CN104761385A - Multifunctional microbial organic fertilizer and preparation method thereof - Google Patents

Multifunctional microbial organic fertilizer and preparation method thereof Download PDF

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Publication number
CN104761385A
CN104761385A CN201510168314.7A CN201510168314A CN104761385A CN 104761385 A CN104761385 A CN 104761385A CN 201510168314 A CN201510168314 A CN 201510168314A CN 104761385 A CN104761385 A CN 104761385A
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China
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fertilizer
straw
multifunctional microbial
fermentation
agent
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刘铜
荆晶
侯巨梅
左豫虎
张有利
靳亚忠
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Heilongjiang Bayi Agricultural University
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Heilongjiang Bayi Agricultural University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention relates to a multifunctional microbial organic fertilizer and a preparation method thereof. The multifunctional microbial organic fertilizer is prepared by mixing a fermented agricultural organic fertilizer with a multi-effect biological fertilizer provided by the Chinese patent ZL201210108437.8, wherein the weight percent of the fermented agricultural organic fertilizer is 70%-90%; and the weight percent of the multi-effect biological fertilizer is 10%-30%; the fermented agricultural organic fertilizer is an organic fertilizer which is produced by mixing a facultative anaerobic bacteria flora seed leavening agent with agricultural organic waste raw materials in a manner of compost anaerobic fermentation; and the agricultural organic waste raw materials are prepared from any one or a mixture of more of a maize straw, a soybean straw, a straw, weeds and livestock manures. The multifunctional microbial organic fertilizer is complete in nutrient, loose and free of stink; plant diseases and insect pests can be prevented; and the multifunctional microbial organic fertilizer has relatively good prevention and treatment effects on soil-borne diseases.

Description

A kind of multifunctional microbial fertilizer and preparation method thereof
One, technical field
What the present invention relates to is the bio-feritlizer used in growing process, and what be specifically related to is a kind of multifunctional microbial fertilizer and preparation method thereof.
Two, background technology
Due to the excessive exploitation of China's agricultural, partially execute chemical fertilizer of simple substance especially for a long time, unsuitable organic to the supplementary agricultural of soil, cause soil quality to decline, available agricultural resource is progressively exhausted, and Soil degradation is serious, finally affects the production of high quality agricultural products.The current soil resource present situation of China, in the urgent need to organic for measure supplements soil agricultural by people, guarantees agricultural planting level and crop products quality.Corresponding with this situation, the research and development of China's organic fertilizer technique with promote relative backwardness.On market alternative product and organic fertilizer production producer very few especially.Such as, Heilongjiang Province is the main province of China's rice exports.For many years, province grain-export department runs into the problem of having a headache most and is exactly, and usually has the rice exported in batch to be returned by external quality inspection organization.Problem is because the use of long-term a large amount of chemical fertilizer, agricultural chemicals in process of production, makes the quality of rice can not reach the requirement of importer.In addition owing to increasing along with the proportion of crop planting time limit, cause have accumulated a large amount of harmful organisms in soil, disease occurs more and more heavier, and hardening in a large number appears in soil, and fertility and cultivated power decline, and finally causes crop yield, quality significantly declines.Therefore soil fertility decline and soil-borne disease have become the important limiting factor affecting production estimation and quality.Along with people's living standard progressively improves the upgrading with consumption idea, to the lifting of green product demand and country to energy-saving and cost-reducing requirement, the microbial organic fertilizer of development environmental protection becomes current social Developing mainstream.But the fermentation major part of microbial organic fertilizer adopts aerobic fermentation at present, need constantly to stir logical oxygen to compost while fermentation, stench is during the fermentation caused to leak, serious environment pollution and operator healthy, and add equipment cost, energy cost, human cost.In sum, in research process of the present invention, contriver finds that producing microbial organic fertilizer at present exists following problem:
1. the fermentation major part of microbial organic fertilizer adopts aerobic microbiological at present, therefore needs to carry out ventilation oxygen supply at fermenting process, needs to carry out repeatedly turning and stirs operation, and can stench be produced in operation, there is sewage crossing current, cause the waste of the energy, improve equipment cost;
2. the microbial organic fertilizer produced often has certain improvement result to soil fertility and Soil structure, but poor to disease-controlling effect, does not particularly have prevention and control capability to soil-borne disease.
Three, summary of the invention
The object of this invention is to provide a kind of multifunctional microbial fertilizer, this multifunctional microbial fertilizer leaks for solving in prior art stench in microbial organic fertilizer fermenting process, serious environment pollution and the poor problem of disease-controlling effect, another object of the present invention is to provide the preparation method of this multifunctional microbial fertilizer.
The technical solution used in the present invention is: the multieffect biochemical fertilizer that this multifunctional microbial fertilizer is provided by ferment agriculture fertilizer and Chinese patent ZL201210108437.8 is mixed to form, above-mentioned two kinds of components by mass percentage, ferment agriculture fertilizer 70-90%, multieffect biochemical fertilizer 10-30%; The agriculture fertilizer that ferments is the fertilizer utilizing compost anaerobically fermenting mode to produce after using the agent of amphimicrobian flora seed fermentation to mix with agricultural organic waste raw material, agricultural organic waste raw material by corn stalk, soybean straws, straw, weeds, livestocks faces any one form or to be severally mixed to form arbitrarily wherein arbitrarily;
The agent of amphimicrobian flora seed fermentation is prepared as follows :
1) female flora preparation:
Single bacterium colony actinomycetes, yeast, streptomycete are inoculated on yeast solid medicinal extract LB substratum respectively, at 25 DEG C of temperature, cultivate 1-3 days, with aseptic bud label picking list bacterium colony actinomycetes, yeast, streptomycete respectively, then be inoculated in yeast extract LB liquid nutrient medium respectively, 180-220 rev/min, leavening temperature 25-28 DEG C, fermentation time is 2-6 days, then by the single bacterium colony actinomycetes after fermentation, yeast, streptomycete, in single bacterium colony actinomycetes: yeast: the ratio of streptomycete=3:3:4 mixes, mixed solution is female flora;
2) preparation of compost flora seed fermentation agent:
1. female flora stoste step 1) prepared by volume 1:150-200 joins in sterile liquid yeast extract substratum, culture temperature 40-50 DEG C, 180rpm shaking culture 24h;
2. dry rice straw stalk is cut into about 3cm long, the dry rice straw cut short is mixed than 10:4 by mass/volume with water, vibration mixing, 121 DEG C, 20min sterilizing, must soak straw;
3. get above-mentioned steps nutrient solution 1. by volume 1:400-800 add in sterilized water, above-mentioned steps after 1:10-20 joins sterilizing by volume after shaking up 2. soak in straw, vibration stirs, and cultivates 7-10 days, i.e. amphimicrobian flora seed fermentation agent for 50 DEG C.
The preparation method of above-mentioned multifunctional microbial fertilizer:
One, the agent of amphimicrobian flora seed fermentation is prepared;
Two, agricultural organic waste raw material is prepared;
Three, the agriculture fertilizer of compost preparation fermentation:
1. measure floor area, at least ensure that the waste materials heap of 45 cubes is high more than 1.5 meters, initial setting floor area is 15 square metres, spreads thin film in the above, on film, then spread the agent of skim amphimicrobian flora seed fermentation;
2. at the agricultural organic waste raw material of the thick about 30-40cm in amphimicrobian flora seed fermentation agent upper berth, and add water and fully mix thoroughly, make water content be 55-65%, then spread the agent of one deck flora seed fermentation thereon, by that analogy, until stockpile is high more than 1.5 meters;
3. in stockpile, cover one deck waterproof cloth and compacting, measure the temperature in 4 orientation, the four corners of the world with thermometer and note down;
4., until raise waterproof cloth after compost 50-90 days, namely obtain by mixing the agriculture fertilizer that ferments;
Four, the multieffect biochemical fertilizer mixing that fermentation agricultural fertilizer step 3 obtained and Chinese patent ZL201210108437.8 provide, during mixing by mass percentage, ferment agriculture fertilizer 70-90%, multieffect biochemical fertilizer 10-30%, namely obtains multifunctional microbial fertilizer.
In such scheme, multifunctional microbial fertilizer 1-50 kilogram of plastic packaging bag is packed.
In such scheme, agricultural organic waste raw material is mixed to form by corn stalk 50% by mass percentage and cow dung 50%.
In such scheme, agricultural organic waste raw material is mixed to form by straw straw 50% by mass percentage and cow dung 50%.
In such scheme, agricultural organic waste raw material is mixed to form by straw straw 70 by mass percentage, weeds 10%, cow dung 20%.
Beneficial effect:
1, nutrient is comprehensive:
Fertilizer is a kind of complete manure, containing the large trace element such as moderate-element and a certain amount of iron, copper, zinc, manganese, boron, molybdenum such as abundant carbon, nitrogen, phosphorus, potassium, calcium, magnesium, sulphur, silicon etc. required for crop growthing development.
2, soil is improved:
Fertilizer contains a large amount of organic matter, and long-term application can improve the soil organism, improves soil aggregate, promotes soil microorganisms breeding, makes loosing soil, ventilation, be easy to draining, farming.
3, fertilizer conservation water conservation:
The fertilizer conservation performance that application of organic fertilizers can strengthen soil is eased up impulse force.Soil ulmin is organic colloid, though particle is little, surface-area is large, absorbs large more than 100 times of moisture energy force rate mineral colloid, large 4 times of replacement amount.Long-term application fertilizer, can make organic, increase without volume recombination colloid, soil merges closely, strengthens water conservation, fertilizer conservation, feeder capability.
4, prevention and elimination of disease and pests:
Fertilizer is in fermentation maturity process, the spore and propagulum etc. of a lot of weed seed, insect egg, pathogenic bacterium are killed, and after mixing with a kind of multieffect biochemical fertilizer, when the later stage uses, its microbial strains again can be fermented and be produced a large amount of Bio-control Trichoderma, therefore the generation of disease and pest time on crop, can be prevented to a certain extent, and there is good preventive and therapeutic effect to soil-borne disease.
5, quality of agricultural product is improved:
In view of this product nutrient is complete, loose ordorless, therefore have a lot of application.A lot of city resident is utilizing the family plot limited in family to develop some growing vegetables after work as far as possible now, such as summer is with some mater convolvulus of foam box kind, three-coloured amaranth, then coriander or Chinese cabbage is just changed into winter, the tendril class vegetables also costing some sponge gourd cucumber and so in shelf kind had, when flourishing to midsummer, also constitute the view of one uniqueness.But along with development and the growth in the living standard of science and technology, the shortcoming of this balcony planting more and more highlights.To be exactly the most significantly most resident be all uses and makes compost that a class is dirty and with the fertilizer of strong peculiar smell, those being liked to the resident of indoor cultivation, is definitely worthless.But the fertilizer produced with this fermentation process but can effectively avoid this problem.First be destitute of smell, be secondly that this fertilizer is fully become thoroughly decomposed, organic matter is well decomposed, and nutrient is complete, is conducive to crop and absorbs fast; Beneficial microorganism in fertilizer can also improve soil regime, prevents the generation of a lot of crop pest, reduces the use of agricultural chemicals, makes the vegetable melon and fruit of plantation more healthy.
Not only be widely used on family planting, on all the other crops, the effect of this fertilizer is also fine.When being applied on flowers, make flowers branch stem healthy and strong, bright-colored; Be applied on melon-fruit-like vegetable and fruit tree and fully can improve fruit-setting rate, fruit type is good, and head is large, and sugariness is high, effectively improves per mu yield; Be applied on leaf vegetables, make its blade loose, emerald green, significantly improve output, fresh and tender, the delicious flavour of vegetables, nutritious simultaneously; Be applied on rhizome vegetable, make rhizome very large, improve volume production, effectively prevent the generation of soil-borne disease simultaneously, reduce Pesticide use.
Four, embodiment
The present invention is described further below:
embodiment 1
The multieffect biochemical fertilizer that this multifunctional microbial fertilizer is provided by ferment agriculture fertilizer and Chinese patent ZL201210108437.8 is mixed to form, and by mass percentage, ferment above-mentioned two kinds of components agriculture fertilizer 70%, multieffect biochemical fertilizer 30%; The agriculture fertilizer that ferments is the fertilizer utilizing compost anaerobically fermenting mode to produce after using the agent of amphimicrobian flora seed fermentation to mix with agricultural organic waste raw material, and agricultural organic waste raw material is mixed to form by corn stalk and cow dung.
one, the preparation of amphimicrobian flora seed fermentation agent
1) female flora preparation
By actinomycetes, yeast, streptomycete is inoculated on yeast solid medicinal extract (LB) substratum respectively, at 25 DEG C of temperature, cultivate 1-3 days, with aseptic bud label picking list bacterium colony actinomycetes respectively, yeast, streptomycete, is then inoculated in yeast extract (LB) liquid nutrient medium triangular flask respectively, 180 revs/min, leavening temperature 25 DEG C, fermentation time is 3 days, and be then actinomycetes in proportion: yeast: streptomycete=3:3:4 mixes, mixed solution is female flora;
2) preparation of amphimicrobian flora seed fermentation agent in compost
1. by above-mentioned female flora stoste by volume (v/v) 1:150 join sterile liquid yeast extract substratum, culture temperature 40 DEG C, 180rpm shaking culture 24h.
2. dry rice straw stalk is cut into about 3cm long, the dry rice straw cut short is mixed by mass/volume ratio (g/ml) 10:4 with water, vibration mixing, 121 DEG C, 20min sterilizing, must soak straw.
3. the nutrient solution getting above-mentioned 1. step by volume (v/v) 1:400 adds in sterilized water, the above-mentioned 2. step after (v/v) 1:10 joins sterilizing by volume after shaking up soak in straw, vibration stirs, and cultivates 7 days for 50 DEG C.Namely the agent of amphimicrobian flora seed fermentation is obtained.
Two, agricultural organic waste raw material combines by following per-cent:
Corn stalk 50% and cow dung 50%.
three, compost operating process is as follows:
1. measure floor area, at least ensure that the waste materials heap of 45 cubes is high more than 1.5 meters.Initial setting floor area is 15 square metres, spreads thin film in the above, on film, then spread the agent of skim flora seed fermentation.
2. at the waste materials of the thick about 30cm in flora seed fermentation agent upper berth, and add water and fully mix thoroughly, make water content be 60%, then spread the agent of one deck flora seed fermentation thereon.By that analogy, until stockpile is high more than 1.5 meters.
3. in stockpile, cover one deck waterproof cloth and compacting, measure the temperature in 4 orientation, the four corners of the world with thermometer and note down.
4. until compost raised waterproof cloth after 50 days, the fermentation agricultural fertilizer produced by mixing and composting process.
four, patent of invention (ZL201210108437.8) is shown in above-mentioned a kind of multieffect biochemical fertilizer preparation.
five, functional microorganism fertilizer is prepared:
The fermentation agricultural fertilizer produced by above-mentioned compost mixes with a kind of multieffect biochemical fertilizer, during mixing by mass percentage, and organism of fermentation fertilizer 70%, multieffect biochemical fertilizer 30%.By the mixture that obtains and multifunctional microbial fertilizer, by 50 kilograms of plastic packaging bags packagings.
Can find use present case product in muskmelon, beet and cucumber soil after, seedling stage can promote growing of seedling, makes seedling rhizome sturdy, leaf look dark green, grows healthy and strong, and plant height is apparently higher than common fertilizer, diseases and insect pests resistance is strong, has certain preventive and therapeutic effect especially to cucumber fusarium axysporum.Fruiting period fruit is many, and volume is large, and sugariness is high, and mouthfeel is good, obviously improves achievement quality.
embodiment 2
The multieffect biochemical fertilizer that this multifunctional microbial fertilizer is provided by ferment agriculture fertilizer and Chinese patent ZL201210108437.8 is mixed to form, and by mass percentage, ferment above-mentioned two kinds of components agriculture fertilizer 80%, multieffect biochemical fertilizer 20%; The agriculture fertilizer that ferments is the fertilizer utilizing compost anaerobically fermenting mode to produce after using the agent of amphimicrobian flora seed fermentation to mix with agricultural organic waste raw material, and agricultural organic waste raw material is mixed to form by straw straw and cow dung.
one, the preparation of amphimicrobian flora seed fermentation agent
1) female flora preparation
By actinomycetes, yeast, streptomycete is inoculated on yeast solid medicinal extract (LB) substratum respectively, at 25 DEG C of temperature, cultivate 1-3 days, with aseptic bud label picking list bacterium colony actinomycetes respectively, yeast, streptomycete, is then inoculated in yeast extract (LB) liquid nutrient medium triangular flask respectively, 180 revs/min, leavening temperature 25 DEG C, fermentation time is 3 days, and be then actinomycetes in proportion: yeast: streptomycete=3:3:4 mixes, mixed solution is female flora;
2) preparation of compost amphimicrobian flora seed fermentation agent
1. by above-mentioned female flora stoste by volume (v/v) 1:150 join sterile liquid yeast extract substratum, culture temperature 40 DEG C, 180rpm shaking culture 24h.
2. dry rice straw stalk is cut into about 3cm long, the dry rice straw cut short is mixed by mass/volume ratio (g/ml) 10:4 with water, vibration mixing, 121 DEG C, 20min sterilizing, must soak straw.
3. the nutrient solution getting above-mentioned 1. step by volume (v/v) 1:400 adds in sterilized water, the above-mentioned 2. step after (v/v) 1:10 joins sterilizing by volume after shaking up soak in straw, vibration stirs, and cultivates 7 days for 50 DEG C.Namely the agent of amphimicrobian flora seed fermentation is obtained.
two, agricultural organic waste raw material combines by following per-cent:
Straw straw 50% and cow dung 50%.
three, compost operating process is as follows:
1. measure floor area, at least ensure that the waste materials heap of 45 cubes is high more than 1.5 meters.Initial setting floor area is 15 square metres, spreads thin film in the above, on film, then spread the agent of skim flora seed fermentation.
2. at the waste materials of the thick about 30cm in flora seed bacteria group culture base fluid upper berth, and add water and fully mix thoroughly, make water content be 60%, then spread the agent of one deck flora seed fermentation thereon.By that analogy, until stockpile is high more than 1.5 meters.
3. in stockpile, cover one deck waterproof cloth and compacting, measure the temperature in 4 orientation, the four corners of the world with thermometer and note down.
4. until compost raised waterproof cloth after 60 days, the fermentation agricultural fertilizer produced by mixing and composting process.
four, above-mentioned a kind of multieffect biochemical fertilizer preparation is with reference to patent of invention (ZL201210108437.8)
five, functional microorganism fertilizer is prepared:
The fermentation agricultural fertilizer produced by above-mentioned compost mixes with a kind of multieffect biochemical fertilizer, during mixing by mass percentage, and fermentation fertilizer 80%, multieffect biochemical fertilizer 20%.By the mixture that obtains and multifunctional microbial fertilizer, by 50 kilograms of plastic packaging bags packagings.
In peach plantation after present case multifunctional microbial fertilizer, flourishing, quantity of blooming showed increased, pattern is bright-coloured.Honey peach quantity of bearing fruit is many, and disease occurs few, and sugariness is high, bright in colour, quality better; Do not use the peach of this product, bloom less, leaf is not dark green yet, and peach hip number is less, and serious leaf curl of peach occurs, and cause leaf rolling, Yield and qualities all declines.
embodiment 3
The multieffect biochemical fertilizer that this multifunctional microbial fertilizer is provided by ferment agriculture fertilizer and Chinese patent ZL201210108437.8 is mixed to form, and by mass percentage, ferment above-mentioned two kinds of components agriculture fertilizer 90%, multieffect biochemical fertilizer 10%; The agriculture fertilizer that ferments is the fertilizer utilizing compost anaerobically fermenting mode to produce after using the agent of amphimicrobian flora seed fermentation to mix with agricultural organic waste raw material, and agricultural organic waste raw material is mixed to form by straw straw, weeds, cow dung.
one, the preparation of amphimicrobian flora seed fermentation agent
1) female flora preparation
By actinomycetes, yeast, streptomycete is inoculated on yeast solid medicinal extract (LB) substratum respectively, at 25 DEG C of temperature, cultivate 1-3 days, with aseptic bud label picking list bacterium colony actinomycetes respectively, yeast, streptomycete, is then inoculated in yeast extract (LB) liquid nutrient medium triangular flask respectively, 180 revs/min, leavening temperature 25 DEG C, fermentation time is 3 days, and be then actinomycetes in proportion: yeast: streptomycete C=3:3:4 mixes, mixed solution is female flora;
2) preparation of amphimicrobian flora seed fermentation agent in compost
1. by above-mentioned female flora stoste by volume (v/v) 1:150 join sterile liquid yeast extract substratum, culture temperature 40 DEG C, 180rpm shaking culture 24h.
2. dry rice straw stalk is cut into about 3cm long, the dry rice straw cut short is mixed by mass/volume ratio (g/ml) 10:4 with water, vibration mixing, 121 DEG C, 20min sterilizing, must soak straw.
3. the nutrient solution getting above-mentioned 1. step by volume (v/v) 1:400 adds in sterilized water, the above-mentioned 2. step after (v/v) 1:10 joins sterilizing by volume after shaking up soak in straw, vibration stirs, and cultivates 7 days for 50 DEG C.Namely the agent of amphimicrobian flora seed fermentation is obtained.
Two, agricultural organic waste raw material combines by following per-cent:
Straw straw 70, weeds 10% and cow dung 20%.
three, compost operating process is as follows:
1. measure floor area, at least ensure that the waste materials heap of 45 cubes is high more than 1.5 meters.Initial setting floor area is 15 square metres, spreads thin film in the above, on film, then spread the agent of skim flora seed fermentation.
2. at the waste materials of the thick about 30cm in flora seed fermentation agent upper berth, and add water and fully mix thoroughly, make water content be 60%, then spread the agent of one deck flora seed fermentation thereon.By that analogy, until stockpile is high more than 1.5 meters.
3. in stockpile, cover one deck waterproof cloth and compacting, measure the temperature in 4 orientation, the four corners of the world with thermometer and note down.
4. until compost raised waterproof cloth after 60 days, the fermentation agricultural fertilizer produced by mixing and composting process.
four, patent of invention (ZL201210108437.8) is shown in above-mentioned a kind of multieffect biochemical fertilizer preparation
five, functional microorganism fertilizer is prepared:
The fermentation agricultural fertilizer produced by above-mentioned compost mixes with a kind of multieffect biochemical fertilizer, during mixing by mass percentage, and fermentation fertilizer 90%, multieffect biochemical fertilizer 10%.By the mixture that obtains and multifunctional microbial fertilizer, by 50 kilograms of plastic packaging bags packagings.
After planting lawn district uses present case product, turfgrass side root is many, and root growth is luxuriant; Over-ground part blade quantity is many, and leaf look dense, green in color, resists and tramples.

Claims (6)

1. a multifunctional microbial fertilizer, it is characterized in that: the multieffect biochemical fertilizer that this multifunctional microbial fertilizer is provided by ferment agriculture fertilizer and Chinese patent ZL201210108437.8 is mixed to form, above-mentioned two kinds of components by mass percentage, ferment agriculture fertilizer 70-90%, multieffect biochemical fertilizer 10-30%; The agriculture fertilizer that ferments is the fertilizer utilizing compost anaerobically fermenting mode to produce after using the agent of amphimicrobian flora seed fermentation to mix with agricultural organic waste raw material, agricultural organic waste raw material by corn stalk, soybean straws, straw, weeds, livestocks faces any one form or to be severally mixed to form arbitrarily wherein arbitrarily;
The agent of amphimicrobian flora seed fermentation is prepared as follows :
1) female flora preparation:
Single bacterium colony actinomycetes, yeast, streptomycete are inoculated on yeast solid medicinal extract LB substratum respectively, at 25 DEG C of temperature, cultivate 1-3 days, with aseptic bud label picking list bacterium colony actinomycetes, yeast, streptomycete respectively, then be inoculated in yeast extract LB liquid nutrient medium respectively, 180-220 rev/min, leavening temperature 25-28 DEG C, fermentation time is 2-6 days, then by the single bacterium colony actinomycetes after fermentation, yeast, streptomycete, in single bacterium colony actinomycetes: yeast: the ratio of streptomycete=3:3:4 mixes, mixed solution is female flora;
2) preparation of compost flora seed fermentation agent:
1. female flora stoste step 1) prepared by volume 1:150-200 joins in sterile liquid yeast extract substratum, culture temperature 40-50 DEG C, 180rpm shaking culture 24h;
2. dry rice straw stalk is cut into about 3cm long, the dry rice straw cut short is mixed than 10:4 by mass/volume with water, vibration mixing, 121 DEG C, 20min sterilizing, must soak straw;
3. get above-mentioned steps nutrient solution 1. by volume 1:400-800 add in sterilized water, above-mentioned steps after 1:10-20 joins sterilizing by volume after shaking up 2. soak in straw, vibration stirs, and cultivates 7-10 days, i.e. amphimicrobian flora seed fermentation agent for 50 DEG C.
2. a preparation method for multifunctional microbial fertilizer according to claim 1, is characterized in that: the preparation method of this multifunctional microbial fertilizer:
One, the agent of amphimicrobian flora seed fermentation is prepared;
Two, agricultural organic waste raw material is prepared;
Three, the agriculture fertilizer of compost preparation fermentation:
(1) measure floor area, at least ensure that the waste materials heap of 45 cubes is high more than 1.5 meters, initial setting floor area is 15 square metres, spreads thin film in the above, on film, then spread the agent of skim amphimicrobian flora seed fermentation;
(2) at the agricultural organic waste raw material of the thick about 30-40cm in amphimicrobian flora seed fermentation agent upper berth, and add water and fully mix thoroughly, make water content be 55-65%, then spread the agent of one deck flora seed fermentation thereon, by that analogy, until stockpile is high more than 1.5 meters;
(3) in stockpile, cover one deck waterproof cloth and compacting, measure the temperature in 4 orientation, the four corners of the world with thermometer and note down;
(4) until raise waterproof cloth after compost 50-90 days, namely obtain by mixing the agriculture fertilizer that ferments;
Four, the multieffect biochemical fertilizer mixing that fermentation agricultural fertilizer step 3 obtained and Chinese patent ZL201210108437.8 provide, during mixing by mass percentage, ferment agriculture fertilizer 70-90%, multieffect biochemical fertilizer 10-30%, namely obtains multifunctional microbial fertilizer.
3. the preparation method of multifunctional microbial fertilizer according to claim 2, is characterized in that: described multifunctional microbial fertilizer 1-50 kilogram of plastic packaging bag packaging.
4. the preparation method of multifunctional microbial fertilizer according to claim 3, is characterized in that: described agricultural organic waste raw material is mixed to form by corn stalk 50% by mass percentage and cow dung 50%.
5. the preparation method of multifunctional microbial fertilizer according to claim 3, is characterized in that: described agricultural organic waste raw material is mixed to form by straw straw 50% by mass percentage and cow dung 50%.
6. the preparation method of multifunctional microbial fertilizer according to claim 3, is characterized in that: described agricultural organic waste raw material is mixed to form by straw straw 70 by mass percentage, weeds 10%, cow dung 20%.
CN201510168314.7A 2015-04-11 2015-04-11 Multifunctional microbial organic fertilizer and preparation method thereof Pending CN104761385A (en)

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CN105732143A (en) * 2016-03-09 2016-07-06 广东海洋大学 Method for producing organic fertilizer without pile turning
CN105753606A (en) * 2016-03-09 2016-07-13 广东海洋大学 Method of utilizing aquatic product viscera and oyster shells to prepare organic fertilizer
CN106034436A (en) * 2016-05-23 2016-10-26 葛洲坝中固科技股份有限公司 Method for recovering tilth of soil after thermal desorption
CN106187406A (en) * 2016-06-30 2016-12-07 哈尔滨尼亚农业有限公司 A kind of soil conditioner prevented erosion and preparation method
CN106478167A (en) * 2016-10-10 2017-03-08 南充禾香生物科技有限公司 Organic fermentation fertilizer and preparation method thereof
CN108794209A (en) * 2018-08-28 2018-11-13 阜阳市殿兴农业科技有限公司 A kind of muskmelon plantation organic fertilizer
CN110862278A (en) * 2019-12-06 2020-03-06 曲靖市土壤肥料工作站(曲靖市土壤肥料测试中心) Facultative anaerobe biological fertilizer

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CN108794209A (en) * 2018-08-28 2018-11-13 阜阳市殿兴农业科技有限公司 A kind of muskmelon plantation organic fertilizer
CN110862278A (en) * 2019-12-06 2020-03-06 曲靖市土壤肥料工作站(曲靖市土壤肥料测试中心) Facultative anaerobe biological fertilizer

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