CN106857674A - The application of Methylotrophic bacillus BH21 and its microbial manure and preparation method - Google Patents
The application of Methylotrophic bacillus BH21 and its microbial manure and preparation method Download PDFInfo
- Publication number
- CN106857674A CN106857674A CN201710070295.3A CN201710070295A CN106857674A CN 106857674 A CN106857674 A CN 106857674A CN 201710070295 A CN201710070295 A CN 201710070295A CN 106857674 A CN106857674 A CN 106857674A
- Authority
- CN
- China
- Prior art keywords
- methylotrophic bacillus
- microbial manure
- plant
- growth
- bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 37
- 210000003608 fece Anatomy 0.000 title claims abstract description 30
- 239000010871 livestock manure Substances 0.000 title claims abstract description 23
- 230000000813 microbial effect Effects 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 150000003839 salts Chemical class 0.000 claims abstract description 24
- 230000008635 plant growth Effects 0.000 claims abstract description 12
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 11
- 244000144972 livestock Species 0.000 claims abstract description 7
- 244000144977 poultry Species 0.000 claims abstract description 7
- 230000001737 promoting effect Effects 0.000 claims abstract description 6
- 230000015784 hyperosmotic salinity response Effects 0.000 claims abstract description 5
- 241000196324 Embryophyta Species 0.000 claims description 20
- 241000894006 Bacteria Species 0.000 claims description 16
- 239000002893 slag Substances 0.000 claims description 10
- 244000061456 Solanum tuberosum Species 0.000 claims description 8
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 8
- 239000004480 active ingredient Substances 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 2
- 239000003895 organic fertilizer Substances 0.000 abstract description 10
- 230000012010 growth Effects 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 9
- 230000001580 bacterial effect Effects 0.000 abstract description 7
- 238000000855 fermentation Methods 0.000 abstract description 7
- 230000004151 fermentation Effects 0.000 abstract description 7
- 235000016709 nutrition Nutrition 0.000 abstract description 6
- 230000035764 nutrition Effects 0.000 abstract description 6
- 230000004083 survival effect Effects 0.000 abstract description 2
- 240000008067 Cucumis sativus Species 0.000 description 18
- 239000002689 soil Substances 0.000 description 18
- 238000011282 treatment Methods 0.000 description 18
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 17
- 240000003768 Solanum lycopersicum Species 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- 238000010790 dilution Methods 0.000 description 7
- 239000012895 dilution Substances 0.000 description 7
- 239000003337 fertilizer Substances 0.000 description 7
- 244000005700 microbiome Species 0.000 description 6
- 230000003020 moisturizing effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 239000002585 base Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000002015 leaf growth Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- WZLMXYBCAZZIRQ-UHFFFAOYSA-N [N].[P].[K] Chemical compound [N].[P].[K] WZLMXYBCAZZIRQ-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229910052602 gypsum Inorganic materials 0.000 description 1
- 239000010440 gypsum Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Pest Control & Pesticides (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses preserving number for the Methylotrophic bacillus BH21 of CGMCC No.11840 is preparing the application having in the microbial manure of growth promoting and raising plant salt endurance, and disclose the microbial manure and include feces of livestock and poultry, mushroom residue, cultivation bedding and padding and Methylotrophic bacillus BH21, its preparation method is：After Methylotrophic bacillus BH21, the excrement that becomes thoroughly decomposed are mixed, fermented using bar formula stacking fermentation method, continuously fermented 45 days, daily turning in fermentation process, control heap temperature is no more than 45 DEG C, after the completion of fermentation, obtains described microbial manure.Bacterial strain of the present invention has stronger salt tolerance, and plant establishment and growth can be promoted, itself and decomposed manure are combined into biological organic fertilizer and can provide abundant nutrition for plant growth, and the nutrition of organic fertilizer is also the survival of function stem and colonizes there is provided good nutrition.
Description
Technical field
The present invention relates to microorganism fungus kind applied technical field, and in particular to a kind of Methylotrophic bacillus BH21's
Using, and microbial manure prepared therefrom and preparation method thereof.
Background technology
In recent years, China's facility plantation development is rapid.410.9 ten thousand hectares of China installment agriculture growth area in 2015, accounts for
85% or so of world's industrialized agriculture gross area.But with the fast development of facility cultivation, long-term ground mulching cultivation makes soil
The physicochemical character and biological characteristics of earth have a very large change, and especially the earth's surface accumulation phenomenon of soil salt is substantially, native
Earth secondary salinization problem is universal.There is the soil of secondary salinization in addition to the growing of Restraining culture crop, also result in it
The decline of yield and quality.Soil in protected field secondary salinization occurs one of soil swelling-shrinkage in having turned into current facility vegetable cultivation
Main Factors.How the agrological production capacity that is healthy, improving secondary salinization soil of support facility is to ensure that China's agriculture
The key of industry sustainable development.
Microorganism is the important activity factor in soil environment, is improving soil microenvironment, is improving crop production energy
Power aspect has important effect.It is effective to screen the bacterial strain with raising plant salt tolerance and growth ability and develop with the bacterial strain
The biological organic fertilizer of composition, it is used in industrialized agriculture production, is reduced because salt content is high in facility canopy planting soil
The crop growth for causing suppresses, and reaches the effect for promoting plant growth, improving plant salt endurance.
The application for a patent for invention of Application No. 201610720403.2《A kind of salt tolerant bacterium T5S2 and its in microbial manure
In application》Disclose cotton, the salt reduction of corn that the microbial manure that salt tolerant bacterium T5S2 formed plants to salt-soda soil, increase
Product is acted on.The application for a patent for invention of Application No. 201410623410.1《A kind of salt-durable microbe flora and its application》Disclose
A kind of application of salt tolerant compounding microorganism species in the waste drilling mud and oily sludge for the treatment of high salt alkali content.Application number
It is 201310466328.8 application for a patent for invention《A kind of method that microorganism improves salt affected soil》Disclose a kind of using multiple
Close the method that microbial bacterial agent improves salt affected soil.
However, at present on Methylotrophic bacillus as salt tolerant bacterium carry out facility salinization soil improvement and
Research in terms of plant growing is less, does not have also to the research with promotion plant growth and the Methylotrophic bacillus for taking root
Have.
The content of the invention
Based on the current saliferous present situation of China's soil in protected field, it is an object of the invention to provide Methylotrophic bacillus
Applications of the BH21 in terms of promoting plant growth, taking root and improve salt resistance ability, and it is the micro- of active ingredient to provide by the bacterial strain
Bio-feritlizer and preparation method thereof, and it is used for the plantation of alkaline land improving and industrialized agriculture.
The technical solution adopted by the present invention is as follows：
Applications of the Methylotrophic bacillus BH21 in promoting plant growth and improving plant salt endurance, is mainly reflected in rush
Enter plant establishment and improve the growth of plant in the case of salt stress.The Methylotrophic bacillus BH21 is in December, 2015
Preservation was carried out in China Committee for Culture Collection of Microorganisms's common micro-organisms center in 09th, preserving number is CGMCC
No.11840, and have submitted patent of invention Shen to Patent Office of Intellectual Property Right Bureau of the RPC on 03 07th, 2016
Please, the .1 of Application No. 201610126777.
Preparing with promotion plant growth and improving plant present invention also offers Methylotrophic bacillus BH21
Application in the microbial manure of salt tolerance, it is preferable that the bacterium of Methylotrophic bacillus BH21 in the microbial manure
Number is more than or equal to 100,000,000 cfu/ grams.
Further, present invention also offers the microbial manure comprising Methylotrophic bacillus BH21, it includes
Following active ingredient：Feces of livestock and poultry, potato slag, mushroom residue, wherein cultivation bedding and padding and Methylotrophic bacillus BH21, mass ratio
Feces of livestock and poultry：Potato slag+mushroom residue+cultivation bedding and padding=50-60：40-50, potato slag, mushroom residue and cultivation bedding and padding can suit measures to local conditions, and press
According to arbitrary proportion mixing, the bacterium number of Methylotrophic bacillus BH21 is more than or equal to 100,000,000 cfu/ grams in the microbial manure.
Further, present invention also offers the preparation method of mentioned microorganism fertilizer, i.e.,：The feces of livestock and poultry, potato
Slag, mushroom residue and cultivation bedding and padding are become thoroughly decomposed, and Methylotrophic bacillus is added when the heap temperature drop that becomes thoroughly decomposed is to less than 45 DEG C
BH21, once, control heap temperature obtains final product the microbial manure below 45 DEG C, after 4-5 days for daily turning.
The beneficial effects of the invention are as follows：The invention provides the new opplication of Methylotrophic bacillus BH21, the bacterial strain
Plant establishment and growth can be promoted, and the salt tolerance of plant can be improved.In addition, the bacterial strain can be big in salt affected soil
Amount breeding, forms dominant microflora, so as to promote plant nutrient to absorb, increases plant products.In addition, the bacterium and decomposed manure group
Synthesising biological organic fertilizer can provide abundant nutrition for plant growth, and the nutrition of organic fertilizer also for function stem survival and
Colonize there is provided good nutrition.
Specific embodiment
Rush rooting effects of the Methylotrophic bacillus BH21 of embodiment 1 in vitro cucumber leaves
The close healthy leaves of size is won from healthy cucumber plant, with 4 times, 5 times and 10 times of first of distilled water prepared and diluted
Base auxotype bacillus BH21 bacterium solutions, cucumber leaves are immersed after soaking 5 min in dilution, are dried；With distillation water-soaked
The cucumber leaves for drying are compared.Treated cucumber leaves are placed on the culture dish for being lined with moistening gauze and appropriate distilled water
In, leaf growth state is observed in 22 DEG C of moisturizing cultures.
After moisturizing 4 days, all gone out with the cucumber leaves petiole base of Methylotrophic bacillus BH21 fermentation liquor treatments
Now take root phenomenon, wherein the most obvious to dilute 4 times of zymotic fluid rooting efficiency, number of averagely taking root is 12, and root is long
21.5cm；The cucumber leaves of the fermentation liquor treatment of 5 times of dilution are averagely taken root number 6, root 12.3cm long；The zymotic fluid of 10 times of dilution
Process number of taking root few, and root is shorter, only 5.8cm.This is probably because Methylotrophic bacillus BH21 grew
Cheng Zhonghui produces some bioactivators, is taken root so as to the petiole for promoting cucumber leaves is induced during moisturizing.
In addition, the leaf in vitro of zymotic fluid immersion is after 7 days, leaf color is still bud green, vane extension, and clear water control treatment leaf color is partially yellow
And wilt, treatment of aged state.
Rush rooting effects of the Methylotrophic bacillus BH21 of embodiment 2 in vitro tomato leaf
The close healthy leaves of size is won from healthy tomato plant, is sought with 5 times and 10 times of methyl of distilled water prepared and diluted
The type of supporting bacillus BH21 bacterium solutions, tomato leaf are immersed after soaking 5 min in dilution, are dried；Dried with distillation water-soaked
Tomato leaf compare.Tomato leaf is placed on and is lined with the culture dish of moistening gauze and appropriate distilled water, 22 DEG C of moisturizing trainings
Support, observe leaf growth state.
After moisturizing 4 days, with the tomato leaf of 5 times and 10 times fermentation liquor treatments of dilution of Methylotrophic bacillus BH21
Piece leaf color is bud green, open and flat, and all treatment do not occur phenomenon of taking root.Observation tomato petiole base finds that 5 times dilute during moisturizing 7 days
The tomato leaf for releasing liquid and 10 times of dilution treatment occurs in that fibrous root, and the fibrous root number of 5 times of dilution treatment is more, it is strong to grow
It is strong, and clear water control then grows without fibrous root.Contrast different disposal leaf color finds that the tomato leaf leaf color of clear water treatment is yellow,
And it is bud green to also maintains blade with the tomato leaf that 5 times and 10 times dilutions of bacterium solution process 7 days.
Influences of the Methylotrophic bacillus BH21 of embodiment 3 to plant salt endurance
Using hole plate seedling growth method, by the cucumber seeds of the green small garden in Tianjin, the nursery in greenhouse is long to the heart stage of 1 leaf 1 to seedling, seedling
Root clean up immigration water planting box（Box 20cm long, width 15cm, 10cm high）In, thickness is used for the cystosepiment of 1cm and at it
The circular hole of upper drill diameter 0.5cm, cucumber seedling is planted onto cystosepiment, and each water planting box plants 9 plants of cucumber, evenly distributed.
MM nutrient solutions are filled it up with water planting box, whne cucumber it is long to two leaves wholeheartedly when carry out the treatment of salt and bacterium.Experiment sets four treatment altogether, locates
Reason 1 is the NaCl of 100mmol/L to contain concentration in MM nutrient solutions；Treatment 2 is 100mmol/L to contain concentration in MM nutrient solutions
NaCl, simultaneously containing concentration be 5 × 106The BH21 of cfu/mL；Treatment 3 is MM nutrient solutions.Cucumber is in plant culturing room（Light：
Dark cycle=12：12,24 ± 2 DEG C of temperature）Middle culture 14 days, salt gypsum rock and growing state to cucumber are measured.Result is shown in
Table 1, by result it can be seen that addition BH21 can significantly reduce harm of the salt to cucumber.
Influences of the BH21 of table 1 to salt resistance of plants
。
Bio-organic fertilizer and preparation method of the embodiment 4 with Methylotrophic bacillus BH21 as function stem
The culture of Methylotrophic bacillus BH21 uses patent（A kind of Methylotrophic for producing various lipopeptid class antibiotics
Bacillus and its apply 201610126777.1）Culture medium and cultural method, fermented using multistage fermentation tank, pass through
It is 5 × 10 that spray drying obtains viable count10Cfu/ grams of bacterium powder Cord blood is standby.
Bio-organic fertilizer is comprised the following steps that：By pig manure, flat mushroom slag（Carbon-nitrogen ratio 33:1）, pig bedding and padding（Bedding and padding are former
Expect be：Sawdust+maize straw, pig uses rear carbon-nitrogen ratio 30:1）In mass ratio 50：30：20 are mixed, and adjust moisture
In 60%-70%, decomposing microbial inoculum is added by 1% inoculum concentration, carry out high temperature and become thoroughly decomposed, heap temperature is reduced to less than 45 DEG C afterwards by quality
Additional proportion is 0.1% BH21 bacterium powders, and bacterium powder is diluted with 5% molasses, is uniformly sprayed onto on stacking with sprayer, carried out afterwards
Turning, once, turning 5 days altogether, the quantity of the BH21 in detection fertilizer reaches 1.6 × 10 for daily turning8Cfu/ grams.
Application of the bio-organic fertilizer containing BH21 of embodiment 5 in soil salinization brooder
The application of BH21 bio-organic fertilizers, mu consumption 200kg, before site preparation are carried out in the plastic warm shed of continuous plantation 5 years
Using and turn over into soil.Application test sets 3 treatment altogether：Treatment 1 applies BH21 biological organic fertilizer 200kg for mu, and 2 mu for the treatment of is applied
The kg of organic fertilizer 200 without BH21, treatment 3 is conventional compound fertilizer（Nitrogen-phosphorus-potassium compound fertilizer）Control.To cucumber in growing process
Growing state and yield observed and determined, the results are shown in Table 2.It can be seen from the results that BH21 biological organic fertilizers are in soil
Applied in the saliferous brooder of generation, cucumber growth and output increased can be effectively promoted.
Influence of the BH21 biological organic fertilizers of table 2 to cucumber growth and yield
。
The present invention only lists 5 embodiments to illustrate its content, and not to the exhaustion of implementation method.For art
It is any without creative improvement on the basis of the above description for technical staff, also all in protection category of the invention
Among.There is no need and unable to be exhaustive to all of implementation method.Therefore, without departing from the spirit and scope of the invention
The impartial conversion and modification made, all should be contained within the scope of the invention.
Claims (8)
1. preserving number is the Methylotrophic bacillus BH21 of CGMCC No.11840 in promotion plant growth and raising plant
Application in salt tolerance.
2. application according to claim 1, it is characterised in that：The Methylotrophic bacillus BH21 can promote to plant
Thing is taken root and improves the growth of plant in the case of salt stress.
3. preserving number for CGMCC No.11840 Methylotrophic bacillus BH21 prepare have promote plant growth and
Improve the application in the microbial manure of plant salt endurance.
4. application according to claim 3, it is characterised in that：Methylotrophic bacillus in the microbial manure
The bacterium number of BH21 is more than or equal to 100,000,000 cfu/ grams.
5. a kind of with the microbial manure for promoting plant growth and raising plant salt endurance, it is characterised in that：Wherein described first
The bacterium number of base auxotype bacillus BH21 is more than or equal to 100,000,000 cfu/ grams.
6. microbial manure according to claim 5, it is characterised in that:It includes following active ingredient：Feces of livestock and poultry,
Potato slag, mushroom residue, cultivation bedding and padding and Methylotrophic bacillus BH21.
7. microbial manure according to claim 6, it is characterised in that quality compares feces of livestock and poultry：Potato slag+mushroom residue+cultivation
Bedding and padding=50-60：40-50, potato slag, mushroom residue, cultivation bedding and padding mix according to arbitrary proportion, Methylotrophic bacillus BH21
Bacterium number more than or equal to 100,000,000 cfu/ grams.
8. the preparation method of the microbial manure described in claim 6 or 7, it is characterised in that：The feces of livestock and poultry, potato slag, mushroom
After mushroom slag and cultivation bedding and padding become thoroughly decomposed, Methylotrophic bacillus BH21 is added in less than 45 DEG C, daily turning once, controls heap
Temperature obtains final product the microbial manure below 45 DEG C, after 4-5 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710070295.3A CN106857674B (en) | 2017-02-09 | 2017-02-09 | The application of Methylotrophic bacillus BH21 and its microbial manure and preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710070295.3A CN106857674B (en) | 2017-02-09 | 2017-02-09 | The application of Methylotrophic bacillus BH21 and its microbial manure and preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106857674A true CN106857674A (en) | 2017-06-20 |
| CN106857674B CN106857674B (en) | 2019-02-26 |
Family
ID=59166912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710070295.3A Expired - Fee Related CN106857674B (en) | 2017-02-09 | 2017-02-09 | The application of Methylotrophic bacillus BH21 and its microbial manure and preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106857674B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107118045A (en) * | 2017-07-11 | 2017-09-01 | 烟台三合生物科技有限公司 | A kind of technology for the complex micro organism fungicide that apple cultivation is used |
| CN107324950A (en) * | 2017-07-11 | 2017-11-07 | 烟台三合生物科技有限公司 | A kind of technology for the complex micro organism fungicide that tobacco planting is used |
| CN107964523A (en) * | 2018-01-08 | 2018-04-27 | 河南邑鸿善成生物技术有限公司 | Methylotrophic bacillus and its application |
| CN109762765A (en) * | 2019-01-30 | 2019-05-17 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | A kind of decomposed solid fermentation microbial inoculum and its application in agricultural wastes |
| CN112522164A (en) * | 2020-12-25 | 2021-03-19 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | Forest fruit branch decomposing inoculant and preparation method and application thereof |
| CN114717164A (en) * | 2022-05-19 | 2022-07-08 | 河北省科学院生物研究所 | Bacillus belgii ZLP-101 and application thereof |
Citations (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3377873B2 (en) * | 1994-12-05 | 2003-02-17 | 三井化学株式会社 | How to increase the drought or salt tolerance of plants |
| KR20120002690A (en) * | 2010-07-01 | 2012-01-09 | 충북대학교 산학협력단 | Halophilic bacillus licheniformis strain promoting salt stress tolerance in plant and uses thereof |
| CN102732469A (en) * | 2012-07-12 | 2012-10-17 | 云南农业大学 | Bacillus methylotrophicus and application thereof |
| CN103805534A (en) * | 2013-11-28 | 2014-05-21 | 中国农业科学院农业资源与农业区划研究所 | Bacillus methylotrophicus, microorganism bacterium agents and application of bacillus methylotrophicus and microorganism bacterium agents |
| CN103805535A (en) * | 2013-11-28 | 2014-05-21 | 中国农业科学院农业资源与农业区划研究所 | Isothermal layer bacillus, microbial agent and application of isothermal layer bacillus and microbial agent |
| CN104017750A (en) * | 2014-04-14 | 2014-09-03 | 青岛海大生物集团有限公司 | Salt-resistance Bacillusmethylotrophicus strain LJ and application thereof |
| CN104291974A (en) * | 2014-09-28 | 2015-01-21 | 湖北中化东方肥料有限公司 | Microbial fertilizer capable of enhancing salt tolerance of plants and preparation method of microbial fertilizer |
| CN105263328A (en) * | 2012-08-31 | 2016-01-20 | 拜尔作物科学有限合伙公司 | Method of increasing abiotic stress resistance of a plant |
| EP2986726A1 (en) * | 2013-04-17 | 2016-02-24 | Envera LLC | Novel bacillus strains compositions |
| CN105441366A (en) * | 2015-12-31 | 2016-03-30 | 中国农业大学 | Application of Bacillus methylotrophicus ZBL-1 in control of cotton verticillium wilt |
| CN105524874A (en) * | 2016-02-05 | 2016-04-27 | 中国农业科学院植物保护研究所 | Bacillus methylotrophicus strain NKG-1 and plant growth promoting method |
| CN105543132A (en) * | 2015-12-31 | 2016-05-04 | 河南省农业科学院植物保护研究所 | Bacillus methylotrophicus YB-F7 and application thereof in preventing plant diseases |
| CN105602865A (en) * | 2015-12-23 | 2016-05-25 | 河南广安生物科技股份有限公司 | Bacillus subtilis, fermentation method, powder and application |
| US20160150796A1 (en) * | 2013-06-26 | 2016-06-02 | Symbiota, Inc. | Methods of Use of Seed-Origin Endophyte Populations |
| CN105713858A (en) * | 2016-03-07 | 2016-06-29 | 河北省科学院生物研究所 | Bacillus methylotrophicus for producing various lipopeptide antibiotic substances and application of bacillus methylotrophicus |
| CN105925504A (en) * | 2016-05-20 | 2016-09-07 | 南京林业大学 | Bacillus methylotrophicus ZY3A and application thereof to promotion of leaf lettuce growth |
| CN106085925A (en) * | 2016-08-24 | 2016-11-09 | 新疆山川秀丽生物有限公司 | A kind of salt tolerant antibacterial T5S2 and the application in microbial manure thereof |
-
2017
- 2017-02-09 CN CN201710070295.3A patent/CN106857674B/en not_active Expired - Fee Related
Patent Citations (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3377873B2 (en) * | 1994-12-05 | 2003-02-17 | 三井化学株式会社 | How to increase the drought or salt tolerance of plants |
| KR20120002690A (en) * | 2010-07-01 | 2012-01-09 | 충북대학교 산학협력단 | Halophilic bacillus licheniformis strain promoting salt stress tolerance in plant and uses thereof |
| CN102732469A (en) * | 2012-07-12 | 2012-10-17 | 云南农业大学 | Bacillus methylotrophicus and application thereof |
| CN105263328A (en) * | 2012-08-31 | 2016-01-20 | 拜尔作物科学有限合伙公司 | Method of increasing abiotic stress resistance of a plant |
| US20160312316A1 (en) * | 2013-04-17 | 2016-10-27 | Envera, Llc | Novel bacillus strains and compositions |
| EP2986726A1 (en) * | 2013-04-17 | 2016-02-24 | Envera LLC | Novel bacillus strains compositions |
| US20160150796A1 (en) * | 2013-06-26 | 2016-06-02 | Symbiota, Inc. | Methods of Use of Seed-Origin Endophyte Populations |
| CN103805535A (en) * | 2013-11-28 | 2014-05-21 | 中国农业科学院农业资源与农业区划研究所 | Isothermal layer bacillus, microbial agent and application of isothermal layer bacillus and microbial agent |
| CN103805534A (en) * | 2013-11-28 | 2014-05-21 | 中国农业科学院农业资源与农业区划研究所 | Bacillus methylotrophicus, microorganism bacterium agents and application of bacillus methylotrophicus and microorganism bacterium agents |
| CN104017750A (en) * | 2014-04-14 | 2014-09-03 | 青岛海大生物集团有限公司 | Salt-resistance Bacillusmethylotrophicus strain LJ and application thereof |
| CN104291974A (en) * | 2014-09-28 | 2015-01-21 | 湖北中化东方肥料有限公司 | Microbial fertilizer capable of enhancing salt tolerance of plants and preparation method of microbial fertilizer |
| CN105602865A (en) * | 2015-12-23 | 2016-05-25 | 河南广安生物科技股份有限公司 | Bacillus subtilis, fermentation method, powder and application |
| CN105441366A (en) * | 2015-12-31 | 2016-03-30 | 中国农业大学 | Application of Bacillus methylotrophicus ZBL-1 in control of cotton verticillium wilt |
| CN105543132A (en) * | 2015-12-31 | 2016-05-04 | 河南省农业科学院植物保护研究所 | Bacillus methylotrophicus YB-F7 and application thereof in preventing plant diseases |
| CN105524874A (en) * | 2016-02-05 | 2016-04-27 | 中国农业科学院植物保护研究所 | Bacillus methylotrophicus strain NKG-1 and plant growth promoting method |
| CN105713858A (en) * | 2016-03-07 | 2016-06-29 | 河北省科学院生物研究所 | Bacillus methylotrophicus for producing various lipopeptide antibiotic substances and application of bacillus methylotrophicus |
| CN105925504A (en) * | 2016-05-20 | 2016-09-07 | 南京林业大学 | Bacillus methylotrophicus ZY3A and application thereof to promotion of leaf lettuce growth |
| CN106085925A (en) * | 2016-08-24 | 2016-11-09 | 新疆山川秀丽生物有限公司 | A kind of salt tolerant antibacterial T5S2 and the application in microbial manure thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王洪梅等: "青枯病生防菌N5的特性及其生物学效应", 《土壤》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107118045A (en) * | 2017-07-11 | 2017-09-01 | 烟台三合生物科技有限公司 | A kind of technology for the complex micro organism fungicide that apple cultivation is used |
| CN107324950A (en) * | 2017-07-11 | 2017-11-07 | 烟台三合生物科技有限公司 | A kind of technology for the complex micro organism fungicide that tobacco planting is used |
| CN107964523A (en) * | 2018-01-08 | 2018-04-27 | 河南邑鸿善成生物技术有限公司 | Methylotrophic bacillus and its application |
| CN109762765A (en) * | 2019-01-30 | 2019-05-17 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | A kind of decomposed solid fermentation microbial inoculum and its application in agricultural wastes |
| CN112522164A (en) * | 2020-12-25 | 2021-03-19 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | Forest fruit branch decomposing inoculant and preparation method and application thereof |
| CN112522164B (en) * | 2020-12-25 | 2023-04-21 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | Forest fruit branch decomposition microbial inoculum and preparation method and application thereof |
| CN114717164A (en) * | 2022-05-19 | 2022-07-08 | 河北省科学院生物研究所 | Bacillus belgii ZLP-101 and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106857674B (en) | 2019-02-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2479253B1 (en) | Antagonistic bacteria for preventing and treating panama wilt disease of continuously planted banana and microorganism organic fertilizer thereof | |
| CN106857674B (en) | The application of Methylotrophic bacillus BH21 and its microbial manure and preparation method | |
| CN100579940C (en) | Method for preparing biologic organic fertilizer for preventing and curing eelworm harm and use thereof | |
| CN101875571B (en) | Method for preparing enhanced liquid microbial organic fertilizer | |
| CN103524253B (en) | A kind of seedling medium and preparation method thereof and purposes | |
| CN105218177B (en) | The method for preparing composite microbic bacterial fertilizer using silkworm excrement fermentation | |
| CN106883063A (en) | A kind of biological organic fertilizer and its production technology | |
| CN103168668A (en) | Fruit and vegetable seedling culturing substrate prepared by utilizing agricultural wastes | |
| CN106591193A (en) | Bacillus amyloliquefaciens with broad spectrum growth-promoting and stress-resisting effects | |
| CN103004884B (en) | Watermelon biological seed coating agent and Synthesis and applications thereof | |
| CN102249753A (en) | Method for producing multifunctional bioorganic fertilizer and application of multifunctional bioorganic fertilizer | |
| CN111394281B (en) | Bacillus belgii BV03 microbial agent and preparation method and application thereof | |
| CN106554240A (en) | A kind of crops soil improving agent for one-crop succession and preparation method thereof | |
| CN101473853A (en) | Application of Bacillus cereus Bacillus cereusCMCC63305 in agriculture field | |
| CN107188674B (en) | Solanaceous vegetable seedling culture substrate and preparation method thereof | |
| CN106631546A (en) | Bio-organic fertilizer and preparation method thereof | |
| CN104761385A (en) | Multifunctional microbial organic fertilizer and preparation method thereof | |
| CN105439725A (en) | Paenibacillus polymyxa pesticide-fertilizer for farm onsite fermentation and applications thereof | |
| CN107711891A (en) | A kind of method prevented and treated matrimony vine root rot and extremely set | |
| CN106007824B (en) | Composite bacterial fertilizer and preparation method and application thereof | |
| CN106699423A (en) | Lycium barbarum root rot biocontrol preparation and preparation method thereof | |
| CN101891508B (en) | Living body biological organic fertilizer and preparation method thereof | |
| CN110156511A (en) | A kind of biological organic fertilizer and preparation method thereof based on mineral humus and vegetables residuum trichoderma fermentation material | |
| CN103910545B (en) | Utilize pig manure discarded object to produce the method for Trichoderma harzianum bacterial manure | |
| CN109355197B (en) | Growth-promoting bacterium for promoting growth of saline-alkali soil alfalfa and microbial organic fertilizer thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190226 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |