CN103086771B - Bioorganic fertilizer preparation method, bioorganic fertilizer and application of bioorganic fertilizer - Google Patents
Bioorganic fertilizer preparation method, bioorganic fertilizer and application of bioorganic fertilizer Download PDFInfo
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- CN103086771B CN103086771B CN201310045294.5A CN201310045294A CN103086771B CN 103086771 B CN103086771 B CN 103086771B CN 201310045294 A CN201310045294 A CN 201310045294A CN 103086771 B CN103086771 B CN 103086771B
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Abstract
The invention relates to the technical field of biological fertilizers, and discloses a bioorganic fertilizer preparation method, a bioorganic fertilizer and an application of the bioorganic fertilizer. The bioorganic fertilizer is prepared by mixing high-fiber organic matters comprising rice bran, soybean meal, corn flour, peanut bran and the like and beneficial microbes according to a reasonable ratio through a technology. The prepared bioorganic fertilizer has a pitch-black and bright appearance, has a soy fragrance, and has effective living bacteria count of 0.05 billion cuf/g or more, an organic matter content of 47% or more, a water content of 25% or less, a Fecal coliform count of 0.11/g or less, and a roundworm mortality of 95%, and all the indexes are better than Chinese standards. The preparation method has the advantages of no use of animal waste, clean raw material source, and clean and odorless production workshop, creates a good work environment for production works, and accords with the environmental protection demand.
Description
Technical field
The present invention relates to bio-feritlizer technical field, more specifically, relate to a kind of preparation method of biological organic fertilizer and the biological organic fertilizer preparing and application.
Background technology
Biological organic fertilizer refers to microorganisms with specific functions and mainly has the fertilizer of microbial fertilizer and fertilizer effect take plant and animal residues (as feces of livestock and poultry, agricultural crop straw etc.) concurrently for a class of originating and be composited through harmless treatment, the organic materials that becomes thoroughly decomposed.Biological organic fertilizer nutritive element is complete, can improve soil, improves the soil compaction that uses chemical fertilizer to cause.Improve soil physical and chemical property, strengthen the ability of soil water-retaining, fertilizer conservation, fertilizer.Beneficial microorganism in biological organic fertilizer forms mutual symbiosis Relationship With Proliferation with Soil Microorganism after entering soil, suppressing harmful bacteria grows and is converted into probiotics, interact, mutually promote, play the synergy of colony, probiotics produces a large amount of meta-bolitess in growth and breeding process, impels organic decomposition and inversion, can be directly or indirectly for crop provides multiple nutrients and pungent, promote and regulation and control plant growth.Improve soil porosity, penetrating exchangeability and plant survival rate, increase probiotics and soil microorganisms and population.Meanwhile, the advantage probiotics group energy forming at crop root suppresses harmful pathogenic bacteria procreation, strengthens the degeneration-resistant resistance against diseases of crop and reduces the disease index of continuous cropping crop, uses year after year and can greatly alleviate continuous cropping obstacle.Reducing environmental pollution, to people, animal, environmental safety, nontoxic, is a kind of environment-friendly type fertilizer.
But at present, biological organic fertilizer of the prior art, in order to reach certain fertilizer efficiency, has all used animal excrement, has used the poultry manures such as chicken manure, sheep excrement, pig manure as the bio-feritlizer in patent CN102633539, this causes production plant to produce unpleasant stink, and production environment is severe.
Biological fermentation is subject to many factors, and the raw material of different ingredients and proportioning the obtained obvious technical effects of fermenting is different, and the physico-chemical property of fertilizer and fertilizer efficiency are also significantly different.Only in reasonably formula and proportioning and under in conjunction with simple and effective preparation method, could finally obtain environmental protection, green, biological organic fertilizer that fertilizer efficiency is good.
Summary of the invention
The object of the invention is to overcome the technical deficiency of existing biological organic fertilizer, a kind of preparation method of biological organic fertilizer is provided, adopts the inventive method, successfully prepare clean, fertilizer efficiency good and have the bio-organic fertilizer of soy sauce fragrance, simple to operate, environment is good.
Another object of the present invention is to provide the fertilizer that described preparation method prepares.
A further object of the invention is to provide the application of described biological organic fertilizer.
Object of the present invention is achieved by the following technical programs:
The preparation method that a kind of biological organic fertilizer is provided, comprises the following steps:
S1. rice bran, dregs of beans, peanut press pulp and bean dregs are mixed in proportion to obtain to mixture, by mixture tiling, with the mixture after inosine mother liquor nutritive medium immersion tiling, add yeast, in 50～60 ℃ of bottom fermentations, in fermenting process, carry out turning processing;
After having fermented described in S2.S1, the temperature of mixture is down to 26～30 ℃, adds microbial nutrient solution, the microorganism in microbial nutrient solution breeds 10～20 days in mixture, obtains described biological organic fertilizer.
The applicant, based on years of researches analysis and summary, is devoted to high fiber organism to prepare high-quality biological fertilizer, with stench, pollution and the secondary pollution avoiding using ight soil and municipal sludge to be caused always.But long-term a large amount of experimental summary is found, if the organic selection compatibility of high fiber is unreasonable, can not be for multiple beneficial microorganism provide mutual utilization and the mutual good environment of symbiosis, be also just difficult to real realize simultaneously obtain clean, fertilizer efficiency is good and have the biological organic fertilizer of soy sauce fragrance.
The present invention adopts rice bran, dregs of beans, peanut press pulp and bean dregs to mix in proportion and obtains basic mixture, dregs of beans is a kind of byproduct that soybean obtains after extracting bean oil, according to the method difference of extracting, can be divided into and soak dregs of beans and two and soak two kinds of dregs of beans, wherein soak dregs of beans take the byproduct after extraction extracting bean oil as one, and first get oil with squeezing, then the byproduct of gained is called two and soaks dregs of beans after oil is got in lixiviate.Make the bean dregs of soya-bean milk generation and almost forgotten by people because mouthfeel is poor, usually used as waste.But a large amount of protein is along with the process of extracting soya-bean milk is extracted away in bean dregs, in bean dregs, contain protein and a large amount of Mierocrystalline cellulose of small part, dregs of beans is the product after extracting bean oil, has higher protein content, higher than bean dregs.The present invention by the two according to dregs of beans 5～15 weight parts, the ratio of bean dregs 5～15 weight parts matches, be equipped with again rice bran 60～80 weight parts, peanut press pulp 5～15 weight parts, the indexes such as the carbon-nitrogen ratio in fermented material are improved, experimental results show that described compatibility and proportioning have played the effect of the fermentation that heats up better, provide good biological organic fertilizer fermenting mixture material matrix.
More preferably, the parts by weight of rice bran, dregs of beans, peanut press pulp and bean dregs are: 70 parts, rice bran, 10 parts of dregs of beans, 10 parts of peanut press pulps, 10 parts, bean dregs.
S1 soaks the mixture of tiling with inosine mother liquor nutritive medium as long as guarantee that the mixed material of inosine mother liquor nutritive medium liquid surrounds.Preferably, described in S1 the consumption of inosine mother liquor nutritive medium according to the gross weight of mixture: the volume of inosine mother liquor nutritive medium is that 10Kg:1L determines.
Preferably, in fermenting process, after 5th～7 days, add the inedible oil fat that accounts for mixture quality 1.5～2% described in S1; More preferably, can add the inedible oil fat of mixture quality 1.5%.Described inedible oil fat preferably glycerine or edible waste oil (waste grease for example producing in diet processes).
Preferably, turning processing is after 5～7 days, mixture to be carried out to turning for the first time in fermentation described in S1, once every turning in 1～3 month later; The time of described fermentation is 5～6 months.Described overthrowing according to material quantity, can be used iron Chinese catalpa or navvy to overthrow.
More preferably, described in S1, when carrying out turning for the first time to mixture after 5～7 days, fermentation adds the inedible oil fat that accounts for mixture (material that rice bran, dregs of beans, peanut press pulp, bean dregs and microbial nutrient solution mix) quality 1.5～2%.Preferably, can add the inedible oil fat of mixture quality 1.5%; Described inedible oil fat preferably glycerine or edible waste oil.
Preferably, described in S2, in microbial nutrient solution, contain phosphate solubilizing bacteria, potassium solubilizing bacteria and vinelandii.Described in S2, in every milliliter of microbial nutrient solution, contain: phosphate solubilizing bacteria 0.1～0.3 hundred million cuf/mL, potassium solubilizing bacteria 0.1～0.3 hundred million cuf/mL and vinelandii 0.1～0.3 hundred million cuf/mL.The present invention uses described three kinds of probioticss, and rational compatibility and addition are accurately determined, under step of preparation process provided by the invention and condition, the System forming forming with described mixture utilizes mutually, the coenocorrelation of mutual symbiosis, finally guarantee that the biological organic fertilizer fertilizer efficiency preparing is good, production process odorless.
Most preferably, described in S2, in every milliliter of microbial nutrient solution, contain: phosphate solubilizing bacteria 0.2 hundred million cuf/mL, potassium solubilizing bacteria 0.2 hundred million cuf/mL and vinelandii 0.2 hundred million cuf/mL.
Preferably, the preparation method of the microbial nutrient solution described in S2 is: phosphate solubilizing bacteria, potassium solubilizing bacteria and vinelandii are rendered in inosine mother liquor nutritive medium according to addition, and cellar culture obtains, and is preferable over 20～30 ℃ and cultivates 50～60 days and get final product.
In described microbial nutrient solution, described phosphate solubilizing bacteria be preferably bacillus megaterium (
bacillus megaterium), described potassium solubilizing bacteria be preferably silicate bacteria (
silicate bacteria), described vinelandii be preferably vinelandii (
azotobacter sp), be all conventional purchase.
Preferably, described in S2 the consumption of microbial nutrient solution according to the gross weight of mixture: the volume of microbial nutrient solution is that 10Kg:2L determines.
The invention provides the biological organic fertilizer that described preparation method prepares, simple, production environment cleans odorless.Prepare biological organic fertilizer according to step of the present invention and technique, the technique effect that different batches obtains is highly stable.The equal outward appearance of biological organic fertilizer preparing is pitch-black glossy, has soy sauce fragrance, and living bacteria count is not less than 0.5 hundred million cuf/g, organic matter is not less than 47%, and moisture is not more than 25%, and colibacillus of excrement number is not more than 0.11/g, roundworm mortality ratio is not less than 95%, and all indexs are all better than national standard.
The present invention also provides the application of described biological organic fertilizer, is applicable to agroforestry crop production, is specially adapted to the application aspect plantation lichee, cabbage heart, shatian pomelo or paddy rice.
The present invention has following beneficial effect:
The present invention is with rice bran, dregs of beans, Semen Maydis powder, peanut press pulp prepares biological organic fertilizer jointly as high fiber organism and beneficial microorganism, by microorganism fermentation and organic physico-chemical property are creatively analyzed also in conjunction with long-term a large amount of experiment, find out suitable proportioning raw materials, success obtains biological organic fertilizer of the present invention, biological organic fertilizer has photosynthetic property, antiviral property, decompose soil nutrient, the multiple ferment of growth-promoting, the good microorganism biological characteristics such as fiber decomposability, fertilizer efficiency is good, distribute soy sauce fragrance, thoroughly change the dirty of conventional fertilizers, the defect such as smelly, for applying of biological organic fertilizer provides strong technical support.
The bio-organic fertilizer that the present invention prepares does not use animal excrement, and raw material sources are clean, under suitable production method step operation, has guaranteed that production plant is clean, and odorless, for direct labor has created good Working environment, meets environmental protection demand.
Further, the present invention is the control to critical process in preparation process, and the processing step to preparation and proportioning raw materials and addition sequence have done creationary improving and optimizating, both kill harmful microorganisms, guarantee the abundant fermentative action of beneficial microorganism, starting material are carried out to slaking decomposition; In biological organic fertilizer of the present invention, use the inedible oil fat of sufficient quantity, fertilizer can become moistening thickness, there is preserve moisture and fertility effect, the fertilizer efficiency that has guaranteed organic fertilizer of the present invention rises to 24 months, compared with the general bio-organic fertilizer fertilizer efficiency of 6 months, phenomenal growth, stability significantly improves.
After testing, biological organic fertilizer outward appearance of the present invention is pitch-black glossy, has soy sauce fragrance, living bacteria count is not less than 0.5 hundred million cuf/g, and organic matter is not less than 47%, and moisture is not more than 25%, colibacillus of excrement number is not more than 0.11/g, and roundworm mortality ratio is not less than 95%, and all indexs are all better than national standard.
Embodiment
Further explain the present invention below in conjunction with specific embodiment, unless stated otherwise, the raw material that the embodiment of the present invention adopts and method are the conventional raw material of the art and method.For convenience of description, the inosine mother liquor nutritive medium described in the embodiment of the present invention is directly to adopt the inosine liquid purchased from Xinghu Biotech Co., Ltd., Zhaoqing City, Guangdong Prov..Yeast described in the embodiment of the present invention is purchased from microbiological analysis inspection center of Guangdong Province, and Article Number is AS2.180.Those skilled in the art of the present technique also can adopt the similar product in other buying sources.Unless stated otherwise, the raw material adopting in the embodiment of the present invention and equipment are raw material and the equipment in the conventional source of the art.
embodiment 1
By bacillus megaterium (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is ACCC11011), silicate bacteria is (purchased from Agricultural University Of South China, Article Number is IM6), vinelandii are (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is AS1.222) render in inosine mother liquor nutritive medium and cultivate, make to contain in every milliliter of microbial nutrient solution: phosphate solubilizing bacteria 0.1 hundred million cuf/mL, potassium solubilizing bacteria 0.1 hundred million cuf/mL, vinelandii 0.1 hundred million cuf/mL.Cultivation obtains the microbial nutrient solution that contains various trace elements for 50～60 days at 25～30 ℃, (not need camera bellows).Above-mentioned bacterial strains is not limited to described purchasing channel, can be also self-control or other sources.Inosine mother liquor nutritive medium mainly contains organic matter, nitrogen, phosphorus, potassium, humic acid and glucose.
Prepare biological organic fertilizer step as follows:
S1. by 60 parts, rice bran, 5 parts of dregs of beans, after 5 parts of raw material blendings such as grade of 5 parts of peanut press pulps and bean dregs, obtain mixture, mixture is tiled, preferably the thickness of tiling is 10cm, press the mixture (guaranteeing that the mixed material of liquid surrounds) that 1Kg:0.1L inosine mother liquor nutritive medium soaks tiling, in mixture, add yeast (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is AS2.180, add-on is 6L, 8000000000 cuf/L) be maintained at 55～60 ℃ of fermentations, ferment, after 5～7 days, starting material are carried out to turning, later every turning in 1～3 month 1 time, continuing fermentation 5～6 months, mixture slaking is decomposed,
The temperature of the mixture after having fermented described in S2.S1, slaking having been decomposed is down to 26～30 ℃, gross weight according to mixture: the volume of microbial nutrient solution is that 10Kg:2L adds microbial nutrient solution, in the mixture that microorganism in microbial nutrient solution is decomposed in slaking, breed 10～20 days, obtain biological organic fertilizer of the present invention.Fertilizer is air-dry is powdery after dry, and outward appearance is pitch-black glossy, has soy sauce fragrance, machinery-free impurity.
embodiment 2
By bacillus megaterium (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is ACCC11011), silicate bacteria is (purchased from Agricultural University Of South China, Article Number is IM6), vinelandii are (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is AS1.222) render in inosine mother liquor nutritive medium and cultivate, make to contain in every milliliter of microbial nutrient solution: phosphate solubilizing bacteria 0.2 hundred million cuf/mL, potassium solubilizing bacteria 0.2 hundred million cuf/mL, vinelandii 0.2 hundred million cuf/mL.Cultivation obtains the microbial nutrient solution that contains various trace elements for 50～60 days at 25～30 ℃, (not need camera bellows).Above-mentioned bacterial strains is not limited to described purchasing channel, can be also self-control or other sources.Inosine mother liquor nutritive medium mainly contains organic matter, nitrogen, phosphorus, potassium, humic acid and glucose.
S1. by after the raw material blendings such as 15 parts, 80 parts, rice bran, 15 parts of dregs of beans, 15 parts of peanut press pulps and bean dregs mixture, by mixture tiling, preferably the thickness of tiling is 12cm; Soak the mixture of tiling by 1Kg:0.1L inosine mother liquor nutritive medium (being purchased from Xinghu Biotech Co., Ltd., Zhaoqing City, Guangdong Prov.), add yeast (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is AS2.180, add-on is 6L, the same), be maintained at 55～60 ℃ of fermentations, ferment, after 5～7 days, starting material are carried out to turning, once every turning in 1～3 month later; Continuing fermentation 5～6 months, mixture slaking is decomposed;
The temperature of the mixture after S2.S1 has fermented, slaking having been decomposed is down to 26～30 ℃, add the gross weight according to mixture: the volume of microbial nutrient solution is that 10Kg:2L adds microbial nutrient solution (preparation method is with embodiment 1), in the mixture that microorganism in microbial nutrient solution is decomposed in slaking, breed 10～20 days, obtain biological organic fertilizer of the present invention, after dry, be powdery, outward appearance is pitch-black glossy, has soy sauce fragrance, machinery-free impurity.
embodiment 3
By bacillus megaterium (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is ACCC11011), silicate bacteria is (purchased from Agricultural University Of South China, Article Number is IM6), vinelandii are (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is AS1.222) render in inosine mother liquor nutritive medium and cultivate, make to contain in every milliliter of microbial nutrient solution: phosphate solubilizing bacteria 0.3 hundred million cuf/mL, potassium solubilizing bacteria 0.3 hundred million cuf/mL, vinelandii 0.3 hundred million cuf/mL.Cultivation obtains the microbial nutrient solution that contains various trace elements for 50～60 days at 25～30 ℃, (not need camera bellows).Above-mentioned bacterial strains is not limited to described purchasing channel, can be also self-control or other sources.Inosine mother liquor nutritive medium mainly contains organic matter, nitrogen, phosphorus, potassium, humic acid and glucose.
S1. by after the raw material blendings such as 10 parts, 70 parts, rice bran, 10 parts of dregs of beans, 10 parts of peanut press pulps and bean dregs mixture, by mixture tiling, preferably the thickness of tiling is 15cm; Press the mixture that 1Kg:0.1L inosine mother liquor nutritive medium soaks tiling, add yeast (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is AS2.180, add-on is 7L), be maintained at 60～68 ℃ of fermentations, ferment, after 5～7 days, mixture is carried out to turning, in turning, add the glycerine that accounts for mixture quality 1.5%, the moistening thickness of material; Once every turning in 1～3 month later, continuing fermentation 5～6 months, mixture slaking is decomposed;
The temperature of the mixture after S2.S1 has fermented, slaking having been decomposed is down to 26～30 ℃, add the gross weight according to mixture: the volume of microbial nutrient solution is that 10Kg:2L adds microbial nutrient solution (preparation method is with embodiment 1), in the mixture that microorganism in microbial nutrient solution is decomposed in slaking, breed 10～20 days, obtain biological organic fertilizer of the present invention, after dry, be powdery, outward appearance is pitch-black glossy, has soy sauce fragrance, machinery-free impurity.
embodiment 4
S1. by after the raw material blendings such as 10 parts of 70 parts, rice bran, 10 parts of dregs of beans, 10 parts of peanut press pulps, 10 parts, bean dregs and Semen Maydis powder mixture, by mixture tiling, preferably the thickness of tiling is 20cm; Press the mixture that 1Kg:0.1L inosine mother liquor nutritive medium soaks tiling, add yeast (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is AS2.180, and add-on is 7L), 60～68 ℃ of fermentations;
Ferment, after 5～7 days, mixture is carried out to turning, every turning in 1～3 month once, in turning, add the inedible oil fat (selecting food waste oil, the remaining waste oil in the kitchen, each hotel of originating) that accounts for mixture quality 2%, the moistening thickness of material; Continuing fermentation 5～6 months, mixture slaking is decomposed;
The temperature of the mixture after S2.S1 has fermented, slaking having been decomposed is down to 26～30 ℃, gross weight according to mixture: the volume of microbial nutrient solution is that 10Kg:2L adds microbial nutrient solution (preparation method is with embodiment 1), in the mixture that microorganism in microbial nutrient solution is decomposed in slaking, breed 10～20 days, obtain biological organic fertilizer of the present invention.After dry, be powdery, outward appearance is pitch-black glossy, has soy sauce fragrance, machinery-free impurity.
The bio-organic fertilizer that embodiment 1～4 is made detects through microbiological analysis inspection center of Guangdong Province and fertilizer quality supervision and inspection center of the Ministry of Agriculture, and the result of gained is all better than national standard, is shown in Table 1.
Table 1 bio-organic fertilizer detected result of the present invention
Wherein the validity period of embodiment 3 and embodiment 4 reaches 30 months.
5 kinds of embodiment plant the application experiment of cabbage heart
1. test site: test is carried out in the great Dong of Fengcheng neighbourhood committee food market, Shaoguan City of Guangdong Province Xinfeng County, and experimental field soil property is silty loam, middle fertility, preceding crop is fresh kidney beans.
2. test kind: cabbage heart late.
3. for examination fertilizer: biological organic fertilizer of the present invention, living bacteria count is not less than 0.3 hundred million cuf/g, and organic matter is not less than 47%, N+P
2o
5+ K
2o≤4%.
4. 4 processing are established in test, and each processing repeats for 3 times, random alignment, community area 20m
2.Cabbage heart is in sowing on December 30, and transplant January 30 next year, and mu is planted 2.5 ten thousand strains, and March 22, results were complete.Each Treatment Design is as follows:
Process one, before cabbage heart transplanting, mu is executed 80kg biological organic fertilizer of the present invention (biological organic fertilizer of selecting the embodiment of the present invention 1 to prepare is used) and is made base manure, topdresses: February 6 and February 19, biological organic fertilizer 10kg/ mu was executed in each pouring; Transplant latter 10 days every mu of points and execute three element compound fertilizer (15-15-15) 15kg, transplant latter 25 days every mu of points and execute three element compound fertilizer (15-15-15) 25kg.
Process two, cabbage heart is transplanted the front mu of biological organic fertilizer of executing 80kg deactivation (deactivation of Autoclave inner high voltage); Topdress: February 6 and February 19, the biological organic fertilizer 10kg/ mu of deactivation was executed in each pouring; Transplant latter 10 days every mu of points and execute three element compound fertilizer (15-15-15) 15kg, transplant latter 25 days every mu of points and execute three element compound fertilizer (15-15-15) 25kg.
Process three, the front mu of cabbage heart transplanting executes well-rotted farmyard manure 500kg and three element compound fertilizer (15-15-15) 25kg makees base manure, after mixing, spreads fertilizer over the fields, and the situation of topdressing is; Topdress: drench on February 6 and February 19 and execute urea 10kg/ mu, it is 1:100 that concentration is executed in pouring for the first time, and it is 1:50 that concentration is executed in pouring for the second time; Transplant latter 10 days every mu of points and execute three element compound fertilizer (15-15-15) 15kg, transplant latter 25 days every mu of points and execute three element compound fertilizer (15-15-15) 25kg.
Process four, blank (the full phase is not executed any fertilizer).
5. test-results:
(1) use the impact of biological organic fertilizer of the present invention on Growth of Brassica Parachinensis
In process of the test, observe, use the processing one of biological organic fertilizer of the present invention, cabbage heart leaf look dark green, and growing way is good, and blade is compared with wide and thick, tender flower stalk is thicker, as can be seen from Table 2, the plant height increasing degree of processing a cabbage heart is 6.2%, 88.4% and 73.1%, and width of blade increasing degree is respectively 13.3%, 76.5% and 103.3%, blade length increasing degree is respectively 11.5%, 89.7% and 56.4%, and the thick increasing degree of stem is respectively 0.0%, 66.7% and 80.0%; The plant height increasing degree of processing two cabbage hearts is 3.3%, 77.4% and 68.2%, width of blade increasing degree is respectively 7.1%, 66.7% and 96.0%, blade length increasing degree is respectively 8.3%, 84.6% and 54.2%, and the thick increasing degree of stem is respectively 0.0%, 50.0% and 66.7%; The plant height increasing degree of processing three cabbage hearts is 6.1%, 87.1% and 72.5%, width of blade increasing degree is respectively 12.5%, 77.8% and 96.9%, blade length increasing degree is respectively 12.0%, 89.3% and 56.6%, and the thick increasing degree of stem is respectively 0.0%, 66.7% and 60.0%; The plant height increasing degree of processing four cabbage hearts is 3.6%, 70.6% and 66.7%, width of blade increasing degree is respectively 7.7%, 64.3% and 91.3%, blade length increasing degree is respectively 4.6%, 78.3% and 50.5%, and the thick increasing degree of stem is respectively 0.0%, 50.0% and 33.3%.When results, process a cabbage heart plant height, width of blade, length and stem rough segmentation not than the increase by 21.6%, 24.5%, 16.2% and 80.0% of processing two, plant height and width of blade are respectively than the minimizing 0.4%, 3.1% of processing three, and blade length and stem rough segmentation be not than the increase by 3.6%, 12.5% of processing three; Respectively than the increase by 36.4%, 38.6%, 36.5% and 125.0% of processing four.Presentation of results, compares with the blank of using the biological organic fertilizer of deactivation and do not apply fertilizer, uses biological organic fertilizer of the present invention and can promote Growth of Brassica Parachinensis, increases cabbage heart plant height, width of blade, length and stem thick.
Table 2 is used the impact (unit: cm) of biological organic fertilizer of the present invention on Growth of Brassica Parachinensis
(2) use the impact of biological organic fertilizer of the present invention on yield of flowering Chinese cabbage
As can be seen from Table 3, between processing one and processing two, volume variance is remarkable, and between processing three, yield of flowering Chinese cabbage difference is not remarkable, and between processing four, poor heteropole is remarkable.Process an output the highest, process three and take second place, the 3rd of processing two, processes four output minimum.
The processing one community mean yield that cabbage heart is used biological organic fertilizer of the present invention is 28.7kg, folding per mu yield 956.7kg, and than processing two mu of volume increase 50.0kg, amplification is 5.5%, increase yield significantly; Than processing three mu of volume increase 6.7kg, amplification is 0.7%; Than processing four mu of volume increase 190.0kg, amplification is 24.8%, increases production extremely remarkable.Presentation of results, compares with processing two, three, four, and it is obvious that cabbage heart is used biological organic fertilizer production-increasing function of the present invention, contrasts with the conventional processing three of habits fertilization, and the two output approaches and slightly volume increase.
Table 3 is used the impact of biological organic fertilizer of the present invention on yield of flowering Chinese cabbage
After data in table, different lowercases are test significant difference through LSR, and different capitalizations are extremely remarkable through LSR test difference.
Conclusion (of pressure testing): with use the biological organic fertilizer of deactivation and compare with the blank not applying fertilizer, use biological organic fertilizer of the present invention and can promote Growth of Brassica Parachinensis, increase cabbage heart plant height, width of blade, length and stem thick, compared with the control, use biological organic fertilizer of the present invention and can promote cabbage heart volume increase.
6 kinds of embodiment plant the application experiment of cabbage heart
1. test site: test carry out in Red Star peasant household of Liao Tang village, river and mountain town, Fengkai County, Zhaoqing City of Guangdong Province vegetable plot, experimental field in fertility under, soil property silty loam, preceding crop is Lettuce.
2. test kind: blue or green No. 6 cabbage hearts of oil.
3. for examination fertilizer: biological organic fertilizer of the present invention, living bacteria count is not less than 0.3 hundred million cuf/g, and organic matter is not less than 47%, N+P
2o
5+ K
2o≤4%.
4. 4 processing are established in test, and each processing repeats for 3 times, random alignment, community area 20m
2.Cabbage heart is in sowing on February 10, and March 2 transplanted, and mu is planted 2.5 ten thousand strains, and April 6 gathered in the crops, and April 8, results were complete.Each Treatment Design is as follows:
Process one, before cabbage heart transplanting, (March 2) mu is executed 80kg biological organic fertilizer of the present invention (biological organic fertilizer of selecting the embodiment of the present invention 2 to prepare is used) and is made base manure, topdresses: March 9 and March 22, each pouring executed biological organic fertilizer 10kg/ mu; Transplant latter 10 days every mu of points and execute domestic composite fertilizer (15-15-15) 15kg, transplant latter 25 days every mu of points and execute domestic composite fertilizer (15-15-15) 25kg.
Process two, cabbage heart is executed the biological organic fertilizer of 80kg deactivation (deactivation of Autoclave inner high voltage) on (March 2) mu before transplanting; Topdress: March 9 and March 22, the biological organic fertilizer 10kg/ mu of deactivation was executed in each pouring; Transplant latter 10 days every mu of points and execute domestic composite fertilizer (15-15-15) 15kg, transplant latter 25 days every mu of points and execute domestic composite fertilizer (15-15-15) 25kg.
Process three, cabbage heart transplant before (March 2) mu execute well-rotted farmyard manure 500kg and domestic composite fertilizer (15-15-15) 50kg makees base manure, after mixing, spread fertilizer over the fields, the situation of topdressing is; Topdress: drench on March 9 and March 22 and execute urea 10kg/ mu, it is 1:100 that concentration is executed in pouring for the first time, and it is 1:50 that concentration is executed in pouring for the second time; Transplant latter 10 days every mu of points and execute domestic composite fertilizer (15-15-15) 15kg, transplant latter 25 days every mu of points and execute domestic composite fertilizer (15-15-15) 25kg.
Process four, blank (the full phase is not executed any fertilizer).
5. test-results:
(1) use the impact of biological organic fertilizer of the present invention on Growth of Brassica Parachinensis
In process of the test, observe, use the processing one of biological organic fertilizer of the present invention, cabbage heart leaf look dark green, and growing way is good, and blade is compared with wide and thick, tender flower stalk is thicker, data presentation from table 4 every 7 days, the plant height increasing degree of processing a cabbage heart is 56.3%, 69.3% and 85.8%, width of blade increasing degree is respectively 46.4%, 78.0% and 32.9%, blade length increasing degree is respectively 48.6%, 63.5% and 89.4%, and the thick increasing degree of stem is respectively 66.7%, 80.0% and 88.9%; The plant height increasing degree of processing two cabbage hearts is 54.2%, 67.6% and 84.7%, width of blade increasing degree is respectively 42.9%, 75.0% and 31.4%, blade length increasing degree is respectively 45.7%, 62.7% and 86.7%, and the thick increasing degree of stem is respectively 33.3%, 75.0% and 85.7%; The plant height increasing degree of processing three cabbage hearts is 50.0%, 66.7% and 82.5%, width of blade increasing degree is respectively 35.7%, 71.7% and 29.2%, blade length increasing degree is respectively 40.0%, 53.1% and 85.3%, and the thick increasing degree of stem is respectively 33.3%, 50.0% and 83.3%; The plant height increasing degree of processing four cabbage hearts is 47.9%, 66.2% and 74.6%, width of blade increasing degree is respectively 32.17%, 70.3% and 27.0%, blade length increasing degree is respectively 34.3%, 51.1% and 84.5%, and the thick increasing degree of stem is respectively 33.3%, 25.0% and 60.0%.
When results, process a cabbage heart plant height, width of blade, length and stem rough segmentation not than the increase by 3.1%, 5.4%, 3.9% and 30.8% of processing two, than the increase by 7.8%, 15.5%, 15.8%, 54.5% of processing three; Than the increase by 14.6%, 21.3%, 22.9% and 112.5% of processing four.Presentation of results, uses biological organic fertilizer of the present invention and can promote Growth of Brassica Parachinensis, increases cabbage heart plant height, width of blade, length and stem thick.
Table 4 is used the impact (unit: cm) of biological organic fertilizer of the present invention on Growth of Brassica Parachinensis
(2) use the impact of biological organic fertilizer of the present invention on yield of flowering Chinese cabbage
As can be seen from Table 5, between processing one and processing two, processing three, processing four, volume variance reaches utmost point conspicuous level.Process an output the highest, process two, three and take second place, process four output minimum.
The processing one community mean yield that cabbage heart is used biological organic fertilizer of the present invention is 50.4kg, folding per mu yield 1680.0kg, and than processing two mu of volume increase 120.0kg, amplification is 7.7%, increases production extremely remarkable; Than processing three mu of volume increase 253.3kg, amplification is 17.8%, increases production extremely remarkable; Than processing four mu of volume increase 283.3kg, amplification is 20.3%, increases production extremely remarkable.Presentation of results, compares with processing two, three, four, and it is obvious that cabbage heart is used biological organic fertilizer production-increasing function of the present invention.
Table 5 is used the impact of biological organic fertilizer of the present invention on yield of flowering Chinese cabbage
After data in table, different lowercases are test significant difference through LSR, and different capitalizations are extremely remarkable through LSR test difference.
Conclusion (of pressure testing): with use the biological organic fertilizer of deactivation and compare with the blank not applying fertilizer, use biological organic fertilizer of the present invention and can promote Growth of Brassica Parachinensis, increase cabbage heart plant height, width of blade, length and stem thick, compared with the control, use biological organic fertilizer of the present invention and can promote cabbage heart volume increase.
7 kinds of embodiment plant the application experiment of shatian pomelo
1. test site: test, in old wind gap reservoir orchard, Taihe county village, east of a river town, Wuhua County of Guangdong Province, is experimental field mountain red earth.
2. test kind: shatian pomelo.
3. for examination fertilizer: the biological organic fertilizer that the embodiment of the present invention 3 prepares, living bacteria count is not less than 0.3 hundred million cuf/g, You Ji Zhi≤30%, N+P
2o
5+ K
2o≤4%.
4. 4 processing are established in test, and each processing repeats for 3 times, random alignment, and each community selects the Shatian Pomelo strain that 4 strain growing ways are close to test.Each Treatment Design following (specifically with reference to embodiment 5 Treatment Design):
Process one, use biological organic fertilizer+custom and topdress;
Process two, use the biological organic fertilizer+custom of deactivation and topdress;
Process three, conventional contrast (custom base manure+custom is topdressed);
Process four, blank (the full phase is not executed any fertilizer).
Shatian pomelo is planted in spring sowing in 1998, mu tree planting approximately 25 strains, and on November 15th, 2011 gathers in the crops.Each processing fertilising situation is as follows: process one and make short fertilizers for potted flowers in ditch spread 4.0kg on March 5 in 2011 biological organic fertilizer/strain of the present invention and 1.0kg45% compound potassium sulfate fertilizer (18-10-17)/strain, 1.0kg biological organic fertilizer/strain of the present invention is executed in pouring on April 8 and 0.5kg compound potassium sulfate fertilizer (18-10-17) thanks to fertilizers for potted flowers, the phase of bearing fruit May 18 is used 1.0kg biological organic fertilizer/strain of the present invention and 2.0kg compound potassium sulfate fertilizer (18-10-17)/strain, before fruit picking on October 9, use 1.0kg biological organic fertilizer/strain of the present invention, after fruit picking on November 27, use 2.0kg biological organic fertilizer/strain of the present invention and 2.0kg compound potassium sulfate fertilizer (18-10-17)/strain, process two and use the biological organic fertilizer of deactivation one same period with processing, process three and use the peanut press pulp that becomes thoroughly decomposed a same period with processing, the four full phases of processing are not used any fertilizer.
5. test-results:
(1) use the impact of biological organic fertilizer of the present invention on shatian pomelo Main Agronomic shape
As seen from Table 6, process one and bear fruit number respectively than processing two, three, four increases by 6.6%, 2.1%, 13.3%; When results, processing a fruit footpath increases by 3.9%, 5.4%, 11.7% than processing two, three, four respectively; Single fruit Mean Fruit Weight increases by 5.9%, 5.1%, 13.0% than processing two, three, four respectively; Soluble solid increases by 0.9%, 0.3%, 2.0% than processing two, three, four respectively.Presentation of results, that uses that biological organic fertilizer of the present invention can increase shatian pomelo bears fruit number and single fruit on average heavily etc.
Table 6 is used the impact of biological organic fertilizer of the present invention on shatian pomelo Other Main Agronomic Characters
(2) use the impact of biological organic fertilizer of the present invention on shatian pomelo output
From table 7, process difference between a shatian pomelo output and processing two, three, four cell productions and reach conspicuous level.Process a shatian pomelo output the highest, process three and two and take second place, process four output minimum.The processing one shatian pomelo community mean yield of using biological organic fertilizer of the present invention is 475.4kg, and folding per mu yield 2859.4kg increases 327.5kg than processing two mu, and amplification 12.9%, increases production extremely remarkable; Increase 194.4kg, amplification 7.3%, increase yield significantly than processing three; Increase 626.3kg than processing four mu, amplification 28.0%, increases production extremely remarkable.Presentation of results biological organic fertilizer of the present invention has more significant production-increasing function to shatian pomelo.
Table 7 is used the impact of biological organic fertilizer of the present invention on shatian pomelo output
After data in table, different lowercases are test significant difference through LSR, and different capitalizations are extremely remarkable through LSR test difference.
Conclusion (of pressure testing): with use the biological organic fertilizer of deactivation and compare with the blank not applying fertilizer, use biological organic fertilizer of the present invention can increase shatian pomelo bear fruit number and single fruit on average weigh, contrast and compare with the routine of habits fertilization, use that biological organic fertilizer of the present invention can increase shatian pomelo fruit footpath and single fruit is on average heavy, shatian pomelo mu increase yield significantly.
8 kinds of embodiment plant the application experiment of shatian pomelo
1. test site: test is carried out in orchard, village, southwest, stone fan town, Mei County, Guangdong Province, is experimental field red soil, middle fertility.
2. test kind: shatian pomelo.
3. for examination fertilizer: biological organic fertilizer of the present invention, living bacteria count is not less than 0.3 hundred million cuf/g, You Ji Zhi≤30%, N+P
2o
5+ K
2o≤4%.
4. 4 processing are established in test, and each processing repeats for 3 times, random alignment, and each community selects the Shatian Pomelo strain that 4 strain growing ways are close to test.Each Treatment Design following (specifically with reference to embodiment 5 Treatment Design):
Process one, use biological organic fertilizer+custom and topdress;
Process two, use the biological organic fertilizer+custom of deactivation and topdress;
Process three, conventional contrast (custom base manure+custom is topdressed);
Process four, blank (the full phase is not executed any fertilizer).
Shatian pomelo was planted on March 15th, 1994, mu tree planting approximately 22 strains, and on November 6th, 2011 gathers in the crops.Each to process fertilising situation as follows: all execute 9.1kg fertilizer pig manure and 1.7kg potassium sulfate type Sa Kefu composite fertilizer (17-17-17) makes base manure in strain on November 23 in 2010, custom is topdressed points for 5 times.Wherein 0.5kg urea and the short fertilizers for potted flowers of 0.5kg potassium sulfate type Sa Kefu composite fertilizer (17-17-17) work are executed in strain on January 25 in 2011, 0.5kg urea is executed in strain on April 18 in 2011 and 1.0kg potassium sulfate type Sa Kefu composite fertilizer (17-17-17) makes steady fruit fertilizer, strain on May 16 in 2011 is executed 1.0kg potassium sulfate type Sa Kefu composite fertilizer (17-17-17) and is made fruit-promoting fertilizer, strain on June 25 in 2011 is executed 1.0kg potassium sulfate type Sa Kefu composite fertilizer (17-17-17) and is made fruit-promoting fertilizer, 1.0kg potassium sulfate type Sa Kefu composite fertilizer (17-17-17) is executed in strain on July 28 in 2011 and 0.5kg potassium sulfate is made fruit-promoting fertilizer.
The full phase of processing is used 9.0kg biological organic fertilizer/strain altogether, short fertilizers for potted flowers is made in wherein ditch spread 4.0kg on January 25th, 2011 biological organic fertilizer of the present invention/strain, pouring on April 18 is executed 1.0kg biological organic fertilizer/strain of the present invention and is thanked to fertilizers for potted flowers, May 16, the each pouring of fruit expanding period on June 25 are executed 1.0kg biological organic fertilizer/strain of the present invention and 2.0kg biological organic fertilizer/strain of the present invention, and fruit picking on September 25 is opened shallow ridges for first 40 days and used 1.0kg biological organic fertilizer/strain of the present invention; Process two and use the biological organic fertilizer of deactivation one same period with processing; Processing three uses base manure and topdresses according to locality custom; The four full phases of processing are not used any fertilizer.
5. test-results:
(1) use the impact of biological organic fertilizer of the present invention on shatian pomelo Main Agronomic shape
Process of the test is observed, and it is dark green that shatian pomelo is used biological organic fertilizer posterior lobe look of the present invention, and blade is thicker, falls leaves less.As seen from Table 8, after processing one flower thinning, female flower number increases by 1.3%, 1.2%, 2.6% than processing two, three, four respectively; The number of bearing fruit increases by 2.8%, 8.0%, 129.0% than processing two, three, four respectively; When results, processing a fruit footpath than processing two, four increases by 4.3%, 65.5%, reduces 1.4% than processing three; Single fruit on average increases by 8.3%, 4.0%, 73.3% than processing two, three, four heavily respectively.Soluble solid improves 0.6%, 0.2%, 4.3% than processing two, three, four respectively.Presentation of results, uses the single fruit that biological organic fertilizer of the present invention can increase shatian pomelo on average heavy, compares with the routine of habits fertilization, and using biological organic fertilizer of the present invention can increase the number of bearing fruit.
Table 8 is used the impact of biological organic fertilizer of the present invention on shatian pomelo Other Main Agronomic Characters
(2) use the impact of biological organic fertilizer of the present invention on shatian pomelo output
From table 9, process difference between a shatian pomelo output and processing two, three, four cell productions and reach utmost point conspicuous level.Process a shatian pomelo output the highest, process two and three and take second place, process four output minimum.The processing one shatian pomelo community mean yield of using biological organic fertilizer of the present invention is 774.4kg, and folding per mu yield 4259.2kg increases 431.2kg than processing two mu, and amplification 11.3%, increases production extremely remarkable; Than processing three mu of volume increase 3186.7kg, amplification 297.1%, increases production extremely remarkable; Than processing four mu of volume increase 3186.7kg, amplification 297.1%, increases production extremely remarkable.Presentation of results biological organic fertilizer of the present invention has more significant production-increasing function to shatian pomelo.
Table 9 is used the impact of biological organic fertilizer of the present invention on shatian pomelo output
After data in table, different lowercases are test significant difference through LSR, and different capitalizations are extremely remarkable through LSR test difference.
Conclusion (of pressure testing): using biological organic fertilizer of the present invention can significantly increase shatian pomelo output.Compare with the blank not applying fertilizer with using the biological organic fertilizer of deactivation, using biological organic fertilizer of the present invention, can to increase shatian pomelo single fruit on average heavy, contrasts and compare with the routine of habits fertilization, and using biological organic fertilizer of the present invention can increase the shatian pomelo number of bearing fruit.
9 kinds of embodiment plant the application experiment of lichee
1. test site: test is carried out respectively in orchard, 4, Sha Dong area under control, Shi Li village, Guan Zhu town, Maoming Dianbai County.Be all low mountain, hills red earth.
2. test kind: lichee (white sugar small-mouthed jar).
3. for examination fertilizer: biological organic fertilizer of the present invention, living bacteria count is not less than 0.3 hundred million cuf/g, You Ji Zhi≤30%, N+P
2o
5+ K
2o≤4%.
4. test orchard situation is as shown in table 10:
Table 10
The average strain in orchard 1 is produced 18.75 kilograms, 468.8 kilograms of per mu yields, and strain is produced and is increased production 120% and 460% than orchard 3 and orchard 4 respectively, and per mu yield increases 87.5% and 300%; Orchard 2 strains are produced and are increased 77.8% and 344.7% than orchard 3 and orchard 4, and per mu yield increases 77.9% and 300% than orchard 3 and orchard 4.
the application experiment of embodiment 10 rice cultivations
Experimental plot is in field, Tang Dong roadside, Jiu Dui big city, Xin Tangxiang Shan Bian village, Guangzhou.Soil type is field at the bottom of pellet in a shotgun cartridge, and organic content is lower, and paddy rice is the excellent perfume (or spice) of spoke.
Experimental plot in August 8 rice transplanting mode transplant seedlings, establish 0.5 mu of test site, before rice transplanting, use 25 kilograms of biological organic fertilizers of the present invention, folding mu is executed 50 kilograms.0.52 mu of check plot area, executes 20 kilograms of carbon ammoniums, and folding mu is executed 38.5 kilograms, 30 kilograms, fused(calcium magnesium)phosphate, and folding mu is executed 59.6 kilograms.Between two processing, build ridge and be separated by, perpendicular board is expressed.Each district fertilizer keeps shoaling layer to spread down, harrows even rice transplanting.
Field management: keep shoaling layer to turn green after rice transplanting.August 16,5 kilograms, urea is executed in 0.5 mu of test site, executes 6.2 kilograms of import composite fertilizers (nitrogen 15, phosphorus 15, potassium 15); 0.52 mu of check plot, executes 5.2 kilograms, urea, 6.5 kilograms of import composite fertilizers.Each treatment zone folding mu is executed 10 kilograms, urea, and 12.5 kilograms of composite fertilizers do disposable topdressing and use sufficient.After enough seedlings water layer naturally fall dry, dry wet management to booting mid-term.Draw water for twice and solve heading water because of autumn drought afterwards.Insect pest preventing and controlling in August 28, September 11 and each integrated control on October 22 once.November 10 ripe and harvested, totally 105 days breeding time of Honda.
Before checking and accepting, invite peasant household to participate in stochastic sampling, one square metre of test site sampling, effectively fringe 312 fringes, effectively fringe 208010 fringes of folding mu; One square metre of check plot stochastic sampling, effectively fringe 309 fringes, effectively fringe 206010 fringes of folding mu.The harvesting on November 10 of each treatment zone, is participated in by peasant household and the villager that passes by one's way, and 10 square metres of stochastic sampling harvestings are as shown in table 11 below:
Table 11 is used biological organic fertilizer experimental result of the present invention
Test Summary: the test site seedlings of cereal crops are just showed than check plot viridescent for the 12nd day after rice transplanting, fertility is fast, and precocious 2～3 days, and during being continued until Heading filling.Biological organic fertilizer of the present invention, fertilizer has soy sauce fragrance, not smelly, and peasant household likes using, and late rice is used and obtains 33.3 kilograms of mu volume increase, and amplification is 7.03%, benefit is very remarkable; After using fertilizer of the present invention, the soil organism has a certain amount of increase, and arable soil colloid and crumb structure are played a protective role.Use biological organic fertilizer of the present invention, can effectively reduce the pollution to arable land of chemical fertilizer, adhere to life-time service, can progressively repair the soil of degenerating because of long-term single use chemical fertilizer.
Comparative example 1
By bacillus megaterium (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is ACCC11011), silicate bacteria is (purchased from Agricultural University Of South China, Article Number is IM6), vinelandii are (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is AS1.222) render in inosine mother liquor nutritive medium and cultivate, make to contain in every milliliter of microbial nutrient solution: phosphate solubilizing bacteria 0.1 hundred million cuf/mL, potassium solubilizing bacteria 0.1 hundred million cuf/mL, vinelandii 0.1 hundred million cuf/mL.Cultivation obtains the microbial nutrient solution that contains various trace elements for 50～60 days at 25～30 ℃, (not need camera bellows).Above-mentioned bacterial strains is not limited to described purchasing channel, can be also self-control or other sources.Inosine mother liquor nutritive medium mainly contains organic matter, nitrogen, phosphorus, potassium, humic acid and glucose.
Prepare biological organic fertilizer step as follows:
S1. by 50 parts, rice bran, 20 parts of dregs of beans, after 20 parts of raw material blendings such as grade of 10 parts of peanut press pulps and Semen Maydis powder, obtain mixture, mixture is tiled, preferably the thickness of tiling is 10cm, press the mixture (guaranteeing that the mixed material of liquid surrounds) that 1Kg:0.1L inosine mother liquor nutritive medium soaks tiling, in mixture, add yeast (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is AS2.180, add-on is 6L), be maintained at 55～60 ℃ of fermentations, ferment, after 5～7 days, starting material are carried out to turning, later every turning in 1～3 month 1 time, continuing fermentation 5～6 months, mixture slaking is decomposed,
The temperature of the mixture after having fermented described in S2.S1, slaking having been decomposed is down to 26～30 ℃, gross weight according to mixture: the volume of microbial nutrient solution is that 10Kg:2L adds microbial nutrient solution, in the mixture that microorganism in microbial nutrient solution is decomposed in slaking, breed 10～20 days, obtain biological organic fertilizer.
comparative example 2
By bacillus megaterium (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is ACCC11011), silicate bacteria is (purchased from Agricultural University Of South China, Article Number is IM6), vinelandii are (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is AS1.222) render in inosine mother liquor nutritive medium and cultivate, make to contain in every milliliter of microbial nutrient solution: phosphate solubilizing bacteria 0.1 hundred million cuf/mL, potassium solubilizing bacteria 0.1 hundred million cuf/mL, vinelandii 0.1 hundred million cuf/mL.Cultivation obtains the microbial nutrient solution that contains various trace elements for 50～60 days at 25～30 ℃, (not need camera bellows).Above-mentioned bacterial strains is not limited to described purchasing channel, can be also self-control or other sources.Inosine mother liquor nutritive medium mainly contains organic matter, nitrogen, phosphorus, potassium, humic acid and glucose.
Prepare biological organic fertilizer step as follows:
S1. by 90 parts, rice bran, 5 parts of dregs of beans, after 5 parts of raw material blendings such as grade of 5 parts of peanut press pulps and bean dregs, obtain mixture, mixture is tiled, preferably the thickness of tiling is 10cm, press the mixture (guaranteeing that the mixed material of liquid surrounds) that 1Kg:0.1L inosine mother liquor nutritive medium soaks tiling, in mixture, add yeast (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is AS2.180, add-on is 6L), be maintained at 55～60 ℃ of fermentations, ferment, after 5～7 days, starting material are carried out to turning, later every turning in 1～3 month 1 time, continuing fermentation 5～6 months, mixture slaking is decomposed,
The temperature of the mixture after having fermented described in S2.S1, slaking having been decomposed is down to 26～30 ℃, gross weight according to mixture: the volume of microbial nutrient solution is that 10Kg:2L adds microbial nutrient solution, in the mixture that microorganism in microbial nutrient solution is decomposed in slaking, breed 10～20 days, obtain biological organic fertilizer.
comparative example 3
By bacillus megaterium (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is ACCC11011), silicate bacteria is (purchased from Agricultural University Of South China, Article Number is IM6), vinelandii are (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is AS1.222) render in inosine mother liquor nutritive medium and cultivate, make to contain in every milliliter of microbial nutrient solution: phosphate solubilizing bacteria 0.05 hundred million cuf/mL, potassium solubilizing bacteria 0.05 hundred million cuf/mL, vinelandii 0.05 hundred million cuf/mL.Cultivation obtains microbial nutrient solution for 30 days at 25～30 ℃, (not need camera bellows).Above-mentioned bacterial strains is not limited to described purchasing channel, can be also self-control or other sources.Inosine mother liquor nutritive medium mainly contains organic matter, nitrogen, phosphorus, potassium, humic acid and glucose.
Prepare biological organic fertilizer step as follows:
S1. by after the raw material blendings such as 5 parts, 60 parts, rice bran, 5 parts of dregs of beans, 5 parts of peanut press pulps and bean dregs mixture, mixture is tiled, preferably the thickness of tiling is 10cm, press the mixture (guaranteeing that the mixed material of liquid surrounds) that 1Kg:0.05L inosine mother liquor nutritive medium soaks tiling, in mixture, add yeast (purchased from microbiological analysis inspection center of Guangdong Province, Article Number is AS2.180, add-on is 6L), be maintained at 55～60 ℃ of fermentations, ferment, after 3 days, starting material are carried out to turning, continuing fermentation 5～6 months, decomposes mixture slaking;
The temperature of the mixture after having fermented described in S2.S1, slaking having been decomposed is down to 40 ℃, gross weight according to mixture: the volume of microbial nutrient solution is that 10Kg:1L adds microbial nutrient solution, in the mixture that microorganism in microbial nutrient solution is decomposed in slaking, breed 10～20 days, obtain biological organic fertilizer.
The fertilizer that comparative example 4 comparative examples prepare and fertilizer of the present invention are the application contrast experiment of plantation cabbage heart
1. test site: test is carried out in the great Dong of Fengcheng neighbourhood committee food market, Shaoguan City of Guangdong Province Xinfeng County, and experimental field soil property is silty loam, middle fertility, preceding crop is fresh kidney beans.
2. test kind: cabbage heart late.
3. for examination fertilizer: biological organic fertilizer of the present invention, living bacteria count is not less than 0.3 hundred million cuf/g, and organic matter is not less than 47%, N+P
2o
5+ K
2o≤4%.
4. 4 processing are established in test, and each processing repeats for 3 times, random alignment, community area 20m
2.Cabbage heart is in sowing on December 30, and transplant January 30 next year, and mu is planted 2.5 ten thousand strains, and March 22, results were complete.Each Treatment Design is as follows:
Process one, before cabbage heart transplanting, mu is executed 80kg biological organic fertilizer of the present invention (biological organic fertilizer of selecting the embodiment of the present invention 1 to prepare is used) and is made base manure, topdresses: February 6 and February 19, biological organic fertilizer 10kg/ mu was executed in each pouring; Transplant latter 10 days every mu of points and execute three element compound fertilizer (15-15-15) 15kg, transplant latter 25 days every mu of points and execute three element compound fertilizer (15-15-15) 25kg.
Process two, before cabbage heart transplanting, mu is executed 80kg biological organic fertilizer of the present invention (biological organic fertilizer of selecting comparative example 1 to prepare is used) and is made base manure, topdresses: February 6 and February 19, the biological organic fertilizer 10kg/ mu that ratio 1 prepares was executed in each pouring; Transplant latter 10 days every mu of points and execute three element compound fertilizer (15-15-15) 15kg, transplant latter 25 days every mu of points and execute three element compound fertilizer (15-15-15) 25kg.
Process three, before cabbage heart transplanting, mu is executed 80kg biological organic fertilizer of the present invention (biological organic fertilizer of selecting comparative example 2 to prepare is used) and is made base manure, topdresses: February 6 and February 19, the biological organic fertilizer 10kg/ mu that comparative example 2 prepares was executed in each pouring; Transplant latter 10 days every mu of points and execute three element compound fertilizer (15-15-15) 15kg, transplant latter 25 days every mu of points and execute three element compound fertilizer (15-15-15) 25kg.
Process four, before cabbage heart transplanting, mu is executed 80kg biological organic fertilizer of the present invention (biological organic fertilizer of selecting comparative example 3 to prepare is used) and is made base manure, topdresses: February 6 and February 19, the biological organic fertilizer 10kg/ mu that comparative example 3 prepares was executed in each pouring.
Process five, blank (the full phase is not executed any fertilizer).
5. test-results:
From table 12, each stage cabbage heart plant height, width of blade, length and the thick data of stem can be found out, the fertilizer efficiency of biological organic fertilizer of the present invention is well more a lot of than the fertilizer efficiency of the standby biological organic fertilizer obtaining of comparative example.
Table 12 is used biological organic fertilizer of the present invention and the impact (unit: cm) of comparative example biological organic fertilizer on Growth of Brassica Parachinensis
Claims (9)
1. a preparation method for biological organic fertilizer, is characterized in that, comprises the following steps:
S1. rice bran, dregs of beans, peanut press pulp and bean dregs are mixed in proportion to obtain to mixture, by mixture tiling, with the mixture after inosine mother liquor nutritive medium immersion tiling, add yeast, in 50～60 ℃ of bottom fermentations,
In fermenting process, carry out turning processing;
After having fermented described in S2.S1, the temperature of mixture is down to 26～30 ℃, adds microbial nutrient solution, the microorganism in microbial nutrient solution breeds 10～20 days in mixture, obtains described biological organic fertilizer; Wherein, described in S1, in mixture, rice bran, dregs of beans, peanut press pulp and bean dregs parts by weight are: rice bran 60～80 weight parts, dregs of beans 5～15 weight parts, peanut press pulp 5～15 weight parts, bean dregs 5～15 weight parts;
Described in S2, in microbial nutrient solution, contain phosphate solubilizing bacteria 0.1～0.3 hundred million cuf/mL, potassium solubilizing bacteria 0.1～0.3 hundred million cuf/mL and vinelandii 0.1～0.3 hundred million cuf/mL;
In microbial nutrient solution described in S2, described phosphate solubilizing bacteria is bacillus megaterium (Bacillus megaterium), and described potassium solubilizing bacteria is silicate bacteria (Silicate bacteria), and described vinelandii are vinelandii (Azotobacter sp).
2. the preparation method of biological organic fertilizer according to claim 1, is characterized in that described in S1, in fermenting process, adding the inedible oil fat that accounts for mixture quality 1.5～2% after 5th～7 days; Described inedible oil fat is glycerine or edible waste oil.
3. the preparation method of biological organic fertilizer according to claim 1, is characterized in that, the consumption of inosine mother liquor nutritive medium is according to the gross weight of mixture described in S1: the volume of inosine mother liquor nutritive medium is that 10Kg:1L determines; Described in S2, the consumption of microbial nutrient solution is according to the gross weight of mixture: the volume of microbial nutrient solution is that 10Kg:2L determines.
4. the preparation method of biological organic fertilizer according to claim 1, is characterized in that containing in the every mL microbial nutrient solution of S2: phosphate solubilizing bacteria 0.2 hundred million cuf, potassium solubilizing bacteria 0.2 hundred million cuf and vinelandii 0.2 hundred million cuf.
5. the preparation method of biological organic fertilizer according to claim 1, it is characterized in that, the preparation method of the microbial nutrient solution described in S2 is: bacillus megaterium, silicate bacteria, vinelandii are rendered in inosine mother liquor nutritive medium, cultivated 50～60 days and get final product in 20～30 ℃.
6. the preparation method of biological organic fertilizer according to claim 1, is characterized in that, turning processing is after 5～7 days, mixture to be carried out to turning for the first time in fermentation described in S1, after turning for the first time every turning in 1～3 month once; The time of described fermentation is 5～6 months.
7. the preparation method of biological organic fertilizer according to claim 6, is characterized in that, adds the inedible oil fat that accounts for mixture quality 1.5～2% described in S1 when fermentation was carried out turning for the first time to mixture after 5～7 days; Described inedible oil fat is glycerine or edible waste oil.
8. the biological organic fertilizer that described in claim 1 to 7 any one, preparation method prepares.
9. the application of biological organic fertilizer aspect plantation lichee, cabbage heart, shatian pomelo or paddy rice described in claim 8.
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|---|---|---|---|---|
| CN104725083A (en) * | 2013-12-19 | 2015-06-24 | 王骁 | Flower fertilizer and preparation method thereof |
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| CN1434004A (en) * | 2003-03-11 | 2003-08-06 | 赵鹏 | Biological organic-inorganic compound fertilizer and productive method thereof |
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