CN106045677A - Method for preparing organic compound biological fertilizer special for fruit trees - Google Patents

Method for preparing organic compound biological fertilizer special for fruit trees Download PDF

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Publication number
CN106045677A
CN106045677A CN201610371039.3A CN201610371039A CN106045677A CN 106045677 A CN106045677 A CN 106045677A CN 201610371039 A CN201610371039 A CN 201610371039A CN 106045677 A CN106045677 A CN 106045677A
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fertilizer
fruit tree
special
agent capable
organic
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崔昌娜
崔国臣
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DALIAN SANKE BIO-ENGINEERING Co Ltd
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DALIAN SANKE BIO-ENGINEERING Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00—Fertilisers from human or animal excrements, e.g. manure
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F9/00—Fertilisers from household or town refuse
    • C05F9/04—Biological compost
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention discloses an organic compound biological fertilizer special for fruit trees. Smashed straw and cow dung serve as solid organic materials, and an organic material decomposition agent having a specific thorough-decomposition function at a proportion of 5%. is added and an appropriate amount of water is added; after the mixing with the organic material, the water content is maintained to be about 60%, and fermentation is performed at natural outdoor temperature for 5-10 days to form a primary fertilizer. After various nutrient elements at an appropriate proportion are added to be evenly mixed with the fermented material, a growth-promoting combined fungus at a proportion of 4%. is added, and then a bio-fertilizer is obtained after uniform mixing. Various shortcomings in pure application of fertilizers, ordinary organic fertilizers, farm manure and pure microorganism bacterium fertilizer are overcome, growth characteristics at each stage of the fruit trees and nutrient demand are fully combined, nutrition growth and reproductive growth contradiction is overcome, and the organic compound biological fertilizer is an efficient and high-quality organic compound biological fertilizer particularly suitable for fruit tree growth. The organic compound biological fertilizer has good application prospects in the fruit tree planting aspect.

Description

The preparation method of the organic composite fertilizer that one planting fruit-trees is special
Technical field
The present invention relates to a kind of organic composite fertilizer and preparation method thereof, be particularly suited for fruit tree, belong to agro-ecology Technical field prepared by fertilizer.
Background technology
China's chemical fertilizer year usage amount is at about 100,013,000 tons at present, and vast farmers is the most all to use chemical fertilizer, But the drawback that life-time service chemical fertilizer produces is thereupon, the hardening soil, chemical element residual, environmental pollution etc. are a series of asks Topic emerges.Chemical fertilizer can not meet the needs of sustainable development, more can not become the pillar of green agriculture, and each state all exists Find countermeasure.Biological organic fertilizer is born with regard to this, becomes the second revolution of chemical fertilizer.Material is compound and biological organic fertilizer refers to spy Determine functional microorganism with mainly with plant and animal residues (such as feces of livestock and poultry, agricultural crop straw etc.) for originate and through harmless treatment, One class of the organic one-tenth become thoroughly decomposed has microbial manure and the fertilizer of fertilizer effect concurrently.
And fruit tree refers to fruit, the perennial plant of seed and the general name of stock thereof that offer can be edible.Fruit tree exists Nutritional labeling needed for each period of growth is that there is some difference.The growth early stage substantial amounts of nitrogen of needs, young sprout growth, Star-spangled banner and growth of young are required for substantial amounts of nitrogen.But the nitrogen that this period needs is mainly derived from reserving nutrient of tree body.Cause It is particularly significant that this increases reserving nutrient of nitrogen.After entering late June, nitrogen demand reduces.And before dormancy of gathering, Be root system regrow peak, be also the important storage period of nitrogen, to nitrogen demand rebound significantly again.To P elements Absorbing, show as early growth period and increase sharply, the florescence reaches to absorb peak, maintains higher level the most always.Tree body is to potassium unit The demand of element shows as front low, middle high, rear low, i.e. florescence demand is few, and the later stage is gradually increased, and reaches to August fruit expanding period To peak, the later stage is gradually reduced again.Meanwhile, the nutrient such as calcium, magnesium, boron, zinc is all necessary nutrition during fruit tree growth Element, will carry out suitable supplementing.Therefore, use merely chemical fertilizer and can not meet the nutritional need of fruit tree growth, need to enrich Biological organic fertilizer is that it persistently supplements nutrient.
In sum, biological organic fungi-manure be agricultural development be hopeful most, the most promising irreplaceable product.I am public Department research staff is through unremitting effort in recent years, in conjunction with the growth characteristic in each period of fruit tree, finally develops a kind of being suitable for High-quality biological organic fertilizer in fruit tree.Our company invention product integrated use microbial technique, chemical fertilizer industry and agricultural technology Etc. newest fruits, preferentially it is prepared for a kind of efficiently rich in organic, N P K nutrient and some trace element, plant The materials such as growth regulator combine, to special process ultimately formed the nutrition based on organic matter rationally, N The graininess nutrient source that P K ratio is suitable.This kind of fertilizer not only has high usage, the advantage of fertilizer efficiency period length, and has and change The spies such as good soil, raising crops quality, strengthen crop anti-adversity, increase yield, reduce and pollute, stimulation crop growth Point, time and labour saving, there is good Social benefit and economic benefit.
Summary of the invention
The special biological organic fertilizer of fruit tree of the present invention refers to utilize microbial technique, apolegamy to have specific merit of becoming thoroughly decomposed The strain of energy makes microbial inoculum, is fermented into by SOLID ORGANIC material the most fertile with this microbial inoculum, then adds all kinds of nutrition in suitable ratio Element, then it is equipped with the fertilizer made by fruit tree special growth promotion composite bacteria agent capable.Product of the present invention takes full advantage of has merit of becoming thoroughly decomposed All kinds of discarded SOLID ORGANIC material fermentation maturities are become fertilizer, turn waste into wealth by the characteristic of energy strain, save resource, significantly subtract Lack environmental pollution；Adding again the growth promotion microbial inoculum that fruit tree is special, this growth promotion composite bacteria agent capable is through consulting substantial amounts of data, entering Row research summary, selects several beneficial microbes having special efficacy for fruit tree according to actual requirement, then by actual anti- Multiple experiment, finally determines the optimal proportion of each microorganism, it is ensured that the nutritional need in fruit tree each stage.By the phase between each strain Interaction, plays stimulation plant growing, alleviates the hardening soil, the improvement effect such as ecological environment of soil, disease resistance, thus reach Volume increase, the purpose of high yield.The present invention solves and uses merely chemical fertilizer, common fertilizer, farm manure and pure microbial-bacterial fertilizer institute The all drawbacks existed.
Decomposing microbial inoculum selected by product of the present invention, it uses bacillus subtilis (Bacillus subtilis) CICC10088, aspergillus oryzae (Aspergillus niger) CICC2436, whiterot fungi (Phanerochaete Chrysosporium) ACCC30414, trichoderma reesei (Trichoderma reesei) ACCC30449 etc. have special merit of becoming thoroughly decomposed The strain of effect, the microbial bacterial agent of composite fermentation in appropriate proportion.Selected each strain all can produce cellulase, The multiple enzyme such as lignoenzyme, hemicellulase, waxiness element enzyme, pectase, by mesophilic bacteria and the collaborative work of high temperature resistant bacterium With, quickly all kinds of discarded organic materials fermentation maturities are become fertilizer, turn waste into wealth, save resource, greatly reduce environment dirty Dye.
The growth promotion composite bacteria agent capable that fruit tree selected by the present invention is special, its contain substantial amounts of growth and development of fruit tree is had special The beneficial microbe of effect, it uses streptomyces microflavus (Streptomyces microflavus) CGMCC4.891, colloid bud Spore bacillus (Bacillus mucilaginosus) CGMCC 1.153, aztobacter sp (Azotobacter Chroococcum) CGMCC 1.142, bacillus megaterium (Bacillus megaterium) ACCC 10010, hay spore As mix bacterium agent after mixing such as bacillus (Bacillus subtilis) ACCC 01183 strain fermentating liquid such as grade, through fermentation again The complex microbial inoculum of preparation.It is through consulting substantial amounts of documents and materials that the present invention chooses each strain, carries out research summary, according to Actual requirement selects several beneficial microbes having special efficacy for growth and development of fruit tree, is then repeatedly tested by reality, Finally determine the optimal proportion of each microorganism, thus ensure the nutritional need in fruit tree each stage.
The preparation method of the organic composite fertilizer that concrete fruit tree of the present invention is special, the method includes walking as follows Rapid:
(1) preparation of organic matter decomposing inoculant
Strain quantity is respectively reached 108The bacillus subtilis (Bacillus subtilis) of individual/more than mL CICC10088, aspergillus oryzae (Aspergillus niger) CICC2436, whiterot fungi (Phanerochaete Chrysosporium) ACCC30414, the strain fermentating liquid of trichoderma reesei (Trichoderma reesei) ACCC30449 are pressed After the volume ratio mix homogeneously of 1.5～2.0:0.8～1.3:0.8～1.3:0.8～1.3, it is inoculated in and fills composite bacteria agent capable cultivation In the fermentation tank of base A, inoculum concentration is the 5% of composite bacteria agent capable culture medium A cumulative volume, cultivates 4～7 days, make in fermentation liquid at 30 DEG C Total bacteria count reaches 108Individual/mL, i.e. obtains organic matter decomposing inoculant；
Described composite bacteria agent capable culture medium A formula is: Semen Maydis flour 0.5～1.5g/L, corn stalk powder 15～17g/L, wheat bran 0.2～0.4g/L, soybean cake powder 0.2～0.4g/L, Vogel ' s mother solution 4mL/100mL, pH value 6.0～8.0；
The concrete preparation process of step () is:
1. actication of culture: described each strain is connected on slant medium；Bacillus subtilis 28～32 DEG C, pH is 7.2 ～7.4, incubation time is 1～3 day；Aspergillus oryzae cultivation temperature 25～28 DEG C, pH is 6.0～6.5, and incubation time is 4～7 days； Whiterot fungi cultivation temperature 25～28 DEG C, incubation time is 4～7 days；
2. first class inoculum is cultivated: be inoculated in respectively by described strain in the 50mL conical flask filling fluid medium, cultivates Base pH is 6.0～8.0, and conical flask puts into shaking table shaken cultivation, and temperature is 30 DEG C, vibration 24～48h；Use blood counting chamber meter Number, in bacterium solution, strain quantity respectively reaches 108Individual/mL；
3. second order fermentation: described strain is inoculated in respectively in the 1000mL conical flask filling fluid medium, culture medium PH 6.0～8.0, puts into shaking table vibration, and temperature is 30 DEG C, vibration 48～72h；Thus obtain strain quantity and respectively reach 108 Bacillus subtilis (Bacillus subtilis) CICC10088 of individual/more than mL, aspergillus oryzae (Aspergillus niger) CICC2436, whiterot fungi (Phanerochaete chrysosporium) ACCC30414, trichoderma reesei (Trichoderma reesei)ACCC30449。
Described slant culture based formulas is:
1. the slant culture based formulas that bacillus subtilis uses is: beef infusion broth 1.0L, peptone 10g, NaCl5.0g, Agar 20.0-20.5g, pH7.2-7.4, the preparation of beef infusion broth: lean beef cleans, chopping, weigh 550g and be soaked in 1375mL In water, soaking a night, filter, after filtering subpackage, 0.6kgf/cm2 40min is standby.
2. the slant culture based formulas that aspergillus oryzae uses is: 50Be wort agar, 50Be beerwort 1.0L, agar 15.0g, natural pH.
3. the slant culture based formulas of whiterot fungi is: PDA culture medium, removes the Rhizoma Solani tuber osi 200 grams of skin, is cut into small pieces, adds 1000 milliliters of water boils 30 minutes and filters off potato ball, filtrate complements to 1000 milliliters, adds glucose 20 grams, 15 grams of agar, Dissolve rear subpackage, 15 pounds of sterilizings 30 minutes；Natural pH.
4. the slant culture based formulas of trichoderma reesei: same to whiterot fungi.
Described fluid medium is that slant medium is not added with agar.
(2) puddle, ferment
Organic matter decomposing inoculant step () prepared is mixed in 5 ‰ ratios of fermented organic material gross mass, then It is blended into appropriate water, makes total moisture control about 60%, at fermentation vat through fermentation in 5-10 days, i.e. prepare the most fertile；
Described fermentation particularly as follows: put fermentation installation into by the fermented organic material of mixing and stirring, and venting is filled up in compacting Air, fermentation vat top covers layer of plastic film, upper cover sealing and fermenting, turns over buttress in good time, keeps the skin wet, by total moisture during turning Controlling about 60%, raw material is held agglomerating, and webs have water but do not drip, and looses one's grip and i.e. dissipate preferably.
In preferred technical scheme, the fermentation time of step (two) refers to: general 4-5 days summer, 6-7 days spring and autumn, winter 10 days seasons.
(3) deep processing
Carry out first fertilizer pulverizing, drying, by the most fertile mass percent, the specifically following nutrient of interpolation:
Nitrogen 1.0-3.0%, phosphorus 0.5-2.5%, potassium 1.0-3.0%；Calcium 0.2-0.5%, magnesium 0.2-0.5%；Boron 0.01- 0.04%, zinc 0.01-0.04%, ferrum 0.01-0.02%；Humic acid fertilizer 5.0～10.0%；
Under preferable case, the ratio adding nutrient is:
A. the adding proportion of a great number of elements (nitrogen, phosphorus, potassium): nitrogen 2.0%, phosphorus 1.0%, potassium 2.0%；
B. the adding proportion of moderate-element: calcium 0.5%, magnesium 0.5%；
C. the adding proportion of trace element: boron 0.02%, zinc 0.02%, ferrum 0.01%；
D. the adding proportion of ammonium humate: 5.0%.
(4) preparation of fruit tree special growth promotion composite bacteria agent capable
Strain quantity is respectively reached 108The streptomyces microflavus (Streptomyces microflavus) of individual/more than mL CGMCC4.891, colloid bacillus cereus (Bacillus mucilaginosus) CGMCC 1.153, aztobacter sp (Azotobacter chroococcum) CGMCC 1.142, bacillus megaterium (Bacillus megaterium) ACCC 10010, the strain fermentating liquid of bacillus subtilis (Bacillus subtilis) ACCC 01183, by (10～20%): (20 ～30%): (10～20%): (30～40%): as mix bacterium agent after the ratio mix homogeneously of (5～10%), be inoculated in Sheng Having in the fermentation tank of composite bacteria agent capable culture medium B, inoculum concentration is the 5% of composite bacteria agent capable culture medium B cumulative volume, composite bacteria agent capable culture medium The pH 6.5～7.5 of B, cultivates 4～7 days at 30 DEG C, makes total bacteria count in fermentation liquid reach 6～10 × 108Individual/mL, i.e. obtains fruit Tree special microorganism composite bacterial agent, pH is 6.8, and total living bacteria count is 9 × 108Individual/mL；
The formula of described composite bacteria agent capable culture medium B is: Semen Maydis powder 15～18g/100mL, Semen Glycines powder 5～8g/100mL, ferment Female cream 0.1～0.2g/100mL, KH2PO40.2～2g/100mL, K2HPO40.5～2g/100mL, MgSO4·7H2O 0.001 ～0.02g/100mL, NaCl 0.5～0.8g/100mL, (NH4)2SO40.001～0.01g/100mL, remaining is distilled water；
The detailed process of described step (four) is as follows:
1. inoculation: by described each strain (streptomyces microflavus, colloid bacillus cereus, aztobacter sp, huge spore bar Bacterium, bacillus subtilis) it is connected to respectively on slant medium；Streptomyces microflavus cultivation temperature is 25～30 DEG C, pH7.2～7.4, Incubation time 3～5 days；Colloid bacillus cereus (B. mucilaginocus) cultivation temperature 25～35 DEG C, pH is 7.5～8.0, and incubation time is 5 My god；Bacillus licheniformis cultivation temperature 25～30 DEG C, pH is 7.0～8.0, and incubation time is 5 days；Bacillus megaterium be 27～ 37 DEG C, pH is 6.5～7.5, and incubation time is 4 days；Bacillus subtilis cultivation temperature 27～37 DEG C, pH is 6.5～7.5, training The foster time is 4 days；
2. first class inoculum is cultivated: the strain described step 1 prepared is inoculated in by the amount of inoculating loop one ring respectively and fills phase Answering in the 50mL conical flask of fluid medium, medium pH is 6.5～7.5, and conical flask puts into shaking table vibration, and temperature is 30 DEG C, Vibration 24～48h；Employing blood counting chamber counts, and in bacterium solution, strain quantity respectively reaches 108Individual/mL；
3. second order fermentation: the strain described step 2 prepared is inoculated in the 1000mL filling fluid medium respectively by 5% In conical flask, medium pH 6.5～7.5, put into shaking table vibration, temperature is 30 DEG C, vibration 48～72h；Make strain quantity respectively Reach 108Individual/more than mL, thus prepare strain quantity and respectively reach 108Streptomyces microflavus (the Streptomyces of individual/more than mL Microflavus) CGMCC4.891, bacillus subtilis (Bacillus subtilis) ACCC 01183, bacillus megaterium (Bacillus megaterium) ACCC 10010, aztobacter sp (Azotobacter chroococcum) CGMCC 1.142, the strain fermentating liquid of colloid bacillus cereus (Bacillus mucilaginosus) CGMCC 1.153.
The slant culture based formulas used in described fruit tree complex microbial inoculum preparation process is:
1. the slant culture based formulas that colloid bacillus cereus uses is: KH2PO4 0.06g、MgSO4·7H2O 0.05g、
CaSO4·5H2O 0.004g, peptone 5g, Semen Maydis pulp (50% dry weight) 2.5g, lactose 15g, NaCl 4g, distillation Water 1000mL, agar 25g, pH 7.1；
2. the slant culture based formulas that aztobacter sp uses is:: KH2PO4 0.2g、K2HPO4 0.8g、MgSO4· 7H2O0.2g、CaSO4·2H2O 0.1g、Na2MoO4·2H2O trace, yeast extract 0.5g, mannitol 20g, FeCl3Trace, agar 15g, distilled water 1000mL, pH 7.2；
3. the slant culture based formulas that bacillus megaterium uses is: Carnis Bovis seu Bubali cream 3g, peptone 5g, agar 15g, NaCl 5g, distilled water 1000mL, pH 7.0；
4. the slant culture based formulas that bacillus subtilis uses is: Carnis Bovis seu Bubali cream 10g, peptone 10g, NaCl 5g, Fructus Vitis viniferae Sugar 10g, agar 15g, distilled water 1000mL, pH 7.0；
5. the slant culture based formulas that streptomyces microflavus uses is that: Gao Shi synthesizes an agar: soluble starch 20g, KNO31.0g, K2HPO40.5g, MgSO4·7H2O 0.5g, NaCl 0.5g, FeSO4·7H2O0.01g, agar 15.0g, distillation Water 1.0L, pH7.2-7.4.
Described liquid culture based formulas be slant medium be not added with agar prepare.
(5) 3～5 ‰ ratios interpolation step (four) of the fermentation materials gross mass obtained in step (three) deep processing obtain The special growth promotion composite bacteria agent capable of fruit tree obtain finished product, after material mix homogeneously, through granulator granulation or become powder, inspection Survey qualified after, packaging warehouse-in.
For technique scheme, in situations where it is preferred, step (one) described composite bacteria agent capable culture medium A formula is: beautiful Rice and flour 0.5g/L, corn stalk powder 15g/L, wheat bran 0.25g/L, soybean cake powder 0.25g/L, Vogel ' s mother solution 4mL/100mL, pH 7.0。
For technique scheme, in situations where it is preferred, step (one) described bacillus subtilis (Bacillus Subtilis) CICC10088, aspergillus oryzae (Aspergillus niger) CICC2436, whiterot fungi (Phanerochaete Chrysosporium) ACCC30414, trichoderma reesei (Trichoderma reesei) ACCC30449 strain fermentating liquid by 2: The ratio mix homogeneously of 1:1:1.
For technique scheme, in situations where it is preferred, step (two) described fermented organic material is the Semen Maydis pulverized Straw is mixed by 1.0～2.0:0.4～1.4 mass ratioes with animal wastes, and described animal wastes are usually chicken manure or cattle manure.
For technique scheme, in situations where it is preferred, the formula of step (three) described composite bacteria agent capable culture medium B is: Semen Maydis powder 16g/100mL, Semen Glycines powder 7g/100mL, yeast extract 0.1g/100mL, KH2PO4 1g/100mL、K2HPO4 1g/ 100mL、MgSO4·7H2O 0.01g/100mL、NaCl 0.6g/100mL、(NH4)2SO40.01g/100mL, remaining is distillation Water.
The technical scheme is that the unremitting effort through in recent years, in conjunction with the growth characteristic in fruit tree each period and right The demand of nutrient, notes regulating its contradiction with reproductive growth of nourishing and growing, and the biology finally developing a kind of high-efficiency high-quality has Machine is fertile.Its integrated use newest fruits such as microbial technique, chemical fertilizer industry and agricultural technology, are preferentially prepared for a kind of efficient Combine rich in organic, N P K nutrient and some material such as trace element, plant growth regulator, by Special process has ultimately formed the graininess nutrient source that the nutrition based on organic matter is reasonable, N P K ratio is suitable.This kind Fertilizer not only has high usage, the advantage of fertilizer efficiency period length, and has improvement soil, improves fruit quality, strengthens fruit tree and resists Inverse property, increases yield, reduces and pollutes, and stimulates the features such as growth and development of fruit tree, time and labour saving, has good social benefit and warp Ji benefit.
Beneficial effect
The special biological organic fertilizer of fruit tree of the present invention is to select the strain with specific function of becoming thoroughly decomposed to make microbial inoculum, Organic solid material fermentation is become thoroughly decomposed into the most fertile with this microbial inoculum, then add a great number of elements, moderate-element, trace in suitable ratio Element and other particular matters, then be equipped with effect and promote the fertilizer made by microbial inoculum of fruit tree growth accordingly.Decomposing agent contains There are substantial amounts of bacillus subtilis, aspergillus oryzae, trichoderma reesei, whiterot fungi, Geobacillus stearothermophilus, it is possible to produce cellulose The multiple enzyme such as enzyme, lignoenzyme, hemicellulase, waxiness element enzyme, pectase, collaborative by mesophilic bacteria and high temperature resistant bacterium All kinds of discarded SOLID ORGANIC material fermentation maturities are quickly become fertilizer, turn waste into wealth by effect, save resource, greatly reduce Environmental pollution；Add again all kinds of nutrient and the special growth promotion composite bacteria agent capable of fruit tree, by fruit tree is had special efficacy Beneficial microbe bacillus subtilis, colloid bacillus cereus, bacillus megaterium, Candida utilis, Bacillus licheniformis, big Interaction between bean root nodule bacteria, each strain of Lactobacillus plantarum, plays stimulation plant growing, alleviates the hardening soil, conditioned soil Earth ecological environment, the humus increasing soil and the effect such as beneficial microbe colony, disease resistance, thus reach volume increase, high yield Purpose.
Detailed description of the invention
The following non-limiting example of the present invention can make those of ordinary skill in the art that this is more fully understood Bright, but limit the present invention never in any form.Biomaterial used in embodiment all can be by being either commercially available.
Embodiment one: the preparation of the special organic composite fertilizer of fruit tree
The special organic composite fertilizer product of fruit tree of the present invention refers to utilize microbial technique, apolegamy to have specific becoming thoroughly decomposed The strain of function makes microbial inoculum, organic solid material fermentation is become thoroughly decomposed into the most fertile with this microbial inoculum, then be equipped with various nutrient and The bio-feritlizer made by growth promotion composite bacteria agent capable that fruit tree is special.Its detailed description of the invention is as follows:
(1) preparation of organic matter decomposing inoculant
Use bacillus subtilis (Bacillus subtilis) CICC10088, aspergillus oryzae (Aspergillus Niger) CICC2436, whiterot fungi (Phanerochaete chrysosporium) ACCC30414, trichoderma reesei (Trichoderma reesei) ACCC30449 etc. has the strain composite fermentation in appropriate proportion of the effect of efficiently becoming thoroughly decomposed and forms Decomposing microbial inoculum.
1. actication of culture: described each strain is connected on slant medium；Bacillus subtilis 28～32 DEG C, pH is 7.2 ～7.4, incubation time is 1～3 day；Aspergillus oryzae cultivation temperature 25～28 DEG C, pH is 6.0～6.5, and incubation time is 4～7 days； Whiterot fungi cultivation temperature 25～28 DEG C, incubation time is 4～7 days；
2. first class inoculum is cultivated: be inoculated in respectively by described strain in the 50mL conical flask filling fluid medium, cultivates Base pH is 6.0～8.0, and conical flask puts into shaking table shaken cultivation, and temperature is 30 DEG C, vibration 24～48h；Use blood counting chamber meter Number, in bacterium solution, strain quantity respectively reaches 108Individual/mL；
3. second order fermentation: described strain is inoculated in respectively in the 1000mL conical flask filling fluid medium, culture medium PH 6.0～8.0, puts into shaking table vibration, and temperature is 30 DEG C, vibration 48～72h；Strain quantity is made to respectively reach 108Individual/mL with On, by each strain by the mass percent mixing of described strain；
4. three grade fermemtation: respectively by bacillus subtilis (Bacillus subtilis) CICC10088, aspergillus oryzae (Aspergillus niger) CICC2436, whiterot fungi (Phanerochaete chrysosporium) ACCC30414, inner After the strain fermentating liquid of family name's Trichoderma spp. (Trichoderma reesei) ACCC30449 is in the ratio mix homogeneously of 2:1:1:1, inoculation In the fermentation tank filling composite bacteria agent capable culture medium, composite bacteria agent capable medium pH 6.0～8.0, inoculum concentration is 5%, trains at 30 DEG C Support 4～7 days, make bacterium number in fermentation liquid reach 108Individual/mL, i.e. obtains organic matter decomposing inoculant.
Described slant culture based formulas is:
1. the slant culture based formulas that bacillus subtilis uses is: plain agar: beef infusion broth 1.0L, peptone 10g, NaCl5.0g, agar 20.0-20.5g, pH7.2-7.4, the preparation of beef infusion broth: lean beef cleans, chopping, weigh 550g and soak In 1375mL water, soak a night, filter, filter 0.6kgf/cm after subpackage240min is standby.
2. the slant culture based formulas that aspergillus oryzae uses is: 50Be wort agar, 50Be beerwort 1.0L, agar 15.0g, natural pH.
3. the slant culture based formulas of whiterot fungi is: PDA culture medium, removes the Rhizoma Solani tuber osi 200 grams of skin, is cut into small pieces, adds 1000 milliliters of water boils 30 minutes and filters off potato ball, filtrate complements to 1000 milliliters, adds glucose 20 grams, 15 grams of agar, Dissolve rear subpackage, 15 pounds of sterilizings 30 minutes.Natural pH.
4. the slant culture based formulas of trichoderma reesei is: with whiterot fungi formula.
Described fluid medium is that slant medium is not added with agar.
Described composite bacteria agent capable culture medium prescription is: Semen Maydis flour 0.5g/L, corn stalk powder 15g/L, wheat bran 0.25g/L, bean Cake powder 0.25g/L, 4mL/100mL Vogel ' s mother solution, pH value 7.0.
(2) puddle, ferment
The corn straw that preparation 500kg pulverizes and 200kg cattle manure are as the initial organic matter material fermented, by having of preparing Machine material decomposing agent is blended in appropriate water by the 5 ‰ of fermented organic material gross mass, uniformly puddles with organic materials, raw material hands Holding agglomerating, webs have water but do not drip, and loose one's grip and i.e. dissipate (total moisture about 60%) preferably.The fermentation of mixing and stirring is had Fermentation installation put into by machine material, and venting air is filled up in compacting, and fermentation vat top covers layer of plastic film, upper cover sealing and fermenting, Turn over buttress in good time, keep the skin wet during turning, total moisture is controlled about 60%.Through fermentation in 10 days, i.e. prepare the most fertile.
(3) interpolation of all kinds of nutrients
After material fermentation completes, carry out pulverizing, drying by fertile dosage form requirement, then add all kinds of nutrient, including big Secondary element (nitrogen, phosphorus, potassium), moderate-element (calcium, magnesium), trace element (boron, zinc, ferrum), humic acid fertilizers are ammonium humate, press Fermentation materials Mass Calculation, concrete adding proportion is as follows:
A. the adding proportion of a great number of elements (nitrogen, phosphorus, potassium): nitrogen 2.0%, phosphorus 1.0%, potassium 2.0
B. the adding proportion of moderate-element: calcium 0.5%, magnesium 0.5%；
C. the adding proportion of trace element: boron 0.02%, zinc 0.02%, ferrum 0.01%；
D. the adding proportion of ammonium humate: 5.0%.
(4) preparation of fruit tree special growth promotion composite bacteria agent capable and interpolation
Use streptomyces microflavus (Streptomyces microflavus) CGMCC4.891, colloid bacillus cereus (Bacillus mucilaginosus) CGMCC 1.153, aztobacter sp (Azotobacter chroococcum) CGMCC 1.142, bacillus megaterium (Bacillus megaterium) ACCC 10010, bacillus subtilis (Bacillus Subtilis) as mix bacterium agent after ACCC 01183 etc. have the strain fermentating liquid mixing of special efficacy, through fermentation again The complex microbial inoculum of preparation.
Described fruit tree complex microbial inoculum preparation process is as follows:
1. inoculation: by described each strain (streptomyces microflavus, colloid bacillus cereus, aztobacter sp, huge spore bar Bacterium, bacillus subtilis) it is connected to respectively on slant medium；Streptomyces microflavus cultivation temperature is 25～30 DEG C, pH7.2～7.4, Incubation time 3～5 days；Colloid bacillus cereus (B. mucilaginocus) cultivation temperature 25～35 DEG C, pH is 7.5～8.0, and incubation time is 5 My god；Aztobacter sp cultivation temperature 25～30 DEG C, pH is 7.0～8.0, and incubation time is 5 days；Bacillus megaterium is 27 ～37 DEG C, pH is 6.5～7.5, and incubation time is 4 days；Bacillus subtilis cultivation temperature 27～37 DEG C, pH is 6.5～7.5, Incubation time is 4 days；
2. first class inoculum is cultivated: the strain described step 1 prepared is inoculated in by the amount of inoculating loop one ring respectively and fills phase Answering in the 50mL conical flask of fluid medium, medium pH is 6.5～7.5, and conical flask puts into shaking table vibration, and temperature is 30 DEG C, Vibration 24～48h；Employing blood counting chamber counts, and in bacterium solution, strain quantity respectively reaches 108Individual/mL；
3. second order fermentation: the strain described step 2 prepared is inoculated in the 1000mL filling fluid medium respectively by 5% In conical flask, medium pH 6.5～7.5, put into shaking table vibration, temperature is 30 DEG C, vibration 48～72h；Make strain quantity respectively Reach 108Individual/more than mL, thus prepared streptomyces microflavus fermentation liquid, colloid bacillus cereus (B. mucilaginocus) fermentation liquid, brown are spherical Nitrogen-fixing bacteria fermentation liquid, bacillus megaterium fermentation liquid and fermentation of bacillus subtilis liquid.
Again ferment after each strain is mixed by mass percentage: streptomyces microflavus fermentation liquid 15%, colloid spore Bacillus (B. mucilaginocus) fermentation liquid 25%, bacillus megaterium fermentation liquid 30%, aztobacter sp fermentation liquid 20%, hay bud Spore bacillus fermentation liquid 10%.
4. three grade fermemtation: the mixed bacterium solution described step 3 prepared is inoculated in and fills sending out of composite bacteria agent capable culture medium In ferment tank, composite bacteria agent capable medium pH 6.5～7.5, inoculum concentration is the 5% of composite bacteria agent capable culture medium gross mass, cultivates at 30 DEG C 4～7 days, bacterium number in fermentation liquid is made to reach 6～10 × 108Individual/mL, i.e. obtains fruit tree special microorganism composite bacterial agent, and pH is 5.0, bacterium number content all reaches 109Individual/mL.
Described slant culture based formulas is:
1. the slant culture based formulas that colloid bacillus cereus uses is: KH2PO4 0.06g、MgSO4·7H2O 0.05g、
CaSO4·5H2O 0.004g, peptone 5g, Semen Maydis pulp (50% dry weight) 2.5g, lactose 15g, NaCl 4g, distillation Water 1000mL, agar 25g, pH 7.1；
2. the slant culture based formulas that aztobacter sp uses is:: KH2PO4 0.2g、K2HPO4 0.8g、MgSO4· 7H2O 0.2g、CaSO4·2H2O 0.1g、Na2MoO4·2H2O trace, yeast extract 0.5g, mannitol 20g, FeCl3Trace, fine jade Fat 15g, distilled water 1000mL, pH 7.2；
3. the slant culture based formulas that bacillus megaterium uses is: Carnis Bovis seu Bubali cream 3g, peptone 5g, agar 15g, NaCl 5g, distilled water 1000mL, pH 7.0；
4. the slant culture based formulas that bacillus subtilis uses is: Carnis Bovis seu Bubali cream 10g, peptone 10g, NaCl 5g, Fructus Vitis viniferae Sugar 10g, agar 15g, distilled water 1000mL, pH 7.0；
5. the slant culture based formulas that streptomyces microflavus uses is that: Gao Shi synthesizes an agar: soluble starch 20g, KNO31.0g, K2HPO40.5g, MgSO4·7H2O 0.5g, NaCl 0.5g, FeSO4·7H2O0.01g, agar 15.0g, distillation Water 1.0L, pH7.2-7.4.
Described liquid culture based formulas be slant medium be not added with agar prepare.
Described composite bacteria agent capable culture medium prescription is: Semen Maydis powder 2～20g/100mL, Semen Glycines powder 1～10g/100mL, yeast extract 0.05～0.2g/100mL, KH2PO40.2～2g/100mL, K2HPO40.5～2g/100mL, MgSO4·7H2O 0.001～ 0.02g/100mL, NaCl 0.5～0.8g/100mL, (NH4)2SO40.001～0.01g/100mL, remaining is distilled water.
The growth promotion composite bacteria agent capable that fruit tree is special, mix homogeneously again is added in 4 ‰ ratios of fermentation materials gross mass.
(5) pelletize or one-tenth powder, detect, pack, put in storage
After material mix homogeneously, through granulator granulation or become powder, detect qualified after, packaging warehouse-in.
Embodiment two: fruit tree special organic composite fertilizer application effect on apple tree
1. test objective
The special organic composite fertilizer of fruit tree is the raw for fruit tree of the up-to-date development of DaLian Sanke Bio-engineering Co., Ltd. The long bio-feritlizer having special efficacy, for its impact that Apple Growth is grown clear and definite, 4-9 month in 2015 my company for Apple tree has carried out field plot trial.
2. experimental condition: test site is Yongning town, Wafangdian, Daliang City, and plot is representative.Physical features is smooth, fertility Medium, establishing in large scale apple tree.
3. test material and method
Experimental cultivar: Fuji apple
Test method: test is set to two groups, uses random alignment, repeats for three times respectively, each experimental plot area 667 Square metre.
Two groups of described process:
1. matched group, conventional fertilizer application；2. test group, uses in strict accordance with organic composite fertilizer product operation instruction.
Through actual survey, within 2015, fruit tree period of duration natural conditions are preferable, without special vile weather, beneficially apple tree Growth.
4. investigation records method gathers investigation BAIGUO weight, per mu yield in the ripe apples phase, and samples at soil 0-20cm, Detection soil with organic matter content, micro organism quantity (hundred million/g) and pH change.
5. result of the test and analysis
5.1 use the impact on apple tree yield of the fruit tree special organic composite fertilizer
The special organic composite fertilizer of fruit tree used by table 1 affects data to apple tree yield
Process BAIGUO weight (kg) Yield (kg)/667 square metre Rate of growth (%)
Matched group 28.5 4399.2 --
Test group 31.6 5496.8 24.9
As it can be seen from table 1 test group Fructus Mali pumilae BAIGUO anharmonic ratio matched group height 3.1kg, per mu yield is also significantly greater than matched group, Rate of growth reaches 24.9%, shows that using the special organic composite fertilizer of fruit tree can significantly improve the yield of Fructus Mali pumilae.
5.2 use the impact on apple tree ground soil physico-chemical property of the fruit tree special organic composite fertilizer
The special organic composite fertilizer of fruit tree used by table 2 affects number to soil organic matter content, micro organism quantity and pH According to
Process The content of organic matter (%) Micro organism quantity (hundred million/g) pH
Matched group 2.3 6.8 5.9
Test group 3.1 15.6 6.5
As can be seen from Table 2, the content of organic matter of test group soil and micro organism quantity are all significantly higher than matched group, High by 0.8%, 8.8 hundred million/g respectively, the pH of soil also improves 0.6, shows that using the special organic composite fertilizer of fruit tree can change Good soil, improves organic matter and the content of microorganisms of soil, improves the acid-base value of soil so that soil environment apple tree preferably Growth
Embodiment three: fruit tree special organic composite fertilizer application effect on cherry tree
1. test objective
The special organic composite fertilizer of fruit tree is the raw for fruit tree of the up-to-date development of DaLian Sanke Bio-engineering Co., Ltd. The long bio-feritlizer having special efficacy, for its impact on cherry tree growth promoter clear and definite, 4-9 month in 2015 my company for Cherry tree has carried out field plot trial.
2. experimental condition: test site is Lvshunkou District, Daliang City, and plot is representative.Physical features is smooth, middle fertility, Establishing in large scale cherry tree.
3. test material and method
Experimental cultivar: red lantern Fructus Pruni pseudocerasi
Test method: test is set to two groups, uses random alignment, repeats for three times respectively, each experimental plot area 667 Square metre.
Two groups of described process:
1. matched group, conventional fertilizer application；2. test group, uses in strict accordance with organic composite fertilizer product operation instruction.
Through actual survey, within 2015, fruit tree period of duration natural conditions are preferable, without special vile weather, beneficially cherry tree Growth
4. investigation records method the Fructus Pruni pseudocerasi period of maturation gather investigation single plant yield, per mu yield, and in each experimental plot with Machine takes 20 Fructus Pruni pseudocerasis and analyzes its total acid, total sugar and Vc content.Total acid, total sugar content titration measuring, Vc content takes molybdenum blue Colorimetric method for determining.
5. result of the test and analysis
5.1 use the impact on cherry tree yield of the fruit tree special organic composite fertilizer
Fruit tree organic composite fertilizer used by table 1 affects data to cherry tree yield
Process Yield (kg)/strain Yield (kg)/667 square metre Rate of growth (%)
Matched group 44.2 1944.8 --
Test group 51.6 2270.4 16.7
From table 1 data it will be seen that test group each tree yield increased group height 7.4kg, per mu yield is higher than matched group 325.6kg, rate of growth reaches 16.7%, shows that using the special organic composite fertilizer of fruit tree can significantly improve the product of cherry tree Amount.
5.2 use the impact on Fructus Pruni pseudocerasi quality of the fruit tree special organic composite fertilizer
The special organic composite fertilizer of fruit tree used by table 2 affects data to Fructus Pruni pseudocerasi total sugar, total acid and Vc content
Process Total sugar (%) Total acid (%) Vc(mg/100g)
Matched group 8.423 1.123 8.456
Test group 11.320 1.040 18.250
From table 2 data it is observed that the Fructus Pruni pseudocerasi total sugar content of test group is significantly higher than matched group, total acid is less than comparison Group, Vc content also dramatically increases, and adds 9.794.Show that using the special organic composite fertilizer of fruit tree can significantly improve Fructus Pruni pseudocerasi The quality of fruit, increases the mouthfeel of Fructus Pruni pseudocerasi.
Conclusion
By finding the result of the test of apple tree and cherry tree, using the special organic composite fertilizer of fruit tree can be notable Improve apple tree and the yield of cherry tree and quality, significantly improve economic benefit.And it is raw to use the special organic composite of fruit tree Thing fertilizer can also improve soil, increases soil organic matter content and acid-base value so that it is be more beneficial for the growth of fruit tree.Therefore, really Set special organic composite fertilizer and can expand demonstration area in all kinds of production of fruit trees are planted, and carry out spread use.

Claims (6)

1. the organic composite fertilizer that a planting fruit-trees is special, it is characterised in that the preparation method bag of described organic composite fertilizer Include:
(1) preparation of organic matter decomposing inoculant
Strain quantity is respectively reached 108Bacillus subtilis (Bacillus subtilis) CICC10088 of individual/more than mL, Aspergillus oryzae (Aspergillus niger) CICC2436, whiterot fungi (Phanerochaete chrysosporium) ACCC30414, trichoderma reesei (Trichoderma reesei) ACCC30449 strain fermentating liquid by 1.5～2.0:0.8～ After the ratio mix homogeneously of 1.3:0.8～1.3:0.8～1.3, it is inoculated in the fermentation tank filling composite bacteria agent capable culture medium A, connects The amount of kind is the 5% of composite bacteria agent capable culture medium A cumulative volume, cultivates 4～7 days, make total bacteria count in fermentation liquid reach 10 at 30 DEG C8Individual/ ML, i.e. obtains organic matter decomposing inoculant；
Described composite bacteria agent capable culture medium A formula is: Semen Maydis flour 0.5～1.5g/L, corn stalk powder 15～17g/L, wheat bran 0.2～ 0.4g/L, soybean cake powder 0.2～0.4g/L, 4mL/100mL Vogel ' s mother solution, pH value 6.0～8.0；
(2) puddle, ferment
Organic matter decomposing inoculant step () prepared is in 5 ‰ ratio mixing of fermented organic material gross mass, then is blended into Appropriate water, makes total moisture control about 60%, at fermentation vat through fermentation in 5-10 days, i.e. prepares the most fertile；
(3) deep processing
Carry out first fertilizer pulverizing, drying, by the most fertile mass percent, the specifically following nutrient of interpolation:
Nitrogen 1.0-3.0%, phosphorus 0.5-2.5%, potassium 1.0-3.0%；Calcium 0.2-0.5%, magnesium 0.2-0.5%；Boron 0.01- 0.04%, zinc 0.01-0.04%, ferrum 0.01-0.02%；Humic acid fertilizer 5.0～10.0%；
(4) preparation of fruit tree special growth promotion composite bacteria agent capable
Strain quantity is respectively reached 108The streptomyces microflavus (Streptomyces microflavus) of individual/more than mL CGMCC4.891, colloid bacillus cereus (Bacillus mucilaginosus) CGMCC 1.153, aztobacter sp (Azotobacter chroococcum) CGMCC 1.142, bacillus megaterium (Bacillus megaterium) ACCC 10010, the strain fermentating liquid of bacillus subtilis (Bacillus subtilis) ACCC 01183, by (10～20%): (20 ～30%): (10～20%): (30～40%): as mix bacterium agent after the ratio mix homogeneously of (5～10%), be inoculated in Sheng Having in the fermentation tank of composite bacteria agent capable culture medium B, inoculum concentration is the 5% of composite bacteria agent capable culture medium B cumulative volume, composite bacteria agent capable culture medium The pH 6.5～7.5 of B, cultivates 4～7 days at 30 DEG C, makes total bacteria count in fermentation liquid reach 6～10 × 108Individual/mL, i.e. obtains fruit Tree special microorganism composite bacterial agent, pH is 6.8, and total living bacteria count is 9 × 108Individual/mL；
The formula of described composite bacteria agent capable culture medium B is: Semen Maydis powder 15～18g/100mL, Semen Glycines powder 5～8g/100mL, yeast extract 0.1～0.2g/100mL, KH2PO40.2～2g/100mL, K2HPO40.5～2g/100mL, MgSO4·7H2O 0.001～ 0.02g/100mL, NaCl 0.5～0.8g/100mL, (NH4)2SO40.001～0.01g/100mL, remaining is distilled water；
(5) 3～5 ‰ ratios of the fermentation materials gross mass obtained in step (three) deep processing add what step (four) obtained The growth promotion composite bacteria agent capable acquisition finished product that fruit tree is special.
The preparation method of the biological organic fertilizer that fruit tree the most according to claim 1 is special, it is characterised in that step (one) institute Stating composite bacteria agent capable culture medium A formula is: Semen Maydis flour 0.5g/L, corn stalk powder 15g/L, wheat bran 0.25g/L, soybean cake powder 0.25g/ L, 4mL/100mL Vogel ' s mother solution, pH 7.0.
The preparation method of the biological organic fertilizer that fruit tree the most according to claim 1 is special, it is characterised in that step (one) institute State bacillus subtilis (Bacillus subtilis) CICC10088, aspergillus oryzae (Aspergillus niger) CICC2436, The strain fermentating liquid of whiterot fungi (Phanerochaete chrysosporium) ACCC30414 is mixed all in the ratio of 2:1:1:1 Even.
The preparation method of the biological organic fertilizer that fruit tree the most according to claim 1 is special, it is characterised in that step (two) institute Stating fermented organic material is that the corn straw pulverized is mixed by 1.0～2.0:0.4～1.4 mass ratioes with animal wastes.
The preparation method of the biological organic fertilizer that fruit tree the most according to claim 1 is special, it is characterised in that step (three) institute Stating nutrient is nitrogen 2.0%, phosphorus 1.0%, potassium 2.0%；Calcium 0.5%, magnesium 0.5%；Boron 0.02%, zinc 0.02%, ferrum 0.01%；Humic acid fertilizer 5.0%, humic acid fertilizer is ammonium humate or potassium humate.
The preparation method of the biological organic fertilizer that fruit tree the most according to claim 1 is special, it is characterised in that step (three) institute The formula stating composite bacteria agent capable culture medium B is: Semen Maydis powder 16g/100mL, Semen Glycines powder 7g/100mL, yeast extract 0.1g/100mL, KH2PO4 1g/100mL、K2HPO4 1g/100mL、MgSO4·7H2O 0.01g/100mL、NaCl 0.6g/100mL、(NH4)2SO40.01g/100mL, remaining is distilled water.
CN201610371039.3A 2016-05-30 2016-05-30 Method for preparing organic compound biological fertilizer special for fruit trees Pending CN106045677A (en)

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CN106543023A (en) * 2016-11-04 2017-03-29 湖北宇隆茶业有限责任公司 The processing method of the extracting method and tealeaf residue of tea polyphenols and theanine in tea old leaf
CN106565346A (en) * 2016-11-09 2017-04-19 凤台县余跃蔬菜专业合作社 Fertilizer additive for improving soil properties in tomato plantation and preparation method of fertilizer additive
CN107155772A (en) * 2017-05-27 2017-09-15 金寨县果兴果业农业科技开发有限公司 Chestnut wood Cultivate administration method
CN110963832A (en) * 2018-09-29 2020-04-07 镇江市水木年华现代农业科技有限公司 Biological organic compound fertilizer and preparation method thereof
CN112979385A (en) * 2019-12-13 2021-06-18 山西晋嘉潍生物科技有限公司 Method for preparing biological solid organic fertilizer special for fruit trees
CN114480162A (en) * 2021-12-07 2022-05-13 西南林业大学 Biological agent for preventing and treating camellia yunnanensis rot and application thereof

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CN104692866A (en) * 2013-12-06 2015-06-10 大连三科生物工程有限公司 Biological organic fertilizer
CN104862247A (en) * 2015-04-30 2015-08-26 山西师范大学 Liquid medium of composite microbial agent and preparation method thereof

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CN102199049A (en) * 2010-12-15 2011-09-28 沈阳科丰牧业科技有限公司 Microbial organic manure and preparation method thereof
CN104692866A (en) * 2013-12-06 2015-06-10 大连三科生物工程有限公司 Biological organic fertilizer
CN104862247A (en) * 2015-04-30 2015-08-26 山西师范大学 Liquid medium of composite microbial agent and preparation method thereof

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CN106543023A (en) * 2016-11-04 2017-03-29 湖北宇隆茶业有限责任公司 The processing method of the extracting method and tealeaf residue of tea polyphenols and theanine in tea old leaf
CN106565346A (en) * 2016-11-09 2017-04-19 凤台县余跃蔬菜专业合作社 Fertilizer additive for improving soil properties in tomato plantation and preparation method of fertilizer additive
CN107155772A (en) * 2017-05-27 2017-09-15 金寨县果兴果业农业科技开发有限公司 Chestnut wood Cultivate administration method
CN110963832A (en) * 2018-09-29 2020-04-07 镇江市水木年华现代农业科技有限公司 Biological organic compound fertilizer and preparation method thereof
CN112979385A (en) * 2019-12-13 2021-06-18 山西晋嘉潍生物科技有限公司 Method for preparing biological solid organic fertilizer special for fruit trees
CN114480162A (en) * 2021-12-07 2022-05-13 西南林业大学 Biological agent for preventing and treating camellia yunnanensis rot and application thereof
CN114480162B (en) * 2021-12-07 2022-09-20 西南林业大学 Biological agent for preventing and treating camellia yunnanensis rot and application thereof

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Application publication date: 20161026

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