CN109761715A - A kind of Queensland nut special bio bacterial manure and preparation method thereof - Google Patents
A kind of Queensland nut special bio bacterial manure and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to plant planting fields, are related to a kind of bio-bacterial manure, and in particular to a kind of Queensland nut special bio bacterial manure and preparation method thereof.The bacterial manure is made from the following raw materials in parts by weight: 2-5 parts of microbial bacterial agent, 10-20 parts of 5-8 parts of 15-22 parts of 30-45 parts of organic fertilizer, nitrogenous fertilizer, phosphate fertilizer, the potash fertilizer of the green peel containing Queensland nut, trace element fertilizer 3-7 parts middle, 4-8 parts of humic acid, 1-3 parts of plant growth-promoting substance, 3-7 parts of bentonite, at 25 DEG C -30 DEG C of temperature and pH6.0-6.8, above-mentioned raw materials are mixed into screened and drying and are made.The bio-bacterial manure that the present invention announces can preferably promote Queensland nut fruit tree growth, and have inhibiting effect to weeds tree.The bio-bacterial manure can be applied in the production practices of Queensland nut cultivation.
Description
Technical field
The invention belongs to plant planting fields, are related to a kind of bio-bacterial manure, and in particular to a kind of Queensland nut special bio
Bacterial manure and preparation method thereof.
Background technique
Queensland nut (Macadamia integrifolia F.Muell) also known as macadamia originate in Australia,
Artificial cultivation history only more than 150 years, be a kind of emerging fruit tree.Start 60~seventies of 20th century of Queensland nut to introduce China.Closely
Nian Lai saves to form preliminary industry in Yunnan Province of China, especially after Dehong prefecture introducing and planting, large-scale planting, and will gradually at
For Dehong prefecture main economic forestry products.Mainly based on organic fertilizer, supplemented by chemical fertilizer, rarer Australia is hard for Queensland nut cultivation of fruit tree
The report of fruit special fertilizer.
A kind of Queensland nut special complex fertilizer of Chinese patent CN107805163A- and preparation method thereof and Chinese patent
A kind of Queensland nut compound fertilizer of CN107759323A- and preparation method thereof discloses a kind of dedicated fertilizer of Queensland nut, and two
Technical solution in part patent document takes more similar method to carry out fertilizer preparation: by organic domestic waste, animal wastes,
Queensland nut shell mixes decomposed formation organic fertilizer with zymophyte, then adds a great number of elements, matrix etc. again and is mixed to form Australia
Nut dedicated fertilizer.But following problems exist in the prior art: (1) organic fertilizer is providing the same of nutrition for Queensland nut fruit tree
When, it can also promote weed growth between tree；(2) above-mentioned the comprehensive of organic fertilizer nutrition is lacking；(3) organic fertilizer may be bred greatly
Disinfect pathogen is measured, Queensland nut fruit diseases are caused；(4) above-mentioned technical proposal prepares fertilizer using Queensland nut shell fermentation,
The cellulose and lignin of shell are more difficult to be fermented decomposition, and nutrient utilization is not high.
Summary of the invention
The invention mainly solves the technical problem of providing a kind of Queensland nut special bio bacterial manure, bio-bacterial manure is for Australia
While continent nut fruit tree growth provides nutrient, weed growth between tree can inhibit.
In order to achieve the above objectives, the invention proposes following technical schemes:
A kind of Queensland nut special bio bacterial manure, is made from the following raw materials in parts by weight: 2-5 parts of microbial bacterial agent contains
10-20 parts of 5-8 parts of 15-22 parts of 30-45 parts of organic fertilizer, nitrogenous fertilizer, phosphate fertilizer, the potash fertilizer of Queensland nut green peel, middle trace element fertilizer 3-7
Part, 4-8 parts of humic acid, 1-3 parts of plant growth-promoting substance and 3-7 parts of bentonite.
It by adopting the above technical scheme, include various needed for Queensland nut growth and development of fruit tree in Queensland nut dedicated fertilizer
Nutriment and plant growth regulating substance, various elements act synergistically and realize the promotion to growth and development of fruit tree.Microorganism
The addition of microbial inoculum being capable of fruit diseases caused by pre- anti-fungal or bacterium etc..The organic fertilizer of the green peel containing Queensland nut is Queensland nut
Fruit tree growth provides nutrient, and inhibits weed growth between tree simultaneously, to improve utilization rate of fertilizer.
Beneficial effects of the present invention are specific as follows:
Queensland nut fruit tree growth fast speed, for example, the tree of triennial, set it is high up to 3m, hat width up to 2.5m ×
2.5m.And Queensland nut category shallow root system crop, main root is undeveloped, based on lateral root, the shallow narrow range of Root Distribution.So as to cause
Queensland nut fruit tree root system absorbs nutrient deficiency and the fast branch of aerial forms contradiction, need to increase the applied amount of fertilizer with
Meet the growth demand of aerial part.In the prior art, be the nutritional need for meeting fruit tree growth He yielding positive results, it is a large amount of and
Application of organic fertilizers in larger range, the use of organic fertilizer are not only that fruit tree provides nutriment, also promote weeds in orchard
Growth.Due to the feature that Queensland nut tree crown is big and Root Distribution is wide, need to retain wider tree spacing when being colonized treelet.Weeds
Using the nutrient in fertilizer between tree raised growth, since spacing is big, the growing space of weeds also becomes larger therewith, to Queensland nut
Nutrient competition effect it is stronger, greatly reduce fertilising efficiency.It is followed by that the nutrient growth of nut treelet is pressed down
The phenomenon that system, cannot form sufficiently large result tree crown, lack nutrition at tree reproductive growth, generate a large amount of fruit drops.For solution
The certainly above problem, in the prior art using artificial periodically the methods of weeding or herbicide, but the above method increases labor intensity,
And it is likely to result in environmental pollution, furthermore herbicide itself, which can also grow Queensland nut, generates certain negative effect.
The technical program use the green peel containing Queensland nut organic fertilizer as Queensland nut special bio bacterial manure at divide it
One, above-mentioned technical problem can be solved.It is studied through inventor and finds that effective component is to common weed in orchard in Queensland nut green peel
With growth inhibition effect, but does not have inhibition influence to Queensland nut fruit tree itself and nutrients can be provided for fruit tree growth
Matter, therefore Queensland nut green peel is used in the preparation of organic fertilizer.Green peel contains protein and total reducing sugar more abundant etc., containing blueness
The organic fertilizer of skin can provide nutrient for fruit tree growth.Organic fertilizer using the green peel containing Queensland nut not only can be fruit
Tree provides necessary nutriment, can also inhibit weed growth, so as to avoid weeds to fruit tree in terms of nutriment
Competitive inhibitory effect.Green peel is not found both for the nutriment in green peel to the comprehensive utilization of green peel in the prior art
Other purposes.In the present invention, inventor has found in green peel containing the ingredient for inhibiting weeds, and green peel is added in fertilizer
In, it can be achieved that inhibit weeds and provide nutrition double action, provide new direction for the comprehensive utilization of green peel.
In the prior art, cultivating fertilizer used in Queensland nut fruit tree is farm organic fertilizer mostly, using bagasse, vegetables
The organic domestic wastes mixing animal wastes such as slag and zymophyte, through a long time fermentation are made.But it exists in the prior art
The not comprehensive nutrition of machine fertilizer, nutritional ingredient problem not easy to control.Queensland nut dedicated fertilizer provided by the invention is in organic fertilizer
The substances such as a great number of elements, middle microelement are added on the basis of material can increase the comprehensive of Fertilizer nutrient, and nutritional ingredient is more
It is controllable.
Farmyard manure in the prior art, fertilizer efficiency is slow, and effect is poor in terms of promoting plant growth, lacks plant growth regulating
The addition of substance.The Queensland nut dedicated fertilizer that the technical program provides is added to plant growth-promoting substance, and plant growth-promoting substance is made
It for a kind of plant growth regulating substance, is absorbed by fruit tree root system, promotes the growth and development of fruit tree.
In the prior art, cultivating fertilizer used in Queensland nut fruit tree is farm organic fertilizer mostly, and there are fertilizer for organic fertilizer
In the problem of easily breeding various harmful microorganisms, cause fruit tree disease easily occur, eventually leading to fruit tree survival rate reduces or solid
The consequences such as rate reduction.Microbial bacterial agent can pernicious bacteria in antagonism soil, prevent Queensland nut fruit tree root lesion, and reduce agriculture
Reduce environmental pollution with the use of inhibiting-bacteria preparation.
In conclusion the invention has the benefit that
(1) using the organic fertilizer of the green peel containing Queensland nut, nutriment is being provided for Queensland nut fruit tree growth and result
While, it is suppressed that it is right in terms of nutriment to reduce weeds to ensure that utilization rate of fertilizer for the growth of weeds in orchard
The competitive inhibitory effect of fruit tree；
(2) the comprehensive of Fertilizer nutrient can be increased by being added to the substances such as a great number of elements, middle microelement, and nutritional ingredient is more
It is controllable；
(3) it is added to plant growth-promoting substance, coordinate plant growth promotes the growth and development of fruit tree；
(4) joined microbial bacterial agent, can pernicious bacteria in antagonism soil, prevent Queensland nut fruit tree root lesion, and
The use for reducing agricultural inhibiting-bacteria preparation reduces environmental pollution.
Further, the organic fertilizer of the green peel containing Queensland nut is made from the following raw materials in parts by weight: 5 parts of dish leaf, Australia
5 parts of nut green peel, 5 parts of animal wastes, 1 part of dregs of beans, 0.5 part of vinasse and 0.5 part of edible fungi residue.
By adopting the above technical scheme, Queensland nut green peel is the part abandoned in nut process, and the technical program will
Green peel is comprehensively utilized, and is reduced environmental pollution.
In the prior art, mostly use Queensland nut shell as the chief component of Queensland nut organic fertilizer greatly, but
The main component for being Queensland nut shell is cellulose, lignin etc., only can provide the elements such as C, H, O, ingredient is more single.And
And also complete decomposition relatively difficult to achieve, nutrient utilization rate are low by fermentation for cellulose and lignin.Technical solution of the present invention
The middle raw material for having used Queensland nut green peel as organic fertilizer fermentation, green peel contain protein and total reducing sugar more abundant
Deng realizing the comprehensive utilization of waste.
Further, the organic fertilizer of the green peel containing Queensland nut comprises the following steps: by dish leaf, Queensland nut green peel,
Animal wastes, dregs of beans, vinasse, edible fungi residue mix in proportion, and EM bacteria agent is added, and adjusting moisture content is 30-35 weight %,
Adjusting pH value be 6.5-7.5, decomposed 30-45 days to get.
By adopting the above technical scheme, it is decomposed through nutriments such as albumen, carbohydrates in everfermentation organic domestic waste, for
Fruit tree root system absorbs.Wherein inhibit ingredient containing plant growth in Queensland nut green peel, fermented rear inhibiting effect reduces, to fruit
Tree does not generate growth inhibition effect, but has a degree of inhibiting effect to the sprouting of herbal seed, growth.This technology
The organic fertilizer that scheme uses is different from the organic fertilizer of the prior art, and only fruit tree does not provide nutriment, can also inhibit week
The Competitive assays that enclosure planting object generates fruit tree in terms of nutrition.
Further, the middle trace element fertilizer is made from the following raw materials in parts by weight: 1-2 parts of Chelating state zinc, Chelating state manganese
1-2 parts, 3-5 parts of boron, 1-2 parts of sulphur, 1-2 parts of magnesium, 2-5 parts of calcium, 1-2 parts of iron, 0.5-1 parts of molybdenum.
By adopting the above technical scheme, middle microelement needed for Queensland nut fruit tree growth is provided, guarantees nut setting percentage.
Zinc, manganese, boron synergistic effect, promote Chlorophyll synthesis, increase leaf photosynthesis efficiency, promote the synthesis of organic matter and organic
Transhipment of the substance to fruit position, to promote Fruit and the wherein synthesis of sucrose, reduced sugar, lipid and protein.
Further, the nitrogenous fertilizer be any one of ammonium fertilizer, nitrate fertilizer, amide nitrogen fertilizer and organic nitrogenous fertilizer or
Any two or two or more combinations；The phosphate fertilizer is phosphorus pentoxide；The potash fertilizer is in potassium oxide, potassium sulfate and potassium chloride
Any or any two or two or more combinations.
By adopting the above technical scheme, the addition supplement of nitrogenous fertilizer, phosphate fertilizer and potash fertilizer can solve organic fertilizer fertility deficiency, battalion
Support incomplete problem.
Further, the water content of the humic acid is 3-5 weight %, and the content of organic matter of the humic acid is greater than or equal to
75 weight %.
By adopting the above technical scheme, humic acid can provide organic nitrogen, the nitrogen synergistic effect of different shape, fertilizer in fertilizer
Effect is fast, is conducive to absorption of the root system to nitrogen.
Further, the plant growth-promoting substance is made from the following raw materials in parts by weight: 1-2 parts of chitosan, amino acid 1-2
Part, 0.1-0.2 parts of seaweeds, 0.2-0.5 parts of root-inducing powder.
By adopting the above technical scheme, chitosan can be used as skeleton Absorption Growth promotion substance, so that reducing growth promotes object
The loss of matter increases the action time of plant growth-promoting substance, to reduce spraying times.And chitosan, amino acid, seaweed
Element, root-inducing powder can promote plant growth, and four synergistic effects can reinforce Plant growth-promoting effect effect.
Chitosan is the deacetylated product of chitin, is a kind of large biological molecule of chain structure, has good machinery
Performance and stable chemical property, it is a kind of environment-friendly polymers that abundance, low in cost, preparation is simple.Shell
Containing a large amount of free amino and hydroxyl in the molecular structure of glycan, chitosan has polycation, chelating, easily film forming, pH quick
The properties such as sense, humidity sensitive and higher chemical activity.The free amino and hydroxyl of chitosan can be with other material molecule shapes
At non-covalent bonds such as hydrogen bonds, active principle can be made to be attached to around chitosan molecule, realize the slow releasing function of active principle, it can
Realize the absorption to growth helping matters such as amino acid, seaweeds and root-inducing powders.In addition, chitosan itself can be used as plant life
Long regulator is adjusted Endogenous Hormones, influences the synthesis of phenols and lignin.Chitosan also can induce the anti-of Queensland nut
Sick resistance.
Amino acid is present in fertilizer as the smallest molecule for constituting protein, has and is easy to the characteristics of being absorbed by crops.But
It is that most of fertilizer amino acid is soluble easily in water, cannot retains near root system for a long time after application.Amino acid is frequently as blade face
The raw material of fertilizer, and amino acid is applied to root system, there are the above problems.The technical program adsorbs amino acid using chitosan, so that
Rate of ultilization of amino acid greatly improves.
Seaweeds are pure natural marine algae extracts, contain nutritional ingredient and natural mineral matter, growth regulator necessary to plant,
It can promote plant cell division and elongation, strengthen metabolism, accelerate root development, improve absorption of the plant to moisture and nutrient
Ability enhances anti-adversity ability, improves crop quality, improves yield.
The effect of root-inducing powder is the apical dominance that plant is maintained in plant, the photosynthetic assimilation products of inducer blade
It is transported to product (fruit), promotes plant establishment.
Further, the microbial bacterial agent includes the raw material bacterial strain of following parts by weight: 2-3 parts of bacillus licheniformis, huge
1-2 parts of bacillus, 1-2 parts of bacillus pumilus, 2-3 parts of bacillus cereus and 3-5 parts of colloid bacillus cereus.
By adopting the above technical scheme, technical principle are as follows: there is synergistic effect between mentioned microorganism, produced by secondary metabolism
The mechanism such as secretion, fermentation and the competitive inhibitory effect of object, microbial bacterial agent acts on Queensland nut fruit tree and fruit tree is raw
Long soil environment, induction fruit tree root system are grown to deep subsoil, improve soil microenvironment, inhibit disinfect pathogen in soil
Breeding, decompose soil in organic matter for fruit tree absorb.Microbial bacterial agent is promoted the growth of fruit tree, is mentioned by comprehensive function
High fruit yield.
Wherein, the deposit number for the bacillus licheniformis (Bacillus licheniformis) that this programme uses are as follows:14580D-5TM, the strain is purchased from American Type Culture collection warehousing (American type culture
Collection, ATCC), it is bacillus licheniformis type culture (Bacillus licheniformis (Weigmann)
Chester)；
The deposit number for the bacillus megaterium (Bacillus megaterium) that this programme uses are as follows:
14581TM, which is purchased from ATCC, for bacillus megaterium type culture (Bacillus megaterium de Bary)；
The deposit number for the bacillus pumilus (Bacillus pumilus) that this programme uses are as follows:7061TM, should
Strain is purchased from ATCC, is bacillus pumilus type culture (Bacillus pumilus Meyer and Gottheil)；
The deposit number for the bacillus cereus (Bacillus cereus) that this programme uses are as follows:14579D-
5TM, it is bacillus cereus type culture (Bacillus cereus Frankland and which, which is purchased from ATCC,
Frankland)；
The deposit number for the colloid bacillus cereus (Bacillus mucilaginosus) that this programme uses are as follows:
10792TM, it is colloid bacillus cereus type culture (Bacillus mucilaginosus which, which is purchased from ATCC,
Berliner)。
The beneficial effect of the technical program is specific as follows:
(1) stimulation Queensland nut fruit tree main root increases, so that root system is grown to deep subsoil
Auxin secretion, transport and the distribution of the controllable Queensland nut fruit tree root of the bacteria agent composition of this programme,
To stimulate fruit tree root to grow to deep subsoil, fruit tree is set to absorb the moisture and nutrient of deep subsoil, simultaneously
Since the depth of root is pricked, the wind loading rating of fruit tree is further strengthened, to improve the ability that fruit tree adapts to environment.
Queensland nut fruit tree main root is undeveloped, and main root system is distributed in soil shallow layer.At the florescence in spring of arid, fruit tree
Moisture and nutrient can not be obtained from deep soil for growth of young；Since Root Distribution is shallow, wind resistance is weak for fruit tree, encounters strong
Wind can be such that adult fruit tree covers, to cause production loss.The prior art, which is generally used, applies plant growth regulating into soil
The method of substance, to stimulate fruit tree root growth, such as exogenous auxin, gibberellin.The scheme of the prior art is lacked there are various
Point.Disadvantage one is excessive to lateral root growth stimulation.Growth regulatory substance promotes the development of lateral root and main root simultaneously, due to side
Radical amount is more and distribution is shallower, and the inducing action that growth regulatory substance compares main root to the inducing action of lateral root is stronger, this is just
Main root is caused to increase few, but lateral root raised growth.In the limited orchard in space, due to the raised growth of lateral root, fruit tree
It influences each other between lateral root, the common nutriment for competing shallow-layer, also resulting in that fruit tree nutrition is bad or dose increases etc. asks
Topic.Root system largely develops in soil shallow-layer, and certain difficulty can be also caused to ditching-fertilizing because trench digging need to avoid root it is intensive
Region to reduce the damage of mechanism and the excessive fertilizer of concentration to root.Disadvantage two is to have to main root growth stimulation
Limit.Main root increment can not enough solve Queensland nut fruit tree wind loading rating difference very well and cannot make full use of deep subsoil
The problem of nutrition.Disadvantage three is that action time is too short.Although growth regulatory substance can snap action, acting duration compared with
It is short, it needs repeatedly to apply often application, is just able to maintain its stimulation to root growth.Disadvantage four is environmental pollution.Excessive
Can also ecological environment be caused centainly to negatively affect by applying artificial growth regulatory substance.
The technical program can solve more than technical problem.The microbial bacterial agent composition of this programme may act on Australia
Nut fruit tree endogenous growth hormone synthesis secretion access, can be synthetically produced slight inhibiting effect to endogenous growth hormone.Plant
Root is more sensitive to growth hormone, and the growth hormone of low concentration can promote the growth of root, the growth hormone pair of higher concentration
The growth of root shows as inhibiting effect.The growth hormone of the cell of tip of a root root cap area and meristematic zone synthesis, under gravity
Transport lower part in position into root system, causes the growth hormone accumulated in main root meristematic zone and elongation zone more, right
The growth of main root produces inhibiting effect, and amount of the growth hormone at lateral root position is less, is just suitble to the growth of lateral root.This programme
Microbial bacterial agent composition slightly inhibit the synthesis of Endogenous auxin so that the auxin that accumulates in main root is reduced, generate
Certain promotion main root growth.It being reduced since endogenous growth hormone synthesizes, the level of growth hormone of lateral root part reduces,
Growth hormone also decreases the stimulation of lateral root, and the level of growth of lateral root is controlled in a certain range.
(2) mitigate disease, reduce pesticide residue
Various bacillus, which breed rapidly, after microbial bacterial agent composition is manured into soil, in this composition becomes dominant bacteria,
The nutrition and other resources for controlling root system, cause pathogen to lose living space and condition.Various gemma bars in this composition
The secondary metabolite of bacterium improves fruit tree epiblem cell wall thickening, fibrosis, degree of lignification, strengthens the diseases prevention of fruit tree
Harmful ability.The prior art handles the fruit diseases of Queensland nut usually using pesticide, has to environment a degree of broken
Bad effect, and pesticide can also stimulate harmful microorganism to generate drug resistance.The technical program overcomes the defect of the prior art,
Has the function of biological control.
(3) fertilizer efficiency is improved
The bacteria agent composition of this programme can decompose the organic matter in soil or fertilizer, and the organic matter after decomposition is conducive to
Fruit tree absorbs, the utilization rate of increased fertilizer or nutriment.The microbial bacterial agent composition of this programme can also synthesize secretion
The nutriments such as unavailable phosphorus, calcium, magnesium are converted into available state, further mentioned by a variety of organic acids, enzyme, physiological activator etc.
High utilization of nutrients.
Further, the microbial bacterial agent comprises the following steps:
Step a: by bacillus licheniformis, bacillus megaterium, bacillus pumilus, bacillus cereus and colloid gemma
Bacillus is expanded respectively connects big cultivation and fermentation, obtains 5 kinds of zymocyte liquids；
Step b: separation obtains 5 kinds of thallus respectively from 5 kinds of zymocyte liquids in step a, and 5 kinds of thallus are abundant in proportion
It mixes, obtains Mixed Microbes；
Step c: the Mixed Microbes in step b are expanded culture, mixed bacteria liquid is obtained；
Step d: separation obtains mixing thallus from the mixed bacteria liquid in step c, by mixing thallus drying to get described micro-
Bacteria agent composition.
By adopting the above technical scheme, by 5 kinds of single bacteriums carry out expand connect cultivation and fermentation after so that 5 kinds of bacillus numbers and work
Property increase；Then according to the ratio of 2-3:1-2:1-2:2-3:3-5 (mass ratio) by bacillus licheniformis, bacillus megaterium,
Bacillus pumilus, bacillus cereus and the mixing of colloid bacillus cereus thallus；Mixed bacillus connects culture by expansion
Microbial bacterial agent composition of the invention can be made with drying.
Further, a kind of preparation method of Queensland nut special bio bacterial manure, by regulation proportion by microbial bacterial agent, organic
Fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, middle trace element fertilizer, humic acid, plant growth-promoting substance, bentonite are thoroughly mixed uniformly；?
At 25-30 DEG C of temperature and pH 6.0-6.8, screened and drying is made.
By adopting the above technical scheme, raw material is dried to the bio-bacterial manure that this programme can be made after mixing, after dry
Bacterial manure is easily stored and transports.
Detailed description of the invention
Fig. 1 is the histogram of 1 data of experimental example: carrying out crop field using the fertilizer prepared in embodiment 1-3 and comparative example 1-4
Randomized block experiment monitors the variation of Queensland nut seedling leaves number (piece) under Different Fertilization；
Fig. 2 is the histogram of 1 data of experimental example: carrying out crop field using the fertilizer prepared in embodiment 1-3 and comparative example 1-4
Randomized block experiment monitors the variation of Queensland nut seedling leaf fresh weight (g) under Different Fertilization；
Fig. 3 is the histogram of 1 data of experimental example: carrying out crop field using the fertilizer prepared in embodiment 1-3 and comparative example 1-4
Randomized block experiment monitors the variation of Queensland nut seedling main root long (cm) under Different Fertilization；
Fig. 4 is the histogram of 1 data of experimental example: carrying out crop field using the fertilizer prepared in embodiment 1-3 and comparative example 1-4
Randomized block experiment monitors the variation of Queensland nut seedling root fresh weight (g) under Different Fertilization；
Fig. 5 is the histogram of 2 data of experimental example: with Queensland nut green peel dichloromethane extract to woodland common weed
Seed is handled, allelopathy of the monitoring extract to weeds.
Fig. 6 is the experimental result histogram of experimental example 3: illustrating the microbial bacterial agent composition of the technical program to Australia
The influence of nut root system endogenous growth cellulose content (* is indicated: there were significant differences compared with blank control, P < 0.05).
Specific embodiment
It is further described below by specific embodiment, in which:
Embodiment 1-3 is the preparating example of Queensland nut special bio bacterial manure；Comparative example 1 is farmyard manure in the prior art
Preparating example；Comparative example 2-4 is that omitting elements in embodiment 1-3 (are omitted green peel, chitosan and microbial bacteria respectively respectively
Agent) fertilizer preparating example；Experimental example 1 is to carry out the random area in crop field using the fertilizer prepared in embodiment 1-3 and comparative example 1-4
Group test；Experimental example 2 is that the allelopathy of Queensland nut peel extract is tested；Experimental example 3 is microbial bacterial agent composition pair
The influence of Queensland nut root system endogenous growth cellulose content；Experimental example 4 is microbial bacterial agent composition to Queensland nut root growth
The influence of development.
Embodiment 1: the fat example of Queensland nut special bacterium is prepared
(1) raw material preparation and preparation
(1.1) containing the fertilizer of a great number of elements
Nitrogenous fertilizer: selecting ammonium fertilizer and nitrate fertilizer to mix according to mass ratio 2:1, and ammonium fertilizer uses ammonium hydrogen carbonate to live
Property ingredient nitrogenous fertilizer (green river biology), nitrate fertilizer use sodium nitrate for the nitrogenous fertilizer of active constituent (green river is biological).
Phosphate fertilizer: use phosphorus pentoxide for the phosphate fertilizer (the Henan right way) of active constituent.
Potash fertilizer: use potassium sulfate for the potash fertilizer (western Shandong chemical fertilizer) of active constituent.
(1.2) trace element fertilizer in: middle trace element fertilizer is self-control, and each middle microelement is final concentration of: Chelating state zinc 2g/
L, Chelating state manganese 2g/L, boron 5g/L, sulphur 2g/L, magnesium 2g/L, calcium 2g/L, iron 1g/L, molybdenum 1g/L.
(1.3) humic acid (Chengdu lid Er Gaisi), is detected 4.1 weight % of water content, and the content of organic matter is 82 weight %.
(1.4) plant growth-promoting substance
By chitosan (Chengdu lid Er Gaisi) 2kg, amino acid fertilizer (deep logical fertile industry) 2kg, seaweeds (Qingdao Ostwald-Folin is grand)
0.1kg, root-inducing powder (Shandong Liangshan ancient cooking vessel is holy) 0.4kg mixing, are made plant growth-promoting mixture of substances.
(1.5) microbial bacterial agent:
Microbial bacterial agent includes bacillus licheniformis (A), bacillus megaterium (B), bacillus pumilus (C), waxy bud
Spore bacillus (D), colloid bacillus cereus (E)；Its production method is after 5 kinds of bacterial strains expand connect cultivation and fermentation respectively, and bacterium solution is with sterile
Solid absorbent absorption, after mixing well by weight A:B:C:D:E=3:1:2:3:5,25 DEG C of constant temperature stand dark culture 2 days,
Then it is dried under the conditions of 30 DEG C, moisture content is 30 weight % to get microbial bacterial agent.
(1.6) bentonite (Sichuan and prosperous profit reach).
(1.7) organic fertilizer
Dish leaf 5kg, Queensland nut green peel 5kg, animal wastes 5kg, dregs of beans 1kg, vinasse 0.5kg, edible fungi residue 0.5kg
The EM bacteria agent (Shandong organic fertilizer factory) of final concentration of 2 weight % is added in mixing, and adjusting moisture content is 30 weight %, adjusts
PH value is 7.0, and decomposed 40 days to get the organic fertilizer.
(2) prepared by Queensland nut special bio bacterial manure
Take microbial bacterial agent 2kg, organic fertilizer 40kg, nitrogenous fertilizer 19kg, phosphate fertilizer 6kg, potash fertilizer 17kg, middle trace element fertilizer 5kg,
Humic acid 6kg, plant growth-promoting substance 2kg, bentonite 5kg, above-mentioned substance is thoroughly mixed uniformly；At 25 DEG C -30 of temperature
DEG C, under the conditions of pH 6.0-6.8, Queensland nut special bio bacterial manure is made in screened and drying.
(3) Queensland nut special bio bacterial manure technical indicator
It is carried out according to Queensland nut special bio bacterial manure obtained in the present embodiment of agriculture ministerial standard NY/T798-2015 pair
Detection, as a result as follows:
(3.1) appearance: product appearance is graininess, without obvious mechanical admixture, uniform in size.
(3.2) technical indicator is shown in Table 1:
1 Queensland nut special bio bacterial manure technical indicator (embodiment 1) of table
| Project | Testing result |
| Living bacteria count (cfu), hundred million/g | It is qualified |
| Total nutrient (N+P2O5+K2O), % | It is qualified |
| Organic matter (to dry in terms of base), % | It is qualified |
| Miscellaneous bacteria rate, % | It is qualified |
| Moisture, % | It is qualified |
| PH value | It is qualified |
(3.3) innoxious index, is shown in Table 2:
The 2 innoxious index of Queensland nut special bio bacterial manure (embodiment 1) of table
Embodiment 2: the fat example of Queensland nut special bacterium is prepared
(1) raw material preparation and preparation
(1.1) containing the fertilizer of a great number of elements
Nitrogenous fertilizer: selecting ammonium fertilizer, and ammonium fertilizer uses ammonium hydrogen carbonate for the nitrogenous fertilizer (green river biology) of active constituent.
Phosphate fertilizer: use phosphorus pentoxide for the phosphate fertilizer (the Henan right way) of active constituent.
Potash fertilizer: use potassium oxide for the potash fertilizer (western Shandong chemical fertilizer) of active constituent.
(1.2) trace element fertilizer in: middle trace element fertilizer is self-control, and each middle microelement is final concentration of: Chelating state zinc 1g/
L, Chelating state manganese 1g/L, boron 4g/L, sulphur 2g/L, magnesium 2g/L, calcium 5g/L, iron 1g/L, molybdenum 0.5g/L.
(1.3) humic acid (Chengdu lid Er Gaisi), is detected 4.1 weight % of water content, and the content of organic matter is 82 weight %.
(1.4) plant growth-promoting substance
By chitosan (Chengdu lid Er Gaisi) 2kg, amino acid fertilizer (deep logical fertile industry) 1kg, seaweeds (Qingdao Ostwald-Folin is grand)
0.2kg, root-inducing powder (Shandong Liangshan ancient cooking vessel is holy) 0.2kg mixing, are made plant growth-promoting mixture of substances.
(1.5) microbial bacterial agent:
Microbial bacterial agent includes bacillus licheniformis (A), bacillus megaterium (B), bacillus pumilus (C), waxy bud
Spore bacillus (D), colloid bacillus cereus (E)；Its production method is after 5 kinds of bacterial strains expand connect cultivation and fermentation respectively, and bacterium solution is with sterile
Solid absorbent absorption, after mixing well by weight A:B:C:D:E=2:2:2:2:3,25 DEG C of constant temperature stand dark culture 2 days,
Then it is dried under the conditions of 30 DEG C, moisture content is within 28 weight % to get microbial bacterial agent.
(1.6) bentonite (Sichuan and prosperous profit reach).
(1.7) organic fertilizer
Dish leaf 5kg, Queensland nut green peel 5kg, animal wastes 5kg, dregs of beans 1kg, vinasse 0.5kg, edible fungi residue 0.5kg
The EM bacteria agent (Shandong organic fertilizer factory) of final concentration of 1 weight % is added in mixing, and adjusting moisture content is 35 weight %, adjusts
PH value is 7.0, and decomposed 45 days to get the organic fertilizer.
(2) prepared by Queensland nut special bio bacterial manure
Take microbial bacterial agent 5kg, organic fertilizer 45kg, nitrogenous fertilizer 22kg, phosphate fertilizer 8kg, potash fertilizer 20kg, middle trace element fertilizer 7kg,
Humic acid 8kg, plant growth-promoting substance 3kg, bentonite 7kg, above-mentioned substance is thoroughly mixed uniformly；At 25 DEG C of temperature,
Under the conditions of pH6.0, Queensland nut special bio bacterial manure is made in screened and drying.
(3) Queensland nut special bio bacterial manure technical indicator
It is carried out according to Queensland nut special bio bacterial manure obtained in the present embodiment of agriculture ministerial standard NY/T798-2015 pair
Detection, as a result as follows:
(3.1) appearance: product appearance is graininess, without obvious mechanical admixture, uniform in size.
(3.2) technical indicator is shown in Table 3:
3 Queensland nut special bio bacterial manure technical indicator (embodiment 2) of table
| Project | Testing result |
| Living bacteria count (cfu), hundred million/g | It is qualified |
| Total nutrient (N+P2O5+K2O), % | It is qualified |
| Organic matter (to dry in terms of base), % | It is qualified |
| Miscellaneous bacteria rate, % | It is qualified |
| Moisture, % | It is qualified |
| PH value | It is qualified |
(3.3) innoxious index, is shown in Table 4:
The 4 innoxious index of Queensland nut special bio bacterial manure (embodiment 2) of table
| Project | Testing result |
| Excrement colibacillus group number, a/g | It is qualified |
| Induced worm egg death rate, % | It is qualified |
| Arsenic (As) (to dry in terms of base), mg/kg | It is qualified |
| Cadmium (Cd) (to dry in terms of base), mg/kg | It is qualified |
| Lead (Pb) (to dry in terms of base), mg/kg | It is qualified |
| Chromium (Cr) (to dry in terms of base), mg/kg | It is qualified |
| Mercury (Hg) (to dry in terms of base), mg/kg | It is qualified |
Embodiment 3: the fat example of Queensland nut special bacterium is prepared
(1) raw material preparation and preparation
(1.1) containing the fertilizer of a great number of elements
Nitrogenous fertilizer: selecting ammonium fertilizer and nitrate fertilizer to mix according to mass ratio 2:1, and ammonium fertilizer uses ammonium hydrogen carbonate to live
Property ingredient nitrogenous fertilizer (green river biology), nitrate fertilizer use sodium nitrate for the nitrogenous fertilizer of active constituent (green river is biological).
Phosphate fertilizer: use phosphorus pentoxide for the phosphate fertilizer (the Henan right way) of active constituent.
Potash fertilizer: use potassium sulfate for the potash fertilizer (western Shandong chemical fertilizer) of active constituent.
(1.2) trace element fertilizer in: middle trace element fertilizer is self-control, and each middle microelement is final concentration of: Chelating state zinc 2g/
L, Chelating state manganese 2g/L, boron 5g/L, sulphur 2g/L, magnesium 2g/L, calcium 2g/L, iron 1g/L, molybdenum 1g/L.
(1.3) humic acid (Chengdu lid Er Gaisi), is detected 4.1 weight % of water content, and the content of organic matter is 82 weight %.
(1.4) plant growth-promoting substance
By chitosan (Chengdu lid Er Gaisi) 1kg, amino acid fertilizer (deep logical fertile industry) 1kg, seaweeds (Qingdao Ostwald-Folin is grand)
0.1kg, root-inducing powder (Shandong Liangshan ancient cooking vessel is holy) 0.4kg mixing, are made plant growth-promoting mixture of substances.
(1.5) microbial bacterial agent:
Microbial bacterial agent includes bacillus licheniformis (A), bacillus megaterium (B), bacillus pumilus (C), waxy bud
Spore bacillus (D), colloid bacillus cereus (E)；Its production method is after 5 kinds of bacterial strains expand connect cultivation and fermentation respectively, and bacterium solution is with sterile
Solid absorbent absorption, after mixing well by weight A:B:C:D:E=3:1:1:2:3,30 DEG C of constant temperature stand dark culture 2 days,
Then it is dried under the conditions of 30 DEG C, moisture content is 30% to get microbial bacterial agent.
(1.6) bentonite (Sichuan and prosperous profit reach).
(1.7) organic fertilizer
Dish leaf 5kg, Queensland nut green peel 5kg, animal wastes 5kg, dregs of beans 1kg, vinasse 0.5kg, edible fungi residue 0.5kg
The EM bacteria agent (Shandong organic fertilizer factory) of final concentration of 3 weight % is added in mixing, and adjusting moisture content is 35 weight %, adjusts
PH value is 7.5, and decomposed 30 days to get the organic fertilizer.
(2) prepared by Queensland nut special bio bacterial manure
Take microbial bacterial agent 2kg, organic fertilizer 30kg, nitrogenous fertilizer 15kg, phosphate fertilizer 5kg, potash fertilizer 10kg, middle trace element fertilizer 3kg,
Humic acid 4kg, plant growth-promoting substance 1kg, bentonite 3kg, above-mentioned substance is thoroughly mixed uniformly；At 30 DEG C of temperature,
Under the conditions of pH6.5, Queensland nut special bio bacterial manure is made in screened and drying.
(3) Queensland nut special bio bacterial manure technical indicator
It is carried out according to Queensland nut special bio bacterial manure obtained in the present embodiment of agriculture ministerial standard NY/T798-2015 pair
Detection, as a result as follows:
(3.1) appearance: product appearance is graininess, without obvious mechanical admixture, uniform in size.
(3.2) technical indicator is shown in Table 5:
5 Queensland nut special bio bacterial manure technical indicator (embodiment 3) of table
| Project | Testing result |
| Living bacteria count (cfu), hundred million/g | It is qualified |
| Total nutrient (N+P2O5+K2O), % | It is qualified |
| Organic matter (to dry in terms of base), % | It is qualified |
| Miscellaneous bacteria rate, % | It is qualified |
| Moisture, % | It is qualified |
| PH value | It is qualified |
(3.3) innoxious index, is shown in Table 6:
The 6 innoxious index of Queensland nut special bio bacterial manure (embodiment 3) of table
| Project | Testing result |
| Excrement colibacillus group number, a/g | It is qualified |
| Induced worm egg death rate, % | It is qualified |
| Arsenic (As) (to dry in terms of base), mg/kg | It is qualified |
| Cadmium (Cd) (to dry in terms of base), mg/kg | It is qualified |
| Lead (Pb) (to dry in terms of base), mg/kg | It is qualified |
| Chromium (Cr) (to dry in terms of base), mg/kg | It is qualified |
| Mercury (Hg) (to dry in terms of base), mg/kg | It is qualified |
Comparative example 1: common farmyard manure
Farmyard manure is local common cow dung, it is decomposed dry after pulverize.
Comparative example 2: the bacterial manure of Queensland nut green peel is not added
This comparative example method for preparing fertilizer such as embodiment 1, when difference is to prepare organic fertilizer, does not add Australia heavily fortified point substantially
Fruit green peel.
Comparative example 3: the bacterial manure of chitosan is not added
This comparative example method for preparing fertilizer is substantially as embodiment 1 is not added when difference is to prepare plant growth-promoting substance
Chitosan.
Comparative example 4: the bacterial manure of microbial bacterial agent is not added
For this comparative example method for preparing fertilizer substantially such as embodiment 1, difference is that this comparative example does not add microbial bacterial agent
Test example 1: crop field randomized block experiment is carried out using the fertilizer prepared in embodiment 1-3 and comparative example 1-4
(1) Queensland nut seedling fertilization processing scheme
If 8 different fertilizations (table 7), group of not applying fertilizer is control group, and 3 repetitions of each processing are each to repeat to be arranged
One experimental plot, each plot area 12m*20m, random district's groups arrangement.Cell surrounding sets the two rows protection row of wide 5m.Using
The method of seedling balled transplanting, ridging transplant seedling.Field management is the same as local conventional cultivation.After transplanting, bacteriostatic agent is pressed into 1:500
Dilution proportion is uniformly poured and is applied until Queensland nut seedling root topsoil is wet (radius 5cm range around root), and every plant
The amount of pouring into of Queensland nut seedling is consistent, needs further according to practical growth, is watered with root water, remaining handles the water for pouring equivalent.Often
The dose of kind processing is 1.5kg/m2。
The design of 7 Queensland nut seedling crop field fertilizer test of table
(2) Testing index and data processing method
Queensland nut seedling plants field planting after, from July 1 to December 1, monthly 1 day in each cell Queensland nut children
The upgrowth situation of seedling is sampled investigation.Each cell takes 5 plants of Queensland nut seedling.Researching determining content is Queensland nut seedling
Aerial part (number of blade and fresh weight, dry weight) and under ground portion (root long, maximum root diameter and fresh weight, dry weight) character it is special
Sign.When measuring parameters for shape characteristic, weed growth situation in experimental plot is observed.Collected Queensland nut will monthly be investigated
Seedling root presses 7 numbering of table.Parameter interpretation is as follows:
The number of blade: the fresh Queensland nut seedling leaves number of length >=2cm.
Root long: the length of the main root of Queensland nut seedling.
Fresh weight: Queensland nut seedling root and leaf are separated, and measure each section fresh weight after removing extra soil.
Data processing is counted and is mapped using graphpad prism, carries out variance analysis and conspicuousness inspection to test data
It tests.
(3) test result
The situation of change of Queensland nut seedling leaves number (piece) is shown in that Fig. 1, abscissa are sample time under Different Fertilization, indulges and sits
It is designated as blade quantity (piece).
The variation of Queensland nut seedling leaf fresh weight (g) under Different Fertilization is shown in that Fig. 2, abscissa are sample time, and ordinate is
Leaf weight (gram).
The variation of Queensland nut seedling main root long (cm), is shown in that Fig. 3, abscissa are sample time, ordinate under Different Fertilization
For main root length (cm).
The variation of Queensland nut seedling root fresh weight (g) under Different Fertilization is shown in that Fig. 4, abscissa are sample time, and ordinate is
Root fresh weight (g).
As seen from the experiment, the growth of the dedicated bacterial manure of Queensland nut obtained in embodiment 1 to Queensland nut seedling
Development has preferable facilitation, and this facilitation is embodied in blade quantity and weight, main root length and root gross weight
On.Bacterial manure can provide Queensland nut seedling needed nutrient matter comprehensively in embodiment 1, effectively prevent disease, and have promotion to give birth to
Long effect.
The effect of bacterial manure is significantly better than common farmyard manure, the fertilizer, no for not adding Queensland nut green peel in embodiment 1/2/3
It adds the fertilizer of bacteria agent, add the fertilizer of chitosan.Experimental result shows, Queensland nut special fertilizer disclosed by the invention
Material can preferably promote Queensland nut growth of seedling, facilitation show promote seedling leaves quantity to increase, blade increases,
The growth of main root and lateral root.When measuring parameters for shape characteristic, weed growth situation in experimental plot, T2-T4, T6- are observed
In T7 hillslope processes, weed growth is less；And in T0, T1 and T5 hillslope processes, there is more weed growth, weeds are to Australia heavily fortified point
The effect of the competitive inhibition of growth of fruit treelet.
Experimental example 2: the research of Queensland nut peel extract allelopathy
(1) raw material, reagent and laboratory apparatus
Queensland nut pericarp picks up from Yunnan Province's Dehong prefecture, is air-dried, crushes (60 mesh), refrigerated spare.For trying allelopathic receptor
Weeds barnyard grass (Echinochloa crusgalli (L.) Beauv), field bindweed (Convolvulus arvensis L.), shepherd's purse
(Capsella bursa-pastoris (Linn.) and purslane (Portulaca oleracea L.) seed are by this laboratory
It provides.
Petroleum ether (analysis is pure) (Tianjin Fengchuan Chemical Reagent Science & Technology Co., Ltd.), (Tianjin causes chloroform (analysis is pure)
Remote chemical reagent Co., Ltd), ethyl acetate (analysis is pure) (six factory of Tianjin chemical reagent, three subsidiary factory), n-butanol (analysis is pure)
(Chongqing Kowloon chemical reagent factory), 95% ethyl alcohol (technical pure), hypochlorous acid receive (analysis pure) (Tianjin wind ship chemical reagent science and technology
Co., Ltd), sterile water, laboratory self-control.
Rotary Evaporators (RE-52) (Shanghai Yarong Biochemical Instrument Plant), (Zhengzhou circulating multiplex vavuum pump (SHB- III)
Great Wall science, industry and trade Co., Ltd), high-pressure sterilizing pot, (the permanent science and technology in Shanghai is limited for electric heating constant-temperature blowing drying box (DH-9140A type)
Company), ultrasonic cleaner, digital display thermostat water bath (HH-2) (Guo Hua Electrical Appliances Co., Ltd), refrigerator, condenser pipe, 1/10000
Assay balance, 1000ml separatory funnel, 1000ul liquid-transfering gun, pipette tips, 500ml beaker, 500ml conical flask, 100ml conical flask,
1000ml round-bottomed flask, 500ml round-bottomed flask, culture dish, 5ml, 50ml, 100ml graduated cylinder, qualitative filter paper, ruler, knitting wool.
(2) experimental method
(2.1) preparation of Queensland nut green peel extract
This experiment is to obtain accurately as a result, with the alcohol extract of Queensland nut green peel through methylene chloride extract return method to Australia
Nut green peel allelopathy ingredient extracts.
Queensland nut green peel dry sample 100g is weighed, the ethyl alcohol that addition 100mL volume fraction is 95% is crushed and is placed in
Filtering, suction filtration, collect filtrate after heating water bath flows back 3 hours in 200mL round-bottomed flask.Again plus volume fraction be 95% ethyl alcohol
Reflux, coprocessing 3 times.Then obtained green peel alcohol liquid is dried to paste, weighs after cooling, obtains paste peel extract
10.91g.Gained green peel medicinal extract is dissolved into aqueous solution with 500ml distilled water, it is spare.
Solution after resulting petroleum ether extraction is poured into separatory funnel, isometric methylene chloride is added, shakes up, it is quiet
Extraction 5 hours is set, lower layer's solution is taken.Isometric methylene chloride is added into upper solution again to be extracted, in triplicate.It will
The methylene chloride extract layer solution mixing obtained three times, is spin-dried for, dichloromethane extract of weighing to obtain with Rotary Evaporators: 1.5g,
Then plus distilled water is diluted to the dichloromethane extract stoste of 0.1g/mL, and sealing is placed in 4 DEG C of refrigerators and saves backup.
(2.2) the gradient processing of extracting solution
By Queensland nut green peel extracting solution stoste be diluted to respectively 10mg/mL, 5mg/mL, 2.5mg/mL, 1mg/mL,
Seven different quality concentration gradients of 0.5mg/mL, 0.25mg/mL, 0.125mg/mL are control (CK) with distilled water.
(2.3) for planting experimentally sub processing
By barnyard grass (Echinochloa crusgalli (L.) Beauv), field bindweed (Convolvulus arvensis
L.), shepherd's purse (Capsella bursa-pastoris L) and purslane (Portulaca oleracea L.) seed all rinse
Completely, 20min is impregnated with the sodium hypochlorite that volume fraction is 5% respectively, then with aseptic water washing 4-5 times, finally uses distilled water
2h is impregnated, Seed imbibition is made to be conducive to germination.
(2.4) seed sprouts experiment
It supplies to plant experimentally from 4 kinds handled well and selects full grains in son, tested without the intact seed of breach, respectively
It is put into the culture dish (diameter 15cm) for being covered with filter paper, every ware puts 20.Pour into the treatment fluid of each concentration of 10mL respectively, every kind
Processing all sets 3 repetitions.Plate is placed on constant temperature incubation bed and is cultivated, temperature is set as 25 DEG C, purchases bed by oneself and plays timing, to when germination
Routine observation.Per the growing state and supplement process liquid for observing a seed for 24 hours, remain that filter paper soaks.Germination the 3rd
It when count germination number, calculate germinating energy；Germinative number is counted at the 5th day again, calculates germination percentage.
Subnumber × 100% is planted experimentally in seed number/confession that germinating energy=preceding 3d normally germinates
Subnumber × 100% is planted experimentally in seed number/confession that germination percentage=preceding 5d normally germinates
(2.5) data analysing method
Germination percentage, the germinating energy of survey calculation seed are analyzed with Excel and are mapped.
(3) experimental result
As shown in Figure 5, the Queensland nut green peel dichloromethane extract of different quality concentration is carried out to several for studying object
After processing, germinating energy and germination percentage are declined than control group, illustrate Queensland nut green peel dichloromethane extract to kind
Son, which is sprouted, certain inhibiting effect, and the mass concentration for the treatment of fluid is higher, and inhibiting effect is stronger.Australia of different quality concentration
After the processing of nut green peel dichloromethane extract, the germination percentage and control group difference of barnyard grass and field bindweed seed be not significant, but its
All there were significant differences for the germinating energy and germination percentage and control group of his crop seed, and the dichloromethane extract of more high concentration
It is more significant with control group difference after processing.When dichloromethane extract mass concentration is 10mg/ml, 5mg/ml, 2.5mg/ml, shepherd's purse
The germinating energy of colza and germination percentage are 0, illustrate that it has very strong inhibiting effect to shepherd's purse, can be to its death, we can be with
It is affected to dichloromethane extract to crucifer according to this supposition.Overall, Queensland nut dichloro
The influence that methane takes extraction object to sprout four seeds: shepherd's purse inhibiting effect is most strong, and purslane takes second place, and field bindweed and barnyard grass are most weak.
Experimental example 3: influence of the antimicrobial composition to root system endogenous growth hormone content
(1) experimental design and material processing
This research uses earth pillar method: the engineering plastics hard tube of long 1.5m, diameter 0.2m taken, is stood in the heatable adobe sleeping platform of deep 1.5m,
It is packed into simulation soil in pipe, organic matter 7.7g/kg, nitrogen 50mg/kg, P elements 3.8mg/kg, potassium member are added in the soil
Plain 12.3mg/kg, pH5.2.Each die-filling quasi- soil 25kg of root canal.
Current year September transplants annual vegetative propagation seedling, 1 plant of each root canal；Queensland nut Miao Genyong clear water is floated before planting
Wash clean simultaneously sterilizes (0.5% sodium hypochlorite), then transplants after being rinsed with distilled water, each 10 plants of processing.Next year September takes out test
Indices.When plant, heatable adobe sleeping platform is dug out in soil in pipe, microbial bacterial agent 1kg is dispersed evenly in heatable adobe sleeping platform, is then placed in
Seedling backfills soil, pours suitable quantity of water.Wherein, microbial bacterial agent A: clothing bacillus, bacillus megaterium, bacillus pumilus,
The mass ratio of bacillus cereus and colloid bacillus cereus is 3:2:1:3:3；Microbial bacterial agent B: clothing bacillus, huge gemma
Bacillus, bacillus pumilus, bacillus cereus and colloid bacillus cereus mass ratio be 3:2:2:3:5；Microbial bacterial agent C:
Clothing bacillus, bacillus megaterium, bacillus pumilus, bacillus cereus and colloid bacillus cereus mass ratio be 2:1:
1:2:3.Blank control is directly by Seeding planting in earth pillar.Conventional fertilizer application is carried out to Queensland nut seedling during culture.Experiment
Design is as shown in following table (table 8):
Table 8: the experimental design of experimental example 3 and experimental example 4
After culture, Queensland nut seedlings root is taken, by material liquid nitrogen flash freezer, is subsequently placed into -80 DEG C of ultralow temperature ices
It is saved in case, to subsequent hormone determination.
(2) main agents and instrument
Auxin (IAA) standard specimen (purity >=99.5% is purchased from Sigma company), methanol (chromatographically pure, western Gansu Province science share
Co., Ltd), formic acid (analyzes pure, western Gansu Province science limited liability company), and ammonium hydroxide (analyzes pure, the western limited public affairs of Gansu Province science share
Department), test water is ultrapure water, and mixed anion exchanges reverse phase solid phase extraction column Cleanert SAX and (is purchased from Bonna-Agela
Technologies company).Superelevation liquid chromatogram-triple level four bars mass spectrometer (Waters ACQUITY UPLC H-
Class&waters Xevo TQD)。
(3) endogenous growth hormone extracts
The specimen material gathered in advance is taken out from -80 DEG C of ultra low temperature freezers, precise 0.5g adds in mortar
Enter liquid nitrogen grinding to crushing；Sample powder is poured into centrifuge tube, and 80% methanol of pre-cooling, dark condition are added into centrifuge tube
Lower 4 DEG C of extractions are for 24 hours；10,000rpm is centrifuged 10min at 4 DEG C after extraction；It sucts clearly in new centrifuge tube, residue is used again
4mL extracting solution extracts 2h, and 4 DEG C of 10,000rpm are centrifuged 10min；Merge supernatant twice.Reverse phase solid phase is exchanged with mixed anion
Endogenous growth hormone in extraction column Cleanert SAX extraction purification supernatant.
(4) efficient liquid phase mass-spectrometer measurement endogenous growth cellulose content
High performance liquid chromatography testing conditions are as follows: column temperature is set as room temperature, and mobile phase uses -1% acetic acid (volume ratio of methanol
Be 40: 60) solution (being filtered using preceding with 0.45 μm of filter membrane), flow rate set 1.0ml/min, ultraviolet detection wavelength is set as
269nm, sample volume are 20 μ l.
(5) experimental result
It is as shown in Figure 6 that Queensland nut fruit tree root system Endogenous auxin (IAA) analyzes result.It will be appreciated from fig. 6 that using embodiment
The microbial bacterial agent of 1-3 preparation can reduce Endogenous auxin level in root system, and microbial bacterial agent is to Endogenous auxin in root system
Level has fine tuning effect, can make the horizontal pico- reduction of the auxin accumulated in main root, to eliminate higher concentration growth
The inhibiting effect that element grows main root, so that root system be promoted to grow to deep subsoil.
Experimental example 4: influence of the antimicrobial composition to root growth and development
(1) experimental design
This research uses earth pillar method: the engineering plastics hard tube of long 1.5m, diameter 0.2m taken, is stood in the heatable adobe sleeping platform of deep 1.5m,
It is packed into simulation soil in pipe, organic matter 7.7g/kg, nitrogen 50mg/kg, P elements 3.8mg/kg, potassium member are added in the soil
Plain 12.3mg/kg, pH5.2.Each die-filling quasi- soil 25kg of root canal.
Current year September transplants annual vegetative propagation seedling, 1 plant of each root canal；Queensland nut Miao Genyong clear water is floated before planting
Wash clean simultaneously sterilizes (0.5% sodium hypochlorite), then transplants after being rinsed with distilled water, each 10 plants of processing.Next year September takes out test
Indices.When plant, heatable adobe sleeping platform is dug out in soil in pipe, microbial bacterial agent 1kg is dispersed evenly in heatable adobe sleeping platform, is then placed in
Seedling backfills soil, pours suitable quantity of water.Wherein, microbial bacterial agent A: clothing bacillus, bacillus megaterium, bacillus pumilus,
The mass ratio of bacillus cereus and colloid bacillus cereus is 3:2:1:3:3；Microbial bacterial agent B: clothing bacillus, huge gemma
Bacillus, bacillus pumilus, bacillus cereus and colloid bacillus cereus mass ratio be 3:2:2:3:5；Microbial bacterial agent C:
Clothing bacillus, bacillus megaterium, bacillus pumilus, bacillus cereus and colloid bacillus cereus mass ratio be 2:1:
1:2:3.Positive controls are impregnated Queensland nut seedlings root 48 hours with 150mg/L heteroauxin solution, take seedling fixed later
It plants in earth pillar.Blank control is directly by Seeding planting in earth pillar.Conventional fertilizer application is carried out to Queensland nut seedling during culture.
Experimental design is as shown in table 8.
(2) root system and physiological parameter measurement
Root system sample after careful harvest is stored in immediately in 25% alcoholic solution, then passes through digital scanner
Complete root system image scanning is carefully stored in computer by (STD1600Epson USA), later with root system matched with scanner
Analysis system software carries out quantitative analysis to main root length, lateral root total length, lateral root number.After the completion of Root Parameters analysis, survey
Determine root physiological parameter.After root system is washed with water completely, in 110 DEG C of water-removing 15min, drying to constant weight at 75 DEG C, measurement
Root system gross weight, surveys dry weight respectively after taproot and lateral root is separated.
(3) result and its analysis
See Table 9 for details for experimental result, and microbial bacterial agent has facilitation to the growth of Queensland nut main root according to the experimental results,
And it is remarkably improved the ratio of main root dry weight.The growth of main root allows Queensland nut fruit tree more steady bundle in the soil
Root improves wind loading rating.Lateral root is grown from main root, since the lengthening of main root length provides more spaces for the hair of lateral root
Raw, lateral root number increases.The tip of a root position of root is the main portions for absorbing nutrient and moisture, and lateral root number increases, it is meant that is produced
More tips of a root are given birth to absorb nutrient and moisture, to promote plant growth.Number is the experimental group and number of 2T1,2T2,2T3
It is compared for the positive controls of 2T0, the difference of lateral root total length is smaller, but uses the microbial bacterial agent group prepared in embodiment 1-3
Closing object processing Queensland nut sapling can be such that lateral root number increases under the premise of lateral root total length is constant, so that effectively
Nutrient and water absorption site increases, to promote the development growth of Queensland nut trees.In addition, due to the growth of main root, side
Root can be distributed in bigger vertical space, can use the nutriment and moisture of deep subsoil, avoided lateral root and only existed
A possibility that soil shallow-layer is excessively grown, and reduces the lateral root mutually overlapping phenomenon for different fruit trees occur, reduce fruit tree it
Between intraspecific competition.
Table 9: root system and physiological parameter measurement result
| Number | Main root is long (cm) | Main root dry weight accounting (%) | Lateral root total length (cm) | Lateral root number (root) | Lateral root specific gravity (%) |
| 2T1 | 51.48±2.89 | 34.1 | 8917±297 | 6015±312 | 75.2 |
| 2T2 | 49.63±4.28 | 30.7 | 8749±314 | 5789±179 | 78.4 |
| 2T3 | 50.21±5.22 | 29.1 | 8694±155 | 5487±215 | 79.2 |
| 2T0 | 42.36±10.70 | 13.6 | 8713±284 | 4584±158 | 85.3 |
| 2CK | 37.51±2.85 | 17.1 | 6537±163 | 4089±263 | 82.7 |
What has been described above is only an embodiment of the present invention, and the common sense such as well known specific structure and characteristic are not made herein in scheme
Excessive description.It, without departing from the structure of the invention, can be with it should be pointed out that for those skilled in the art
Several modifications and improvements are made, these also should be considered as protection scope of the present invention, these all will not influence what the present invention was implemented
Effect and patent practicability.The scope of protection required by this application should be based on the content of the claims, in specification
The records such as specific embodiment can be used for explaining the content of claim.
Claims (10)
1. a kind of Queensland nut special bio bacterial manure, which is characterized in that be made from the following raw materials in parts by weight: 2-5 parts of micro- life
Object microbial inoculum, the green peel containing Queensland nut 10-20 parts of 5-8 parts of 15-22 parts of 30-45 parts of organic fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, in it is micro
3-7 parts of element fertilizer, 4-8 parts of humic acid, 1-3 parts of plant growth-promoting substance and 3-7 parts of bentonite.
2. a kind of Queensland nut special bio bacterial manure according to claim 1, which is characterized in that described green containing Queensland nut
The organic fertilizer of skin is made from the following raw materials in parts by weight: 5 parts of dish leaf, 5 parts of Queensland nut green peel, 5 parts of animal wastes, 1 part of dregs of beans,
0.5 part and 0.5 part of edible fungi residue of vinasse.
3. a kind of Queensland nut special bio bacterial manure according to claim 2, which is characterized in that described green containing Queensland nut
The organic fertilizer of skin comprises the following steps: dish leaf, Queensland nut green peel, animal wastes, dregs of beans, vinasse, edible fungi residue are pressed
Ratio mixing, is added EM bacteria agent, adjusts the 30-35% that moisture content is weight, and adjusting pH value is 6.5-7.5, decomposed 30-45
It to get.
4. a kind of Queensland nut special bio bacterial manure according to any one of claim 1-3, which is characterized in that in described
Trace element fertilizer is made from the following raw materials in parts by weight: 1-2 parts of Chelating state zinc, 1-2 parts of Chelating state manganese, 3-5 parts of boron, 1-2 parts of sulphur,
1-2 parts of magnesium, 2-5 parts of calcium, 1-2 parts of iron, 0.5-1 parts of molybdenum.
5. a kind of Queensland nut special bio bacterial manure according to any one of claim 1-3, which is characterized in that the nitrogen
Fertilizer is any one of ammonium fertilizer, nitrate fertilizer, amide nitrogen fertilizer and organic nitrogenous fertilizer or any two or two or more groups
It closes；The phosphate fertilizer is phosphorus pentoxide；The potash fertilizer be any one of potassium oxide, potassium sulfate and potassium chloride any two or two
Kind or more combination.
6. a kind of Queensland nut special bio bacterial manure according to any one of claim 1-3, which is characterized in that the corruption
The water content of phytic acid is 3-5 weight %, and the content of organic matter of the humic acid is greater than or equal to 75 weight %.
7. a kind of Queensland nut special bio bacterial manure according to any one of claim 1-3, which is characterized in that the plant
Object growth-promoting substance is made from the following raw materials in parts by weight: 1-2 parts of chitosan, 0.1-0.2 parts of seaweeds, is taken root at -2 parts of amino acid 1
0.2-0.5 parts of powder.
8. a kind of Queensland nut special bio bacterial manure according to any one of claim 1-3, which is characterized in that described micro-
Bacteria agent includes the raw material bacterial strain of following parts by weight: 2-3 parts of bacillus licheniformis, 1-2 parts of bacillus megaterium, short and small gemma
1-2 parts of bacillus, 2-3 parts of bacillus cereus and 3-5 parts of colloid bacillus cereus.
9. a kind of Queensland nut special bio bacterial manure according to claim 8, which is characterized in that the microbial bacterial agent by
Following method is made:
Step a: by bacillus licheniformis, bacillus megaterium, bacillus pumilus, bacillus cereus and colloid bacillus cereus
Expand respectively and connect big cultivation and fermentation, obtains 5 kinds of zymocyte liquids；
Step b: separation obtains 5 kinds of thallus respectively from 5 kinds of zymocyte liquids in step a, and 5 kinds of thallus are sufficiently mixed in proportion
It is even, obtain Mixed Microbes；
Step c: the Mixed Microbes in step b are expanded culture, mixed bacteria liquid is obtained；
Step d: separation obtains mixing thallus from the mixed bacteria liquid in step c, by mixing thallus drying to get the microorganism
Antimicrobial composition.
10. a kind of preparation method of Queensland nut special bio bacterial manure according to claim 1,2 or 3, including following step
It is rapid: to match by regulation by microbial bacterial agent, organic fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, middle trace element fertilizer, humic acid, plant growth-promoting
Substance, bentonite are thoroughly mixed uniformly；At 25-30 DEG C of temperature and pH 6.0-6.8, screened and drying is made.
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