CN105145632B - It is a kind of with the compound bacteria enzyme system of multiple effect and its application - Google Patents
It is a kind of with the compound bacteria enzyme system of multiple effect and its application Download PDFInfo
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Abstract
The invention discloses a kind of with the compound bacteria enzyme system of multiple effect and its application.Compound bacteria enzyme system provided by the present invention is made up of microbial bacterial agent and organic matter；The active component of microbial bacterial agent is made up of bacillus subtilis (Bacillus subtilis) CGMCC No.1.1630 and Pediococcus acidilactici (Pediococcus acidilactici) CGMCC No.1.2696；Organic matter is humic acid.It is demonstrated experimentally that the compound bacteria enzyme system of the present invention can with coordinate plant growth, improve plant products, preventing and treating plant pest, improve and disease resistance of plant, improved soil and increase soil fertility.
Description
Technical field
The present invention relates to a kind of with the compound bacteria enzyme system of multiple effect and its application in biological technical field.
Background technology
The resultant effect that modern agriculture increasingly focuses on Agrotechnical formulation (exploitation of such as modern fungicides, is sent out plant growth
The facilitation effect educated also turns into an importance of evaluation fungicide effect), to provide crop the protection of Comprehensive,
Ensure the good growth of crop.And the agricultural chemicals applied in modern agriculture, it is typically all " specially anti-specially to control ", this just makes agriculture
Industry applies substantial amounts of, various chemicals in each growth phase of crop, is comprehensively prevented to ensure that crop obtains with this
Shield.But as the method will be such that substantial amounts of chemicals enters in agricultural land soil, serious destruction is caused to soil so that
There is the problems such as drastically declining, the soil salinization, residues of pesticides, heavy metal pollution and then highlighted in the quality of agricultural land soil, unfavorable
In the sustainable development of agricultural, but also a series of problems such as residues of pesticides are exceeded, agricultural product quality declines can be caused.By
This, the exploitation of multipotency composite farm product, also becomes the active demand of modern agriculture.
The content of the invention
The technical problems to be solved by the invention be how coordinate plant growth, improve plant products, preventing and treating plant disease pest
Evil, improve disease resistance of plant, improved soil and increase soil fertility.
In order to solve the above technical problems, present invention firstly provides a kind of compound bacteria enzyme body that can promote plant growth
System, the compound bacteria enzyme system can also reduce plant disease, and with improved soil and can increase soil fertility.
Compound bacteria enzyme system provided by the present invention, is made up of microbial bacterial agent and organic matter；
The active component of the microbial bacterial agent is made up of bacillus subtilis and Pediococcus acidilactici；
The organic matter is humic acid.
In above-mentioned compound bacteria enzyme system, the bacillus subtilis is concretely entrusted in Chinese microorganism strain preservation management
The bacillus subtilis (Bacillus subtilis) that the numbering of member's meeting common micro-organisms center is CGMCC No.1.1630；Institute
It is concretely CGMCC in the numbering of China Committee for Culture Collection of Microorganisms's common micro-organisms center to state Pediococcus acidilactici
No.1.2696 Pediococcus acidilactici (Pediococcus acidilactici).
In above-mentioned compound bacteria enzyme system, the proportioning of microbial bacterial agent and the organic matter described in the compound bacteria enzyme system
It can be the bacillus subtilis：The Pediococcus acidilactici：Humic acid=4 × 109cfu：(2-5)×109cfu：(10-
60) g, such as described bacillus subtilis：The Pediococcus acidilactici：Humic acid=4 × 109cfu：3.5×109cfu：
52.5g。
The ratio of bacillus subtilis and the Pediococcus acidilactici described in the microbial bacterial agent can be the withered grass bud
The number of colony forming units of spore bacillus and Pediococcus acidilactici ratio.
In above-mentioned compound bacteria enzyme system, concretely AlfaAesar Chemical Co., Ltd. product, article No. are the humic acid
41747。
In above-mentioned compound bacteria enzyme system, the active component of the microbial bacterial agent can be the bacillus subtilis and described
After the fermented culture of Pediococcus acidilactici, or directly use or concentrate use or the manufactured viable bacteria product through carrier adsorption.
In above-mentioned compound bacteria enzyme system, microorganism in the microbial bacterial agent can with the zymotic fluid of living cells, trained
Foster living cells, the filtrate of cell culture or cell and the form of the mixture of filtrate are present.In the implementation of the present invention
In example, the bacillus subtilis and the Pediococcus acidilactici can be with the zymotic fluid of living cells, the living cells being cultured, cells
The form of the filtrate of culture or cell and the mixture of filtrate is present.
In order to solve the above technical problems, present invention also offers the microbial bacterial agent.
In order to solve the above technical problems, present invention also offers following M1 or M2 application：
M1, the compound bacteria enzyme system are preparing coordinate plant growth and/or preventing and treating plant pest and/or are improving plant
Application in disease resistance and/or improved soil and/or product of increasing soil fertility；
M2, the microbial bacterial agent are preparing coordinate plant growth and/or preventing and treating plant pest and/or are improving Genes For Plant Tolerance
Application in characteristic of disease and/or improved soil and/or product of increasing soil fertility.
In above-mentioned application, the product can be microbial manure, biological pesticide or soil conditioner.The microbial manure
Can be composite microbiological fertilizer and/or biological organic fertilizer.The composite microbe fertilizer can be that microbial inoculum, nutriment and organic matter are answered
The fertilizer formed is closed, the effect of the existing microorganism of composite microbe fertilizer, there is chemical fertilizer again.The biological organic fertilizer can
A kind of fertilizer being combined for microbial inoculum and decomposed organic fertilizer.The formulation of composite microbiological fertilizer and/or biological organic fertilizer can
For granule.
In above-mentioned application, the plant can be dicotyledon or monocotyledon.The dicotyledon can be muskmelon
(Cucumis melo) or tomato (Lycopersicon esculentum).
The plant pest can be plant powdery mildew, such as melon powdery mildew.
In order to solve the above technical problems, present invention also offers following P1 or P2 product：
P1, the coordinate plant growth prepared using the compound bacteria enzyme system and/or preventing and treating plant pest and/or raising
Disease resistance of plant and/or improved soil and/or the product increase soil fertility；
P2, the coordinate plant growth prepared using the microbial bacterial agent and/or preventing and treating plant pest and/or raising are planted
Thing disease resistance and/or improved soil and/or the product increase soil fertility.
In the said goods, the product can be microbial manure, biological pesticide or soil conditioner.The microbial manure
Can be composite microbiological fertilizer and/or biological organic fertilizer.The composite microbe fertilizer can be that microbial inoculum, nutriment and organic matter are answered
The fertilizer formed is closed, the effect of the existing microorganism of composite microbe fertilizer, there is chemical fertilizer again.The biological organic fertilizer can
A kind of fertilizer being combined for microbial inoculum and decomposed organic fertilizer.The formulation of composite microbiological fertilizer and/or biological organic fertilizer can
For granule.
In the said goods, the plant can be dicotyledon or monocotyledon.The dicotyledon can be muskmelon
(Cucumis melo) or tomato (Lycopersicon esculentum).
The plant pest can be plant powdery mildew, such as melon powdery mildew.
In order to solve the above technical problems, present invention also offers any application in following X1-X6：
X1, the compound bacteria enzyme system in coordinate plant growth and/or preventing and treating plant pest and/or improve plant disease-resistant
Application in property；
The application of X2, the compound bacteria enzyme system in improved soil and/or in increasing soil fertility；
X3, the microbial bacterial agent in coordinate plant growth and/or preventing and treating plant pest and/or improve disease resistance of plant
In application；
The application of X4, the microbial bacterial agent in improved soil and/or in increasing soil fertility；
X5, the product answering in coordinate plant growth and/or preventing and treating plant pest and/or raising disease resistance of plant
With；
The application of X6, the product in improved soil and/or in increasing soil fertility.
In above-mentioned application, the plant can be dicotyledon or monocotyledon.The dicotyledon can be muskmelon
(Cucumis melo) or tomato (Lycopersicon esculentum).
The plant pest can be plant powdery mildew, such as melon powdery mildew.
In the present invention, the coordinate plant growth concretely promotes nutrient growth and/or the reproductive growth of plant.It is described
Nutrient growth can be growth, the growth of stem and/or the growth of fruit of root system.The growth of the root system specifically may be embodied in lateral root
In quantity, the growth of the stem specifically may be embodied in stem diametrically, the specific size that may be embodied in fruit of growth of fruit or
On maturation time.The plant is seed plant, and the reproductive growth may be embodied in fruit maturation time and/or fruit number
On.
In one embodiment of the invention, the regulating plant growth is given birth to for the nutrient growth and reproduction of promotion muskmelon
It is long, it is embodied in muskmelon and bears fruit the increase of quantity, muskmelon annesl fruit number and single fruit weight.In an alternative embodiment of the invention
In, the regulating plant growth to promote the nutrient growth of tomato and reproductive growth, be embodied in the increase of root amount, stem plus
Slightly, in the increase for the amount of bearing fruit and the shortening of annesl time.
The improved soil specifically may be embodied in biological activity of soil, water penetration and gas permeability and improve, described to improve soil
Earth fertility, which specifically may be embodied in, to be promoted on plant root growth.
In the present invention, the formulation of the microbial bacterial agent can be a variety of formulations, as liquor, emulsion, suspending agent, pulvis,
Granula, wettable powder or water dispersible granules.
The microbial bacterial agent may also include carrier.The carrier can be solid carrier and/or liquid-carrier.The solid
Carrier can be mineral material, biomaterial and/or high-molecular compound；The mineral material can be clay, talcum, kaolin,
At least one of montmorillonite, white carbon, zeolite, silica, turfy soil and diatomite；The biomaterial can be the straw of all kinds of crops
At least one of stalk, loose shell, straw, peanut shell, corn flour, bean powder, starch, turf and excrement of animal；The macromolecule
Compound can be polyvinyl alcohol and/or polyglycols.The liquid-carrier can be organic solvent, vegetable oil, mineral oil or water；It is described
Organic solvent is decane and/or dodecane.The clay concretely attapulgite clay.The attapulgite clay is one kind with recessed
Convex rod stone be main component clay mineral, main chemical compositions be hydration zeopan, containing K, Na, Ca, Fe, Al, Mg,
The elements such as Mn, Ti, there is unique dispersibility and adsorption capacity.The active component of the microbial bacterial agent can be to be cultured
Living cells, the zymotic fluid of living cells, the filtrate of cell culture or the form of cell and the mixture of filtrate be present.
As needed, surfactant (such as polysorbas20, Tween 80), bonding can be also added in the microbial bacterial agent
Agent, stabilizer (such as antioxidant), pH adjusting agent.
In one embodiment of the invention, the carrier is concretely by turfy soil, stalk, corn flour and polyvinyl alcohol
According to the turfy soil：The stalk：The corn flour：Polyvinyl alcohol=1.5：3：4：1.5 mass ratio is well mixed
Obtained mixture.The polyvinyl alcohol concretely Aladdin reagent Co., Ltd product, article No. P139550.
In one embodiment of the invention, the active component of microbial bacterial agent described in the compound bacteria enzyme system contains
Measure as 7.5 × 109Cfu/g compound bacteria enzyme system pulvis.
In the present invention, the applicable soil moisture of the composite microbial bacteria enzyme system can be 0-65 DEG C.Described 0-65 DEG C can
For 25-45 DEG C, such as 37 DEG C.The applicable soil moisture of the composite microbial bacteria enzyme system can be 20-100%.It is described compound micro-
The applicable soil pH of biological bacteria enzyme system can be 1.5-9.6, such as 6-8.The applicable soil oxygen of the composite microbial bacteria enzyme system
Gas concentration can be 0.5%-9%.The concentration for being applicable chlorine in soil of the composite microbial bacteria enzyme system may be less than being equal to
3mg/Kg soil.The concentration for being applicable copper oxychloride in soil of the composite microbial bacteria enzyme system may be less than being equal to 5mg/Kg
Soil.The concentration for being applicable soil sulfite sodium of the composite microbial bacteria enzyme system may be less than being equal to 250mg/Kg soil
Earth.The concentration for being applicable organophosphor in soil of the composite microbial bacteria enzyme system may be less than being equal to 30mg/Kg soil.It is described
The concentration for being applicable soil glyphosate of composite microbial bacteria enzyme system may be less than being equal to 50mg/Kg soil.It is described compound micro-
The concentration for being applicable dichlorphenoxyacetic acid in soil of biological bacteria enzyme system may be less than being equal to 8000mg/Kg soil.It is described compound
The concentration for being applicable benzalkonium chloride in soil of microbial bacteria enzyme system may be less than being equal to 200mg/Kg soil.
It is demonstrated experimentally that the compound bacteria enzyme system of the present invention can be with coordinate plant growth, raising plant products, preventing and treating phytopathy
Insect pest, improve disease resistance of plant, improved soil and increase soil fertility：Compared with control treatment area, the processing of compound bacteria enzyme system
The number of bearing fruit of muskmelon, annesl fruit number, the amplification of fruit average weight be respectively 37.14%, 44%, 8.33%；With compareing place
Reason area compares, the tomato stem of compound bacteria enzyme system processing slightly and Sansui fruit bear fruit several amplification be respectively 18.29%,
27.97%；Compared with control treatment area, tomato the first fringe fruit annesl time of compound bacteria enzyme system processing averagely shifts to an earlier date 5 days, and
And there is significant change the tomato root of pulvis treatment region, root amount is more, and lateral root is more flourishing；It is compound compared with control treatment area
The melon powdery mildew morbidity strain number of bacterium enzyme system processing reduces, in pouring root the 20th day, the melon powdery mildew morbidity of pulvis treatment region
Strain number is the 50% of control treatment area；Compared with control treatment area, the hydrolase in compound bacteria enzyme system pulvis treatment region soil
The content of (proteolytic enzyme, cellulolytic enzyme) improves 20%.
The composite microbial bacteria enzyme system of the present invention, which has, to be promoted plant growth, improves plant products, preventing and treating plant disease pest
Evil, improve disease resistance of plant, improved soil and the multiple effect such as increase soil fertility, multiple-protection can be provided for crop, can be subtracted
Few agricultural chemicals include the amount of application of agricultural chemicals；The composite microbial bacteria enzyme system of the present invention is pure natural organic preparation, to people
Body is harmless, free from environmental pollution, is advantageous to the sustainable development of agricultural；Beneficial bacterium in the composite microbial bacteria enzyme system of the present invention
Kind can be colonized survival after being manured into soil, rehabilitating soil environment, to promoting plant growth, reduce crop disease and occur to have actively
Effect.The composite microbial bacteria enzyme system of the present invention provides integrated protection for modern agriculture crop, to reduce harmful chemicals
Use, meet the needs of Sustainable Development of Modern Agriculture.
Embodiment
The present invention is further described in detail with reference to embodiment, the embodiment provided is only for explaining
The bright present invention, the scope being not intended to be limiting of the invention.
Experimental method in following embodiments, it is conventional method unless otherwise specified.
Material used, reagent etc., unless otherwise specified, are commercially obtained in following embodiments.
Bacillus subtilis in following embodiments is bacillus subtilis (Bacillus subtilis) CGMCC
No.1.1630, bacillus subtilis (Bacillus subtilis) CGMCC No.1.1630 are preserved in March 1 nineteen eighty-three
China Committee for Culture Collection of Microorganisms's common micro-organisms center (abbreviation CGMCC), the public can be therefrom from the preservation day
State's Microbiological Culture Collection administration committee common micro-organisms center obtains the bacterial strain.
Pediococcus acidilactici in following embodiments is Pediococcus acidilactici (Pediococcus acidilactici) CGMCC
No.1.2696, Pediococcus acidilactici (Pediococcus acidilactici) CGMCC No.1.2696 were on November 23rd, 2000
China Committee for Culture Collection of Microorganisms's common micro-organisms center (abbreviation CGMCC) is preserved in, the public from the preservation day
The bacterial strain can be obtained from China Committee for Culture Collection of Microorganisms's common micro-organisms center.
No. 1 culture medium in following embodiments is the jade that China General Microbiological culture presevation administrative center numbering is 2006
Rice agar medium.
No. 2 culture mediums in following embodiments are that China General Microbiological culture presevation administrative center numbering is 0006
MRS culture mediums.
Muskmelon (Cucumis melo) in following embodiments is emerald green precious for white jade, is according to agriculture Products, catalog number
For W08.
Tomato (Lycopersicon esculentum) in following embodiments is good for gold honey, is according to agriculture Products, production
Product catalog number (Cat.No.) is YNTR.
Humic acid in following embodiments is AlfaAesar Chemical Co., Ltd. product, article No. 41747.
Polyvinyl alcohol in following embodiments is Aladdin reagent Co., Ltd product, article No. P139550.
Embodiment 1, compound bacteria enzyme system different dosage forms preparation
1st, the activation of bacterium is cultivated with expanding
Activation：Bacillus subtilis is seeded on No. 1 culture medium, 30 DEG C of incubator cultures 3 days is placed in, is activated
Bacillus subtilis；Pediococcus acidilactici is seeded on No. 3 culture mediums, is placed in 30 DEG C of incubator cultures 3 days, the breast activated
Sour piece coccus.
Expand culture：The Pediococcus acidilactici of the bacillus subtilis of above-mentioned activation and activation is respectively connected to two difference
Equipped with No. 1 culture medium, No. 2 culture mediums fermentation tank in, carried out at 30 DEG C it is static expand culture step by step, expand in incubation
Inoculum concentration is 5%-10% (volume percent content), respectively obtains bacillus subtilis fermentation liquor (1 × 109Cfu/ml it is) and newborn
Sour piece coccus zymotic fluid (1 × 109cfu/ml)。
2nd, the preparation of compound bacteria enzyme system aqua and pulvis
By the bacillus subtilis fermentation liquor obtained according to step 1 and the CFU of Pediococcus acidilactici zymotic fluid
(cfu) number ratio is 4:3.5 ratio carries out being mixed to get fermentation mixed liquor, bacillus subtilis and breast in the fermentation mixed liquor
The content of sour piece coccus is respectively 8 × 107cfu/ml、7×107cfu/ml。
The preparation of organic matter solution：Xiang Shuizhong adds humic acid, and obtaining humic acid concentration, (mass percent is dense for 35%
Degree) liquid.
The preparation of compound bacteria enzyme system aqua：Above-mentioned fermentation mixed liquor is mixed with organic matter solution, obtains compound bacteria enzyme
System aqua, the content of the microbial bacterial agent in compound bacteria enzyme system aqua is 1 × 108Cfu/g compound bacteria enzyme system aquas, its
In, CFU (cfu) the number ratio of bacillus subtilis and Pediococcus acidilactici is 4:3.5.
The preparation of compound bacteria enzyme system pulvis：By absorption carrier (absorption carrier be by turfy soil, stalk, corn flour and
Polyvinyl alcohol is according to turfy soil：Stalk：Corn flour：Polyvinyl alcohol=1.5：3：4：1.5 mass ratio is well mixed obtain it is mixed
Compound) and the above-mentioned fermentation mixed liquors of 50mL mixed with the above-mentioned organic matter solution of 150mL, holding total moisture content is 75% (quality hundred
Point ratio), after abundant absorption, 35 DEG C naturally ventilated to be dried to obtain compound bacteria enzyme system pulvis, compound bacteria for 30 DEG C of static gas wave refrigerators 3 days
The content of microbial bacterial agent in enzyme system pulvis is 7.5 × 109Cfu/g compound bacteria enzyme system pulvis, wherein, bacillus subtilis
The CFU of bacterium and Pediococcus acidilactici (cfu) number ratio is 4:3.5.
Embodiment 2, compound bacteria enzyme system can improve the yield and disease resistance of muskmelon
Field experimental design is as follows：Experiment uses RANDOMIZED BLOCK DESIGN, sets 3 duplicate blocks, each duplicate block is set at random
Put 2 treatment regions, respectively pulvis treatment region and control treatment area.The area of each treatment region is 0.5 mu, each treatment region
Growth has the muskmelon (Cucumis melo) of 15 days after 10 plants of emergences.
In the present embodiment, field experiment soil moisture is 16%-18%, and temperature is 20-30 DEG C.
1st, pulvis treatment region
Following handle is carried out to pulvis treatment region：By compound bacteria enzyme system pulvis suspension, (compound bacteria enzyme system pulvis hangs
Supernatant liquid is that the compound bacteria enzyme system pulvis of 200g embodiments 1 is added in 150 liters of water to obtained suspension) pouring root administration, every plant
Muskmelon 150mL compound bacteria enzyme system pulvis suspension, once, each every plant of muskmelon compound bacteria enzyme system pulvis hangs every 15 days pouring roots
The volume of supernatant liquid pouring root is 150mL compound bacteria enzyme system pulvis suspension.
The number of bearing fruit for period statistics muskmelon of bearing fruit is grown in muskmelon；Fruit maturation period statistics muskmelon is grown in muskmelon
Annesl fruit number；In muskmelon maturation, the weight of fruit is counted；Opened within first day from compound bacteria enzyme system pulvis suspension liquid irrigating root
Begin, the continuous 20 days morbidity strain numbers (table 1) for counting melon powdery mildew daily.
Muskmelon grow to bear fruit period using conventional method measure muskmelon root soil in micro organism quantity and bacterium enzyme
Content.
2nd, control treatment area
To control treatment area, according to the processing method of above-mentioned pulvis treatment region, compound bacteria enzyme system pulvis suspension is replaced
Water is changed to, other are applied as usual using the field management method, conventional pesticide, chemical fertilizer, farm manure etc. of peasant household's custom.
The number of bearing fruit for period statistics muskmelon of bearing fruit is grown in muskmelon；Turning for mature period statistics muskmelon is grown in muskmelon
Color fruit number；In muskmelon maturation, the weight of fruit is counted；From water pouring root daystart, it is white muskmelon to be counted daily within continuous 20 days
The morbidity strain number (table 1) of powder disease.
Muskmelon grow to bear fruit period using conventional method measure muskmelon root soil in micro organism quantity and hydrolysis
Enzyme content.
As a result showing, the number of bearing fruit of the muskmelon of pulvis treatment region, annesl fruit number and fruit average weight be respectively 4.8 ±
0.4th, 3.6 ± 0.489,0.26, the number of bearing fruit of the muskmelon in control treatment area, annesl fruit number, fruit average weight be respectively 3.5 ±
1.667th, 2.5 ± 1.389,0.24, compared with control treatment area, bear fruit number, annesl fruit number, the fruit of the muskmelon of pulvis treatment region
The amplification of average weight is respectively 37.14%, 44%, 8.33%.Show, compound bacteria enzyme system can promote melon fruit into
It is ripe, the average weight raising melon yield of can also be beared fruit by increase number and fruit.
As a result show, pulvis treatment region muskmelon root microbial total amount is control treatment area muskmelon root microbial total amount
2.3 again.
The average onset strain number (strain) of table 1, melon powdery mildew
| Time | Pulvis treatment region | Control treatment area |
| 1st day | 0 | 0 |
| 2nd day | 0 | 0 |
| 3rd day | 1 | 1 |
| 4th day | 1 | 1 |
| 5th day | 1 | 1 |
| 6th day | 1 | 2 |
| 7th day | 1 | 2 |
| 8th day | 1 | 3 |
| 9th day | 1 | 3 |
| 10th day | 1 | 4 |
| 11st day | 1 | 4 |
| 12nd day | 2 | 4 |
| 13rd day | 2 | 5 |
| 14th day | 2 | 5 |
| 15th day | 2 | 5 |
| 16th day | 2 | 6 |
| 17th day | 2 | 6 |
| 18th day | 2 | 6 |
| 19th day | 2 | 6 |
| 20th day | 3 | 6 |
Compared with control treatment area, the melon powdery mildew of pulvis treatment region morbidity strain number reduces, in pouring root the 20th day, pulvis
The melon powdery mildew morbidity strain number for the treatment of region is the 50% of control treatment area, is shown, pulvis can reduce the hair of melon powdery mildew
Sick rate, illustrates, compound bacteria enzyme system can improve the disease resistance of muskmelon.
Hydrolase (proteolytic enzyme and cellulolytic enzyme) content in pulvis treatment region soil is about every gram of soil
In 500,000 units, the content of the hydrolase in control treatment area soil is about 400,000 units in every gram of soil, with control treatment area
Compare, the content of the hydrolase in pulvis treatment region soil improves 20%.
Embodiment 3, compound bacteria enzyme system can improve the yield and disease resistance of tomato
Field experimental design is as follows：Experiment uses RANDOMIZED BLOCK DESIGN, sets 3 duplicate blocks, each duplicate block is set at random
Put 2 treatment regions, respectively pulvis treatment region and control treatment area.The area of each treatment region is 0.5 mu, each treatment region
4 days application processes according to conventional organic fertilizer are applied organic before tomato (Lycopersicon esculentum) Seeding planting
Fertilizer, and plough；Before tomato (Lycopersicon esculentum) Seeding planting 2 days by 150g compound bacteria enzyme system pulvis
Add in 60L warm water and stand 12 hours to activate medicament, the pulvis aqueous solution after being activated.
In the present embodiment, field experiment soil moisture is 16%-18%, and temperature is 20-30 DEG C.
1st, pulvis treatment region
Following handle is carried out to pulvis treatment region：By the pulvis aqueous solution after activation, uniformly spray arrives native table, and gently turns over and make
The soil layer for the pulvis aqueous solution being soaked with after activation is located at the soil horizon away from earth's surface 0-15cm, after 36 hours, is colonized tomato seedling, will
The field planting same day is designated as field planting the 0th day；In field planting the 4th day and the 27th day, 150mL compound bacteria enzymes are sprayed to every plant of tomato seedling respectively
System aqua dilution (compound bacteria enzyme system aqua dilution be by compound bacteria enzyme system aqua dilution 200 times obtain it is molten
Liquid)；In field planting the 29th day, the pulvis aqueous solution after the above-mentioned activation of 150mL is applied to the root of every plant of tomato.
It is thick that the 13rd day stem measured at the liftoff 15cm of every plant of tomato plant is colonized in tomato；It is colonized the 45th day and counts in tomato
The number of bearing fruit of tomato Sansui fruit；Record the annesl time of tomato the first fringe fruit and observe the growth shape of tomato plant root root
Condition.
It is colonized the 45th day in tomato and is contained using the micro organism quantity in conventional method measure muskmelon root soil and hydrolase
Amount.
2nd, control treatment area
To control treatment area, according to the processing method of above-mentioned pulvis treatment region, the pulvis aqueous solution after activation is replaced with
Water, and compound bacteria enzyme system aqua dilution is replaced with into water, other are using the field management method of peasant household's custom, conventional agriculture
Medicine, chemical fertilizer, farm manure etc. are applied as usual.
Stem after tomato is colonized at the 13rd day liftoff 15cm of measurement every plant of tomato plant of measurement is thick；The after tomato is colonized
The number of bearing fruit of 45 days statistics tomato Sansui fruits；Record the annesl time of tomato the first fringe fruit and observe tomato plant root root
Upgrowth situation.
It is colonized the 45th day in tomato and is contained using the micro organism quantity in conventional method measure muskmelon root soil and hydrolase
Amount.
As a result show, the tomato stem of pulvis treatment region slightly and Sansui fruit bear fruit number be respectively 0.5604 ± 0.00733cm,
7.55 ± 8.79, the tomato stem in control treatment area is slightly beared fruit with Sansui fruit counts respectively 0.4737 ± 0.00356cm, 5.90
± 6.31, compared with control treatment area, the tomato stem of pulvis treatment region is slightly and Sansui fruit several amplification of bearing fruit is respectively
18.29%th, 27.97%；Compared with control treatment area, tomato the first fringe fruit annesl time of pulvis treatment region averagely shifts to an earlier date 5 days,
And there is significant change the tomato root of pulvis treatment region, and root amount is more, and lateral root is more flourishing.Show, compound bacteria enzyme system can
To promote the maturation of the nutrient growth of tomato and fruit, the number that can also be beared fruit by increase improves tomato yield.
The content of hydrolase (proteolytic enzyme, cellulolytic enzyme) in treatment region soil is about 50 in every gram of soil
Ten thousand units, the content of the hydrolase (proteolytic enzyme, cellulolytic enzyme) in control treatment area soil is about every gram of soil
In 500,000 units, compared with control treatment area, hydrolase in pulvis treatment region soil (proteolytic enzyme, cellulose hydrolysis
Enzyme) content improve 20%.
The technical conditions of composite microbial bacteria enzyme system such as table 2.
The technical conditions of table 2, composite microbial bacteria enzyme system
| Project | Condition |
| Acceptable temperature | 0-65 DEG C in extensive range |
| Preferable temperature | 25-45 DEG C (37 DEG C are optimal) |
| Humidity | 20-100% |
| pH | (1.5-9.6 6-8 is optimal) |
| Nutrition | Humic acid |
| Oxygen | Concentration range is 0.5%-9% |
| Salinity | ≤ 1% |
| Chlorine | ≤3mg/Kg |
| Oxygenated chlorinated copper | ≤5mg/Kg |
| Sodium sulfite | ≤250mg/Kg |
| Organophosphor | ≤30mg/Kg |
| Glyphosate | ≤50mg/Kg |
| Dichlorphenoxyacetic acid (2,4-D) | ≤8000mg/Kg |
| Benzalkonium chloride (BKC) | ≤200mg/Kg |
Claims (13)
1. compound bacteria enzyme system, is made up of microbial bacterial agent and organic matter；
The active component of the microbial bacterial agent is made up of bacillus subtilis and Pediococcus acidilactici；
The organic matter is humic acid；
The bacillus subtilis is bacillus subtilis (Bacillus subtilis) CGMCC No.1.1630；The lactic acid
Piece coccus is Pediococcus acidilactici (Pediococcus acidilactici) CGMCC No.1.2696.
2. compound bacteria enzyme system according to claim 1, it is characterised in that：Microorganism described in the compound bacteria enzyme system
The proportioning of microbial inoculum and the organic matter is the bacillus subtilis：The Pediococcus acidilactici：Humic acid=4 ×
109cfu：(2-5)×109cfu：(10-60)g.
3. the compound bacteria enzyme system of claim 1 or 2 prepare coordinate plant growth and/or preventing and treating plant pest and/or
Improve the application in disease resistance of plant and/or improved soil and/or product of increasing soil fertility.
4. application according to claim 3, it is characterised in that：The product is microbial manure, biological pesticide or soil
Modifying agent.
5. the application according to claim 3 or 4, it is characterised in that：The plant is dicotyledon or monocotyledon；
The plant pest is plant powdery mildew.
6. the coordinate plant growth and/or preventing and treating plant pest that are prepared using the compound bacteria enzyme system of claim 1 or 2
And/or improve disease resistance of plant and/or improved soil and/or the product increase soil fertility.
7. the product described in claim 6, it is characterised in that：The product is microbial manure, biological pesticide or soil improvement
Agent.
8. the product according to claim 6 or 7, it is characterised in that：The plant is dicotyledon or monocotyledon；
The plant pest is plant powdery mildew.
9. the compound bacteria enzyme system of claim 1 or 2 is in coordinate plant growth and/or preventing and treating plant pest and/or improve
Application in disease resistance of plant.
10. application of the compound bacteria enzyme system of claim 1 or 2 in improved soil and/or in increasing soil fertility.
11. any product in coordinate plant growth and/or preventing and treating plant pest and/or improves plant in claim 6-8
Application in thing disease resistance.
12. application of any product in improved soil and/or in increasing soil fertility in claim 6-8.
13. according to any described applications of claim 9-12, it is characterised in that：The plant is dicotyledon or unifacial leaf
Plant；The plant pest is plant powdery mildew.
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| CN201510428143.7A CN105145632B (en) | 2015-07-20 | 2015-07-20 | It is a kind of with the compound bacteria enzyme system of multiple effect and its application |
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| CN111848302A (en) * | 2019-12-17 | 2020-10-30 | 中国农业大学 | Application of chitosan functionalized nano-selenium composite bacterium enzyme system in improvement of yield and quality of Chinese herbal medicine |
| CN111848303A (en) * | 2019-12-17 | 2020-10-30 | 中国农业大学 | Application of chitosan functionalized nano-selenium composite bacterium enzyme system in improvement of tea yield and quality |
| CN113396937A (en) * | 2020-03-17 | 2021-09-17 | 孟州云大高科生物科技有限公司 | Composition of multienzyme and biological bactericide |
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| CN104320980A (en) * | 2012-11-28 | 2015-01-28 | J9生物农业协会公司 | Health supplement food containing pediococcus acidilactici j9, and method for manufacturing same |
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| CN104611248A (en) * | 2014-11-11 | 2015-05-13 | 角志海 | Composite biological living bacteria preparation and preparation technology thereof |
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