CN106631546A - Bio-organic fertilizer and preparation method thereof - Google Patents
Bio-organic fertilizer and preparation method thereof Download PDFInfo
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- CN106631546A CN106631546A CN201610812306.6A CN201610812306A CN106631546A CN 106631546 A CN106631546 A CN 106631546A CN 201610812306 A CN201610812306 A CN 201610812306A CN 106631546 A CN106631546 A CN 106631546A
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- organic fertilizer
- lactic acid
- bacillus
- wormwood
- acid bacteria
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses bio-organic fertilizer and a preparation method thereof. The preparation method comprises steps as follows: shiitake chaff and chicken manure are mixed to serve as a main raw material, wormwood lactic acid bacteria are added for anaerobic fermentation deodorization, compound bacilli are added for aerobic high-temperature fermentation and anaerobic post-fermentation, and the bio-organic fertilizer is obtained, wherein the compound bacilli comprise bacillus subtilis and bacillus megaterium. The bio-organic fertilizer prepared with the process is characterized in that the organic content is 38wt% or higher, the content of total nutrients is higher than 5wt%, probiotics are more than 20 million, nutrients are rich, soil properties are improved, the yield of fruit trees or crops is increased, and the quality of the fruit trees or crops is improved. An energy-saving and environment-friendly green production process is fully reflected in the production of the bio-organic fertilizer, and the bio-organic fertilizer is low in preparation cost, high in effective ingredient, good in pathogene inhibiting and disease controlling effects and very significant in economic and social benefit, and conforms to the sustainable development industry policy advocated by the nation.
Description
Technical field
The invention belongs to organic fertilizer production technical field, more particularly to a kind of biological organic fertilizer and preparation method thereof.
Background technology
As country advocates environmental protection, using biological pesticide preventing and treating not as using biological organic fertilizer it is more economical, safer,
More effectively environmental consciousness gradually strengthens.
Biological organic fertilizer can not only play antibacterial, phosphorus decomposing, potassium decomposing, fixed nitrogen etc., also with improving soil quality, disease resistance
By force, the advantages of promoting plant growth, improve Crops production and quality.
The production sweat of biological organic fertilizer is mainly aerobic fermentation and two stages of anaerobic fermentation.
Aerobic fermentation process temperature is high, can reach kill fraction of pathogens bacterium, promote raw material to become thoroughly decomposed and reduce feed moisture content
Deng effect；Anaerobic fermentation mainly experiences acid fermentation process (lactic acid producing, acetic acid or hydrogen process) and alkaline fermentation process (is produced
Ammonia, methane process).Energy decomposing organic matter completely, preserves nitrogen content in product.
The function bacterial classification of biological organic fertilizer is Antagonistic Fungi, mainly some bacillus.Monica L Elliott
(Viability and stability of biological control agents on cotton and snap bean
Seeds.Pest.Manag.SCI., 2001,57 (8):695-706), (bacillus subtilis metabolin Quality Research enters Liu Xue etc.
Exhibition and its application in disease flocking biocontrol, Chinese biological preventing and treating, 2006 (10):179-184), (bacillus subtilis such as Cheng Hongbin
Preventing and treating fungal diseases of plants progress, Shanghai Agricultural journal, 2006,22 (1):109-110) result of the test shows, with fungi,
Microbial inoculum made by non-bacillus is compared, and gemma bacillus agent is more stable, and adaptability is higher, and prevention effect becomes apparent from.And
Add the bacillus for antagonism in biological organic fertilizer to be mainly：Bacillus subtilis (Bacillus subtilis),
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), bacillus megaterium (Bacillus megaterium),
Bacillus licheniformis (Bacillus lincheniformis) etc..
Biological organic fertilizer also can activating soil, promotion plant growth.(the phosphorus decomposing effect of bacillus megaterium JD-2 such as Zhang Weina
Fruit and the research to soil available phosphorus, Jilin agricultural sciences, 2012 (5):38-41) experimental result shows, bacillus megaterium
It is a kind of preferable phosphate solubilizing bacteria, the phosphorus that hardly possible in soil is utilized can be transformed into available phosphorus.
The towering grade of Zhangjiang (progress of lactic acid bacteria antioxidation activity, China Dairy Industry, 2005,33 (1):It is 34-36) total
Knot confirms " lactic acid bacteria has in vivo and in vitro good antioxidation ".And point out that in biological organic fertilizer is developed, addition is appropriate
Lactic acid bacteria can suppress the oxidation of crops, and lactic acid bacteria can also promote farming as a kind of probio, Nantural non-toxic
The growth of thing.Zhang Hongyin etc. (progress that saccharomycete is prevented and treated postharvest disease of fruits and vegetables, EI, 2003,19 (4):
23-25) point out saccharomycete can prevent and treat postharvest fruit and vegetable disease, to this disease have obvious inhibitory action, mechanism be mainly space with
The competition of nutrition, induce parasitic and direct parasitism etc..
The good professor according to husband of the ratio of Japanese university of Ryukyu study successfully EM bacterium (Effective in nineteen eighty-two
Microorganisms).EM bacterium are more than the 80 micro- lifes of 10 category based on photosynthetic bacteria, lactic acid bacteria, saccharomycete and actinomyces
A kind of microorganism live bacteria preparation that thing is composited.The mechanism of action is to form the competition of EM bacterium and pathogenic microorganism contention nutrition,
Due to EM bacterium in soil easily survival and reproduction, so the ecologic niche in soil can be occupied comparatively fast and stably, formation is beneficial
Microbial bacteria advantage group, so as to control the breeding and the invasion and attack to crop of pathogenic microorganism.It is the development of the ecological agriculture
Direction, is more beneficial for the sustainable development of agricultural.
Edible fungus bran is to harvest remaining culture medium waste after product.With edible mushroom popularization, mushroom bran is also into difficulty
With the discarded object for processing.Hou Lijuan etc. (nutritive value of mushroom bran and the application in cultivation, northern gardening, 2008 (7):91-
93) Article analysis point out, water-soluble support containing edible fungi body protein, secondary metabolite, trace element etc. are various in mushroom bran
Divide and abundant organic substance.These materials only have the fermentation that ECDC is managed to decompose, just beneficial to the granule knot kept with culture soil
Structure and physicochemical property, it is also possible to become excellent organic fertilizer.As mushroom mushroom bran contains thick protein 8.76%, crude fibre
30.00%th, crude fat 0.62%, coarse ash 7.93%, calcium 1.08%, phosphorus 0.36%.
Now poultry husbandry is extremely widespread for China, and chicken manure is rich in organic matter, to improving soil texture, increases crop yield,
Improve crop quality obvious to all.Wang Xiaoming (analyze and its develop, hubei agricultural science by chicken manure conventional nutrients
2013,52 (21):5314-5316) Measurement results show, air-dry and contain in layer hen manure crude protein 22.34%, crude fat
2.44%th, crude fibre 10.72%, coarse ash 31.03%, calcium 10.00%, phosphorus 2.36%.Calcium, phosphorus content are higher, especially calcium
It is respectively 31.25 times and 476.19 times of dregs of beans and corn.Li Xiujin (research that chicken manure resource synthetic development is utilized, agriculture work
Journey journal, 1995,11 (1):116-120.) research has shown that：Chicken manure makees feed not as making fertilizer, and as fertilizer, according to formula
The principle of fertilising, for soil type and crop varieties various organic composite fertilizers can be processed into.
The present invention is based on above technology, Mixed Microbes diseases prevention biological organic fertilizer to be researched and developed emphatically, i.e., can anti-bacterial disease, and
Energy restraining epiphyte disease hazard, and different formulations can be prepared according to different soils.
The application of biological organic fertilizer reduces pollution of the organic waste to air, water source and soil, and meeting China can hold
Continuous development strategy.
The content of the invention
It is an object of the invention to provide a kind of biological organic fertilizer with biological disease-preventing effect and preparation method thereof, with breast
Mushroom mushroom bran, chicken manure fermenting into the organic matter for becoming thoroughly decomposed, then are carried out the production of secondary aerobic fermentation there is preventing and treating soil to pass by sour bacteria agent
The fertilizer of disease, and field experiment has been carried out, achieve good effect.The method is mushroom mushroom bran, chicken manure processing and utilization
There is provided an effective way.
In order to realize above-mentioned task, the present invention adopts following technical solution：
A kind of biological organic fertilizer, including mixing as primary raw material using mushroom mushroom bran and chicken manure, is initially charged wormwood lactic acid bacteria
Carry out anaerobic fermentation it is deodorant after, adding composite bacillus carries out obtaining final product biology after aerobic high-heat fermentation and anaerobism afterripening fermentation
Fertilizer；
Described composite bacillus include bacillus subtilis and bacillus megaterium.
Specifically, described wormwood lactic acid bacteria is the lactic acid bacteria that wormwood Jing molasses pickle acquisition.
More specifically, the mass ratio of mushroom mushroom bran and chicken manure is 1:1.2～2, the inoculum concentration of wormwood lactic acid bacteria is 1wt%,
The inoculum concentration of composite bacillus be 0.1wt%, wherein the inoculum concentration of bacillus subtilis be 0.05wt%, bacillus megaterium
Inoculum concentration be 0.05wt%.
In addition, described mushroom mushroom bran by went out mushroom spawn stick crush form, the particle diameter after crushing be 1～
10mm；
By mass percentage, mushroom mushroom bran include thick protein 8.76%, crude fibre 30.00%, crude fat 0.62%,
Ash content 7.93%, calcium 1.08% and phosphorus 0.36%, remaining is moisture, and the total amount of mentioned component is 100%；
By mass percentage, described chicken manure includes thick protein 22.34%, crude fibre 10.72%, crude fat
2.44%th, ash content 31.03%, calcium 10.00% and phosphorus 2.36%, balance of water, the total amount of mentioned component is 100%.
The preparation method of described biological organic fertilizer, the described wormwood lactic acid bacteria that is initially charged carries out the deodorant bag of anaerobic fermentation
Include：Press mushroom mushroom bran：Chicken manure：Wormwood lactic acid bacteria agent=1:2:0.02 mass ratio is well mixed that to carry out anaerobic fermentation deodorant；
Wormwood lactic acid bacteria agent include viable bacteria index be 10～2,000,000,000/ml Lactobacillus casei and viable bacteria index be 0.05～0.2 hundred million/
The alcohol yeast of ml.
Specifically, the preparation of described wormwood lactic acid bacteria agent includes：The lactic acid bacteria that wormwood Jing molasses are pickled into acquisition is former
Plant to be inoculated in the molasses nutrient solution that mass fraction is 10% and cultivate, cultivation temperature is 32 DEG C, to Lactobacillus casei viable bacteria index
For 10～2,000,000,000/ml and alcohol yeast viable bacteria 0.05～0.2 hundred million/ml of index, wormwood lactic acid bacteria agent is obtained final product.
Further, described aerobic high-heat fermentation includes piling up the batch mixing after wormwood lactic acid bacteria anaerobic fermentation is deodorant
The bed of material of 50～70cm of thickness, on the bed of material surface composite bacillus bacterium powder of batch mixing amount 0.1wt% is sprinkled, and mixes and control material thoroughly
Heap temperature is 40～60 DEG C, carries out aerobic high-heat and ferments 7～10 days；The viable bacteria index of composite bacillus bacterium powder be 1～2 ×
109Individual/g.
More specifically, the preparation method of composite bacillus bacterium powder includes：Amplification Culture withered grass is distinguished using conventional method
Bacillus and bacillus megaterium, respectively obtain bacteria containing amount for the bacillus subtilis seed liquor of 10～5,000,000,000/ml and containing bacterium
Measure the bacillus megaterium seed liquor for 10～5,000,000,000/ml；
Bacillus subtilis seed liquor and bacillus megaterium seed are inoculated with respectively by inoculum concentration 5wt% in bran mass
Liquid, low temperature drying is standby after 32 DEG C of culture 48h, and it is 1～2 × 10 that two kinds of drying bacterium powder mixed in equal amounts obtain final product viable bacteria index9Individual/g
Composite bacillus bacterium powder.
In addition, the material after aerobic high-heat ferments is carried out into anaerobism afterripening fermentation, the high 1～3m of stockpile, fermentation time
For 20～50 days.
Further, the crushing material through anaerobism afterripening fermentation is particle diameter<The material of 3mm, before pack material is admixed again
The composite bacillus bacterium powder of quality 0.1wt% obtains final product biological organic fertilizer.
The advantageous effect of the biological organic fertilizer of the present invention is as follows：
1st, rich in active ingredient, bacteriostatic and disease prevention effect is obvious
It is deodorant that the present invention first carries out wormwood lactobacillus-fermented using a small amount of lactic acid bacteria agent in mushroom mushroom bran with chicken manure, presses down
Bacterial disease bacterium breeding processed；Add again bacillus carry out aerobic high-heat fermentation deinsectization disinfectant degradation material, most after Jing anaerobism
Afterripening fermentation, substantially increases the concentration of active ingredient in fertilizer, obvious using actual disease prevention effect.
2nd, soil property is improved, to plant, agricultural product security
There is the phosphorus that a large amount of difficulties are utilized in soil, and plant lacks phosphorus and is easily caused short and small, growth retardation etc..Result of the test shows
Show, composite bacillus group is stronger to the phosphorus dissolving situation of Phos, organophosphor.
Applying pesticide easily pollutes environment with harm people and animals；And applying biological organic fertilizer obviously can avoid the disadvantage of these two aspects
End, can most importantly reduce crop pest dip-dye.In crop growth, plant disease is mostly fungi.And it is most antibacterial
Test result indicate that the thalline of bacillus is preferable to fungi inhibitory action, especially two or more mixing bacillus bacteriostasis rates can
Up to more than 70%.
The approach of bacillus synthetic antimicrobial material mainly has ribosomes approach and non-ribosomal approach, the master that the former synthesizes
If some bacteriocins, chitinase, beta-1,3-glucanase and antibacterial protein etc.；Mainly some volatility of the latter's synthesis
Antibacterial material and lipopeptide antibiotic.The Biocontrol Effect mechanism of bacillus be mainly Competition, inducing plant resistance effect,
Bacteriolysis, antagonism and promotion Effects on Plant Growth etc..These enzymes and antibiotic have no toxic side effect to people, animal, also not
Easily remain in plant body, it is clear that very safe to agricultural product.Raw material sources are wide, low production cost.
3rd, the chicken manure that the present invention is used is the excreta in chicken farm；Mushroom mushroom bran is to plant the discarded object after mushroom；Utilize
This two kinds of material production fertilizers, are all that waste material is recycled, and can reduce environmental pollution, improve social benefit.
Specific embodiment
The biological organic fertilizer of the present invention, including mixing as primary raw material using mushroom mushroom bran and chicken manure, is initially charged wormwood breast
Sour bacterium carry out anaerobic fermentation it is deodorant after, adding composite bacillus carries out being obtained final product after aerobic high-heat fermentation and anaerobism afterripening fermentation
Biological organic fertilizer；Wormwood lactic acid bacteria is the lactic acid bacteria that wormwood Jing molasses pickle acquisition；Composite bacillus include bacillus subtilis
Bacterium and bacillus megaterium.
Mushroom mushroom bran is mixed with chicken manure in the form of powder；
Mushroom mushroom bran of the present invention by went out mushroom spawn stick crush form, the particle diameter after crushing be 1～
10mm, being configured to of bacterium rod is divided into wood chip 48%, wheat bran 11%, gypsum 1% and balance of water, and mentioned component is by mass percentage
Meter, total amount is 100%.Mushroom mushroom bran contains thick protein 8.76%, crude fibre 30.00%, crude fat 0.62%, ash content
7.93%th, calcium 1.08%, phosphorus 0.36%, water 51.25%.According to analysis, mushroom mushroom bran per ton is nitrogenous 10 kilograms, 1 kilogram of phosphorus, potassium 10
Kilogram, and carbon-nitrogen ratio is little, nutrient is in quick-acting states, is easily absorbed and used by plants.
Chicken manure of the present invention is the semi-moist solid manure rushed out from hen house with a small amount of water, is the chicken of common laying hen
Excrement；Contain thick protein 22.34%, crude fibre 10.72%, crude fat 2.44%, ash content 31.03%, calcium in air-dried laying hen chicken manure
10.00%th, phosphorus 2.36%, and 21.11% moisture.
It is by mushroom mushroom bran, chicken manure, wormwood lactic acid bacteria agent in mass ratio 1 that described heap fermentation is deodorant:2:0.02
Ratio is well mixed in chicken manure pond, remove in it is outdoor (outdoor environment affects little for sweat, as long as natural ring
Border all can) pile up carry out fermentative deodorizing within 5～10 days；Described aerobic high-heat fermentation is special deodorant batch mixing immigration is piled up
In fermentation tank, admix the 0.1wt% composite bacillus bacterium powders of batch mixing amount, with mixer periodic agitation carry out aerobic fermentation 7～
10 days, then remove fermentation tank indoors place (covered court does not have excessive requirement for temperature and humidity, as long as rain-proof
Environment) pile up anaerobism afterripening fermentation；Described anaerobism afterripening fermentation refers to the batch mixing of Jing aerobic high-heat fermenting-ripenings, heap
Product carries out anaerobism afterripening fermentation 20～50 days in indoor spacious place, the high 1～3m of stockpile.The fertilizer of fully after-ripening can
Crush, sieve, admix the 0.1wt% composite bacillus bacterium powders of output during packaging again.
Preparation manipulation step is as follows：
(1) prepared by wormwood lactic acid bacteria agent：Wormwood Jing molasses pickle the wormwood lactic acid bacteria original seed of acquisition, weigh wormwood lactic acid
Bacterium original seed 200kg adds the 1000L mass fractions to be in 10% molasses nutrient solution, existing in 32 DEG C or so deep drainpipes 5～7 days
The lactic acid bacteria production kind of activation wormwood.According to production scale prepare appropriate molasses culture medium (molasses containing 3wt%, 0.5wt% salt and
Water 96.5wt%).Adopt the plastic tank of 2000L for container, pour molasses culture medium 1000L for preparing into plastic tank, connect
(i.e. 1000L) activation wormwood lactic acid bacteria production kind of amount and the wormwood lactic acid bacteria original seed of 1wt% are (in order to accelerate to reach the viable bacteria
Bacterium number), cultivate 72h at 32 DEG C and obtain final product wormwood lactic acid bacteria agent.Made by wormwood lactic acid bacteria agent using colony counting method detect
Its viable count, Lactobacillus casei viable bacteria 10～2,000,000,000/ml of index, distillery yeast 0.05～0.2 hundred million/ml, as qualified wormwood breast
Sour bacteria agent.
(2) prepared by composite bacillus bacterium powder：Two kinds of bacillus (hay bacilluses of Amplification Culture are distinguished using conventional method
With huge bacillus) test tube stock, triangular flask liquid original seed be standby；Needed to prepare appropriate solid bran mass (bran according to production
Skin 60wt%, water 40wt%), 121 DEG C of sterilizing 20min are cooled to 30 DEG C or so, spray into the bacillus liquid original seed of 5wt%
(two kinds of bacterium breed respectively), stirs, and low temperature drying is standby after 32 DEG C of culture 48h.Two kinds of drying bacterium powder mixed in equal amounts were both obtained
(viable bacteria index is 1～2 × 10 to composite bacillus bacterium powder9Individual/g).
(3) mushroom mushroom bran mixes deodorant with chicken manure, lactic acid bacteria：By Lenlinus edodes chaff powder, chicken manure (semi-moist solid), wormwood lactic acid
Bacteria agent in mass ratio 1:2:0.02 ratio is well mixed in pond, and being deposited in outdoor rickyard with forklift output carries out anaerobism
Fermentative deodorizing, excessive moisture is back in manure pit and recycles.
(4) batch mixing fermentation bed aerobic high-heat fermentation：Through the deodorant batch mixing of wormwood lactobacillus-fermented, moved into forklift indoor
In fermenting groove, stockpile thickness is 50～70cm.The composite bacillus bacterium of batch mixing amount 0.1wt% is sprinkled on stockpile surface
Powder, is mixed thoroughly with mixer, control stockpile temperature and stirring number of times, is carried out aerobic high-heat and is fermented 7～10 days, then is removed and carried out
Afterripening fermentation.
(5) after-ripening packaging：Material after aerobic high-heat fermentation, with forklift fermentation tank is removed, and is deposited in indoor spaciousness
Place carry out anaerobism after-ripening, the high 1～3m of stockpile, 20～50 days time.Can crush, sieve through the fertilizer of abundant after-ripening
(crossing the mesh screen of 6 mesh), the composite bacillus bacterium powder packaging for admixing output 0.1wt% before pack again is sold.
The plastic tank that wormwood lactic acid bacteria agent production described in step (1) is adopted, cleaning to be sterilized after draining directly add
Molasses culture medium inoculation fermentation；Composite bacillus bacterium powder described in step (2) is sent out using cheap wheat bran solid medium
Ferment is obtained；Mushroom mushroom bran described in step (3) and chicken manure mixed stack mass ratio are an extensive estimative figures, general chicken manure accounting example
Bigger preferably (the mushroom brans of number：Chicken manure=1:1.2～2)；Will be according to temperature of charge in aerobic high-heat sweat described in step (4)
(temperature control of material is 40～60 DEG C) control mixing time number of times；In fertilizer After-ripening described in step (5), if
Material moisture (moisture of control material is less than 35%) bigger than normal, also needs to forklift mobile material to distribute moisture halfway.It is organic
A small amount of composite bacillus bacterium powder is sprinkled into again during fertilizer packaging to mix thoroughly, bag distribution packaging.
The present invention is described in further detail below in conjunction with example, it should be noted that the invention is not restricted to following reality
Apply example.
Composite bacillus bacterium powder and wormwood lactic acid bacteria agent in following embodiments specifically follow these steps to operation：
(1) prepared by composite bacillus bacterium powder：Refrigerator store bacillus subtilis 1 is taken, is respectively transferred 10 in super-clean bench
Bacteria culture media slant tube, 32 DEG C of culture 48h are standby；Prepare 4000mL beef extract-peptone bacterial liquid culture mediums, packing
In the triangular flask of 20 500ml, sealed membrane seals 121 DEG C of sterilizing 30min cooling inoculations, and every test tube stock is inoculated with 5 three
Angle bottle fluid nutrient medium, in shaking table culture 72h of 32 DEG C of 150r/min, it is standby for seed liquor (in seed liquor bacteria containing amount be 10～
5000000000/ml)；Wheat bran 48kg is weighed, the 32kg that adds water stirs, loads and be inoculated with after the 121 DEG C of sterilizing 30min coolings of high temperature resistant bag,
Solid medium in high temperature resistant bag is poured into clean plastic tub, the seed liquor of 5wt% is sprayed into little sprayer, at 32 DEG C
Under the conditions of cultivate 48h after, low temperature drying obtains final product bacillus subtilis bacterium powder under the conditions of being placed on 37 DEG C.Same method carries out huge
The preparation of bacillus bacterium powder.
Mixed in equal amounts bacillus subtilis bacterium powder and bacillus megaterium bacterium powder obtain final product composite bacillus bacterium powder, are combined bud
The viable bacteria index of spore bacillus is 1～2 × 109Individual/g.
(2) prepared by wormwood lactic acid bacteria agent：The lactic acid bacteria original seed 200kg that tarragon acquisition is pickled with molasses is weighed, is added to
1000L mass fractions are in 10% molasses nutrient solution, 1000L wormwood lactic acid to be obtained within 5～7 days in 32 DEG C or so deep drainpipes
Bacterium production kind.Molasses culture medium (molasses containing 3wt%, 0.5wt% salt, water 96.5wt%) 1000L is prepared, loads 2000L's
In plastic tank, the production kind of 1000L wormwoods lactic acid bacteria is added, add the wormwood lactic acid bacteria original seed of 1wt%, at 32 DEG C bar is cultivated
72h is cultivated under part can obtain 2000L wormwood lactic acid bacteria agents (Lactobacillus casei viable bacteria 10～2,000,000,000/ml of index, distillery yeast
0.05～0.2 hundred million/ml).
(3) prepared by biological organic fertilizer：Mushroom mushroom bran is pressed first：Chicken manure：Wormwood lactic acid bacteria agent=1:2:0.02 quality
Than being well mixed in pond, remove that to be deposited in outdoor rickyard anaerobic fermentation deodorant with forklift, allow in excessive moisture backflow manure pit
Recycle；Through the deodorant batch mixing of wormwood lactobacillus-fermented, being moved into indoor fermenting groove with forklift, stockpile thickness is 50～
70cm.The composite bacillus bacterium powder of batch mixing amount 0.1wt% is sprinkled on stockpile surface, is mixed thoroughly with mixer, control stockpile
Temperature and stirring number of times, carry out aerobic high-heat and ferment 7～10 days, then remove and carry out afterripening fermentation；After aerobic high-heat fermentation
Material, remove fermentation tank with forklift, being deposited in the place of indoor spaciousness carries out anaerobism after-ripening, the high 1～3m of stockpile, the time 20～
50 days.Can crush, sieve through the fertilizer of abundant after-ripening, admix the composite bacillus of output 0.1wt% before pack again
Bacterium powder packaging is sold.
Routinely technology activation spreads cultivation into corresponding microbial inoculum and bacterium powder is used for wormwood lactic acid bacteria and composite bacillus, wherein
Viable bacteria index >=1,000,000,000/mL (Lactobacillus casei viable count 10～2,000,000,000/ml, the distillery yeast 0.05 of wormwood lactic acid bacteria agent
～0.2 hundred million/ml), the viable bacteria index of composite bacillus bacterium powder is 1～2 × 109Individual/g.In the composite bacillus of the present invention
Bacillus subtilis strain and bacillus megaterium bacterial classification be by Xibei Univ. of Agricultural ＆ Forest Science ＆ Technology's Life Science College microorganism reality
Test room offer, it would however also be possible to employ the Bacillus subtilis strain being commercially available on market and bacillus megaterium bacterial classification, Dou Nengman
Foot is prepared and required.
The present invention obtains the wormwood of wormwood lactic acid bacteria and picks up from Qinling Mountain Areas, and the natural environment of this area is good, pollution-free source,
Category low altitude area region.Wormwood is common great Ye wormwoods, and acquisition time is 20～30 days before and after the Dragon Boat Festival.
Molasses used in the present invention are that sugarcane or beet are made the byproduct in the process of sugar, usually palm fibre
Black thick liquid.It is all molasses that concentration is squeezed the juice remaining after white sugar is isolated, therefore containing the nutrients enriched than white sugar
Matter.
The following is the specific embodiment that inventor provides.
Embodiment 1：
Preparing wormwood lactic acid bacteria agent according to implementation of the present invention (mainly includes Lactobacillus casei viable bacteria index 10～20
Hundred million/ml, distillery yeast 0.05～0.2 hundred million/ml) 1200L (per L suitable 1kg, similarly hereinafter), composite bacillus bacterium powder (withered grass gemma
Bacillus and bacillus megaterium) 305kg (181kg being needed in production, 124kg is needed during packaging)；Prepare Lenlinus edodes chaff powder
60000kg, semi-moist chicken manure 120m3(per m3Quite 1000kg, similarly hereinafter).
Specifically it follow these steps to operation：
(1)120m3Semi-moist chicken manure enters manure pit, successively pours 1200L wormwood lactic acid bacteria agents, 60000kg Lenlinus edodes chaff powders into
Yu Chizhong, after being stirred with forklift, output is deposited in outdoor rickyard, and to carry out anaerobic fermentation deodorant, allow excessive moisture flow back in
Recycle in manure pit.
(2) outdoor heap fermentation is moved into indoor trough type fermentation pond, windrow thickness 60cm, stockpile surface after 7 days with forklift
181kg composite bacillus bacterium powders are sprinkled, is started mixer and is stirred, subsequently carried out aerobic high-heat for 1 time every stirring in 2 days and send out
Ferment.
Aerobic fermentation terminates after (3) 10 days, and with forklift fermentation tank is removed, and is deposited in the place anaerobism after-ripening of indoor spaciousness,
The stockpile of high 2.5m is piled, after 30 days, crushed, sieved, admix 124kg composite bacillus bacterium powders during packaging again, mix splitting thoroughly
Packaging, sells.
Embodiment 2：
Wormwood lactic acid bacteria agent (mainly Lactobacillus casei) 600L, compound gemma bar are prepared according to implementation of the present invention
Bacterium bacterium powder (bacillus subtilis and bacillus megaterium) bacterium powder 138kg (needs 81kg, needs during finished product packing in production
57kg)；Prepare Lenlinus edodes chaff powder 30000kg, semi-moist chicken manure 50m3。
Specifically it follow these steps to operation：
(1) first 600L wormwood lactic acid bacteria agents are poured into existing 50m3It is then that 30000kg is fragrant in the manure pit of semi-moist chicken manure
Mushroom chaff powder is also poured into pond, and after being stirred with forklift, output is deposited in outdoor rickyard and carries out anaerobic fermentation with deodorant, allows
Excessive moisture flows back and is recycled in manure pit.
(2) outdoor heap fermentation is moved into indoor trough type fermentation pond, windrow thickness about 60cm after 7 days with forklift, and top layer is spread
Even composite bacillus bacterium powder 81kg, starts mixer and stirs, and subsequently carries out aerobic high-heat fermentation 1 time every stirring in 2 days.
Aerobic fermentation terminates after (1) 10 day, is removed with forklift, is deposited in the place of indoor spaciousness and carries out anaerobism after-ripening, heap
The stockpile of Cheng Gaoyue 2.5m, after banking up 30 days, crushes, sieves, and admixes 57kg composite bacillus bacterium powders during packaging again, mixes thoroughly point
Bag packaging, sells.
(3) biological organic fertilizer that the present invention is developed is contrasted with national standard, see the table below 1：
The biological organic fertilizer composition of the present invention of table 1 is contrasted with GB
Note：1. the biological organic fertilizer of embodiment 1 developed as raw material with 1 part of mushroom mushroom bran and 2 parts of chicken manures is represented；2. represent with
1 part of mushroom mushroom bran and the biological organic fertilizer of embodiment 2 that 1.7 parts of chicken manures are that raw material is developed.
Above-mentioned table result explanation：The biological organic fertilizer produced using the inventive method, steady quality, technical indicator is higher than state
Family's standard.Production method of the present invention is relatively easy, and low cost is workable.
Comparative example 1：
Step is with embodiment 1, but it is (outdoor to carry out three fermentations without wormwood lactic acid bacteria agent and composite bacillus powder
Deodorant, aerobic fermentation, anaerobism after-ripening), to crush, sieve, finished product excrement stink is dense.
Comparative example 2：
Step, except for the difference that without wormwood lactic acid bacteria agent, carries out sending out with embodiment 1 only with composite bacillus
Ferment, crushes, sieves.Its result is that production process and finished product excrement stink are denseer.The present embodiment disease resistance is poor compared with embodiment 1.
Comparative example 3：
Step is that wormwood lactic acid bacteria and single bacillus subtilis are fermented with embodiment 1, except for the difference that fermented bacterium,
Crush, sieve.Its result is that the biological organic fertilizer finished product yield-increasing efficiency is low.
Comparative example 4：
, with embodiment 1, except for the difference that the preparation method of lactic acid bacteria agent is different for step.Concrete grammar is first using wormwood breast
Sour bacterium special culture media carries out triangular flask deep drainpipe lactic acid bacteria original seed, then with fermentation tank expanding propagation agent of lactic acid bacteria, entirely
The culture medium of expanding propagation is both needed to autoclave sterilization, and operation is strict complicated, and energy consumption is higher.The present embodiment practical application effect with
Embodiment 1 is close to.
Comparative example 5：
Step except for the difference that replaces wormwood lactic acid bacteria culturers with embodiment 1 with Lactobacillus delbrueckii bacterial classification, and its result is fermentation
Process and finished product excrement stink are denseer, and the biological organic fertilizer finished product yield-increasing efficiency is relatively low.
Comparative example 6：
Step with embodiment 1, except for the difference that with bafillus natto and bacillus licheniformis replace bacillus subtilis and
Bacillus megaterium, its result is that the biological organic fertilizer finished product yield-increasing efficiency is relatively low.
The practical application effect contrast such as table 2 below of above example 1～2 and comparative example 1～6.
The practical application effect of the embodiment of the present invention of table 2 and comparative example
Note：It is actinidia tree for No. a kind of examination, No. 3 kinds are vine, and soil is moisture soil.It is apple for No. 2 kinds of examination
Tree, Protectorate cultivation.All of fertilizer is applied after fruit picking to before falling leaves, and application process is spreaded manuer in holes compared with many places in root system.Apply
Consumption：No. a kind, every plant of 8kg of No. 2 kinds, every plant of 5kg of No. 3 kinds.
Claims (10)
1. a kind of biological organic fertilizer, it is characterised in that include using mushroom mushroom bran and chicken manure mixing as primary raw material, be initially charged Chinese mugwort
Careless lactic acid bacteria carry out anaerobic fermentation it is deodorant after, adding composite bacillus is carried out after aerobic high-heat fermentation and anaerobism afterripening fermentation
Obtain final product biological organic fertilizer；
Described composite bacillus include bacillus subtilis and bacillus megaterium.
2. biological organic fertilizer as claimed in claim 1, it is characterised in that described wormwood lactic acid bacteria is that wormwood Jing molasses are pickled
The lactic acid bacteria of acquisition.
3. biological organic fertilizer as claimed in claim 1 or 2, it is characterised in that the mass ratio of mushroom mushroom bran and chicken manure is 1:1.2
～2, the inoculum concentration of wormwood lactic acid bacteria is 1wt%, and the inoculum concentration of composite bacillus is 0.1wt%, wherein bacillus subtilis
Inoculum concentration be 0.05wt%, the inoculum concentration of bacillus megaterium is 0.05wt%.
4. biological organic fertilizer as claimed in claim 1 or 2, it is characterised in that described mushroom mushroom bran is by going out mushroom
What bacterium rod was crushed, the particle diameter after crushing is 1～10mm；
By mass percentage, mushroom mushroom bran includes thick protein 8.76%, crude fibre 30.00%, crude fat 0.62%, ash content
7.93%th, calcium 1.08% and phosphorus 0.36%, remaining is moisture, and the total amount of mentioned component is 100%；
By mass percentage, described chicken manure include thick protein 22.34%, crude fat 2.44%, crude fibre 10.72%,
Ash content 31.03%, calcium 10.00% and phosphorus 2.36%, balance of water, the total amount of mentioned component is 100%.
5. the preparation method of the biological organic fertilizer described in claim 1,2,3 or 4, it is characterised in that described is initially charged wormwood
Lactic acid bacteria carry out anaerobic fermentation it is deodorant including：Press mushroom mushroom bran：Chicken manure：Wormwood lactic acid bacteria agent=1:2:0.02 mass ratio is mixed
It is deodorant that conjunction uniformly carries out anaerobic fermentation；Wormwood lactic acid bacteria agent includes the Lactobacillus casei that viable bacteria index is 10～2,000,000,000/ml
With the alcohol yeast that viable bacteria index is 0.05～0.2 hundred million/ml.
6. the preparation method of biological organic fertilizer as claimed in claim 5, it is characterised in that described wormwood lactic acid bacteria agent
Preparation includes：The lactic acid bacteria original seed that wormwood Jing molasses pickle acquisition is inoculated in the molasses nutrient solution that mass fraction is 10% and is trained
Support, cultivation temperature is 32 DEG C, to Lactobacillus casei viable bacteria index be 10～2,000,000,000/ml and alcohol yeast viable bacteria index is 0.05
～0.2 hundred million/ml, obtains final product wormwood lactic acid bacteria agent.
7. the preparation method of biological organic fertilizer as claimed in claim 5, it is characterised in that described aerobic high-heat fermentation includes
Batch mixing after wormwood lactic acid bacteria anaerobic fermentation is deodorant is piled up into the bed of material for 50～70cm of thickness, is sprinkled on bed of material surface mixed
The composite bacillus bacterium powder of doses 0.1wt%, mixes and controls stockpile temperature thoroughly for 40～60 DEG C, carries out aerobic high-heat fermentation 7
～10 days；The viable bacteria index of composite bacillus bacterium powder is 1～2 × 109Individual/g.
8. the preparation method of biological organic fertilizer as claimed in claim 7, it is characterised in that the preparation of composite bacillus bacterium powder
Method includes：Amplification Culture bacillus subtilis and bacillus megaterium are distinguished using conventional method, respectively obtaining bacteria containing amount is
The bacillus subtilis seed liquor of 10～5,000,000,000/ml and the bacillus megaterium seed liquor that bacteria containing amount is 10～5,000,000,000/ml；
Bacillus subtilis seed liquor and bacillus megaterium seed liquor are inoculated with respectively by inoculum concentration 5wt% in bran mass,
32 DEG C of culture 48h post-dryings are standby, and it is 1～2 × 10 that two kinds of drying bacterium powder mixed in equal amounts obtain final product viable bacteria index9The compound bud of individual/g
Spore bacillus bacterium powder.
9. the preparation method of biological organic fertilizer as claimed in claim 7, it is characterised in that by after aerobic high-heat ferments
Material carries out anaerobism afterripening fermentation, the high 1～3m of stockpile, and fermentation time is 20～50 days.
10. the preparation method of biological organic fertilizer as claimed in claim 9, it is characterised in that through the thing of anaerobism afterripening fermentation
Feed powder is broken for particle diameter<The material of 3mm, the composite bacillus bacterium powder for admixing quality of material 0.1wt% before pack again obtains final product biology
Fertilizer.
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| CN107141029A (en) * | 2017-05-26 | 2017-09-08 | 江西禾旺生物科技有限公司 | A kind of fermented organic fertilizer and preparation method thereof |
| CN108112541A (en) * | 2017-12-26 | 2018-06-05 | 杨凌欣益生态农业科技发展有限公司 | A kind of cultural method of livestock and poultry cultivation zero-emission |
| CN108440060A (en) * | 2018-05-02 | 2018-08-24 | 辽宁省农业科学院 | A kind of method and application preparing the microbial-bacterial fertilizer for promoting hickory chick to grow using mushroom mushroom bran |
| CN108586148A (en) * | 2018-06-25 | 2018-09-28 | 天柱县森源米业有限责任公司 | A kind of Rice Cropping dedicated fertilizer |
| CN108676746A (en) * | 2018-05-22 | 2018-10-19 | 福建省农业科学院农业生物资源研究所 | A kind of preparation method and product for integrating microorganism group microbial inoculum |
| CN110240527A (en) * | 2019-07-19 | 2019-09-17 | 海南北纬十八度果业有限公司 | A kind of bacteria agent and preparation method thereof and biological bacterium organic fertilizer and preparation method thereof |
| CN110590468A (en) * | 2019-11-07 | 2019-12-20 | 李光学 | Method for preparing organic fertilizer by using sugar refinery filter mud |
| CN111978120A (en) * | 2020-08-25 | 2020-11-24 | 湖南启农生物科技有限公司 | Liquid environment-friendly composite microbial fertilizer and preparation method thereof |
| CN112479790A (en) * | 2020-12-31 | 2021-03-12 | 河南比赛尔农业科技有限公司 | Novel mushroom fungus chaff fertilizer |
| CN114213160A (en) * | 2021-12-29 | 2022-03-22 | 刘西莉 | Process for producing composite biological bacterial fertilizer from livestock and poultry manure |
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| CN108440060A (en) * | 2018-05-02 | 2018-08-24 | 辽宁省农业科学院 | A kind of method and application preparing the microbial-bacterial fertilizer for promoting hickory chick to grow using mushroom mushroom bran |
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| CN110590468A (en) * | 2019-11-07 | 2019-12-20 | 李光学 | Method for preparing organic fertilizer by using sugar refinery filter mud |
| CN111978120A (en) * | 2020-08-25 | 2020-11-24 | 湖南启农生物科技有限公司 | Liquid environment-friendly composite microbial fertilizer and preparation method thereof |
| CN112479790A (en) * | 2020-12-31 | 2021-03-12 | 河南比赛尔农业科技有限公司 | Novel mushroom fungus chaff fertilizer |
| CN114213160A (en) * | 2021-12-29 | 2022-03-22 | 刘西莉 | Process for producing composite biological bacterial fertilizer from livestock and poultry manure |
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Inventor after: Chen Deyu Inventor after: Yu Huiqin Inventor after: Tian Guangwen Inventor after: Yuan Bing Inventor before: Chen Deyu Inventor before: Jin Dongliang Inventor before: Tian Guangwen |
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Application publication date: 20170510 |