CN101227822A - Agent for controlling diseases occurring during rice seedling raising period - Google Patents
Agent for controlling diseases occurring during rice seedling raising period Download PDFInfo
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- CN101227822A CN101227822A CNA2006800267054A CN200680026705A CN101227822A CN 101227822 A CN101227822 A CN 101227822A CN A2006800267054 A CNA2006800267054 A CN A2006800267054A CN 200680026705 A CN200680026705 A CN 200680026705A CN 101227822 A CN101227822 A CN 101227822A
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- seedling
- rice
- disease
- bacterium
- control agent
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Mycology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
- Catching Or Destruction (AREA)
Abstract
The present invention addresses the problem of providing a microbial pesticide which is safe and has excellent control effects on many diseases occurring during the rice seedling raising period and which has little environmental impact. According to the present invention, there is provided an agent for controlling a disease occurring at a rice seedling growing stage, which agent contains a filamentous fungus belonging to the genus Talaromyces having an antagonistic action against a pathogenic bacterium causing a disease at the rice seedling growing stage.
Description
Technical field
The method of preventing and treating of disease that the present invention relates to the control agent of the disease that the seedling cultivation of rice phase takes place and use the seedling cultivation of rice phase generation of this control agent.
Background technology
For the cultivation of paddy rice, the healthy and uniform seedling of cultivation is one of most important operation, but at nursery stage, promptly by germinateing to the various diseases of easy infection of seedling phase, normally prevents and treats completely by chemical agent.
As the employed chemosynthesis medicament of the seedling cultivation of rice phase disease control of carrying out in the past, for example seed transmission venereal disease evil-bakanae disease of rice, rice blast, the helminthosporium leaf spot that infects for mold fungi used the DMI agent of fluorine bacterium azoles etc. usually, for the then widely-used quinoline acid etc. of the seed transmission venereal disease evil-paddy rice Ying rot of bacterial infection, bacillary seedling blight, brown streak.In addition, then prevent and treat for the disease-paddy rice seedling blight of soil infection by the medicament that contains the different azoles of hydroxyl, benomyl etc.
But, pathogen low to above-mentioned chemosynthesis medicament sensitivity or that have resistance has appearred.In addition, for the worry for the influence of foodsafety or environment, in crop genetics, people wish to reduce the usage amount or the access times of chemosynthesis medicament.And along with the raising of requirements such as foodsafety, people wish to develop and can reduce the usage amount of chemosynthesis medicament or the technology/commodity of access times in the crop seedling phase.Above-mentioned technology has the safe chemicals of uses such as can be used as natural goods, food additives specifically or utilizes biological agricultural chemicals etc.
As utilizing biological disease control technology, known have by microorganism formulation prevent and treat.For example put down in writing the rice disease control microorganism formulation that contains the Fusarium bacterium in the patent documentation 1, put down in writing the paddy rice seedling diseases evil control microorganism formulation that contains the pseudomonas bacterium in the patent documentation 2.
In addition, (Talaromyces avus) can be used as the cauline leaf disease control agent of strawberry anthracnose and powdery mildew of strawberry as the microbial bactericide " Biotrust " (Idemitsu Kosen Co., Ltd.) of active ingredient to use hyphomycetic a kind of yellow ankle joint bacterium.But, do not have report to confirm that ankle joint bacterium also has disease-controlling effect for other crop at present as yet.
Patent documentation 1: Japanese kokai publication hei 11-89562 communique
Patent documentation 2: TOHKEMY 2002-17343 communique
Summary of the invention
The present invention is derived from above-mentioned viewpoint, and its purpose is to provide control efficiency excellence, safety and the microbial pesticide little to environmental influence of a lot of diseases that the seedling cultivation of rice phase is taken place.
The inventor etc. have carried out deep research for solving above-mentioned problem, and found that: a lot of diseases that the mold fungi of Talaromyces was taken place for the seedling cultivation of rice phase have control efficiency, have finished the present invention according to this understanding.
That is, main contents of the present invention are as follows.
(1) the control agent of the disease of seedling cultivation of rice phase generation, this control agent contain the mold fungi that belongs to Talaromyces (Talaromyces) that the pathogen that causes disease in the seedling cultivation of rice phase is had antagonism.
(2) the control agent of the disease of the seedling cultivation of rice phase of (1) generation, wherein, the pathogen that causes disease in the seedling cultivation of rice phase is rice paddy seed contagiosity cause of disease bacterium or soil infection pathogen.
(3) the control agent of the disease of the seedling cultivation of rice phase of (2) generation, wherein, the pathogen in seedling cultivation of rice phase initiation disease is that fusarium moniliforme-rice is disliked seedling red mould (Gibberella fujikuroi), rice blast fungus-piricularia oryzae (Pyricularia oryzae), Hemintho-sporum leaf spot of rice plants bacterium-palace portion cochliobolus (Cochliobolus miyabeanus), bacillary paddy rice Ying rot bacterium-clever shell pseudomonad (Pseudomonas glumae), bacillary paddy rice seedling blight bacterium-Pseudomonas plantarii (Pseudomonas plantarii), paddy rice brown streak bacterium-Pseudomonas avenae (Pseudomonas avenae), Fusarium (Fusarium) bacterium as paddy rice seedling blight bacterium, pythium (Pythium) bacterium, rhizopus (Rhhizopus) bacterium, at least a in trichoderma (Trichoderma) bacterium.
(4) each disease control agent in (1)～(3), wherein, the mold fungi that belongs to Talaromyces be yellow ankle joint bacterium (Talaromyces, avus).
(5) the disease control agent of (4), wherein, the mold fungi that belongs to Talaromyces is yellow ankle joint bacterium Y-9401 (Talaromyces flavus Y-9401) bacterial strain (FERM BP-10642).
(6) method of preventing and treating of the disease that takes place of seedling cultivation of rice phase is characterized in that: this method comprises the step of seed, the seedling of paddy rice, grow seedlings soil or raise seedling culture medium being handled by each disease control agent in (1)～(5).
The best mode that carries out an invention
Below describe the present invention in detail.
(disease control agent of the present invention)
Disease control agent of the present invention is the control agent of the disease of seedling cultivation of rice phase generation.Here, " seedling " is meant the plantling body of paddy rice before field planting." grow seedlings " and be meant that cultivation seedling, " nursery stage " are meant plantation rice shoot culture time before.The shoot root of nursery stage is represented the growth period of seedling according to the number of the leaf that seedling had, as a leaf phase, two leaf stage, tri-leaf period etc.In the growth period of seedling, the seedling of nursery stage for example is meant the suitable phase of seedling replanting, that is, and and to the seedling of four leaves about the phase that comprises imperfect leaf.
Disease control agent of the present invention contain belong to Talaromyces mold fungi as active ingredient.(1) belongs to the mold fungi of Talaromyces
The mold fungi that belongs to Talaromyces of using among the present invention is not particularly limited so long as the bacterium that the pathogen that causes disease in the seedling cultivation of rice phase is had an antagonism gets final product.Here, " antagonism " is to instigate effect or the effect that suppresses its propagation, the i.e. antibacterial action that reduces as cell number object, cause the pathogen of disease in the seedling cultivation of rice phase.The bacterium that the pathogen that causes disease in the seedling cultivation of rice phase is had an antagonism preferably belongs to the yellow ankle joint bacterium (Talaromyces of Talaromyces, flavus) bacterium of Zhonging, wherein further preferred yellow ankle joint bacterium Y-9401 (Talaromyces, flavus Y-9401) bacterial strain.As long as having above-mentioned antagonism, also can use the mutant strain of Y-9401 bacterial strain.In the disease control agent of the present invention, can with in the mentioned microorganism a kind of separately or in the scope that does not influence disease-controlling effect, be used in combination of two or more.
Yellow ankle joint bacterium Y-9401 (Talaromyces flavus Y-9401) bacterial strain is deposited in Japanese trading industry on September 2nd, 1996 and economizes Industrial Technology Institute life engineering Industrial Technology Research Institute special permission microorganism sustenance center (existing Independent Administrative Leged Industrial Technology Complex Inst speciallys permit biological sustenance center (IPOD) (east, ripple city 1-1-1 central authorities the 6th are built in the Ibaraki, Japan)), and preserving number is FERMP-15816.Transfer international preservation on July 18th, 2006, preserving number is FERMBP-10642.
For example can followingly confirm for the antagonism that causes the pathogen of disease in the seedling cultivation of rice phase: on same culture plate, will be arranged side by side for the pathogen of examination bacterium and seedling cultivation of rice phase initiation disease, face-off was cultivated 3～14 days under 15～35 ℃, observed then for trying the inhibition situation that bacterium grows the growth of pathogenic bacteria that causes disease in the seedling cultivation of rice phase.
The mold fungi that belongs to Talaromyces of using among the present invention can be according to the method cultivation same with common mold fungi.For example, can make the mold fungi propagation that belongs to Talaromyces by liquid culture method such as the reciprocating type liquid culture of cultivating in the liquid medium within or jar fermentor cultivation or the plate method of in solid culture medium, cultivating.Produce the hyphomycetic spore that belongs to Talaromyces well for yield, preferably adopt plate method.The hyphomycetic condition of culture that belongs to Talaromyces preferably by methods such as ventilation, stirring, vibrations, under aerobic condition, carry out preferred 20～40 ℃ of cultivation temperature.Between culture period preferred 3～60 days, preferred especially 3～20 days.Consider that from the keeping quality angle of disease control agent product the hyphomycetic thalline that belongs to Talaromyces that uses among the present invention is preferably spore.Therefore, in order to make the mold fungi that belongs to Talaromyces form spore, can be as required at the pH of the composition of cultivating of regulating medium latter stage, medium, cultivation temperature, cultivation humidity, condition of culture such as oxygen concentration when cultivating, make it be fit to the condition of this sporulation.
Employed liquid culture based component can use potato glucose medium or sabouraud culture medium etc. when the mold fungi that belongs to Talaromyces was cultivated.When carrying out solid culture, can be as required cereals such as rice, wheat, corn, soybean, chaff, dregs of beans etc. are from the solid constituent of cereal, or contain on the solid carriers such as clay mineral of nutrient source and use the medium that contains carbohydrate or nitrogenous source etc.
Containing the hyphomycetic culture that belongs to Talaromyces can directly use, also can be as required that culture is broken or chop up use, is further used by the main spore that reclaims in this culture by sieve etc.In addition, the separating thallus from culture by water or wet goods liquid can directly use or use concentrate.
Disease control agent of the present invention can be to belong to the form of the mold fungi of Talaromyces with common colony-forming units, will contain 1 * 10
6～1 * 10
12Cfu/g, preferred 1 * 10
7～1 * 10
11The culture of cfu/g or the crushed material of culture are coupled in the various preparations that adapt with purposes or using method and use.
In the disease control agent of the present invention, the content that contains the crushed material of the hyphomycetic culture that belongs to Talaromyces or culture is preferably 0.1～99.9% quality, more preferably 1.0～50.0% quality.
(2) any composition
Only otherwise anti-evil effect of the present invention, remove in the disease control agent of the present invention the above-mentioned mold fungi that belongs to Talaromyces, can also add any composition use.Above-mentioned any composition can be that purpose contains with preparationization, stay in gradeization etc.
Any composition that uses in the disease control agent of the present invention for example can use a kind of of following compositions or be used in combination of two or more.
For example as incremental agent, solid carrier has mineral matter micropowders such as kaolin, pyrophyllite soil, bentonite, montmorillonite, diatomite, synthetic oxidizing aqueous silicon, Emathlite, talcum powder class, clay, pottery, quartz, sericite, vermiculite, perlite, large space between muscles stone, ア Application ス ラ stone, lime stone, coal stone, zeolite; Inorganic compounds such as salt, carbonate, sulphate, nitrate, urea; Organic matter micropowders such as rice husk, chaff, crab shell, shrimp shell, krill micropowder, rice are poor, wheat flour, corncob, peanut shell, bone meal, fish meal, dregs of rice powder, wood powder, charcoal, smoked charcoal, bark charcoal, the smoked charcoal of rice husk, ash, peat, attapulgite, the dry ight soil of livestock, active carbon, oil meal, starch and hydrolysate thereof; Solubility incremental agents such as D-sorbierite, lactose, maltose, aminoglucose, oligosaccharides class etc.Liquid-carrier can make water, vegetable oil, animal oil, mineral oil, synthesizing water-solubility macromolecule etc.
Can also be as required, with thickening, adhere to, dispersion etc. is purpose, contains natural polysaecharides such as casein as adjuvant, gelatin, gum Arabic, alginic acid, cellulose family, carboxymethyl cellulose, xanthans, chitin class, shitosan class etc.; Polyvinyl alcohol; Polyacrylic; Bentonite etc.
Can also be purpose to prevent freezing grade, contain dihydroxylic alcohols such as ethylene glycol, propane diols etc. as required.
For stably dispersing, prevent gathering, emulsification etc., can contain surfactants such as anionic, cationic, amphoteric as required.
(preparation method of disease control agent of the present invention)
Of the present inventionly contain the hyphomycetic disease control agent that belongs to Talaromyces and can make the form of easy practical application.That is to say, can as required, make preparations such as hydrating agents, pulvis, granule, emulsion, suspending agent, smears according to the preparation method of common preparation with various any compositions.
For example, hydrating agents, pulvis can mix in above-mentioned solid carrier as required or pulverize the one-tenth that mixes above-mentioned surfactant or make stay in grade and assign to prepare.
For example granule can mix in above-mentioned solid carrier as required or pulverize the composition that mixes above-mentioned surfactant or make stay in grade, prepares again.
Example emulsion can be as required,, in vegetable oil, animal oil, the aqueous carrier of mineral wet goods, mix or pulverize that to mix with emulsification, dispersion etc. be the preparation of assigning to of the above-mentioned surfactant of purpose or the one-tenth that makes stay in grade.
For example suspending agent can be in water with thickening etc. be purpose mix or pulverize mix above-mentioned auxiliary agent, with prevent freezing etc. be purpose mix or pulverize mix above-mentioned dihydroxylic alcohols etc., be that purpose is mixed or pulverized and mixes above-mentioned surfactant to disperse etc., and mix or pulverize the one-tenth that mixing makes stay in grade and assign to prepare.
For example the smears can be to add auxiliary agent in water or wet goods liquid-carrier, mixes, and makes colloidal sol shape or gel and prepares.
(application process of disease control agent of the present invention)
Disease control agent of the present invention can be brought into play the preventive and therapeutic effect of the disease of seedling cultivation of rice phase generation effectively by mold fungi, bacterium, microbial medium virus etc.Disease control agent of the present invention for example can prevent and treat effectively for the disease that is caused by rice paddy seed contagiosity germ or soil infection germ.More particularly, disease control agent of the present invention is brought into play especially effectively by fusarium moniliforme-rice and is disliked seedling red mould (Gibberella fujikuroi), rice blast fungus-piricularia oryzae (Pyricularia oryzae), Hemintho-sporum leaf spot of rice plants bacterium-palace portion cochliobolus (Cochliobolusmiyabeanus), bacillary paddy rice Ying rot bacterium-clever shell pseudomonad (Pseudomonasglumae), bacillary paddy rice seedling blight bacterium-Pseudomonas plantarii (Pseudomonasplantarii), paddy rice brown streak bacterium-Pseudomonas avenae (Pseudomonas avenae), Fusarium (Fusarium) bacterium as paddy rice seedling blight bacterium, pythium (Pythium) bacterium, rhizopus (Rhizopus) bacterium, the preventive and therapeutic effect of the disease that trichoderma (Trichoderma) bacterium causes.Disease control agent of the present invention is to one or more effects in the above-mentioned pathogen.Above-mentionedly there are the seedling cultivation of rice phase of being not only, the pathogen that also causes disease period beyond nursery stage in causing the pathogen of disease in the seedling cultivation of rice phase, here, " the seedling cultivation of rice phase is caused the pathogen of disease " is meant the pathogen that causes disease at least in the seedling cultivation of rice phase, also comprises for pathogen that causes disease period beyond the seedling cultivation of rice phase and the pathogen that does not cause disease period beyond the seedling cultivation of rice phase.
In order to prevent and treat above-mentioned various disease, of the present invention containing in seed, the seedling that the hyphomycetic disease control agent that belongs to Talaromyces is applied to paddy rice, grow seedlings soil or the raise seedling culture medium, its method can suitably be selected according to types of service such as formulation or disease.Said method for example has that seed immersion treatment, seed pelleting are handled, the seed coating is handled, seed spray (comprising spraying) handle, soil spray (comprising spraying) handle, and method such as earth mixture is used, soil-perfusion is used, seedling growing box is poured into and used, the plant root is used, overground part spray liquid, overground part solid are spread fertilizer over the fields.
To rice application disease control agent the time, mixing such as bactericidal agent, insecticide, nematocide, pediculicide, weed killer herbicide, plant growth regulator, fertilizer, soil improvement material can be used, perhaps do not mix and use alternately, perhaps use simultaneously.
The amount of application that contains the hyphomycetic disease control agent that belongs to Talaromyces of the present invention is according to disease species etc. and different, cannot treat different things as the same, for example during the seed immersion treatment, as the seed soak, be that 10～1000 times (quality) used with the preparation dilution preferably, this cell concentration is generally in every 1ml soak 1 * 10
3～1 * 10
10Cfu, preferred 1 * 10
4～1 * 10
9Cfu.
When seed pelleting is handled, preferably use the preparation of 1～20% quality of seed quality, this cell concentration is that every 1g seed quality is generally 1 * 10
3～1 * 10
10Cfu, preferred 1 * 10
4～1 * 10
9Cfu.
When soil spreads fertilizer over the fields, if to the seedling growing box that is used as sprigging usually (area 1800cm for example
2About) what spray is liquid, then preferably using 50～1000ml, this cell concentration is that every 1ml spraying liquid is generally 1 * 10
3～1 * 10
10Cfu, preferred 1 * 10
4～1 * 10
10Cfu.
When using, preferably to the seedling growing box that is used as sprigging usually (area 1800cm for example with earth mixture
2About) use 0.1～100g, this cell concentration is that 1ml soil uses 1 * 10
2～1 * 10
9Cfu, preferred 1 * 10
3～1 * 10
8Cfu.
When seedling growing box perfusion is used, if to usually as the seedling growing box of sprigging (area 1800cm for example
2About) what spray is liquid, then preferably using 50～1000ml, this cell concentration is that every 1ml spraying liquid is generally 1 * 10
3～1 * 10
9Cfu, preferred 1 * 10
4～1 * 10
8Cfu.
Frequency of administration to disease control agent of the present invention is not particularly limited, and for example the pathogen that causes disease in the seedling cultivation of rice phase is used with 1～5 time frequency in the seedling cultivation of rice phase, then can suppress seedling cultivation of rice phase generation disease usually.
Before using disease control agent of the present invention and afterwards, can cultivate seedling and the paddy rice of paddy rice according to conventional method.
Embodiment
Below provide embodiment, further describe the present invention, the present invention is not subjected to the qualification of these embodiment.
＜preparation example 1 〉
(preparation of spore)
In medium, use chaff, to the kind bacterium that wherein inoculates yellow ankle joint bacterium Y-9401 (Talaromycesflavus Y-9401) (FERM BP-10642) bacterial strain, 30 ℃ of following solid culture 10 days.With the culture drying, this dried culture is sieved after cultivation finishes, remove the chaff residue, obtain containing the powder (4 * 10 of yellow ankle joint bacterium Y-9401 spore
9Cfu/g).
＜formulation example 1 〉
(preparation of preparation)
Use the powder that contains yellow ankle joint bacterium Y-9401 spore that obtains in the above-mentioned preparation example 1, making this powder that contains spore is that 10% quality, surfactant SORPOL 5082 (the chemical industry preparation of eastern nation) are that 5% quality, incremental agent clay mineral (K clay: win Guanshan Mountain mining industry manufacturing) are that 45% quality and aminoglucose (burning the preparation of Tianjin aquatic chemistry company) are 40% quality, in this ratio raw material is mixed, use ball mill pulverizer to mix and pulverizing, obtain the preparation (4 * 10 of formulation example 1
8Cfu/g).
＜embodiment 1 〉
(bakanae disease of rice control test)
(1) preparation of infection seed
In paddy field, from the paddy rice of multiple bakanae disease of rice, reclaim paddy rice (kind: seed あ I こ ま Chi).This seed infects seed as Fusarium moniliforme Sheld.
(2) chemicals treatment
To be 200 times (quality) by the preparation dilute with water of above-mentioned formulation example 1 preparation, and in this solution of 15ml, add the 5g Fusarium moniliforme Sheld and infect seed, and in dark, 30 ℃ climatic cabinate, soak and handled in 48 hours, as fungicide-treated seed.In addition, Fusarium moniliforme Sheld is infected seed and in water, carry out immersion treatment, (seed is untreated) in contrast equally.
(3) cultivation of test plant
After the above-mentioned immersion treatment, Fusarium moniliforme Sheld is infected planting seed in having filled the 100cm of seedling cultivation of rice with compost
2Plastics system Culture basin in.The zone of the seed that is untreated as the chemicals treatment district, will have been sowed as the district of being untreated in the zone of having sowed fungicide-treated seed.The chemicals treatment district and the district of being untreated respectively prepare three districts.After covering with compost with above-mentioned seedling cultivation of rice, method is carried out cultivation management according to the rules in glass greenhouse.
(4) control efficiency investigation
After sowing for two weeks, will adopt the mean value of the morbidity seedling number in three districts in each treatment region, calculate morbidity seedling rate according to following (formula 1) by the seedling of the extreme excessive growth symptom of the expression that bakanae disease causes as the morbidity seedling.According to following (formula 2), calculate the control value in chemicals treatment district by the morbidity seedling rate of this calculating, as control efficiency.
Morbidity seedling rate (%)=morbidity seedling number/all investigate seedling number * 100 (formula 1)
Control value=[(district's morbidity seedling rate that is untreated-chemicals treatment district morbidity seedling rate)/district's morbidity seedling rate is untreated] * 100 (formulas 2)
(5) result
Investigation result is as shown in table 1.As shown in Table 1, disease control agent of the present invention has shown remarkable high control efficiency to the bakanae disease of paddy rice.
Table 1
| Treatment region | Extension rate | Bakanae disease morbidity seedling rate | The control value |
| The district of being untreated | - | 55.7％ | - |
| Formulation example 1 treatment region | 200 | 3.0％ | 94.7 |
＜embodiment 2 〉
(rice blast control test)
(1) preparation of infection seed
In paddy field, from the paddy rice of multiple rice blast, reclaim paddy rice (kind: seed あ I こ ま Chi).This seed infects seed as Pyricularia oryzae.
(2) chemicals treatment
To be 200 times (quality) by the preparation dilute with water of above-mentioned formulation example 1 preparation, and in this solution of 15ml, add the 4g Pyricularia oryzae and infect seed, and in dark, 30 ℃ climatic cabinate, soak and handled in 48 hours, as fungicide-treated seed.In addition, Pyricularia oryzae is infected seed and in water, carry out immersion treatment, (seed is untreated) in contrast equally.
(3) cultivation of test plant
After the above-mentioned immersion treatment, Pyricularia oryzae is infected planting seed in having filled the 100cm of seedling cultivation of rice with compost
2Plastics system Culture basin in.The zone of the seed that is untreated as the chemicals treatment district, will have been sowed as the district of being untreated in the zone of having sowed fungicide-treated seed.The chemicals treatment district and the district of being untreated respectively prepare three districts.After covering with compost with above-mentioned seedling cultivation of rice, method is carried out cultivation management according to the rules in glass greenhouse.
(4) control efficiency investigation
After sowing for two weeks, the seedling that will express the rice blast scab on the blade face adopts the mean value of the morbidity seedling number in three districts in each treatment region as the morbidity seedling, calculates morbidity seedling rate according to above-mentioned (formula 1).According to above-mentioned (formula 2), calculate the control value in chemicals treatment district by the morbidity seedling rate of this calculating, as control efficiency.
(5) result
Investigation result is as shown in table 2.As shown in Table 2, disease control agent of the present invention has shown remarkable high control efficiency to the rice blast of paddy rice.
Table 2
| Treatment region | Extension rate | Rice blast morbidity seedling rate | The control value |
| The district of being untreated | - | 7.9％ | - |
| Formulation example 1 treatment region | 200 | 0％ | 100.0 |
＜embodiment 3 〉
(paddy bacterial Ying rot control test)
(1) preparation of infection seed
(kind: ふ さ お と め) seed joins in the solution that contains the withered bacterium germ of Ying, forcibly is inoculated in the seed under reduced pressure with paddy rice.After air-dry with this seed as the withered bacterium courses of infection of bacillary Ying seed.
(2) chemicals treatment
To be 200 times (quality) by the preparation dilute with water of above-mentioned formulation example 1 preparation, in this solution of 15ml, add the withered bacterium courses of infection of 5g Ying seed, and in dark, 30 ℃ climatic cabinate, soak and handled in 48 hours, as fungicide-treated seed.In addition, the withered bacterium courses of infection of Ying seed is carried out immersion treatment equally in water, as the seed that is untreated.
(3) cultivation of test plant
After the above-mentioned immersion treatment, with the withered bacterium courses of infection of Ying planting seed in having filled the 100cm of seedling cultivation of rice with compost
2Plastics system Culture basin in.The zone of the seed that is untreated as the chemicals treatment district, will have been sowed as the district of being untreated in the zone of having sowed fungicide-treated seed.The chemicals treatment district and the district of being untreated respectively prepare three districts.After covering with compost with above-mentioned seedling cultivation of rice, method is carried out cultivation management according to the rules in glass greenhouse.
(4) control efficiency investigation
After sowing for two weeks, with express seedling corruption, wither, the seedling of bacillary Ying's rot symptom such as albefaction is as the morbidity seedling, adopts the mean value of the morbidity seedling number in three districts in each treatment region, calculates morbidity seedling rate according to above-mentioned (formula 1).According to above-mentioned (formula 2), calculate the control value in chemicals treatment district by the morbidity seedling rate of this calculating, as control efficiency.
(5) result
Investigation result is as shown in table 3.As shown in Table 3, disease control agent of the present invention has shown remarkable high control efficiency to bacillary Ying's rot of paddy rice.
Table 3
| Treatment region | Extension rate | Bacillary Ying's rot morbidity seedling rate | The control value |
| The district of being untreated | - | 27.5％ | - |
| Formulation example 1 treatment region | 200 | 2.3％ | 91.6 |
＜embodiment 4 〉
(paddy bacterial seedling blight control test)
(1) preparation of infection seed
(kind: ふ さ お と め) seed joins in the solution that contains the upright withered bacterium germ of seedling, forcibly is inoculated in the seed under reduced pressure with paddy rice.After air-dry this seed is found withered bacterium courses of infection seed as bacillary seedling.
(2) chemicals treatment
To be 200 times (quality) by the preparation dilute with water of above-mentioned formulation example 1 preparation, 5g is joined in this solution of 15ml at 15 ℃ of upright withered bacterium courses of infection seeds of the seedling that absorbed water 5 days down, immersion treatment is 24 hours in dark, 30 ℃ climatic cabinate, as fungicide-treated seed.The upright withered bacterium courses of infection seed of seedling is carried out immersion treatment, (seed is untreated) in contrast equally in water.
(3) cultivation of test plant
After the above-mentioned immersion treatment, seedling is found withered bacterium courses of infection planting seed in having filled the 100cm of seedling cultivation of rice with compost
2Plastics system Culture basin in.The zone of the seed that is untreated as the chemicals treatment district, will have been sowed as the district of being untreated in the zone of having sowed fungicide-treated seed.The chemicals treatment district and the district of being untreated respectively prepare three districts.After covering with compost with above-mentioned seedling cultivation of rice, method is carried out cultivation management according to the rules in glass greenhouse.
(4) control efficiency investigation
After sowing for two weeks, with express seedling corruption, wither, the seedling of bacillary seedling blight symptom such as albefaction is as the morbidity seedling, adopts the mean value of the morbidity seedling number in three districts in each treatment region, calculates morbidity seedling rate according to above-mentioned (formula 1).According to above-mentioned (formula 2), calculate the control value in chemicals treatment district by the morbidity seedling rate of this calculating, as control efficiency.
(5) result
Investigation result is as shown in table 4.As shown in Table 4, disease control agent of the present invention has shown remarkable high control efficiency to the bacillary seedling blight of paddy rice.
Table 4
| Treatment region | Extension rate | Bacillary seedling blight morbidity seedling rate | The control value |
| The district of being untreated | - | 100.0％ | - |
| Formulation example 1 treatment region | 200 | 8.3％ | 91.7 |
＜embodiment 5 〉
(paddy rice brown streak control test)
(1) preparation of infection seed
(kind: ふ さ お と あ) seed joins in the solution that contains the brown streak bacterium, forcibly is inoculated in the seed under reduced pressure with paddy rice.After air-dry this seed is infected seed as the brown streak bacterium.
(2) chemicals treatment
To be 200 times (quality) by the preparation dilute with water of above-mentioned formulation example 1 preparation, 5g is infected seed 15 ℃ of brown streak bacterium that absorbed water 5 days down to join in this solution of 15ml, immersion treatment is 24 hours in dark, 30 ℃ climatic cabinate, as fungicide-treated seed.The brown streak bacterium is infected seed in water, carry out immersion treatment, (seed is untreated) in contrast equally.
(3) cultivation of test plant
After the above-mentioned immersion treatment, the brown streak bacterium is infected planting seed in having filled the 100cm of seedling cultivation of rice with compost
2Plastics system Culture basin in.The zone of the seed that is untreated as the chemicals treatment district, will have been sowed as the district of being untreated in the zone of having sowed fungicide-treated seed.The chemicals treatment district and the district of being untreated respectively prepare three districts.After covering with compost with above-mentioned seedling cultivation of rice, method is carried out cultivation management according to the rules in glass greenhouse.
(4) control efficiency investigation
After sowing for two weeks, with express seedling corruption, wither, the seedling of brown streak symptom such as brown stain is as the morbidity seedling, adopts the mean value of the morbidity seedling number in three districts in each treatment region, calculates morbidity seedling rate according to above-mentioned (formula 1).According to above-mentioned (formula 2), calculate the control value in chemicals treatment district by the morbidity seedling rate of this calculating, as control efficiency.
(5) result
Investigation result is as shown in table 5.As shown in Table 5, disease control agent of the present invention has shown remarkable high control efficiency to the brown streak of paddy rice.
Table 5
| Treatment region | Extension rate | Brown streak morbidity seedling rate | The control value |
| The district of being untreated | - | 1.9％ | - |
| Formulation example 1 treatment region | 200 | 0.3％ | 84.2 |
＜embodiment 6 〉
(the paddy rice seedling blight control test of fusarium infection)
(1) inoculation of pathogen
To evenly mix with compost with seedling cultivation of rice with the sickle-like bacteria culture that chaff and seedling cultivation of rice are cultivated with compost, obtain the paddy rice seedling blight contaminated soil of fusarium infection.
(2) chemicals treatment
100cm at the seedling blight contaminated soil that is filled with above-mentioned fusarium infection
2Plastics system Culture basin in sow paddy rice (kind: コ シ ヒ カ リ).With the preparation dilute with water of preparation in the formulation example 1 is 1000 times (quality), with gained solution to the processing (25ml) of spraying uniformly of above-mentioned soil surface, as the chemicals treatment district.In addition, prepare with the Culture basin of above-mentioned liquid medicine (district of being untreated) in contrast as water.The chemicals treatment district and the district of being untreated respectively prepare two districts.
(3) cultivation of test plant
After covering with compost with seedling cultivation of rice in the basin of having implemented above-mentioned processing, method is carried out cultivation management according to the rules in glass greenhouse.
(4) control efficiency investigation
After sowing for two weeks, with express seedling corruption, wither, the seedling of the seedling blight symptom of fusarium infection such as brown stain is as the morbidity seedling, adopts the mean value of the morbidity seedling number in two districts in each treatment region, calculates morbidity seedling rate according to above-mentioned (formula 1).According to above-mentioned (formula 2), calculate the control value in chemicals treatment district by the morbidity seedling rate of this calculating, as control efficiency.
(5) result
Investigation result is as shown in table 6.As shown in Table 6, disease control agent of the present invention has shown remarkable high control efficiency to the paddy rice seedling blight of fusarium infection.
Table 6
| Treatment region | Extension rate | The seedling blight morbidity seedling rate of fusarium infection | The control value |
| The district of being untreated | - | 20.5％ | - |
| Formulation example 1 treatment region | 1000 | 4.7％ | 77.1 |
＜embodiment 7 〉
(the paddy rice seedling blight control test that rhizopus infects)
(1) inoculation of pathogen
To evenly mix with compost with seedling cultivation of rice with the rhizopus culture that chaff and seedling cultivation of rice are cultivated with compost, obtain the paddy rice seedling blight contaminated soil that rhizopus infects.
(2) chemicals treatment
100cm at the seedling blight contaminated soil that is filled with above-mentioned rhizopus infection
2Plastics system Culture basin in sow paddy rice (kind: コ シ ヒ カ リ).With formulation example 1 dilute with water is 1000 times (quality), with gained solution to the processing (25ml) of spraying uniformly of above-mentioned soil surface, as the chemicals treatment district.In addition, prepare with the Culture basin of above-mentioned liquid medicine (district of being untreated) in contrast as water.The chemicals treatment district and the district of being untreated respectively prepare two districts.
(3) cultivation of test plant
After covering with compost with seedling cultivation of rice in the basin of having implemented above-mentioned processing, method is carried out cultivation management according to the rules in glass greenhouse.
(4) control efficiency investigation
After sowing for two weeks, with express seedling corruption, wither, the seedling of seedling blight symptom that the rhizopus of the growth inhibition of root etc. infects is as the morbidity seedling, adopts the mean value of the morbidity seedling number in two districts in each treatment region, calculates morbidity seedling rate according to above-mentioned (formula 1).According to above-mentioned (formula 2), calculate the control value in chemicals treatment district by the morbidity seedling rate of this calculating, as control efficiency.
(5) result
Investigation result is as shown in table 7.As shown in Table 7, disease control agent of the present invention has shown remarkable high control efficiency to the paddy rice seedling blight that rhizopus infects.
Table 7
| Treatment region | Extension rate | The seedling blight morbidity seedling rate that rhizopus infects | The control value |
| The district of being untreated | - | 54.1％ | - |
| Formulation example 1 treatment region | 1000 | 14.9％ | 72.5 |
＜embodiment 8 〉
(the paddy rice seedling blight control test that Trichoderma infects)
(1) inoculation of pathogen
To evenly mix with compost with seedling cultivation of rice with the rhizopus culture that chaff and seedling cultivation of rice are cultivated with compost, obtain the paddy rice seedling blight contaminated soil that Trichoderma infects.
(2) chemicals treatment
100cm at the seedling blight contaminated soil that is filled with above-mentioned Trichoderma infection
2Plastics system Culture basin in sow paddy rice (kind: コ シ ヒ カ リ).With formulation example 1 dilute with water is 1000 times (quality), with gained solution to the processing (25ml) of spraying uniformly of above-mentioned soil surface, as the chemicals treatment district.In addition, prepare with the Culture basin of above-mentioned liquid medicine (district of being untreated) in contrast as water.The chemicals treatment district and the district of being untreated respectively prepare two districts.
(3) cultivation of test plant
After covering with compost with seedling cultivation of rice in the basin of having implemented above-mentioned processing, method is carried out cultivation management according to the rules in glass greenhouse.
(4) control efficiency investigation
After sowing for two weeks, with express seedling corruption, wither, the seedling of seedling blight symptom that the Trichoderma of the growth inhibition of root etc. infects is as the morbidity seedling, adopts the mean value of the morbidity seedling number in two districts in each treatment region, calculates morbidity seedling rate according to above-mentioned (formula 1).According to above-mentioned (formula 2), calculate the control value in chemicals treatment district by the morbidity seedling rate of this calculating, as control efficiency.
(5) result
Investigation result is as shown in table 8.As shown in Table 8, disease control agent of the present invention has shown remarkable high control efficiency to the paddy rice seedling blight that Trichoderma infects.
Table 8
| Treatment region | Extension rate | The seedling blight morbidity seedling rate that Trichoderma infects | The control value |
| Come treatment region | - | 26.7％ | - |
| Formulation example 1 treatment region | 1000 | 12.6％ | 52.8 |
Industrial applicability
The present invention can provide the microorganism formulation that has the following advantages:
Bring into play high effect for the various diseases that the seedling cultivation of rice phase occurs.
Safety and little on the impact of environment.
Compare with general chemical synthetic pesticide, access times are unqualified or few.
Claims (6)
1. the control agent of the disease of seedling cultivation of rice phase generation, this control agent contain the mold fungi that belongs to Talaromyces (Talaromyces) that the pathogen that causes disease in the seedling cultivation of rice phase is had antagonism.
2. the control agent of the disease of the seedling cultivation of rice phase of claim 1 generation, wherein, the pathogen that causes disease in the seedling cultivation of rice phase is rice paddy seed contagiosity cause of disease bacterium or soil infection pathogen.
3. the control agent of the disease of the seedling cultivation of rice phase of claim 2 generation, wherein, the pathogen in seedling cultivation of rice phase initiation disease is that fusarium moniliforme-rice is disliked seedling red mould (Gibberella fujikuroi), rice blast fungus-piricularia oryzae (Pyricularia oryzae), Hemintho-sporum leaf spot of rice plants bacterium-palace portion cochliobolus (Cochliobolus miyabeanus), bacillary paddy rice Ying rot bacterium-clever shell pseudomonad (Pseudomonas glumae), bacillary paddy rice seedling blight bacterium-Pseudomonas plantarii (Pseudomonas plantarii), paddy rice brown streak bacterium-Pseudomonas avenae (Pseudomonas avenae), Fusarium (Fusarium) bacterium as paddy rice seedling blight bacterium, pythium (Pythium) bacterium, rhizopus (Rhizopus) bacterium, at least a in trichoderma (Trichoderma) bacterium.
4. each disease control agent in the claim 1～3, wherein, the mold fungi that belongs to Talaromyces is yellow ankle joint bacterium (Talaromyces flavus).
5. the disease control agent of claim 4, wherein, the mold fungi that belongs to Talaromyces is yellow ankle joint bacterium Y-9401 (Talaromyces flavus Y-9401) bacterial strain (FERM BP-10642).
6. the method for preventing and treating of the disease that takes place of seedling cultivation of rice phase is characterized in that: this method comprises the step of seed, the seedling of paddy rice, grow seedlings soil or raise seedling culture medium being handled by each disease control agent in the claim 1～5.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2005213033A JP4810151B2 (en) | 2005-07-22 | 2005-07-22 | Control agent for diseases occurring during the seedling raising season of rice |
| JP213033/2005 | 2005-07-22 |
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| CN101227822A true CN101227822A (en) | 2008-07-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNA2006800267054A Pending CN101227822A (en) | 2005-07-22 | 2006-07-21 | Agent for controlling diseases occurring during rice seedling raising period |
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|---|---|
| JP (1) | JP4810151B2 (en) |
| KR (1) | KR101280679B1 (en) |
| CN (1) | CN101227822A (en) |
| TW (1) | TW200738148A (en) |
| WO (1) | WO2007011025A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107950263A (en) * | 2011-11-04 | 2018-04-24 | 拜耳知识产权有限责任公司 | Prevent the method for transplanting rice disease |
| CN114025612A (en) * | 2019-06-27 | 2022-02-08 | 出光兴产株式会社 | Plant disease control agent and plant disease control method |
| CN114456955A (en) * | 2022-02-25 | 2022-05-10 | 中国水稻研究所 | High-efficiency inoculation method for rice bakanae disease and application thereof |
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| CN103509740B (en) * | 2013-08-13 | 2015-04-29 | 浙江大学 | Pseudomonas chlororaphis for preventing and treating crop fusarium disease and applications thereof |
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| JP2017165684A (en) * | 2016-03-16 | 2017-09-21 | 日本曹達株式会社 | Method for preventing rice plant disease |
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Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4274750B2 (en) * | 2002-06-28 | 2009-06-10 | 出光興産株式会社 | Microbial pesticide with good storage stability |
| JP4362584B2 (en) * | 2003-10-28 | 2009-11-11 | タキイ種苗株式会社 | Plant seedling processing apparatus and processing method |
| US8569037B2 (en) * | 2003-10-29 | 2013-10-29 | Kureha Corporation | Fungus having activity of controlling disease of gramineous plant, controlling agent using the same, method of controlling and biological material |
| JP2006124337A (en) * | 2004-10-29 | 2006-05-18 | Idemitsu Kosan Co Ltd | Plant disease controlling agent |
| JP5057542B2 (en) * | 2004-12-01 | 2012-10-24 | 株式会社クレハ | Production method of biological materials or control agents for controlling grass plant diseases |
| WO2006109795A1 (en) * | 2005-04-11 | 2006-10-19 | Kureha Corporation | Method for producing filamentous fungus spores and method for preventing plant disease |
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- 2005-07-22 JP JP2005213033A patent/JP4810151B2/en active Active
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- 2006-07-21 CN CNA2006800267054A patent/CN101227822A/en active Pending
- 2006-07-21 WO PCT/JP2006/314481 patent/WO2007011025A1/en active Application Filing
- 2006-07-21 KR KR1020087004145A patent/KR101280679B1/en active IP Right Grant
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107950263A (en) * | 2011-11-04 | 2018-04-24 | 拜耳知识产权有限责任公司 | Prevent the method for transplanting rice disease |
| CN114025612A (en) * | 2019-06-27 | 2022-02-08 | 出光兴产株式会社 | Plant disease control agent and plant disease control method |
| CN114025612B (en) * | 2019-06-27 | 2023-04-07 | 日本史迪士生物科学株式会社 | Plant disease control agent and plant disease control method |
| CN114456955A (en) * | 2022-02-25 | 2022-05-10 | 中国水稻研究所 | High-efficiency inoculation method for rice bakanae disease and application thereof |
| CN114456955B (en) * | 2022-02-25 | 2024-03-19 | 中国水稻研究所 | Efficient inoculation method for bakanae disease of rice and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2007011025A1 (en) | 2007-01-25 |
| KR101280679B1 (en) | 2013-07-01 |
| KR20080030102A (en) | 2008-04-03 |
| TW200738148A (en) | 2007-10-16 |
| JP4810151B2 (en) | 2011-11-09 |
| TWI379642B (en) | 2012-12-21 |
| JP2007031294A (en) | 2007-02-08 |
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