CN108640751A - A kind of microbial manure, preparation method and application for preventing Radix Angelicae Sinensis root rot - Google Patents
A kind of microbial manure, preparation method and application for preventing Radix Angelicae Sinensis root rot Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The present invention relates to biological fertilizer fields, specifically, providing a kind of microbial manure for preventing Radix Angelicae Sinensis root rot comprising raw material：Pythium oligandrum and fertilizer；Wherein, pythium oligandrum and fertilizer mixed fermentation.Microbial manure provided by the present invention for preventing Radix Angelicae Sinensis root rot can be by pythium oligandrum and fertilizer mixed fermentation, it is used as carrier by fertilizer, pythium oligandrum is applied in root rot prevention in large quantities, so that plant is during continuous cropping, Soil Micro-environment changes less, is remarkably improved the control rate of plant root disease.In addition, the present invention also provides the preparation method of mentioned microorganism fertilizer and its application, to achieve the purpose that improve the control rate of plant root rot.
Description
Technical field
The present invention relates to biological fertilizer fields, in particular to a kind of microorganism fertilizer for preventing Radix Angelicae Sinensis root rot
Material, preparation method and application.
Background technology
Radix Angelicae Sinensis (Angelica sinensis (Oliv.) Diels), is also named that Min returns, the Qin is returned, and has enriching yin, enriches blood, carries
Many pharmacological effects such as high immunity.In recent years, the continuous excavation with people to Radix Angelicae Sinensis pharmacological active substance, economic value
It highlights, leads to rush of demand of the market to Radix Angelicae Sinensis resource.Currently, on the main producing region Gansu, Yunnan, Ningxia of China's Radix Angelicae Sinensis and other places,
People meet apparent availability by way of artificial growth, still, with the continuous expansion of cultivated area, inevitably
There are the continuous cropping plantation of Radix Angelicae Sinensis, and the thus Soil-sickness Problems such as caused Radix Angelicae Sinensis root rot, drastically influences Radix Angelicae Sinensis production
The sustainable development of industry.
As the major virulent factor reaping hook category bacterium (Fusarium Link ex Fr.) of Radix Angelicae Sinensis root rot, to its prevention and control one
It is directly difficult point and emphasis, main reason is that：Reaping hook category bacterium derives from a wealth of sources, and substantially any plant and soil etc. all carry
Various sickle-like bacteria, it is difficult to realize thoroughly radical cure；Reaping hook category bacterium can produce spore, stronger to the tolerance of environment, to chemical pesticide or
It is that physics burns the methods of topsoil not very sensitivity；Reaping hook category bacterium hobby is moist, Acid soil, and the attribute of soil with
Tillage method and local weather etc. are related, prevent easily by ectocine.Therefore, it is difficult to effectively solve reaping hook category with conventional method
The soil-borne disease problem that cingula is come.
Invention content
The first object of the present invention is to provide a kind of microbial manure for preventing Radix Angelicae Sinensis root rot, can will be few
Male rotten mould and fertilizer mixed fermentation, is used as carrier by fertilizer, pythium oligandrum is applied in the prevention of Radix Angelicae Sinensis root-rot so that Radix Angelicae Sinensis
During continuous cropping, Soil Micro-environment changes less, is remarkably improved the control rate of Radix Angelicae Sinensis root rot.
The second object of the present invention is to provide the preparation method of mentioned microorganism fertilizer, can make and be remarkably improved
The microbial manure of Radix Angelicae Sinensis root rot control rate.
The third object of the present invention is to provide application of the mentioned microorganism fertilizer in agricultural planting, can improve and work as
Return the control rate of root rot.
The fourth object of the present invention is to provide application of the mentioned microorganism fertilizer in treating Radix Angelicae Sinensis root rot, can
Improve the control rate of Radix Angelicae Sinensis root rot.
The invention is realized in this way：
A kind of microbial manure for preventing Radix Angelicae Sinensis root rot comprising raw material：Pythium oligandrum and fertilizer；Wherein, few
Male rotten mould and fertilizer mixed fermentation.
A kind of preparation method of such as above-mentioned microbial manure, by pythium oligandrum and fertilizer mixed fermentation.
Application of the above-mentioned microbial manure in agricultural planting.
Application of the mentioned microorganism fertilizer in treating Radix Angelicae Sinensis root rot.
The invention has the advantages that：
A kind of microbial manure for preventing Radix Angelicae Sinensis root rot of offer of the present invention comprising raw material：Pythium oligandrum and
Fertilizer；Wherein, pythium oligandrum and fertilizer mixed fermentation.Microbial manure provided by the invention can mix pythium oligandrum and fertilizer
Fermentation is closed, carrier is used as by fertilizer, is largely applied to pythium oligandrum in root-rot prevention so that process of the plant in continuous cropping
In, Soil Micro-environment changes less, is remarkably improved the control rate of plant root rot.In addition, the present invention also provides above-mentioned micro- lifes
The preparation method of object fertilizer and its application, to achieve the purpose that improve the control rate of plant root rot.
Specific implementation mode
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
The microbial manure of the embodiment of the present invention is specifically described below.
The embodiment of the present invention provides a kind of microbial manure for preventing Radix Angelicae Sinensis root rot comprising raw material：It is few male rotten
Mould and fertilizer；Wherein, pythium oligandrum and fertilizer mixed fermentation.
Specifically, pythium oligandrum is that a kind of very strong parasitical fungi of adaptability, pythium oligandrum present in nature pass through
Pathomycete is effectively killed in parasitization, inhibits the spore germination of pathomycete, mycelia to grow by antibiosis, to reach
The purpose of preventing and treating diseases；It can be colonized on root system of plant surface, and can enter root system vascular bundle, so as to be effectively prevented and treated
Root system of plant disease；It enters the process of root system and promotes root system of plant cell wall thickening, secretion oligandrin that can stimulate plant
The generation of object disease resistance mechanisms itself improves plant disease-resistant ability, occurs to achieve the purpose that reduce various diseases；Pythium oligandrum
The class auxin compound of secretion promotes the synthesis of auximone, a variety of ectoenzymes of secretion to decompose the phosphorus in soil, to
Achieve the effect that promote root growth, increase crop yield.
Further, pythium oligandrum is 3~7 parts, and fertilizer is 93~97 parts.The microorganism fertilizer being mixed to prepare using the ratio
Material has the pythium oligandrum being easy in fertilizer amplification, improves control rate of the microbial manure to inhibition Radix Angelicae Sinensis root rot.
Further, above-mentioned pythium oligandrum is pythium oligandrum conidia powder or pythium oligandrum bacterium solution.
Further, fertilizer can be organic fertilizer, and organic fertilizer includes cake fertilizer, compost, make compost, barnyard manure, natural pond fertilizer and green manure
In it is one or more.Specifically, organic fertilizer refers to being rich in organic substance, nutrient needed for crop growth can be provided, is improved
Soil, and a kind of fertilizer that crop yield can be improved, keep soil laborious.Wherein, cake fertilizer refers to having soya-bean cake, rapeseed cake, pockmarks
Cake, cotton cake, peanut cake, seeds of a tung oil tree cake, camellia oleosa seed cake etc.；Compost refers to all kinds of tangerine stalks, fallen leaves, green grass, plant and animal residues, people and animals
Excrement is raw material, with a kind of organic fertilizer made of a small amount of soil mixed-stacking；Make compost is natural organic matter through microorganism decomposition
Or the ferment a kind of fertilizer formed, also known as farm manure, raw material sources are wide, and quantity is big, and nutrient is complete, and content is low, and fertilizer efficiency is grown late,
It must could be absorbed by plant after microorganism decomposition converts；Barnyard manure refer to domestic animal (pig, ox, horse, sheep, chicken, duck etc.) fecaluria and
Gasket materials, feed stubble mixed-stacking and through fertilizer made of microbial action；Natural pond fertilizer is referring to biogas slurry and biogas residue composition, biogas slurry
And control slag and be collectively referred to as natural pond fertilizer, it is product of the biomass through methane-generating pit anaerobic fermentation；Green manure refers to using green plants body (such as pulse family
Mung bean, broad bean, daghestan sweet clover, sesbania, clover, sweet potato etc..Nonleguminous green manure has rye grass, Radish, small certain herbaceous plants with big flowers, Azolla imbricata, water
Cucurbit, water peanut etc.) made of fertilizer, green manure is a kind of nutrient completely biological source of manure.
The present invention provides a kind of preparation method of mentioned microorganism fertilizer comprising step：Pythium oligandrum and fertilizer are mixed
Close fermentation.
Further, before pythium oligandrum is mixed with fertilizer, pythium oligandrum can individually be fermented, obtains pythium oligandrum fermentation
Liquid, then by pythium oligandrum zymotic fluid and fertilizer mixed fermentation.In other words, few male rotten in the preparation method of microbial manure
Mould to pass through secondary fermentation, secondary fermentation can increase the secretion of pythium oligandrum, improve the disease-resistant rate of microbial manure, more preferably
Ground promotes plant growth.
Further, individually fermentation refers to that pythium oligandrum is placed on fermentation medium and ferments to above-mentioned pythium oligandrum,
Fermentation medium includes following component：Potato 19%~21%, peptone 0.4%~0.6%, sodium chloride 0.4%~
0.6%.
Further, in the fermentation medium, pythium oligandrum can be added to fermented and cultured with 2%~5% ratio
In base.
The present invention also provides application of the mentioned microorganism fertilizer in agricultural planting.Specially in Radix Angelicae Sinensis, it is blended with
The microbial manure of pythium oligandrum is contacted with soil, can be provided nutrient to Radix Angelicae Sinensis and be inhibited the generation of Radix Angelicae Sinensis root rot.
The present invention also provides application of the mentioned microorganism fertilizer in treating Radix Angelicae Sinensis root rot.Specifically, the present invention is carried
The microbial manure of confession is contacted with there is the root system of the Radix Angelicae Sinensis of root rot.
The feature and performance of the present invention are described in further detail with reference to embodiments.
First embodiment
The present embodiment provides a kind of preparation methods for preventing the microbial manure of Radix Angelicae Sinensis root rot.
The preparation of pythium oligandrum conidia powder
Pythium oligandrum is put into test tube and carries out inclined-plane culture (+15% agar of potato dextrose agar), is waited for
After covering with spore on slant medium, slant tube is injected per effective 10mL sterile salines on super-clean bench, is connect with sterile
Kind ring touches chamfered surface, so that spore is fully eluted mixing, obtains eluent.The eluent is filtered to take with aseptic filter paper
Spore suspension, then the spore suspension is abandoned into supernatant concentration to about 2mL through 6000r/min centrifugations, it is seeded to equipped with 20mL
First order seed shake flask culture (glucose 2%, peptone 1%, sodium chloride 0.5%, 0.1Mpa, 121 DEG C sterilizing 20min after it is cold
But it in 100mL triangular flasks), in cultivating 48h on 25 DEG C~28 DEG C, the shaking table of rotating speed 180r/min, is then transferred to and is equipped with
100mL secondary seeds culture solution (sucrose 2%, peptone 1%, sodium chloride 0.5%, 0.1Mpa, 121 DEG C sterilizing 20min after it is cold
But in 250mL shaking flasks), 48h is cultivated on 25 DEG C, the shaking table of rotating speed 180r/min.Spore suspension is transferred to 50L gas lifts later
In formula fermentation tank culture medium (potato 20%, peptone 0.5%, sodium chloride 0.5%), 25 DEG C of temperature, ventilatory capacity 1.0vvm is stirred
Mix rotating speed 120r/min fermentations 48h obtains bacterium solution.The bacterium solution of aerial fermentation tank culture is transferred to rotary drum solid fermentation again
(culture medium of built-in sterilized cubical content 30%, group become in tank：Soya-bean cake 5%, corn flour 10%, wheat bran 50%, straw powder
35%, the wherein mass ratio of solid material and moisture is 3:2) preceding 3 days temperature of, fermenting are 28 DEG C, and 4d plays 3 DEG C of trainings of cooling daily
It supports, until terminating when fermentation 6d, gained is pythium oligandrum archesporium powder preparation, living bacteria count 6 × 108A/g.
The preparation of organic fertilizer
Carbon-nitrogen ratio (25 will be adjusted:1) and the material of water content 55% (maize straw+cattle and sheep excrement mixture), according to
Organic material composting microbial inoculum is added in 1 ‰ ratio, stirs and evenly mixs；The material of mixing is piled into trapezoidal fermentation, 1 meter of heap top width, heap
2 meters of bottom width, heap is 0.8~1 meter high, and the length of windrow is determined according to inventory, and summer pays attention to avoiding sunlight direct irradiation；Fermentation is opened
After beginning, temperature of charge is detected daily, the turning when rising to 60 DEG C or more away from 30 centimeters temperature of charge of surface layer, and repeat this mistake
Journey, until when heap temperature is on a declining curve within the time for three days on end, this process is usually 10 days.
The preparation of microbial manure
The conidia powder of above-mentioned preparation is added to 2%~5% ratio in fermentation medium, then with 3% ratio (spore
3 parts of sub- powder, 93 parts of organic fertilizer) it is seeded in above-mentioned organic fertilizer, or the biology for having bacteriostasis is made after stacking or airing 3d
Organic fertilizer.
Second embodiment
The present embodiment provides a kind of preparation methods for preventing the microbial manure of Radix Angelicae Sinensis root rot, with first embodiment
The preparation method of offer is substantially the same, as follows difference lies in the variation of parameter：
The preparation of pythium oligandrum conidia powder
Pythium oligandrum is put into test tube and carries out inclined-plane culture (+15% agar of potato dextrose agar), is waited for
After covering with spore on slant medium, slant tube is injected per effective 10mL sterile salines on super-clean bench, is connect with sterile
Kind ring touches chamfered surface, so that spore is fully eluted mixing, obtains eluent.The eluent is filtered to take with aseptic filter paper
The spore suspension is abandoned supernatant concentration to about 2mL through 7000r/min centrifugations, is seeded to equipped with 20mL mono- by spore suspension
Grade seed flask culture solution (glucose 2%, peptone 1%, sodium chloride 0.5%, 0.1Mpa, 121 DEG C of sterilizing 20min postcoolings)
100mL triangular flasks in, in cultivating 48h on 26 DEG C, the shaking table of rotating speed 180r/min, be then transferred to equipped with 100mL two level kinds
The 250mL of sub- culture solution (sucrose 2%, peptone 1%, sodium chloride 0.5%, 0.1Mpa, 121 DEG C of sterilizing 20min postcoolings) shakes
In bottle, 48h is cultivated on 26 DEG C, the shaking table of rotating speed 180r/min.Spore suspension is transferred to 50L airlift fermentor cultures later
In base (potato 20%, peptone 0.5%, sodium chloride 0.5%), 26 DEG C, ventilatory capacity 1.0vvm, speed of agitator 120r/ of temperature
Min fermentations 48h obtains bacterium solution.The bacterium solution of aerial fermentation tank culture is transferred to again (built-in to go out in rotary drum solid-state fermentation tank
The culture medium of the cubical content 30% of bacterium is crossed, group becomes：Soya-bean cake 5%, corn flour 10%, wheat bran 50%, straw powder 35%, wherein solid
The mass ratio of body material and moisture is 3:2) preceding the 3 days temperature of, fermenting are 28 DEG C, and 4d plays 3 DEG C of cultures of cooling daily, until fermenting the
Terminate when 6d, gained is pythium oligandrum archesporium powder preparation, living bacteria count 6 × 108A/g.
The preparation of organic fertilizer
Carbon-nitrogen ratio (27 will be adjusted:1) it with the material of water content (58%) (maize straw+cattle and sheep excrement mixture), presses
Organic material composting microbial inoculum is added according to 3 ‰ ratio, stirs and evenly mixs；The material of mixing is piled into trapezoidal fermentation, 1 meter of heap top width,
2 meters of heap bottom width, heap is 0.9 meter high, and the length of windrow is determined according to inventory, and summer pays attention to avoiding sunlight direct irradiation；Fermentation is opened
After beginning, temperature of charge is detected daily, the turning when rising to 60 DEG C or more away from 40 centimeters temperature of charge of surface layer, and repeat this mistake
Journey, until when heap temperature is on a declining curve within the time for three days on end, this process is 10 days.
The preparation of microbial manure
The conidia powder of above-mentioned preparation is added to 3% ratio in fermentation medium, then is seeded to 4% ratio
It states in organic fertilizer (4 parts of conidia powder, 95 parts of organic fertilizer), the biological organic fertilizer for having bacteriostasis is made after stacking 4d.
3rd embodiment
It is real with first and second the present embodiment provides a kind of preparation method for preventing the microbial manure of Radix Angelicae Sinensis root rot
The preparation method for applying example offer is substantially the same, as follows difference lies in the variation of parameter：
The preparation of pythium oligandrum conidia powder
Pythium oligandrum is put into test tube and carries out inclined-plane culture (+15% agar of potato dextrose agar), is waited for
After covering with spore on slant medium, slant tube is injected per effective 10mL sterile salines on super-clean bench, is connect with sterile
Kind ring touches chamfered surface, so that spore is fully eluted mixing, obtains eluent.The eluent is filtered to take with aseptic filter paper
Spore suspension, then the spore suspension is abandoned into supernatant concentration to about 2mL through 8000r/min centrifugations, it is seeded to equipped with 20mL
First order seed shake flask culture (glucose 2%, peptone 1%, sodium chloride 0.5%, 0.1Mpa, 121 DEG C sterilizing 20min after it is cold
But it in 100mL triangular flasks), in cultivating 48h on 28 DEG C, the shaking table of rotating speed 180r/min, is then transferred to equipped with 100mL two levels
The 250mL of seed culture fluid (sucrose 2%, peptone 1%, sodium chloride 0.5%, 0.1Mpa, 121 DEG C of sterilizing 20min postcoolings)
In shaking flask, 48h is cultivated on 28 DEG C, the shaking table of rotating speed 180r/min.Spore suspension is transferred to the training of 50L airlift fermentors later
It supports in base (potato 20%, peptone 0.5%, sodium chloride 0.5%), 28 DEG C, ventilatory capacity 1.0vvm of temperature, speed of agitator
120r/min fermentations 48h obtains bacterium solution.The bacterium solution of aerial fermentation tank culture is transferred to again (interior in rotary drum solid-state fermentation tank
The culture medium of sterilized cubical content 30% is filled, group becomes：Soya-bean cake 5%, corn flour 10%, wheat bran 50%, straw powder 35%,
The mass ratio of middle solid material and moisture is 3:2) preceding 3 days temperature of, fermenting are 28 DEG C, and 4d plays 3 DEG C of cultures of cooling daily, until hair
Terminate when ferment 6d, gained is pythium oligandrum archesporium powder preparation, living bacteria count 6 × 108A/g.
The preparation of organic fertilizer
Carbon-nitrogen ratio (30 will be adjusted:1) it with the material of water content (60%) (maize straw+cattle and sheep excrement mixture), presses
Organic material composting microbial inoculum is added according to 5 ‰ ratio, stirs and evenly mixs；The material of mixing is piled into trapezoidal fermentation, 1 meter of heap top width,
2 meters of heap bottom width, heap is 1 meter high, and the length of windrow is determined according to inventory, and summer pays attention to avoiding sunlight direct irradiation；Fermentation starts
Afterwards, temperature of charge is detected daily, the turning when rising to 60 DEG C or more away from 50 centimeters temperature of charge of surface layer, and repeat this mistake
Journey, until when heap temperature is on a declining curve within the time for three days on end, this process is usually 15 days.
The preparation of microbial manure
The conidia powder of above-mentioned preparation is added to 5% ratio in fermentation medium, then is seeded to 5% ratio
It states in organic fertilizer (5 parts of conidia powder, 97 parts of organic fertilizer), the biological organic fertilizer for having bacteriostasis is made after airing 5d.
Fourth embodiment
Microbial manure made from the preparation method that the first~tri- embodiment provides is verified in the plantation of continuous cropping Radix Angelicae Sinensis to soil
The influence of earth and Radix Angelicae Sinensis growing state.
1. experimental method
One piece of Minxian County pockmarks river township is chosen at 2014 accounts for 5 mu of soil plantation Radix Angelicae Sinensis, fertilising common fertilizer (urea+phosphorus
Acid diamine), yield, Radix Angelicae Sinensis root rot incidence and soil pH value are measured when Radix Angelicae Sinensis harvests；The ground was divided into five pieces in 2015, if
Five processing, control experiment condition is as shown in table 1, and each processing is 1 mu or so, before Radix Angelicae Sinensis transplanting, in each processing plot
Fertilizer 90kg is uniformly applied, after Radix Angelicae Sinensis transplanting, applies fertilizer 15g again.
The control experiment condition of 1 fourth embodiment of table
Measure pythium oligandrum bacterium number in different disposal Rhizosphere Soil, Radix Angelicae Sinensis yield, soil pH respectively when current year Radix Angelicae Sinensis harvests
Value and root-rot disease incidence.
2. experimental result is as shown in table 2
Microbial manure in 2 first embodiment of table gives birth to soil and Radix Angelicae Sinensis in the plantation of Minxian County pockmarks river township's continuous cropping Radix Angelicae Sinensis
The influence of long situation
As shown in Table 2, few in microbial manure prepared by the preparation method provided using the first~tri- embodiment of the invention
Male pythium spp number is apparently higher than other two control groups, and Radix Angelicae Sinensis root rot evil is efficiently controlled, Radix Angelicae Sinensis yield
It increases, illustrates that microbial manure provided by the invention can effectively prevent the root rot of Radix Angelicae Sinensis and the life to Radix Angelicae Sinensis
With facilitation.
5th embodiment
Microbial manure made from the preparation method that the first~tri- embodiment provides is verified in the plantation of continuous cropping Radix Angelicae Sinensis to soil
The influence of earth and Radix Angelicae Sinensis growing state.
It is respectively ACCC that few male auxiliary mould, which uses the number introduced from Chinese agriculture Microbiological Culture Collection administrative center,
38048 pythium oligandrum bacterial strain.
1. experimental method
Selection of land is tested in the Gansu Minxian County towns Min Chuan, which takes up an area 4 mu, and common fertilizer is applied in Radix Angelicae Sinensis soil plantation in 2014
(urea+phosphoric acid diamines) measures yield, root rot incidence and soil pH value when Radix Angelicae Sinensis harvests；The ground was divided into eight in 2015
Block, if eight processing, control experiment condition is as shown in table 3, and each processing is 0.5 mu or so, before Radix Angelicae Sinensis transplanting, at each place
It manages plot and uniformly applies fertilizer 80kg, after Radix Angelicae Sinensis transplanting, apply fertilizer 15g again.
The control experiment condition of the 5th embodiment of table 3
Measure pythium oligandrum bacterium number in different disposal Rhizosphere Soil, Radix Angelicae Sinensis yield, soil pH respectively when current year Radix Angelicae Sinensis harvests
Value and root-rot disease incidence, test result such as table 4 show.
2. experimental result
Table 4 the 5th apply the microbial manure of example in the plantation of continuous cropping Radix Angelicae Sinensis to the influence knot of soil and Radix Angelicae Sinensis growing state
Fruit
According to table 4, the first~tri- embodiment of comparison is with the first reference examples it is found that few male auxiliary mould is directly mixed with fertilizer
Fermentation can be effectively increased the quantity of few male auxiliary mould, improve the control rate of Radix Angelicae Sinensis root rot and the yield of Radix Angelicae Sinensis.
The the first~tri- embodiment is compared with the second~tri- reference examples it is found that by the spore of the excessive or very few male auxiliary mould of widow
Powder is individually fermented, and all will generate bad shadow to the control rate of Radix Angelicae Sinensis root rot and the yield of Radix Angelicae Sinensis to microbial manure
It rings.
Compare the first~tri- embodiment and the four~five reference examples it is found that few male auxiliary mould and organic fertilizer proportioning, to right
The control rate of Radix Angelicae Sinensis root rot and the yield of Radix Angelicae Sinensis play an important role, and few male auxiliary mould is inoculated with 2%~7% ratio
Into organic fertilizer, the control rate of Radix Angelicae Sinensis root rot can be preferably improved, then improves the yield of Radix Angelicae Sinensis.
In conclusion a kind of microbial manure for preventing Radix Angelicae Sinensis root rot of offer of the present invention comprising raw material：It is few
The mould and fertilizer of male corruption；Wherein, pythium oligandrum and fertilizer mixed fermentation.Microbial manure provided by the invention can be by pythium oligandrum
With fertilizer mixed fermentation, carrier is used as by fertilizer, is largely applied to pythium oligandrum in root-rot prevention so that plant is even
During work, Soil Micro-environment changes less, is remarkably improved the control rate of plant root rot.In addition, the present invention also provides
The preparation method of mentioned microorganism fertilizer and its in agricultural planting and its application in treating Radix Angelicae Sinensis root rot, to reach
Improve the purpose of the control rate of plant root rot.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, any made by repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of microbial manure for preventing Radix Angelicae Sinensis root rot, which is characterized in that it includes raw material：Pythium oligandrum and fertilizer
Material；Wherein, the pythium oligandrum and the fertilizer mixed fermentation.
2. microbial manure according to claim 1, which is characterized in that the pythium oligandrum is 3~7 parts, the fertilizer
It is 93~97 parts.
3. microbial manure according to claim 1, which is characterized in that the pythium oligandrum be pythium oligandrum conidia powder or
Pythium oligandrum bacterium solution.
4. microbial manure according to claim 1, which is characterized in that the fertilizer is organic fertilizer, and the organic fertilizer includes
Cake fertilizer compost, makes compost, is in barnyard manure, natural pond fertilizer and green manure one or more.
5. a kind of preparation method of microbial manure as described in claim 1, which is characterized in that it includes step：It will be described
Pythium oligandrum and the fertilizer mixed fermentation.
6. preparation method according to claim 5, which is characterized in that, will before the pythium oligandrum is mixed with the fertilizer
The pythium oligandrum individually ferments, and obtains pythium oligandrum zymotic fluid, then mixes the pythium oligandrum zymotic fluid and the fertilizer
Close fermentation.
7. preparation method according to claim 6, which is characterized in that state pythium oligandrum individually fermentation refer to will be described few male
Rotten mould be placed on fermentation medium is fermented, and the fermentation medium includes following component：Potato 19%~21%, egg
White peptone 0.4%~0.6%, sodium chloride 0.4%~0.6%.
8. preparation method according to claim 7, which is characterized in that add the pythium oligandrum with 2%~7% ratio
It adds in the fermentation medium.
9. application of the Claims 1 to 4 any one of them microbial manure in agricultural planting.
10. application of Claims 1 to 4 any one of them microbial manure in treating Radix Angelicae Sinensis root rot.
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