CN106942280A - Nematicidal composition containing tioxazafen and Paecilomyces lilacinus - Google Patents

Nematicidal composition containing tioxazafen and Paecilomyces lilacinus Download PDF

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Publication number
CN106942280A
CN106942280A CN201710178903.2A CN201710178903A CN106942280A CN 106942280 A CN106942280 A CN 106942280A CN 201710178903 A CN201710178903 A CN 201710178903A CN 106942280 A CN106942280 A CN 106942280A
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China
Prior art keywords
tioxazafen
paecilomyces lilacinus
nematicidal composition
root
active component
Prior art date
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Pending
Application number
CN201710178903.2A
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Chinese (zh)
Inventor
耿鹏
王劲
叶宇平
杨建江
刘映
冯宝仪
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Foshan Agricultural Co Ltd
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Foshan Agricultural Co Ltd
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Priority to CN201710178903.2A priority Critical patent/CN106942280A/en
Publication of CN106942280A publication Critical patent/CN106942280A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/82Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides a kind of using tioxazafen and Paecilomyces lilacinus as the nematicidal composition of principle active component, the proportioning of active component is 100 in the nematicidal composition:1‑1:6, obvious synergy is shown between active component in said ratio, active ingredient usage amount can be reduced, prevention effect is improved.The nematicidal composition of the present invention can be widely used in preventing and treating crop root knot nematode disease caused by a variety of pathogenic nematodes with any one in missible oil, granule, aqueous emulsion, suspending agent, microcapsule suspending agent, suspension seed-coating agent etc..

Description

Nematicidal composition containing tioxazafen and Paecilomyces lilacinus
Technical field
The invention belongs to composition pesticide field, and in particular to be that one kind includes tioxazafen and Paecilomyces lilacinus Nematicidal composition and its application.
Background technology
The root of the various vegetables of root-knot nematode main harm, shows as lateral root and fibrous root more normally increases, and in young root The spherical or conical white root nodule differed in size is formed on fibrous root, what is had is in beads shape.Killed strain overground part growth is short and small, slow Slowly, leaf color is abnormal, as a result few, yields poorly, or even cause plant to do sth. in advance death.Being currently known the nematode of danger to vegetables mainly has height Bend root-knot nematode, peanut root-knot nematode, M hapla and Meloidogyne incognita.Nematode host range is extensive, melon of often causing harm The various vegetables such as class, solanaceous vegetables, beans and radish, lettuce, Chinese cabbage, moreover it is possible to propagate some fungies and bacterial disease.
Nematicide tioxazafen, chemical name is 3- phenyl -5-(The base of thiophene -2)- 1,2,4- oxadiazoles, are by Meng The oxadiazole class nematicide of Shan Dou companies report, is mainly used in pest control and the nematode processing of soil, its molecular structure It need not use that smell is big, with serious pollution raw material without N, P element, in building-up process, the effect of clean manufacturing can be reached, not Come, nematicide tioxazafen is expected to substitute nematicidal products of the nematicides such as fosthiazate as main flow.
Paecilomyces lilacinus, is effective bacterial parasite of Meloidogyne incognita and white cyst roundworm ovum, to Meloidogyne incognita Percentage of egg parasitism be up to 60-70%.There is prevention effect to a variety of nematodes, be the preventing and treating most promising biological prevention and control agent of root-knot nematode. By this bacterium commercialization, this fungi preparation is sold with BIOCON in Philippine Asia technique center.At home, Hua Yuanfeng agricultures are biological Science and Technology Ltd. releases 100g/ bottles of bottled preparations with a 10000000000/gram spore preparation living, has listed.Paecilomyces lilacinus secondary generation Thank to the physiological function of thing:Root system of plant, which applies bacterium, can not only substantially suppress nematode infection, and can promote the life of vegetable nutritorium It is long, while the sprouting and growth to seed also have facilitation;Abundant chitinase can be produced, chitinase has to chitin Degradation, it can promote the hatching of line eggs, improve parasitic rate of the paecilomycerol to nematode;Difficultly-soluble phosphates can be promoted Dissolving, laboratory research is confirmed:The solubilizing effect of Paecilomyces varioti reaches 30%, and nematodes Antagonistic Fungi reaches 20-40%.Study simultaneously It is also shown that Paecilomyces varioti can also promote many chemical polymerization things(Such as agricultural chemicals, leather-making waste water)Decomposition, this prove Paecilomyces lilacinus With certain environmental protection effect.
The biological control of plant nematode diseases has boundless market prospects in soil, for a long time by plant The biological control research of parasitic nematode, which obtains some, has the bacterial strain of certain biological control, but in actual applications, due to life The preventing efficiency of thing preventing and treating is extremely limited, thus the composition that some can compound that requires study, when especially compounding together Composition with synergistic function seemed and be extremely necessary.
In this context, present inventor determines and field control effectiveness test screening by substantial amounts of toxicity, hair There is certain synergistic function after now compounding both tioxazafen and Paecilomyces lilacinus, complete after further research The present invention.In addition, at present it is not yet found that closing the relevant report for compounding tioxazafen and Paecilomyces lilacinus.
The content of the invention
Preferably prevent in view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of preventing and treating to root-knot nematode and have The Synergistic composition of effect is controlled, the composition active component mainly includes Paecilomyces lilacinus and tioxazafen, wherein, Paecilomyces lilacinus and tioxazafen weight ratio are 100:1-1:6, wherein, the amount containing viable bacteria of Paecilomyces lilacinus is 1-100 hundred million CFU/g, preferably 500,000,000 CUF/g.
Further, the weight ratio of the Paecilomyces lilacinus and tioxazafen is 100:1-5:3.
Further, the weight percentage of composition shared by nematicidal composition active component of the invention is 0.5- 80%, preferably 2-50%, more preferably 5-30%, remaining is auxiliary agent and carrier conventional in Pesticide formulation.
Further, nematicidal composition of the invention is prepared into by the conventional preparation method of those skilled in the art and appointed Anticipate a kind of preparation, appoint including but not limited in missible oil, granule, aqueous emulsion, suspending agent, microcapsule suspending agent, suspension seed-coating agent etc. Meaning is a kind of.
Another object of the present invention is to provide the purposes of the nematicidal composition, the nematicidal composition is especially fitted For preventing and treating cucumber, corn, tomato, soybean, peanut, wheat, the root-knot nematode of seeding corn and other crops, SCN and golden nematode The colonization entozoa of category.
Further, the nematicidal composition can also include other agricultural active composition, including but not limited to other The nematicide of species, Insecticides (tech) & Herbicides (tech), bactericide, herbicide, synergist, biostimulant, plant growth regulator.
Further, the nematicidal composition can be applied to soil or plant, for example, be administered to soil before planting Earth, soil can be also administered to after planting.
Further, nematicidal composition can be applied to soil by the nematicidal composition using drip irrigation system.
Compared with prior art, nematicidal composition of the invention has the beneficial effect that:
1st, active component tioxazafen and Paecilomyces lilacinus component compounding are reasonable in nematicidal composition of the invention, Liang Zhefu There is synergistic function with the preventing and treating in a specific range to root-knot nematode, both compounding uses, which advantageously reduce agricultural chemicals, to be made Consumption, improves prevention effect;
2nd, nematicide action site of the invention is different, can delay the drug-fast generation of nematode, be conducive to extending chemical agriculture The service life of medicine, so as to reduce drug cost;
3rd, nematicidal composition of the invention has the advantages that technique is easy to control, product quality is stable, can continuously produce, be adapted to In industrialization.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, present invention specific examples below Illustrate, but the present invention is limited to absolutely not these examples.As described below is only the present invention preferably embodiment, is used only for explaining The present invention, but can not be therefore understands that being the limitation to the scope of the claims of the present invention.It should be pointed out that it is all the present invention spirit and Any modification, equivalent or improvement for being made within principle etc., should be included within the scope of the present invention, therefore, The protection domain of patent of the present invention should be determined by the appended claims.
First, example of formulations
The present invention example of formulations in, Paecilomyces lilacinus for 500,000,000 live spore/g granules.
1st, embodiment 1:Granule containing Paecilomyces lilacinus and tioxazafen, is constituted as follows:
Tioxazafen 60%
Paecilomyces lilacinus 3%
Cornstarch 6%
White granulated sugar 3%
White carbon 5%
Xanthans 0.6%
Kaolin surplus
The preparation method of the granule is:By it is above-mentioned match somebody with somebody direction mixing kettle in add kaolin, Paecilomyces lilacinus, Tioxazafen, white granulated sugar, white carbon, cornstarch and xanthans mixing.Enter promoting the circulation of qi into airslide disintegrating mill after well mixed Stream is crushed.Mixed powder after air-flow crushing is collected into kneader, is mediated after adding the water of mixed powder total amount 13%, is mediated and complete Enter Squeezinggranulator afterwards to granulate, enter back into fluidized bed dryer drying.Eventually pass pelletizing machine whole grain and be made of the present invention Contain Paecilomyces lilacinus and Tioxazafen granules.
2nd, embodiment 2:Wettable powder containing Paecilomyces lilacinus and tioxazafen, is constituted as follows:
Tioxazafen 40%
Paecilomyces lilacinus 4%
Lauryl sodium sulfate 3%
Sodium lignin sulfonate 5%
Meteorological white carbon 5%
White granulated sugar 2%
Kaolin surplus
The preparation method of the wettable powder is:By it is above-mentioned match somebody with somebody direction mixing kettle in add kaolin, lauryl sodium sulfate, Sodium lignin sulfonate, Paecilomyces lilacinus, Tioxazafen, gas-phase silica and white granulated sugar, enter air-flow crushing after stirring Machine air-flow crushing, makes the powder after its fineness≤45 micron, collection air-flow crushing stir i.e. obtained of the present invention containing light The wettable powder of purple Paecilomyces varioti and Tioxazafen.
3rd, embodiment 3:Water dispersible granules containing Paecilomyces lilacinus and tioxazafen, are constituted as follows:
Tioxazafen 30%
Paecilomyces lilacinus 3%
Lauryl sodium sulfate 3.5%
Sodium lignin sulfonate 6.5%
Meteorological white carbon 7%
Sodium Hydroxymethyl Stalcs 5%
White granulated sugar 2%
Arabic gum 4%
Bentonite surplus
The preparation method of the water dispersible granules is:By it is above-mentioned match somebody with somebody direction mixing kettle in add bentonite, lauryl sodium sulfate, Sodium lignin sulfonate, Paecilomyces lilacinus, Tioxazafen, gas-phase silica, Arabic gum, white carbon and sodium carboxymethyl starch, Enter Pneumatic crushing machine gaseous stream after stirring to crush, make its fineness≤45 micron, the mixed powder after air-flow crushing is collected into In kneader, mediated after adding the water of mixed powder total amount 10%, Squeezinggranulator is entered after the completion of kneading and is granulated, fluid bed is entered back into Drier is dried.Eventually pass pelletizing machine whole grain and be made of the present invention containing Paecilomyces lilacinus and Tioxazafen water-dispersible grains Agent.
4th, comparative example 1:30%dioxazafen water dispersible granules(Preparation method be the same as Example 3)
2nd, Assays Example
Using Paecilomyces lilacinus and tioxazafen processing cucumber root-knot nematodes, including preventing and treating root-knot nematode and two kinds are used alone Active ingredient is mixed applies preventing and treating root-knot nematode, and line is killed using the Paecilomyces lilacinus and tioxazafen of different ratio of the present invention Worm composition is used to prevent and treat cucumber root-knot nematode field control effectiveness test, often handles in triplicate, per cell one repetition, using with Machine goes to rent and arranged, the m of plot area 202.It is shallow mixed in broadcasting preplanting treatment dispenser, it is random to sow.Respectively at 30 days investigation root knots after medicine Nematode population.Using diagonal sampling method, every samples 3 plants, the record that investigation disease index and incidence of disease root-knot nematode endanger Standard, raw many major general's state of an illness according to root knot and is divided into Pyatyi:
0 grade without root knot;
1 grade of root knot accounts for the 1-25% of full root system;
2 grades of root knots account for the 26-50% of full root system;
3 grades of root knots account for the 51-75% of full root system
4 grades of root knots account for the 76-100% of full root system
According to formula(1)And formula(2)Calculate the prevention effect of cucumber root-knot nematode:
The value of preventive effect refers to that the percentage for killing nematode is represented, the synergy situation of two kinds of medicaments, experiment are calculated according to colby formula As a result it is as shown in table 1.
Raw test result shows, 500,000,000 work spore/g Paecilomyces lilacinus granules are compounded into rear defence with 30%tioxazafen Root-knot nematode effect is controlled preferably, after both compound, 100:1-1:Certain synergistic function is shown in the range of 6. Wherein, when both compound proportions are 100:When 1, the consumption of chemical pesticide can be reduced to 0.05Kg preparations, while preventive effect reaches To more than 90%, show that the composition of the present invention can reduce chemical pesticide usage amount after compounding, improve prevention effect.

Claims (6)

1. a kind of nematicidal composition, it is characterised in that active component includes Paecilomyces lilacinus and tioxazafen.
2. nematicidal composition according to claim 1, it is characterised in that the Paecilomyces lilacinus and tioxazafen Weight ratio is 100:1-1:6.
3. nematicidal composition according to claim 1 or 2, it is characterised in that the Paecilomyces lilacinus and Tioxazafen weight is 100 than being most preferably:1.
4. nematicidal composition according to claim 2, it is characterised in that the viable bacteria amount of the Paecilomyces lilacinus is 1- 10000000000 CFU/g.
5. the nematicidal composition according to any one of claim 1-3 claim, it is characterised in that described to kill nematode Composition can also include other agricultural active composition.
6. the nematicidal composition according to claim 1-6 is used for the purposes for preventing and treating crops root-knot nematodes.
CN201710178903.2A 2017-03-23 2017-03-23 Nematicidal composition containing tioxazafen and Paecilomyces lilacinus Pending CN106942280A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108294050A (en) * 2018-03-16 2018-07-20 佛山市普尔玛农化有限公司 Including Paecilomyces lilacinus kills line composition
EP3818829A3 (en) * 2019-10-16 2021-07-21 Monsanto Technology LLC Granular nematicide compositions

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102405939A (en) * 2011-09-27 2012-04-11 德强生物股份有限公司 Nematicidal combination comprising paecilomyces lilacinus and carbosulfan
CN102422844A (en) * 2011-09-27 2012-04-25 德强生物股份有限公司 Paecilomyces lilacinus-containing composition for preventing and controlling nematodes
CN106035386A (en) * 2016-08-01 2016-10-26 佛山市盈辉作物科学有限公司 Nematicidal composition containing paecilomyces lilacinus and fosthiazate
CN106259469A (en) * 2016-08-18 2017-01-04 田文华 A kind of fluorine-containing thiophene worm sulfone and the nematicidal composition of Paecilomyces lilacinus
CN106508969A (en) * 2016-11-07 2017-03-22 佛山市盈辉作物科学有限公司 Fluazaindolizine-Paecilomyces lilacinus-containing nematicide composition

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102405939A (en) * 2011-09-27 2012-04-11 德强生物股份有限公司 Nematicidal combination comprising paecilomyces lilacinus and carbosulfan
CN102422844A (en) * 2011-09-27 2012-04-25 德强生物股份有限公司 Paecilomyces lilacinus-containing composition for preventing and controlling nematodes
CN106035386A (en) * 2016-08-01 2016-10-26 佛山市盈辉作物科学有限公司 Nematicidal composition containing paecilomyces lilacinus and fosthiazate
CN106259469A (en) * 2016-08-18 2017-01-04 田文华 A kind of fluorine-containing thiophene worm sulfone and the nematicidal composition of Paecilomyces lilacinus
CN106508969A (en) * 2016-11-07 2017-03-22 佛山市盈辉作物科学有限公司 Fluazaindolizine-Paecilomyces lilacinus-containing nematicide composition

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108294050A (en) * 2018-03-16 2018-07-20 佛山市普尔玛农化有限公司 Including Paecilomyces lilacinus kills line composition
EP3818829A3 (en) * 2019-10-16 2021-07-21 Monsanto Technology LLC Granular nematicide compositions

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Application publication date: 20170714