CN105076168A - Pesticide composition containing albendazole and fluopyram - Google Patents
Pesticide composition containing albendazole and fluopyram Download PDFInfo
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- CN105076168A CN105076168A CN201510560033.6A CN201510560033A CN105076168A CN 105076168 A CN105076168 A CN 105076168A CN 201510560033 A CN201510560033 A CN 201510560033A CN 105076168 A CN105076168 A CN 105076168A
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Abstract
The invention discloses a pesticide composition containing albendazole and fluopyram. The pesticide composition comprises a first effective ingredient albendazole, a second effective ingredient fluopyram and common auxiliary ingredients, wherein the weight ratio of albendazole to fluopyram is (30:1)-(1:20). Results of lots of indoor and outdoor tests show that the pesticide composition provided by the invention has an obvious synergistic effect, and is good in fast-acting property and long in period of preserving effects, the control effect of the pesticide composition provided by the invention on crop nematode is obviously superior to that of a single pesticide used separately, meanwhile, pesticide dosage is reduced, the pesticide residues on crops are reduced, environmental pollution is reduced, pesticide resistance of nematode is delayed and the service life of the pesticide is prolonged.
Description
Technical field
The present invention relates to composition pesticide and application thereof, specifically a kind of composition pesticide that is active ingredient with rosickyite azoles and fluorine pyrrole bacterium acid amides, for preventing and treating crops nematode.
Background technology
Rosickyite azoles (albendazole) is benzimidazole insecticidal bactericide.As medicine, can be used for ascarifuge, pinworm, tapeworm, whipworm, hookworm, excrement strongylid etc., metabolism is after sulfoxide type or sulfone class in vivo, suppress parasite to the absorption of glucose, cause polypide glycogen depletion, or suppress fumaric reductase system, hinder the generation of ATP, parasite cannot be survived and breed.Meanwhile, good control efficiency is also had to the nematode on plant.
Fluorine pyrrole bacterium acid amides (fluopyram), it is a kind of new benzamides series bactericidal agent, mitochondrial respiratory is suppressed by hindering the electro transfer of succinate dehydrogenase in respiratory chain, can the crop diseases that caused by fungal pathogen of prevention and control, comprise fruit, vegetables, potato and flowers all have application in 70 interior various crop, meanwhile, it also has good control efficiency to the nematode on plant.
Plant nematode is the very harmful pathogenic microorganism of a class, it has a very wide distribution, of a great variety, adaptive capacity to environment is strong, can colonize in the root system of plant, stem, leaf, seed, young shoot and fruit, by feeding activity, mechanical damage is caused to host plant, and rob the nutrition of host plant, especially its esophageal gland secretion can cause host plant that a series of pathology occurs, and also jointly can infect host plant with other pathogenic microorganism, cause compound disease, cause production loss.In the world, the agricultural losses that plant nematode is caused every year, up to more than 1,000 hundred million dollars, seriously constrain the development of agricultural economy.
The compounded formula screening of the present inventor to rosickyite azoles and fluorine pyrrole bacterium acid amides conducts in-depth research, find that rosickyite azoles and fluorine pyrrole bacterium acid amides carry out composite, within the scope of certain compound proportion, to Plant nematode, there is significant synergistic function, after further study, complete the present invention.
Summary of the invention
The object of the present invention is to provide a kind of composition pesticide containing rosickyite azoles and fluorine pyrrole bacterium acid amides, be applied to the control of crops nematode.
Technical solution of the present invention: a kind of composition pesticide containing rosickyite azoles and fluorine pyrrole bacterium acid amides, active ingredient is rosickyite azoles and fluorine pyrrole bacterium acid amides, the weight ratio of rosickyite azoles and fluorine pyrrole bacterium acid amides is 30:1 ~ 1:20, preferred weight ratio is 10:1 ~ 1:5, rosickyite azoles and fluorine pyrrole bacterium acid amides gross weight percentage composition are in the composition 0.1% ~ 80%, and all the other are allow in agricultural chemicals to use and acceptable auxiliary element.
Described composition pesticide can according to well known to a person skilled in the art method, be mixed with the application forms of the applicable agricultural uses such as microcapsule suspending agent, suspending agent, aqueous emulsion, microemulsion, water dispersible granules, wetting powder, granule and missible oil, the production technology of various application forms all belongs to existing known technology, does not repeat them here.
Described auxiliary element is solvent, emulsifier, wetting agent, dispersant, stabilizing agent, antifreezing agent, thickener, binding agent, disintegrant, defoamer, bleeding agent, macromolecule cyst material, synergist, film forming agent, preservative and filler etc., it is all composition that is conventional in pesticidal preparations or permission use, be not particularly limited, concrete composition and consumption are determined by simple test according to recipe requirements.
Composition pesticide provided by the invention, the method use usually adopting spraying, soak seed or spread fertilizer over the fields, also can adopt other operation techniques agriculturally applied as required.
Compared with prior art, the invention has the advantages that:
1, rosickyite azoles and fluorine pyrrole bacterium acid amides is composite has synergistic function, is used alone the control efficiency significantly improved nematode than single dose.
2, rosickyite azoles and fluorine pyrrole bacterium acid amides two kinds of active ingredients composite, decrease Pesticide use amount, reduce use cost, alleviate pollution to environment.
3, rosickyite azoles and fluorine pyrrole bacterium acid amides two kinds of active ingredients composite, the mechanism of action is different, service life of having delayed the drug-fast generation of nematode, having added agricultural chemicals.
Embodiment
In order to understand essence of the present invention better; below in conjunction with embodiment, content of the present invention is described further; but can not limitation of the present invention be considered as; the following stated is only for explaining the present invention; for not departing from amendment that the present invention's spirit and principle make, replacement or improvement, all belong to the scope of protection of present invention.
Embodiment one: 30% rosickyite azoles fluorine pyrrole bacterium acid amides microcapsule suspending agent.
Following material is all weight percentage: rosickyite azoles 25%, fluorine pyrrole bacterium acid amides 5%, tolyl isocyanate 10%, trimethylbenzene 10%, ethyl acetate 10%, castor oil polyoxyethylene ether 5%, calcium lignosulfonate 3%, sodium hydroxide 2%, deionized water complements to 100%.By above-mentioned active ingredient, macromolecule cyst material, solvent, make to be dissolved into homogeneous oil phase, in a shear condition, oil phase is joined in the aqueous phase solution containing emulsifier, dispersant, pH adjusting agent, surplus deionized water is supplied, bi-material reacts at oil-water interfaces, forms macromolecule cyst wall, can obtain 30% rosickyite azoles fluorine pyrrole bacterium acid amides microcapsule suspending agent.
This embodiment is for preventing and treating cucumber root-knot nematode: 30% rosickyite azoles fluorine pyrrole bacterium acid amides microcapsule suspending agent is by 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to cucumber root-knot nematode is respectively 98.47%, 96.39%, 92.68%; 10% rosickyite azole suspending agent presses 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to cucumber root-knot nematode is respectively 68.96%, 64.37%, 55.24%; 20% fluorine pyrrole bacterium amide suspending agents presses 150ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to cucumber root-knot nematode is respectively 75.87%, 71.29%, 64.91%.Visible, obvious synergy after rosickyite azoles and fluorine pyrrole bacterium acid amides binary built, quick-acting is good, lasting period is long, the preventive effect of cucumber root-knot nematode is used alone significantly better than each single dose, meanwhile, in process of the test, does not find that this binary built medicament produces poisoning to cucumber.
Embodiment two: 20% rosickyite azoles fluorine pyrrole bacterium amide suspending agents.
Following material is all weight percentage: rosickyite azoles 10%, fluorine pyrrole bacterium acid amides 10%, dodecyl sodium sulfate 10%, triphen ethyl phenol APEO and phosphate 5%, polycarboxylate 5%, propane diols 3%, xanthans 1%, silicone oil 0.1%, deionized water supplies 100%.By above-mentioned active ingredient, wetting agent, dispersant, antifreezing agent, thickener and defoamer sand milling in high speed shear dispersion, sand mill, the high dispersive formed in water-medium, stable suspension system, can obtain 20% rosickyite azoles fluorine pyrrole bacterium amide suspending agents.
This embodiment is for preventing and treating watermelon root-knot: 20% rosickyite azoles fluorine pyrrole bacterium amide suspending agents is by 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to watermelon root-knot is respectively 96.92%, 93.17%, 90.84%; 10% rosickyite azole suspending agent presses 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to watermelon root-knot is respectively 71.42%, 64.37%, 59.23%; 20% fluorine pyrrole bacterium amide suspending agents presses 150ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to watermelon root-knot is respectively 73.91%, 68.45%, 63.34%.Visible, obvious synergy after rosickyite azoles and fluorine pyrrole bacterium acid amides binary built, quick-acting is good, lasting period is long, the preventive effect of watermelon root-knot is used alone significantly better than each single dose, meanwhile, in process of the test, does not find that this binary built medicament produces poisoning to watermelon.
Embodiment three: 45% rosickyite azoles fluorine pyrrole bacterium acid amides aqueous emulsion.
Following material is all weight percentage: rosickyite azoles 25%, fluorine pyrrole bacterium acid amides 20%, trimethylbenzene 10%, cyclohexanone 10%, polyoxyethylene nonylphenol ether 15%, sodium lignin sulfonate 3%, xylitol 1%, glycerine 1%, deionized water supplies 100%.Above-mentioned active ingredient, solvent, emulsifier, dispersant and stabilizing agent are mixed to form oil phase, antifreezing agent dissolves and forms aqueous phase in water, slowly oil phase is added in aqueous phase under clipper effect, and continue to shear about 30 minutes, the rosickyite azoles fluorine pyrrole bacterium acid amides aqueous emulsion of obtained 45%.
This embodiment is for preventing and treating banana root-knot nematode: the rosickyite azoles fluorine pyrrole bacterium acid amides aqueous emulsion of 45% is by 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to banana root-knot nematode is respectively 97.23%, 94.16%, 92.76%; 10% rosickyite azole suspending agent presses 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to banana root-knot nematode is respectively 70.32%, 64.17%, 58.54%; 20% fluorine pyrrole bacterium amide suspending agents presses 150ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to banana root-knot nematode is respectively 76.92%, 71.35%, 67.83%.Visible, obvious synergy after rosickyite azoles and fluorine pyrrole bacterium acid amides binary built, quick-acting is good, lasting period is long, the preventive effect of banana root-knot nematode is used alone significantly better than each single dose, meanwhile, in process of the test, does not find that this binary built medicament produces poisoning to banana.
Embodiment four: 40% rosickyite azoles fluorine pyrrole bacterium acid amides microemulsion.
Following material is all weight percentage: rosickyite azoles 30%, fluorine pyrrole bacterium acid amides 10%, trimethylbenzene 10%, cyclohexanone 10%, Triton X-100 10%, tributyl phosphate 2%, thiophene ketone 1%, propyl isome 2%, deionized water supplies 100%.Active ingredient solvent is dissolved completely, and then add emulsifier, stabilizing agent, bleeding agent, synergist mix, finally add deionized water, after fully stirring, the rosickyite azoles fluorine pyrrole bacterium acid amides microemulsion of 40% can be obtained.
This embodiment is for preventing and treating capsicum root-knot nematode: the rosickyite azoles fluorine pyrrole bacterium acid amides microemulsion of 40% is by 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to capsicum root-knot nematode is respectively 98.51%, 94.79%, 91.64%; 10% rosickyite azole suspending agent presses 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to capsicum root-knot nematode is respectively 69.26%, 64.74%, 57.36%; 20% fluorine pyrrole bacterium amide suspending agents presses 150ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to capsicum root-knot nematode is respectively 75.12%, 70.92%, 63.31%.Visible, obvious synergy after rosickyite azoles and fluorine pyrrole bacterium acid amides binary built, quick-acting is good, lasting period is long, the preventive effect of capsicum root-knot nematode is used alone significantly better than each single dose, meanwhile, in process of the test, does not find that this binary built medicament produces poisoning to capsicum.
Embodiment five: 40% rosickyite azoles fluorine pyrrole bacterium acid amides water dispersible granules.
Following material is all weight percentage: rosickyite azoles 25%, fluorine pyrrole bacterium acid amides 15%, sulfosuccinic monooctyl ester sodium salt 10%, alkylphenol polyoxyethylene 10%, acrylate homopolymer sodium salt 5%, urea 2%, attapulgite supplies 100%.Above-mentioned active ingredient, wetting agent, dispersant, disintegrant and filler are mixed, pulverize with micro jet, through mediating, then adding in fluidized-bed granulation dryer and carrying out granulation, drying, screening, 40% rosickyite azoles fluorine pyrrole bacterium acid amides water dispersible granules can be obtained.
This embodiment is for preventing and treating tomato root-knot eelworm: 40% rosickyite azoles fluorine pyrrole bacterium acid amides water dispersible granules is by 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to tomato root-knot eelworm is respectively 97.87%, 95.58%, 92.13%; 10% rosickyite azole suspending agent presses 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to tomato root-knot eelworm is respectively 71.06%, 63.64%, 54.98%; 20% fluorine pyrrole bacterium amide suspending agents presses 150ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to tomato root-knot eelworm is respectively 73.67%, 67.84%, 62.39%.Visible, obvious synergy after rosickyite azoles and fluorine pyrrole bacterium acid amides binary built, quick-acting is good, lasting period is long, the preventive effect of tomato root-knot eelworm is used alone significantly better than each single dose, meanwhile, in process of the test, does not find that this binary built medicament produces poisoning to tomato.
Embodiment six: 60% rosickyite azoles fluorine pyrrole bacterium acid amides wetting powder.
Following material is all weight percentage: rosickyite azoles 50%, fluorine pyrrole bacterium acid amides 10%, fatty alcohol-polyoxyethylene ether 10%, acrylate homopolymer sodium salt 10%, potter's clay supplies 100%.By above-mentioned, active ingredient, wetting agent, dispersant and filler are mixed, again through air-flow crushing after mechanical crushing, mix, 60% rosickyite azoles fluorine pyrrole bacterium acid amides wetting powder can be obtained.
This embodiment is for preventing and treating root knot nematode in tobacco: 60% rosickyite azoles fluorine pyrrole bacterium acid amides wetting powder is by 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to root knot nematode in tobacco is respectively 97.62%, 93.58%, 91.37%; 10% rosickyite azole suspending agent presses 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to root knot nematode in tobacco is respectively 69.84%, 61.21%, 53.96%; 20% fluorine pyrrole bacterium amide suspending agents presses 150ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to root knot nematode in tobacco is respectively 76.93%, 70.35%, 65.87%.Visible, obvious synergy after rosickyite azoles and fluorine pyrrole bacterium acid amides binary built, quick-acting is good, lasting period is long, the preventive effect of root knot nematode in tobacco is used alone significantly better than each single dose, meanwhile, in process of the test, does not find that this binary built medicament produces poisoning to tobacco.
Embodiment seven: 5% rosickyite azoles fluorine pyrrole bacterium acid amides granule.
Following material is all weight percentage: rosickyite azoles 4%, fluorine pyrrole bacterium acid amides 1%, lauryl sodium sulfate 10%, alkylphenol polyoxyethylene 15%, polyvinyl alcohol 20%, kaolin complements to 100%.Above-mentioned active ingredient, wetting agent, dispersant, binding agent and filler are mediated through mixing and water adding, uses pinch-out granulator pinch out and be shaped, afterwards by particle fluid-bed drying, screening, 5% rosickyite azoles fluorine pyrrole bacterium acid amides granule can be obtained.
This embodiment is for preventing and treating peanut root-knot nematode: 5% rosickyite azoles fluorine pyrrole bacterium acid amides granule spreads fertilizer over the fields process by 250ga.i./ha, and after medicine, 3d, 7d, 20d preventive effect to peanut root-knot nematode is respectively 95.26%, 93.49%, 90.13%; 10% rosickyite azole suspending agent presses 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to peanut root-knot nematode is respectively 71.74%, 64.27%, 57.32%; 20% fluorine pyrrole bacterium amide suspending agents presses 150ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to peanut root-knot nematode is respectively 76.61%, 71.44%, 64.56%.Visible, obvious synergy after rosickyite azoles and fluorine pyrrole bacterium acid amides binary built, quick-acting is good, lasting period is long, the preventive effect of peanut root-knot nematode is used alone significantly better than each single dose, meanwhile, in process of the test, does not find that this binary built medicament produces poisoning to peanut.
Embodiment eight: 40% rosickyite azoles fluorine pyrrole bacterium acid amides missible oil.
Following material is all weight percentage: rosickyite azoles 24%, fluorine pyrrole bacterium acid amides 16%, trimethylbenzene 10%, cyclohexanone 10%, styrenated phenol APEO 20%, double to kill 3%, and solvent naphtha complements to 100%.Fully dissolve after above-mentioned active ingredient is added solvent, then add emulsifier, synergist becomes transparent and homogeneous oily liquids after stirring, 40% rosickyite azoles fluorine pyrrole bacterium acid amides missible oil can be obtained.
This embodiment is for preventing and treating ginger root-knot nematode: 40% rosickyite azoles fluorine pyrrole bacterium acid amides missible oil is by 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to ginger root-knot nematode is respectively 97.12%, 94.68%, 92.23%; 10% rosickyite azole suspending agent presses 250ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to ginger root-knot nematode is respectively 72.05%, 64.56%, 56.91%; 20% fluorine pyrrole bacterium amide suspending agents presses 150ga.i./ha thin up root irrigation, and after medicine, 3d, 7d, 20d preventive effect to ginger root-knot nematode is respectively 74.59%, 69.43%, 62.67%.Visible, obvious synergy after rosickyite azoles and fluorine pyrrole bacterium acid amides binary built, quick-acting is good, lasting period is long, the preventive effect of ginger root-knot nematode is used alone significantly better than each single dose, meanwhile, in process of the test, does not find that this binary built medicament produces poisoning to ginger.
Embodiment nine: Toxicity Determination is tested.
In order to understand with rosickyite azoles and fluorine pyrrole bacterium acid amides for the composite synergy type of active ingredient further, present inventor has performed a large amount of Toxicity Determination tests.
1, rosickyite azoles and fluorine pyrrole bacterium acid amides are to the indoor joint toxicity measuring of cucumber root-knot nematode.
Test method: first get the cucumber root producing a large amount of root knot, rinse well gently with running water, then with the milky egg capsule on dissecting needle gently picking old complaint, put into the little culture dish of diameter 6cm, add a small amount of sterile water, in 25 DEG C of insulating boxs, hatch 3 ~ 4d, collect 2 instar larvaes, and adding sterile water, to be mixed with the suspension of finite concentration (500/about ml) for subsequent use.
Setting 5 concentration gradient process, take sterile water as blank.Get 96 porocyte culture plates, every hole adds medicament 150 microlitre of the variable concentrations prepared, add isopyknic nematode suspension again, under 25 DEG C of conditions, moisturizing is cultivated, the amount of survival of 48h microscopy nematode and dead quantity after treatment, nematode is stiff motionless for dead worm, and nematode is the worm that lives in bending wriggling state.Calculate lethality and corrected mortality.
Carry out statistical analysis with DPS statistical analysis software, calculate the LC of each medicament
50, and the co-toxicity coefficient (CTC value) of mixture is calculated according to the abundant method of Sun Yun.
Actual measurement toxicity index (ATI)=(standard agent LC
50/ reagent agent LC
50) × 100.
In theoretical toxicity index (TTI)=A medicament toxicity index × mixture A percentage composition+B medicament toxicity index × mixture in the percentage composition of B.
Co-toxicity coefficient (CTC)=[the theoretical toxicity index (TTI) of mixture actual measurement toxicity index (ATI)/mixture] × 100.
According to the NY/T11547.7-2006 insecticide combination effect criteria for classifying: co-toxicity coefficient (CTC) >=120 shows as synergistic effect; Co-toxicity coefficient (CTC)≤80 shows as antagonism; 80 < co-toxicity coefficient (CTC) < 120 show as summation action.
Table 1, rosickyite azoles and fluorine pyrrole bacterium acid amides are to the indoor joint toxicity measuring result of cucumber root-knot nematode
| Medicament title and proportioning (weight ratio) | LC 50(mg/L) | ATI | TTI | CTC |
| Rosickyite azoles | 12.94 | 100.00 | - | - |
| Fluorine pyrrole bacterium acid amides | 8.15 | 158.77 | - | - |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (30:1) | 7.68 | 168.49 | 101.90 | 165.35 |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (20:1) | 6.82 | 189.74 | 102.80 | 184.57 |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (10:1) | 5.69 | 227.42 | 105.34 | 215.89 |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (5:1) | 4.73 | 273.57 | 109.80 | 249.15 |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (1:1) | 4.19 | 308.83 | 129.39 | 238.68 |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (1:5) | 4.26 | 303.76 | 148.98 | 203.89 |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (1:10) | 4.91 | 263.54 | 153.43 | 171.77 |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (1:20) | 5.43 | 238.31 | 155.97 | 152.79 |
As can be seen from Table 1, rosickyite azoles and fluorine pyrrole bacterium acid amides composite in the scope of 30:1 ~ 1:20 (by weight) time, co-toxicity coefficient (CTC) is all greater than 120, all shows synergistic function to cucumber root-knot nematode; Rosickyite azoles and fluorine pyrrole bacterium acid amides are in the scope of 10:1 ~ 1:5 (by weight), and synergistic function is more remarkable, and co-toxicity coefficient (CTC) is all more than 200; Rosickyite azoles and fluorine pyrrole bacterium acid amides composite with 5:1 (by weight), synergistic function is the most remarkable, and co-toxicity coefficient (CTC) is 249.15.Visible, rosickyite azoles and fluorine pyrrole bacterium acid amides is composite has reasonability and feasibility.
2, rosickyite azoles and fluorine pyrrole bacterium acid amides are to the indoor joint toxicity measuring of root knot nematode in tobacco.
Test method: gather field tobacco root knot sample, tissue mashing, cross 200,300,500 order nematode standard screens successively, with the worm's ovum on aseptic water washing 500 mesh sieve, be mixed with ovum suspended emulsion, in 25 DEG C of insulating boxs, hatch 3-4d, collect second instar larvae and add sterile water to be mixed with finite concentration (about 500/ml) suspended emulsion for subsequent use.
Setting 5 concentration gradient process, take sterile water as blank.Get 96 porocyte culture plates, every hole adds medicament 150 microlitre of the variable concentrations prepared, add isopyknic nematode suspension again, under 25 DEG C of conditions, moisturizing is cultivated, the amount of survival of 48h microscopy nematode and dead quantity after treatment, nematode is stiff motionless for dead worm, and nematode is the worm that lives in bending wriggling state.Calculate lethality and corrected mortality.
Carry out statistical analysis with DPS statistical analysis software, calculate the LC of each medicament
50, and the co-toxicity coefficient (CTC value) of mixture is calculated according to the abundant method of Sun Yun.
Actual measurement toxicity index (ATI)=(standard agent LC
50/ reagent agent LC
50) × 100.
In theoretical toxicity index (TTI)=A medicament toxicity index × mixture A percentage composition+B medicament toxicity index × mixture in the percentage composition of B.
Co-toxicity coefficient (CTC)=[the theoretical toxicity index (TTI) of mixture actual measurement toxicity index (ATI)/mixture] × 100.
According to the NY/T11547.7-2006 insecticide combination effect criteria for classifying: co-toxicity coefficient (CTC) >=120 shows as synergistic effect; Co-toxicity coefficient (CTC)≤80 shows as antagonism; 80 < co-toxicity coefficient (CTC) < 120 show as summation action.
Table 2, rosickyite azoles and fluorine pyrrole bacterium acid amides are to the indoor joint toxicity measuring result of root knot nematode in tobacco
| Medicament title and proportioning (weight ratio) | LC 50(mg/L) | ATI | TTI | CTC |
| Rosickyite azoles | 10.76 | 100.00 | - | - |
| Fluorine pyrrole bacterium acid amides | 7.62 | 141.21 | - | - |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (30:1) | 6.25 | 172.16 | 101.33 | 169.90 |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (20:1) | 5.34 | 201.50 | 101.96 | 197.63 |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (10:1) | 4.39 | 245.10 | 103.75 | 236.24 |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (5:1) | 3.75 | 286.93 | 106.87 | 268.49 |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (1:1) | 3.67 | 293.19 | 120.61 | 243.09 |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (1:5) | 3.91 | 275.19 | 134.34 | 204.85 |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (1:10) | 4.45 | 241.80 | 137.46 | 175.91 |
| Rosickyite azoles: fluorine pyrrole bacterium acid amides (1:20) | 5.37 | 200.37 | 139.25 | 143.89 |
As can be seen from Table 2, rosickyite azoles and fluorine pyrrole bacterium acid amides composite in the scope of 30:1 ~ 1:20 (by weight) time, co-toxicity coefficient (CTC) is all greater than 120, all shows synergistic function to root knot nematode in tobacco; Rosickyite azoles and fluorine pyrrole bacterium acid amides are in the scope of 10:1 ~ 1:5 (by weight), and synergistic function is more remarkable, and co-toxicity coefficient (CTC) is all more than 200; Rosickyite azoles and fluorine pyrrole bacterium acid amides composite with 5:1 (by weight), synergistic function is the most remarkable, and co-toxicity coefficient (CTC) is 268.49.Visible, rosickyite azoles and fluorine pyrrole bacterium acid amides is composite has reasonability and feasibility.
In sum, the present invention adopt two kinds of active ingredient rosickyite azoles and fluorine pyrrole bacterium acid amides composite, obvious synergy, compared with existing unitary agent, quick-acting is good, and the lasting period is long, control nematode successful improves, and to crop safety, is worth applying in agricultural production.
Claims (4)
1. one kind contains the composition pesticide of rosickyite azoles and fluorine pyrrole bacterium acid amides, it is characterized in that: active ingredient is rosickyite azoles and fluorine pyrrole bacterium acid amides, the weight ratio of rosickyite azoles and fluorine pyrrole bacterium acid amides is 30:1 ~ 1:20, and rosickyite azoles and fluorine pyrrole bacterium acid amides gross weight percentage composition are in the composition 0.1% ~ 80%.
2. a kind of composition pesticide containing rosickyite azoles and fluorine pyrrole bacterium acid amides according to claim 1, is characterized in that: the weight ratio of described rosickyite azoles and fluorine pyrrole bacterium acid amides is 10:1 ~ 1:5.
3. a kind of composition pesticide containing rosickyite azoles and fluorine pyrrole bacterium acid amides according to claim 1, is characterized in that: the formulation of described composition pesticide is the one in microcapsule suspending agent, suspending agent, aqueous emulsion, microemulsion, water dispersible granules, wetting powder, granule and missible oil.
4. a kind of composition pesticide containing rosickyite azoles and fluorine pyrrole bacterium acid amides according to claim 1 is preventing and treating the application in crops nematode.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106342903A (en) * | 2016-08-23 | 2017-01-25 | 赖丰光 | Pesticide composition containing pesticidal plant |
| CN113287623A (en) * | 2021-05-25 | 2021-08-24 | 贵州道元生物技术有限公司 | Compound bactericide for preventing and treating tobacco root knot nematode disease |
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2015
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106342903A (en) * | 2016-08-23 | 2017-01-25 | 赖丰光 | Pesticide composition containing pesticidal plant |
| CN113287623A (en) * | 2021-05-25 | 2021-08-24 | 贵州道元生物技术有限公司 | Compound bactericide for preventing and treating tobacco root knot nematode disease |
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Application publication date: 20151125 |