CN108034596B - One Rhodotorula mucilaginose strain strain, peanut drop cadmium agent and its application - Google Patents
One Rhodotorula mucilaginose strain strain, peanut drop cadmium agent and its application Download PDFInfo
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- CN108034596B CN108034596B CN201810086693.9A CN201810086693A CN108034596B CN 108034596 B CN108034596 B CN 108034596B CN 201810086693 A CN201810086693 A CN 201810086693A CN 108034596 B CN108034596 B CN 108034596B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Abstract
The present invention provides a Rhodotorula mucilaginose strain strain, peanut drop cadmium agent and its applications, belong to Crop securify production technical field.Peanut drop cadmium agent provided by the invention including rhodotorula mucilaginosa fermentation liquid or rhodotorula mucilaginosa solid pharmaceutical preparation can reduce the content of cadmium in Ecological Property of Peanut Seeds.P content, Ecological Property of Peanut Seeds yield and the Peanut Root System biomass in blade can also be improved simultaneously.Drop cadmium agent provided by the invention further includes the sulfosalisylic acid solution that mass concentration is 1~2g/L, can further increase the removal rate to cadmium in Ecological Property of Peanut Seeds.Compared with not applying drop cadmium agent, peanut is cultivated using drop cadmium agent provided by the invention, cadmium content in Ecological Property of Peanut Seeds is set to reduce by 40.63%~44.78%, phosphorus content increases by 18.18%~21.43% simultaneously, Peanut Root System dry weight improves 31.13%~37.9%, Ecological Property of Peanut Seeds output increased 20.85%~22.2%.
Description
Technical field
The invention belongs to Crop securify production technical fields, and in particular to cadmium agent drops in a Rhodotorula mucilaginose strain strain, peanut
And its application.
Background technique
With the development of industry with the modernization of agricultural production, heavy metals in farmland, which pollutes, to be got worse, and contaminated area is year by year
Expand.Heavy metal is due to having many characteristics, such as that not degradable, easy enrichment receives significant attention.Because having high toxicity and high migration,
Cadmium (Cd) is considered as most important environmental pollutants, and excessive cadmium can lead to Itai-itai diseases, kidney trouble, liver damage in human body
A series of diseases such as evil.After cadmium enters soil, preferentially in conjunction with carbonate, the cadmium carbonate of indissoluble is formed, there is biggish movement
Property and plant availability to be easier to be absorbed by plants into food chain harm is generated to human health.
Peanut is to inhale one of strongest field crop of cadmium ability, the absorption to cadmium in soil and the efficiency to seed transfer
Higher than many crops.According to investigations, Ecological Property of Peanut Seeds cadmium average content in northern China producing region is 0.2-0.3mg/kg, investigates the soil in place
For earth without obvious pollution condition, majority has exceeded the nuisanceless peanut Cd content 0.05mg/kg (NY5303-2005) of country, portion
Dividing has been more than green food standard 0.4mg/kg limitation (NY/T420-2009).Ecological Property of Peanut Seeds cadmium content is exceeded to have become restriction
An important factor for outlet of China's peanut and related industry development.
Currently, selecting special crop varieties, screening or breeding low cadmium-accumulation kind are abatement plant edible part cadmium accumulations
A kind of mode, but such mode, due to the particularity of plant variety, there may be certain defects, such as yield for biological character
It is low, resistance is poor etc., limit being widely applied for kind.
Summary of the invention
In view of this, the purpose of the present invention is to provide a Rhodotorula mucilaginose strain strains, the system of reduction Ecological Property of Peanut Seeds cadmium content
Agent can reduce Ecological Property of Peanut Seeds Cd accumulation, improves Ecological Property of Peanut Seeds and drops cadmium efficiency.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of rhodotorula mucilaginosa bacterial strain Rhodotorula mucilaginosa OP11, deposit number is
CGMCCNo.13540。
It cultivates peanut the present invention also provides one and drops cadmium agent, including rhodotorula mucilaginosa fermentation liquid or rhodotorula mucilaginosa solid pharmaceutical preparation；
The preparation method of the rhodotorula mucilaginosa fermentation liquid, comprising the following steps:
Rhodotorula mucilaginosa bacterial strain described in above scheme is activated and expands culture, obtains rhodotorula mucilaginosa bacterial strain fermentation seed liquid,
The rhodotorula mucilaginosa bacterial strain fermentation seed liquid is inoculated into malt juice liquid medium by 2.5%~5% inoculum concentration, 25
~30 DEG C, liquid fermentation 72h~96h under conditions of 150~200r/min of revolving speed obtains rhodotorula mucilaginosa fermentation liquid；
The preparation method of the rhodotorula mucilaginosa solid pharmaceutical preparation, comprising the following steps:
A. rhodotorula mucilaginosa bacterial strain described in above scheme is activated and expands culture, obtains rhodotorula mucilaginosa bacterial strain fermentation seed
The rhodotorula mucilaginosa bacterial strain fermentation seed liquid is inoculated into wheat bran medium by 5%~10% inoculum concentration 28~30 by liquid
5~7d of solid fermentation under the conditions of DEG C, stirring, obtains mixture；
B. the obtained mixture of the step a is continued at 28~30 DEG C 3~5d of fermentation, obtains rhodotorula mucilaginosa solid system
Agent.
Preferably, it is the sulfosalisylic acid solution of 1~2g/L that the drop cadmium agent, which further includes the mass concentration independently dispensed,.
The present invention provides a kind of application of the drop cadmium agent of peanut described in above method in plantation peanut.
Preferably, the proline solution seed soaking of 15~45mmol/L is used before peanut cultivation.
Preferably, the rhodotorula mucilaginosa fermentation liquid is applied in the rhizosphere of plantation peanut；The rhodotorula mucilaginosa fermentation liquid is applied
Dosage is 16~20L/ mus.Preferably, the application number of the rhodotorula mucilaginosa fermentation liquid is 3 times；The time of application is for the first time
14~16d after peanut emergence, second time for applying are 29~31d after peanut emergence, and the time that third time applies is
44~46d after peanut emergence.
Preferably, the rhodotorula mucilaginosa solid pharmaceutical preparation is applied in the soil surface of plantation peanut；The rhodotorula mucilaginosa solid
The applied amount of preparation is 8~10kg/ mus.
Preferably, the application number of the rhodotorula mucilaginosa solid pharmaceutical preparation is 1 time, time of application is 7 before peanut seeding~
10d。
Preferably, sulfosalisylic acid solution is sprayed on the blade face of plantation peanut in peanut florescence, every 9~11d is sprayed
Once, continuous spraying is three times.
Preferably, each amount of spraying of the sulfosalisylic acid solution is 8~12L/ mus.
The present invention provides a Rhodotorula mucilaginose strain strain Rhodotorula mucilaginosa OP11, deposit number is
CGMCC No.13540.Cadmium agent energy drops in the peanut provided by the invention including rhodotorula mucilaginosa fermentation liquid or rhodotorula mucilaginosa solid pharmaceutical preparation
The content of cadmium in Ecological Property of Peanut Seeds is enough reduced, rhodotorula mucilaginosa fermentation liquid and solid pharmaceutical preparation improve peanut phosphorus nourishing situation, promote
Peanut growth reduces absorption of the peanut to Cd, while reducing Cd and being shifted from root system to seed, to reduce Ecological Property of Peanut Seeds
In Cd content.Not apply drop cadmium agent as a control group, applying drop cadmium agent can be significantly reduced Ecological Property of Peanut Seeds Cd content.Apply
Cd content is lower than the Cd content 28.14%~29.869% of control group in the Ecological Property of Peanut Seeds planted after drop cadmium agent.
Meanwhile drop cadmium agent provided by the invention can also dramatically increase the content of blade P.In the peanut leaf for applying drop cadmium agent
P content is higher than 14.55%~16.1% of P content in control group.In addition, drop cadmium agent provided by the invention can also improve peanut
Grain yield and Peanut Root System biomass.Compared with the control group, apply drop cadmium agent weight of root system it is higher than control group by 20.58%~
30.3%, grain yield is higher than control group by 17.2%~17.7%.
Further, drop cadmium agent provided by the invention contains the sulfosalisylic acid solution for including mass concentration for 1~2g/L.It will
Rhodotorula mucilaginosa bacterial strain preparation and mass concentration described in above scheme are that the sulfosalisylic acid solution of 1~2g/L is used cooperatively.Glue
Rhodotorula bacterial strain preparation effectively improves peanut phosphorus nourishing situation, and sulfosalisylic acid solution improves peanut to the resistance of Cd, promotees
Into peanut growth, the two can further decrease absorption of the peanut to Cd after being used cooperatively, while reduce Cd from root system to seed
It is shifted, so as to further reduce the content of cadmium in Ecological Property of Peanut Seeds, plants Ecological Property of Peanut Seeds after applying the preparation
Middle Cd content is lower than control group 40.63%~44.78%.
Meanwhile the peanut provided by the invention including the sulfosalisylic acid solution that mass concentration is 1~2g/L drops cadmium agent also
The content of blade P can be further increased, compared with the control group, blade P content is higher than control group 18.18%~21.43%.It applies
After adding peanut to drop cadmium agent, weight of root system is higher than control group by 31.13%~37.9%, and grain yield is higher than control group by 20.85%~
22.2%.
In addition, the present invention is soaked seed before peanut cultivation using proline, it can be improved seed resistance, make the emergence rate of peanut
Improve 15~25%.
Biological deposits explanation
Rhodotorula mucilaginosa (Rhodotorula mucilaginosa) is preserved in China Microbiological bacterial strain preservation administration committee
Common micro-organisms center, preservation address Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation mechanism abbreviation: CGMCC, preservation day
Phase is on 01 06th, 2017, and biological deposits number is CGMCC No.13540, strain number: OP11.
Specific embodiment
The present invention provides a kind of rhodotorula mucilaginosa bacterial strain Rhodotorula mucilaginosa OP11, deposit number is
CGMCCNo.13540。
It cultivates peanut the present invention also provides one and drops cadmium agent, including rhodotorula mucilaginosa fermentation liquid or rhodotorula mucilaginosa solid pharmaceutical preparation；
The preparation method of the rhodotorula mucilaginosa fermentation liquid, comprising the following steps:
Rhodotorula mucilaginosa bacterial strain described in above scheme is activated and expands culture, obtains rhodotorula mucilaginosa bacterial strain fermentation seed liquid,
The rhodotorula mucilaginosa bacterial strain fermentation seed liquid is inoculated into malt juice liquid medium by 2.5%~5% inoculum concentration, 25
~30 DEG C, liquid fermentation 72h~96h under conditions of 150~200r/min of revolving speed obtains rhodotorula mucilaginosa fermentation liquid；
The preparation method of the rhodotorula mucilaginosa solid pharmaceutical preparation, comprising the following steps:
A. rhodotorula mucilaginosa bacterial strain described in above scheme is activated and expands culture, obtains rhodotorula mucilaginosa bacterial strain fermentation seed
The rhodotorula mucilaginosa bacterial strain fermentation seed liquid is inoculated into wheat bran medium by 5%~10% inoculum concentration 28~30 by liquid
5~7d of solid fermentation under the conditions of DEG C, stirring, obtains mixture；
B. the obtained mixture of the step a is continued at 28~30 DEG C 3~5d of fermentation, obtains rhodotorula mucilaginosa solid system
Agent.
The present invention preferably activates rhodotorula mucilaginosa bacterial strain, the rhodotorula mucilaginosa bacterial strain after being activated.The activation is used
Culture medium is brewer's wort slant medium.The preparation method of the brewer's wort slant medium are as follows: weigh brewer's wort 20g, albumen
Peptone 1g, glucose 20g, agar 18g are settled to 1000ml, 121 DEG C of sterilizing 20min with deionized water, obtain the training of brewer's wort inclined-plane
Support base.The temperature of the activation is preferably 28~32 DEG C, and more preferably 30 DEG C.The time of the activation is preferably 36~52h, more
Preferably 48h.
In the present invention, after the rhodotorula mucilaginosa bacterial strain after being activated, the present invention is preferably by the rhodotorula mucilaginosa after the activation
Bacterial strain expands culture, and obtains rhodotorula mucilaginosa fermentation seed liquid.The mode for expanding culture are as follows: after the activation of one ring of picking
Rhodotorula mucilaginosa be linked into malt juice liquid medium under conditions of 29~31 DEG C, 170~190r/min of revolving speed culture 44~
52h.The temperature for expanding culture is preferably 30 DEG C.The revolving speed is preferably 180r/min.The time of the culture is preferably
48h。
After obtaining rhodotorula mucilaginosa fermentation seed liquid, the present invention connects the rhodotorula mucilaginosa fermentation seed liquid by 2.5%~5%
Kind of amount be inoculated into malt juice liquid medium under conditions of 25~30 DEG C, 150~200r/min of revolving speed liquid fermentation 72h~
96h obtains rhodotorula mucilaginosa fermentation liquid.Viable count is 9 × 10 in obtained rhodotorula mucilaginosa fermentation liquid6~6 × 108CFU/mL。
In the present invention, the inoculum concentration of the rhodotorula mucilaginosa fermentation seed liquid is preferably 4%.The temperature of the liquid fermentation is excellent
It is selected as 28 DEG C.The time of the liquid fermentation is preferably 85h.The revolving speed when liquid fermentation is preferably 180r/min.
In the present invention, the preparation method of the malt juice liquid medium are as follows: weigh brewer's wort 20g, peptone 1g, grape
Sugared 20g is settled to 1000mL with deionized water, and 121 DEG C of sterilizing 20min obtain brewer's wort slant medium, obtain mashing water
Body culture medium.
The present invention is not particularly limited the source of each component in the malt juice liquid medium, using this field routine
Commercial product.Brewer's wort slant medium and fluid nutrient medium each component are purchased from Chinese medicines group in the embodiment of the present invention
Learn reagent Beijing Co., Ltd.
In the present invention, the rhodotorula mucilaginosa fermentation liquid improves peanut phosphorus nourishing situation, promotes peanut growth, reduces flower
The raw absorption to Cd, while reducing Cd and being shifted from root system to seed, to reduce the Cd content in Ecological Property of Peanut Seeds.
When preparing rhodotorula mucilaginosa solid pharmaceutical preparation, inoculum concentration of the rhodotorula mucilaginosa fermentation seed liquid by 5%~10% will be obtained
It is inoculated into the wheat bran medium solid fermentation 5~7d of culture under the conditions of 25~30 DEG C, stirs, obtains mixture.This hair
In bright, the inoculum concentration is preferably 8%.The solid fermentation cultivation temperature is preferably 28 DEG C.The solid fermentation incubation time
Preferably 6d.
After obtaining mixture, the mixture is continued 3~5d of solid fermentation by the present invention at 28~30 DEG C, and it is red to obtain glue
Yeast solids preparation.
After obtaining rhodotorula mucilaginosa solid pharmaceutical preparation, the present invention preferably stirs the rhodotorula mucilaginosa solid pharmaceutical preparation, 25~30
The operation of 1.5~2.5d of solid fermentation repeats 2~3 times at DEG C, is uniformly distributed thallus in wheat bran medium.
In the present invention, the mixed mode is preferably stirred.The revolving speed of the stirring is preferably 150~200r/min,
More preferably 180r/min.The time of the stirring is preferably 3~5min, more preferably 4min.The time of the fermentation is preferred
For 2d.
In the present invention, percentage, the component of the wheat bran medium are as follows: 87.9 parts of wheat bran, yeast powder 1.6
Part, 8.0 parts of rice bran, KH2PO40.5 part, FeSO40.3 part, (NH4)2SO41.1 parts and MgSO40.6 part.
The water content of the wheat bran medium is preferably 40%~60wt%, more preferably 50wt%.The wheat bran culture
Base preferably sterilizes before inoculation.The sterilising conditions temperature is 121 DEG C.The sterilization time is preferably 30min.
The present invention is not particularly limited the source of the wheat bran medium, using this field conventional commercial product.
In wheat bran medium described in the embodiment of the present invention, wheat bran is purchased from Qingdao wheat drifting fragrance flour Co., Ltd, remaining drug is purchased from state
Medicine group chemical reagent Beijing Co., Ltd.
In the present invention, the rhodotorula mucilaginosa solid pharmaceutical preparation improves peanut phosphorus nourishing situation, promotes peanut growth, reduces
Absorption of the peanut to Cd, while reducing Cd and being shifted from root system to seed, to reduce the Cd content in Ecological Property of Peanut Seeds.
Drop cadmium agent provided by the invention is it is also preferable to include the sulfosalisylic acid solution independently dispensed, in the present invention, the sulphur
The concentration of base salicylic acid solution is preferably 1~2g/L, more preferably 1.4~1.6g/L, most preferably 1.5g/L.In the present invention,
The sulfosalisylic acid solution can activate the defense mechanism of peanut to protect its own, and the poison that cadmium generates peanut is effectively relieved
Evil, while promoting the growth of peanut.In the present invention, the source of the sulfosalisylic acid solution is not particularly limited, using ability
Domain conventional commercial product.Chinese medicines group chemical reagent Beijing Co., Ltd is purchased from the embodiment of the present invention.
The present invention provides a kind of application of the drop cadmium agent described in above scheme in plantation peanut.
In the present invention, the proline solution seed soaking of 15~45mmol/L is preferably used before peanut cultivation.The proline
The concentration of solution is preferably 20~40mmol/L, more preferably 30mmol/L.The time of the seed soaking is preferably 4~6h, more excellent
It is selected as 4.5~5.5h, most preferably 5h.
In the present invention, seed resistance can be improved using proline solution seed soaking, and then improve the germination percentage of peanut seed.
In the present invention, the rhodotorula mucilaginosa fermentation liquid is applied in the rhizosphere of plantation peanut.The rhodotorula mucilaginosa fermentation liquid
Applied amount is preferably 16~20L/ mus, more preferably 17~19L/ mus, most preferably 18L/ mus.The rhodotorula mucilaginosa fermentation liquid
Applying number is 3 times.The rhodotorula mucilaginosa fermentation liquid is preferably diluted before administration, and the diluted multiple is preferably 100
Times.The time that the rhodotorula mucilaginosa fermentation liquid applies for the first time is preferably the 14~16d, more preferably 15d after peanut emergence.The
The time of secondary application is preferably 29~31d, more preferably 30d.The time that third time applies is preferably 44~46d, more preferably
For 45d.In the present invention, the rhodotorula mucilaginosa fermentation liquid preferably includes inactivation rhodotorula mucilaginosa fermentation liquid and does not inactivate rhodotorula mucilaginosa hair
Zymotic fluid does not inactivate rhodotorula mucilaginosa fermentation liquid more preferably.
In the present invention, the rhodotorula mucilaginosa solid pharmaceutical preparation is applied in the soil surface of plantation peanut.The rhodotorula mucilaginosa is solid
The each applied amount of body preparation is 8~10kg/ mus, more preferably 8.5~9.5kg/ mus, most preferably 9kg/ mus.The glue is red
The application time of yeast solids preparation is preferably 7~10d before peanut seeding, more preferably 8d.The side of heretofore described application
Formula is preferably spread fertilizer over the fields.In the present invention, solid pharmaceutical preparation that the rhodotorula mucilaginosa solid pharmaceutical preparation does not inactivate preferably.
In the present invention, the sulfosalisylic acid solution preferably peanut florescence spray plantation peanut blade face, every 9~
11d is sprayed once, is more preferably sprayed once every 10d.The number sprayed is preferably three times.The sulfosalisylic acid solution is every
The secondary amount of spraying is preferably 8~12L/ mus, more preferably 9~11L/ mus, most preferably 10L/ mus.In the present invention, the sulfo group
Salicylic acid solution is preferably diluted using preceding.The diluted multiple is preferably 50 times.
Below in conjunction with the embodiment in the present invention, the technical solution in the present invention is clearly and completely described.It is aobvious
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Embodiment 1
For trying soil:
Pick up from Qingdao City's Pingdu City contaminated soil (sandy loam), basic physical and chemical are as follows: pH 6.4 (Tu Shui is than 1:
2.5), organic matter 14.8g/kg, cation exchange capacity (CEC) 15.9cmol/kg, total cadmium content 3.05mg/kg.Pedotheque natural wind
It is dry, it is levigate to cross 2mm (10 mesh) sieve, for potting simulation test.(depth of cultivated soil is in 20cm or so in crop field, according to soil
Density (the 2.65g/m of earth3) and the depth of cultivated soil calculated, the weight of soil is 353t or so in field per acre.Root
The additive amount of cadmium agent drops in standard conversion pot experiment accordingly.All embodiments according to said method convert in the present invention)
For trying plant
Peanut (rich to spend No. 3) is purchased from Shandong Academy of Agricultural Sciences.
Pot experiment is implemented
It is packed into the soil sample 21.0kg for crossing 2mm sieve after air-drying in every basin, distilled water is added to water-retaining quantity among field of soil 16%, protects
It holds after soil moisture content balances 1 week and starts to sow.
Choose full seed, with volumetric concentration be 1% liquor natrii hypochloritis impregnate seed 10min after take out spend from
After sub- water cleans seed 5~7 times, it is put into the proline solution of 30mmol/L and impregnates 5h, dug in advance in the soil in basin
25 seeds are uniformly sowed in the consistent cave of depth.Then soil is covered on seed.Spray deionized water 50mL.It is measured after 7 days
Emergence rate.
Embodiment 2
For trying soil:
Pick up from Qingdao City's Pingdu City contaminated soil (sandy loam), basic physical and chemical are as follows: pH 6.4 (Tu Shui is than 1:
2.5), organic matter 14.8g/kg, cation exchange capacity (CEC) 15.9cmol/kg, total cadmium content 3.05mg/kg.Pedotheque natural wind
It is dry, it is levigate to cross 2mm (10 mesh) sieve, for potting simulation test.
For trying plant
Peanut (rich to spend No. 3) is purchased from Shandong Academy of Agricultural Sciences.
Pot experiment is implemented
It is packed into the soil sample 21.0kg for crossing 2mm sieve after air-drying in every basin, distilled water is added to water-retaining quantity among field of soil 16%, protects
It holds after soil moisture content balances 1 week and starts to sow.
Choose full seed, with volumetric concentration be 1% liquor natrii hypochloritis impregnate seed 10min after take out spend from
After sub- water cleans seed 5~7 times, it is put into the proline solution of 45mmol/L and impregnates 4h, dug in advance in the soil in basin
25 seeds are uniformly sowed in the consistent cave of depth.Then soil is covered on seed.Spray deionized water 20mL.It is measured after 7 days
Emergence rate.
Embodiment 3
For trying soil:
Pick up from Qingdao City's Pingdu City contaminated soil (sandy loam), basic physical and chemical are as follows: pH 6.4 (Tu Shui is than 1:
2.5), organic matter 14.8g/kg, cation exchange capacity (CEC) 15.9cmol/kg, total cadmium content 3.05mg/kg.Pedotheque natural wind
It is dry, it is levigate to cross 2mm (10 mesh) sieve, for potting simulation test.
For trying plant
Peanut (rich to spend No. 3) is purchased from Shandong Academy of Agricultural Sciences.
Pot experiment is implemented
It is packed into the soil sample 21.0kg for crossing 2mm sieve after air-drying in every basin, distilled water is added to water-retaining quantity among field of soil 16%, protects
It holds after soil moisture content balances 1 week and starts to sow.
Choose full seed, with volumetric concentration be 1% liquor natrii hypochloritis impregnate seed 10min after take out spend from
After sub- water cleans seed 5~7 times, it is put into the proline solution of 15mmol/L and impregnates 6h, dug in advance in the soil in basin
25 seeds are uniformly sowed in the consistent cave of depth.Then soil is covered on seed.Spray deionized water 20mL.It is measured after 7 days
Emergence rate.
Comparative example 1
In addition to not using proline solution seed soaking, other operating procedures are identical with embodiment 1.
Embodiment 4
Germination percentage in embodiment 1 and comparative example 1 is measured respectively.Concrete outcome is as shown in table 1.
The germination percentage of 1 different disposal group seed of table
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | |
| Emergence rate | 89% | 85% | 79% | 64% |
As can be seen from Table 1, emergence rate can be improved to seed-soaking using proline solution, compared with comparative example 1, hair
Bud rate improves 15%~25%.
Embodiment 5
For trying soil:
Pick up from Qingdao City's Pingdu City contaminated soil (sandy loam), basic physical and chemical are as follows: pH 6.4 (Tu Shui is than 1:
2.5), organic matter 14.8g/kg, cation exchange capacity (CEC) 15.9cmol/kg, total cadmium content 3.05mg/kg.Pedotheque natural wind
It is dry, it is levigate to cross 2mm (10 mesh) sieve, for potting simulation test.
For trying plant
Peanut (rich to spend No. 3) is purchased from Shandong Academy of Agricultural Sciences.
Strains tested
Rhodotorula mucilaginosa (Rhodotorula mucilaginosa) bacterial strain OP11 (deposit number: CGMCC No.13540),
Cell is round, oval or long rod.
Culture medium
Malt juice liquid medium (g/L): brewer's wort 20g, peptone 1g, glucose 20g, pH are natural.
Brewer's wort slant medium: brewer's wort 20g, peptone 1g, glucose 20g, pH are naturally, be settled to 1000mL.
The preparation of rhodotorula mucilaginosa bacterial strain fermentation liquid
By in rhodotorula mucilaginosa OP11 strain inoculated to brewer's wort slant medium at 30 DEG C after activation culture 48h, with connecing
In kind one ring of ring picking access malt juice liquid medium, 180r/min, 30 DEG C of culture 48h obtain rhodotorula mucilaginosa fermentation seed
Liquid.
Obtained rhodotorula mucilaginosa seed liquor is inoculated into malt juice liquid medium according to 4% inoculum concentration, at 28 DEG C,
Liquid fermentation 85h under conditions of revolving speed 180r/min obtains rhodotorula mucilaginosa fermentation liquid (the rhodotorula mucilaginosa zymotic fluid viable count
It is 6 × 108CFU/mL), and it is spare after 100 times of dilution.
Pot experiment is implemented
It is packed into the soil sample 21.0kg for crossing 2mm sieve after air-drying in every basin, distilled water is added to water-retaining quantity among field of soil 16%, protects
It holds after soil moisture content balances 1 week and starts to sow.
Choose full seed, with volumetric concentration be 1% liquor natrii hypochloritis impregnate seed 10min after take out spend from
After sub- water cleans seed 5~7 times, it is put into the proline solution of 30mmol/L and impregnates 5h, dug in advance in the soil in basin
6 seeds are uniformly sowed in the consistent cave of depth.Then soil is covered on seed.Spray deionized water 20mL.It is grown to plant
Thinning when 2 true leaves guarantees there are 3 peanuts in each basin.The 15d after peanut emergence, after every basin plant rhizosphere applies dilution
Rhodotorula mucilaginosa fermentation liquid 100mL.In planting process, keeping soil moisture content is 65% or so of maximum field capacity.
When cultivating 30d and 45d, respectively to the additional rhodotorula mucilaginosa fermentation liquid of plant rhizosphere, dosage is 100ml.After plant maturation, receive
Whole strain is obtained, plant is divided into overground part, underground part and pod with stainless steel scissors, measures plant fresh weight.It is rinsed with deionized water
Afterwards, 105 DEG C of water-removing 20min, 70 DEG C dry to constant weight, and crushing sieves with 100 mesh sieve.
Embodiment 6
For examination soil, for the preparation method of examination plant, strains tested, the method for medium treatment and rhodotorula mucilaginosa fermentation liquid
With embodiment 5.
Pot experiment is implemented
It is packed into the soil sample 21.0kg for crossing 2mm sieve after air-drying in every basin, distilled water is added to water-retaining quantity among field of soil 16%, protects
It holds after soil moisture content balances 1 week and starts to sow.
Choose full seed, with volumetric concentration be 1% liquor natrii hypochloritis impregnate seed 10min after take out spend from
After sub- water cleans seed 5~7 times, it is put into the proline solution of 30mmol/L and impregnates 5h, dug in advance in the soil in basin
6 seeds are uniformly sowed in the consistent cave of depth.Then soil is covered on seed.Spray deionized water 20mL.It is grown to plant
Thinning when 2 true leaves guarantees there are 3 peanuts in each basin.The 15d after peanut emergence, after every basin plant rhizosphere applies dilution
Rhodotorula mucilaginosa fermentation liquid 100mL.In planting process, keeping soil moisture content is 65% or so of maximum field capacity.
When cultivating 30d and 45d, respectively to the additional rhodotorula mucilaginosa fermentation liquid of plant rhizosphere, dosage is 100ml.It is opened simultaneously in peanut
Florescence to blade spraying concentration be 30mgL-1Sulfosalisylic acid solution, spray 30mL/ basin every time, spray within every 10 days it is primary,
Continuous spraying 3 times.After plant maturation, whole strain is harvested, plant is divided into overground part, underground part and pod with stainless steel scissors, is surveyed
It is colonized strain fresh weight.After being rinsed with deionized water, 105 DEG C of water-removing 20min, 70 DEG C dry to constant weight, and crushing sieves with 100 mesh sieve.
Embodiment 7
For examination soil, for examination plant, strains tested, medium treatment method with embodiment 5.
The preparation of rhodotorula mucilaginosa fermentation liquid
By in rhodotorula mucilaginosa OP11 strain inoculated to brewer's wort slant medium at 28 DEG C after activation culture 52h, with connecing
In kind one ring of ring picking access malt juice liquid medium, 170r/min, 31 DEG C of culture 44h obtain rhodotorula mucilaginosa fermentation seed
Liquid.
Obtained rhodotorula mucilaginosa seed liquor is inoculated into malt juice liquid medium according to 2.5% inoculum concentration, 30
DEG C, liquid fermentation 96h under conditions of revolving speed 150r/min, obtaining rhodotorula mucilaginosa fermentation liquid, (the rhodotorula mucilaginosa fermentation liquid is living
Bacterium number is 8 × 107CFU/mL), and it is spare after 100 times of dilution.
Pot experiment is implemented
It is packed into the soil sample 21.0kg for crossing 2mm sieve after air-drying in every basin, distilled water is added to water-retaining quantity among field of soil 16%, protects
It holds after soil moisture content balances 1 week and starts to sow.
Choose full seed, with volumetric concentration be 1% liquor natrii hypochloritis impregnate seed 10min after take out spend from
After sub- water cleans seed 5~7 times, it is put into the proline solution of 30mmol/L and impregnates 5h, dug in advance in the soil in basin
6 seeds are uniformly sowed in the consistent cave of depth.Then soil is covered on seed.Spray deionized water 20mL.It is grown to plant
Thinning when 2 true leaves guarantees there are 3 peanuts in each basin.The 14d after peanut emergence, after every basin plant rhizosphere applies dilution
Rhodotorula mucilaginosa fermentation liquid 95mL.In planting process, keeping soil moisture content is 65% or so of maximum field capacity.?
When cultivating 29d and 44d, respectively to the additional rhodotorula mucilaginosa fermentation liquid of plant rhizosphere, dosage is 95mL.Simultaneously in peanut florescence
It is 20mg.L to blade spraying concentration-1Sulfosalisylic acid solution, spray 36mL/ basin every time, spray within every 11 days it is primary, it is continuous to spray
It applies 3 times.After plant maturation, whole strain is harvested, plant is divided into overground part, underground part and pod with stainless steel scissors, measures plant
Fresh weight.After being rinsed with deionized water, 105 DEG C of water-removing 20min, 70 DEG C dry to constant weight, and crushing sieves with 100 mesh sieve.
Embodiment 8
For examination soil, for examination plant, strains tested, medium treatment method with embodiment 5.
The preparation of rhodotorula mucilaginosa fermentation liquid
By in rhodotorula mucilaginosa OP11 strain inoculated to brewer's wort slant medium at 32 DEG C after activation culture 36h, with connecing
In kind one ring of ring picking access malt juice liquid medium, 190r/min, 29 DEG C of culture 52h obtain rhodotorula mucilaginosa fermentation seed
Liquid.
Obtained rhodotorula mucilaginosa seed liquor is inoculated into malt juice liquid medium according to 5% inoculum concentration, at 25 DEG C,
Liquid fermentation 72h under conditions of revolving speed 200r/min obtains rhodotorula mucilaginosa fermentation liquid (the rhodotorula mucilaginosa zymotic fluid viable count
It is 1 × 108CFU/mL), and 100 times of dilutions are carried out.
Pot experiment is implemented
It is packed into the soil sample 21.0kg for crossing 2mm sieve after air-drying in every basin, distilled water is added to water-retaining quantity among field of soil 16%, protects
It holds after soil moisture content balances 1 week and starts to sow.
Choose full seed, with volumetric concentration be 1% liquor natrii hypochloritis impregnate seed 10min after take out spend from
After sub- water cleans seed 5~7 times, it is put into the proline solution of 30mmol/L and impregnates 5h, dug in advance in the soil in basin
6 seeds are uniformly sowed in the consistent cave of depth.Then soil is covered on seed.Spray deionized water 20mL.It is grown to plant
Thinning when 2 true leaves guarantees there are 3 peanuts in each basin.The 16d after peanut emergence, after every basin plant rhizosphere applies dilution
Rhodotorula mucilaginosa fermentation liquid 119mL.In planting process, keeping soil moisture content is 65% or so of maximum field capacity.
When cultivating 31d and 46d, respectively to the additional rhodotorula mucilaginosa fermentation liquid of plant rhizosphere, dosage is 119mL.It is opened simultaneously in peanut
Florescence to blade spraying concentration be 40mg.L-1Sulfosalisylic acid solution, spray 24mL/ basin every time, spray within every 9 days it is primary, even
It is continuous to spray 3 times.After plant maturation, whole strain is harvested, plant is divided into overground part, underground part and pod with stainless steel scissors, is measured
Plant fresh weight.After being rinsed with deionized water, 105 DEG C of water-removing 20min, 70 DEG C dry to constant weight, and crushing sieves with 100 mesh sieve.
Comparative example 2
For examination soil, for examination plant with embodiment 5, pot experiment embodiment in addition to not applying rhodotorula mucilaginosa fermentation liquid its
His operating procedure is identical with embodiment 5.
Comparative example 3
For examination soil, for examination plant, strains tested, culture medium and the preparation of rhodotorula mucilaginosa fermentation liquid method with embodiment 6,
Pot experiment embodiment other operating procedures in addition to not applying rhodotorula mucilaginosa fermentation liquid are identical with embodiment 6.
Comparative example 4
In addition to rhodotorula mucilaginosa fermentation liquid is the fermentation liquid of inactivation, other operating procedures are identical with embodiment 6.The red ferment of glue
Female fermentation liquid inactivation treatment mode are as follows: it takes rhodotorula mucilaginosa fermentation liquid that 6mol/L salt acid for adjusting pH is added to 2.5 and is heated to 90 DEG C,
Constant temperature 1h, then original pH is adjusted to 6mol/L sodium hydroxide solution.
Embodiment 9
Weight of root system, grain yield and P and Cd in Plant samples are measured in embodiment 5~8 and comparative example 2~4 respectively
Content, the wherein measuring method of P and Cd are as follows: use wet digestion (dense HNO3With HClO4Volume ratio is 4:1), ICP-MS measurement
P, Cd content, digestion and continuous mode in national standard substance (GBW07603GSV-2) be internal standard control sample analysis matter
Amount.Concrete outcome is as shown in tables 2 and 3.
Peanut Root System dry weight and grain yield under 2 different disposal of table
As can be seen from Table 2, rhodotorula mucilaginosa fermentation liquid provided by the invention can significantly improve weight of root system and seed produces
Amount, the rhodotorula mucilaginosa fermentation liquid not inactivated is more excellent compared to the effect after inactivation, and compared with comparative example 2, weight of root system is than comparison
Example 2 high 30.3%, grain yield is than comparative example 2 high 17.7%.Wherein using provided by the invention by rhodotorula mucilaginosa fermentation liquid and
Preparation effect made of sulfosalisylic acid solution is best.Compared with comparative example 2, weight of root system improves 35.77%~37.9%, seed
Grain yield is than comparative example 2 high 21.44%~22.2%.
Ecological Property of Peanut Seeds cadmium content and blade phosphorus content under 3 different disposal of table
As can be seen from Table 3, rhodotorula mucilaginosa fermentation liquid provided by the invention can significantly reduce Ecological Property of Peanut Seeds cadmium content, together
When dramatically increase blade P content, the rhodotorula mucilaginosa fermentation liquid not inactivated is more excellent compared to the effect after inactivation, with 2 phase of comparative example
Than Cd content low 29.869%, blade P content is high by 16.1%.Wherein using provided by the invention by rhodotorula mucilaginosa fermentation liquid and
Preparation effect made of sulfosalisylic acid solution is best.Compared with comparative example 2, Cd content low 41.79%~44.78%, blade P
Content is high by 19.30%~21.43%.
Embodiment 10
For trying soil
Pick up from Qingdao City Chengyang District contaminated soil (Shajiang black soil), basic physical and chemical are as follows: pH 7.11 (Tu Shui is than 1:
2.5), organic matter 17.9g/kg, cation exchange capacity (CEC) 15.1cmol/kg, total cadmium content 3.31mg/kg.Pedotheque natural wind
It is dry, it is levigate to cross 2mm (10 mesh) sieve, for potting simulation test.
For trying plant
Peanut (rich to spend No. 3) is purchased from Shandong Academy of Agricultural Sciences.
Strains tested
Rhodotorula mucilaginosa (Rhodotorula mucilaginosa) bacterial strain OP11 (deposit number: CGMCC No.13540),
Cell is round, oval or long rod.
Culture medium
Malt juice liquid medium (g/L): brewer's wort 20g, peptone 1g, glucose 20g, pH are natural.
Brewer's wort slant medium: brewer's wort 20g, peptone 1g, glucose 20g, pH are naturally, be settled to 1000mL.
The preparation of rhodotorula mucilaginosa solid pharmaceutical preparation
By in rhodotorula mucilaginosa OP11 strain inoculated to brewer's wort slant medium at 30 DEG C after activation culture 48h, with connecing
In kind one ring of ring picking access malt juice liquid medium, 180r/min, 30 DEG C of culture 48h obtain rhodotorula mucilaginosa fermentation seed
Liquid.Rhodotorula mucilaginosa fermentation seed liquid is inoculated into wheat bran medium solid fermentation 6d under the conditions of 28 DEG C by 8% inoculum concentration,
Stirring, obtains mixture；
The mixture that the step obtains is continued into the 5d that ferments at 30 DEG C, obtains rhodotorula mucilaginosa solid pharmaceutical preparation, it is described
Rhodotorula mucilaginosa solid pharmaceutical preparation viable count is 6 × 108CFU/g。
Pot experiment is implemented
It is packed into the soil sample 21.0kg for crossing 2mm sieve after air-drying in every basin, spreads fertilizer over the fields rhodotorula mucilaginosa solid pharmaceutical preparation in soil surface
0.5g is added distilled water to water-retaining quantity among field of soil 16%, starts to sow after keeping soil moisture content to balance 1 week.It chooses full
Seed be put into after taking-up cleans seed 5~7 times with deionized water after impregnating seed 10min with 1% liquor natrii hypochloritis
5h is impregnated in the proline solution of 30mmol/L, has dug the consistent cave of depth in the soil in basin in advance, uniformly sows 6 kinds
Then soil is covered on seed by son.Spray deionized water 20mL.Thinning when plant grows 2 true leaves.Guarantee every basin alms bowl 3
Peanut.After plant maturation, whole strain is harvested, plant is divided into overground part, underground part and pod with stainless steel scissors, measures plant
Fresh weight.After being rinsed with deionized water, 105 DEG C of water-removing 20min, 70 DEG C dry to constant weight, and crushing sieves with 100 mesh sieve.
Embodiment 11
For trying soil
Pick up from Qingdao City Chengyang District contaminated soil (Shajiang black soil), basic physical and chemical are as follows: pH 7.11 (Tu Shui is than 1:
2.5), organic matter 17.9g/kg, cation exchange capacity (CEC) 15.1cmol/kg, total cadmium content 3.31mg/kg.Pedotheque natural wind
It is dry, it is levigate to cross 2mm (10 mesh) sieve, for potting simulation test.
For the same implementation of preparation method of examination plant, strains tested, the method for medium treatment and rhodotorula mucilaginosa solid pharmaceutical preparation
Example 10.
Pot experiment is implemented
It is packed into the soil sample 21.0kg for crossing 2mm sieve after air-drying in every basin, spreads fertilizer over the fields rhodotorula mucilaginosa solid pharmaceutical preparation in soil surface
0.5g is added distilled water to water-retaining quantity among field of soil, starts to sow after keeping soil moisture content to balance 1 week.Choose full kind
Son is put into 30mmol/ after taking-up cleans seed 5~7 times with deionized water after impregnating seed 10min with 1% liquor natrii hypochloritis
5h is impregnated in the proline solution of L, has dug the consistent cave of depth in the soil in basin in advance, uniformly sows 6 seeds, then
Soil is covered on seed.Spray deionized water 20mL.Thinning when plant grows 2 true leaves.Guarantee 3 peanuts of every basin alms bowl.
In planting process, keeping soil moisture content is 65% or so of maximum field capacity.Simultaneously in peanut florescence to blade
Spraying concentration is 30mgL-1Sulfosalisylic acid solution, spray 30mL/ basin every time, spray within every 10 days primary, continuous spraying 3
It is secondary.After plant maturation, whole strain is harvested, plant is divided into overground part, underground part and pod with stainless steel scissors, measurement plant is fresh
Weight.After being rinsed with deionized water, 105 DEG C of water-removing 20min, 70 DEG C dry to constant weight, and crushing sieves with 100 mesh sieve.
Embodiment 12
For examination soil, for examination plant, strains tested, medium treatment method with embodiment 10.
The preparation of rhodotorula mucilaginosa solid pharmaceutical preparation
By in rhodotorula mucilaginosa OP11 strain inoculated to brewer's wort slant medium at 32 DEG C after activation culture 36h, with connecing
In kind one ring of ring picking access malt juice liquid medium, 190r/min, 29 DEG C of culture 52h obtain rhodotorula mucilaginosa fermentation seed
Liquid.Rhodotorula mucilaginosa fermentation seed liquid is inoculated into wheat bran medium solid fermentation under the conditions of 30 DEG C by 10% inoculum concentration
5d, stirring, obtains mixture；
The mixture that the step obtains is continued into the 3d that ferments at 30 DEG C, obtains rhodotorula mucilaginosa solid pharmaceutical preparation, it is described
Rhodotorula mucilaginosa solid pharmaceutical preparation viable count is 7 × 107CFU/g。
Pot experiment is implemented
It is packed into the soil sample 21.0kg for crossing 2mm sieve after air-drying in every basin, spreads fertilizer over the fields rhodotorula mucilaginosa solid pharmaceutical preparation in soil surface
0.41g is added distilled water to water-retaining quantity among field of soil, starts to sow after keeping soil moisture content to balance 1 week.Choose full kind
Son is put into 30mmol/ after taking-up cleans seed 5~7 times with deionized water after impregnating seed 10min with 1% liquor natrii hypochloritis
5h is impregnated in the proline solution of L, has dug the consistent cave of depth in the soil in basin in advance, uniformly sows 6 seeds, then
Soil is covered on seed.Spray deionized water 20mL.Thinning when plant grows 2 true leaves.Guarantee 3 peanuts of every basin alms bowl.
In planting process, keeping soil moisture content is 65% or so of maximum field capacity.Simultaneously in peanut florescence to blade
Spraying concentration is 20mgL-1Sulfosalisylic acid solution, spray 36mL/ basin every time, spray within every 9 days it is primary, continuous spraying 3 times.
After plant maturation, whole strain is harvested, plant is divided into overground part, underground part and pod with stainless steel scissors, measures plant fresh weight.With
After deionized water is rinsed, 105 DEG C of water-removing 20min, 70 DEG C dry to constant weight, and crushing sieves with 100 mesh sieve.
Embodiment 13
For examination soil, for examination plant, strains tested, medium treatment method with embodiment 10.
The preparation of rhodotorula mucilaginosa solid pharmaceutical preparation
It will be activated in the rhodotorula mucilaginosa OP11 strain inoculated to brewer's wort slant medium of laboratory preservation, 28 DEG C of trainings
52h is supported, is accessed in malt juice liquid medium with one ring of oese picking, 170r/min, 31 DEG C of culture 44h, the as red ferment of glue
Female fermentation seed liquid.Seed liquor is inoculated into wheat bran medium solid fermentation under the conditions of 30 DEG C according to the inoculum concentration by 10%
7d, stirring, obtains mixture；
The mixture that the step obtains is continued into the 5d that ferments at 30 DEG C, obtains rhodotorula mucilaginosa solid pharmaceutical preparation, it is described
Rhodotorula mucilaginosa solid pharmaceutical preparation viable count is 3 × 108CFU/g。
Pot experiment is implemented
It is packed into the soil sample 21.0kg for crossing 2mm sieve after air-drying in every basin, spreads fertilizer over the fields rhodotorula mucilaginosa solid pharmaceutical preparation in soil surface
0.54g is added distilled water to water-retaining quantity among field of soil 16%, starts to sow after keeping soil moisture content to balance 1 week.It chooses full
Seed be put into after taking-up cleans seed 5~7 times with deionized water after impregnating seed 10min with 1% liquor natrii hypochloritis
5h is impregnated in the proline solution of 30mmol/L, has dug the consistent cave of depth in the soil in basin in advance, uniformly sows 6 kinds
Then soil is covered on seed by son.Spray deionized water 20mL.Thinning when plant grows 2 true leaves.Guarantee every basin alms bowl 3
Peanut.In planting process, keeping soil moisture content is 65% or so of maximum field capacity.Simultaneously in peanut florescence
It is 40mgL to blade spraying concentration-1Sulfosalisylic acid solution, spray 24mL/ basin every time, spray within every 11 days it is primary, continuously
It sprays 3 times.After plant maturation, whole strain is harvested, plant is divided into overground part, underground part and pod with stainless steel scissors, measurement is planted
Strain fresh weight.After being rinsed with deionized water, 105 DEG C of water-removing 20min, 70 DEG C dry to constant weight, and crushing sieves with 100 mesh sieve.
Comparative example 5
For examination soil, for try plant, same to embodiment, pot experiment embodiment is not in addition to applying rhodotorula mucilaginosa solid pharmaceutical preparation
Other operating procedures are identical with embodiment 9.
Comparative example 6
For examination soil, for the same embodiment of method of examination plant, strains tested, culture medium and the preparation of rhodotorula mucilaginosa solid pharmaceutical preparation
10, pot experiment embodiment other operating procedures in addition to not applying rhodotorula mucilaginosa solid pharmaceutical preparation are identical with embodiment 10.
Comparative example 7
In addition to rhodotorula mucilaginosa solid pharmaceutical preparation is the solid pharmaceutical preparation of inactivation, other operating procedures and the complete phase of comparative example 10
Together.
Embodiment 14
Measure in embodiment 10~13 and comparative example 5~7 respectively in Plant samples weight of root system, grain yield and P and
The content of Cd, the wherein measuring method of P and Cd are as follows: use wet digestion (dense HNO3With HClO4Volume ratio is 4:1), ICP-MS is surveyed
Determine P, Cd content, with national standard substance (GBW07603GSV-2) is that internal standard controls sample analysis in digestion and continuous mode
Quality.Concrete outcome is as shown in table 4 and 5.
Peanut Root System dry weight and grain yield under 4 different disposal of table
As can be seen from Table 4, rhodotorula mucilaginosa fermentation liquid provided by the invention can significantly improve weight of root system and seed produces
Amount, the rhodotorula mucilaginosa fermentation liquid not inactivated is more excellent compared to the effect after inactivation, and compared with comparative example 5, weight of root system is than comparison
Example 5 high 20.58%, grain yield is than comparative example 5 high 17.2%.Wherein using provided by the invention by rhodotorula mucilaginosa fermentation liquid and
Preparation effect made of sulfosalisylic acid solution is best.Compared with comparative example 5, weight of root system improves 31.13%~32.7%, seed
Grain yield is than comparative example 5 high 20.85%~22%.
Ecological Property of Peanut Seeds cadmium content and blade phosphorus content under 5 different disposal of table
As can be seen from Table 5, rhodotorula mucilaginosa fermentation liquid provided by the invention can significantly reduce Ecological Property of Peanut Seeds cadmium content, together
When dramatically increase blade P content, the rhodotorula mucilaginosa fermentation liquid not inactivated is more excellent compared to the effect after inactivation, with 5 phase of comparative example
Than Cd content low 28.14%, blade P content is high by 14.55%.Wherein using provided by the invention by rhodotorula mucilaginosa fermentation liquid and
Preparation effect made of sulfosalisylic acid solution is best.Compared with comparative example 5, Cd content low 40.63%~43.75%, blade P
Content is high by 18.18%~20%.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (8)
- The application that cadmium drops in cadmium agent in plantation peanut drops 1. cultivating peanut, which is characterized in that the peanut drop cadmium agent includes that glue is red Yeast fermentation broth or rhodotorula mucilaginosa solid pharmaceutical preparation；The preparation method of the rhodotorula mucilaginosa fermentation liquid, comprising the following steps:Rhodotorula mucilaginosa bacterial strain is activated and expands culture, rhodotorula mucilaginosa bacterial strain fermentation seed liquid is obtained, by the Rhodotorula mucilaginose Strain fermentation seed liquid is inoculated into malt juice liquid medium by 2.5%~5% inoculum concentration, at 25~30 DEG C, revolving speed 150~ Liquid fermentation 72h~96h under conditions of 200r/min obtains rhodotorula mucilaginosa fermentation liquid；The preparation method of the rhodotorula mucilaginosa solid pharmaceutical preparation, comprising the following steps:A. rhodotorula mucilaginosa bacterial strain is activated and expands culture, rhodotorula mucilaginosa bacterial strain fermentation seed liquid is obtained, by the rhodotorula mucilaginosa Strain fermentation seed liquor is inoculated into wheat bran medium the solid fermentation 5 under the conditions of 25~30 DEG C by 5%~10% inoculum concentration ~7d, stirring, obtains mixture；B. the obtained mixture of the step a is continued at 28~30 DEG C 3~5d of fermentation, obtains rhodotorula mucilaginosa solid pharmaceutical preparation；The deposit number of the rhodotorula mucilaginosa bacterial strain is CGMCC No.13540.
- 2. applying according to claim 1, which is characterized in that the peanut drop cadmium agent further includes the mass concentration independently dispensed For the sulfosalisylic acid solution of 1~2g/L.
- 3. application according to claim 1, which is characterized in that use the proline of 15~45mmol/L before peanut cultivation Solution seed soaking.
- 4. application according to claim 1, which is characterized in that the rhodotorula mucilaginosa fermentation liquid sprays the root in plantation peanut Border；The applied amount of the rhodotorula mucilaginosa fermentation liquid is 16~20L/ mus.
- 5. application according to claim 1 or 4, which is characterized in that the application number of the rhodotorula mucilaginosa fermentation liquid is 3 It is secondary；The time applied for the first time is 14~16d after peanut emergence, second time for applying be after peanut emergence 29~ 31d, the time that third time applies are 44~46d after peanut emergence.
- 6. application according to claim 1, which is characterized in that the rhodotorula mucilaginosa solid pharmaceutical preparation is applied in plantation peanut Soil surface；The applied amount of the rhodotorula mucilaginosa solid pharmaceutical preparation is 8~10kg/ mus.
- 7. application according to claim 1 or 6, which is characterized in that the application number of the rhodotorula mucilaginosa solid pharmaceutical preparation is 1 Secondary, the time of application is 7~10d before peanut seeding.
- 8. application according to claim 1, which is characterized in that spray sulfosalisylic acid solution in peanut florescence and planting The blade face of peanut is planted, every 9~11d is sprayed once, and continuous spraying is three times；The each amount of spraying of the sulfosalisylic acid solution is 8 ~12L/ mus.
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