CN102199049B - Microbial organic manure and preparation method thereof - Google Patents
Microbial organic manure and preparation method thereof Download PDFInfo
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- CN102199049B CN102199049B CN2010105885338A CN201010588533A CN102199049B CN 102199049 B CN102199049 B CN 102199049B CN 2010105885338 A CN2010105885338 A CN 2010105885338A CN 201010588533 A CN201010588533 A CN 201010588533A CN 102199049 B CN102199049 B CN 102199049B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to an agricultural organic fertilizer, and specifically relates to a microbial organic manure and preparation method thereof. The microbial organic manure comprises a microbial organic fertilizer and an absorption microbial fertilizer. Wherein, the proportion of the absorption microbial fertilizer to the microbial organic fertilizer is 5%-10% by mass. According to the invention, microbes are utilized to accelerate the decomposition of raw materials and kill harmful pathogenic bacteria in livestock and poultry dungs. When the manure of the invention is spread into soil, the bacterial strains in the manure reproduce rapidly so that the nutrient potential of the environment can be brought into full play. In addition, lots of fresh humus generated in fermentation can improve the soil, improve fertilizer conservation and supply capability as well as ventilation and water permeability of the soil, stimulate plant growth, and strengthen plant nutrient supply and disease resistance of crops.
Description
Technical field
The present invention relates to agriculture fertilizer, specifically a kind of microbial organic manure and preparation method thereof.
Background technology
At present, agriculture production is because the life-time service inorganic fertilizer, chemical fertilizer utilization ratio is reduced, the nitrogenous fertilizer of 65%-75% is wasted, enter the river with underground water and cause environmental pollution, the phenomenon that hardens appears in soil, the preserve moisture and fertility ability descends, Soil Microorganism descends, and the eubiosis is destroyed, and crop yield reduces, the enrichment of poisonous element in the plant, quality descends, and the agricultural chemicals that uses causes residual and contaminate environment, and if fowl and animal excrement is dealt with another factor that becomes environmental pollution improperly, in recent years along with the raising of people to agricultural product security and quality requirements, the enhancing of environmental consciousness, Development of Organic Agriculture is set up the trend that green food safety has become our agricultural development.And the effect that bio-feritlizer in recent years has is single, and the large microorganism of organism ratio that has is few, DeGrain, even have some to contain inorganic fertilizer, not green fertilizer.
Summary of the invention
The object of the present invention is to provide a kind of microbial organic manure and preparation method thereof.
For achieving the above object, the technical solution used in the present invention
A kind of microbial organic manure: be comprised of microbial organic fertilizer and absorption microbial-bacterial fertilizer, the weight of described absorption microbial-bacterial fertilizer is the 5%-10% of microbial organic fertilizer quality;
Described microbial organic fertilizer is: after 3: 2 by volume ratios of a bacterium liquid and No. two bacterium liquid are mixed, be inoculated in the water content fermentation 7d-10d that banks up in the mixture of the fowl and animal excrement of 40%-60% and straw powder take the inoculum size of 0.1-0.5%, then 60-80 ℃ of lower oven dry, make the fermented product water content of banking up at 30%-40%; A described bacterium liquid is yeast and bacillus subtilis bacteria culture fluid; No. two bacterium liquid are the living actinomycetes nutrient solution of detritus;
Described absorption microbial-bacterial fertilizer is: after No. three bacterium liquid and No. four bacterium liquid were mixed with 2: 3 ratios of volume ratio, with the fermentation of powdered rice hulls whip attachment, wherein bacterium liquid accounted for the 0.1-0.5% of the weight of powdered rice hulls, makes the fermented product water content reach 30%-40%; No. three bacterium liquid are the vinelandii nutrient solution, and No. four bacterium liquid are the gel-shaped bacillus, the nutrient solution of bacillus megaterium and Paenibacillus polymyxa.
A described bacterium liquid is yeast and bacillus subtilis bacteria culture fluid, be specially S. cervisiae (
Saccharomyces cerevisiae), subtilis (
Bacillus subtilis), combined inoculation in 1: 1 by volume is in substratum, and 30 ℃ of anaerobism are cultivated 48-72h.Wherein substratum is, by weight, and W-Gum 20.0 ‰, yeast extract paste 15.0 ‰, dregs of beans 20.0 ‰, MgSO
45.0 ‰, surplus is 1000ml distilled water, pH 6.5-7.0;
Described No. two bacterium liquid are the actinomycetes nutrient solution, be specially the living actinomycetes of detritus (
Actinomyces humiferus) cultivate 48-60h in substratum 25-40 ℃ of aerobic, wherein substratum is, by weight, and 60 ‰ flour, surplus is 1000ml distilled water pH 7.0;
No. three bacterium liquid is the vinelandii nutrient solution, be specially azotobacter vinelandii (
Azotobacter vinelandii) cultivate 48-72h in substratum 25-40 ℃ of aerobic, wherein substratum is, by weight, KH
2PO
40.2 ‰, MgSO
47H
2O 0.2 ‰, and NaCl 0.2 ‰, CaCO
35.0 ‰, C
6H
14O
610.0 ‰, CaSO
42H
2O 0.1 ‰, and surplus is 1000ml distilled water, pH 6.8-7.0;
No. four bacterium liquid are the gel-shaped bacillus, the nutrient solution of bacillus megaterium and Paenibacillus polymyxa.Be specially: the gel-shaped bacillus (
Bacillus mucilaginosus), bacillus megaterium (
Bacillus megaterium) and Paenibacillus polymyxa (
Paenibacillus polymyxa) being by volume 1: 1:1 is mixed in substratum 25-30 ℃ of aerobic environment fermentation 20-40h, and wherein substratum is, by weight, and peptone 5.0 ‰, extractum carnis 3.0 ‰, soil extract 250 ml, surplus is distilled water 750 ml, pH 7.0.
Described soil extract is to get to plough native 400g adding 1000mL distilled water, boils the filtrate that 1h filters gained.
The preparation method of microbial organic manure: with the absorption microbial-bacterial fertilizer mixing and stirring of mentioned microorganism fertilizer and quality 5%-10% thereof, pulverized the 20-40 mesh sieve, and dried and make its water content at 5%-20%.
Described microbial organic fertilizer is: after 3: 2 by volume ratios of a bacterium liquid and No. two bacterium liquid are mixed, be inoculated in the water content fermentation 7d-10d that banks up in the mixture of the fowl and animal excrement of 40%-60% and straw powder take the inoculum size of 0.1-0.5%, then 60-80 ℃ of lower oven dry, make the fermented product water content of banking up at 30%-40%; A described bacterium liquid is yeast and bacillus subtilis bacteria culture fluid; No. two bacterium liquid are the living actinomycetes nutrient solution of detritus;
Described absorption microbial-bacterial fertilizer is: after No. three bacterium liquid and No. four bacterium liquid were mixed with 2: 3 ratios of volume ratio, with the fermentation of powdered rice hulls whip attachment, wherein bacterium liquid accounted for the 0.1-0.5% of the weight of powdered rice hulls, makes the fermented product water content reach 30%-40%; No. three bacterium liquid are the vinelandii nutrient solution, and No. four bacterium liquid are the gel-shaped bacillus, the nutrient solution of bacillus megaterium and Paenibacillus polymyxa.
A described bacterium liquid is yeast and bacillus subtilis bacteria culture fluid, be specially S. cervisiae (
Saccharomyces cerevisiae), subtilis (
Bacillus subtilis), combined inoculation in 1: 1 by volume is in substratum, and 30 ℃ of anaerobism are cultivated 48-72h.Wherein substratum is, by weight, and W-Gum 20.0 ‰, yeast extract paste 15.0 ‰, dregs of beans 20.0 ‰, MgSO
45.0 ‰, surplus is 1000ml distilled water, pH 6.5-7.0;
Described No. two bacterium liquid are the actinomycetes nutrient solution, be specially the living actinomycetes of detritus (
Actinomyces humiferus) cultivate 48-60h in substratum 25-40 ℃ of aerobic, wherein substratum is, by weight, and 60 ‰ flour, surplus is 1000ml distilled water pH 7.0;
No. three bacterium liquid is the vinelandii nutrient solution, be specially azotobacter vinelandii (
Azotobacter vinelandii) cultivate 48-72h in substratum 25-40 ℃ of aerobic, wherein substratum is, by weight, KH
2PO
40.2 ‰, MgSO
47H
2O 0.2 ‰, and NaCl 0.2 ‰, CaCO
35.0 ‰, C
6H
14O
610.0 ‰, CaSO
42H
2O 0.1 ‰, and surplus is 1000ml distilled water, pH 6.8-7.0;
No. four bacterium liquid are the gel-shaped bacillus, the nutrient solution of bacillus megaterium and Paenibacillus polymyxa.Be specially: the gel-shaped bacillus (
Bacillus mucilaginosus), bacillus megaterium (
Bacillus megaterium) and Paenibacillus polymyxa (
Paenibacillus polymyxa) being by volume 1: 1:1 is mixed in substratum 25-30 ℃ of aerobic environment fermentation 20-40h, and wherein substratum is, by weight, and peptone 5.0 ‰, extractum carnis 3.0 ‰, soil extract 250 ml, surplus is distilled water 750 ml, pH 7.0.
Described soil extract is to get to plough native 400g adding 1000mL distilled water, boils the filtrate that 1h filters gained.
All available from Chinese agriculture microbial strains preservation center, its numbering is respectively S. cervisiae and is numbered ACCC20040 described bacterial strain; Subtilis is numbered CICC20037; The living actinomycetes of detritus are numbered ACCC40449; Azotobacter vinelandii is numbered ACCC10087; The gel-shaped bacillus is numbered ACCC10013; Bacillus megaterium is numbered ACCC02991; Paenibacillus polymyxa CICC20185.
The advantage that the present invention has:
Exist yeast to help the fermentation of fowl and animal excrement in the fertilizer of the present invention, the hatching that actinomycetes and Bacillus subtilus suppress worm's ovum has strong restraining effect to pathogenic bacterium, and other mushroom helps the decomposition utilization of Nitrogen In Soils phosphorus potassium.
The present invention utilizes microorganism to accelerate becoming thoroughly decomposed of raw material, and kills the harmful germ in the feces of livestock and poultry.Behind incorporation of fertilizerin the soil of the present invention, bacterial strain Fast-propagation by wherein so that the nutrient potentiality of environment given full play to, and a large amount of fresh humic mass-energy conditioned soil earth that produces during the fermentation, improve soil fertilizer, fertilizer and venting and water permeable ability, the stimulating plant growth, the deliverability of increase plant nutrient strengthens the resistance against diseases of crop.
Attached body explanation
The process flow sheet that Fig. 1 provides for the embodiment of the invention.
Embodiment
Embodiment 1
1) spawn culture: bacterium liquid yeast and bacillus subtilis bacteria culture fluid be S. cervisiae (
Saccharomyces cerevisiae), subtilis (
Bacillus subtilis), combined inoculation in 1: 1 by volume is in substratum, and 30 ℃ of anaerobism are cultivated 48h, and wherein substratum is, by weight, W-Gum 20.0 ‰, yeast extract paste 15.0 ‰, dregs of beans 20.0 ‰, MgSO
45.0 ‰, surplus is 1000ml distilled water, pH 6.5-7.0;
No. two bacterium actinomycetes nutrient solutions be the living actinomycetes of detritus (
Actinomyces humiferus) cultivate 60h in substratum 25-40 ℃ of aerobic, wherein substratum is, by weight, and 60 ‰ flour, surplus is 1000ml distilled water pH 7.0;
No. three liquid-solid nitrogen bacteria culture fluids of bacterium be azotobacter vinelandii (
Azotobacter vinelandii) cultivate 48h in substratum 25-40 ℃ of aerobic, wherein substratum is, by weight, KH
2PO
40.2 ‰, MgSO
47H
2O 0.2 ‰, and NaCl 0.2 ‰, CaCO
35.0 ‰, C
6H
14O
610.0 ‰, CaSO
42H
2O 0.1 ‰, and surplus is 1000ml distilled water, pH 6.8-7.0;
No. four bacterium liquid gel-shaped bacilluss (
Bacillus mucilaginosus), bacillus megaterium (
Bacillus megaterium) and Paenibacillus polymyxa (
Paenibacillus polymyxa) nutrient solution be by volume 1: 1:1 is mixed in substratum 25-30 ℃ of aerobic environment fermentation 24h, and wherein substratum is, by weight, peptone 5.0 ‰, extractum carnis 3.0 ‰, soil extract 250 ml, surplus is distilled water 750 ml, pH 7.0.
All available from Chinese agriculture microbial strains preservation center, its numbering is respectively S. cervisiae and is numbered ACCC20040 described bacterial strain; Subtilis is numbered CICC20037; The living actinomycetes of detritus are numbered ACCC40449; Azotobacter vinelandii is numbered ACCC10087; The gel-shaped bacillus is numbered ACCC10013; Bacillus megaterium is numbered ACCC02991; Paenibacillus polymyxa CICC20185.
2) preparation of microbial organic fertilizer: with bacterium liquid with after No. two bacterium liquid mix in 3: 2 ratios, inoculum size take 0.3% is inoculated in the swine excrement and the mixture of corn stalk powder (granularity is less than 20 orders) of water content as 50%, fermentation 7d banks up, then 70 ℃ of lower oven dry, make the fermented product water content of banking up at 30%-40%.
3) absorption microbial-bacterial fertilizer preparation: after No. three bacterium liquid and No. four bacterium liquid were mixed with 2: 3, with the fermentation of powdered rice hulls (granularity is less than 20 orders) whip attachment, bacterium liquid accounted for the 0.1-0.5% of powdered rice hulls weight, makes its water content reach 40%.
4) preparation of microbial organic manure: with the absorption microbial-bacterial fertilizer mixing and stirring of mentioned microorganism fertilizer and quality 10% thereof, pulverized 20 mesh sieves, and dried and make its water content 10%.
Embodiment 2
1) spawn culture: spawn culture: bacterium liquid yeast and bacillus subtilis bacteria culture fluid be S. cervisiae (
Saccharomyces cerevisiae), subtilis (
Bacillus subtilis), in substratum, 30 ℃ of anaerobism were cultivated 48h by combined inoculation in 1: 1, and wherein substratum is, by weight, W-Gum 20.0 ‰, yeast extract paste 15.0 ‰, dregs of beans 20.0 ‰, MgSO
45.0 ‰, surplus is 1000ml distilled water, pH 6.5-7.0;
No. two bacterium actinomycetes nutrient solutions be the living actinomycetes of detritus (
Actinomyces humiferus) cultivate 60h in substratum 25-40 ℃ of aerobic, wherein substratum is, by weight, and 60 ‰ flour, surplus is 1000ml distilled water pH 7.0;
No. three liquid-solid nitrogen bacteria culture fluids of bacterium be azotobacter vinelandii (
Azotobacter vinelandii) cultivate 48h in substratum 25-40 ℃ of aerobic, wherein substratum is, by weight, KH
2PO
40.2 ‰, MgSO
47H
2O 0.2 ‰, and NaCl 0.2 ‰, CaCO
35.0 ‰, C
6H
14O
610.0 ‰, CaSO
42H
2O 0.1 ‰, and surplus is 1000ml distilled water, and pH 6.8 ~ 7.0;
No. four bacterium liquid gel-shaped bacilluss (
Bacillus mucilaginosus), bacillus megaterium (
Bacillus megaterium) and Paenibacillus polymyxa (
Paenibacillus polymyxa) nutrient solution for by 1: 1:1 is mixed in the substratum 25-30 ℃ of aerobic environment 24h that ferments, and wherein substratum is, by weight, peptone 5.0 ‰, extractum carnis 3.0 ‰, soil extract 250 ml, surplus is distilled water 750 ml, pH 7.0.
2) preparation of microbial organic fertilizer: with bacterium liquid with after No. two bacterium liquid mix in 3: 2 ratios, inoculum size take 0.5% is inoculated in the broiler chicken ight soil and the mixture of corn stalk powder of water content as 50%, fermentation 7d banks up, then 70 ℃ of lower oven dry, make the fermented product water content of banking up at 30%-40%.
3) absorption microbial-bacterial fertilizer preparation: after No. three bacterium liquid and No. four bacterium liquid were mixed with 2: 3, with the fermentation of powdered rice hulls (granularity is less than 20 orders) whip attachment, bacterium liquid accounted for the 0.1-0.5% of powdered rice hulls weight, makes its water content reach 40%.
4) preparation of microbial organic manure: with the absorption microbial-bacterial fertilizer mixing and stirring of mentioned microorganism fertilizer and quality 5% thereof, pulverized 20 mesh sieves, and dried and make its water content 10%.
Embodiment 3
Take greenhouse tomato as test materials, design two treatment group, use the test group of control group and the use microbial organic fertilizer of common compost, two kinds of fertilizer all use as base manure, and usage quantity is 3000kg/ mu.Each test treatment group is established 4 repetitions, and each repeats 100m
2, Routine Management.The observation microbial organic fertilizer sees table 1 for details to the impact of tomato yield.
Table 1 is used microbial organic fertilizer to the impact of tomato yield
| Process | Single fruit weight (g) | Individual plant heavy (kg) | Output (kg/ mu) |
| Contrast | 101.3 | 2.19 | 6118.7 |
| Microbial organic fertilizer | 113.8 | 2.47 | 6794.1 |
Claims (7)
1. microbial organic manure is characterized in that: be comprised of microbial organic fertilizer and absorption microbial-bacterial fertilizer, the weight of described absorption microbial-bacterial fertilizer is the 5%-10% of microbial organic fertilizer quality;
Described microbial organic fertilizer is: after 3: 2 by volume ratios of a bacterium liquid and No. two bacterium liquid are mixed, be inoculated in the water content fermentation 7d-10d that banks up in the mixture of the fowl and animal excrement of 40%-60% and straw powder take the inoculum size of 0.1-0.5%, then 60-80 ℃ of lower oven dry, make the fermented product water content of banking up at 30%-40%; A described bacterium liquid is yeast and bacillus subtilis bacteria culture fluid; No. two bacterium liquid are the living actinomycetes nutrient solution of detritus;
Described absorption microbial-bacterial fertilizer is: after No. three bacterium liquid and No. four bacterium liquid were mixed with 2: 3 ratios of volume ratio, with the fermentation of powdered rice hulls whip attachment, wherein bacterium liquid accounted for the 0.1-0.5% of the weight of powdered rice hulls, makes the fermented product water content reach 30%-40%; No. three bacterium liquid are the vinelandii nutrient solution, and No. four bacterium liquid are the gel-shaped bacillus, the nutrient solution of bacillus megaterium and Paenibacillus polymyxa.
2. by microbial organic manure claimed in claim 1, it is characterized in that:
A described bacterium liquid is yeast and bacillus subtilis bacteria culture fluid, be specially S. cervisiae (saccharomyces cerevisiae), subtilis (Bacillus subtilis), combined inoculation in 1: 1 by volume is in substratum, and 30 ℃ of anaerobism are cultivated 48-72h;
Wherein substratum is, by weight, and W-Gum 20.0 ‰, yeast extract paste 15.0 ‰, dregs of beans 20.0 ‰, MgSO
45.0 ‰, surplus is 1000ml distilled water, pH 6.5-7.0;
Described No. two bacterium liquid are the actinomycetes nutrient solution, be specially the living actinomycetes of detritus (Actinomyces humiferus) and cultivate 48-60h in substratum 25-40 ℃ of aerobic, wherein substratum is, by weight, 60 ‰ flour, surplus are 1000ml distilled water pH7.0;
No. three bacterium liquid is the vinelandii nutrient solution, is specially azotobacter vinelandii (Azotobacter vinelandii) and cultivates 48-72h in substratum 25-40 ℃ of aerobic, and wherein substratum is, by weight, and KH
2PO
40.2 ‰, MgSO
47H
2O 0.2 ‰, and NaCl 0.2 ‰, CaCO
35.0 ‰, C
6H
14O
610.0 ‰, CaSO
42H
2O 0.1 ‰, and surplus is 1000ml distilled water, pH 6.8-7.0;
No. four bacterium liquid are the gel-shaped bacillus, the nutrient solution of bacillus megaterium and Paenibacillus polymyxa;
Be specially: gel-shaped bacillus (Bacillus mucilaginosus), bacillus megaterium (Bacillus megaterium) and Paenibacillus polymyxa (Paenibacillus polymyxa) are for being mixed in substratum 25-30 ℃ of aerobic environment fermentation 20-40h in 1: 1: 1 by volume, wherein substratum is, by weight, peptone 5.0 ‰, extractum carnis 3.0 ‰, soil extract 250ml, surplus is distilled water 750ml, pH 7.0.
3. by microbial organic manure claimed in claim 2, it is characterized in that: described soil extract is to get to plough native 400g adding 1000ml distilled water, boils the filtrate that 1h filters gained.
4. the preparation method of a microbial organic manure claimed in claim 1, it is characterized in that: with the absorption microbial-bacterial fertilizer mixing and stirring of mentioned microorganism fertilizer and quality 5%-10% thereof, pulverized the 20-40 mesh sieve, and dried and make its water content at 5%-20%.
5. by the preparation method of microbial organic manure claimed in claim 4, it is characterized in that:
Described microbial organic fertilizer is: after 3: 2 by volume ratios of a bacterium liquid and No. two bacterium liquid are mixed, be inoculated in the water content fermentation 7d-10d that banks up in the mixture of the fowl and animal excrement of 40%-60% and straw powder take the inoculum size of 0.1-0.5%, then 60-80 ℃ of lower oven dry, make the fermented product water content of banking up at 30%-40%; A described bacterium liquid is yeast and bacillus subtilis bacteria culture fluid; No. two bacterium liquid are the living actinomycetes nutrient solution of detritus;
Described absorption microbial-bacterial fertilizer is: after No. three bacterium liquid and No. four bacterium liquid were mixed with 2: 3 ratios of volume ratio, with the fermentation of powdered rice hulls whip attachment, wherein bacterium liquid accounted for the 0.1-0.5% of the weight of powdered rice hulls, makes the fermented product water content reach 30%-40%; No. three bacterium liquid are the vinelandii nutrient solution, and No. four bacterium liquid are the gel-shaped bacillus, the nutrient solution of bacillus megaterium and Paenibacillus polymyxa.
6. by the preparation method of microbial organic manure claimed in claim 5, it is characterized in that:
A described bacterium liquid is yeast and bacillus subtilis bacteria culture fluid, be specially S. cervisiae (
Saccharomyces cerevisiae), subtilis (
Bacillus subtilis), combined inoculation in 1: 1 by volume is in substratum, and 30 ℃ of anaerobism are cultivated 48-72h;
Wherein substratum is, by weight, and W-Gum 20.0 ‰, yeast extract paste 15.0 ‰, dregs of beans 20.0 ‰, MgSO
45.0 ‰, surplus is 1000ml distilled water, pH 6.5-7.0;
Described No. two bacterium liquid are the actinomycetes nutrient solution, be specially the living actinomycetes of detritus (
Actinomyces humiferus) cultivate 48-60h in substratum 25-40 ℃ of aerobic, wherein substratum is, by weight, and 60 ‰ flour, surplus is 1000ml distilled water pH 7.0;
No. three bacterium liquid is the vinelandii nutrient solution, be specially azotobacter vinelandii (
Azotobacter vinelandii) cultivate 48-72h in substratum 25-40 ℃ of aerobic, wherein substratum is, by weight, KH
2PO
40.2 ‰, MgSO
47H
2O 0.2 ‰, and NaCl 0.2 ‰, CaCO
35.0 ‰, C
6H
14O
610.0 ‰, CaSO
42H
2O 0.1 ‰, and surplus is 1000ml distilled water, pH 6.8-7.0;
No. four bacterium liquid are the gel-shaped bacillus, the nutrient solution of bacillus megaterium and Paenibacillus polymyxa;
Be specially: the gel-shaped bacillus (
Bacillus mucilaginosus), bacillus megaterium (
Bacillus megaterium) and Paenibacillus polymyxa (
Paenibacillus polymyxa) being by volume 1: 1:1 is mixed in substratum 25-30 ℃ of aerobic environment fermentation 20-40h, and wherein substratum is, by weight, and peptone 5.0 ‰, extractum carnis 3.0 ‰, soil extract 250 ml, surplus is distilled water 750 ml, pH 7.0.
7. by the preparation method of microbial organic manure claimed in claim 6, it is characterized in that: described soil extract is to get to plough native 400g adding 1000mL distilled water, boils the filtrate that 1h filters gained.
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| CN102826875B (en) * | 2012-09-12 | 2015-01-28 | 钦州福德培植生物研究所 | Microbial organic fertilizer and purpose of microbial organic fertilizer |
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| CN107473867B (en) * | 2017-10-09 | 2020-12-22 | 山东沃科奇实业有限公司 | Biological bacterial fertilizer prepared from livestock and poultry waste and preparation method thereof |
| CN108329917A (en) * | 2018-04-12 | 2018-07-27 | 李学刚 | A kind of acidic soil conditioner and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1569758A (en) * | 2003-07-26 | 2005-01-26 | 朱绍林 | Organic slow-release fertilizer additive |
| CN101294141A (en) * | 2007-04-28 | 2008-10-29 | 上海四季生物科技有限公司 | A set of living body microorganism preparations for preparing composite microorganism fertilizer and preparation method thereof |
| CN101508601A (en) * | 2009-03-31 | 2009-08-19 | 孙明坤 | Microorganism organic fertilizer and method of preparing the same |
| CN101885625A (en) * | 2010-06-22 | 2010-11-17 | 武汉凯迪工程技术研究总院有限公司 | Solid fermentation method for producing microbial agent by using biomass power plant ash as carrier |
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