist all.So no matter by which kind of method prepare; the commercially available prod that perhaps prepares by above-mentioned preparation method; as long as it is that Glucosamine passes through 1; the degree of polymerization that the 4-glycosidic bond is formed by connecting is that 2-30, mean molecule quantity are that the mixture of the daltonian high molecular polymer of 1000-3000 is exactly chitosan oligosaccharide of the present invention; it can contain acetyl group; but the sugar unit of bonding acetyl group is no more than 40% amount, preferably is no more than 30% amount, especially preferably is no more than 20% amount.Commercially available chitosan oligosaccharide all is the chitosan oligosaccharide product that above-mentioned chitosan oligosaccharide term is set forth basically at present.
Above-mentioned composition of the present invention and method can reduce or eliminate the symptom that all virus diseases can cause in performance in control pepper virus disease.For example, the symptom of the virus disease of different kinds is as follows:
The pepper mosaic disease symptom of catching an illness is that spire and climax leaves vein are limpid transparent, and yellow spotting appears in blade, and blade diminishes, stenosis, and crumple, curling, lopsided, frangible comes off before the blade maturation usually, and remaining blade edge is downright bad, and leaf texture is hard and frangible.The serious susceptible climing hypoevolutism of stem, plant is short and small, and Hua Sui is short, and mostly comes off before setting, and residual fruit ear is short and the result is few, and fruit is also little, causes production declining.Because internode is unusually short, causes whole strain undergrowth, infected branch shows typical witch's broom feature.It is also downright bad less to absorb root, and the cortical tissue of the climing part of subterranean stem thickens and is crineous.The stem that infects is climing to the fertilising Low Response.Infection in various degree shows above-mentioned partial symptoms, and the slightest symptom only flower leaf paresthesia occurs at blade, and vine growth and development is normal, and output is also acted normally.Sometimes climing to show a part of branches and leaves normal for the stem that infects of part, a part of branches and leaves performance symptom of catching an illness.Virus disease is propagated slower, and seldom can cause death, but can make the climing continuous downturn of stem and make yield reducation even total crop failure.
Pepper maculopathy classical symptom is that vein is limpid, and blade scatters bright chlorisis spot, causes at last bright chlorisis between vein.On some kinds, can make vein overstriking, show bar band shape after catching an illness, Lutao shape symptom appears in blade.Other symptom comprises that blade diminishes, curling, distortion, lopsided, frangible, and has the chlorisis spot between vein.Chlorisis spot or macula lutea badly influence the formation of fruit ear and fruit when intensive.Internode is unusually short, causes whole strain undergrowth.The climing vigor of the stem of catching an illness and yield reducation, and continuous downturn.
The sick infected stem cissoid sheet yellow of pepper phyllody or phyllody, diminish, blade and hypogenetic branch form bunch shape, present typical witch's broom feature.The branch internode shortens, and the climing as a result side shoot of susceptible stem is very short and small, and fruit ear is out of shape to some extent.Bud changes into foliation structure, often grows small fruit bearing shoot.The infection fruit ear is elongated, and fruit is oblong or not round.Plant is sterile in the serious situation, total crop failure.The very fast decline of the climing meeting of infected stem and death.
Beneficial effect of the present invention:
The present invention has found to be used for the composition pesticide that comprises chitosan oligosaccharide of anti-pepper virus disease, this composition pesticide can resist the pepper virus disease, particularly found the special effect of chitosan oligosaccharide when anti-pepper virus disease of certain concentration, solve the major issue of anti-pepper virus disease, especially use the composition pesticide that comprises chitosan oligosaccharide behind the seed and plant of processing pepper, anti-pepper virus disease not only, and can be to not doing the deposits yields poisoning, do not have residue of pesticide, realized the control of the pepper virus disease of environment protection-type.The special contribution of the present invention also is to have found to use good chance and the concentration that chitosan oligosaccharide is prevented and treated the pepper virus disease by research, has greatly improved the effect of chitosan oligosaccharide control pepper virus disease.
Embodiment
In order to understand the present invention, the below further specifies the present invention with embodiment, but does not limit the present invention.
Embodiment 1:
5wt% chitosan oligosaccharide solution with water is diluted to respectively corresponding application concentration, concentration is calculated according to weight percent concentration, calculate with ppm (1,000,000/) concentration in outcome record, what 50ppm represented in the outcome record below is that weight percent concentration is 0.005%.Clear water is processed in contrast.According to demand treatment by stages pepper seed and pepper plant, seed treatment is that seed soaks with the solution after the dilution, and soak time is 24 hours.The processing mode in other stage is that the chitosan oligosaccharide aqua sprays processing, and be 15 days blanking time that sprays processing.Each processing is observed and is detected by 3 experimental field (667 square metres), adds up when the sample of processing seed grows up to 4 leaves after crop germinates, and the statistics of processing for the stages in vegetative period was added up after dispenser in 7 days.Add up respectively the percentage of the pepper diseased plant generation of every experimental field, just its plant is defined as the virus disease plant as long as produced the feature of virus disease.Concrete disposition and effect are as follows:
Table 1 seed immersion treatment is to the control efficiency of pepper virus disease
Table 2 seedling spraying is to the control efficiency of pepper virus disease
Before blooming, table 3 sprays the control efficiency to the pepper virus disease
After blooming, table 4 sprays the control efficiency to the pepper virus disease
Table 5 is omnidistance processes pepper to the control efficiency of pepper virus disease
No matter show by above-mentioned experiment, be to process, bloom pre-treatment or bloom post processing in treatment by stages pepper seed, seedling stage, and chitosan oligosaccharide solution has all shown good anti-pepper virus disease effect.All use chitosan oligosaccharide solution each period in pepper, show especially the preventive effect of good anti-pepper virus disease.
Claims (3)
1. use the method for the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that before the blooming of pepper, pepper being used composition, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.005%-0.01% in the composition that comprises chitosan oligosaccharide of at every turn using.
2. use the method for the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that after pepper blooms, pepper being used composition, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.005%-0.01% in the composition that comprises chitosan oligosaccharide of at every turn using.
3. use the method for the composition for preventing and controlling pepper virus disease that comprises chitosan oligosaccharide, it is characterized in that with described composition the pepper seed being processed, to account for the percentage of composition weight be 0.012%-0.025% to chitosan oligosaccharide in the wherein said composition, then in the seedling stage of pepper pepper is used composition, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, the percentage by weight of chitosan oligosaccharide is 0.005%-0.01% in the composition that comprises chitosan oligosaccharide of at every turn using, then applying said compositions before the blooming of pepper, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, the percentage by weight of chitosan oligosaccharide is 0.005%-0.01% in the composition that comprises chitosan oligosaccharide of at every turn using, then applying said compositions after pepper blooms, use first and used again 1 time in rear every interval 15-20 days, application times is 0-2 time again, the concentration of at every turn using is identical or different with the concentration of using first, and the percentage by weight of chitosan oligosaccharide is 0.005%-0.01% in the composition that comprises chitosan oligosaccharide of at every turn using.
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| CN1316882C (en) * | 2003-01-30 | 2007-05-23 | 中国科学院上海生命科学研究院 | Chitosan oligosaccharide containging pesticide composition and its application |
| US7070778B2 (en) * | 2003-10-30 | 2006-07-04 | Laboratoire Goemar Sa | Therapeutical combination against cancer |
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| 2%氨基寡糖素防治几种作物病毒病田间药效试验;赵小明等;《第二届全国绿色环保农药新技术、新产品交流会》;20031101;第164-166页，参见第164页1.4节、第166页表3 * |
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| 赵小明等.2%氨基寡糖素防治几种作物病毒病田间药效试验.《第二届全国绿色环保农药新技术、新产品交流会》.2003,参见第164页1.4节、第166页表3. |
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