CN106045679A - Preparation method of organic compound biological fertilizer special for peanuts - Google Patents
Preparation method of organic compound biological fertilizer special for peanuts Download PDFInfo
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Abstract
The invention discloses a preparation method of an organic compound biological fertilizer special for peanuts. The method comprises the steps that crushed straw and cow dung serve as solid organic materials, 5%. of organic material decomposing agent with a specific decomposing effect and an appropriate amount of water are added and mixed with the organic materials, the water content is maintained to be about 60%, fermentation is conducted for 5 days to 10 days at natural outdoor temperature, and an initial fertilizer is formed through decomposing; an appropriate proportion of various nutrient elements are added and mixed with the fermented materials to be uniform, 4%. of growth promoting compound microbial inoculant special for the peanuts is added, mixing is conducted to be uniform, and the biological fertilizer is obtained. According to the preparation method of the organic compound biological fertilizer special for the peanuts, various disadvantages caused by pure application of chemical fertilizers, ordinary organic fertilizers, farmyard manure and pure microbial fertilizer are overcome, the growth features and demands on nutrients of the peanuts in all periods are fully combined, attention is paid to adjusting of the contradiction between nutrition growth and reproductive growth of the peanuts, and the organic compound biological fertilizer is suitable for peanut growth, efficient and excellent in quality and has a great application prospect in the aspect of peanut production and planting.
Description
Technical field
The present invention relates to a kind of organic composite fertilizer and preparation method thereof, be particularly suited for Semen arachidis hypogaeae, belong to agro-ecology
Technical field prepared by fertilizer.
Background technology
China's chemical fertilizer year usage amount is at about 100,013,000 tons at present, and vast farmers is the most all to use chemical fertilizer,
But the drawback that life-time service chemical fertilizer produces is thereupon, the hardening soil, chemical element residual, environmental pollution etc. are a series of asks
Topic emerges.Chemical fertilizer can not meet the needs of sustainable development, more can not become the pillar of green agriculture, and each state all exists
Find countermeasure.Biological organic fertilizer is born with regard to this, becomes the second revolution of chemical fertilizer.Biological organic fertilizer refers to the micro-life of specific function
Thing with mainly with plant and animal residues (such as feces of livestock and poultry, agricultural crop straw etc.) for originate and through harmless treatment, become thoroughly decomposed organic
The class that material is composited has microbial manure and the fertilizer of fertilizer effect concurrently.
And Semen arachidis hypogaeae is planted in massif gravel soil, Plain alluvialsand and southern red yellow earth mostly, the inclined sand of these soil textures,
Result is bad, the fertility relatively end, uses merely chemical fertilizer and can not meet the nutritional need of Semen arachidis hypogaeae, should enrich biological organic fungi-manure training
Land reform soil, could obtain good quality and high output.Semen arachidis hypogaeae is legume, and it has root nodule, peanut rhizobium to have the strongest nitrogen fixing capacity.
Experiment shows, Semen arachidis hypogaeae to be focused on regulating nitrogen, phosphorus, the suitable proportion of potassium in growth course, and the South and the North exists certain difference.
Additionally, Semen arachidis hypogaeae is happiness calcium crop, the biggest to the absorbtivity of calcium.
Summary of the invention
The special organic composite fertilizer of Semen arachidis hypogaeae of the present invention refers to utilize microbial technique, apolegamy to have specific corruption
The strain of ripe function makes microbial inoculum, is fermented into by SOLID ORGANIC material the most fertile with this microbial inoculum, then adds a large amount of in suitable ratio
Element, trace element etc., then it is equipped with the fertilizer made by Semen arachidis hypogaeae special growth promotion composite bacteria agent capable.Product of the present invention takes full advantage of
There is the characteristic of the function strain that becomes thoroughly decomposed, all kinds of discarded SOLID ORGANIC material fermentation maturities are become fertilizer, turns waste into wealth, save money
Source, greatly reduces environmental pollution；Adding again the growth promotion microbial inoculum that Semen arachidis hypogaeae is special, this growth promotion composite bacteria agent capable is a large amount of through consulting
Data, carry out research summary, according to actual requirement select several beneficial microbes having special efficacy for Semen arachidis hypogaeae, then lead to
Cross reality repeatedly to test, finally determine the optimal proportion of each microorganism, it is ensured that the nutritional need in Semen arachidis hypogaeae each stage.By each strain
Between interaction, play stimulation plant growing, alleviate the hardening soil, improvement ecological environment of soil, disease resistance etc. effect,
Thus reach volume increase, the purpose of high yield.The present invention solves and uses merely chemical fertilizer, common fertilizer, farm manure and pure micro-life
All drawbacks existing for thing bacterial manure.
Decomposing microbial inoculum selected by product of the present invention, it uses bacillus subtilis (Bacillus subtilis)
CICC10088, aspergillus oryzae (Aspergillus niger) CICC2436, whiterot fungi (Phanerochaete
Chrysosporium) ACCC30414, trichoderma reesei (Trichoderma reesei) ACCC30449, stearothermophilus ground spore
Bacillus (Geobacillus stearothermophilus) CICC10207 etc. has the strain of special effect of becoming thoroughly decomposed, by suitably
The microbial bacterial agent of ratio composite fermentation.Selected each strain all can produce cellulase, lignoenzyme, half fiber
The multiple enzyme such as element enzyme, waxiness element enzyme, pectase, by mesophilic bacteria and the synergism of high temperature resistant bacterium, quickly by all kinds of useless
The organic materials fermentation maturity abandoned becomes fertilizer, turns waste into wealth, and saves resource, greatly reduces environmental pollution.
The growth promotion composite bacteria agent capable that Semen arachidis hypogaeae selected by the present invention is special, its contain substantial amounts of Development of Peanut is had special
The beneficial microbe of effect, it uses bacillus subtilis (Bacillus subtilis) ACCC 01183, Bacillus pumilus
(Bacillus pumilus) CGMCC1.937, bacillus megaterium (Bacillus megaterium) ACCC 10010, lichens
Bacillus cereus (Bacillus licheniformis) CICC10037, colloid bacillus cereus (Bacillus
Mucilaginosus) as mix bacterium agent after mixing such as CGMCC 1.153 strain fermentating liquid such as grade, through again ferment and prepare micro-
Biological compound fungi agent.It is through consulting substantial amounts of documents and materials that the present invention chooses each strain, carries out research summary, according to actual requirement
Select several beneficial microbe having special efficacy for Development of Peanut, then repeatedly tested by reality, finally determine
The optimal proportion of each microorganism, thus ensure the nutritional need in Semen arachidis hypogaeae each stage.
The preparation method of the organic composite fertilizer that concrete Semen arachidis hypogaeae of the present invention is special, the method includes walking as follows
Rapid:
(1) preparation of organic matter decomposing inoculant
Strain quantity is respectively reached 108The bacillus subtilis (Bacillus subtilis) of individual/more than mL
CICC10088, aspergillus oryzae (Aspergillus niger) CICC2436, whiterot fungi (Phanerochaete
Chrysosporium) ACCC30414, trichoderma reesei (Trichoderma reesei) ACCC30449, stearothermophilus ground spore
The strain fermentating liquid of bacillus (Geobacillus stearothermophilus) CICC10207 is by 1.5～2.0:0.8～1.3:
After the ratio mix homogeneously of 1.5～2.0:0.8～1.3:0.8～1.3, it is inoculated in the fermentation tank filling composite bacteria agent capable culture medium A
In, inoculum concentration is the 5% of composite bacteria agent capable culture medium A cumulative volume, cultivates 4～7 days, make total bacteria count in fermentation liquid reach at 30 DEG C
108Individual/mL, i.e. obtains organic matter decomposing inoculant；
Described composite bacteria agent capable culture medium A formula is: Semen Maydis flour 0.5～1.5g/L, corn stalk powder 15～17g/L, wheat bran
0.2～0.4g/L, soybean cake powder 0.2～0.4g/L, Vogel ' s mother solution 4mL/100mL, pH value 6.0～8.0；
The concrete preparation process of step () is:
1. actication of culture: described each strain is connected on slant medium；Bacillus subtilis 28～32 DEG C, pH is 7.2
～7.4, incubation time is 1～3 day；Aspergillus oryzae cultivation temperature 25～28 DEG C, pH is 6.0～6.5, and incubation time is 4～7 days；
Whiterot fungi cultivation temperature 25～28 DEG C, incubation time is 4～7 days；Geobacillus stearothermophilus cultivation temperature 55～60 DEG C,
PH7.0-7.2, cultivates 2-3 days；
2. first class inoculum is cultivated: be inoculated in respectively by described strain in the 50mL conical flask filling fluid medium, cultivates
Base pH is 6.0～8.0, and conical flask puts into shaking table shaken cultivation, and temperature is 30 DEG C, vibration 24～48h；Use blood counting chamber meter
Number, in bacterium solution, strain quantity respectively reaches 108Individual/mL；
3. second order fermentation: described strain is inoculated in respectively in the 1000mL conical flask filling fluid medium, culture medium
PH6.0～8.0, puts into shaking table vibration, and temperature is 30 DEG C, vibration 48～72h；Thus obtain strain quantity and respectively reach 108Individual/
Bacillus subtilis (Bacillus subtilis) CICC10088 of more than mL, aspergillus oryzae (Aspergillus niger)
CICC2436, whiterot fungi (Phanerochaete chrysosporium) ACCC30414, trichoderma reesei (Trichoderma
Reesei) ACCC30449, Geobacillus stearothermophilus (Geobacillus stearothermophilus) CICC10207.
Described slant culture based formulas is:
1. the slant culture based formulas that bacillus subtilis uses is: beef infusion broth 1.0L, peptone 10g, NaCl5.0g,
Agar 20.0-20.5g, pH7.2-7.4, the preparation of beef infusion broth: lean beef cleans, chopping, weigh 550g and be soaked in 1375mL
In water, soaking a night, filter, after filtering subpackage, 0.6kgf/cm2 40min is standby.
2. the slant culture based formulas that aspergillus oryzae uses is: 5 0Be wort agars, 5 0Be beerwort 1.0L, agar
15.0g, natural pH.
3. the slant culture based formulas of whiterot fungi is: PDA culture medium, removes the Rhizoma Solani tuber osi 200 grams of skin, is cut into small pieces, adds
1000 milliliters of water boils 30 minutes and filters off potato ball, filtrate complements to 1000 milliliters, adds glucose 20 grams, 15 grams of agar,
Dissolve rear subpackage, 15 pounds of sterilizings 30 minutes；Natural pH.
4. the slant culture based formulas of trichoderma reesei: same to whiterot fungi.
Described fluid medium is that slant medium is not added with agar.
5. Geobacillus stearothermophilus slant culture based formulas: nutrient agar: peptone 10.0g, beef leaches
Thing 3.0g, NaCl5.0g, agar 15.0g, distilled water 1.0L, pH7.0.
(2) puddle, ferment
Organic matter decomposing inoculant step () prepared is mixed in 5 ‰ ratios of fermented organic material gross mass, then
It is blended into appropriate water, makes total moisture control about 60%, at fermentation vat through fermentation in 5-10 days, i.e. prepare the most fertile；
Described fermentation particularly as follows: put fermentation installation into by the fermented organic material of mixing and stirring, and venting is filled up in compacting
Air, fermentation vat top covers layer of plastic film, upper cover sealing and fermenting, turns over buttress in good time, keeps the skin wet, by total moisture during turning
Controlling about 60%, raw material is held agglomerating, and webs have water but do not drip, and looses one's grip and i.e. dissipate preferably.
In preferred technical scheme, the fermentation time of step (two) refers to: general 4-5 days summer, 6-7 days spring and autumn, winter
10 days seasons.
(3) deep processing
Carry out first fertilizer pulverizing, drying, by the most fertile mass percent, the specifically following nutrient of interpolation:
Nitrogen 3.0-5.0%, phosphorus 2.5-3.5%, potassium 1.0-3.0%；Calcium 5.0-10.0%；Humic acid fertilizer 5.0～
10.0%；
Under preferable case, the ratio adding nutrient is:
A. the adding proportion of a great number of elements (nitrogen, phosphorus, potassium): nitrogen 4.0%, phosphorus 3.2%, potassium 2.0%
B. the adding proportion of moderate-element (calcium): calcium 6.0%.
C. the adding proportion of humic acid: 10.0%.
(4) preparation of Semen arachidis hypogaeae special growth promotion composite bacteria agent capable
Strain quantity is respectively reached 108Bacillus subtilis (Bacillus subtilis) ACCC of individual/more than mL
01183, Bacillus pumilus (Bacillus pumilus) CGMCC1.937, bacillus megaterium (Bacillus
Megaterium) ACCC10010, Bacillus licheniformis (Bacillus licheniformis) CICC10037, colloid spore bar
The strain fermentating liquid of bacterium (Bacillus mucilaginosus) CGMCC 1.153, by (20～30%): (10～20%): (10
～20%): (5～10%): as mix bacterium agent after the ratio mix homogeneously of (30～40%), it is inoculated in and fills composite bacteria agent capable training
Supporting in the fermentation tank of base B, inoculum concentration is the 5% of composite bacteria agent capable culture medium B cumulative volume, the pH 6.5 of composite bacteria agent capable culture medium B～
Cultivate 4～7 days at 7.5,30 DEG C, make total bacteria count in fermentation liquid reach 6～10 × 108Individual/mL, i.e. obtains Semen arachidis hypogaeae special microorganism
Composite bacteria agent capable, pH is 6.8, and total living bacteria count is 9 × 108Individual/mL；
The formula of described composite bacteria agent capable culture medium B is: Semen Maydis powder 15～18g/100mL, Semen Glycines powder 5～8g/100mL, ferment
Female cream 0.1～0.2g/100mL, KH2PO40.2～2g/100mL, K2HPO40.5～2g/100mL, MgSO4·7H2O 0.001
～0.02g/100mL, NaCl 0.5～0.8g/100mL, (NH4)2SO40.001～0.01g/100mL, remaining is distilled water；
The detailed process of described step (four) is as follows:
1. inoculation: by described each strain (colloid bacillus cereus, Bacillus pumilus, Bacillus licheniformis, huge spore bar
Bacterium, bacillus subtilis) it is connected to respectively on slant medium；Colloid bacillus cereus (B. mucilaginocus) cultivation temperature 25～35 DEG C, pH
Being 7.5～8.0, incubation time is 5 days；Bacillus pumilus cultivation temperature is 25～30 DEG C, pH7.0-7.2, and incubation time is 3
My god；Bacillus licheniformis cultivation temperature 25～30 DEG C, pH is 7.0～8.0, and incubation time is 5 days；Bacillus megaterium be 27～
37 DEG C, pH is 6.5～7.5, and incubation time is 4 days；Bacillus subtilis cultivation temperature 27～37 DEG C, pH is 6.5～7.5, training
The foster time is 4 days；
2. first class inoculum is cultivated: the strain described step 1 prepared is inoculated in by the amount of inoculating loop one ring respectively and fills phase
Answering in the 50mL conical flask of fluid medium, medium pH is 6.5～7.5, and conical flask puts into shaking table vibration, and temperature is 30 DEG C,
Vibration 24～48h；Employing blood counting chamber counts, and in bacterium solution, strain quantity respectively reaches 108Individual/mL；
3. second order fermentation: the strain described step 2 prepared is inoculated in the 1000mL filling fluid medium respectively by 5%
In conical flask, medium pH 6.5～7.5, put into shaking table vibration, temperature is 30 DEG C, vibration 48～72h；Make strain quantity respectively
Reach 108Individual/more than mL, thus prepare strain quantity and respectively reach 108Bacillus subtilis (the Bacillus of individual/more than mL
Subtilis) ACCC 01183, Bacillus pumilus (Bacillus pumilus) CGMCC1.937, bacillus megaterium
(Bacillus megaterium) ACCC 10010, Bacillus licheniformis (Bacillus licheniformis)
CICC10037, the strain fermentating liquid of colloid bacillus cereus (Bacillus mucilaginosus) CGMCC 1.153.
The slant culture based formulas used in described Semen arachidis hypogaeae complex microbial inoculum preparation process is:
1. the slant culture based formulas that colloid bacillus cereus uses is: KH2PO4 0.06g、MgSO4·7H2O 0.05g、
CaSO4·5H2O 0.004g, peptone 5g, Semen Maydis pulp (50% dry weight) 2.5g, lactose 15g, NaCl 4g, distillation
Water 1000mL, agar 25g, pH 7.1；
2. the slant culture based formulas that Bacillus licheniformis uses is:
Peptone 10.0g, beef leaching thing 3.0g, sodium chloride 5.0g, agar 15g, distilled water 1000mL, pH 7.0；
3. the slant culture based formulas that bacillus megaterium uses is: Carnis Bovis seu Bubali cream 3g, peptone 5g, agar 15g, NaCl
5g, distilled water 1000mL, pH 7.0；
4. the slant culture based formulas that bacillus subtilis uses is: Carnis Bovis seu Bubali cream 10g, peptone 10g, NaCl 5g, Fructus Vitis viniferae
Sugar 10g, agar 15g, distilled water 1000mL, pH 7.0；
5. the slant culture based formulas that Bacillus pumilus uses is: peptone 10.0g, beef leaching thing 3.0g,
NaCl5.0g, agar 15.0g, distilled water 1.0L, pH7.0.
Described liquid culture based formulas be slant medium be not added with agar prepare.
(5) 3～5 ‰ ratios interpolation step (four) of the fermentation materials gross mass obtained in step (three) deep processing obtain
The special growth promotion composite bacteria agent capable of Semen arachidis hypogaeae obtain finished product, after material mix homogeneously, through granulator granulation or become powder, inspection
Survey qualified after, packaging warehouse-in.
For technique scheme, in situations where it is preferred, step (one) described composite bacteria agent capable culture medium A formula is: beautiful
Rice and flour 0.5g/L, corn stalk powder 15g/L, wheat bran 0.25g/L, soybean cake powder 0.25g/L, Vogel ' s mother solution 4mL/100mL, pH
8.5。
For technique scheme, in situations where it is preferred, step (one) described bacillus subtilis (Bacillus
Subtilis) CICC10088, aspergillus oryzae (Aspergillus niger) CICC2436, whiterot fungi (Phanerochaete
Chrysosporium) ACCC30414, trichoderma reesei (Trichoderma reesei) ACCC30449, stearothermophilus ground spore
The strain fermentating liquid of bacillus (Geobacillus stearothermophilus) CICC10207 is mixed in the ratio of 2:1:2:1:1
Close uniformly.
For technique scheme, in situations where it is preferred, step (two) described fermented organic material is the Semen Maydis pulverized
Straw is mixed by 0.5～1.5:0.2～1.0 mass ratioes with animal wastes, and described animal wastes are usually chicken manure or cattle manure.
For technique scheme, in situations where it is preferred, the formula of step (three) described composite bacteria agent capable culture medium B is:
Semen Maydis powder 16g/100mL, Semen Glycines powder 7g/100mL, yeast extract 0.1g/100mL, KH2PO4 1g/100mL、K2HPO4 1g/
100mL、MgSO4·7H2O 0.01g/100mL、NaCl 0.6g/100mL、(NH4)2SO40.01g/100mL, remaining is distillation
Water.
The technical scheme is that the unremitting effort through in recent years, in conjunction with the growth characteristic in Semen arachidis hypogaeae each period and right
The demand of nutrient, notes regulating its contradiction with reproductive growth of nourishing and growing, and the biology finally developing a kind of high-efficiency high-quality has
Machine is fertile.Its integrated use newest fruits such as microbial technique, chemical fertilizer industry and agricultural technology, are preferentially prepared for a kind of efficient
Combine rich in organic, N P K nutrient and some material such as trace element, plant growth regulator, by
Special process has ultimately formed the graininess nutrient source that the nutrition based on organic matter is reasonable, N P K ratio is suitable.This kind
Fertilizer not only has high usage, the advantage of fertilizer efficiency period length, and has improvement soil, improves crops quality, strengthens crop
Resistance, increases yield, reduces and pollutes, and stimulates the feature such as crop growth, time and labour saving, have good social benefit and
Economic benefit.
Beneficial effect
The special biological organic fertilizer of Semen arachidis hypogaeae of the present invention is to select to have the specific strain with function of becoming thoroughly decomposed and make
Microbial inoculum, becomes thoroughly decomposed into the most fertile with this microbial inoculum by organic solid material fermentation, then by suitable ratio interpolation a great number of elements (nitrogen, phosphorus,
Potassium), moderate-element (calcium), humic acid etc., then be equipped with effect and promote the fertilizer made by microbial inoculum of peanut growth accordingly.Become thoroughly decomposed
Containing substantial amounts of bacillus subtilis, aspergillus oryzae, trichoderma reesei, whiterot fungi, Geobacillus stearothermophilus in agent, it is possible to produce
The multiple enzyme such as cellulase, lignoenzyme, hemicellulase, waxiness element enzyme, pectase, by mesophilic bacteria and high temperature resistant bacterium
Synergism, quickly all kinds of discarded SOLID ORGANIC material fermentation maturities are become fertilizer, turn waste into wealth, save resource, significantly
Decrease environmental pollution；Add again all kinds of nutrient and the special growth promotion composite bacteria agent capable of Semen arachidis hypogaeae, special by Semen arachidis hypogaeae is had
The beneficial microbe bacillus subtilis of effect, Bacillus licheniformis, bacillus megaterium, Candida utilis, soybean nodulation
Interaction between bacterium, colloid bacillus cereus, each strain of Lactobacillus plantarum, play stimulation plant growing, alleviate the hardening soil,
Improvement ecological environment of soil, the humus increasing soil and the effect such as beneficial microbe colony, disease resistance, thus reach to increase
Product, the purpose of high yield.
Detailed description of the invention
The following non-limiting example of the present invention can make those of ordinary skill in the art that this is more fully understood
Bright, but limit the present invention never in any form.Biomaterial used in embodiment all can be by being either commercially available.
Embodiment one: the preparation of the special organic composite fertilizer of Semen arachidis hypogaeae
The special organic composite fertilizer product of Semen arachidis hypogaeae of the present invention refers to utilize microbial technique, apolegamy to have specific becoming thoroughly decomposed
The strain of function makes microbial inoculum, organic solid material fermentation is become thoroughly decomposed into the most fertile with this microbial inoculum, then be equipped with various nutrient and
The bio-feritlizer made by growth promotion composite bacteria agent capable that Semen arachidis hypogaeae is special.Its detailed description of the invention is as follows:
(1) preparation of organic matter decomposing inoculant
Use bacillus subtilis (Bacillus subtilis) CICC10088, aspergillus oryzae (Aspergillus
Niger) CICC2436, whiterot fungi (Phanerochaete chrysosporium) ACCC30414, trichoderma reesei
(Trichoderma reesei) ACCC30449, Geobacillus stearothermophilus (Geobacillus
Stearothermophilus) CICC10207 etc. have efficiently become thoroughly decomposed effect strain composite fermentation in appropriate proportion form
Decomposing microbial inoculum.
1. actication of culture: described each strain is connected on slant medium；Bacillus subtilis 28～32 DEG C, pH is 7.2
～7.4, incubation time is 1～3 day；Aspergillus oryzae cultivation temperature 25～28 DEG C, pH is 6.0～6.5, and incubation time is 4～7 days；
Whiterot fungi cultivation temperature 25～28 DEG C, incubation time is 4～7 days；Geobacillus stearothermophilus cultivation temperature 55～60 DEG C,
PH7.0-7.2, cultivates 2-3 days；
2. first class inoculum is cultivated: be inoculated in respectively by described strain in the 50mL conical flask filling fluid medium, cultivates
Base pH is 6.0～8.0, and conical flask puts into shaking table shaken cultivation, and temperature is 30 DEG C, vibration 24～48h；Use blood counting chamber meter
Number, in bacterium solution, strain quantity respectively reaches 108Individual/mL；
3. second order fermentation: described strain is inoculated in respectively in the 1000mL conical flask filling fluid medium, culture medium
PH6.0～8.0, puts into shaking table vibration, and temperature is 30 DEG C, vibration 48～72h；Strain quantity is made to respectively reach 108Individual/mL with
On, by each strain by the mass percent mixing of described strain；
4. three grade fermemtation: respectively by bacillus subtilis (Bacillus subtilis) CICC10088, aspergillus oryzae
(Aspergillus niger) CICC2436, whiterot fungi (Phanerochaete chrysosporium) ACCC30414, Richter scale
Trichoderma spp. (Trichoderma reesei) ACCC30449, Geobacillus stearothermophilus (Geobacillus
Stearothermophilus), after the strain fermentating liquid of CICC10207 is in the ratio mix homogeneously of 2:1:2:1:1, it is inoculated in Sheng
Having in the fermentation tank of composite bacteria agent capable culture medium, composite bacteria agent capable medium pH 6.0～8.0, inoculum concentration is 5%, cultivates 4 at 30 DEG C
～7 days, make bacterium number in fermentation liquid reach 108Individual/mL, i.e. obtains organic materials straw decomposing inoculant.
Described slant culture based formulas is:
1. the slant culture based formulas that bacillus subtilis uses is: plain agar: beef infusion broth 1.0L, peptone 10g,
NaCl5.0g, agar 20.0-20.5g, pH7.2-7.4, the preparation of beef infusion broth: lean beef cleans, chopping, weigh 550g and soak
In 1375mL water, soak a night, filter, filter 0.6kgf/cm after subpackage240min is standby.
2. the slant culture based formulas that aspergillus oryzae uses is: 5 ° of Be wort agars, 5 ° of Be beerwort 1.0L, agar
15.0g, natural pH.
3. the slant culture based formulas of whiterot fungi is: PDA culture medium, removes the Rhizoma Solani tuber osi 200 grams of skin, is cut into small pieces, adds
1000 milliliters of water boils 30 minutes and filters off potato ball, filtrate complements to 1000 milliliters, adds glucose 20 grams, 15 grams of agar,
Dissolve rear subpackage, 15 pounds of sterilizings 30 minutes.Natural pH.
4. the slant culture based formulas of trichoderma reesei is: with whiterot fungi formula.
5. Geobacillus stearothermophilus slant culture based formulas: nutrient agar: peptone 10.0g, beef leaches
Thing 3.0g, NaCl5.0g, agar 15.0g, distilled water 1.0L, pH7.0.
Described fluid medium is that slant medium is not added with agar.
Described composite bacteria agent capable culture medium prescription is: Semen Maydis flour 0.5g/L, corn stalk powder 15g/L, wheat bran 0.25g/L, bean
Cake powder 0.25g/L, Vogel ' s mother solution 4mL/100mL, pH value 8.5.
(2) puddle, ferment
The corn straw that preparation 500kg pulverizes and 200kg cattle manure are as the initial organic matter material fermented, by having of preparing
Machine material decomposing agent is blended in appropriate water by the 5 ‰ of fermented organic material gross mass, uniformly puddles with organic materials, raw material hands
Holding agglomerating, webs have water but do not drip, and loose one's grip and i.e. dissipate (total moisture about 60%) preferably.The fermentation of mixing and stirring is had
Fermentation installation put into by machine material, and venting air is filled up in compacting, and fermentation vat top covers layer of plastic film, upper cover sealing and fermenting,
Turn over buttress in good time, keep the skin wet during turning, total moisture is controlled about 60%.Through fermentation in 10 days, i.e. prepare the most fertile.
(3) interpolation of all kinds of nutrients
After material fermentation completes, carry out pulverizing, drying by fertile dosage form requirement, then add all kinds of nutrient, including big
Secondary element (nitrogen, phosphorus, potassium), moderate-element (calcium), by fermentation materials Mass Calculation, concrete adding proportion is as follows:
A. the adding proportion of a great number of elements (nitrogen, phosphorus, potassium): nitrogen 4.0%, phosphorus 3.2%, potassium 2.0%.
B. the adding proportion of moderate-element (calcium): calcium 6.0%.
C. the adding proportion of ammonium humate: 10%.
(4) preparation of Semen arachidis hypogaeae special growth promotion composite bacteria agent capable and interpolation
Use bacillus subtilis (Bacillus subtilis) ACCC 01183, Bacillus pumilus (Bacillus
Pumilus) CGMCC1.937, bacillus megaterium (Bacillus megaterium) ACCC 10010, Bacillus licheniformis
(Bacillus licheniformis) CICC10037, colloid bacillus cereus (Bacillus mucilaginosus) CGMCC
After the strain fermentating liquid mixing with special efficacy such as 1.153 multiple as mix bacterium agent, the microorganism prepared through again fermenting
Close microbial inoculum.
Described Semen arachidis hypogaeae complex microbial inoculum preparation process is as follows:
1. inoculation: by described each strain (colloid bacillus cereus, Bacillus pumilus, Bacillus licheniformis, huge spore bar
Bacterium, bacillus subtilis) it is connected to respectively on slant medium；Colloid bacillus cereus (B. mucilaginocus) cultivation temperature 25～35 DEG C, pH
Being 7.5～8.0, incubation time is 5 days；Bacillus pumilus cultivation temperature is 25～30 DEG C, pH7.0-7.2, and incubation time is 3
My god；Bacillus licheniformis cultivation temperature 25～30 DEG C, pH is 7.0～8.0, and incubation time is 5 days；Bacillus megaterium be 27～
37 DEG C, pH is 6.5～7.5, and incubation time is 4 days；Bacillus subtilis cultivation temperature 27～37 DEG C, pH is 6.5～7.5, training
The foster time is 4 days；
2. first class inoculum is cultivated: the strain described step 1 prepared is inoculated in by the amount of inoculating loop one ring respectively and fills phase
Answering in the 50mL conical flask of fluid medium, medium pH is 6.5～7.5, and conical flask puts into shaking table vibration, and temperature is 30 DEG C,
Vibration 24～48h；Employing blood counting chamber counts, and in bacterium solution, strain quantity respectively reaches 108Individual/mL；
3. second order fermentation: the strain described step 2 prepared is inoculated in the 1000mL filling fluid medium respectively by 5%
In conical flask, medium pH 6.5～7.5, put into shaking table vibration, temperature is 30 DEG C, vibration 48～72h；Make strain quantity respectively
Reach 108Individual/more than mL, thus prepare colloid bacillus cereus (B. mucilaginocus) fermentation liquid, aztobacter sp fermentation liquid, huge
Fermentation of bacillus liquid and fermentation of bacillus subtilis liquid.
Again ferment after each strain is mixed by mass percentage: colloid bacillus cereus (B. mucilaginocus) fermentation liquid
35%, bacillus megaterium fermentation liquid 25%, the lichen bacillus ferments liquid 10%, fermentation of bacillus subtilis liquid 30%.
4. three grade fermemtation: the mixed bacterium solution described step 3 prepared is inoculated in and fills sending out of composite bacteria agent capable culture medium
In ferment tank, composite bacteria agent capable medium pH 6.5～7.5, inoculum concentration is the 5% of composite bacteria agent capable culture medium gross mass, cultivates at 30 DEG C
4～7 days, bacterium number in fermentation liquid is made to reach 6～10 × 108Individual/mL, i.e. obtains Semen arachidis hypogaeae special microorganism composite bacterial agent, and pH is
5.0, bacterium number content all reaches 109Individual/mL.
Described slant culture based formulas is:
1. the slant culture based formulas that colloid bacillus cereus uses is: KH2PO4 0.06g、MgSO4·7H2O 0.05g、
CaSO4·5H2O 0.004g, peptone 5g, Semen Maydis pulp (50% dry weight) 2.5g, lactose 15g, NaCl 4g, distillation
Water 1000mL, agar 25g, pH 7.1；
2. the slant culture based formulas that Bacillus licheniformis uses is: peptone 10.0g, beef leaching thing 3.0g, chlorination
Sodium 5.0g, agar 15g, distilled water 1000mL, pH 7.0；
3. the slant culture based formulas that bacillus megaterium uses is: Carnis Bovis seu Bubali cream 3g, peptone 5g, agar 15g, NaCl
5g, distilled water 1000mL, pH 7.0；
4. the slant culture based formulas that bacillus subtilis uses is: Carnis Bovis seu Bubali cream 10g, peptone 10g, NaCl 5g, Fructus Vitis viniferae
Sugar 10g, agar 15g, distilled water 1000mL, pH 7.0；
5. the slant culture based formulas that Bacillus pumilus uses is: peptone 10.0g, beef leaching thing 3.0g,
NaCl5.0g, agar 15.0g, distilled water 1.0L, pH7.0.
Described liquid culture based formulas be slant medium be not added with agar prepare.
Described composite bacteria agent capable culture medium prescription is: Semen Maydis powder 2～20g/100mL, Semen Glycines powder 1～10g/100mL, yeast extract
0.05～0.2g/100mL, KH2PO40.2～2g/100mL, K2HPO40.5～2g/100mL, MgSO4·7H2O 0.001～
0.02g/100mL, NaCl 0.5～0.8g/100mL, (NH4)2SO40.001～0.01g/100mL, remaining is distilled water.
The growth promotion composite bacteria agent capable that Semen arachidis hypogaeae is special, mix homogeneously again is added in 4 ‰ ratios of fermentation materials gross mass.
(5) pelletize or one-tenth powder, detect, pack, put in storage
After material mix homogeneously, through granulator granulation or become powder, detect qualified after, packaging warehouse-in.
Embodiment two: Semen arachidis hypogaeae special organic composite fertilizer application effect on Semen arachidis hypogaeae
The effect test that the special organic composite fertilizer of Semen arachidis hypogaeae using the present invention to prepare is applied in peanut cultivation:
1. test objective
The special organic composite fertilizer of Semen arachidis hypogaeae is the raw for Semen arachidis hypogaeae of the up-to-date development of DaLian Sanke Bio-engineering Co., Ltd.
The long bio-feritlizer having special efficacy, for its impact on Development of Peanut clear and definite, 4-9 month in 2015, my company was for flower
Life has carried out field plot trial.
2. experimental condition: test site is Jinzhou district of Dalian, and plot is representative.Physical features is smooth, middle fertility, front
Stubble crop is Semen arachidis hypogaeae.
3. test material and method
Experimental cultivar: Semen arachidis hypogaeae (flower educates No. 22)
Test method: test is set to two groups, uses random alignment, repeats for three times respectively, each experimental plot area
66.7 square metres.
Two groups of described process:
1. matched group, conventional fertilizer application；2. test group, uses in strict accordance with organic composite fertilizer product operation instruction.
Through actual survey, within 2015, Semen arachidis hypogaeae period of duration natural conditions are preferable, without the life of special vile weather, beneficially Semen arachidis hypogaeae
Long.
4. investigation records method
The record Semen arachidis hypogaeae period of maturation selects 10 strains at each community random continuous, surveys stem height, side shoot length, total branch amount, single
The production targets such as the double full fruit number of strain, BAIGUO number and cell production.
5. result and analysis
The impact on peanut nutrition character of 5.1 different fertilization
After Semen arachidis hypogaeae is emerged, before occurring to the 4th true leaf, each Semen arachidis hypogaeae growing way processed is essentially identical；True leaf occur with
After, the Semen arachidis hypogaeae growing way of test group is significantly better than comparison, until during fruit maturation.Data result is shown in Table 1:
The impact on peanut nutrition character of table 1. different fertilization
As it can be seen from table 1 the every character of test group Semen arachidis hypogaeae is substantially better than matched group, dark green leaf, plant type is good, neither
Excessive growth has again enough effective branches, the side shoot length of process group and effective branch amount 42.3cm and 8.9, more than matched group
6.9cm and 0.9, observe harvest time, and the stem green blade of process group is significantly more than matched group, and this fills for Fruit demand
The photosynthate of foot provides guarantee.
The impact on peanut yield character of 5.2 different fertilization
The impact on peanut yield character of table 2. different fertilization
As can be seen from Table 2, the double full fruit number of process group Semen arachidis hypogaeae individual plant exceeds 3.5 than matched group, and BAIGUO heavily adds
24.7g, kernel percent is high 4.6%, shows that using the special organic composite fertilizer of Semen arachidis hypogaeae can improve the yield and quality of Semen arachidis hypogaeae.
The impact on Semen arachidis hypogaeae fruit output economics character of 5.3 different disposal
The impact on Semen arachidis hypogaeae fruit output economics character of table 3. different fertilization
| Process | Yield (kg/ mu) | Units increased in production (kg/ mu) | Net rate of growth (%) | Have a net increase of product (unit/mu) |
| Matched group | 409 | 83 | 20.2 | 332 |
| Process group | 492 | -- | -- | -- |
Substantially increase from table 3 it can be seen that use Semen arachidis hypogaeae special organic composite fertilizer peanut yield, increase production than matched group
83kg/ mu, rate of growth is 20.2%, calculates by 4 yuan/kg of Semen arachidis hypogaeae price, and every mu has a net increase of receipts 332 yuan, and economic benefit is the most considerable.
6. conclusion
Semen arachidis hypogaeae special organic composite fertilizer application effect on Semen arachidis hypogaeae is notable, in peanut nutrition character, growth traits
And yield traits aspect all shows obvious advantage, substantially increase economic benefit.Whole trophophase growing way is neat, Ye Senong
Green abundant.Therefore, Semen arachidis hypogaeae special organic composite fertilizer test effect on Semen arachidis hypogaeae proves, this product can be in peanut production
Expand demonstration area, and carry out spread use.
Claims (6)
1. one cultivate peanut special organic composite fertilizer, it is characterised in that
(1) preparation of organic matter decomposing inoculant
Strain quantity is respectively reached 108Bacillus subtilis (Bacillus subtilis) CICC10088 of individual/more than mL,
Aspergillus oryzae (Aspergillus niger) CICC2436, whiterot fungi (Phanerochaete chrysosporium)
ACCC30414, trichoderma reesei (Trichoderma reesei) ACCC30449, Geobacillus stearothermophilus
The strain fermentating liquid of (Geobacillus stearothermophilus) CICC10207 presses 1.5～2.0:0.8～1.3:1.5
～after the volume ratio mix homogeneously of 2.0:0.8～1.3:0.8～1.3, it is inoculated in the fermentation tank filling composite bacteria agent capable culture medium A
In, inoculum concentration is the 5% of composite bacteria agent capable culture medium A cumulative volume, cultivates 4～7 days, make total bacteria count in fermentation liquid reach at 30 DEG C
108Individual/mL, i.e. obtains organic matter decomposing inoculant；
Described composite bacteria agent capable culture medium A formula is: Semen Maydis flour 0.5～1.5g/L, corn stalk powder 15～17g/L, wheat bran 0.2～
0.4g/L, soybean cake powder 0.2～0.4g/L, Vogel ' s mother solution 4mL/100mL, pH value 6.0～8.0；
(2) puddle, ferment
Organic matter decomposing inoculant step () prepared is in 5 ‰ ratio mixing of fermented organic material gross mass, then is blended into
Appropriate water, makes total moisture control about 60%, at fermentation vat through fermentation in 5-10 days, i.e. prepares the most fertile；
(3) deep processing
Carry out first fertilizer pulverizing, drying, by the most fertile mass percent, the specifically following nutrient of interpolation:
Nitrogen 3.0-5.0%, phosphorus 2.5-3.5%, potassium 1.0-3.0%；Calcium 5.0-10.0%；Humic acid fertilizer 5.0～10.0%；
(4) preparation of Semen arachidis hypogaeae special growth promotion composite bacteria agent capable
Strain quantity is respectively reached 108Bacillus subtilis (Bacillus subtilis) ACCC 01183 of individual/more than mL,
Bacillus pumilus (Bacillus pumilus) CGMCC1.937, bacillus megaterium (Bacillus megaterium)
ACCC10010, Bacillus licheniformis (Bacillus licheniformis) CICC10037, colloid bacillus cereus (Bacillus
Mucilaginosus) strain fermentating liquid of CGMCC 1.153, by (20～30%): (10～20%): (10～20%): (5～
10%): as mix bacterium agent after the ratio mix homogeneously of (30～40%), be inoculated in the fermentation filling composite bacteria agent capable culture medium B
In tank, inoculum concentration is the 5% of composite bacteria agent capable culture medium B cumulative volume, the pH 6.5～7.5 of composite bacteria agent capable culture medium B, trains at 30 DEG C
Support 4～7 days, make total bacteria count in fermentation liquid reach 6～10 × 108Individual/mL, i.e. obtains Semen arachidis hypogaeae special microorganism composite bacterial agent, pH
Being 6.8, total living bacteria count is 9 × 108Individual/mL；
The formula of described composite bacteria agent capable culture medium B is: Semen Maydis powder 15～18g/100mL, Semen Glycines powder 5～8g/100mL, yeast extract
0.1～0.2g/100mL, KH2PO40.2～2g/100mL, K2HPO40.5～2g/100mL, MgSO4·7H2O 0.001～
0.02g/100mL, NaCl 0.5～0.8g/100mL, (NH4)2SO40.001～0.01g/100mL, remaining is distilled water；
(5) 3～5 ‰ ratios of the fermentation materials gross mass obtained in step (three) deep processing add what step (four) obtained
The growth promotion composite bacteria agent capable acquisition finished product that Semen arachidis hypogaeae is special.
The preparation method of the organic composite fertilizer that Semen arachidis hypogaeae the most according to claim 1 is special, it is characterised in that step
(1) described composite bacteria agent capable culture medium A formula is: Semen Maydis flour 0.5g/L, corn stalk powder 15g/L, wheat bran 0.25g/L, soybean cake powder
0.25g/L, Vogel ' s mother solution 4mL/100mL, pH 7.2.
The preparation method of the organic composite fertilizer that Semen arachidis hypogaeae the most according to claim 1 is special, it is characterised in that step
(1) described bacillus subtilis (Bacillus subtilis) CICC10088, aspergillus oryzae (Aspergillus niger)
CICC2436, whiterot fungi (Phanerochaete chrysosporium) ACCC30414, trichoderma reesei (Trichoderma
Reesei) ACCC30449, Geobacillus stearothermophilus (Geobacillus stearothermophilus) CICC10207
Strain fermentating liquid in the ratio mix homogeneously of 2:1:2:1:1.
The preparation method of the organic composite fertilizer that Semen arachidis hypogaeae the most according to claim 1 is special, it is characterised in that step
(2) described fermented organic material is that the corn straw pulverized is mixed by 0.5～1.5:0.2～1.0 mass ratioes with animal wastes.
The preparation method of the organic composite fertilizer that Semen arachidis hypogaeae the most according to claim 1 is special, it is characterised in that step
(3) described nutrient is nitrogen 4.0%, phosphorus 3.2%, potassium 2.0%；Calcium 6.0%；Humic acid fertilizer 10.0%, humic-acid kind
Fertilizer is ammonium humate or potassium humate.
The preparation method of the organic composite fertilizer that Semen arachidis hypogaeae the most according to claim 1 is special, it is characterised in that step
(3) formula of described composite bacteria agent capable culture medium B is: Semen Maydis powder 16g/100mL, Semen Glycines powder 7g/100mL, yeast extract 0.1g/
100mL、KH2PO4 1g/100mL、K2HPO4 1g/100mL、MgSO4·7H2O 0.01g/100mL、NaCl 0.6g/100mL、
(NH4)2SO40.01g/100mL, remaining is distilled water.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106831249A (en) * | 2017-02-28 | 2017-06-13 | 钱灿星 | A kind of special organic calcareous fertilisers high of Peanut Pod Periods |
| CN108218567A (en) * | 2018-01-04 | 2018-06-29 | 中化农业（临沂）研发中心有限公司 | One cultivates peanut special bio type organic-inorganic compound fertilizer |
| CN110272305A (en) * | 2019-05-05 | 2019-09-24 | 南京农业大学 | A kind of botanical material During High-Temperature Composting method and its used in emptins |
| CN113372146A (en) * | 2021-05-28 | 2021-09-10 | 淮北师范大学 | Biological organic-inorganic ternary compound fertilizer containing plant growth-promoting bacteria and preparation method thereof |
| CN115466690A (en) * | 2022-05-19 | 2022-12-13 | 同济大学 | Novel geobacillus strain as well as culture method and application thereof |
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| CN102199049A (en) * | 2010-12-15 | 2011-09-28 | 沈阳科丰牧业科技有限公司 | Microbial organic manure and preparation method thereof |
| CN104692866A (en) * | 2013-12-06 | 2015-06-10 | 大连三科生物工程有限公司 | Biological organic fertilizer |
| CN104862247A (en) * | 2015-04-30 | 2015-08-26 | 山西师范大学 | Liquid medium of composite microbial agent and preparation method thereof |
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| CN102199049A (en) * | 2010-12-15 | 2011-09-28 | 沈阳科丰牧业科技有限公司 | Microbial organic manure and preparation method thereof |
| CN104692866A (en) * | 2013-12-06 | 2015-06-10 | 大连三科生物工程有限公司 | Biological organic fertilizer |
| CN104862247A (en) * | 2015-04-30 | 2015-08-26 | 山西师范大学 | Liquid medium of composite microbial agent and preparation method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106831249A (en) * | 2017-02-28 | 2017-06-13 | 钱灿星 | A kind of special organic calcareous fertilisers high of Peanut Pod Periods |
| CN108218567A (en) * | 2018-01-04 | 2018-06-29 | 中化农业（临沂）研发中心有限公司 | One cultivates peanut special bio type organic-inorganic compound fertilizer |
| CN110272305A (en) * | 2019-05-05 | 2019-09-24 | 南京农业大学 | A kind of botanical material During High-Temperature Composting method and its used in emptins |
| CN113372146A (en) * | 2021-05-28 | 2021-09-10 | 淮北师范大学 | Biological organic-inorganic ternary compound fertilizer containing plant growth-promoting bacteria and preparation method thereof |
| CN115466690A (en) * | 2022-05-19 | 2022-12-13 | 同济大学 | Novel geobacillus strain as well as culture method and application thereof |
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