CN103130534B - Sawdust bacteria bran nutritional agent for culturing lavender and preparation method thereof - Google Patents
Sawdust bacteria bran nutritional agent for culturing lavender and preparation method thereof Download PDFInfo
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- CN103130534B CN103130534B CN201310098226.5A CN201310098226A CN103130534B CN 103130534 B CN103130534 B CN 103130534B CN 201310098226 A CN201310098226 A CN 201310098226A CN 103130534 B CN103130534 B CN 103130534B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a sawdust bacteria bran nutritional agent for culturing lavender and a preparation method thereof. The sawdust bacteria bran nutritional agent is prepared by inoculating bacillus subtilis on a mixture of sawdust bacteria bran and fresh mushroom stipe water extract and fermenting. The preparation method comprises the following steps: A, preparing a sawdust bacteria bran solid-state accumulation fermentation culture medium, compounding 93 parts of sawdust bacteria bran, 5 parts of bran and 2 parts of cane sugar, stirring materials by using fresh mushroom stipe, wherein the ratio of materials to water is 1:1.2-1.3; B, performing accumulation fermentation; and C, further compounding the fermented bacteria bran obtained in step B, compounding 70 parts of fermented sawdust bacteria bran, 15 parts of garden soil and 15 parts of sand, and obtaining the sawdust bacteria bran nutritional agent used for culturing the lavender. A novel implementation scheme is provided for resource development of edible mushroom industry wastes, and an optimized technical route is provided for large-scale culture of the economic plants such as lavender.
Description
Technical field
The present invention relates to a kind of nutrition agent that utilizes wood chip matter bacterium chaff cultivation fragrant ornamental plant lavandula angustifolia and preparation method thereof, belong to the biology exploitation of wood chip matter bacterium chaff in mushroom industry and the preparation method of plant growing material.
Background technology
China is Edible Fungi big country, edible mushrooms gross annual output amount approximately 1.8 × 10
7t, after the mushroom of gathering, remaining cultivation matrix is bacterium chaff, and the annual bacterium chaff producing of China is greatly about 4.57 × 10
7t left and right.Wood chip is that wooden rotten type edible mushrooms is as the main raw material in the cultivations such as mushroom, needle mushroom, auricularia auriculajudae, only taking mushroom as example, the mushroom production of China has accounted for 70% of world wide production, wood chip matter bacterium chaff output is huge, and the annual wood chip matter bacterium chaff producing is not effectively processed mostly, discarded wood chip matter bacterium chaff arbitrarily abandons, and causes the secondary pollution of agriculture pollution of area source or mushroom, or burns generation waste gas.Environment is impacted.At present, caused a series of environmental problem because wood chip matter bacterium chaff arbitrarily abandons, easily grown various microorganisms, become the environment living contaminants source of Edible Fungi around and even agriculture production, caused potential biology to threaten.What therefore solve wood chip matter bacterium chaff utilizes problem just significant.Although the most of nutrient in matrix is utilized, contained organic substance, carbon nitrogen nutrition and trace element element etc. are still very abundant.Wood chip matter planting material is after fruiting, although part matrix is decomposed, has absorbed a part of nutrition, but after the decomposition of edible mushrooms, produce again new mushroom secondary metabolite simultaneously, in wood chip matter bacterium chaff, also contain a large amount of mycelium and unemployed organism, after measured, the wood chip matter bacterium chaff taking wood chip after main raw material mushroom culture, their crude protein, crude fat, robust fibre, mineral substance and nitrogen-free extract are respectively 8.7%, 6.2%, 30.6%, 7.9%, 42.0%.
Lavandula angustifolia (Lavandula angustifolia), for Labiatae lavandula angustifolia belongs to perennial subshrub plant, originates in the area that Mediterranean rainfall is few, has been just quite general vanilla at Roman period; Have at present 37 kinds, more than 100 kind, point essential oil is used lavandula angustifolia two classes with lavandula angustifolia with viewing and admiring.At present there is good plantation DEVELOPMENT PROSPECT in China, originate as high added value spices.The narrow leaf lavandula angustifolia of main species, its cultivation needs slight alkalinity or neutral, well-drained medium particularly, physical property and the peculiar nutritive value of wood chip matter bacterium chaff is also aided with the yellow seriflux that lavandula angustifolia growth is had to promoter action, through preparation, can meet nutrition and the moisture requirement of its growth, raise the efficiency, also for the resource utilization exploitation of wood chip matter bacterium chaff and starch industry processing waste yellow seriflux provides new technological line.
In addition, most starches processing enterprise of China all directly discharges yellow seriflux, does not only make full use of its nutritive value, and directly environment has also been polluted in discharge.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of method of utilizing wood chip matter bacterium chaff cultivation lavandula angustifolia; for the resource utilization exploitation of mushroom industry waste provides a kind of brand-new embodiment; simultaneously for this edible large-scale planting that can medicine high added value economic plants of lavandula angustifolia provides a kind of technological line of optimization, realize green circulation economy pattern, improve ecological.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows.
For cultivating a wood chip bacterium chaff nutrition agent for lavandula angustifolia, it is on the mixture of wood chip bacterium chaff and fresh mushroom stem lixiviate water, inoculate subtilis and make through fermentation.
As a preferred technical solution of the present invention, in the fermentating formula of described wood chip bacterium chaff nutrition agent, also comprise wheat bran and/or sugar.
As a preferred technical solution of the present invention, in the batching of described wood chip bacterium chaff nutrition agent, also comprise garden soil and/or husky.
Prepare above-mentionedly for cultivating the method for wood chip bacterium chaff nutrition agent for lavandula angustifolia, its step comprises:
The preparation of A, the solid-state heap fermentation substratum of wood chip bacterium chaff: get by weight 2 parts of 93 parts of wood chip bacterium chaffs, 5 parts, wheat bran, sucrose and carry out compositely, use fresh mushroom stem lixiviate water spice, material-water ratio is 1:(1.2-1.3);
B, heap fermentation: on the substratum of steps A preparation, carry out bacillus subtilis microbial agent inoculation, build heap heap fermentation, timing turning, has fermented for 8-10 days; Gained zymophyte chaff product comprises the required various nutrients of lavandula angustifolia plant culture;
C, first step B gained fermentation wood chip matter bacterium chaff is adjusted to pH to 7 and then carried out further composite to fermentation wood chip matter bacterium chaff, getting by weight 15 parts, husky 15 parts of 70 parts of fermentation wood chip matter bacterium chaffs, garden soil carries out composite, obtain wood chip bacterium chaff nutrition agent, for the cultivation of lavandula angustifolia.
As a kind of optimal technical scheme of above-mentioned preparation method, in steps A, first the wood chip bacterium chaff using dries and is crushed to 0.4-0.6cm and sieves, then is used for carrying out the preparation of substratum.
As a kind of optimal technical scheme of above-mentioned preparation method, in steps A, the preparation method of fresh mushroom stem extracting solution is: 0.5%Nacl aqueous solution 1:10 proportioning by weight for the waste fresh mushroom stem of pruning, smash micro-boiling 10 minutes, 2 times, filter, merge to obtain filtrate spice.
As a kind of optimal technical scheme of above-mentioned preparation method, in steps A, use fresh mushroom stem lixiviate water spice, fresh mushroom stem lixiviate water consumption is controlled at the windrow hand seam water outlet degree of holding.
As a kind of optimal technical scheme of above-mentioned preparation method, while building heap heap fermentation in step B, building heap specification is 2m × 1.5m × 10m; Within in fermenting process three days, turn over once heap.
As a kind of optimal technical scheme of above-mentioned preparation method, in step C, the zymophyte chaff using first spread out be exposed to the sun 1-3 days and smash after be used for again carrying out composite.
As a kind of optimal technical scheme of above-mentioned preparation method, in step C, control gained wood chip bacterium chaff nutrition agent and have that draining is good, dry, neutral meta-alkali, the fertile cultivation feature of nutrient.
Adopt the beneficial effect that produces of technique scheme to be: the present invention has opened up the biological utilization methods of the wood chip matter bacterium chaff in implantation methods and the mushroom industry of a kind of lavandula angustifolia, in addition the compatibility utilization of waste fresh mushroom stem extracting solution, has comprehensively drawn the composite aftertreatment technology after pre-treatment formula before wood chip matter bacterium chaff heap fermentation, wood chip matter bacterium chaff are with operational path and the heap fermentation of fresh mushroom stem extracting solution spice heap fermentation.The resource utilization exploitation that the present invention is mushroom industry waste provides a kind of brand-new embodiment; simultaneously for this edible large-scale planting that can medicine high added value economic plants of lavandula angustifolia has been opened up a kind of technological line of optimization; really realize green circulation economy pattern, be all significant for existing planting edible mushroom industry, mushroom stem comprehensive utilization industry, lavandula angustifolia plantation industry!
In the present invention, the compatibility of the recycling of wood chip matter bacterium chaff, waste fresh mushroom stem extracting solution uses, and all meets the technical requirements of recycling economy, is not only conducive to the deep development of resource, and contributes to reduce environmental pollution.Wood chip matter bacterium chaff is prepared into lavender flower soil by the present invention, and the latter possesses very high nutritive property and water conservation ventilation property.The income that can increase flower-grower with this law cultivation lavandula angustifolia, can also transform agroforestry waste, reduces environmental pollution, significant for Economic development and ecological improvement.Method material of the present invention is easy to get, cost is low, economic benefit is high and ecological significance is great.
Beneficial effect of the present invention has been verified in following experimental study.
Test 1, the broiler diets comparative study of quick-acting carbon source sucrose in the solid-state heap fermentation of wood chip matter bacterium chaff
Although wood chip matter bacterium chaff itself is due to the existence of the material such as mycelium, secondary metabolite, nutritious, but be the Fast-propagation that improves heap fermentation efficiency, is more conducive to early stage subtilis in solid-state heap fermentation, on different carbon nitrogen source pilot studies basis, sucrose and wheat bran that design low value is easy to get are studied as proportioning material as quick-acting carbon sources and quick-acting carbon source.
Microbial inoculum subtilis living bacteria count (hundred million/g) >=100 used.Wood chip matter bacterium chaff is pulverized with Chinese medicine material crushing machine, crosses 60 mesh sieves for subsequent use.Weigh 93 grams and after 5 grams of wheat brans, add sucrose to be dissolved in clear water according to the weight of weight ratio 1,2,3,4 respectively, mix spice by 1:1.2 material-water ratio, after autoclaving 30 minutes, add with batch equivalent microbial inoculum by the bacterium amount that the connects aseptic technique of 10,000,000,000/g wood chip matter bacterium chaff, 37 DEG C of cultivations, conventional gradient dilution colony counting method inspection bacterium, the growing state of subtilis under evaluation different sugar concentration.Be contrast with sucrose group.
The growing state of subtilis under the different sucrose additions of table 1
Result shows, sucrose adds effectively, can be used as quick-acting carbon sources, before 48 hours, promoted subtilis determine grow, shorten its fermentation adaptive phase, substantially convergent after 5 days, therefore complex art and economic factors, sucrose addition is advisable with 2 parts of groups.
The use of test 2, waste fresh mushroom stem extracting solution
Waste fresh mushroom stem is many at present directly to be abandoned, but wherein also has a large amount of nutritive substances to utilize.This research and design clear water be the waste fresh mushroom stem extracting solution spice experiment of contrast.This experiment fresh mushroom stem is taken from teaching and scientific research practice base, our unit Laishui, and preparation method as previously mentioned; Soluble solid content 2%.
Microbial inoculum subtilis living bacteria count (hundred million/g) >=100 used.Wood chip matter bacterium chaff is pulverized with pulverizer, crosses 60 mesh sieves for subsequent use.Weigh 93 grams and mix with addition of 5 grams of wheat brans, 2 grams of sucrose after, press the material-water ratio fresh mushroom stem extracting solution spice of 1:1.2, after autoclaving 30 minutes, add with batch equivalent microbial inoculum by the bacterium amount that the connects aseptic technique of 10,000,000,000/g wood chip matter bacterium chaff, 37 DEG C of cultivations, conventional gradient dilution colony counting method inspection bacterium, the effect of evaluation fresh mushroom stem extracting solution.If clear water is contrast.
Table 2 fresh mushroom stem extracting solution adds the observation on Growth to subtilis
Fresh mushroom stem extracting solution due to still contain can for subtilis breeding, the nutrient such as protein, using it as spice water, contribute to the solid-state heap fermentation process of wood chip matter bacterium chaff in the present invention, make up the nutrient deficiency of wood chip matter bacterium chaff.
Test 3, wood chip matter wood chip bacterium chaff nutrition agent cultivation lavandula angustifolia growth contrast
With the wood chip matter bacterium chaff of preparation of the present invention, through combination and compatibility, be wood chip wood chip bacterium chaff nutrition agent.Wood chip matter bacterium chaff and river sand, all have the effect of the ventilation property that increases base-material, in the wood chip matter bacterium chaff after the present invention's preparation, contains abundant nitrogen phosphorus potassium, organism and trace element, is conducive to absorbing of plant.
Experiment arranges: process 1: common soil; Process 2: the wood chip matter bacterium chaff nutrition agent of clear water spice fermentation; Process 3: the wood chip matter wood chip bacterium chaff nutrition agent of fresh mushroom stem extracting solution spice fermentation; Process 4: commercially available Nutrition Soil; Treatment process: 50 seeds are got in every processing, should soak seed before sowing 12 hours, then within 2 hours, sows with the seed soaking of 20-50ppm Plant hormones regulators,gibberellins again.Culture medium waters permeable, after oozing, evenly broadcasts seed under water, then covers one deck fine earth, and thickness is 0.2 centimetre, covers grass or plastics film soil moisture conservation.At 18-22 DEG C of the optimum temperutures of seed germination, take out seedling strain 30 strains, within 3 days, start to observe sprouting situation, within 15 days, measure shoot fresh weight, calculating mean value.
The table 3 wood chip matter bacterium chaff nutrition agent cultivation lavandula angustifolia situation of emerging contrasts
Comparing result shows, wood chip bacterium chaff nutrition agent of the present invention can well be cultivated fragrant ornamental plant lavandula angustifolia, after fresh mushroom stem extracting solution spice heap fermentation, due to its abundant mushroom (macro fungi such as mushroom, needle mushroom; Subtilis) secondary metabolite, and the trace element containing in fresh mushroom stem extracting solution, wood chip matter bacterium chaff, show the promoter action to lavandula angustifolia plant development.
Embodiment
Following examples describe the present invention in detail.Various raw material used in the present invention and items of equipment are conventional commercially available prod, all can be bought directly and be obtained by market.
Embodiment 1, the cultivation of mushroom wood chip matter bacterium chaff
1, select wood chip matter bacterium chaff after sawdust mushroom cultivation to dry to be crushed to 0.4cm and be advisable;
2, get 93 parts of wood chip matter bacterium chaffs by quality proportioning: 5 parts, wheat bran, 2 parts of sucrose, use fresh mushroom stem extracting solution spice, material-water ratio is 1:1.2, can grasp to holding the water outlet of windrow hand seam and be advisable in production operation.Wherein, the preparation method of fresh mushroom stem extracting solution is: the waste fresh mushroom stem of pruning by weight 1:10 proportioning, is smashed micro-boiling 10 minutes with the 0.5%Nacl aqueous solution, 2 times, filter, and merge to obtain filtrate spice.
3, commercially available bacillus subtilis microbial agent inoculation, inoculum size is taking microbial inoculum service requirements as fixed, and routine is built heap heap fermentation, 2m × 1.5m × 10m.Within three days, turn over once heap, moisturizing in right amount in turning process.About 9 days, fermentation completes.While fermentation, present brownly, free from extraneous odour, without going mouldy.
4, fine day is spread fermentation heap to be exposed to the sun two days out, smashes or pulverizer pulverizing.Composite with reference to ratio below: fermentation wood chip matter bacterium chaff: garden soil: river sand: 70%, 15%, 15%, make lavandula angustifolia wood chip matter bacterium chaff nutrition cultivation matrix.
5, conventional lavandula angustifolia cultivating and growing management.
Embodiment 2, the cultivation of needle mushroom wood chip matter bacterium chaff
1, select wood chip matter bacterium chaff after cultivation on sawdust needle mushroom to dry to be crushed to 0.5cm and be advisable;
2, get 93 parts of wood chip matter bacterium chaffs by quality proportioning: 5 parts, wheat bran, 2 parts of sucrose, use fresh mushroom stem extracting solution spice, material-water ratio is 1:1.3, can grasp to holding the water outlet of windrow hand seam and be advisable in production operation.Wherein, the preparation method of fresh mushroom stem extracting solution is: the waste fresh mushroom stem of pruning by weight 1:10 proportioning, is smashed micro-boiling 10 minutes with the 0.5%Nacl aqueous solution, 2 times, filter, and merge to obtain filtrate spice.
3, commercially available bacillus subtilis microbial agent inoculation, inoculum size is taking microbial inoculum service requirements as fixed, and routine is built heap heap fermentation, 2m × 1.5m × 10m.Within three days, turn over once heap, moisturizing in right amount in turning process.About 10 days, fermentation completes.While fermentation, present brownly, free from extraneous odour, without going mouldy.
4, fine day is spread fermentation heap to be exposed to the sun two days out, smashes or pulverizer pulverizing.Composite with reference to ratio below: fermentation wood chip matter bacterium chaff: garden soil: river sand: 70%, 15%, 15%, make lavandula angustifolia wood chip matter bacterium chaff nutrition cultivation matrix.
5, conventional lavandula angustifolia cultivating and growing management.
Foregoing description only proposes as the enforceable technical scheme of the present invention, not as the Single restriction condition to its technical scheme itself.
Claims (8)
1. for cultivating a wood chip bacterium chaff nutrition agent for lavandula angustifolia, it is characterized in that: described wood chip bacterium chaff nutrition agent is on the mixture of wood chip bacterium chaff, fresh mushroom stem lixiviate water, wheat bran and sucrose, inoculate subtilis and make through fermentation; In the batching of described wood chip bacterium chaff nutrition agent, also comprise garden soil and/or husky.
2. prepare claimed in claim 1ly for cultivating the method for wood chip bacterium chaff nutrition agent for lavandula angustifolia, its characterization step comprises:
The preparation of A, the solid-state heap fermentation substratum of wood chip bacterium chaff: get by weight 2 parts of 93 parts of wood chip bacterium chaffs, 5 parts, wheat bran, sucrose and carry out compositely, use fresh mushroom stem lixiviate water spice, material-water ratio is 1:(1.2-1.3);
B, heap fermentation: on the substratum of steps A preparation, carry out bacillus subtilis microbial agent inoculation, build heap heap fermentation, timing turning, has fermented for 8-10 days; Gained zymophyte chaff product comprises the required various nutrients of lavandula angustifolia plant culture;
C, first step B gained fermentation wood chip matter bacterium chaff is adjusted to pH to 7 and then carried out further composite to fermentation wood chip matter bacterium chaff, getting by weight 15 parts, husky 15 parts of 70 parts of fermentation wood chip matter bacterium chaffs, garden soil carries out composite, obtain wood chip bacterium chaff nutrition agent, for the cultivation of lavandula angustifolia.
3. preparation method according to claim 2, is characterized in that: in steps A, first the wood chip bacterium chaff using dries and be crushed to 0.4-0.6cm and sieve, then is used for carrying out the preparation of substratum.
4. preparation method according to claim 2, is characterized in that: in steps A, the preparation method of fresh mushroom stem lixiviate water is: 0.5%NaCl aqueous solution 1:10 proportioning by weight for the waste fresh mushroom stem of pruning, smash micro-boiling 10 minutes, 2 times, filter, merge to obtain filtrate spice.
5. preparation method according to claim 2, is characterized in that: in steps A, use fresh mushroom stem lixiviate water spice, fresh mushroom stem lixiviate water consumption is controlled at the windrow hand seam water outlet degree of holding.
6. preparation method according to claim 2, is characterized in that: while building heap heap fermentation in step B, building heap specification is 2m × 1.5m × 10m; Within in fermenting process three days, turn over once heap.
7. preparation method according to claim 2, is characterized in that: in step C, the zymophyte chaff using first spread out be exposed to the sun 1-3 days and smash after be used for again carrying out composite.
8. preparation method according to claim 2, is characterized in that: in step C, control gained wood chip bacterium chaff nutrition agent and have that draining is good, dry, neutral meta-alkali, the fertile cultivation feature of nutrient.
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| CN108718591B (en) * | 2018-04-28 | 2021-02-09 | 金华市胤宏农业科技有限公司 | Seedling growing method for lavender |
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| CN101759494B (en) * | 2009-10-21 | 2010-12-15 | 山东光大肥业科技有限公司 | Diseases-prevention organic fertilizer prepared by edible fungi residue |
| CN102372505A (en) * | 2010-08-13 | 2012-03-14 | 张红霞 | Processing method of germ bran organic fertilizer |
| CN102701869B (en) * | 2012-06-27 | 2014-02-19 | 中国科学院南京土壤研究所 | Crop straw leach liquor for improving fertilizer efficiency of mycorrhizal fungi fertilizer and preparation method of crop straw leach liquor |
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