CN101463334B - Fermentation liquor composition for preparing biological organic fertilizer and preparation and use thereof - Google Patents

Fermentation liquor composition for preparing biological organic fertilizer and preparation and use thereof Download PDF

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Publication number
CN101463334B
CN101463334B CN2009100366182A CN200910036618A CN101463334B CN 101463334 B CN101463334 B CN 101463334B CN 2009100366182 A CN2009100366182 A CN 2009100366182A CN 200910036618 A CN200910036618 A CN 200910036618A CN 101463334 B CN101463334 B CN 101463334B
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fermentation liquor
fertilizer
liquor composition
fermentation
subtilis
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CN101463334A (en
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肖相政
廖宗文
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South China Agricultural University
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South China Agricultural University
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Abstract

The invention discloses a fermentation liquor composition used for preparing microbial organic fertilizer, which comprises the components based on the weight percentage: 60-70% of bacillus subtilis fermentation liquor, 15-20 % of bacillus pumilus fermentation liquor and 15-20 % of pseudomonas fluorescens fermentation liquor. The invention also provides a preparation method of the fermentation liquor composition, which comprises: bacillus subtilis, bacillus pumilus and the pseudomonas fluorescens are respectively fermented separately, and then the obtained fermentation liquors are mixed. The strain of the fermentation liquor composition has optimal combination, so as to realize versatility. The selected strain not only has effect of growth promotion, but also has antagonism effect for multiple plant soil-borne diseases. The bacillus subtilis and the pseudomonas fluorescens in the product are plant rhizosphere growth-promoting bacteria which can stimulate and induce the systemic resistance of the crops, improve the self immunity and enhance disease resistance.

Description

A kind of fermentation liquor composition of preparing biological organic fertilizer and its production and application that is used to
Technical field
The present invention relates to a kind of fermentation liquor composition of preparing biological organic fertilizer and its production and application that is used to, belong to agricultural biological technical field.
Background technology
Biological organic fertilizer be meant microorganisms with specific functions with main be source and the fertilizer that has microbial fertilizer and fertilizer effect through the class that harmless treatment, the organic materials that becomes thoroughly decomposed are composited concurrently with the plant and animal residues.Compare with common fertilizer, the technology content that biological organic fertilizer is produced is higher relatively, except in the process of becoming thoroughly decomposed, adding the biological mattress agent that promotes organic material composting, decomposition, with outside the purpose such as realize that orientation is become thoroughly decomposed, in product, also need add microorganism, to promote the action effect of product with specific function.
Biological organic fertilizer has obtained development fast at present, and the manufacturing enterprise more than 120 that obtains the product registration card in Ministry of Agriculture's registration is tame, and annual production has possessed certain industrial scale more than 2,000,000 tons, is becoming a new highlight of agricultural materials market.The feces of livestock and poultry of plant or agricultural crop straw etc. were as starting material near the corporate boss will utilize, and also comprised the offal, domestic refuse of some fermentation industries etc. simultaneously.Aspect zymotechnique, adopt the slot type fermentation method of banking up more, other fermentation process has also obtained application aborning as bank up fermentation method, fermenter fermentation method, gas-tight silo formula fermentation method, tower fermentation method etc. of level land.Aspect the aftertreatment of fermentation materials, most of enterprises add function yeast and carry out composite, setting, and product forms is based on pulvis, in addition, have 1/3rd product to adopt agglomeration technique approximately in last handling process, mainly are the disk agglomeration techniques.
Microbial strains is the core of microbial fertilizer product, in process of production, generally there are 2 links to relate to the use of microorganism, the one, adding promotes that material decomposes, becoming thoroughly decomposed has the mattress agent of becoming thoroughly decomposed of deodorization functions concurrently in the digest process, they are many is made up of composite microbial system, and common bacterial classification has photosynthetic bacterium, milk-acid bacteria, yeast, actinomycetes, mould, wooden mould, head mold etc.; The 2nd, the function yeast that after material becomes thoroughly decomposed, adds, generally based on vinelandii, phosphorus-solubilizing bacteria, silicate bacteria, milk-acid bacteria, pseudomonas, bacillus, actinomycetes etc., the specific fertilizer effect of performance in product.
Biological organic fertilizer integrates the advantage of bio-fertilizer and fertilizer, has both helped the agricultural-food increasing both production and income, but again fertilizing soil, improve the soil micro-ecosystem system, reduce the mineral manure consumption, improve quality of agricultural product.From whole agricultural industry and fertilizer market development situation, biological organic fertilizer should become a developing direction of agricultural fertilizer really.But this series products still is in the marketing stage at present, also there are many problems on each link of using from the production of product to the field, especially on product technology content, microorganism is the technological core of product, the screening of bacterial classification, composite etc. very important for the biological organic complex fertilizer effect, all there are shortcomings such as bacterial classification function singleness, product content of microorganisms are low product before the appearance in quite a few, does not reach the quality product requirement.
Summary of the invention
Technical problem to be solved by this invention is the deficiency that remedies prior art, purpose be to provide a kind of be used to prepare biological organic fertilizer, the various plants soil-borne disease is had fermentation liquor composition antagonistic effect, that can excite system's resistance of inducing crop to improve autoimmunity, enhance disease resistance.
Another object of the present invention provides the preparation method of above-mentioned fermentation liquor composition.
A further object of the invention provides the application of above-mentioned fermentation liquor composition in the preparation biological organic fertilizer.
The present invention is achieved through the following technical solutions above-mentioned purpose:
A kind of fermentation liquor composition that is used to prepare biological organic fertilizer is made up of following components in weight percentage: fermentation of bacillus subtilis liquid 60~70%, bacillus pumilus fermented liquid 15~20%, Pseudomonas fluorescens fermented liquid 15~20%.
Described fermentation of bacillus subtilis liquid is the mixture of the different fermentation of bacillus subtilis liquid of a bacillus subtilis fermented liquid or two strains; The preferred equal proportion of described two bacillus subtilis fermented liquids is mixed.
Also can add stablizer in the fermentation liquor composition of the present invention, the preferred methyl p-hydroxybenzoate of stablizer; The consumption of described stablizer is 0.1 of fermentation liquor composition gross weight~0.2 ‰.
The preparation method of above-mentioned fermentation liquor composition is with the fermentation separately respectively of subtilis, bacillus pumilus, Pseudomonas fluorescens, the fermented liquid that obtains is mixed again, and can add stablizer during mixing.Subtilis forms gemma, live bacterial count 〉=5 * 10 during fermentation termination more than 85% 8Cfu/mL, bacillus pumilus forms gemma, live bacterial count 〉=1 * 10 more than 85% 9Cfu/mL, Pseudomonas fluorescens fermentation live bacterial count 〉=1 * 10 9Cfu/mL.
The present invention also provides the application of above-mentioned fermentation liquor composition in the preparation biological organic fertilizer, is to add described fermentation liquor composition in the fertilizer that the nature of banking up becomes thoroughly decomposed, and addition is to add 150~250mL fermentation liquor composition, mixing in every 1000g fertilizer.Product behind the mixing directly packs or makes the granular biological fertilizer.
Described fertilizer is become thoroughly decomposed and is formed by the dry branches and fallen leaves organic waste materials of the collecting nature of banking up, and pulverizes 1~2mm sieve after becoming thoroughly decomposed, and its water content is less than 20%, and organic content is greater than 30%, total nutrient N+P 2O 5+ K 2O% is greater than 4%.
The present invention compared with prior art has following advantage and beneficial effect:
1) this product bacterial classification Combinatorial Optimization has been realized multifunction.Not only have short (fertilizer) effect of giving birth in the selected bacterial strain, main is that the various plants soil-borne disease is had antagonistic effect;
2) subtilis A, Pseudomonas fluorescens are the short living bacterium (PGPR) of plant rhizosphere in this product, can excite system's resistance of inducing crop, improve autoimmunity, enhance disease resistance;
3) in this product preparation method by improving zymotechnique, improved the fermentation level of each compatibility bacterial strain, making in the product effectively, the bacterium number improves greatly, thereby guaranteed that product is urged performance living, antagonistic effect in the application process, because the effectively raising of bacterium quantity in the product, guaranteed the chemical fertilizer product that makes with the competition of indigenous microorganism in get the mastery, effectively occupy ecological site; The increase of viable count has simultaneously guaranteed the quality product in the commodity validity period;
4) evidence is used the chemical fertilizer that this product makes multiple soil-borne diseases such as bacterial wilt of tomato, joint cucurbit wilt, pepper ralstonia solanacearum, banana blight is had good prevention effect, and promotes crop yield to increase;
5) use the chemical fertilizer product that this product makes and in its production process, made full use of biomass waste resources such as dry branches and fallen leaves, improved the utilization ratio of resource greatly;
6) interpolation of stablizer can also further guarantee the functional bacterial content that validity period is interior in the product of the present invention.
Embodiment
Below further specify technical scheme of the present invention by specific embodiment.
Embodiment 1
The subtilis A that does not have antagonistic action with a strain and relevant bacterial strain ferments separately, its fermented liquid mixes with bacillus pumilus fermented liquid 15%, Pseudomonas fluorescens fermented liquid 15% with 70% ratio, make fermentation liquor composition, in the fertilizer of getting ready, fully adsorb mixing, make the functional biological fertilizer in the ratio of 150~250mL fermentation liquor composition/1000g fertilizer.
Present embodiment gained functional biological fertilizer has antagonism and the short fruit of coming into force to the various plants soil-borne disease.
Embodiment 2
1, function yeast is fermented separately
Subtilis A (Bacillus subtilis) 1.1630, subtilis B (Bacillussubtilis1.1468), bacillus pumilus (Bacillus pumilus1.937) and Pseudomonas fluorescens (Pseudomonas fluorescens) GIM1.49 are all available from microbial strains preservation center, Guangdong Microbes Inst.
The fermentation condition of each bacterial strain is as follows:
1) subtilis A (subtilis 1.1630)
Fermention medium: starch 0.5% sucrose 0.8% soybean cake powder 0.3%K 2HPO 4.3H 2O 0.2%MgSO 4.7H 2O 0.06% ammonium sulfate 0.04% lime carbonate 0.2% yeast extract paste 0.03%
30~31 ℃ of time 30~36h of culture condition: pH 7.0~7.5 temperature
2) subtilis B (subtilis 1.1468)
Fermention medium: starch 0.5% sucrose 0.3% soybean cake powder 0.1%K 2HPO 4.3H 2O 0.1%MgSO 4.7H 2O 0.1% ammonium sulfate 0.08% lime carbonate 0.2% yeast extract paste 0.1%
30~34 ℃ of time 30~36h of culture condition: pH 7.2~7.5 temperature
3) bacillus pumilus 1.937
Fermention medium: starch 0.4% sucrose 0.2% soybean cake powder 0.1%K 2HPO 4.3H 2O 0.1%KH 2PO 4.12H 2O 0.1% lime carbonate 0.1% yeast extract paste 0.04% ferrous sulfate 0.002% manganous sulfate 0.002%
30~32 ℃ of time 24~28h of culture condition: pH 7.0~7.5 temperature
4) Pseudomonas fluorescens GIM1.49
Fermention medium: extractum carnis 0.5% peptone 1% sodium-chlor 0.5%
25~30 ℃ of time 24~28h of culture condition: pH 7.0~7.2 temperature
Selected four kinds of function stems do not have antagonistic action each other through the antagonistic experiment proof.
Through above-mentioned fermentation, two bacillus subtilis form gemma during fermentation termination more than 85%, and live bacterial count is 〉=5 * 10 8Cfu/mL; Bacillus pumilus forms gemma more than 85%, live bacterial count is 〉=1 * 10 9Cfu/mL; The Pseudomonas fluorescens live bacterial count is 〉=1 * 10 9Cfu/mL.
2, press the independent fermented liquid of 30%+30%+20%+20% mixed subtilis A, subtilis B, bacillus pumilus and Pseudomonas fluorescens; The stablizer methyl p-hydroxybenzoate that adds fermented liquid quality 0.2 ‰ simultaneously.
3, dry branches and fallen leaves is banked up after nature becomes thoroughly decomposed, and pulverizes the 2mm sieve, and its water content is 10~20%, and organic content is 〉=30%, total nutrient (N+P 2O 5+ K 2O) % is greater than 4.0%;
4, fermentation liquor composition and the fertilizer of getting ready are adsorbed by 20%, thorough mixing is even, and the product after the absorption directly packs or the granular biological fertilizer is made in granulation.
Embodiment 3
The fertilizer that embodiment 2 makes is used for following experiment:
1, the effect of functional biological fertilizer on capsicum
Capsicum is field greenhouse cultivation, morbidity for many years in this test.
Fertilizer amount and usage: 100kg/ mu, make base manure when capsicum is transplanted seedlings and use (conversion is the 20g/ strain).
The result: the contrast capsicum that do not process is most of withered, does not have output, and the processing of having used biological organic fertilizer is grown normally substantially, does not see morbidity.
2, the effect of functional biological fertilizer on potted plant tomato:
This test is that the solarium is potted plant, and potted plant selected soil is the vegetable garden soil of throughout the year falling ill.
Fertilizer amount and usage: 14.3g/ kilogram soil, base is executed when transplanting seedlings.
The result: 1) the inoculation Ralstonia solanacearum is after 40 days, and the whole morbidities of contrast of not executing biological organic fertilizer are withered, only have indivedual appearance slightly to fall ill and handle.
2) the period of disease sampling detects the quantity of pathogenic bacteria in the soil, and contrast is 2.5 * 10 7Cfu/ restrains dry ground, and the processing of fertilising only is 3.7 * 10 6Cfu/ restrains dry ground, and difference is very obvious, illustrate that pathogenic bacteria quantity obviously reduces in the soil of fertilising back, thereby sickness rate reduces.
3, the functional biological fertilizer is to the prevention effect of joint cucurbit wilt
This test is the land for growing field crops application test, and the place is farm, Danzhou, Hainan.
Fertilizer amount and usage: the functional biological fertilizer is made base manure and is used, and establishes two different amounts, is respectively 100g/ strain, 150g/ strain, adds contrast totally three processing, and every processing three times repeats, and every to repeat to select 10 strains be a sub-district.
The result: two kinds of different amounts all have prevention effect to the joint cucurbit wilt, see Table 1, and effect was better than the 100g/ strain when every in this test strain consumption was 150g.
Table 1 functional biological fertilizer is to the prevention effect of joint cucurbit wilt
When using method of the present invention, should also be noted that following item:
Under the lower situation of the fertilizer moisture content that is used to adsorb, can adopt the fermentation liquor composition and the fertilizer that becomes thoroughly decomposed to adsorb by 25%, and under the fertilizer moisture content condition with higher, generally by 15% absorption; And the composition of fermentation liquor composition mainly is to decide on applicable cases, if use the more serious plot of morbidity, then can suitably increase the ratio of two bacillus subtilis, each is with 35%, and bacillus pumilus and Pseudomonas fluorescens are respectively with 15%, otherwise, generally press the 30%+30%+20%+20% preferred version and mix.

Claims (7)

1. fermentation liquor composition that is used to prepare biological organic fertilizer, be made up of following components in weight percentage: the fermented liquid of subtilis A (Bacillus subtilis) 1.1630, subtilis B (Bacillus subtilis1.1468), bacillus pumilus (Bacillus pumilus1.937) and Pseudomonas fluorescens (Pseudomonas fluorescens) GIM1.49 is according to the mixed of 30%+30%+20%+20%.
2. fermentation liquor composition as claimed in claim 1 is characterized in that also adding in the described fermentation liquor composition stablizer of fermented liquid gross weight 0.1~0.2 ‰.
3. composition as claimed in claim 2 is characterized in that described stablizer is a methyl p-hydroxybenzoate.
4. the preparation method of the described fermentation liquor composition of claim 1 is with the fermentation separately respectively of subtilis A, subtilis B, bacillus pumilus, Pseudomonas fluorescens, the fermented liquid that obtains is mixed again.
5. the preparation method of the described fermentation liquor composition of claim 2 is with the fermentation separately respectively of subtilis, bacillus pumilus, Pseudomonas fluorescens, the fermented liquid that obtains is mixed again, and adds stablizer, mixing during mixing.
6. the application of the described fermentation liquor composition of claim 1 in the preparation biological organic fertilizer is to add described fermentation liquor composition in the fertilizer that the nature of banking up becomes thoroughly decomposed, and addition is to add 150~250mL fermentation liquor composition, mixing in every 1000g fertilizer.
7. application as claimed in claim 6 is characterized in that described fertilizer is become thoroughly decomposed and formed by the dry branches and fallen leaves organic waste materials of the collecting nature of banking up.
CN2009100366182A 2009-01-13 2009-01-13 Fermentation liquor composition for preparing biological organic fertilizer and preparation and use thereof Expired - Fee Related CN101463334B (en)

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CN102225877B (en) * 2011-04-06 2014-05-21 上海农安生物科技发展有限公司 Alginate composite microbial agent and preparation method thereof
CN102757896A (en) * 2012-07-10 2012-10-31 徐州奇缘生态农业发展有限公司 Efficient microbial soil remediation agent
CN104230582A (en) * 2014-09-11 2014-12-24 红云红河烟草(集团)有限责任公司 Bio-organic fertilizer containing bacillus pumilus
CN105175162B (en) * 2015-10-22 2019-01-11 仲恺农业工程学院 It is a kind of for preventing and treating the microbial organic fertilizer and its preparation and application of bacterial wilt
CN105294181B (en) * 2015-11-30 2018-11-27 江苏纳克生物工程有限公司 The production method of stalk fertilizer rich in colloid bacillus cereus
WO2017186543A2 (en) * 2016-04-24 2017-11-02 Bayer Cropscience Aktiengesellschaft Use of fluopyram and/or bacillus subtilis for controlling fusarium wilt in plants of the musaceae family
CN106083460A (en) * 2016-06-13 2016-11-09 镇江贝思特有机活性肥料有限公司 A kind of bio-feritlizer preventing and treating watermelon blight
CN106172506A (en) * 2016-07-03 2016-12-07 安建慧 A kind of prevention or the method for the treatment of banana Panama disease
CN107739726B (en) * 2017-11-15 2021-03-09 浙江科技学院 Bacillus subtilis and application thereof
CN112088902B (en) * 2020-07-17 2021-08-24 山东元泰生物工程有限公司 Preparation method of plant inducer

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