CN103483073B - Special compound microorganism bacterial fertilizer for peanuts and production method thereof - Google Patents
Special compound microorganism bacterial fertilizer for peanuts and production method thereof Download PDFInfo
- Publication number
- CN103483073B CN103483073B CN201310417556.6A CN201310417556A CN103483073B CN 103483073 B CN103483073 B CN 103483073B CN 201310417556 A CN201310417556 A CN 201310417556A CN 103483073 B CN103483073 B CN 103483073B
- Authority
- CN
- China
- Prior art keywords
- parts
- bacterium powder
- liquid
- culture
- days
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention belongs to the technical field of fertilizer, particularly relates to special full-dissolubility compound microorganism bacterial fertilizer for peanuts and further relates to a production method of the special fertilizer for the peanuts. The special compound microorganism bacterial fertilizer for the peanuts comprises, by weight, 1-5 parts of compound microorganism bacterial agent, 5-10 parts of furfural residue, 5-25 parts of ammonium sulfate, 5-20 parts of monopotassium phosphate, 5-20 parts of diammonium hydrogen phosphate and 5-20 parts of potassium sulphate. The fertilizer can effectively prevent root rot of the peanuts, can change the micro-ecological environment of soil on the peripheries of root systems of the peanuts, can increase breathability of the soil, eliminate soil hardening and neutralize alkalinity, and can make yield of the peanuts increased by at least 20%.
Description
Technical field
The invention belongs to technical field of fertilizers, be specifically related to a kind of full dissolubility peanut specific complex microbial-bacterial fertilizer, also relate to the production method of this fertilizer specially for peanut.
background technology
Peanut is a kind of important oil crops, plant in massif gravel soil, Plain atteration and southern red yellow earth China is multiple, the problem that these soil ubiquities dysplasia, nutritive ingredient lacks, therefore, the special fertilizer of peanut cultivation should with activating soil, improvement soil microenvironment, the organic fertilizer increasing fertility is main, and chemical fertilizer is auxiliary.At present, during peanut cultivation, ubiquity fertilising kind, the extremely irrational phenomenon of nutrition configuration, ignores the reasonably combined of nutritive element, causes soil nutrient serious unbalance, affect the yield and quality of peanut.In addition in recent years, because the peanut continuous cropping time limit extends, the problems such as peanut disease bacterium resistance, peanut disease is day by day serious, especially the most serious with Roots of Peanut maize ear rot, medication again when common prevention and treatment method is morbidity, but impact is caused on the yield and quality of peanut.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides a kind of generation that effectively can prevent and treat Roots of Peanut maize ear rot, change Peanut Root System surrounding soil micro-ecological environment, increase the ventilation property of soil, eliminate soil compaction, neutralization alkalescence, can make the peanut specific complex microbial-bacterial fertilizer of peanut yield increasing at least 20%, the present invention is by Bacillus licheniformis, bacillus laterosporus and trichoderma harziarum, and acting in conjunction is in soil;
Present invention also offers its production method of above-mentioned bacterial manure, the method to be administered in soil after spawn culture, flora microbial decomposition in fertilizer, the various nutrient of continuous release absorbs for peanut, and in the micro-ecological environment of microorganism species around Peanut Root System, occupy growth vigor, can the generation of effectively preventing Roots of Peanut maize ear rot.
One cultivates peanut specific complex microbial-bacterial fertilizer, and this fertilizer comprises the component of following portions by weight:
Complex microorganism bacteria agent 1 ~ 5 part
Furfural dregs 5 ~ 10 parts
5 ~ 25 parts, ammonium sulfate
Secondary ammonium phosphate 5 ~ 20 parts
Potassium sulfate 5 ~ 20 parts
Potassium primary phosphate 5 ~ 20 parts.
Preferably, above-mentioned fertilizer comprises the component of following parts by weight:
Complex microorganism bacteria agent 2 ~ 4 parts
Furfural dregs 6 ~ 8 parts
10 ~ 20 parts, ammonium sulfate
Secondary ammonium phosphate 10 ~ 16 parts
Potassium sulfate 10 ~ 16 parts
Potassium primary phosphate 10 ~ 16 parts.
Preferred, above-mentioned fertilizer comprises the component of following parts by weight:
Complex microorganism bacteria agent 3 parts
Furfural dregs 8 parts
16 parts, ammonium sulfate
Secondary ammonium phosphate 15 parts
Potassium sulfate 15 parts
Potassium primary phosphate 15 parts.
The preparation process of complex microorganism bacteria agent of the present invention is as follows: by Bacillus licheniformis, bacillus laterosporus and trichoderma harziarum three bacterial classifications individually inoculation fermentation, and the lichen bacillus ferments liquid, bacillus laterosporus fermented liquid are pulverized respectively through oven dry and obtained Bacillus licheniformis powder, bacillus laterosporus powder; Trichoderma harziarum zymophyte block obtains Trichoderma powder through drying in the shade, pulverizing, and finally by three kinds of bacterium powder mixing, obtains complex microorganism bacteria agent.
Above-mentioned composite bacillus microbial inoculum preparation process is specially:
The preparation of bacillus licheniformis agent:
Liquid submerged fermentation is cultivated, Bacillus licheniformis strain is inoculated in disinfection fermentation tank, fermention medium is: starch 0.2-0.8%, yeast extract paste 0.01-0.06%, dregs of beans 0.2-0.8%, urea 0.1-0.4%, dipotassium hydrogen phosphate 0.2-0.8%, potassium primary phosphate 0.1-0.5%, magnesium sulfate 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 0.9-1.1:0.5, culture temperature 35-40 DEG C, agitation revolution 100-200rpm, dissolved oxygen is not less than 30%, pH value is not less than 6.5, stir culture 6-8 hour, viable bacteria content is made to reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder,
Prepared by bacillus laterosporus microbial inoculum: by bacillus laterosporus strain inoculation to disinfection fermentation tank, fermention medium is: starch 0.2-0.8%, yeast extract paste 0.01-0.06%, dregs of beans 0.2-0.8%, urea 0.1-0.4%, dipotassium hydrogen phosphate 0.2-0.8%, potassium primary phosphate 0.1-0.5%, magnesium sulfate 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 0.9-1.1:0.5, culture temperature 35-40 DEG C, agitation revolution 100-200rpm, dissolved oxygen is not less than 30%, pH value is not less than 6.5, stir culture 6-8 hour, viable bacteria content is made to reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder,
Trichoderma harziarum shallow tray fermentation and microbial inoculum preparation: liquid rotating solid biphasic cultivation method is cultivated, conidium liquid is proceeded in PD liquid nutrient medium, shaking culture 2-4 days, then according to the part by weight of 1:6-10 and the rice bran of sterilizing or wheat bran Homogeneous phase mixing, be placed in the stainless steel mesh screen of liming or ozonization, cover the plastics film of sterilization above, after being placed in 22-30 DEG C indoor cultivation 3-5 days, open plastics film, produce spore 3-5 days; Collect together with culture material, the moon dries in the air 5-7 days, puts into after pulverizer pulverizes, crosses 30 mesh sieves and obtain bacterium powder;
Prepared by complex microorganism bacteria agent:
The Bacillus licheniformis powder obtained in above-mentioned steps, bacillus laterosporus bacterium powder, trichoderma harzianum bacterium powder are mixed according to the ratio of 1-4:1-4:1, obtains complex microorganism bacteria agent.
Get furfural dregs 5 ~ 10 parts, 5 ~ 25 parts, ammonium sulfate, Secondary ammonium phosphate 5 ~ 20 parts, potassium sulfate 5 ~ 20 parts, potassium primary phosphate 5 ~ 20 parts, above-mentioned raw material is mixed, dry at 90 DEG C, after feeding crusher in crushing is powdered, be added with complex microorganism bacteria agent 1 ~ 5 part mixing that above-mentioned step prepares, obtain peanut specific complex microbial-bacterial fertilizer.
As a modification of the present invention, in the preparation method of peanut specific complex microbial-bacterial fertilizer, composite bacillus microbial inoculum preparation process is specially:
The preparation of bacillus licheniformis agent:
Liquid submerged fermentation is cultivated, and be inoculated into by Bacillus licheniformis strain in disinfection fermentation tank, fermention medium is: starch 0.5%, yeast extract paste 0.03%, dregs of beans 0.5%, urea 0.2%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.25%, magnesium sulfate 0.1%, manganous sulfate 0.01%, pH7.0, fermentation condition: ventilation ratio is initiated with 1:0.5, culture temperature 37 DEG C, agitation revolution 150rpm, dissolved oxygen is not less than 30%, pH value is not less than 6.5, stir culture 7 hours, makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, bacterium mud at 45 DEG C forced air drying to water content 30%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder;
Prepared by bacillus laterosporus microbial inoculum: by bacillus laterosporus strain inoculation to disinfection fermentation tank, fermention medium is: starch 0.5%, yeast extract paste 0.03%, dregs of beans 0.5%, urea 0.2%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.25%, magnesium sulfate 0.1%, manganous sulfate 0.01%, pH7.0, fermentation condition: ventilation ratio is initiated with 1:0.5, culture temperature 37 DEG C, agitation revolution 150rpm, dissolved oxygen is not less than 30%, and pH value is not less than 6.5, stir culture 7 hours, makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, bacterium mud at 45 DEG C forced air drying to water content 30%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder;
Trichoderma harziarum shallow tray fermentation and microbial inoculum preparation: liquid rotating solid biphasic cultivation method is cultivated, cultural method is liquid rotating solid biphasic cultivation method, conidium liquid is proceeded in PD liquid nutrient medium, shaking culture 3 days, then according to the part by weight of 1:8 and the rice bran of sterilizing or wheat bran Homogeneous phase mixing, is placed in the stainless steel mesh screen of liming or ozonization, cover the plastics film of sterilization above, after 4 days, open plastics film at indoor cultivation at being placed in 28 DEG C, produce spore 4 days; Collect together with culture material, the moon dries in the air 6 days, after putting into pulverizer pulverizing, crosses 30 mesh sieves and obtains bacterium powder;
Prepared by complex micro organism fungicide:
The Bacillus licheniformis powder obtained in above step, bacillus laterosporus bacterium powder, trichoderma harzianum bacterium powder are mixed according to the ratio of 2:2:1, obtains complex microorganism bacteria agent.
In the present invention, the complex micro organism fungicide in base fertilizer adopts the lichen bacillus ferments liquid dry powder, the bacterium powder of bacillus laterosporus fermented liquid dry powder and these three kinds of bacterium of trichoderma harzianum powder mixes.
Beneficial effect after the present invention uses peanut is, fertilizer provided by the present invention effectively can prevent and treat the generation of Roots of Peanut maize ear rot, changes Peanut Root System surrounding soil micro-ecological environment, increases the ventilation property of soil, eliminates soil compaction, neutralization alkalescence, can make peanut yield increasing at least 20%.
The principle producing above beneficial effect is: be administered to after in soil by special for peanut of the present invention bacterial manure, the microbial decomposition of flora in fertilizer, various nutrient can be discharged continuously absorb for peanut, simultaneously, the organic acid produced in microbial decomposition process and mineral acid, can the alkalescence of effective sweetening of the soil, be conducive to the growth of peanut.
In addition, in the micro-ecological environment of microorganism species around Peanut Root System, occupy growth vigor, effectively can prevent and treat the generation of Roots of Peanut maize ear rot.
The innovative point more than invented is:
The selection of bacterial classification: the compound bacterial fertilizer of Bacillus licheniformis, bacillus laterosporus and trichoderma harziarum, compared with existing compound bacterial fertilizer, through field test, obvious promotion offspring growth, effective resistance peanut disease and pest, in raising peanut yield and quality, has obvious advantage.
Microbial-bacterial fertilizer bacterium viable bacteria content reaches 1,000 hundred million/gram, considerably beyond domestic other products, reason is, three of the present invention the adopted strain bacterial classifications zymotechniques that premenstruum, research was established, can obtain stable high viable bacteria content; In microbial inoculum preparation process, strictly control bake out temperature, at utmost ensure high viable bacteria content, viable bacteria content about 20 times can be improved.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described, so that those skilled in the art more understands the present invention, but does not therefore limit the present invention.
Embodiment 1
The preparation of bacillus licheniformis agent:
Liquid submerged fermentation is cultivated, and be inoculated into by Bacillus licheniformis strain in disinfection fermentation tank, fermention medium is: starch 0.5%, yeast extract paste 0.03%, dregs of beans 0.5%, urea 0.2%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.25%, magnesium sulfate 0.1%, manganous sulfate 0.01%, pH7.0, fermentation condition: ventilation ratio is initiated with 1:0.5, culture temperature 37 DEG C, agitation revolution 150rpm, dissolved oxygen is not less than 30%, pH value is not less than 6.5, stir culture 7 hours, makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, and the 45-50 DEG C of forced air drying of bacterium mud is to water content 30%, and cyclonic separation bacterium powder, puts into pulverizer and pulverize, and crosses 30 mesh sieves and obtains bacterium powder;
Prepared by bacillus laterosporus microbial inoculum: by bacillus laterosporus strain inoculation to disinfection fermentation tank, fermention medium is: starch 0.5%, yeast extract paste 0.03%, dregs of beans 0.5%, urea 0.2%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.25%, magnesium sulfate 0.1%, manganous sulfate 0.01%, pH7.0, fermentation condition: ventilation ratio is initiated with 1:0.5, culture temperature 37 DEG C, agitation revolution 150rpm, dissolved oxygen is not less than 30%, and pH value is not less than 6.5, stir culture 7 hours, makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, and the 45 DEG C of forced air dryings of bacterium mud are to water content 30%, and cyclonic separation bacterium powder, puts into pulverizer and pulverize, and crosses 30 mesh sieves and obtains bacterium powder;
Prepared by bacillus laterosporus microbial inoculum: by bacillus laterosporus strain inoculation to disinfection fermentation tank, fermention medium is: starch 0.5%, yeast extract paste 0.03%, dregs of beans 0.5%, urea 0.2%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.25%, magnesium sulfate 0.1%, manganous sulfate 0.01%, pH7.0, fermentation condition: ventilation ratio is initiated with 1:0.5, culture temperature 37 DEG C, agitation revolution 150rpm, dissolved oxygen is not less than 30%, and pH value is not less than 6.5, stir culture 7 hours, makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, bacterium mud at 45 DEG C forced air drying to water content 30%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder;
Trichoderma harziarum shallow tray fermentation and microbial inoculum preparation: liquid rotating solid biphasic cultivation method is cultivated, cultural method is liquid rotating solid biphasic cultivation method, conidium liquid is proceeded in PD liquid nutrient medium, shaking culture 3 days, then according to the part by weight of 1:8 and the rice bran of sterilizing or wheat bran Homogeneous phase mixing, is placed in the stainless steel mesh screen of liming or ozonization, cover the plastics film of sterilization above, after 4 days, open plastics film at indoor cultivation at being placed in 28 DEG C, produce spore 4 days; Collect together with culture material, the moon dries in the air 6 days, after putting into pulverizer pulverizing, crosses 30 mesh sieves and obtains bacterium powder;
Prepared by complex micro organism fungicide:
The Bacillus licheniformis powder obtained in above step, bacillus laterosporus bacterium powder, trichoderma harzianum bacterium powder are mixed according to the ratio of 2:2:1, obtains complex microorganism bacteria agent.
Get furfural dregs 8 parts, 16 parts, ammonium sulfate, Secondary ammonium phosphate 15 parts, potassium sulfate 15 parts, potassium primary phosphate 15 parts, above-mentioned raw material is mixed, dry at 90 DEG C, after feeding crusher in crushing is powdered, be added with the complex microorganism bacteria agent 3 parts mixing that above-mentioned step prepares, obtain peanut specific complex microbial-bacterial fertilizer.
Comparative example 1
Be with the difference of embodiment 1, in comparative example 1, complex microorganism bacteria agent only comprises the combination of bacillus licheniformis agent, these two kinds of microbial inoculums of bacillus laterosporus microbial inoculum, and the add-on of two kinds of bacterium is total up to 3 parts, and its weight ratio is 1:1; The preparation method of two kinds of above-mentioned bacterial classifications is identical with embodiment 1, and remaining step is also identical;
Comparative example 2
Be with the difference of embodiment 1, in comparative example 1, complex microorganism bacteria agent only comprises the combination of bacillus licheniformis agent, these two kinds of microbial inoculums of trichoderma harziarum bacteria agent, and the add-on of two kinds of bacterium is total up to 3 parts, and its weight ratio is 1:1; The preparation method of two kinds of above-mentioned bacterial classifications is identical with embodiment 1, and remaining step is also identical;
Comparative example 3
Be with the difference of embodiment 1, in comparative example 1, complex microorganism bacteria agent only comprises the combination of bacillus laterosporus microbial inoculum, these two kinds of microbial inoculums of trichoderma harziarum bacteria agent, and the add-on of two kinds of bacterium is total up to 3 parts, and its weight ratio is 1:1; The preparation method of two kinds of above-mentioned bacterial classifications is identical with embodiment 1, and remaining step is also identical;
As can be seen from above embodiment 1 and comparative example 1,2,3, the combination of three kinds of bacterial classifications of the present invention, its fertilizer efficiency is good, and data are as follows:
Choose same piece of land, be divided into 4 pieces, use the fertilizer of embodiment 1, comparative example 1,2,3 respectively, its fertilizer efficiency is compared as follows:
Embodiment 1, comparative example 1,2,3 use fertility contrast (unit mg/kg) (available nitrogen) before and after soil
Embodiment 1, comparative example 1,2,3 use fertility contrast (unit mg/kg) (rapid available phosphorus) before and after soil
Embodiment 1, comparative example 1,2,3 use fertility contrast (unit mg/kg) (available potassium) before and after soil
Field experiment
Choose same soil, be divided into 4 parts, the peanut of same kind in sowing, when peanut starts to emerge, the fertilizer of identical weight is used respectively in these 4 pieces of grounds, use embodiment 1, comparative example 1, comparative example 2, comparative example 3 fertilizer after, growth the regular hour after, peanut seedling growth result is as follows:
The effects such as the output after harvesting peanut
As can be seen from above data relatively in, fertilizer of the present invention is appended to after in the soil of plantation peanut, and its fertilizer efficiency to be better than in comparative example 1,2,3 any one group, visible, the combination of these three kinds of bacterial classifications of the present invention is better than the combined effect of any two kinds in above three kinds of bacterial classifications.
In embodiment 1, the sickness rate of Roots of Peanut maize ear rot is 2%;
In comparative example 1, the sickness rate of Roots of Peanut maize ear rot is 11%;
In comparative example 2, the sickness rate of Roots of Peanut maize ear rot is 15%;
In comparative example 3, the sickness rate of Roots of Peanut maize ear rot is 13%;
After using commercially available common chemical fertilizer, the sickness rate of Roots of Peanut maize ear rot is 30%.
Embodiment 2
The preparation of bacillus licheniformis agent:
Liquid submerged fermentation is cultivated, and be inoculated into by Bacillus licheniformis strain in disinfection fermentation tank, fermention medium is: starch 0.2%, yeast extract paste 0.01%, dregs of beans 0.2%, urea 0.1%, dipotassium hydrogen phosphate 0.2%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, manganous sulfate 0.006%, pH6.8, fermentation condition: ventilation ratio is initiated with 0.9, culture temperature 35 DEG C, agitation revolution 100rpm, dissolved oxygen is not less than 30%, pH value is not less than 6.5, stir culture 6 hours, makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, and about the 45 DEG C forced air dryings of bacterium mud are to water content 25%, and cyclonic separation bacterium powder, puts into pulverizer and pulverize, and crosses 30 mesh sieves and obtains bacterium powder;
Prepared by bacillus laterosporus microbial inoculum: by bacillus laterosporus strain inoculation to disinfection fermentation tank, fermention medium is: starch 0.2%, yeast extract paste 0.01%, dregs of beans 0.2%, urea 0.1%, dipotassium hydrogen phosphate 0.2%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, manganous sulfate 0.006%, pH6.8, fermentation condition: ventilation ratio is initiated with 1:0.5, culture temperature 35, agitation revolution 100rpm, dissolved oxygen is not less than 30%, and pH value is not less than 6.5, stir culture 6 hours, makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, and the 45 DEG C of forced air dryings of bacterium mud are to water content 25%, and cyclonic separation bacterium powder, puts into pulverizer and pulverize, and crosses 30 mesh sieves and obtains bacterium powder;
Trichoderma harziarum shallow tray fermentation and microbial inoculum preparation: liquid rotating solid biphasic cultivation method is cultivated, conidium liquid is proceeded in PD liquid nutrient medium, shaking culture 3 days, then according to the part by weight of 1:6 and the rice bran of sterilizing or wheat bran Homogeneous phase mixing, be placed in the stainless steel mesh screen of liming or ozonization, cover the plastics film of sterilization above, be placed in the indoor cultivation of 26 DEG C after 3 days, open plastics film, produce spore 4 days; Collect together with culture material, the moon dries in the air 6 days, after putting into pulverizer pulverizing, crosses 30 mesh sieves and obtains bacterium powder;
Prepared by complex microorganism bacteria agent:
The Bacillus licheniformis powder obtained in above step, bacillus laterosporus bacterium powder, trichoderma harzianum bacterium powder are mixed according to the ratio of 2:2:1, obtains complex microorganism bacteria agent.
Get furfural dregs 5 parts, 5 parts, ammonium sulfate, Secondary ammonium phosphate 5 parts, potassium sulfate 5 parts, potassium primary phosphate 5 parts, above-mentioned raw material is mixed, dry at 90 DEG C, after feeding crusher in crushing is powdered, be added with the complex microorganism bacteria agent 1 part mixing that above-mentioned step prepares, obtain peanut specific complex microbial-bacterial fertilizer.
Embodiment 3
The preparation of bacillus licheniformis agent:
Liquid submerged fermentation is cultivated, and be inoculated into by Bacillus licheniformis strain in disinfection fermentation tank, fermention medium is: starch 0.8%, yeast extract paste 0.06%, dregs of beans 0.8%, urea 0.4%, dipotassium hydrogen phosphate 0.8%, potassium primary phosphate 0.5%, magnesium sulfate 0.15%, manganous sulfate 0.012%, pH7.2, fermentation condition: ventilation ratio is initiated with 1:0.5, culture temperature 40 DEG C, agitation revolution 200rpm, dissolved oxygen is not less than 30%, pH value is not less than 6.5, stir culture 8 hours, makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, and the 50 DEG C of forced air dryings of bacterium mud are to water content 35%, and cyclonic separation bacterium powder, puts into pulverizer and pulverize, and crosses 30 mesh sieves and obtains bacterium powder;
Prepared by bacillus laterosporus microbial inoculum: by bacillus laterosporus strain inoculation to disinfection fermentation tank, fermention medium is: starch 0.8%, yeast extract paste 0.06%, dregs of beans 0.8%, urea 0.1-0.4%, dipotassium hydrogen phosphate 0.8%, potassium primary phosphate 0.5%, magnesium sulfate 0.15%, manganous sulfate 0.012%, pH7.2, fermentation condition: ventilation ratio is initiated with 1:0.5, culture temperature 40 DEG C, agitation revolution 200rpm, dissolved oxygen is not less than 30%, and pH value is not less than 6.5, stir culture 8 hours, makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, and the 50 DEG C of forced air dryings of bacterium mud are to water content 35%, and cyclonic separation bacterium powder, puts into pulverizer and pulverize, and crosses 30 mesh sieves and obtains bacterium powder;
Trichoderma harziarum shallow tray fermentation and microbial inoculum preparation: liquid rotating solid biphasic cultivation method is cultivated, conidium liquid is proceeded in PD liquid nutrient medium, shaking culture 4 days, then according to the part by weight of 1:10 and the rice bran of sterilizing or wheat bran Homogeneous phase mixing, be placed in the stainless steel mesh screen of liming or ozonization, cover the plastics film of sterilization above, be placed in the indoor cultivation of 30 DEG C after 5 days, open plastics film, produce spore 5 days; Collect together with culture material, the moon dries in the air 7 days, after putting into pulverizer pulverizing, crosses 30 mesh sieves and obtains bacterium powder;
Prepared by complex microorganism bacteria agent:
The Bacillus licheniformis powder obtained in above step, bacillus laterosporus bacterium powder, trichoderma harzianum bacterium powder are mixed according to the ratio of 2:2:1, obtains complex microorganism bacteria agent.
Get furfural dregs 10 parts, 25 parts, ammonium sulfate, Secondary ammonium phosphate 20 parts, potassium sulfate 20 parts, potassium primary phosphate 20 parts, above-mentioned raw material is mixed, dry at 90 DEG C, after feeding crusher in crushing is powdered, be added with the complex microorganism bacteria agent 5 parts mixing that above-mentioned step prepares, obtain peanut specific complex microbial-bacterial fertilizer.
Embodiment 4
Be with the difference of embodiment 1, in the present embodiment, also add some other the raw material having fertilizer efficiency, specific as follows: in the present embodiment, get furfural dregs 8 parts, 16 parts, ammonium sulfate, Secondary ammonium phosphate 15 parts, potassium sulfate 15 parts, potassium primary phosphate 15 parts, 10 parts, urea, peat composed of rotten mosses ash 10 parts, active sludge 15 parts, 5 parts, straw powder, farm manure 15 parts, above-mentioned raw material is mixed, dry at 90 DEG C, after feeding crusher in crushing is powdered, be added with the complex microorganism bacteria agent 3 parts mixing that above-mentioned step prepares, obtain peanut specific complex microbial-bacterial fertilizer.
Embodiment 5
Be with the difference of embodiment 1, in the present embodiment, add some other auxiliary microbial growths and the raw material with auxiliary disease-resistant effect, specific as follows: in the present embodiment, get furfural dregs 9 parts, 15 parts, ammonium sulfate, Secondary ammonium phosphate 15 parts, potassium sulfate 15 parts, potassium primary phosphate 15 parts, 10 parts, urea, dregs of beans 12 parts, active sludge 14 parts, chitin 5 parts, farm manure 20 parts, above-mentioned raw material is mixed, dry at 90 DEG C, after feeding crusher in crushing is powdered, be added with the complex microorganism bacteria agent 3 parts mixing that above-mentioned step prepares, obtain peanut specific complex microbial-bacterial fertilizer.
Claims (2)
1. a preparation method cultivating peanut specific complex microbial-bacterial fertilizer, comprises following step:
Get furfural dregs 5 ~ 10 parts, 5 ~ 25 parts, ammonium sulfate, potassium primary phosphate 5 ~ 20 parts, Secondary ammonium phosphate 5 ~ 20 parts, potassium sulfate 5 ~ 20 parts, above-mentioned raw material is mixed, dry at 90 DEG C, after feeding crusher in crushing is powdered, add complex micro organism fungicide 1 ~ 5 part mixing, obtain peanut specific complex microbial-bacterial fertilizer;
Its preparation process of described complex micro organism fungicide is specially:
The preparation of bacillus licheniformis agent:
Liquid submerged fermentation is cultivated, Bacillus licheniformis strain is inoculated in disinfection fermentation tank, fermention medium is: starch 0.2-0.8%, yeast extract paste 0.01-0.06%, dregs of beans 0.2-0.8%, urea 0.1-0.4%, dipotassium hydrogen phosphate 0.2-0.8%, potassium primary phosphate 0.1-0.5%, magnesium sulfate 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 0.9-1.1:0.5, culture temperature 35-40 DEG C, agitation revolution 100-200rpm, dissolved oxygen is not less than 30%, pH value is not less than 6.5, stir culture 6-8 hour, viable bacteria content is made to reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder,
Prepared by bacillus laterosporus microbial inoculum: by bacillus laterosporus strain inoculation to disinfection fermentation tank, fermention medium is: starch 0.2-0.8%, yeast extract paste 0.01-0.06%, dregs of beans 0.2-0.8%, urea 0.1-0.4%, dipotassium hydrogen phosphate 0.2-0.8%, potassium primary phosphate 0.1-0.5%, magnesium sulfate 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 0.9-1.1:0.5, culture temperature 35-40 DEG C, agitation revolution 100-200rpm, dissolved oxygen is not less than 30%, pH value is not less than 6.5, stir culture 6-8 hour, viable bacteria content is made to reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder,
Trichoderma harziarum shallow tray fermentation and microbial inoculum preparation: liquid rotating solid biphasic cultivation method is cultivated, conidium liquid is proceeded in PD liquid nutrient medium, shaking culture 2-4 days, then according to the part by weight of 1:6-10 and the rice bran of sterilizing or wheat bran Homogeneous phase mixing, be placed in the stainless steel mesh screen of liming or ozonization, cover the plastics film of sterilization above, after being placed in 22-30 DEG C indoor cultivation 3-5 days, open plastics film, produce spore 3-5 days; Collect together with culture material, the moon dries in the air 5-7 days, puts into after pulverizer pulverizes, crosses 30 mesh sieves and obtain bacterium powder;
The Bacillus licheniformis powder obtained in above-mentioned steps, bacillus laterosporus bacterium powder, trichoderma harzianum bacterium powder are mixed according to the ratio of 1-4:1-4:1, obtains complex micro organism fungicide.
2. as claimed in claim 1 one preparation method cultivating peanut specific complex microbial-bacterial fertilizer, is characterized in that, in the preparation process of complex micro organism fungicide,
The preparation of bacillus licheniformis agent:
Liquid submerged fermentation is cultivated, and be inoculated into by Bacillus licheniformis strain in disinfection fermentation tank, fermention medium is: starch 0.5%, yeast extract paste 0.03%, dregs of beans 0.5%, urea 0.2%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.25%, magnesium sulfate 0.1%, manganous sulfate 0.01%, pH7.0, fermentation condition: ventilation ratio is initiated with 1:0.5, culture temperature 37 DEG C, agitation revolution 150rpm, dissolved oxygen is not less than 30%, pH value is not less than 6.5, stir culture 7 hours, makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, bacterium mud at 45 DEG C forced air drying to water content 30%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder;
Prepared by bacillus laterosporus microbial inoculum: by bacillus laterosporus strain inoculation to disinfection fermentation tank, fermention medium is: starch 0.5%, yeast extract paste 0.03%, dregs of beans 0.5%, urea 0.2%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.25%, magnesium sulfate 0.1%, manganous sulfate 0.01%, pH7.0, fermentation condition: ventilation ratio is initiated with 1:0.5, culture temperature 37 DEG C, agitation revolution 150rpm, dissolved oxygen is not less than 30%, and pH value is not less than 6.5, stir culture 7 hours, makes viable bacteria content reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, bacterium mud at 45 DEG C forced air drying to water content 30%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder;
Trichoderma harziarum shallow tray fermentation and microbial inoculum preparation: liquid rotating solid biphasic cultivation method is cultivated, cultural method is liquid rotating solid biphasic cultivation method, conidium liquid is proceeded in PD liquid nutrient medium, shaking culture 3 days, then according to the part by weight of 1:8 and the rice bran of sterilizing or wheat bran Homogeneous phase mixing, is placed in the stainless steel mesh screen of liming or ozonization, cover the plastics film of sterilization above, after 4 days, open plastics film at indoor cultivation at being placed in 28 DEG C, produce spore 4 days; Collect together with culture material, the moon dries in the air 6 days, after putting into pulverizer pulverizing, crosses 30 mesh sieves and obtains bacterium powder;
The Bacillus licheniformis powder obtained in above step, bacillus laterosporus bacterium powder, trichoderma harzianum bacterium powder are mixed according to the ratio of 2:2:1, obtains complex microorganism bacteria agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310417556.6A CN103483073B (en) | 2013-09-14 | 2013-09-14 | Special compound microorganism bacterial fertilizer for peanuts and production method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310417556.6A CN103483073B (en) | 2013-09-14 | 2013-09-14 | Special compound microorganism bacterial fertilizer for peanuts and production method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103483073A CN103483073A (en) | 2014-01-01 |
| CN103483073B true CN103483073B (en) | 2015-01-07 |
Family
ID=49823736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310417556.6A Active CN103483073B (en) | 2013-09-14 | 2013-09-14 | Special compound microorganism bacterial fertilizer for peanuts and production method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103483073B (en) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103848702A (en) * | 2014-01-14 | 2014-06-11 | 淅川县绿新生物科技有限公司 | Fertilizer for peanuts |
| CN104396486B (en) * | 2014-10-27 | 2016-06-22 | 山东省农作物种质资源中心 | A kind of implantation methods of winter wheat |
| CN105237110A (en) * | 2015-08-27 | 2016-01-13 | 青岛深蓝肥业有限公司 | Anti-disease composite microbial fertilizer specially-used for American ginseng and preparation method thereof |
| CN105347952A (en) * | 2015-12-02 | 2016-02-24 | 合肥四方磷复肥有限责任公司 | Naturally-flocculent microbial fertilizer and preparation method thereof |
| CN105948992A (en) * | 2016-05-14 | 2016-09-21 | 安徽新天地生物肥业有限公司 | Special organic slow release fertilizer for peanuts |
| CN106342858A (en) * | 2016-08-26 | 2017-01-25 | 安徽久易农业股份有限公司 | Post-emergence compound herbicide for wheat fields |
| CN106748036A (en) * | 2016-12-04 | 2017-05-31 | 钦州市星火计划办公室 | One cultivates peanut composite microbic bacterial fertilizer and preparation method thereof |
| CN107382637A (en) * | 2017-09-26 | 2017-11-24 | 广西进旺盛农业高新科技开发有限公司 | A kind of composite microbic bacterial fertilizer |
| CN110483206A (en) * | 2018-08-29 | 2019-11-22 | 郑州海绿王绿色科技有限公司 | A kind of anti-continuous cropping complex micro organism fungicide |
| CN112457138A (en) * | 2020-12-21 | 2021-03-09 | 东北农业大学 | Microbial preparation for improving soil fertility of saline-alkali soil and preparation method thereof |
| CN112679256A (en) * | 2021-01-13 | 2021-04-20 | 上海市环境科学研究院 | Preparation method of straw biogas residue-based composite microbial fertilizer |
| CN113303316B (en) * | 2021-06-16 | 2023-02-17 | 茂生生物科技保定有限公司 | High-efficiency sterilization and disinfection method for seedbed soil |
| CN114394863A (en) * | 2022-01-18 | 2022-04-26 | 山东省农业科学院 | Microbial water-soluble fertilizer for preventing and treating peanut diseases and insect pests and preparation method thereof |
| CN115259963A (en) * | 2022-06-23 | 2022-11-01 | 中盐安徽红四方肥业股份有限公司 | Organic-inorganic compound fertilizer containing functional bacteria and production method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2252553A (en) * | 1991-01-07 | 1992-08-12 | E J Godwin | Plant growth medium |
| CN101993299A (en) * | 2009-08-31 | 2011-03-30 | 夏荣堂 | Special microbial foliar fertilizer for peanuts |
| CN102001870A (en) * | 2010-11-11 | 2011-04-06 | 唐清池 | Inorganic-organic microbial compound fertilizer and preparation method thereof |
| CN102002470A (en) * | 2010-11-11 | 2011-04-06 | 唐清池 | Quick-acting multi-microorganism composite water flush fertilizer and preparation method thereof |
| CN102816005A (en) * | 2012-09-10 | 2012-12-12 | 临沂中磷生物科技有限公司 | Peanut special microbial fertilizer and production method thereof |
-
2013
- 2013-09-14 CN CN201310417556.6A patent/CN103483073B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2252553A (en) * | 1991-01-07 | 1992-08-12 | E J Godwin | Plant growth medium |
| CN101993299A (en) * | 2009-08-31 | 2011-03-30 | 夏荣堂 | Special microbial foliar fertilizer for peanuts |
| CN102001870A (en) * | 2010-11-11 | 2011-04-06 | 唐清池 | Inorganic-organic microbial compound fertilizer and preparation method thereof |
| CN102002470A (en) * | 2010-11-11 | 2011-04-06 | 唐清池 | Quick-acting multi-microorganism composite water flush fertilizer and preparation method thereof |
| CN102816005A (en) * | 2012-09-10 | 2012-12-12 | 临沂中磷生物科技有限公司 | Peanut special microbial fertilizer and production method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103483073A (en) | 2014-01-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103483073B (en) | Special compound microorganism bacterial fertilizer for peanuts and production method thereof | |
| CN104003817B (en) | Special disease-resistant compound microbial bacterial manure of a kind of corn and preparation method thereof | |
| CN104177137B (en) | A kind of microbial straw composing agent and preparation method thereof | |
| CN104016807B (en) | Special organic microbial fertilizer of a kind of garlic and preparation method thereof | |
| CN102199049B (en) | Microbial organic manure and preparation method thereof | |
| CN101941851B (en) | Technology and process for preparing biochemical humic acid by using kitchen waste | |
| CN103966150B (en) | A kind of agricultural crop straw decomposing microbial inoculum and using method thereof and effect | |
| CN102517217B (en) | Environment-friendly straw fermenting agent | |
| CN102010241B (en) | Microbial ferment organic fertilizer and preparation method thereof | |
| CN105237110A (en) | Anti-disease composite microbial fertilizer specially-used for American ginseng and preparation method thereof | |
| CN103966148B (en) | A kind of vegetable castoff decomposing microbial inoculum and using method thereof and made organic substrate | |
| CN106748341A (en) | Potato microbial bacterial manure special and preparation method thereof | |
| CN102442849A (en) | Disease-preventing bio-organic fertilizer prepared from straw and its preparation method | |
| CN108017445A (en) | A kind of reclamation of salinep-alkali soil type microbial bacterial agent and preparation method thereof | |
| CN102337237B (en) | Functional organic material decomposing agent and preparation method thereof | |
| CN104926530A (en) | High-efficiency biological organic fertilizer for organic crops and preparation method thereof | |
| CN103819267A (en) | Bacterial manure for peanut planting and preparation method of bacterial manure | |
| CN103194405B (en) | Growth-promoting bacteria for promoting ginger growth and preventing and controlling continuous cropping ginger soil-borne wilt and microorganism organic fertilizer produced from growth-promoting bacteria | |
| CN102199564A (en) | Composite microbial agent for direct decomposition of crop straws returned to field and preparation method thereof | |
| CN104099281A (en) | Environment-friendly compound microbial agent | |
| CN105110921A (en) | Microbial fertilizer for accelerating crop growth and preparation method for microbial fertilizer | |
| CN104099282B (en) | Environment-friendly stalk degradation agents | |
| CN110330977A (en) | A kind of preparation method and soil conditioner of soil conditioner | |
| CN105315076A (en) | Anti-continuous-cropping compound microbial fertilizer dedicated to rhizoma dioscoreae | |
| CN107285925A (en) | A kind of method of utilization microorganism formulation stalk stack retting returning to the field |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220908 Address after: Room 607, North Building, No. 29, Meiling Road, Laoshan District, Qingdao City, Shandong Province, 266000 Patentee after: Deep Blue Grapefruit Fertilizer Industry (Qingdao) Co.,Ltd. Address before: North of Gongren Road, Jiangshan Industrial Park, Laixi City, Qingdao City, Shandong Province 266603 Patentee before: QINGDAO SHENLAN FERTILIZER INDUSTRY CO.,LTD. |